EP0214043A1 - Instrument and process for artificial insemination, in utero embryo transplants or taking follicular liquid by transperitoneal means in animals - Google Patents

Instrument and process for artificial insemination, in utero embryo transplants or taking follicular liquid by transperitoneal means in animals Download PDF

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Publication number
EP0214043A1
EP0214043A1 EP86401825A EP86401825A EP0214043A1 EP 0214043 A1 EP0214043 A1 EP 0214043A1 EP 86401825 A EP86401825 A EP 86401825A EP 86401825 A EP86401825 A EP 86401825A EP 0214043 A1 EP0214043 A1 EP 0214043A1
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Prior art keywords
instrument
needle
catheter
instrument according
embryos
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EP86401825A
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German (de)
French (fr)
Inventor
Yves Cognie
Jean Charles Edmond Vallet
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Institut National de la Recherche Agronomique INRA
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Institut National de la Recherche Agronomique INRA
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/027—Devices for injecting semen into animals, e.g. syringes, guns, probes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation

Definitions

  • the invention relates to a method and an instrument for artificial insemination, transplantation of intrauterine embryos or the puncture of follicular fluids, transperitoneally, in animals, in particular sheep, goats, cattle, horses or others.
  • sheep and goats we know the difficulties encountered during artificial insemination or the transfer of embryos by natural means.
  • the body of the uterus being difficult to cross in goats and, impassable in sheep, we were therefore forced, until then, to carry out a surgical intervention, under general anesthesia, with the drawbacks that this generates from the medical point of view and economical.
  • Such a surgical operation supposes, in fact, the existence of sophisticated equipment which is obviously not suited to the flexibility required for field techniques. To this investment is added anesthesia costs as well as post-operative care, which significantly increases the cost of the intervention.
  • the method according to the invention is characterized in that one makes in the abdominal wall of the animal, fine close incisions, one being intended to receive an endoscope making it possible to view the operating field, the other being intended the passage of a trocar provided with a guide sleeve, the withdrawal of this trocar, after perforation, allowing the passage, in the guide sleeve remaining in place, of an insemination, transplantation or follicular fluid removal instrument , presenting the originality of being connected to a suction means and also comprising, a injection syringe to allow on the one hand, the fixing, by suction effect, of the wall of one of the two uterine horns and, on the other hand, to cross the endometrium to bring the needle into the lumen of the horn to be treated, in order to deposit the semen or the embryos therein or to practice the desired samples, after which the second horn is treated, still under endoscopy, after withdrawal of the needle and new orientation of the instrument.
  • the instrument for implementing this process consists of a rigid probe formed by two separate coaxial conduits, one connected to a suction pump, the other serving as a guide for a punctate catheter whose base is radially slotted so that the operator can view the movement of the needle and in particular control its introduction into the lumen of the horn.
  • the catheter consists of a syringe comprising on the one hand a sheath, closed at one of its ends by an end piece extended by a needle, said end piece comprising a frustoconical internal cavity, on the other hand a sliding piston with seal in this sheath.
  • the catheter contains a fine straw for conditioning the semen or embryos, made integral with the tip by force fitting into its frustoconical cavity, the injection then being carried out by the sliding piston placed in support , by its front end, on the plug usually closing this type of glitter.
  • the essential object of the invention is to provide practitioners with flexible technology and instrumentation, of simple design, which are nevertheless of high reliability and at the lowest possible cost.
  • two incisions 1 and 2 are made on the abdomen of the animal (approximately in the vicinity of what is called the "white line” for sheep and goats and on the flank for cattle and horses). 2, fine and close together, and an endoscope 3 is introduced into one of them, making it possible to visualize the operating field.
  • the abdominal wall and the peritoneum are then punctured using a trocar, conventionally constituted by a mandrel (not shown) and its guide sleeve 4 which, after removal of the mandrel, will serve as a sheath for the introduction and guiding the specific instrument 5 of the invention.
  • this instrument is similar to a rigid probe comprising two tangential channels 51, 52 ( Figure 2), these being defined by two tubes 6 and 7, of different diameters, housed one in the other and joined at their two ends by brazing, the conduit 51 being separated from the conduit 52 and comprising a radial notch 53 for viewing the needle.
  • the external channel 52 is connected by connection 8 to a suction means, while the internal channel 51 serves as a guide for a punctiform catheter 9 formed essentially (FIG. 4) of a sheath 10, the base of which is closed by a nozzle. 11 extended by a needle 12, this sheath introduced into the channel 51 receiving a piston 13, calibrated using marks 13 de, the head of which slides with sealing in the inner duct of the sheath.
  • the catheter in the simplest version, in particular when injecting fresh seeds or immediately transplanting embryos, the catheter consists, as described above, of a simple needle sheath and a injection piston.
  • fine flakes 14 are used, which are fixed inside the catheter, and the closure plug 15 of which serves as a piston.
  • these fine flakes are used for the conservation, in cryogenic medium, of seeds or embryos and that they are formed of a capillary tube welded at one of its ends and closed at the other by a plug containing a powder. polymerizable on contact with the substance.
  • the anterior end must be cut off and forced into the conical cavity 16 of the catheter tip in order to immobilize it. It then suffices to use the piston 13, after having removed its removable head, so that the latter can slide in the fine straw and come into abutment on the plug 15 which then acts as the piston head.
  • the catheter contains two doses of fifteen million sperm each to treat the two horns 20.
  • a dose has been injected, it suffices to partially withdraw the catheter and in particular to needle 12, then tilt, under endoscopy, the instrument, to bring its front end facing the second horn, then repeat the operations.
  • the method and the instrument according to the invention have many advantages, because to the finesse of the surgery used and its safety, are added those linked to the flexibility and the economic nature of the intervention. Indeed, the simple design instrument makes it possible to treat animals directly in the field, since we no longer have recourse to the facilities specific to heavy surgery and general anesthesia. In addition, the incisions being fine, the animal is not very stressed and the post-operative care practically non-existent. Finally, and this is a particularly important advantage in the context of artificial insemination of sheep and goats, we can set up using this instrumentation, a low number of spermatozoa (thirty million for the two horns) where it was needed, with traditional techniques 400 million spermatozoa.
  • this instrument is particularly well suited to embryo transfer and the removal of follicular fluids from sheep, goats, cattle or horses, only the dimensions of the device changing according to the size of the body. 'animal.
  • this instrument is easy to handle because the operator can hold the endoscope and the probe with one hand, which includes a gripping ring 25 for this purpose.

