EP0133756A3 - Vektor für die Expression von Polypeptiden - Google Patents
Vektor für die Expression von Polypeptiden Download PDFInfo
- Publication number
- EP0133756A3 EP0133756A3 EP84304662A EP84304662A EP0133756A3 EP 0133756 A3 EP0133756 A3 EP 0133756A3 EP 84304662 A EP84304662 A EP 84304662A EP 84304662 A EP84304662 A EP 84304662A EP 0133756 A3 EP0133756 A3 EP 0133756A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- expression
- proteases
- vector
- polypeptides
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
- C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US51119883A | 1983-07-06 | 1983-07-06 | |
US511198 | 1983-07-06 | ||
US61890284A | 1984-06-08 | 1984-06-08 | |
US618902 | 1984-06-08 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0133756A2 EP0133756A2 (de) | 1985-03-06 |
EP0133756A3 true EP0133756A3 (de) | 1986-10-01 |
Family
ID=27057152
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP84304662A Withdrawn EP0133756A3 (de) | 1983-07-06 | 1984-07-06 | Vektor für die Expression von Polypeptiden |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0133756A3 (de) |
CA (1) | CA1311429C (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0149241B1 (de) * | 1983-12-28 | 1991-03-27 | Agency Of Industrial Science And Technology | DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS |
JP2500311B2 (ja) * | 1985-03-19 | 1996-05-29 | 工業技術院長 | 蛋白製造法 |
US4769327A (en) * | 1985-03-29 | 1988-09-06 | Biotechnica International, Inc. | Secretion vector |
IT1186750B (it) * | 1985-07-10 | 1987-12-16 | Eniricerche Spa | Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni |
US5017477A (en) * | 1985-10-25 | 1991-05-21 | Biotechnica International, Inc. | Enhancing DNA sequencies derived from the sacQ gene |
JPH0669377B2 (ja) * | 1985-11-25 | 1994-09-07 | 工業技術院長 | Dna塩基配列およびベクタ− |
FI870103A (fi) * | 1986-01-13 | 1987-07-14 | Genex Corp | Producering av humanserum albumin i en bacillus-stam. |
JPS6387975A (ja) * | 1986-10-02 | 1988-04-19 | Agency Of Ind Science & Technol | 枯草菌菌株 |
DE3711883A1 (de) * | 1987-04-08 | 1988-10-27 | Merck Patent Gmbh | Genetische kontrolleinheit und klonierungs- und expressionssystem |
US5162207A (en) * | 1989-07-07 | 1992-11-10 | E. I. Du Pont De Nemours And Company | Sucrose inducible expression vectors for bacillus sp. |
CA2069147A1 (en) * | 1991-05-22 | 1992-11-23 | Kazuaki Kitano | Dna and its use |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2091268A (en) * | 1980-12-31 | 1982-07-28 | Palva Ilkka | Recombinant-DNA-molecules and methods for protein production |
EP0077109A2 (de) * | 1981-10-14 | 1983-04-20 | Unilever N.V. | DNS Moleküle enthaltend die Gene für Preprochymosin und dessen Reifungsformen und dadurch transformierte Mikroorganismen |
WO1984000773A1 (en) * | 1982-08-23 | 1984-03-01 | Pharmacia Ab | Recombinant dna molecule, transformed microorganism and process for producing staphylococcal protein a |
EP0134048A1 (de) * | 1983-07-06 | 1985-03-13 | Gist-Brocades N.V. | Molekulare Klonierung und Expression in industriellen Mikroorganismen |
EP0149241A2 (de) * | 1983-12-28 | 1985-07-24 | Agency Of Industrial Science And Technology | DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS |
EP0151760A1 (de) * | 1984-01-06 | 1985-08-21 | Takeda Chemical Industries, Ltd. | DNA und ihre Verwendung |
EP0130756B1 (de) * | 1983-06-24 | 1991-02-06 | Genencor International, Inc. | Prokaryotische Carbonyl-Hydrolasen, Verfahren, DNA, Vektoren und transformierte Wirte zu ihrer Herstellung und diese Hydrolasen enthaltende Detergenszusammensetzungen |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS58162291A (ja) * | 1982-03-23 | 1983-09-26 | Res Dev Corp Of Japan | 耐熱性プロテア−ゼの製造法 |
JPS5959190A (ja) * | 1982-09-29 | 1984-04-04 | Agency Of Ind Science & Technol | プロテア−ゼの製造法 |
JPH0659224B2 (ja) * | 1983-09-12 | 1994-08-10 | 工業技術院長 | Dνa塩基配列及びそのdνa塩基配列を含む組換え体プラスミドの創製法 |
JPS60141291A (ja) * | 1983-12-28 | 1985-07-26 | Agency Of Ind Science & Technol | 中性プロテア−ゼの構造遺伝子を含むdνaフラグメントおよび中性プロテア−ゼ製造法 |
-
1984
- 1984-06-28 CA CA000457677A patent/CA1311429C/en not_active Expired - Lifetime
- 1984-07-06 EP EP84304662A patent/EP0133756A3/de not_active Withdrawn
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2091268A (en) * | 1980-12-31 | 1982-07-28 | Palva Ilkka | Recombinant-DNA-molecules and methods for protein production |
