EP0351761A2 - Injecteur d'échantillons - Google Patents

Injecteur d'échantillons Download PDF

Info

Publication number
EP0351761A2
EP0351761A2 EP89113095A EP89113095A EP0351761A2 EP 0351761 A2 EP0351761 A2 EP 0351761A2 EP 89113095 A EP89113095 A EP 89113095A EP 89113095 A EP89113095 A EP 89113095A EP 0351761 A2 EP0351761 A2 EP 0351761A2
Authority
EP
European Patent Office
Prior art keywords
piston
injector
sample
plunger
nozzle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP89113095A
Other languages
German (de)
English (en)
Other versions
EP0351761B1 (fr
EP0351761A3 (fr
Inventor
Perry M. Ryno
Leigh J. Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems Inc
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Publication of EP0351761A2 publication Critical patent/EP0351761A2/fr
Publication of EP0351761A3 publication Critical patent/EP0351761A3/fr
Application granted granted Critical
Publication of EP0351761B1 publication Critical patent/EP0351761B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/022—Capillary pipettes, i.e. having very small bore
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

Definitions

  • This invention relates, in general, to liquid sample delivery and liquid chromatography and, in particular, to an apparatus for introducing liquid samples into an atomic absorption spectrometer.
  • Pipetting devices are shown in a number of U.S. Patents, for example, to Morrill, No. 3,200,650 and to Gerrarde, No. 3,040,494 and the textbook of Paul L. Kirk “Quantitative Ultra Micro Analysis", John Wiley and Sons, New York, 1950, pages 22-26, 73/864.02, teaches pipetting to determine sample volume.
  • the patents also show a source of gas to dispense the liquid sample.
  • Plunger type dispensing devices in connection with pipettes, are shown, for example, in the U.S. Patents to Avakian, No. 3,732,734, to Norton, 3,754,687, and to Parsons, 4,257,267.
  • the Avakian patent also shows a removable tip used with a pipette and the Parson patent shows a removable tip, which is called a pipette, but is not used to determine the volume of the liquid sample.
  • This invention comprises a disposable sample tip which is used to provide a liquid sample of a predetermined volume and a plunger type injector which is used to introduce the sample into a spectrometer with a precise amount of air pressure.
  • the sample tip comprises an outer tube for mounting the sample tip on the injector and an inner tube (pipette) of a desired bore and length.
  • the injector is provided with an anti-suction means so that the sample liquid will not be withdrawn from the inner tube by the injector in the event the inner tube is filled with the liquid sample before the injector is made ready to introduce the sample into the spectrometer.
  • FIG. 1 shows the sample tip enlarged relative to the injector 12 but figure 2 shows the relative sizes of the two devices and also shows the sample tip 10 mounted on the injector 12.
  • the sample tip 10 comprises an outer hollow tube 14 and a shorter inner capillary tube (pipette) 16 spaced from the inner wall 20 of the outer tube 14. Although shown as one molded piece, effectively one end of the inner tube 16 extends beyond the outer tube 14 to form an insertion tip 22 and a step 24 and the other end of the inner tube 16 terminates substantially midway of the outer tube 14. The length of the inner tube 16 and the size of its internal bore establishes the volume of the sample to be analyzed.
  • pipette inner capillary tube
  • Figure 3 illustrates the step of filling the inner tube 16 with liquid by inserting the sample tip 10 into a body of liquid 26 and figure 4 shows the step of introducing the sample into the spectrometer by inserting sample tip 10 into a graphite tube 30 of a spectrometer (not shown) with the step 24 establishing the distance between the end of the sample tip 10 and the graphite tube inner surface 32.
  • the injector 12 comprises a long tubular body 34 (handle) and a relatively thin walled hollow nozzle 36 which has a tubular portion 40 of the same outer diameter as the tubular body 34, a first taper 42 and, a second tubular portion 44 of a lesser diameter than tubular portion 40 which terminates in a slightly tapered narrow hollow point 46.
  • the hollow point 46 holds the sample tip 10 as shown in figures 5 and 6 and the tubular portion 40 forms a piston chamber 48.
  • the nozzle 36 is fitted over the end of the tubular body 34 and held thereon in any suitable manner as by internal and external complimentary threads 50.
  • the tubular body 34 has a central bore 52 in which a plunger 54 is slidably fit and which extends beyond the ends of the tubular body 34.
