EP0078724A1 - Präzisionspipette mit Verdrängungswirkung - Google Patents

Präzisionspipette mit Verdrängungswirkung Download PDF

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Publication number
EP0078724A1
EP0078724A1 EP82401913A EP82401913A EP0078724A1 EP 0078724 A1 EP0078724 A1 EP 0078724A1 EP 82401913 A EP82401913 A EP 82401913A EP 82401913 A EP82401913 A EP 82401913A EP 0078724 A1 EP0078724 A1 EP 0078724A1
Authority
EP
European Patent Office
Prior art keywords
piston
pipette
flange
capillary tube
clamping member
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP82401913A
Other languages
English (en)
French (fr)
Other versions
EP0078724B1 (de
Inventor
Eric Marteau D'autry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT82401913T priority Critical patent/ATE14994T1/de
Publication of EP0078724A1 publication Critical patent/EP0078724A1/de
Application granted granted Critical
Publication of EP0078724B1 publication Critical patent/EP0078724B1/de
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the present invention relates to an improvement made to precision positive displacement pipettes intended for the sampling and distribution of small quantities of liquid.
  • such positive displacement precision pipettes are already known which are of the type comprising an essentially tubular pipette body at the lower end of which is arranged a calibrated capillary tube in which it moves in a sealed and against an upward elastic restoring force exerted by a main spring, a piston controlled in its downward movement by a control rod protruding from the upper end of said pipette body, as well as a clamping member of the upper end of the piston, which is of the type with elastic branches deviating radially by displacement against the force exerted by a second spring of resistance much greater than that of the main spring and which is integral in translation with said rod control.
  • Such a positive displacement pipette is for example described in French patent application n ° 79,01335 belonging to the applicant.
  • the present invention specifically aims to achieve a positive displacement pipette which overcomes the aforementioned drawbacks.
  • the positive displacement precision pipette is characterized in that its piston has, in the vicinity of its upper end intended to be grasped by said gripping member, a collar capable of cooperating by its upper face with the 'free end of the elastic branches of the clamping member and by its underside with an abutment surface formed on the inner surface of said capillary tube tip.
  • the positive displacement pipette comprises an essentially tubular body 10 which, in a particular embodiment may be constituted by two separate parts secured to each other by screwing.
  • the upper part or housing (not shown) allows for example to incorporate a mechanism for adjusting and displaying the volume to be withdrawn and distributed, and the tubular lower part 12 is used mainly for guiding the nipping member 14 of the piston 16.
  • the particular adjustment and display mechanism is not part of the present invention and will therefore not be described here in detail. Such a mechanism is for example described in detail in French patent application No. 80 00130 filed in the name of the applicant and the lessons of which are integrated into the present description.
  • a calibrated capillary tube 18 in which moves, in a sealed manner and against an upward elastic restoring force exerted by a main spring 20, a piston 16 controlled in its movement by a control rod 22 projecting from the upper end of the case of the pipette body 10.
  • the pipette has a tip 24 which ends in a calibrated capillary tube 18 and which is able to cooperate by fitting with the free lower end of the pipette body 10.
  • a tip 24 will be made of a thermoplastic material chosen to resist the samples with which it comes into contact. It will for example be made of polypropylene or any other semi-rigid thermoplastic material.
  • the piston 16 has at least one sealing zone 26 coming into intimate contact with the inner surface of the capillary tube 18.
  • a piston 16 can for example advantageously be produced in the form of a 'a stainless steel wire having a coating, for example a coating of polyethylene or teflon, at the sealing zone 26.
  • a coating can for example affect a shape generally cylindrical or else may be equipped at its periphery with at least one annular sealing lip. coming into intimate contact with the internal surface of the capillary tube 18.
  • Such a type of pipette also comprises a clamping member 14 of the upper end of the piston 16, which is of the type with elastic branches 28 which move radially apart by displacement against the force exerted by a second spring (not shown). ) of resistance much higher than that of the main spring 20. It is clear that such a clamping member 14 must be secured in translation with the control rod 22 of the pipette.
  • this pinching member 14 can for example be in the form of a self-contained cartridge comprising a clamp proper ending at its lower end by elastic branches 28 and the other end of which is screwed into a clamp holder.
  • This clamp support is suitable for slide, against the force exerted by the second spring and according to a partial stroke in the upper part of an orifice formed in the clamp holder 30.
  • the latter clamp holder 30 slides itself inside the lower part 12 of the pipette body 10 against the force exerted by the main spring 20. It is important that the end of the elastic branches 28 has an outwardly flaring surface capable of being applied closely against the lower conical opening 32 of the clamp holder 30, and this precisely under the action of the force exerted by the second spring.
  • the assembly of this cartridge which constitutes the pinching member of the piston 16 is elastically urged upwards under the action of the force exerted by the main spring 20.
