EP0078724B1 - Präzisionspipette mit Verdrängungswirkung - Google Patents

Präzisionspipette mit Verdrängungswirkung Download PDF

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Publication number
EP0078724B1
EP0078724B1 EP82401913A EP82401913A EP0078724B1 EP 0078724 B1 EP0078724 B1 EP 0078724B1 EP 82401913 A EP82401913 A EP 82401913A EP 82401913 A EP82401913 A EP 82401913A EP 0078724 B1 EP0078724 B1 EP 0078724B1
Authority
EP
European Patent Office
Prior art keywords
pipette
piston
flange
gripping device
capillary tube
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP82401913A
Other languages
English (en)
French (fr)
Other versions
EP0078724A1 (de
Inventor
Eric Marteau D'autry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT82401913T priority Critical patent/ATE14994T1/de
Publication of EP0078724A1 publication Critical patent/EP0078724A1/de
Application granted granted Critical
Publication of EP0078724B1 publication Critical patent/EP0078724B1/de
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the present invention relates to an improvement made to precision positive displacement pipettes intended for the sampling and distribution of small quantities of liquid.
  • such positive displacement precision pipettes are already known which are of the type comprising an essentially tubular pipette body at the lower end of which is arranged a calibrated capillary tube in which it moves in a sealed and against an upward elastic restoring force exerted by a main spring, a piston controlled in its downward movement by a control rod protruding from the upper end of said pipette body, as well as a clamping member of the upper end of the piston, which is of the type with elastic branches deviating radially by displacement against the force exerted by a second spring of resistance much greater than that of the main spring and which is integral in translation with said rod control.
  • Such a positive displacement pipette is for example described in EP-A-0 014120 belonging to the applicant.
  • the present invention specifically aims to achieve a positive displacement pipette which overcomes the aforementioned drawbacks.
  • the positive displacement precision pipette is characterized in that its piston has, in the vicinity of its upper end intended to be gripped by said gripping member, a collar capable of exerting abutment cooperation by its upper face with the free end of the elastic branches of the pinching member and by its lower face with an abutment surface formed on the inner surface of said capillary tube tip.
  • the positive displacement pipette comprises an essentially tubular body 10 which, in a particular embodiment, may consist of two separate parts which are secured to each other by screwing.
  • the upper part or housing (not shown) allows for example to incorporate a mechanism for adjusting and displaying the volume to be withdrawn and distributed, and the tubular lower part 12 is used mainly for guiding the nipping member 14 of the piston 16.
  • the particular adjustment and display mechanism is not part of the present invention and will therefore not be described here in detail. Such a mechanism is for example described in detail in EP-A-0 032 098 filed in the name of the applicant and the lessons of which are integrated into the present description.
  • a calibrated capillary tube 18 in which moves, in a sealed manner and against an upward elastic restoring force exerted by a main spring 20, a piston 16 controlled in its movement by a control rod 22 projecting from the upper end of the case of the pipette body 10.
  • the pipette has a tip 24 which ends in a calibrated capillary tube 18 and which is able to cooperate by fitting with the free lower end of the pipette body 10.
  • a tip 24 will be made of a thermoplastic material chosen to resist the samples with which it comes into contact. It will for example be made of polypropylene or any other semi-rigid thermoplastic material.
  • the piston 16 has at least one sealing zone 26 coming into intimate contact with the inner surface of the capillary tube 18.
  • a piston 16 can for example advantageously be produced in the form of a 'a stainless steel wire having a coating, for example a coating of polyethylene or teflon, at the sealing zone 26.
  • a coating can for example affect a shape generally cylindrical or else may be equipped at its periphery with at least one annular sealing lip coming into intimate contact with the internal surface of the capillary tube 18.
  • Such a type of pipette also comprises a clamping member 14 of the upper end of the piston 16, which is of the type with elastic branches 28 deviating radially by displacement against the force exerted by a second spring (not shown) resistance much higher than that of the main spring 20. It is clear that such a clamping member 14 must be secured in translation with the control rod 22 of the pipette.
  • this pinching member 14 can for example be in the form of a self-contained cartridge comprising a clamp proper ending at its lower end by elastic branches 28 and of which the other end is screwed into a clamp holder.
  • This clamp support is able to slide, against the force exerted by the second spring and according to a partial stroke in the upper part of an orifice formed in the clamp holder 30.
