EP0071538B1 - Appareil de transfert d'éléments de reproduction animale, tels que des embryons - Google Patents

Appareil de transfert d'éléments de reproduction animale, tels que des embryons Download PDF

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Publication number
EP0071538B1
EP0071538B1 EP82401420A EP82401420A EP0071538B1 EP 0071538 B1 EP0071538 B1 EP 0071538B1 EP 82401420 A EP82401420 A EP 82401420A EP 82401420 A EP82401420 A EP 82401420A EP 0071538 B1 EP0071538 B1 EP 0071538B1
Authority
EP
European Patent Office
Prior art keywords
tube
reservoir tube
main body
rigid
sheath
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
EP82401420A
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German (de)
English (en)
French (fr)
Other versions
EP0071538A1 (fr
Inventor
Bertrand Cassou
Maurice Cassou
Robert Cassou
Claude Lemarie
Régis Leprince
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Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT82401420T priority Critical patent/ATE11637T1/de
Publication of EP0071538A1 publication Critical patent/EP0071538A1/fr
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Publication of EP0071538B1 publication Critical patent/EP0071538B1/fr
Expired legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/04Instruments or methods for reproduction or fertilisation for embryo transplantation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/027Devices for injecting semen into animals, e.g. syringes, guns, probes

Definitions

  • the present invention relates to devices for transferring elements of animal reproduction, such as embryos (from a donor reproductive female and to be placed in a carrier female) or animal semen (taken from a male for the purpose of 'artificial insemination). It relates more particularly to devices of the purely mechanical ejection type, comprising a hollow and rigid main body, of generally cylindrical shape, at one end of which is fixed a flexible tube which extends the main body outwards, this tube having to serve as reservoir to the reproduction elements, while this main body internally receives, at its other end, a piston rod with which is associated a sliding piston, the free end of the reservoir tube comprising, moreover, an ejection nozzle with axial passage, the body being able, moreover, to be advantageously surrounded externally, over at least a large part of its length, by a protective sheath fixed on it, which is changed at each operation, which avoids sterilization of the apparatus.
  • the tube -reservoir is housed, over most of its length, inside the rigid body, and the piston is directly actuated by the end of the rod whose length is roughly equal to that of the body (similarly besides that of the protective sheath, when provided, in which case the end piece is, moreover, interposed between the end of the reservoir tube and that of the sheath which both protrude slightly from the body) .
  • the entire apparatus is rigid over its entire length and the rod and piston assembly is used at least to push the reproductive elements into the womb of the breeding female, in the case of semen , in that of the carrier female, in the case of an embryo.
  • This same rod and piston assembly may, moreover, have already been used, in addition, for the aspiration of the semen in a particular embodiment of the first case.
  • it is a reservoir tube previously filled with semen, or a suspension containing the embryo, and in general stored under refrigeration, after removal from the male or the breeding female, which is fixed on the body of the device, a tampon which seals the end of the tube then serving as a piston during ejection.
  • the free end of this device should, in principle, be inserted beyond the vagina and cervix, as far as possible inside the uterus of the animal, to ensure a deep deposit of the semen or embryo and therefore facilitate reproduction or implantation.
  • the uterine lining is extremely fragile (particularly in the case of an embryo transfer, which should normally take place outside the period of oestrus), so that these known devices risk, made of their rigid end, to cause a lesion of this mucosa with all the consequences that this entails.
  • the known devices for example the aforementioned patent FR -A? 2477006
  • the known devices in fact only allow the establishment of the embryo in the carrier female, the collection on the female reproductive using an entirely separate device and operating of a completely different type, since by fluid circulation (such as that described in the French patent of holders FP-AN No. 2477005).
  • the delivery device must receive a reservoir tube already filled with the embryo suspension.
  • the main object of the present invention is to minimize the risk of damage to the uterine lining and, for this purpose, it relates to a transfer device of the aforementioned type, in which a rigid main body is surrounded by a second rigid body. characterized in that this main body can slide inside said second body which has approximately the same length as the latter, while the piston rod comprises a front section which is flexible and has a length at least equal to that of the tank tube.