Abstract

The instrument is used in particular on the ovine, caprine, bovine or equine species and is characterised in that it consists of a rigid probe (5) comprising two tangential channels (51, 52) defined by two conduits of different diameters, lodged one inside the other, one of the conduits (52) being connected to a suction pump, the other (51) serving as a guide for a punctiform catheter, the base of the latter being notched radially in order to permit visualisation, with endoscopy, of the displacement of the needle (12). <IMAGE>

Description

L'invention concerne un procédé et un instrument pour l'insémination artificielle, la transplantation d'embryons in-utéro ou la ponction de liquides folliculaires, par voie transpéritonéale, chez les animaux, notamment les ovins, caprins, bovins, équins ou autres.The invention relates to a method and an instrument for artificial insemination, transplantation of intrauterine embryos or the puncture of follicular fluids, transperitoneally, in animals, in particular sheep, goats, cattle, horses or others.

S'agissant des ovins et des caprins, on sait les dif­ficultés que l'on rencontre lors de l'insémination artificielle ou du transfert d'embryons par les voies naturelles. Le corps de l'utérus étant difficilement franchissable chez les caprins et, infranchissable chez les ovins, on était donc contraint, jus­qu'alors, de procéder à une intervention chirurgicale, sous anes­thésie générale, avec les inconvénients que cela engendre du point de vue médical et économique. Une telle opération chirurgicale suppose, en effet, l'existence d'un matériel sophistiqué qui n'est évidemment pas adpaté à la souplesse requise pour les techniques de terrain. A cet investissement vient s'ajouter les frais d'anes­thésie ainsi que les soins post-opératoires, ce qui grève sensi­blement le coût de l'intervention.With regard to sheep and goats, we know the difficulties encountered during artificial insemination or the transfer of embryos by natural means. The body of the uterus being difficult to cross in goats and, impassable in sheep, we were therefore forced, until then, to carry out a surgical intervention, under general anesthesia, with the drawbacks that this generates from the medical point of view and economical. Such a surgical operation supposes, in fact, the existence of sophisticated equipment which is obviously not suited to the flexibility required for field techniques. To this investment is added anesthesia costs as well as post-operative care, which significantly increases the cost of the intervention.

Pour remédier à ces inconvénients, on fait appel, selon l'invention, à un procédé et un instrument de conception simple permettant l'insémination artificielle ou le transfert d'embryons, sous endoscopie, sans anesthésie générale, ce qui écarte les risques post-opératoires, tout en permettant d'aug­menter la fiabilité et l'efficacité de l'intervention.To overcome these drawbacks, use is made, according to the invention, of a process and an instrument of simple design allowing artificial insemination or embryo transfer, under endoscopy, without general anesthesia, which eliminates the risks post- while increasing the reliability and effectiveness of the intervention.