  • the latter clamp holder 30 slides itself inside the lower part 12 of the pipette body 10 against the force exerted by the main spring 20. It is important that the end of the elastic branches 28 has an outwardly flaring surface suitable for to be applied tightly against the conical lower opening 32 of the clamp holder 30, and this precisely under the action of the force exerted by the second spring.
  • the assembly of this cartridge which constitutes the pinching member of the piston 16 is elastically urged upwards under the action of the force exerted by the main spring 20.
  • the holder clamp 30 has a shoulder 34 against which the main spring rests. By its lower end, the main spring 20 comes into fixed support on a shoulder 36 formed on the internal surface of the lower part 12 of the pipette body 10 , in the vicinity of its free lower end.
  • the clamp holder 30 can advantageously come into upper abutment against an adjustment screw (not shown), the variable vertical position of which makes it possible to adjust the useful stroke for taking and dispensing the liquid sample.
  • the present invention is however not limited to such a type of adjustable pipette, but can of course also extend to fixed pipettes, i. e. pipettes whose adjusting screw would be replaced by an upstroke limit stop which remains fixed in position relative to the pipette body 10.
  • the stop for limiting down the useful stroke for dispensing the pipette is made at a shoulder 38 formed in the vicinity of the lower part of the clamp holder 30.
  • This shoulder 38 is able to cooperate with the shoulder corresponding 36 formed in the vicinity of the lower end of the pipette body 10.
  • Such a lower limit position of the clamp-holder member 14 is illustrated in FIG. 2. It will be noted that this position is obtained at the end of the compression complete with main spring 20.
  • the opening 40 of the free lower end of the pipette body 10 is arranged so as to allow at least a portion of the clamping member 14 to be passed which, after compression of the second spring and at the end of the downward stroke, ensures the simultaneous ejection of said end piece 24 and of the piston 16 which remains trapped in the capillary tube 18.
  • the piston 16 has a flange 42 located near its upper end intended to be gripped by the elastic branches 28 of the clamping member 14.
  • This flange 42 has an upper face capable of cooperating with the free end of the elastic branches 28 of the clamping member 14, as well as a lower face capable of cooperating with a abutment surface 44 formed on the inner wall of the end piece 24 with capillary tube 18, preferably in the vicinity of the junction between the upper flared part of the end piece and the capillary tube 18 proper.
  • the flange 42 has a transverse dimension substantially equal to the outer diameter of the elastic branches 28, in the separated state, of the clamping member 14.
  • This flange 42 has on its underside at least one peripheral annular zone defining an abutment surface which is substantially perpendicular to the axis of the piston 16. This particular arrangement makes it possible to ensure good abutment cooperation between the underside of the flange 42 and the abutment surface 44 formed on the inner wall of the end piece 24.
  • the flange 42 is shaped so as to be perfectly applied against the profile of the opening zone of the pinching member 14.
  • the elastic branches 28 of the pinching member have, in the vicinity of their free end, a bevelled internal face intended to facilitate the introduction of the upper end of the piston 16, the upper face of the flange 42 also has a central part affecting a slightly conical profile.
  • the upper free end of the piston 16 ends, beyond said flange 42, by a bulged portion 46 which is essentially cylindrical.
  • This bulged part 46 can also advantageously have a cone shape of very slight slope inverted with respect to the conical central profile 50.
  • This bulged part 46 only intended to facilitate the gripping of the piston 16 between the elastic branches 28 of the pinching member 14 has an axial dimension, extending from the free upper end of the piston 16 to the upper face of the flange 42, which remains significantly less than the depth of the central orifice 48 formed between the elastic branches 28 of the member pinching 14.
  • the lower face of the flange 42 comprises, in its central part, centering means, cooperating for example with the stop 44 formed on the inner wall of the end piece 24 with capillary tube 18.
  • centering means cooperating for example with the stop 44 formed on the inner wall of the end piece 24 with capillary tube 18.
  • annular zone 54 projecting or recessed capable of cooperating elastically with a corresponding annular zone 56 recessed or projecting formed on the surface lateral lateral view of the lower part 12 of the pipette body.
  • These annular zones 54, 56 projecting or recessed have been arranged so as to allow a large guide range of the tip 24 on the end of the pipette, for example of the order of 5 to 10 mm and preferably on the order of 6 to 8 mm.
  • these annular zones 54, 56 projecting or recessed are also arranged so as to ensure a short engagement and disengagement stroke of the end piece 24, of the order of approximately 2 mm.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (9)