  • the device can, in in addition, include a fixing sleeve rigid which can surround the reservoir tube and which has, at one end, means for retaining the nozzle fixed to the front end of the reservoir tube, and at the other end, means for pressing on the front end of the outer body, this sheath favoring the maintenance of the reservoir tube, which is flexible, while it is fixed to the end of the main body, for example by interlocking, while simultaneously ensuring perfect asepsis of the operation .
  • a fixing sleeve rigid which can surround the reservoir tube and which has, at one end, means for retaining the nozzle fixed to the front end of the reservoir tube, and at the other end, means for pressing on the front end of the outer body, this sheath favoring the maintenance of the reservoir tube, which is flexible, while it is fixed to the end of the main body, for example by interlocking, while simultaneously ensuring perfect asepsis of the operation .
  • a protective sheath it can very advantageously be arranged around the external body, so as to protrude slightly at the front thereof, by presenting at its front end a rounded inner hem, while it is, moreover, fixed on the same outer body at its opposite end, which allows, when the main body recedes, to fully return the reservoir tube inside this sheath , the hem of the latter avoiding any lesion of the mucosa during introduction into the uterus.
  • the end piece fixed to the end of the reservoir tube has, at the front, a rigid spherical head, which can pass elastically through the hem of the sheath, while its central passage opens out.
  • the spherical head due to its shape, progresses easily and without damage in the uterine lumen.
  • the object of the invention is also to make it possible to carry out, using the same apparatus, the aspiration sampling in the empty reservoir tube of the suspension containing the embryo (s), and this in an intermediate storage container, without that it is necessary to use other specialized devices for this purpose.
  • the apparatus according to the invention is, furthermore, characterized in that the flexible front section of the piston rod carries, at its front end, the ejection piston and is separated from the rear section of the rod which is rigid.
  • the subassembly of the device constituted by the reservoir tube and the flexible front section of the rod can be separated from the rest of the device to operate like a syringe for aspirating, in a storage container, the suspension containing the embryo, this sub-assembly then being reattached to the rest of the device to allow introduction and ejection in the manner already indicated.
  • the apparatus may also comprise a rigid guide sleeve which can surround the reservoir tube and which has, at one end, means for retaining the end of the reservoir tube opposite to that which must receive the tip, this sheath favoring the maintenance of the reservoir tube during the aspiration of the suspension, which avoids, during handling, any twisting which could block the aspiration of the embryo, while ensuring the asepsis of this operation.
  • a rigid guide sleeve which can surround the reservoir tube and which has, at one end, means for retaining the end of the reservoir tube opposite to that which must receive the tip, this sheath favoring the maintenance of the reservoir tube during the aspiration of the suspension, which avoids, during handling, any twisting which could block the aspiration of the embryo, while ensuring the asepsis of this operation.
  • the apparatus represented by the figures essentially consists of an external body 1 (fig. 1), a main body 2 (fig. 2), a rigid piston rod element 3 (fig. 3), a reservoir tube 4 (fig. 4), a flexible front section of piston rod 5 (fig. 5), a nozzle 6 for the reservoir tube (fig. 6), and an ejection piston 7 (fig. 7), these various elements, shown separately in Figs. 1 to 7, being, moreover, supplemented by a suction and fixing tube 8 (fig. 8 to 12), a protective sheath 9 (fig. 11 to 13), and finally a clamping screw 10 (fig . 11 to 13).
  • the outer body 1 is a stainless steel body, hollow and rigid, of generally cylindrical shape, and more particularly constituted by a tube 1 a extending over its entire length and on the rear end 1 b from which is force fitted.
  • a sheath 1c also made of stainless steel, which has a frustoconical interlocking surface 1 d at the front, converging towards the front and, at the rear, an operating head 1 e, of annular shape, which has a radial tapping 1f also passing through the tube 1a, so as to open into the lumen of the latter, the central portion of this sheath 1c finally connecting it radially and outwards to the frustoconical bearing 1 d by a shoulder 1 g.
  • the main body 2 (fig. 2) is also formed by a stainless steel body, hollow and rigid, and also of generally cylindrical shape, but more particularly constituted by a tube 2a whose outside diameter corresponds to the inside diameter of the tube 1a, and at the rear end 2b of which is fixed an operating head 2c, of annular shape, and also made of stainless steel, while at its front end, this same tube has three distinct annular grooves separated by two interlocking surfaces frustoconical 2d and 2e, which converge forward and which connect to the grooves behind them by shoulders 2f.