  • the holder clamp 30 has a shoulder 34 against which the main spring rests. By its lower end, the main spring 20 comes into fixed support on a shoulder 36 formed on the inner surface of the lower part 12 of the pipette body 10 , in the vicinity of its free lower end.
  • the clamp holder 30 can advantageously come into upper abutment against an adjustment screw (not shown), the variable vertical position of which makes it possible to adjust the useful stroke for taking and dispensing the liquid sample.
  • the present invention is however not limited to such a type of adjustable pipette, but can of course also extend to fixed pipettes, ie to pipettes whose adjusting screw would be replaced by a stroke limiting stopper. high which remains fixed in position relative to the pipette body 10.
  • the limiting stop down the useful stroke for dispensing the pipette is made at a shoulder 38 formed in the vicinity of the lower part of the clamp holder 30.
  • This shoulder 38 is able to cooperate with the shoulder corresponding 36 formed in the vicinity of the lower end of the pipette body 10.
  • Such a lower limit position of the clamp-holder member 14 is illustrated in FIG. 2. It will be noted that this position is obtained at the end of the compression complete with main spring 20.
  • the orifice 40 of the free lower end of the pipette body 10 is arranged so as to allow at least one part of the clamping member 14 to pass, which, after compression of the second spring and at the end of its travel towards the bottom, ensures the simultaneous ejection of said end piece 24 and of the piston 16 which remains trapped in the capillary tube 18.
  • the piston 16 has a flange 42 located near its upper end intended to be gripped by the elastic branches 28 of the pinching member 14.
  • This flange 42 has an upper face able to cooperate with the free end of the elastic branches 28 of the pinching member 14, as well as a lower face able to cooperate with an abutment surface 44 formed on the inner wall of the cap 24 with capillary tube 18, preferably in the vicinity of the junction between the upper flared part of the cap and the capillary tube 18 proper.
  • the flange 42 has a transverse dimension substantially equal to the outside diameter of the elastic branches 28, in the separated state, of the clamping member 14.
  • This flange 42 has on its underside at least one peripheral annular zone defining an abutment surface which is substantially perpendicular to the axis of the piston 16.
  • This particular arrangement makes it possible to ensure good abutment cooperation between the underside of the flange 42 and the abutment surface 44 formed on the inner wall of the end piece 24.
  • the flange 42 On its upper face, the flange 42 is shaped so as to be perfectly applied against the profile of the opening zone of the pinching member 14. Since in practice the elastic branches 28 of the pinching member have, near their free end, a bevelled internal face intended to facilitate the introduction of the upper end of the piston 16, the upper face of the collar 42 also has a central part affecting a slightly conical profile.
  • the upper free end of the piston 16 ends, beyond said flange 42, by a bulged portion 46 which is essentially cylindrical.
  • This bulged part 46 can also advantageously have a cone shape of very slight slope inverted with respect to the conical central profile 50.
  • This bulged part 46 only intended to facilitate the gripping of the piston 16 between the elastic branches 28 of the pinching member 14 has an axial dimension, extending from the free upper end of the piston 16 to the upper face of the flange 42, which remains significantly less than the depth of the central orifice 48 formed between the elastic branches 28 of the member pinching 14.
  • the lower face of the flange 42 comprises, in its central part, centering means, cooperating for example with the stop 44 formed on the inner wall of the end piece 24 with capillary tube 18.
  • centering means cooperating for example with the stop 44 formed on the inner wall of the end piece 24 with capillary tube 18.
  • the second spring is not yet fully compressed. Additional pressure exerted on the push button of the control rod 22 ensures complete compression of the second spring beyond the hard point corresponding to the position illustrated in FIG. 2.
  • the elastic branches 28 of the pinch member 14 always remains apart, which allows the release of the gripping of the piston 16, then during said complementary downward stroke these elastic branches 28 exert pressure on the flange 42, which is transmitted to the abutment surface 44 and therefore to the nozzle 24 which can thus be separated from the rest of the pipette.
  • Such an ejection can be obtained without any risk of loss of the nozzle in normal operation, for example by virtue of the particular embodiment described.
  • the inner surface of the upper part of the end piece 24 has at least one annular zone 54 projecting or recessed capable of cooperating elastically with a corresponding annular zone 56 recessed or projecting formed on the outer lateral surface of the lower part 12 of the pipette body.
  • These annular zones 54,56 projecting or recessed have been arranged so as to allow a large guide range of the tip 24 on the end of the pipette, for example of the order of 5 to 10 mm and preferably on the order of 6 to 8 mm.
  • these annular zones 54,56 projecting or recessed are also arranged so as to ensure a short engagement and disengagement stroke of the end piece 24, of the order of approximately 2 mm.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
EP82401913A 1981-10-28 1982-10-19 Präzisionspipette mit Verdrängungswirkung Expired EP0078724B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT82401913T ATE14994T1 (de) 1981-10-28 1982-10-19 Praezisionspipette mit verdraengungswirkung.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8120220A FR2515339A1 (fr) 1981-10-28 1981-10-28 Pipette de precision a deplacement positif
FR8120220 1981-10-28

