ECSP099438A - Beta-mananasa de la broca del café, hypothenemus hampei, y usos de la misma - Google Patents

Beta-mananasa de la broca del café, hypothenemus hampei, y usos de la misma

Info

Publication number
ECSP099438A
ECSP099438A EC2009009438A ECSP099438A ECSP099438A EC SP099438 A ECSP099438 A EC SP099438A EC 2009009438 A EC2009009438 A EC 2009009438A EC SP099438 A ECSP099438 A EC SP099438A EC SP099438 A ECSP099438 A EC SP099438A
Authority
EC
Ecuador
Prior art keywords
present
mananase
protein
isolated
polynucleotide
Prior art date
Application number
EC2009009438A
Other languages
English (en)
Inventor
Zornosa Ricardo Acuna
Jocelyn Rose
Original Assignee
Federacion Nac De Cafeteros De
Cornell Res Foundation Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Federacion Nac De Cafeteros De, Cornell Res Foundation Inc filed Critical Federacion Nac De Cafeteros De
Publication of ECSP099438A publication Critical patent/ECSP099438A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01078—Mannan endo-1,4-beta-mannosidase (3.2.1.78), i.e. endo-beta-mannanase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Curing Cements, Concrete, And Artificial Stone (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Road Paving Structures (AREA)

Abstract

Un aspecto del presente invento está relacionado con una proteína de ?-mananasa aislada la cual tiene una secuencia de aminoácidos que es 90% similar a la secuencia de aminoácidos de la SEQ ID NO:1. El presente invento también se relaciona con un polinucleótido aislado que codifica la proteína de ?-mananasa, y un sistema de expresión aislado y una célula hospedera que contiene el polinucleótido. Otro aspecto del presente invento está relacionado con un método de producir de manera recombinante una proteína de ?-mananasa. Este método comprende proporcionar una célula hospedera que contenga el polinucleótido del presente invento y cultivar la célula hospedera bajo condiciones que sean efectivas para que la célula hospedera exprese la proteína de ?-mananasa. La proteína de ?-mananasa es recuperada. Un aspecto adicional del presente invento está relacionado con un método de degradación de mananos y polisacáridos en material vegetal. Este método comprende proporcionar material vegetal y contactar el material vegetal con la proteína de ?-mananasa del presente invento bajo condiciones efectivas para degradar los mananos y polisacáridos en el material vegetal. El presente invento se relaciona con una secuencia de polinucleótidos aislados la cual codifica una enzima de mananasa involucrada en la hidrólisis de polisacáridos de manano, incluyendo moléculas de manano ramificadas y no ramificadas enlazadas entre sí vía un enlace beta 1,4 glicosilo. El polinucleótido es aislado a partir de un genoma de insecto (broca del café, Hypothenemus hampei).
EC2009009438A 2006-11-21 2009-06-17 Beta-mananasa de la broca del café, hypothenemus hampei, y usos de la misma ECSP099438A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US86670506P 2006-11-21 2006-11-21

Publications (1)

Publication Number Publication Date
ECSP099438A true ECSP099438A (es) 2010-01-29

Family

ID=39430120

Family Applications (1)

Application Number Title Priority Date Filing Date
EC2009009438A ECSP099438A (es) 2006-11-21 2009-06-17 Beta-mananasa de la broca del café, hypothenemus hampei, y usos de la misma

Country Status (11)

Country Link
US (2) US7678556B2 (es)
EP (1) EP2121912B1 (es)
JP (1) JP5123948B2 (es)
AT (1) ATE503832T1 (es)
BR (1) BRPI0719038A2 (es)
CO (1) CO6210758A2 (es)
DE (1) DE602007013614D1 (es)
EC (1) ECSP099438A (es)
ES (1) ES2364097T3 (es)
MX (1) MX2009005326A (es)
WO (1) WO2008062317A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2479462A (en) * 2010-04-09 2011-10-12 Univ Exeter Polysaccharide degrading enzymes from Coleoptera insects
KR101582185B1 (ko) * 2013-11-15 2016-01-06 대한민국 산도변화와 수용성 유기용매에 안정성이 높은 신규 갈락토만난 분해효소 유전자 ｅｍ６과 이로부터 생산한 재조합 효소의 이용
CN111826450B (zh) * 2020-09-04 2021-08-24 中国热带农业科学院香料饮料研究所 一种咖啡果小蠹的特异性ss-coi引物对、鉴定试剂盒和快速鉴定方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI931193A0 (fi) * 1992-05-22 1993-03-17 Valtion Teknillinen Mannanasenzymer, gener som kodar foer dem och foerfaranden foer isoleringav generna samt foerfarande foer blekning av lignocellulosahaltig massa
DK48693D0 (da) * 1993-04-30 1993-04-30 Novo Nordisk As Enzym
US6329191B1 (en) 1993-08-30 2001-12-11 Hawaii Biotechnology Group, Inc. DNA encoding recombinant coffee bean alpha-galactosidase
ES2137277T3 (es) * 1994-04-07 1999-12-16 Nestle Sa Hidrolisis de cafe, con una beta-mananasa inmovilizada.
US5874269A (en) 1995-06-07 1999-02-23 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plant
GB9524752D0 (en) 1995-12-04 1996-02-07 Danisco Modification process
WO1999064619A2 (en) * 1998-06-10 1999-12-16 Novozymes A/S Novel mannanases
WO2000028046A1 (fr) * 1998-11-11 2000-05-18 Societe Des Produits Nestle S.A. Mannanase de coffea arabica
KR100390565B1 (ko) * 2000-06-20 2003-07-07 주식회사 씨티씨바이오 만난아제를 생산하는 신규한 바실러스 속 ｗｌ-1 균주
US7041488B2 (en) * 2001-06-06 2006-05-09 Novozymes A/S Endo-beta-1,4-glucanase from bacillus
KR100560375B1 (ko) 2003-06-26 2006-03-13 주식회사 씨티씨바이오 만난아제를 코딩하는 유전자와 그 형질전환체를 이용하여생산된 재조합 만난아제

Also Published As

Publication number Publication date
BRPI0719038A2 (pt) 2013-11-05
JP2010533476A (ja) 2010-10-28
WO2008062317A3 (en) 2008-11-27
US20100035301A1 (en) 2010-02-11
EP2121912A2 (en) 2009-11-25
US20090117614A1 (en) 2009-05-07
US7670818B1 (en) 2010-03-02
ATE503832T1 (de) 2011-04-15
DE602007013614D1 (de) 2011-05-12
US7678556B2 (en) 2010-03-16
EP2121912B1 (en) 2011-03-30
JP5123948B2 (ja) 2013-01-23
CO6210758A2 (es) 2010-10-20
WO2008062317A4 (en) 2009-02-05
WO2008062317A2 (en) 2008-05-29
ES2364097T3 (es) 2011-08-24
MX2009005326A (es) 2009-08-28

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