  • the tube 2a is relatively longer than the tube 1a of the outer body, so that when the main body 2 is introduced into this outer body 1, as shown in FIG.
  • the rear piston rod element 3 (FIG. 3) is also constituted by a hollow and rigid tube 3a, made of stainless steel, on the rear end 3b of which is fixed an operating head of annular shape 3c, also in stainless steel, while at its front end 3d, this tube has a wider bore 3e over a certain length.
  • the outer diameter of this tube 3a corresponds to the inner diameter of the tube 2a of the main body, and its length is somewhat shorter than that of the outer body 1, so that when this rear piston rod element 3 is introduced into the inside the main body 2 until, as also shown in FIG. 11, the two gripping heads 2c and 3c, forming abutment means, come to bear one on the other, the front end of the tube 3 remains slightly set back relative to that of the body 1.
  • the reservoir tube 4 (fig. 4) is a perfectly cylindrical tube over its entire length, and made of a flexible plastic material, such as flexible polyvinyl chloride. Its outer diameter is substantially equal to that of the main body 2 and its inner diameter substantially equal to the diameter of the grooves 2g, located at the front end 2h of this tube, so that, as shown in fig. 11, the rear end 4a of the reservoir tube can be fitted elastically over the two frustoconical bearing surfaces 2d and 2e of the main body, until it comes into abutment against the rear shoulder of the rear groove 2g.
  • the length of this tube is provided so as to correspond, on the one hand, to a normal volume of the suspension containing the embryo (s) that this tube must contain, and, on the other hand, to the average length of the genital tract of the female.
  • the flexible front section of piston rod 5 (fig. 5) consists of a solid rod or a rod, also made of flexible plastic, such as flexible polyvinyl chloride.
  • the diameter of this rod is quite clearly less than the inside diameter of the reservoir tube 4, so that, when this rod is disposed inside this tube, as shown in FIG. 11, there remains a substantial radial clearance between them, this same diameter of the rod corresponding, moreover, very precisely to the diameter of the bore 3e of the rear piston rod element 3, which allows it to be able to come fully engage it, as shown in fig. 11.
  • the length of this rod is approximately of the same order of magnitude as that of the reservoir tube 4.
  • the end piece 6 (fig. 6) is a piece of stainless steel, of generally short cylindrical shape and which comprises, at its rear end, a frustoconical interlocking surface 6a, converging towards the rear and separated from the current part cylindrical by a groove 6b, while at its front end is welded a spherical head also made of stainless steel 6c, of slightly larger diameter, a central passage 6d finally extending over the greater part of this endpiece to open axially at the rear, while it opens on the side and at the rear of the spherical head 6c, using a 6th elbow.
  • the diameter of the current part of the end piece corresponds to that of the reservoir tube 4, so that, as still shown in FIG. 11, the front end 4b of this tube can come to fit elastically, over the frustoconical surface 6a, and until it comes into abutment against the front shoulder of the groove 6b.
  • the piston rod 7 (FIG. 7) is a plastic part, of relatively short cylindrical general shape, comprising at the front a frustoconical head 7a, while at the rear there is arranged axially a blind bore 7b, diameter corresponding to that of the rod 5, so that the front end 5a of the latter can come to fit there, as shown in FIG. 8.
  • the protective sheath 9 is a thin cylindrical sheath, made of a semi-rigid plastic material, such as semi-rigid polyvinyl chloride. Its diameter corresponds substantially to that of the current part 1 c of the outer body 1, so that, as shown in FIG. 11, this sheath can come to be fixed elastically, by its rear end 9a, over the frustoconical surface 1d of this body, and until it comes into abutment against the head 1e.
  • the length of this sheath is such that, in this latter position, its front end 9b comes substantially at the level of the groove 2g rear of the main body 2, and consequently of the rear edge of the reservoir tube 4 when the latter has come 'fitting over this body 2.
  • This same front end 9b of the sheath has a rounded hem 9c, rounded folded inwards, which comes into contact with said rear edge of the tube 4.
  • the suction and fixing sleeve 8 (fig. 8) consists of a relatively rigid plastic tube, the length of which is roughly equal to that of the reservoir tube 4, and the current diameter of which is relatively greater than that of this same tube. At one end 8a, this sheath has a cylindrical constriction, with a diameter still greater than that of the tube 4 but corresponding, on the other hand, to that of the hem 9c of the sheath, so that these two parts can bear the one against the other, as shown in fig. 11.