  • the lower end of the plunger 54 has a reduced portion 56 which is fitted with a retainer 60 which has a collar 62, a radially extended disc 64 and a downwardly extending short connecting rod 66.
  • the retainer 60 is held on the plunger 54 by a lock pin 70 and lock washer 72 holds a hollow, relatively thin walled, downwardly opening piston 74 on the connecting rod 66. Both the piston 74 and the retainer 60 are enclosed in the piston chamber 48 where movement of the piston 74 downwardly will expell a measured amount of air under pressure from the nozzle point 46 and dispense the sample from the sample tip 10.
  • a counterbore 76 in the lower end of the tubular body 34 encloses a helical piston spring 78 which biases the piston 74 toward a downward or uncocked position.
  • the upper end of the counterbore 76 is open to atmosphere by radial holes 80 (Figs 5 & 6).
  • the spring 78 and the piston 74 are held in an upper, or cocked, position by a latching mechanism 82. (See the enlarged view of the latch mechanism 82 in figures 9 and 10).
  • This latching mechanism 82 comprises a latch button 84 with an extension arm 86 of a lesser diameter and containing an opening 90 which allows the plunger 54 to pass therethrough.
  • the extension arm 86 is disposed in sliding relationship within a sleeve 92 and a blind radial counterbore 94 in the tubular body 34. In one position, the cocked position, an edge 96 on the extension arm 86 near the opening 90 engages a shoulder 100 on the plunger 54 formed by a reduced radius 102 in the area of the latch mechanism 82 and, in another position, the uncocked position, allows the plunger 54 to move freely in response to the piston spring 78.
  • the extension arm 86 is spring biased toward the cocked position by a helical spring 104.
  • a handle 106 attached to the upper end of the plunger 54, is provided for the purpose of pulling the plunger upwardly and is operable manually.
  • the latching mechanism 82 will automatically latch the piston 54 in the cocked position when the handle 106 is pulled upwardly a sufficient distance.
  • the plunger 54 and piston 74 are released by pressing the latch button 84 radially inwardly against the force of the spring 104 which will free the shoulder 100 of the edge 96 and allow the helical spring 78 to move the piston 74 downwardly to create a sufficient amount of air under pressure to be expelled from the nozzle 40 and to expel the sample from the sample tip 10 as shown schematically in figure 4.
  • the handle 106 extends into a blind bore 110 in the top of the tubular body 34 in sliding relationship and the bottom 112 of the handle 106 engages the bottom 114 of the blind bore 110 to limit the stroke of the piston 74.
  • the recommended procedure is to cock the injector 12 either before and after the sample tip 10 is mounted on the injector 12 but before the sample tip 10 is inserted into the liquid to be sampled.
  • the injector 12 is provided with an anti-suction means 120 which is a function of the retainer 60 and the piston 74 and which will now be described in detail. This is considered an important feature of this invention and figures 7 and 6 show its operation.
  • the top wall 122 of the piston 74 contains holes 124 (2 shown) of a relatively small size. These small holes 124 form passage means for air on one side of the piston 74 to flow to the other side of the piston 74 and the disc 64 forms a closure means for opening and closing these holes 124 during movement of the piston 74.
  • the words “upper” or “lower”, “down” or “up”, or “left” or “right” were used to facilitate the description of the invention with reference to the drawings.
  • the words “bore” and “counterbore” are used, they were not meant to imply that these were necessarily formed by a machining process since the injector (and many of its parts) and the sample tip may be formed of a suitable commercially available plastic by a molding process.
  • the liquid of the sample to be analyzed may be any liquid, but in this case, lubricating oil is the liquid for which this invention is particularly directed so that wear of movable metal parts, such as aircraft engine parts, may be determined by atomic absorption spectrometry.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP89113095A 1988-07-18 1989-07-17 Injecteur d'échantillons Expired - Lifetime EP0351761B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22071888A 1988-07-18 1988-07-18
US220718 1988-07-18

Publications (3)

Publication Number Publication Date
EP0351761A2 true EP0351761A2 (fr) 1990-01-24
EP0351761A3 EP0351761A3 (fr) 1991-02-13
EP0351761B1 EP0351761B1 (fr) 1993-09-08

Family

ID=22824661

Family Applications (1)

Application Number Title Priority Date Filing Date
EP89113095A Expired - Lifetime EP0351761B1 (fr) 1988-07-18 1989-07-17 Injecteur d'échantillons