Le procédé selon l'invention est caractérisé en ce que l'on pratique dans la paroi abdominale de l'animal, de fines incisions rapprochées, l'une étant destinée à recevoir un endoscope permettant de visionner le champ opératoire, l'autre étant destinée au passage d'un trocart pourvu d'un manchon guide, le retrait de ce trocart, après perforation, permettant le passage, dans le manchon guide resté en place, d'un instrument d'insémination, de transplan­tation ou de prélèvement de liquides folliculaires, présentant l'originalité d'être relié à un moyen d'aspiration et de comporter par ailleurs, une seringue d'injection pour permettre d'une part, la fixation, par effet de ventouse, de la paroi d'une des deux cornes utérine et, d'autre part, de tra­verser l'endomètre pour amener l'aiguille dans la lumière de la corne à traiter, afin d'y déposer la semence ou les embryons ou encore de pratiquer les prélèvements souhaités, après quoi on traite, toujours sous endoscopie, la deuxième corne, après re­trait de l'aiguille et nouvelle orientation de l'instrument.The method according to the invention is characterized in that one makes in the abdominal wall of the animal, fine close incisions, one being intended to receive an endoscope making it possible to view the operating field, the other being intended the passage of a trocar provided with a guide sleeve, the withdrawal of this trocar, after perforation, allowing the passage, in the guide sleeve remaining in place, of an insemination, transplantation or follicular fluid removal instrument , presenting the originality of being connected to a suction means and also comprising, a injection syringe to allow on the one hand, the fixing, by suction effect, of the wall of one of the two uterine horns and, on the other hand, to cross the endometrium to bring the needle into the lumen of the horn to be treated, in order to deposit the semen or the embryos therein or to practice the desired samples, after which the second horn is treated, still under endoscopy, after withdrawal of the needle and new orientation of the instrument.

L'instrument pour la mise en oeuvre de ce procédé est constitué d'une sonde rigide formée de deux conduits coa­xiaux séparés, l'un relié à une pompe d'aspiration, l'autre servant de guide à un cathéter punctiforme dont la base est encochée radialement pour que l'opérateur puisse visionner le déplacement de l'aiguille et notamment contrôler son introduc­tion dans la lumière de la corne.The instrument for implementing this process consists of a rigid probe formed by two separate coaxial conduits, one connected to a suction pump, the other serving as a guide for a punctate catheter whose base is radially slotted so that the operator can view the movement of the needle and in particular control its introduction into the lumen of the horn.

Selon une caractéristique de l'invention le cathé­ter est constitué d'une seringue comprenant d'une part une gaine, fermée à une de ses extrémités par un embout prolongé par une aiguille, ledit embout comportant une cavité interne tronconique, d'autre part un piston coulissant avec étanchéité dans cette gaine.According to a characteristic of the invention, the catheter consists of a syringe comprising on the one hand a sheath, closed at one of its ends by an end piece extended by a needle, said end piece comprising a frustoconical internal cavity, on the other hand a sliding piston with seal in this sheath.

Selon un mode de réalisation préférentiel, le cathé­ter renferme une paillette fine de conditionnement de la semence ou des embryons, rendu solidaire de l'embout par emmanchement à force dans sa cavité tronconique, l'injection étant alors réa­lisée par le piston coulissant placé en appui, par son extrémi­té frontale, sur le bouchon obturant usuellement ce type de paillettes.According to a preferred embodiment, the catheter contains a fine straw for conditioning the semen or embryos, made integral with the tip by force fitting into its frustoconical cavity, the injection then being carried out by the sliding piston placed in support , by its front end, on the plug usually closing this type of glitter.

D'autres caractéristiques et avantages ressortiront de la description ci-après et des dessins annexés dans lesquels :

  • - la Fig. 1 est une vue schématique illustrant, en coupe, l'instrument d'insémination, de transfert d'embryons ou de ponction de liquides folliculaires, l'endoscope ayant été volontairement représenté bien que dans la pratique il serait masqué par ledit instrument,
  • - la Fig. 2 est une vue en coupe selon la ligne AA de la figure 1,
  • - la Fig.3 est une vue partielle en coupe de l'ex­trémité postérieure de l'instrument,
  • - la Fig.4 est une vue en coupe longitudinale de l'extrémité introductive de l'instrument utilisant une paillet­te fine de conditionnement.
Other characteristics and advantages will emerge from the description below and the attached drawings in which:
  • - Fig. 1 is a schematic view illustrating, in section, the insemination instrument, embryo transfer or puncture of follicular fluids, the endoscope having been voluntarily represented although in practice it would be masked by said instrument,
  • - Fig. 2 is a sectional view along line AA of figure 1,
  • - Fig.3 is a partial sectional view of the rear end of the instrument,
  • - Fig.4 is a longitudinal sectional view of the introductory end of the instrument using a fine packaging straw.

Le but essential de l'invention est de mettre à la disposition des praticiens une technologie et une instrumenta­tion souples, de conception simple, qui soient néanmoins d'une grande fiabilité et d'un coût le plus réduit possible.The essential object of the invention is to provide practitioners with flexible technology and instrumentation, of simple design, which are nevertheless of high reliability and at the lowest possible cost.

Pour ce faire, et sans récuser la chirurgie sous anesthésie générale, on fait appel ici à de la petite chirurgie non stressante pour l'animal et qui, en raison de son innocuité, peut être facilement renouvelée sans grand risque potentiel.To do this, and without rejecting surgery under general anesthesia, we use here small surgery that is not stressful for the animal and which, because of its harmlessness, can be easily renewed without great potential risk.

