EP0051675A1 - Propagation aseptique serielle de parties de plantes a l'exterieur du sol - Google Patents

Propagation aseptique serielle de parties de plantes a l'exterieur du sol

Info

Publication number
EP0051675A1
EP0051675A1 EP19810901470 EP81901470A EP0051675A1 EP 0051675 A1 EP0051675 A1 EP 0051675A1 EP 19810901470 EP19810901470 EP 19810901470 EP 81901470 A EP81901470 A EP 81901470A EP 0051675 A1 EP0051675 A1 EP 0051675A1
Authority
EP
European Patent Office
Prior art keywords
cultures
plant
culture
plants
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19810901470
Other languages
German (de)
English (en)
Inventor
John E. Staba
Joseph H.C. Lui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Minnesota
Original Assignee
University of Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Minnesota filed Critical University of Minnesota
Publication of EP0051675A1 publication Critical patent/EP0051675A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques

Definitions

  • This invention is directed to methods for the establishment and maintenance of serially propagated cultures of above-ground portions of plants, i.e., stem/leaf or leaf organ cultures.
  • the invention is directed to the method for the establishment and maintenance of serially propagated cultures of the above-ground portions of plants.
  • a culture of an above-ground plant portion is initiated on a ⁇ nutrient plant cell medium containing a growth regulator.
  • the initial culture is maintained under appropriate growth culture conditions after which the culture is transferred to a liquid nutrient plant cell medium containing a growth regulator.
  • the culture is maintained in the liquid medium until the cultures without roots are established. These tures grow rapidly and are serially subcultured in liquid medium containing a growth regulator so long as opti ⁇ mum growth continues.
  • the method of this invention makes possible the direct propagation of original plant organs without embryogenesis or regeneration of a whole plant with
  • a culture of an above-ground plant portion is initiated on a solid plant tissue culture medium con ⁇ taining a growth regulator.
  • the selected above-ground plant portion should be comprised of young vigorous dividing tissue from plants after germination.
  • the plant portion may be seedlings or excised portions of stem/leaf or leaf organs containing meristematic regions of aseptically or non-aseptically grown plants. Non-aseptic plant portions are sterilized before intro ⁇ duction to the medium.
  • a preferred medium is Murashige and Skoog's revised tobacco medium containing agar.
  • This solid medium preferably contains a cytokinin- like growth regulator such as kinetin and/or benzyl- adenine, or the like.
  • a cytokinin- like growth regulator such as kinetin and/or benzyl- adenine, or the like.
  • benzyladenine When benzyladenine is used, it is incorporated into the medium in amount between about 0.05 to 20 pp , and preferably about 2 to 20 ppm. If kinetin is used, the amounts range from about 1 to 100 ppm.
  • the initial culture is maintained under aerobic culture growth conditions at a temperature of about 8° to 30° C for a period of about 3 to 5 weeks.
  • a liquid medium preferably Murashige and Skoog's medium without agar, containing a cytokinin-like growth regulator ' .
  • Benzyladenine may be used in amounts from about 0.05 to 20 ppm, and preferably from about 0.5 to 10 ppm.
  • Kinetin may be used in amounts from about .5 to 200 ppm, or preferably about 5 to 100 ppm.
  • the liquid cultures are shaken gently to introduce air to the medium.
  • the cultures are maintained under appropriate cul ⁇ ture growth conditions at a temperature between about 8° to 30° C and under continuous or intermittent light conditions between about 100 to 10,000 foot candle power.
  • the cultures are maintained under a daylight cycle of about 8 to 20 hours duration and ideally about 16 hours duration.
  • leaf cultures without roots appear in the medium and grow very rapidly.
  • the leaf cultures are serially sub- cultured into liquid medium about every three to twenty weeks. Each subculture will grow very rapidly and is maintained until optimum organ growth ceases.
  • the culture transfers are made under aseptic con ⁇ ditions. Once the above-ground plant cultures are initiated, the new cultures are serially propagated from preexisting cultures without involving regenera ⁇ tion of plant callus or intervention of a whole plant. The generation and propagation of only leaf organ can be achieved in relatively short time and relative- ly minimum effort as compared to field growth of the plant. I_n vitro aseptic serial propagation of above- ground plant portions can be carried out without con ⁇ cern for geographic location or time of year.
  • the method of this invention may be applied to most existing plant species. It is especially adapted to the subculturing of plant species from which useful materials may be extracted, including Digitalis, Dioscorea, Chrysanthemum, Pyrethrum, Catharanthus, Pinus, Papaver, Yucca, Guayule and other latex bearing plants, and the like.
  • Example 1 Digitalis lanata leaf cultures were established from aseptically germinated seeds. Cotyledon seedlings, i.e., those with cotyledons but without true leaves, were
  • Example 2 The establishment of shoot cultures of Dioscorea compositae and their ability to synthesize diosgenin were determined. Young stem segments measuring 3-4 mm and with nodes were collected from greenhouse grown D. compositae plants. The explants were washed with a 1:75.0 dilution of benzalkonium chloride for 3 minutes, washed and surface sterilized with 2 per cent sodium hypochlorite for 3 minutes.
  • the explants were asepti- cally cut at the edges and cultured on Murashige and Skoog's revised tobacco medium containing 1 per cent agar and supplemented with different concentrations (0.1 ppm, 0.5 ppm, 0.75 ppm, and 1.0 ppm of benzyl ⁇ adenine) .
  • the cultures were incubated in vials con ⁇ taining 18 ml of the medium at 24 +_ 2° C and 16 hours of light (750 f.c.) daily. After 2 subcultures of 30 days each, the shoots were transferred to Murashige and Skoog's liquid media with varying concentrations of benzyladenine.
  • the suspension cultures were shaken at 75 rpm at 24 +_ 2° C with 18 hour light periods