  • this sheath has another constriction, of frustoconical shape, the end of which provided with a hem has a diameter corresponding substantially to the diameter of the reservoir tube 4, so as to be able to act as a support either to the latter itself (fig. 4), or to the spherical head 6c of its tip (fig. 10 and 11).
  • the screw 10 As for the screw 10 (fig. 11), it has a threaded portion 10a, which can be screwed into the internal thread 1f of the outer body 1, and an operating head 10b.
  • the operator positions, using sterilized forceps, the end piece 6 terminated by its spherical head 6c, at the front end 4b of the tube 4, the fixing being done by elastic fitting of the tube 4 on the frustoconical surface 6a (fig. 9).
  • the operator introduces the sub-assembly into the same rigid sheath 8, but this time by its opposite end, so that the end piece 6 comes to rest, by its spherical head 6a, against the hem of the frustoconical constriction 8b of this sheath (fig. 10).
  • the operator then brings the flexible sub-assembly, held by the rigid sheath, facing the rest of the apparatus consisting of the sheath 9, the external body 1, the main body 2 and the piston rod 3, already introduced respectively. one inside the other, and in support, by their operating heads, as shown in fig. 11, the screw 10, which is fixed in the head 1 of the external body, also passing through a hole 2i in the main body to come to bear at its end on the piston rod 3, which ensures the assembly of the assembly.
  • the sheath 8 which came to bear on the hem 9c of the sheath 9
  • the operator guides the rear end 4a of the reservoir tube on the frustoconical surfaces 2d and 2e of the main body, up to ensure elastic fitting of this tube on this body (fig. 11).
  • the operator releases the screw 10 and very gently pushes, using its head 2c, the main body 2 which carries, on its front end, the reservoir tube 4, in a forward movement, and this as much as necessary so that the nozzle 6 and its lateral ejection orifice 6e reach the optimal ejection position.
  • the flexibility of the flexible sub-assembly 4.5 makes it possible to follow the meanders and the curvature of the uterine horn.
  • the body 1 and the sheath 9 of the embodiment described above are replaced by a single piece constituted by a modification of the external body (fig. 14).
  • This metal sleeve extends over a length which is of the order of magnitude of its outside diameter and successively presents, in the direction of the free end, a divergent frustoconical surface with a very slight slope 9'a, then a clearly rounded fillet shorter 9'b which is flush with the edge of the tube 1 '.
  • This sleeve has an outer surface which is carefully polished, while it is also glued, also carefully, on the end of the tube 1 '.
  • This sleeve, projecting from the tube, aims to advantageously replace the rounded hem 9c of the sheath 9 used in the previous embodiment, as will be described later.
  • the nozzle 6 of FIG. 6 is, in the present variant, replaced by a tip 6 'which differs from it only in that the spherical head 6c is here replaced by an end 6'a of the tip itself, integrally formed with it and having a pear or shell shape, that is to say successively in the direction of its free end, a bead 6'b of diameter slightly greater than that of the cylindrical body of the end piece, a converging frustoconical part 6 'c and a 6' spherical cap end.
  • the endpiece 6 ' has the same structure as the endpiece of FIG. 6 (socket 6a, groove 6b and central passage 6d), as well as the tube 1 'of fig. 14 which also presents the other elements of the tube of the external body of FIG. 1 (socket 1 d and head 1 e).
  • the shell-shaped head 6 ′ a of the nozzle 6 ′ comes to engage inside the end of the body outside 1 '(as in the previous embodiment, the spherical head 6a came into contact inside the hem 9c of the sheath), so as to ensure a perfect seal preventing the rise of vaginal mucus and cervical mucus . perfect preventing the rise of vaginal mucus and cervical mucus.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Reproductive Health (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Transplantation (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Prostheses (AREA)
  • Feeding And Watering For Cattle Raising And Animal Husbandry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Infusion, Injection, And Reservoir Apparatuses (AREA)
EP82401420A 1981-07-31 1982-07-29 Appareil de transfert d'éléments de reproduction animale, tels que des embryons Expired EP0071538B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT82401420T ATE11637T1 (de) 1981-07-31 1982-07-29 Geraet fuer die verpflanzung von elementen fuer die tierfortpflanzung, wie embrios.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8114930A FR2510393A1 (fr) 1981-07-31 1981-07-31 Appareil de transfert d'elements de reproduction animale, tels que des embryons
FR8114930 1981-07-31