Conformément au procédé, on pratique sur l'abdomen de l'animal, (sensiblement au voisinage de ce que l'on dénomme la "ligne blanche" pour les ovins et caprins et sur le flanc pour les bovins et équins) deux incisions 1 et 2, fines et rap­prochées, et on introduit dans l'une d'elles, un endoscope 3 permettant de visualiser le champ opératoire. On perfore en­suite la paroi abdominale et le péritoine à l'aide d'un trocart, constitué de façon classique d'un mandrin (non représenté) et de son manchon guide 4 qui servira, après retrait du mandrin, de fourreau pour l'introduction et le guidage de l'instrument 5 spécifique de l'invention.In accordance with the process, two incisions 1 and 2 are made on the abdomen of the animal (approximately in the vicinity of what is called the "white line" for sheep and goats and on the flank for cattle and horses). 2, fine and close together, and an endoscope 3 is introduced into one of them, making it possible to visualize the operating field. The abdominal wall and the peritoneum are then punctured using a trocar, conventionally constituted by a mandrel (not shown) and its guide sleeve 4 which, after removal of the mandrel, will serve as a sheath for the introduction and guiding the specific instrument 5 of the invention.

Comme illustré en figure 1, cet instrument s'appa­rente à une sonde rigide comprenant deux canaux tangentiels 5₁, 5₂ (figure 2), ceux-ci étant définis par deux tubes 6 et 7, de diamètres différents, logés l'un dans l'autre et réunis à leurs deux extrémités par brasure, le conduit 5₁ étant séparé du conduit 5₂ et comportant une encoche radiale 5₃ de visuali­sation de l'aiguille.As illustrated in Figure 1, this instrument is similar to a rigid probe comprising two tangential channels 5₁, 5₂ (Figure 2), these being defined by two tubes 6 and 7, of different diameters, housed one in the other and joined at their two ends by brazing, the conduit 5₁ being separated from the conduit 5₂ and comprising a radial notch 5₃ for viewing the needle.

Le canal extérieur 5₂ est relié par raccord 8 à un moyen d'aspiration, tandis que le canal intérieur 5₁ sert de guide à un cathéter punctiforme 9 formé essentiellement (fi­gure 4) d'une gaine 10, dont la base est obturée par un embout 11 prolongé par une aiguille 12, cette gaine introduite dans le canal 5₁ recevant un piston 13, étalonné à l'aide de re­pères 13₁, dont la tête coulisse avec étanchéité dans le con­duit intérieur de la gaine.The external channel 5₂ is connected by connection 8 to a suction means, while the internal channel 5₁ serves as a guide for a punctiform catheter 9 formed essentially (FIG. 4) of a sheath 10, the base of which is closed by a nozzle. 11 extended by a needle 12, this sheath introduced into the channel 5₁ receiving a piston 13, calibrated using marks 13 de, the head of which slides with sealing in the inner duct of the sheath.

Dans la version la plus simple, en particulier lorsque l'on procède à l'injection de semences fraiches ou à la transplantation immédiate d'embryons, le cathéter est constitué, comme décrit précédemment, d'une simple gaine à aiguille et d'un piston d'injection. Lorsque l'on fait appel à de la semence ou des embryons qui ont été congelés, on a alors recours à des paillettes fines 14, que l'on fixe à l'in­térieur du cathéter, et dont le bouchon d'obturation 15 sert de piston. On sait en effet que ces paillettes fines servent à la conservation, en milieu cryogénique, des semences ou em­bryons et qu'elles sont formées d'un tube capillaire soudé à une de ses extrémités et fermé à l'autre par un bouchon con­tenant une poudre polymérisable au contact de la substance. Lorsque l'on utilise ici de telles paillettes on doit section­ner l'extrémité antérieure et l'introduire à force dans la cavité conique 16 de l'embout du cathéter afin de l'immobili­ser. Il suffit ensuite d'utiliser le piston 13, après avoir retiré sa tête amovible, afin que celui-ci puisse coulisser dans la paillette fine et venir en appui frontal sur le bou­chon 15 qui joue alors de rôle de tête de piston.In the simplest version, in particular when injecting fresh seeds or immediately transplanting embryos, the catheter consists, as described above, of a simple needle sheath and a injection piston. When semen or embryos that have been frozen are used, then fine flakes 14 are used, which are fixed inside the catheter, and the closure plug 15 of which serves as a piston. We know that these fine flakes are used for the conservation, in cryogenic medium, of seeds or embryos and that they are formed of a capillary tube welded at one of its ends and closed at the other by a plug containing a powder. polymerizable on contact with the substance. When such flakes are used here, the anterior end must be cut off and forced into the conical cavity 16 of the catheter tip in order to immobilize it. It then suffices to use the piston 13, after having removed its removable head, so that the latter can slide in the fine straw and come into abutment on the plug 15 which then acts as the piston head.