EP0077109A2 (de) * | 1981-10-14 | 1983-04-20 | Unilever N.V. | DNS Moleküle enthaltend die Gene für Preprochymosin und dessen Reifungsformen und dadurch transformierte Mikroorganismen |
WO1984000773A1 (en) * | 1982-08-23 | 1984-03-01 | Pharmacia Ab | Recombinant dna molecule, transformed microorganism and process for producing staphylococcal protein a |
EP0130756B1 (de) * | 1983-06-24 | 1991-02-06 | Genencor International, Inc. | Prokaryotische Carbonyl-Hydrolasen, Verfahren, DNA, Vektoren und transformierte Wirte zu ihrer Herstellung und diese Hydrolasen enthaltende Detergenszusammensetzungen |
EP0134048A1 (de) * | 1983-07-06 | 1985-03-13 | Gist-Brocades N.V. | Molekulare Klonierung und Expression in industriellen Mikroorganismen |
EP0149241A2 (de) * | 1983-12-28 | 1985-07-24 | Agency Of Industrial Science And Technology | DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS |
EP0151760A1 (de) * | 1984-01-06 | 1985-08-21 | Takeda Chemical Industries, Ltd. | DNA und ihre Verwendung |
Non-Patent Citations (11)
Title |
---|
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, vol. 46, no. 1, January 1982, pages 65-74, Tokyo, JP; I. HUSSAIN et al.: "Clones with new phenotypes through transformation of Bacillus brevis 47, a protein-producing bacterium" * |
CHEMICAL ABSTRACTS, vol. 100, no. 5, January 1984, page 352, no. 33279j, Columbus, Ohio, US; & JP - A - 58 162 291 (RESEARCH DEVELOPMENT CORP. OF JAPAN) 26-09-1983 * |
CHEMICAL ABSTRACTS, vol. 102, no. 23, 10th June 1985, page 16, abstract no. 198918w, Columbus, Ohio, US; N. VASANTHA et al.: "Cloning of a serine protease gene from Bacillus amyloliquefaciens and its expression in Bacillus subtilis", GENET. BIOTECHNOL. BACILLI, [PROC. INT. CONF.], 2nd 1983 (Pub. 1984), 163-72 * |
CHEMICAL ABSTRACTS, vol. 102, no. 23, 10th June 1985, page 16, abstract no. 198919x, Columbus, Ohio, US; J.A. WELLS et al.: "Cloning and sequencing of a region controlling efficient expression of subtilisin from Bacillus amyloliquefaciens", & GENET. BIOTECHNOL. BACILLI, [PROC. INT. CONF.], 2nd 1983 (Pub. 1984), 173-80 * |
CHEMICAL ABSTRACTS, vol. 102, no. 3, 21st January 1985, page 152, abstract no. 18497b, Columbus, Ohio, US; N. VASANTHA et al.: "Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein", & J. BACTERIOL. 1984, 159(3), 811-19 * |
CHEMICAL ABSTRACTS, vol. 103, no. 5, August 1985, page 116, abstract no. 33022g, Columbus, Ohio, US; & JP - A - 60 58 076 (AGENCY OF INDUSTRIAL SCIENCES AND TECHNOLOGY) 04-04-1985 * |
JOURNAL OF BACTERIOLOGY, vol. 154, no. 2, May 1983, pages 831-837, American Society for Microbiology; M. FUJII et al.: "Molecular cloning of a thermostable neutral protease gene from Bacillus stearothermophilus in a vector plasmid and its expression in Bacillus stearothermophilus and Bacillus subtilis" * |
NUCLEIC ACIDS RESEARCH, vol. 11, no. 22, November 1983, pages 7911-7925, IRL Press Ltd., Oxford, GB; J.A. WELLS et al.: "Cloning, sequencing, and secretion of Bacillus amyloliquefaciens subtilisin in Bacillus subtilis" * |
PATENTS ABSTRACTS OF JAPAN, vol. 8, no. 156 (C-234)[1593], 19th July 1984; & JP - A - 59 59 190 (KOGYO GIJUTSUIN [JAPAN]) 04-04-1984 * |
PATENTS ABSTRACTS OF JAPAN, vol. 9, no. 301 (C-316)[2024], 28th November 1985; & JP - A - 60 141 291 (KOGYO GIJUTSUIN [JAPAN]) 26-07-1985 * |
PROC. NATL. ACAD. SCI. USA, vol. 80, February 1983, pages 697-701; S. LÖFDAHL et al.: "Gene for staphylococcal protein A" * |
Also Published As
Publication number | Publication date |
---|---|
EP0133756A2 (de) | 1985-03-06 |
CA1311429C (en) | 1992-12-15 |
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Legal Events
Date | Code | Title | Description |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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AK | Designated contracting states |
Designated state(s): AT BE CH DE FR GB IT LI LU NL SE |
|
PUAL | Search report despatched |
Free format text: ORIGINAL CODE: 0009013 |
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AK | Designated contracting states |
Kind code of ref document: A3 Designated state(s): AT BE CH DE FR GB IT LI LU NL SE |
|
17P | Request for examination filed |
Effective date: 19870113 |
|
17Q | First examination report despatched |
Effective date: 19890724 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 19910511 |
|
RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: NAGARAJAN, VASANTHA Inventor name: BANNER, CARL D. B. Inventor name: RHODES, CRAIG STUART |