Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
  • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
  • A01H4/001—Culture apparatus for tissue culture

Definitions

• the invention relates to an arrangement for micropropagation of plants including an aseptical space and sterilized cultivating vessels. It is known that the methods commonly defjned as belonging to the biotechnology stand in the forefront of the appl ied biological research works and of the related scientific investigations. The biotechnological methods are especially important in propagation of tissues and cellular elements of the plants, wherein the propagation is applied also for investigating the plant systems.
• the common feature of the micropropagation methods for propagating tissues and cel lular elements is that they are carried out in sterile conditions, in steri lized cultivating vessels, in vitro, in order to clarify the biological aspects.
• the micropropagation of plants includes the foIlowing steps:
• the steps mentioned above require generally the same means and pieces of equipment as in the microbiology, i.e. glass vessels for receiving a culture-medium including a cultivating component and a solidifying component, said culture-medium being warmed up.
• the glass vessels are closed by a cotton-wool cork, whereby the respiration of the cultures is ensured but the sporules can not enter the vessels.
• the cotton-wool cork is usually closed by an aluminium foil.
• the transplantation is carried out in inoculation boxes wherefrom the products of the operation carried out in sterile conditions are transported to the place of cultivation and incubation.
• the glass vessels have been replaced in some institute by vessels made of light transparent plastics and the cotton-wooI applied for closing can be substituted by corks made of foamed plastics, covering elements made of plastics, parafiIm or by PVC foil.
• the object of the invention is to provide a method whereby the efficiency of the micropropagation can be improved with fulfilling the streng biological purity requirements similarly to the known solutions.
• the invention is based on the recognition that it is possible to realise an equipment of high efficiency for micropropagation of plants when the sequence of the traditional operations of micropropagation is changed in the way that after preparing the culture-medium the following operations are carried out in sterile conditions: sterilizing the medium, feeding the necessary components into the vessel, transplanting the plants and closing the vessel. The incubation does not require sterile conditions.
• the object of the invention is therefore an arrangement for micropropagation of plants, including an aseptical space, sterilized cultivating vessels, a storing unit for receiving a feeding apparatus for forwarding the cultivating vessels, means e.g.
• an ultraviolet lamp for ensuring sterility of the cultivating vessels in the storing unit
• conveyor means arranged under the feeding apparatus or in the environment thereof, storage means for receiving sterilized breeding components, storing means for receiving sterilized solidifying components, at least one feeding head connected to the storage means and the storing means, a mixing head connected to the at least one feeding head including an outlet opening, thermostating means for ensuring required temperature of the storage means, and the storing means preferably of the at least one feeding head and the mixing head means for sterilized closing the cultivating vessels comprising a first group of a supporting element for receiving a closing foil arranged over the conveyor means, cylindr ⁇ c forwarding means for forwarding the closing foil, a heating filament lying near to the cylindric forwarding means and a heat treatment unit and or a second group of a storage unit for receiving closing covers, feeding unit for forwarding the closing covers, means for ensuring sterility of the closing covers and if desired closing means for fixing the closing covers on the cultivating vesseIs.
• the arrangement proposed by the invention preferably applies conveyor means with clawed surface, a flexible tube element for connecting the mixing head to the at least one feeding head and a slide arranged under the feeding apparatus.
• multidirectional guiding means arranged at the output of the conveyor means and further conveyor means coupled to the guiding means having preferably c I awe d s u r f ace .
• Figure 1 shows the arrangement of the invention in side view
• Figure 2 il lustrates the arrangement of the Figure 1 in top view.
• the arrangement as proposed by the invention is a laboratory device realised with closed outer surface for ensuring an aseptical space. This outer surface can be created e.g. by a laminar box, a laminary tunnel or by a laminary chamber.
• the arrangement as proposed makes use of steri lized cultivating vessels made of light transparent or not transpare plastics as poliethylen, poI ipropyIen, poI icarbonate etc. They are preferably rectangular boxes or vessels of other shapes which can be placed in one another.
• the cultivating vessels are arranged in a storing unit 1 lyint in one another or in a reel.
• a feeding apparatus 2 is connected to the output of the storing unit 1 ( Figure 1) wherein means 3 ensure sterility of the cultivating vessels.
• the means 3 can be formed e.g. by an ultraviolet lamp.
• a slide 4 connects the output of the feeding apparatus 2 with conveyor means 5 for transporting the cultivating vessels to further operation stages.