Le fonctionnement de cet instrument est simple puisque lorsque la sonde 5 est amenée en position, sous en­doscopie, en regard d'une des deux cornes utérines, on ouvre le robinet 17, prévu sur le raccord 8, et l'on crée une dé­pression dans le canal 5₂ de manière à fixer par effet de ventouse, la paroi de la corne qui, comme on le sait est fu­yante et a, par conséquent, tendance à se dérober. Lorsque la corne est ainsi immobilisée par succion, on descend le cathéter et l'on contrôle l'introduction de l'aiguille 12 au travers de l'endomètre 18 jusqu'à ce que son extrémité vienne se situer dans la lumière 19 de la corne considérée. A ce stade le cathéter, qui comporte à son extrémité arrière une collerette 21 vient en butée sur la face postérieure 22 de l'instrument de manière à ce que l'aiguille ne traverse pas la paroi opposée de la corne. Dans cette position on procède à la descente du piston 13 qui expulse dans ladite cavité 19 la semence ou les embryons contenus dans la gaine 10 ou dans la paillette 14.The operation of this instrument is simple since when the probe 5 is brought into position, under endoscopy, facing one of the two uterine horns, the tap 17, provided on the connector 8, is opened and a vacuum is created in the channel 5₂ so as to fix by suction effect, the wall of the horn which, as is known is leaky and therefore tends to slip away. When the horn is thus immobilized by suction, the catheter is lowered and the introduction of the needle 12 is controlled through the endometrium 18 until its end comes to be located in the lumen 19 of the horn considered. At this stage the catheter, which has at its rear end a flange 21 abuts on the rear face 22 of the instrument so that the needle does not pass through the opposite wall of the horn. In this position, the piston 13 is lowered, which expels into said cavity 19 the semen or the embryos contained in the sheath 10 or in the straw 14.

En matière d'insémination d'ovins et caprins, le cathéter renferme deux doses de quinze millions de spermato­zoïdes chacune pour traiter les deux cornes 20. Lorsqu'une dose a été injectée, il suffit de procéder au retrait partiel du cathéter et en particulier de l'aiguille 12, puis d'incliner, sous endoscopie, l'instrument, pour amener son extrémité fron­tale en regard de la deuxième corne, puis de renouveler les opérations.In terms of insemination of sheep and goats, the catheter contains two doses of fifteen million sperm each to treat the two horns 20. When a dose has been injected, it suffices to partially withdraw the catheter and in particular to needle 12, then tilt, under endoscopy, the instrument, to bring its front end facing the second horn, then repeat the operations.

Etant donné que l'on doit, préalablement à l'inter­vention, repousser les viscères de la paroi abdominale, afin d'avoir un champ optique maximum et une bonne visualisation de l'appareil génital, il est souhaitable de prévoir à l'extrémité postérieure de l'instrument (figure 3) un presse-étoupe 23 à joint torique 24 permettant d'obturer efficacement le canal extérieur 5₂ afin d'éviter une remontée de l'air à travers ce conduit.Since the viscera of the abdominal wall must be pushed back before the operation, in order to have a maximum optical field and a good visualization of the genital tract, it is desirable to provide at the posterior end of the instrument (FIG. 3) a cable gland 23 with an O-ring 24 making it possible to effectively seal the external channel 5₂ in order to avoid a rise of the air through this conduit.

Le procédé et l'instrument selon l'invention présentent de nombreux avantages, car à la finesse de la chirurgie utilisée et son innocuité, viennent s'ajouter ceux liés à la souplesse et au caractère économique de l'intervention. En effet, l'instrument de conception simple permet de traiter les animaux directement sur le terrain, puisqu'on n'a plus recours aux installations propres à la chirurgie lourde et à l'anesthésie générale. En outre, les incisions étant fines, l'animal est peu stressé et les soins post-opératoires prati­quement inexistants. Enfin, et il s'agit là d'un avantage particulièrement important dans le cadre de l'insemination artificielle des ovins et caprins, on peut mettre en place à l'aide de cette instrumentation, un faible nombre de sperma­tozoïdes (trente millions pour les deux cornes) là où il fal­lait, avec les techniques traditionnelles 400 millions de spermato­zoïdes. Par conséquent, le rendement est élevé, l'injection de semences, directement dans l'utérus, améliorant à la fois le taux de fertilité et le pouvoir de diffusion des mâles. On doit souligner en outre, que cet instrument est particulièrement bien adapté au transfert embryonnaire et aux prélèvements de liquides folliculaires chez les ovins, les caprins, les bovins ou les équins, seules les dimensions de l'appareil changeant en fonction de la taille de l'animal. En outre, cet instrument est d'une manipu­lation aisée car l'opérateur peut d'une seule main tenir l'en­doscope et la sonde qui comporte à cet effet, un anneau de préhension 25.The method and the instrument according to the invention have many advantages, because to the finesse of the surgery used and its safety, are added those linked to the flexibility and the economic nature of the intervention. Indeed, the simple design instrument makes it possible to treat animals directly in the field, since we no longer have recourse to the facilities specific to heavy surgery and general anesthesia. In addition, the incisions being fine, the animal is not very stressed and the post-operative care practically non-existent. Finally, and this is a particularly important advantage in the context of artificial insemination of sheep and goats, we can set up using this instrumentation, a low number of spermatozoa (thirty million for the two horns) where it was needed, with traditional techniques 400 million spermatozoa. Consequently, the yield is high, the injection of seeds directly into the uterus, improving both the fertility rate and the diffusion power of the males. It should also be emphasized that this instrument is particularly well suited to embryo transfer and the removal of follicular fluids from sheep, goats, cattle or horses, only the dimensions of the device changing according to the size of the body. 'animal. In addition, this instrument is easy to handle because the operator can hold the endoscope and the probe with one hand, which includes a gripping ring 25 for this purpose.