Publications (2)

Publication Number Publication Date
EP0078724A1 true EP0078724A1 (de) 1983-05-11
EP0078724B1 EP0078724B1 (de) 1985-08-21

Family

ID=9263478

Family Applications (1)

Application Number Title Priority Date Filing Date
EP82401913A Expired EP0078724B1 (de) 1981-10-28 1982-10-19 Präzisionspipette mit Verdrängungswirkung

Country Status (7)

Country Link
US (1) US4474071A (de)
EP (1) EP0078724B1 (de)
JP (1) JPS5884049A (de)
AT (1) ATE14994T1 (de)
DE (1) DE3265643D1 (de)
FR (1) FR2515339A1 (de)
IE (1) IE53877B1 (de)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0226867A2 (de) * 1985-12-17 1987-07-01 Hamilton Bonaduz AG Pipette und Pipettiervorrichtung
EP0421785A2 (de) * 1989-10-04 1991-04-10 Drummond Scientific Company Pipettiervorrichtung
WO1991017833A2 (en) * 1990-05-21 1991-11-28 Pb Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
EP0494735A2 (de) * 1991-01-07 1992-07-15 Labsystems Oy Verdrängerpipette
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
WO2006030201A1 (en) 2004-09-13 2006-03-23 Microsample Ltd. Method and apparatus for sampling and analysis of fluids
WO2006087444A1 (fr) * 2005-02-18 2006-08-24 Gilson S.A.S. Embout pour pipette de prelevement, et pipette ainsi equipee
WO2010086380A1 (en) 2009-01-30 2010-08-05 Pronota N.V. Biomarker for diagnosis, prediction and/or prognosis of acute heart failure and uses thereof
WO2011048173A1 (en) 2009-10-21 2011-04-28 Pronota N.V. Mcam as a biomarker for fluid homeostasis
WO2011128357A2 (en) 2010-04-13 2011-10-20 Pronota N.V. Biomarkers for hypertensive disorders of pregnancy
FR3012883A1 (fr) * 2013-11-07 2015-05-08 Gilson Sas Systeme de pipetage a deplacement positif, presentant une conception facilitant la prehension du piston de l'ensemble capillaire-piston
EP2924439A1 (de) 2010-03-26 2015-09-30 Mycartis N.V. Ltbp2 als biomarker zur vorhersage oder prognose von mortalität
US9804064B2 (en) 2011-09-19 2017-10-31 Gilson Sas Positive displacement pipette having an improved ejection function
US9931625B2 (en) 2013-03-25 2018-04-03 Gilson Sas Ejection function for a positive-displacement pipetting system
EP3831486A1 (de) * 2019-12-06 2021-06-09 Eppendorf AG Pipettenspitzenfamilie umfassend pipettenspitzen für den gebrauch mit pipetten einer pipettenfamilie und pipettenfamilie umfassend pipetten für den gebrauch mit pipettenspitzen einer pipettenspitzenfamilie
EP3831487A1 (de) * 2019-12-06 2021-06-09 Eppendorf AG Pipette für den gebrauch mit einer pipettenspitze und pipettenspitze für den gebrauch mit einer pipette