Publications (2)

Publication Number Publication Date
EP0071538A1 EP0071538A1 (fr) 1983-02-09
EP0071538B1 true EP0071538B1 (fr) 1985-02-06

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EP82401420A Expired EP0071538B1 (fr) 1981-07-31 1982-07-29 Appareil de transfert d'éléments de reproduction animale, tels que des embryons

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EP (1) EP0071538B1 (https=)
AT (1) ATE11637T1 (https=)
DE (1) DE3262220D1 (https=)
FR (1) FR2510393A1 (https=)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2556596B1 (fr) * 1983-12-16 1986-12-05 Cassou Robert Instruments d'action gynecologique, notamment de transfert d'embryons
FR2574656B1 (fr) * 1984-12-13 1988-08-05 Cassou Robert Sonde gynecologique notamment pour l'injection de semence ou d'embryons dans la cavite des animaux, tels que les juments
FR2575063B1 (fr) * 1984-12-21 1988-07-01 Cassou Robert Sonde gynecologique pour insemination artificielle, notamment pour porcins
FR2578516B1 (fr) * 1985-03-06 1987-12-04 Cassou Robert Dispositif de conditionnement et de transfert de produit liquide, applicable notamment en medecine veterinaire
FR2609885B1 (fr) * 1987-01-22 1989-04-14 Cassou Robert Instrument pour l'insemination artificielle, le transfert d'embryons ou le prelevement de liquides folliculaires chez les mammiferes
PL307305A1 (en) * 1993-06-04 1995-05-15 Kwahak International Co Ltd Apparatus for arificial fertilisation and transfer of embryos
US5656010A (en) * 1995-05-09 1997-08-12 The Curators Of The University Of Missouri System for effecting embryo transplant
CA2220550C (en) * 1995-05-09 2001-04-17 Curators Of The University Of Missouri A system for introducing a fluid into the uterus of an animal
US6117068A (en) * 1995-10-19 2000-09-12 Elite Genetics, Inc Artificial insemination system
ATE218051T1 (de) * 1995-12-18 2002-06-15 Mai Genesis Ind Inc Transcervicale infusionspipette
EP2264427B1 (en) 1997-01-31 2017-05-03 Xy, Llc Optical apparatus with focussing reflector for converging radiation onto a flow of particles, and related method of analysis
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
PT1917974E (pt) 1998-07-30 2011-02-22 Xy Llc Sistema de inseminação artificial não cirúrgica em equinos
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
ES2171128B2 (es) * 2000-09-29 2003-11-01 Iberica De Reproduccion Asisti Equipo de inseminacion artificial post cervical en ganado porcino.
DE60014440T2 (de) * 2000-01-03 2005-11-17 Ibérica de Reproducción Asistida, S.L. Vorrichtung zur künstlichen befruchtung von schweinen