Claims (6)

1°. Instrument pour l'insémination artifi­cielle, la transplantation d'embryons in-utéro ou le prélèvement de liquide folliculaire par voie transpé­ritonéale chez les animaux, notamment sur les ovins, caprins, bovins ou équins, caractérisé en ce qu'il est constitué d'une sonde rigide (5) comprenant deux ca­naux tangentiels (5₁,5₂) définis par deux conduits de diamètres différents, logés l'un dans l'autre, l'un des conduits (5₂) étant relié à une pompe d'aspiration l'autre (5₁) servant de guide à un cathéter punctifor­me (10-13), la base de ce dernier étant encochée ra­dialement pour permettre de visionner, sous endosco­pie, le déplacement de l'aiguille (12).1 °. Instrument for artificial insemination, transplantation of intrauterine embryos or removal of follicular fluid by transperitoneal route from animals, in particular from sheep, goats, cattle or horses, characterized in that it consists of a rigid probe (5) comprising two tangential channels (5₁, 5₂) defined by two conduits of different diameters, housed one inside the other, one of the conduits (5₂) being connected to a suction pump the other (5₁) serving as a guide for a punctate catheter (10-13), the base of the latter being radially slotted to allow viewing, under endoscopy, the movement of the needle (12). 2°. Instrument selon la revendication 1, caractérisé en ce que le cathéter est constitué d'une part, d'une gaine semi-rigide (10) fermée à une de ses extrémités par un embout (11) prolongé par une aiguille (12), ledit embout comportant une cavité interne tronconique (16), d'autre part un piston (13) coulissant avec étanchéité dans cette gaine.2 °. Instrument according to claim 1, characterized in that the catheter consists on the one hand, of a semi-rigid sheath (10) closed at one of its ends by a nozzle (11) extended by a needle (12), said end piece comprising a frustoconical internal cavity (16), on the other hand a piston (13) sliding with sealing in this sheath. 3°. Instrument selon la revendication 2, caractérisé en ce que le cathéter renferme une pail­lette fine (14) de conditionnement de la semence, des embryons ou des liquides folliculaires, rendue soli­daire de l'embout (11) par emmanchement à force dans sa cavité tronconique (16), l'injection ou la ponc­tion étant réalisée par le piston coulissant (13) en appui frontal sur le bouchon (15), obturant usuel­lement ces paillettes.3 °. Instrument according to claim 2, characterized in that the catheter contains a fine straw (14) for conditioning the semen, embryos or follicular fluids, made integral with the tip (11) by force fitting into its frustoconical cavity ( 16), the injection or the puncture being carried out by the sliding piston (13) in frontal abutment on the plug (15), usually closing these flakes. 4°. Instrument selon l'une quelconque des revendications de 1 à 3, caractérisé en ce que l'extré­mité supérieure de la sonde est obturée hermétiquement par un presse-étoupe (23), traversé par la gaine (10) de la seringue.4 °. Instrument according to any one of claims 1 to 3, characterized in that the upper end of the probe is hermetically sealed by a cable gland (23), crossed by the sheath (10) of the syringe. 5°. Instrument selon l'une quelconque des revendications de 1 à 4, caractérisé en ce que le piston est étalonné.5 °. Instrument according to any one of Claims 1 to 4, characterized in that the piston is calibrated. 6°. Procédé pour la mise en place et la mise en oeuvre de l'instrument selon les revendications de 1 à 5, caractérisé en ce que l'on pratique deux fines incisions dans la paroi abdominale de l'animal, on introduit dans l'une un endoscope pour visionner le champ opératoire et dans l'autre un trocart pourvu de son manchon guide, puis on procède au retrait du tro­cart et on engage dans le manchon resté en place l'ins­trument d'insémination et, de transplantation ou de prélèvement, relié à un moyen d'aspiration et compor­tant une seringue, on fixe ensuite par effet de ven­touse la paroi d'une des cornes utérines puis on tra­verse l'endomètre pour amener l'aiguille dans la lumière de cette corne afin de déposer la semence ou les em­bryons où diffère le prélèvement souhaité, après quoi on traite, toujours sous endoscopie, la deuxième corne dans les mêmes conditions, après retrait de l'aiguille et nouvelle orientation de l'instrument.6 °. Method for the installation and the implementation of the instrument according to Claims 1 to 5, characterized in that two fine incisions are made in the abdominal wall of the animal, one introduces into one endoscope to view the operating field and in the other a trocar provided with its guide sleeve, then we proceed to the removal of the trocar and we engage in the sleeve remained in place the insemination instrument and, transplant or sample, connected to a suction means and comprising a syringe, the wall of one of the uterine horns is then fixed by suction effect, then the endometrium is crossed to bring the needle into the lumen of this horn in order to deposit the semen or the embryos where the desired sample differs, after which the second horn is treated, still under endoscopy, under the same conditions, after removal of the needle and new orientation of the instrument.
EP86401825A 1985-08-19 1986-08-18 Instrument and process for artificial insemination, in utero embryo transplants or taking follicular liquid by transperitoneal means in animals Withdrawn EP0214043A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8512491 1985-08-19
FR8512491A FR2597328A1 (en) 1985-08-19 1985-08-19 INSTRUMENT FOR ARTIFICIAL INSEMINATION, TRANSPLANTATION OF EMBRYOS IN UTERO OR SAMPLING OF FOLLICULAR LIQUIDS, BY TRANSPERITONEAL ROUTE, IN ANIMALS.