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5192511A (en) * 1991-05-31 1993-03-09 Tri-Continent Scientific, Inc. Pipette tip and piston
US6186012B1 (en) 1997-12-30 2001-02-13 Battelle Memorial Institute Hand held sample tube manipulator, system and method
US6955077B2 (en) * 2002-05-09 2005-10-18 Quality Scientific Plastics, Inc. Pipette tip with an internal sleeve and method for forming same
US7641859B2 (en) * 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
US20090215192A1 (en) * 2004-05-27 2009-08-27 Stratos Biosystems, Llc Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution
DE102005041183B3 (de) * 2005-08-31 2007-01-04 Eppendorf Ag Pipettiervorrichtung
GB0605003D0 (en) * 2006-03-13 2006-04-19 Microsample Ltd Method and apparatus for piercing the skin and delivery or collection of liquids
DE102007042115B4 (de) * 2007-09-05 2010-12-02 Eppendorf Ag Pipette
US8899118B1 (en) * 2009-05-11 2014-12-02 Dan Seguin Multi-channel aspirating and dispensing instrument
DK3498376T3 (da) 2009-07-27 2021-12-20 Eppendorf Ag Sprøjte, sprøjtefamilie og doseringsindretning
US8524170B2 (en) * 2011-02-22 2013-09-03 Rainin Instrument, Llc Pipette and sealing tip
ES2692094T3 (es) 2012-12-09 2018-11-30 Chromedx Corp. Sistema automatizado de ultrafiltración
WO2015079477A1 (ja) * 2013-11-29 2015-06-04 ヤマハ発動機株式会社 吸引方法
JP6042562B2 (ja) * 2013-11-29 2016-12-14 ヤマハ発動機株式会社 吸引チップ
JP6678096B2 (ja) * 2016-12-13 2020-04-08 株式会社日立製作所 分注装置、分注方法、分注装置製造方法
JP7315625B2 (ja) 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット
GB2607893A (en) * 2021-06-11 2022-12-21 Spt Labtech Ltd Pipette tip for a liquid dispensing apparatus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0014120A1 (de) * 1979-01-19 1980-08-06 Marteau d'Autry, Eric Pipette mit positiver Verstellung
EP0032469A1 (de) * 1980-01-11 1981-07-22 Marteau d'Autry, Eric Pipette mit positiver Verschiebung

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2711124C2 (de) * 1977-03-15 1979-05-10 Labora Mannheim Gmbh Fuer Labortechnik, 6800 Mannheim Handpipette
FI56937C (fi) * 1978-08-04 1980-05-12 Suovaniemi Finnpipette Anordning foer att avlaegsna en engaongsspetsbehaollare fraon en pipett
FR2473124A1 (fr) * 1980-01-04 1981-07-10 Marteau D Autry Eric Dispositif de prelevement et de distribution de volumes reglables de liquides, a affichage numerique

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0014120A1 (de) * 1979-01-19 1980-08-06 Marteau d'Autry, Eric Pipette mit positiver Verstellung
EP0032469A1 (de) * 1980-01-11 1981-07-22 Marteau d'Autry, Eric Pipette mit positiver Verschiebung