• multidirectional guiding means 10 can be arranged at the end of the conveyor means 5 for selecting the cultivating vessels according to different directions of forwarding. In this case further conveyor means 11 are foreseen.
• the cultivating are transported before the output of a mixing head 8 receiving steri lized breeding components and steri l ized sol idifying components from storage means 61 and storing means 62 respectively, over at least one feeding head 71, 72 ( Figure 2).
• the storage means 61 and the storing means 62 are arranged in respective thermostat ing means 91, 92 ensuring their required temperature.
• the cultivating vessels filled with the components of the culture-medium are forwarded to means for sterilized closing them. This is realised in an appropriate way, e.g.
• closing covers arranged in a storage unit 15 equipped with means 17 for ensuring sterility of the closing covers and with feeding unit for forwarding the closing covers to the vessels to be closed.
• the closing covers are pressed onto the cultivating vessels by closing means 18, if this is required.
• the sterile connection between the cultivating vessel and the PVC foil is ensured by a heating unit 19 for blowing heat air.
• the closing PVC foil is arranged on a supporting element 12.
• the foil can be made of other appropriate materials, if required.
• the next step is to place the cultivating vessels in the storing unit 1 and to connect the feeding head 71 with the storage means 61 comprising the sterilized breeding components.
• the sterilized solidifying components comprised in the storing means 62 is be warmed up to the liquid state and then in the aseptical space the feeding head 72 can be connected to the storing means 62.
• the feeding heads 71, 72 should be coupled with the mixing head 8, e.g. by a flexible tube element.
• the storage units prepared in this way can be placed in the thermostating means 91, 92 ensuring their required temperature.
• the supporting means 12 are, if necessary, equipped with rol led PVC foil, the free end of the foil has to be fixed on the surface of the cyl indric forwarding means 13, in the near environment of the heating filament 14. If applied, the storage unit 15 should be fil led up with closing covers made of appropriate plastics, lying over one another. The arrangement is connected thereafter to a control unit for regulating the operation conditions.
• the operations are the fol lowing:
• the feeding apparatus 2 lets a cultivating vessel move to the output opening of the mixing head 8. In this operation the si ide 4 can be helpful.
• the mixing head 8 receives the necessary components in required amount from the storage unit 61 and storage means 62 over the feeding heads 71, 72.
• the solidifying component makes the culture-medium solidify.
• the conveyor means 5 transport the cultivating vessels to the multidirectional guiding means 10, where the transplantation can be carried out.
• the further conveyor means 11 transport the cultivating vessel to the fol lowing operations.
• transplantation can be carried out in the cultivating vessel during transporting it on the surface of the conveyor means 5, making use of a short stop thereof.
• the multidirectional guiding means 10 are not necessary.
• the conveyor means 5 or 11 forward the cultivating vessels to the means for steril ized closing them.
• the cultivating vessel stops underneath the cyl indric forwarding means 13 wherefrom the PVC foil is pressed to the vessel.
• the heating filament 14 cuts the foi l and in a heat treatment unit 19 under the influence of relatively high temperature the foi I is tensioned on the upper part of the cultivating vessel. In this way the sterile conditions are fully realised.
• Another preferred solution is to apply closing covers made of plastics.
• the feeding unit 16 forwards the covers to the upper surface of the vessel where they are pressed to the vessel by the closing means 18.
• the cultivating vessels closed in the aseptic space are taken away from the inner parts of the arrangement, wherein the closing means applying plastic covers and foil can be applied also simultaneously.
• the closing covers are made in this case with perforation or other lacks of material as requied in the following operational steps.

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• Life Sciences & Earth Sciences (AREA)
• Engineering & Computer Science (AREA)
• Biotechnology (AREA)
• Developmental Biology & Embryology (AREA)
• Cell Biology (AREA)
• Botany (AREA)
• Environmental Sciences (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Applications Claiming Priority (1)

Publications (1)

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Family Applications (1)

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• 1987
  • 1987-03-06 DE DE873790915T patent/DE3790915T1/de active Pending
  • 1987-03-06 JP JP62501744A patent/JPH01503035A/ja active Pending
  • 1987-03-06 DE DE3790915A patent/DE3790915C2/de not_active Expired - Fee Related
  • 1987-03-06 WO PCT/HU1987/000010 patent/WO1988006618A1/en not_active Application Discontinuation
  • 1987-03-06 EP EP87901607A patent/EP0304417A1/de not_active Withdrawn
  • 1987-03-06 NL NL8720126A patent/NL8720126A/nl not_active Application Discontinuation
• 1988
  • 1988-11-07 GB GB8826044A patent/GB2209535A/en not_active Withdrawn

Non-Patent Citations (1)

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