Publications (1)

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EP0214043A1 true EP0214043A1 (en) 1987-03-11

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EP86401825A Withdrawn EP0214043A1 (en) 1985-08-19 1986-08-18 Instrument and process for artificial insemination, in utero embryo transplants or taking follicular liquid by transperitoneal means in animals

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EP (1) EP0214043A1 (en)
AU (1) AU6154286A (en)
BR (1) BR8603951A (en)
ES (1) ES2001100A6 (en)
FR (1) FR2597328A1 (en)
GR (1) GR862140B (en)
ZA (1) ZA866057B (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2603796A1 (en) * 1986-09-17 1988-03-18 Agronomique Inst Nat Rech INSTRUMENTATION FOR THE COLLECTION OF EMBRYOS, UNDER LAPAROSCOPY, IN SMALL RUMINANTS
FR2609885A1 (en) * 1987-01-22 1988-07-29 Cassou Robert INSTRUMENT FOR ARTIFICIAL INSEMINATION, TRANSFER OF EMBRYOS OR SAMPLING OF FOLLICULAR LIQUIDS IN MAMMALS
US6117068A (en) * 1995-10-19 2000-09-12 Elite Genetics, Inc Artificial insemination system
ES2156579A1 (en) * 2000-01-20 2001-06-16 Univ Murcia Device and method for introducing and/or collecting fluids from the interior of the uterus of an animal
WO2002028311A1 (en) * 2000-10-05 2002-04-11 Xy, Inc. System of hysteroscopic insemination of mares
EP2329793A2 (en) 2009-12-02 2011-06-08 German Genetics International GmbH Catheter for artificial insemination
EP2353543A1 (en) * 2008-09-26 2011-08-10 Universidad de Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
EP2529695A2 (en) 2011-05-31 2012-12-05 German Genetics International GmbH Endoscope for gamete transfer
CN104546219A (en) * 2015-01-22 2015-04-29 山东东阿阿胶股份有限公司 Semen deposition gun for donkey and application method thereof
US9433484B2 (en) 2007-07-27 2016-09-06 Brad K. Stroud Artificial breeding techniques for bovines including semen diluents and AI apparatus
US9554883B2 (en) 2010-08-10 2017-01-31 Brad K. Stroud Method and apparatus to reduce the number of sperm used in artificial insemination of cattle
US10610343B2 (en) 2013-07-03 2020-04-07 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine
US11622844B2 (en) 2010-08-10 2023-04-11 Maximate, Llc Method, apparatus and kit for artificial insemination of bovine

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BRPI0905581B1 (en) * 2009-12-17 2021-03-23 Empresa Brasileira De Pesquisa Agropecuária - Embrapa CIRCUIT FOR EMBRYO COLLECTION THROUGH THE TRANSCERVICAL VIA IN CAPRINE AND SHEEP
BRPI1004431A2 (en) * 2010-06-09 2012-02-14 Embrapa probe for transcervical embryo collection in goats and sheep
FR3036954B1 (en) * 2015-06-03 2017-07-14 Imv Tech STRAW FOR PRESERVING A PREDETERMINED DOSE OF LIQUID-BASED SUBSTANCE, AND INJECTION DEVICE AND METHOD FOR CARRYING OUT THE SAME