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0226867A2 (de) * 1985-12-17 1987-07-01 Hamilton Bonaduz AG Pipette und Pipettiervorrichtung
EP0226867A3 (en) * 1985-12-17 1988-08-10 Hamilton Bonaduz Ag Pipette and pipetting system
EP0421785A2 (de) * 1989-10-04 1991-04-10 Drummond Scientific Company Pipettiervorrichtung
EP0421785A3 (en) * 1989-10-04 1991-06-26 Drummond Scientific Company Pipetter device
WO1991017833A2 (en) * 1990-05-21 1991-11-28 Pb Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
WO1991017833A3 (en) * 1990-05-21 1991-12-26 Pb Diagnostic Systems Inc Fluid dispensing system having a pipette assembly with preset tip locator
EP0494735A2 (de) * 1991-01-07 1992-07-15 Labsystems Oy Verdrängerpipette
EP0494735A3 (en) * 1991-01-07 1992-12-09 Labsystems Oy Positive-displacement pipette
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
WO2006030201A1 (en) 2004-09-13 2006-03-23 Microsample Ltd. Method and apparatus for sampling and analysis of fluids
US8092394B2 (en) 2004-09-13 2012-01-10 Microsample Ltd. Method and apparatus for sampling and analysis of fluids
WO2006087444A1 (fr) * 2005-02-18 2006-08-24 Gilson S.A.S. Embout pour pipette de prelevement, et pipette ainsi equipee
FR2882273A1 (fr) * 2005-02-18 2006-08-25 Gilson Sas Soc Par Actions Sim Embout pour pipette de prelevement, et pipette ainsi equipee
US8480972B2 (en) 2005-02-18 2013-07-09 Gilson S.A.S. Shaft for a sampling pipette
WO2010086380A1 (en) 2009-01-30 2010-08-05 Pronota N.V. Biomarker for diagnosis, prediction and/or prognosis of acute heart failure and uses thereof
WO2011048168A1 (en) 2009-10-21 2011-04-28 Pronota N.V. Biomarker for diagnosis, prediction and/or prognosis of acute heart failure and uses thereof
WO2011048173A1 (en) 2009-10-21 2011-04-28 Pronota N.V. Mcam as a biomarker for fluid homeostasis
EP2924439A1 (de) 2010-03-26 2015-09-30 Mycartis N.V. Ltbp2 als biomarker zur vorhersage oder prognose von mortalität
WO2011128357A2 (en) 2010-04-13 2011-10-20 Pronota N.V. Biomarkers for hypertensive disorders of pregnancy
US9804064B2 (en) 2011-09-19 2017-10-31 Gilson Sas Positive displacement pipette having an improved ejection function
US9931625B2 (en) 2013-03-25 2018-04-03 Gilson Sas Ejection function for a positive-displacement pipetting system
FR3012883A1 (fr) * 2013-11-07 2015-05-08 Gilson Sas Systeme de pipetage a deplacement positif, presentant une conception facilitant la prehension du piston de l'ensemble capillaire-piston
WO2015067579A1 (fr) * 2013-11-07 2015-05-14 Gilson Sas Système de pipetage à déplacement positif, présentant une conception facilitant la préhension du piston de l'ensemble capillaire-piston
CN105705241A (zh) * 2013-11-07 2016-06-22 吉尔松有限合伙公司 具有便于夹持毛细管-活塞组件的活塞的设计的容积式移液系统
US9931626B2 (en) 2013-11-07 2018-04-03 Gilson Sas Positive displacement pipetting system, having a design facilitating the gripping of the piston of the capillary-piston assembly
EP3831486A1 (de) * 2019-12-06 2021-06-09 Eppendorf AG Pipettenspitzenfamilie umfassend pipettenspitzen für den gebrauch mit pipetten einer pipettenfamilie und pipettenfamilie umfassend pipetten für den gebrauch mit pipettenspitzen einer pipettenspitzenfamilie
EP3831487A1 (de) * 2019-12-06 2021-06-09 Eppendorf AG Pipette für den gebrauch mit einer pipettenspitze und pipettenspitze für den gebrauch mit einer pipette
WO2021110902A1 (de) * 2019-12-06 2021-06-10 Eppendorf Ag Pipettenspitzenfamilie und pipettenfamilie

Also Published As

Publication number Publication date
US4474071A (en) 1984-10-02
IE822582L (en) 1983-04-28
IE53877B1 (en) 1989-03-29
ATE14994T1 (de) 1985-09-15
FR2515339A1 (fr) 1983-04-29
JPH0153102B2 (de) 1989-11-13
FR2515339B1 (de) 1984-01-20
DE3265643D1 (en) 1985-09-26
EP0078724B1 (de) 1985-08-21
JPS5884049A (ja) 1983-05-20

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