IL152714A (en) 2000-05-09 2014-03-31 Xy Llc High-purity spermatozoa populations carrying chromosome-x and chromosome-y
EP1190684A1 (en) 2000-09-05 2002-03-27 Universiteit Gent Device and method for artificial insemination of bovines and other animals
US6511415B1 (en) 2000-11-03 2003-01-28 Continental Plastic Corp. Device for trans-cervical artificial insemination and embryo transfer
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
WO2002043486A1 (en) 2000-11-29 2002-06-06 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7175590B2 (en) 2001-11-01 2007-02-13 Continental Plastic Corp. Apparatus for trans-cervical artificial insemination and embryo transfer
JP4595067B2 (ja) 2002-08-01 2010-12-08 エックスワイ,エルエルシー 低圧精子細胞分離システム
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
CA2534394C (en) 2002-08-15 2013-01-08 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
US8202210B2 (en) 2007-07-27 2012-06-19 Stroud Brad K Artificial breeding techniques for bovines including semen diluents and AI apparatus
US9433484B2 (en) 2007-07-27 2016-09-06 Brad K. Stroud Artificial breeding techniques for bovines including semen diluents and AI apparatus
FR2932977B1 (fr) * 2008-06-30 2012-02-10 Imv Technologies Instrument de transfert intra-uterin par la voie naturelle vagino-uterine
US10610343B2 (en) 2013-07-03 2020-04-07 Brad K. Stroud Method, apparatus and kit for artificial insemination of bovine
JP5689968B2 (ja) 2010-08-10 2015-03-25 ストラウド,ブラツド・ケイ 畜牛の人工授精において使用される精子の数を減少させるための方法および装置
US11622844B2 (en) 2010-08-10 2023-04-11 Maximate, Llc Method, apparatus and kit for artificial insemination of bovine
AU2019333169B2 (en) * 2018-08-31 2022-07-28 Inguran, Llc Devices and methods for artificial insemination
CN109223143B (zh) * 2018-11-09 2021-04-16 王爱华 一种用于试管婴儿的胚胎移植器
CN119970293B (zh) * 2025-04-16 2025-08-26 通辽市农牧业科学院 一种羊育种用胚胎移植枪及移植方法

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FR1014413A (fr) * 1950-03-03 1952-08-14 Pistolet pour l'insémination artificielle et autres opérations d'injection
FR1472139A (fr) * 1965-12-29 1967-03-10 Procédé et appareillage pour la préparation de doses pour l'insémination artificielle et, en particulier, outil formant pince-pistolet utilisable dans ce procédé et pour l'insémination directe de petits animaux
FR2477008A1 (fr) * 1980-03-03 1981-09-04 Cassou Bertrand Appareil d'injection tel que pistolet d'insemination artificielle, notamment pour ovins

Also Published As

Publication number Publication date
ATE11637T1 (de) 1985-02-15
EP0071538A1 (fr) 1983-02-09
DE3262220D1 (en) 1985-03-21
FR2510393B1 (https=) 1983-12-30
FR2510393A1 (fr) 1983-02-04

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