Country Status (5)

Country Link
EP (1) EP0351761B1 (fr)
JP (1) JP2960438B2 (fr)
KR (1) KR0140888B1 (fr)
CA (1) CA1336970C (fr)
DE (1) DE68908979T2 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0514678A1 (fr) * 1991-04-30 1992-11-25 Shimadzu Corporation Procédé pour le positionnement d'essai et dispositif pour la spectrophotométrie d'absorption atomique de flamme
US5311444A (en) * 1991-06-30 1994-05-10 Shimadzu Corporation Absorbance analyzer and data processing method for chromatograph
EP0636874A1 (fr) * 1993-07-28 1995-02-01 Bio Merieux Procédé de microdosage d'un liquide pour l'obtention des dilutions nanovolumétriques
CN113671108A (zh) * 2021-08-03 2021-11-19 苏州鼎实医疗科技有限公司 一种气压平衡结构及具有其的定量采样加注器

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002272462A (ja) * 2001-03-19 2002-09-24 Shimadzu Corp 核酸試料の精製カラム及び精製方法
JP4224488B2 (ja) * 2005-11-14 2009-02-12 株式会社アイディエス 検体分取分注用ノズル装置
WO2020110173A1 (fr) * 2018-11-26 2020-06-04 ムロオカ産業株式会社 Distributeur

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3334788A (en) * 1963-08-13 1967-08-08 Clark H Hamilton Syringe for chromatographic analyses
FR2447216A1 (fr) * 1979-01-23 1980-08-22 Suovaniemi Finnpipette Pipette a doses multiples
EP0082263A1 (fr) * 1981-12-10 1983-06-29 The Perkin-Elmer Corporation Introduction d'échantillon pour analyse par spectrométrie ou chromatographie liquide
EP0232517A2 (fr) * 1985-12-12 1987-08-19 Glasgerätebau Hirschmann Pipette

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3334788A (en) * 1963-08-13 1967-08-08 Clark H Hamilton Syringe for chromatographic analyses
FR2447216A1 (fr) * 1979-01-23 1980-08-22 Suovaniemi Finnpipette Pipette a doses multiples
EP0082263A1 (fr) * 1981-12-10 1983-06-29 The Perkin-Elmer Corporation Introduction d'échantillon pour analyse par spectrométrie ou chromatographie liquide
EP0232517A2 (fr) * 1985-12-12 1987-08-19 Glasgerätebau Hirschmann Pipette

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0514678A1 (fr) * 1991-04-30 1992-11-25 Shimadzu Corporation Procédé pour le positionnement d'essai et dispositif pour la spectrophotométrie d'absorption atomique de flamme
US5479253A (en) * 1991-04-30 1995-12-26 Shimadzu Corporation Sample transferring method and apparatus for flame type atomic absorption spectrophotometry
US5311444A (en) * 1991-06-30 1994-05-10 Shimadzu Corporation Absorbance analyzer and data processing method for chromatograph
EP0636874A1 (fr) * 1993-07-28 1995-02-01 Bio Merieux Procédé de microdosage d'un liquide pour l'obtention des dilutions nanovolumétriques
FR2708341A1 (fr) * 1993-07-28 1995-02-03 Bio Merieux Procédé de dosage, notamment micro-dosage d'un liquide; application à l'obtention de dilutions contrôlées, notamment des dilutions nanovolumétriques.
US5594183A (en) * 1993-07-28 1997-01-14 Bio Merieux Process for metering, in particular microvolumes of a liquid; application to obtaining controlled dilutions, especially nanomolar dilutions
CN113671108A (zh) * 2021-08-03 2021-11-19 苏州鼎实医疗科技有限公司 一种气压平衡结构及具有其的定量采样加注器
CN113671108B (zh) * 2021-08-03 2023-11-28 苏州鼎实医疗科技有限公司 一种气压平衡结构及具有其的定量采样加注器

Also Published As

Publication number Publication date
EP0351761B1 (fr) 1993-09-08
CA1336970C (fr) 1995-09-12
EP0351761A3 (fr) 1991-02-13
DE68908979T2 (de) 1994-01-05
JPH02257033A (ja) 1990-10-17
KR0140888B1 (ko) 1998-06-01
KR900002069A (ko) 1990-02-28
DE68908979D1 (de) 1993-10-14
JP2960438B2 (ja) 1999-10-06

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