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US3792703A (en) * 1972-07-10 1974-02-19 Deseret Pharma Catheter placement unit
WO1982001825A1 (en) * 1980-12-03 1982-06-10 Kevin W Gobby Apparatus for artificial insemination
WO1985000009A1 (en) * 1983-06-15 1985-01-03 Wiegerinck Martinus Antonius H Method and device to perforate, in particular to puncture a membranous member while utilizing vacuum fixation

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US1422490A (en) * 1921-03-26 1922-07-11 Stader Otto Veterinary surgical instrument
US3792703A (en) * 1972-07-10 1974-02-19 Deseret Pharma Catheter placement unit
WO1982001825A1 (en) * 1980-12-03 1982-06-10 Kevin W Gobby Apparatus for artificial insemination
WO1985000009A1 (en) * 1983-06-15 1985-01-03 Wiegerinck Martinus Antonius H Method and device to perforate, in particular to puncture a membranous member while utilizing vacuum fixation

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2603796A1 (en) * 1986-09-17 1988-03-18 Agronomique Inst Nat Rech INSTRUMENTATION FOR THE COLLECTION OF EMBRYOS, UNDER LAPAROSCOPY, IN SMALL RUMINANTS
FR2609885A1 (en) * 1987-01-22 1988-07-29 Cassou Robert INSTRUMENT FOR ARTIFICIAL INSEMINATION, TRANSFER OF EMBRYOS OR SAMPLING OF FOLLICULAR LIQUIDS IN MAMMALS
EP0278823A1 (en) * 1987-01-22 1988-08-17 Robert Cassou Instrument for artificial insemination, embryo transfer, or sampling of folicular fluids
US4846785A (en) * 1987-01-22 1989-07-11 Robert Cassou Instrument for artificial insemination, embryo transfer or sampling follicular liquids in mammals
US6117068A (en) * 1995-10-19 2000-09-12 Elite Genetics, Inc Artificial insemination system
ES2156579A1 (en) * 2000-01-20 2001-06-16 Univ Murcia Device and method for introducing and/or collecting fluids from the interior of the uterus of an animal
WO2001052767A1 (en) * 2000-01-20 2001-07-26 Universidad De Murcia Device and method for introducing and/or collecting fluids from he interior of the uterus of an animal
AU776612B2 (en) * 2000-01-20 2004-09-16 Universidad De Murcia A device and method for introducing and/or collecting fluids in the inside of an animal uterus
WO2002028311A1 (en) * 2000-10-05 2002-04-11 Xy, Inc. System of hysteroscopic insemination of mares
GB2383543A (en) * 2000-10-05 2003-07-02 Xy Inc System of hysteroscopic insemination of mares
GB2383543B (en) * 2000-10-05 2005-03-09 Xy Inc System of hysteroscopic insemination of mares
US9433484B2 (en) 2007-07-27 2016-09-06 Brad K. Stroud Artificial breeding techniques for bovines including semen diluents and AI apparatus
AU2009295755B2 (en) * 2008-09-26 2015-07-16 Universidad De Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
US9277981B2 (en) 2008-09-26 2016-03-08 Universidad De Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
EP3263067A1 (en) * 2008-09-26 2018-01-03 Universidad de Murcia Device for inserting in or obtaining a fluid with gametes, embryos or any other type of solution from the oviduct of a sow
EP2353543A1 (en) * 2008-09-26 2011-08-10 Universidad de Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
EP2353543A4 (en) * 2008-09-26 2014-04-09 Univ Murcia Device and method for inserting or obtaining a fluid with gametes, embryos or any other type of solution in the oviduct of a sow
DE102009047423A1 (en) 2009-12-02 2011-06-09 German Genetics International Gmbh catheter device
EP2329793A2 (en) 2009-12-02 2011-06-08 German Genetics International GmbH Catheter for artificial insemination
US9554883B2 (en) 2010-08-10 2017-01-31 Brad K. Stroud Method and apparatus to reduce the number of sperm used in artificial insemination of cattle
USRE48283E1 (en) 2010-08-10 2020-10-27 Brad Stroud Method and apparatus to reduce the number of sperm used in artificial insemination of cattle
US11622844B2 (en) 2010-08-10 2023-04-11 Maximate, Llc Method, apparatus and kit for artificial insemination of bovine
DE102011076844A1 (en) 2011-05-31 2012-12-06 German Genetics International Gmbh Endoscope for gamete transfer
EP2529695A2 (en) 2011-05-31 2012-12-05 German Genetics International GmbH Endoscope for gamete transfer
US10610343B2 (en) 2013-07-03 2020-04-07 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine
CN104546219A (en) * 2015-01-22 2015-04-29 山东东阿阿胶股份有限公司 Semen deposition gun for donkey and application method thereof

Also Published As

Publication number Publication date
ZA866057B (en) 1987-03-25
ES2001100A6 (en) 1988-04-16
BR8603951A (en) 1987-03-24
AU6154286A (en) 1987-02-26
GR862140B (en) 1986-12-30
FR2597328A1 (en) 1987-10-23

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