Abstract

Procedes permettant l'etablissement et l'entretien de cultures propagees en serie de parties de plantes a l'exterieur du sol, c'est-a-dire, de cultures d'organes de tige/feuille ou de feuille contenant des regions meristematiques des plantes. Une culture d'une partie de plante non enterree est initiee dans un milieu nutritif des cellules de la plante contenant un regulateur de croissance et maintenue dans des conditions appropriees de culture de croissance, apres quoi la culture est transferee dans un milieu nutritif liquide de cellules vegetales contenant un regulateur de croissance. La culture liquide est maintenue jusqu'a l'etablissement de cultures de plante a l'exterieur du sol sans racines dans le milieu. Ces cultures grandissent rapidement et sont sous-cultivees en serie dans un milieu liquide contenant un regulateur de croissance aussi longtemps que la croissance optimale continue. Ce procede peut etre applique a la plupart des especes de plantes existantes independamment de la situation geographique, de l'epoque de l'annee ou analogue.
EP19810901470 1980-05-12 1981-05-11 Propagation aseptique serielle de parties de plantes a l'exterieur du sol Withdrawn EP0051675A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14900980A 1980-05-12 1980-05-12
US149009 1980-05-12

Publications (1)

Publication Number Publication Date
EP0051675A1 true EP0051675A1 (fr) 1982-05-19

Family

ID=22528405

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19810901470 Withdrawn EP0051675A1 (fr) 1980-05-12 1981-05-11 Propagation aseptique serielle de parties de plantes a l'exterieur du sol

Country Status (2)

Country Link
EP (1) EP0051675A1 (fr)
WO (1) WO1981003255A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU183433B (en) * 1981-05-12 1984-05-28 Richter Gedeon Vegyeszet Process for producing multiplying material of digitalis lanata ehrh in tissue culture
HU183978B (en) * 1982-06-28 1984-06-28 Gyogyszerkutato Intezet Process for preparing the propagative material of plants in tissue culture
US4466216A (en) * 1982-07-08 1984-08-21 Stauffer Chemical Company Method for propagating plants from tissue cultures
FR2559349B1 (fr) * 1984-02-09 1986-10-24 Ecole Nale Ing Travaux Agricol Clone de crambe maritime (crambe maritima l.) et procede permettant sa multiplication vegetative par culture in vitro
BRPI0911305B1 (pt) 2008-04-14 2019-07-16 Bridgestone Corporation Processos para recuperar borracha a partir de látex de borracha natural
WO2013134430A1 (fr) 2012-03-06 2013-09-12 Bridgestone Corporation Procédés pour extraire du caoutchouc de plantes différentes de l'hévéa
AU2013262725B2 (en) 2012-05-16 2016-10-06 Bridgestone Corporation Compositions containing purified non-Hevea rubber and related purification methods
EP3546508B1 (fr) 2012-06-18 2022-12-28 Bridgestone Corporation Procédé de désolvantisation de bagasse
EP2861628A4 (fr) 2012-06-18 2016-03-30 Bridgestone Corp Systèmes et procédés de gestion des déchets associés au traitement des buissons de guayule pour extraire le caoutchouc
MX363137B (es) 2012-06-18 2019-03-12 Bridgestone Corp Metodos para incrementar el contenido de caucho extraible de material de planta no hevea.
WO2015038707A1 (fr) 2013-09-11 2015-03-19 Bridgestone Corporation Procédés pour extraire du caoutchouc de matière végétale de tks
US10775105B2 (en) 2018-11-19 2020-09-15 Bridgestone Corporation Methods for the desolventization of bagasse

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE817857A (fr) * 1974-07-19 1974-11-18 Procede de multiplication acceleree de plants de fraisiers par "micropropagation in vitro" et plantules obtenues.
GB1549007A (en) * 1976-07-16 1979-08-01 Nat Seed Dev Org Ltd Propagating woody plant material

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8103255A1 *

Also Published As

Publication number Publication date
WO1981003255A1 (fr) 1981-11-26

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Inventor name: LUI, JOSEPH H.C.

Inventor name: STABA, JOHN E.