1. Präzisionspipette mit Verdrängungswirkung, die zur Entnahme und zur Verteilung kleiner Flüssigkeitsmengen bestimmt ist, mit einem im wesentlichen tubusartigen Pipettenkörper an ihrem unteren Ende, an welchem eine Muffe mit geeichtem Kapillarrohr abnehmbar angeschäftet ist, in dem sich ein Kolben wasserdicht und gegen eine nach oben gerichtete elastische Rückstellkraft, die durch eine Hauptfeder ausgeübt wird, bewegt, wobei die Bewegung von einem Steuerhebel gesteuert wird, welcher dabei den obersten Rand des genannten Pipettenkörpers überschreitet, sowie einem Klemmorgan am oberen Ende des Kolbens, welches vom Typ elastischer Schenkel ist, die sich durch Verschiebung gegen eine Kraft, die von einer zweiten Widerstandsfeder ausgeübt wird, die wesentlich stärker als die Kraft der Hauptfeder ist, radial spreizen, und welches bei der Bewegung mit dem genannten Steuerhebel fest verbunden ist, wobei die Öffnung des unteren freien Endes des Pipettenkörpers die Passage wenigstens eines Teiles des Klemmorgans erlaubt, das nach Kompression der zweiten Feder und in der Nähe des Endes seines Weges nach unten, die gleichzeitige Ausstoßung der genannten Muffe und des Kolbens, der im Kapillarrohr gefangen bleibt, sicherstellen will, dadurch gekennzeichnet, daß der Kolben (16) in der Nähe seines oberen Endes, das dazu bestimmt ist, von besagtem Klemmorgan (14) gegriffen zu werden, einen Kragen (42) aufweist, dessen Oberseite mit dem freien Ende der elastischen Schenkel (28) des Klemmorgans (14), und dessen Unterseite mit einer Anschlagfläche (44), die auf der Innenkante der genannten Muffe (24) im Kapillarrohr (18) angebracht ist, zum Anschlag gebracht werden kann.
2. Pipette nach Anspruch 1, dadurch gekennzeichnet, daß der genannte Kragen (42) eine transversale Ausdehnung aufweist, die im wesentlichen gleich dem äußeren Durchmesser der elastischen Schenkel (28) des Klemmorgans (14), im gespreizten Zustand, ist.
3. Pipette nach einem der Ansprüche 1 und 2, dadurch gekennzeichnet, daß der genannte Kragen (42) eine Unterseite aufweist, von der wenigstens eine ringförmige periphere Zone eine Anschlagfläche definiert, die im wesentlichen senkrecht zu der Achse des Kolbens (16) ist.
4. Pipette nach einem der Ansprüche 1 bis 3, dadurch gekennzeichnet, daß der genannte Kragen (42) eine Oberseite aufweist, die so ausgebildet ist, daß sie sich vollkommen gegen das Profil der Öffnungszone des Klemmorgans (14) anlegt.
5. Pipette nach Anspruch 4, dadurch gekennzeichnet, daß der genannte Kragen (42) eine Oberseite aufweist, deren zentraler Teil ein konisches Profil aufweist.
6. Pipette nach einem der Ansprüche 1 bis 5, dadurch gekennzeichnet, daß das freie obere Ende des Kolbens (16) jenseits des Kragens (42) in einem im wesentlichen zylindrisch gewölbten Teil (46) endet, der dazu bestimmt ist, in die Öffnung des Klemmorgans (14) einzudringen, und daß die axiale Ausdehnung dieses gewölbten Teils (46), das sich bis zur Oberseite des Kragens (42) erstreckt, kleiner ist als die Tiefe der Öffnung (48) des Klemmorgans (14).
7. Pipette nach einem der Ansprüche 1 bis 6, dadurch gekennzeichnet, daß die Unterseite des genannten Kragens (42), in ihrem zentralen Teil, Mittel zur Zentrierung des oberen Teils des Kolbens (16) enthält.
8. Pipette nach Anspruch 7, dadurch gekennzeichnet, daß die genannten Mittel zur Zentrierung die Form eines zentralen konischen Profils (50) aufweisen, das auf der Unterseite des Kragens (42) angebracht ist, wobei der größte Durchmesser dieses zentralen konischen Profils (50) etwas kleiner als der innere Durchmesser des Kapillarrohres (18) ist.
9. Pipette nach einem der Ansprüche 1 bis 8, dadurch gekennzeichnet, daß der Kragen (42), der auf seiner Oberseite angeordnete gewölbte Teil (46) und die an seiner Unterseite angeordneten Mittel zur Zentrierung (50) in ein und demselben aus Kunststoff gegossenen Stück verwirklicht sind.
EP82401913A 1981-10-28 1982-10-19 Präzisionspipette mit Verdrängungswirkung Expired EP0078724B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT82401913T ATE14994T1 (de) 1981-10-28 1982-10-19 Praezisionspipette mit verdraengungswirkung.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8120220 1981-10-28
FR8120220A FR2515339A1 (fr) 1981-10-28 1981-10-28 Pipette de precision a deplacement positif

