EP0271153B1 - Composition détergente à base d'enzymes - Google Patents

Composition détergente à base d'enzymes Download PDF

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Publication number
EP0271153B1
EP0271153B1 EP87202385A EP87202385A EP0271153B1 EP 0271153 B1 EP0271153 B1 EP 0271153B1 EP 87202385 A EP87202385 A EP 87202385A EP 87202385 A EP87202385 A EP 87202385A EP 0271153 B1 EP0271153 B1 EP 0271153B1
Authority
EP
European Patent Office
Prior art keywords
lipase
lipases
pseudomonas
detergent
detergent composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP87202385A
Other languages
German (de)
English (en)
Other versions
EP0271153A2 (fr
EP0271153A3 (en
Inventor
Hendrik Egbert De Jong
Ton Swarthoff
David Thom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever PLC
Unilever NV
Original Assignee
Unilever PLC
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever PLC, Unilever NV filed Critical Unilever PLC
Publication of EP0271153A2 publication Critical patent/EP0271153A2/fr
Publication of EP0271153A3 publication Critical patent/EP0271153A3/en
Application granted granted Critical
Publication of EP0271153B1 publication Critical patent/EP0271153B1/fr
Anticipated expiration legal-status Critical
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase

Definitions

  • the present invention relates to an enzymatic detergent composition
  • an enzymatic detergent composition comprising as detergent active material solely a nonionic synthetic detergent, and a mixture of lipolytic and proteolytic enzymes.
  • Lipolytic enzymes are well known in detergent compositions. Lipolytic enzymes have been mentioned in the prior art as possible enzymes for detergent compositions, but there is relatively little prior art directly concerned with-lipases for inclusion in detergent compositions.
  • nonionic synthetic detergents in general do not negatively influence the activity of proteolytic and lipolytic enzymes, in contrast with e.g. anionic synthetic detergents, which often negatively influence the proteases and lipases.
  • lipases are basically proteins, they would be susceptible to proteolytic action by the proteases if lipases were used in conjunction with proteases. It would therefore be expected that the inclusion of proteases in a detergent composition comprising lipases and as detergent-active material solely a nonionic detergent surfactant (which does not affect either the lipase or the protease), would cause the lipase activity to be destroyed by the proteolytic action of the proteases.
  • the class of lipases to be used according to the present invention embraces those lipases which show a positive immunological cross-reaction with the antibody of the lipase produced by the microorganism Chromobacter viscosum var. lioolvticum NRRL B 3673.
  • This lipase has been described in Dutch Patent Specification 154,269 of Toyo Jozo KK, and the microorganism is available to the public from the collection of the United States Department of Agriculture, Agricultural Research Service, Northern Utilization and Development Division, Peoria, Illinois, under N ° NRRL B 3673.
  • This lipase will be referred to hereinafter as "Toyo Jozo" lipase.
  • the lipases of the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodif- fusion procedure according to Ouchterlony (Acta. Med. Scan., 133, pages 76-79 (1950)).
  • the preparation of the antiserum is carried out as follows:
  • the serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
  • the titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 25 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
  • lipases showing a positive immunological cross-reaction with the Toyo Jozo lipase antibody as hereabove described are lipases according to the present invention. Typical examples thereof are the lipase ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas- nitroreducens var. lioolvticum FERM P 1338, the lipase ex Pseudomonas sp.
  • the lipase ex Pseudomonas fluorescens IAM 1057 available under the trade name Amano-P
  • the lipase ex Pseudomonas fragi FERM P 1339 available under the trade name Amano-B
  • Chromobacter viscosum e.g. Chromobacter viscosum var. lioolvticum NRRL B 3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further C. viscosum lipases from US Biochemical Corp., U.S.A., and Diosynth Co., The Netherlands, and lipases ex Pseudomonasaladioli.
  • the lipases of the present invention are included in the detergent composition in such an amount that the final detergent composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg, preferably 25 to 0.05 LU/mg of the composition.
  • lipases can be used in their non-purified form or in a purified form, e.g. purified with the aid of well-known adsorption methods, such as phenyl sepharose adsorption techniques.
  • proteases used according to the present invention are the well-known detergent proteases and mixtures thereof.
  • the proteases are used in such an amount that the final detergent composition has a proteolytic activity of 0.1 to 50 GU/mg, preferably 0.5 to 30 GU/mg.
  • GU Glycine Unit; one GU is the amount of enzyme which under standard incubation conditions produces an amount of terminal NH 2 -groups equivalent to 1 microgr./ml glycine.
  • the detergent composition further comprises as detergent-active material solely a nonionic synthetic detergent.
  • This type of detergent is well known in the art.
  • Nonionics usually consist of a hydrophobic moiety derived from fatty alcohols, fatty acid amides and alkylphenols, and a hydrophilic moiety consisting of alkylene oxide units. Typical examples are the condensation products of alkylene oxides such as ethylene oxide, propylene oxide and butylene oxide and mixtures thereof with C ⁇ -Cz primary or secondary, branched or straight chain alcohols, C 8 -C 18 fatty acid amides, C 9 -C 18 alkylphenols, etc. Further suitable examples are given inM. Schick "Nonionic surfactants" (1967).
  • the composition contains from 1-50%, usually from 2-45%, and commonly from 5-30% by weight of the nonionic detergent. Mixtures of various nonionic detergents may also be used.
  • the detergent composition may furthermore include the usual detergent ingredients in the usual amounts.
  • the composition may contain from 1-60%, preferably from 5-30% by weight of one or more organic and/or inorganic builders. Furthermore, it may contain from 1-35% of a bleaching agent or a bleaching system comprising a bleaching agent and an activator therefor, e.g. sodium perborate with tetraacetyl ethylene diamine.
  • compositions may furthermore comprise lather boosters, foam depressors, anti-corrosion agents, soil-suspending agents, sequestering agents, anti-soil redeposition agents, perfumes, dyes, solvents, stabilising agents for the enzymes and bleaching agents and so on. They may also comprise from 0.01-10% by weight of enzymes other than lipases and proteases, such as amylases, oxidases and cellulases.
  • compositions of the present invention can be formulated in any desired form, such as powders, bars, pastes, liquids, etc.
  • the detergent composition used had the following formulation:
  • the reflectance of the test cloths and the residual weight percentage of fatty material on the test cloths were determined after the fourth wash cycle.
  • the reflectance was measured in a Reflectometer at 460 nm with a UV filter in the light pathway, and the fatty matter by extracting the dried test cloths with petroleum ether, and determining the amount of fatty matter from the weight loss of the test cloth.
  • the stability of lipases according to the present invention in the presence of proteases and varying amounts of a nonionic detergent was tested by determining the residual lipase activity with the pH-stat. method.
  • a composition comprising 0.7 g pentasodium triphosphate, 0.7 g sodium sulphate and 0.2 sodium silicate and varying amounts of C 13 -C 15 alcohol, condensed with 7 and 3 moles of ethylene oxide, respectively (product A and product B) was used.
  • the temperature was 30 ° C, the water hardness 14 ° FH and the pH 9.5.
  • the lipase was either lipase ex Pseudomonas gladioli or Amano-P, at 15 LU/ml, and the protease was Savinase, at 20 GU/ml.
  • Example 1 was repeated, using various lipases. The following results were obtained:

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Detergent Compositions (AREA)

Claims (2)

1. Composition détergente comprenant de 1 à 50% en poids d'un ou plusieurs surfactifs détergents non ioniques à titre du seul surfactif détergent, des protéases en une quantité telle que la composition détergente possède une activité protéolytique de 0,1 à 50 Unités de Glycine par mg, et des lipases en une quantité telle que la composition détergente possède une activité lipolytique de 0,005 à 100 Unités de Lipase par mg, les lipases étant celles qui font preuve d'une réaction croisée immunologique positive avec l'anti-corps de la lipase produit par Chromobacter viscosum var. lipolyticum NRRL-B 3673.
2. Composition selon la revendication 1, dans laquelle la lipase est obtenue à partir de Pseudomonas fluorescens, Pseudomonas fraai, Pseudomonas nitroreducens var. lipolyticum Pseudomonas ceoacia, Pseudomonas aladioli et Pseudomonas viscosum.
EP87202385A 1986-12-10 1987-12-02 Composition détergente à base d'enzymes Expired - Lifetime EP0271153B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8629535 1986-12-10
GB868629535A GB8629535D0 (en) 1986-12-10 1986-12-10 Enzymatic detergent composition

Publications (3)

Publication Number Publication Date
EP0271153A2 EP0271153A2 (fr) 1988-06-15
EP0271153A3 EP0271153A3 (en) 1988-08-10
EP0271153B1 true EP0271153B1 (fr) 1990-03-14

Family

ID=10608785

Family Applications (1)