Publications (2)

Publication Number Publication Date
EP0078724A1 EP0078724A1 (de) 1983-05-11
EP0078724B1 true EP0078724B1 (de) 1985-08-21

Family

ID=9263478

Family Applications (1)

Application Number Title Priority Date Filing Date
EP82401913A Expired EP0078724B1 (de) 1981-10-28 1982-10-19 Präzisionspipette mit Verdrängungswirkung

Country Status (7)

Country Link
US (1) US4474071A (de)
EP (1) EP0078724B1 (de)
JP (1) JPS5884049A (de)
AT (1) ATE14994T1 (de)
DE (1) DE3265643D1 (de)
FR (1) FR2515339A1 (de)
IE (1) IE53877B1 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH671526A5 (de) * 1985-12-17 1989-09-15 Hamilton Bonaduz Ag
US5104625A (en) * 1989-10-04 1992-04-14 Drummond Scientific Company Pipetter device
US5200151A (en) * 1990-05-21 1993-04-06 P B Diagnostic Systems, Inc. Fluid dispensing system having a pipette assembly with preset tip locator
FI86812C (fi) * 1991-01-07 1992-10-26 Labsystems Oy Foertraengningspipett
US5192511A (en) * 1991-05-31 1993-03-09 Tri-Continent Scientific, Inc. Pipette tip and piston
US5983733A (en) * 1996-11-15 1999-11-16 Hamilton Company Manual pipette
US6186012B1 (en) 1997-12-30 2001-02-13 Battelle Memorial Institute Hand held sample tube manipulator, system and method
US6955077B2 (en) * 2002-05-09 2005-10-18 Quality Scientific Plastics, Inc. Pipette tip with an internal sleeve and method for forming same
US7641859B2 (en) * 2004-02-11 2010-01-05 Matrix Technologies Corporation Pipette tip mounting and ejection assembly and associated pipette tip
US20090215192A1 (en) * 2004-05-27 2009-08-27 Stratos Biosystems, Llc Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution
GB0420256D0 (en) 2004-09-13 2004-10-13 Cassells John M Method and apparatus for sampling and analysis of fluids
FR2882273B1 (fr) * 2005-02-18 2007-06-01 Gilson Sas Soc Par Actions Sim Embout pour pipette de prelevement, et pipette ainsi equipee
DE102005041183B3 (de) * 2005-08-31 2007-01-04 Eppendorf Ag Pipettiervorrichtung
GB0605003D0 (en) * 2006-03-13 2006-04-19 Microsample Ltd Method and apparatus for piercing the skin and delivery or collection of liquids
DE102007042115B4 (de) * 2007-09-05 2010-12-02 Eppendorf Ag Pipette
DK2391892T3 (en) 2009-01-30 2017-04-24 Mycartis N V BIOMARKET FOR DIAGNOSIS, PREDICTION AND / OR PROJECTS OF ACUTE HEART FAILURE AND USE thereof
US8899118B1 (en) * 2009-05-11 2014-12-02 Dan Seguin Multi-channel aspirating and dispensing instrument
EP3498376B1 (de) 2009-07-27 2021-09-15 Eppendorf AG Spritze, spritzenfamilie und dosiervorrichtung
DK2491401T3 (en) 2009-10-21 2018-06-25 Mycartis N V MCAM as a biomarker for fluid homeostasis
WO2011117371A2 (en) 2010-03-26 2011-09-29 Pronota N.V. Ltbp2 as a biomarker for renal dysfunction
CA2793487A1 (en) 2010-04-13 2011-10-20 Pronota N.V. Biomarkers for hypertensive disorders of pregnancy
US8524170B2 (en) * 2011-02-22 2013-09-03 Rainin Instrument, Llc Pipette and sealing tip
FR2980123B1 (fr) 2011-09-19 2013-10-11 Gilson Sas Pipette a deplacement positif presentant une fonction d'ejection amelioree
EP2928587B8 (de) 2012-12-09 2019-01-23 Relay Medical Corp. Automatisiertes ultrafiltrationssystem
FR3003643B1 (fr) * 2013-03-25 2015-04-17 Gilson Sas Systeme de pipetage a deplacement positif presentant une fonction d'ejection amelioree
FR3012883B1 (fr) * 2013-11-07 2015-12-25 Gilson Sas Systeme de pipetage a deplacement positif, presentant une conception facilitant la prehension du piston de l'ensemble capillaire-piston
WO2015079477A1 (ja) * 2013-11-29 2015-06-04 ヤマハ発動機株式会社 吸引方法
EP3075839B1 (de) * 2013-11-29 2019-03-20 Yamaha Hatsudoki Kabushiki Kaisha Absaugspitze
JP6678096B2 (ja) * 2016-12-13 2020-04-08 株式会社日立製作所 分注装置、分注方法、分注装置製造方法
EP3831486A1 (de) * 2019-12-06 2021-06-09 Eppendorf AG Pipettenspitzenfamilie umfassend pipettenspitzen für den gebrauch mit pipetten einer pipettenfamilie und pipettenfamilie umfassend pipetten für den gebrauch mit pipettenspitzen einer pipettenspitzenfamilie
EP3831487B1 (de) * 2019-12-06 2024-02-07 Eppendorf SE Pipette für den gebrauch mit einer pipettenspitze und pipettenspitze für den gebrauch mit einer pipette
JP7315625B2 (ja) 2020-07-14 2023-07-26 エッペンドルフ エスイー 一体型プランジャを備えたピペット・チップとともに使用するためのピペット
GB2607893A (en) * 2021-06-11 2022-12-21 Spt Labtech Ltd Pipette tip for a liquid dispensing apparatus

Family Cites Families (5)

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Publication number Priority date Publication date Assignee Title
DE2711124C2 (de) * 1977-03-15 1979-05-10 Labora Mannheim Gmbh Fuer Labortechnik, 6800 Mannheim Handpipette
FI56937C (fi) * 1978-08-04 1980-05-12 Suovaniemi Finnpipette Anordning foer att avlaegsna en engaongsspetsbehaollare fraon en pipett
FR2446672A1 (fr) * 1979-01-19 1980-08-14 Marteau D Autry Eric Pipette a deplacement positif
FR2473124A1 (fr) * 1980-01-04 1981-07-10 Marteau D Autry Eric Dispositif de prelevement et de distribution de volumes reglables de liquides, a affichage numerique
FR2474340A1 (fr) * 1980-01-11 1981-07-31 Marteau D Autry Eric Pipette a deplacement positif perfectionnee

Also Published As

Publication number Publication date
JPH0153102B2 (de) 1989-11-13
FR2515339A1 (fr) 1983-04-29
FR2515339B1 (de) 1984-01-20
IE53877B1 (en) 1989-03-29
IE822582L (en) 1983-04-28
US4474071A (en) 1984-10-02
DE3265643D1 (en) 1985-09-26
JPS5884049A (ja) 1983-05-20
EP0078724A1 (de) 1983-05-11
ATE14994T1 (de) 1985-09-15

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