Application Number Title Priority Date Filing Date
EP87202385A Expired - Lifetime EP0271153B1 (fr) 1986-12-10 1987-12-02 Composition détergente à base d'enzymes

Country Status (9)

Country Link
EP (1) EP0271153B1 (fr)
JP (1) JPH0696717B2 (fr)
AU (1) AU605806B2 (fr)
BR (1) BR8706682A (fr)
CA (1) CA1288368C (fr)
DE (1) DE3761910D1 (fr)
ES (1) ES2014465B3 (fr)
GB (1) GB8629535D0 (fr)
ZA (1) ZA879296B (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0218272B1 (fr) * 1985-08-09 1992-03-18 Gist-Brocades N.V. Enzymes lipolytiques et leur usage dans des compositions détergentes
GB8629536D0 (en) * 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition
DK571587D0 (da) * 1987-11-02 1987-11-02 Novo Industri As Enzymatisk detergentsammensaetning
BE1001436A3 (fr) * 1988-02-22 1989-10-31 Synfina Sa Nouvelle lipase et compositions detergentes en contenant.
US5733763A (en) * 1988-08-19 1998-03-31 Novo Nordisk A/S Enzyme granulate formed of an enzyme-containing core and an enzyme-containing shell
GB8826110D0 (en) * 1988-11-08 1988-12-14 Unilever Plc Enzyme-containing detergent compositions
DK78189D0 (da) * 1989-02-20 1989-02-20 Novo Industri As Enzymholdigt granulat og fremgangsmaade til fremstilling deraf
US4950417A (en) * 1989-05-01 1990-08-21 Miles Inc. Detergent formulations containing alkaline lipase derived from Pseudomonas plantarii
GB8915658D0 (en) * 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
JPH0489897A (ja) * 1990-07-30 1992-03-24 Solvay Enzyme Prod Inc アルカリ性リパーゼを含む洗剤配合物
WO1994007984A1 (fr) * 1992-09-25 1994-04-14 The Procter & Gamble Company Composition de detergent contenant un dispersant de savon de chaux et des lipases
ATE211163T1 (de) * 1992-09-25 2002-01-15 Procter & Gamble Verwendung eines kalksteindispergiermittels in einer waschmittelzusammensetzung enthaltend lipase enzyme
US5772786A (en) * 1993-08-13 1998-06-30 The Procter & Gamble Company Detergent composition comprising lime soap dispersant and lipase enzymes
DE19954181A1 (de) * 1999-11-10 2001-05-31 Eppendorf Geraetebau Netheler Verwendung nichtionogener Tenside zur Durchführung enzymatischer Reaktionen
EP2529998B1 (fr) 2009-12-28 2016-03-30 Toyota Jidosha Kabushiki Kaisha Structure de capot de véhicule
CN110804506B (zh) * 2019-11-13 2021-06-01 领先生物农业股份有限公司 一种微生物毛洗涤剂及其使用方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1619087A1 (de) * 1967-08-14 1969-10-02 Henkel & Cie Gmbh Als Textilwaschmittel brauchbare Tensidkombinationen sowie diese enthaltende Wasch- oder Waschhilfsmittel
GB1273545A (en) * 1968-06-24 1972-05-10 Albright & Wilson Multi-enzyme cleaning compositions
DK289083A (da) * 1983-06-23 1984-12-24 Novo Industri As Lipase, fremgangsmaade til fremstilling deraf og anvendelse deraf
GB8514708D0 (en) * 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
GB8514707D0 (en) * 1985-06-11 1985-07-10 Unilever Plc Enzymatic detergent composition
DK154572C (da) * 1985-08-07 1989-04-24 Novo Industri As Enzymatisk detergentadditiv, detergent og fremgangsmaade til vask af tekstiler
GB8629536D0 (en) * 1986-12-10 1987-01-21 Unilever Plc Enzymatic detergent composition

Also Published As

Publication number Publication date
GB8629535D0 (en) 1987-01-21
JPH0696717B2 (ja) 1994-11-30
AU8222687A (en) 1988-06-16
CA1288368C (fr) 1991-09-03
DE3761910D1 (de) 1990-04-19
AU605806B2 (en) 1991-01-24
JPS63161084A (ja) 1988-07-04
BR8706682A (pt) 1988-07-19
ZA879296B (en) 1989-08-30
EP0271153A2 (fr) 1988-06-15
EP0271153A3 (en) 1988-08-10
ES2014465B3 (es) 1990-07-16

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