GB2479462A - Polysaccharide degrading enzymes from Coleoptera insects - Google Patents

Abstract

Coleoptera degrading enzymes are disclosed with a number of specified sequences. The enzymes include mannan endo-1, 4-0-mannosidase, rhamnogalacturonan lysase, polygalacturonase, endo-(3-1, 4-glucanase, cellulose 1, 4-p-cellobiosidase, polygalacturonanase and pectin methylesterase. Also disclosed are methods of degrading plant material using, micro-organisms or plants comprising, and polynucleotides encoding such enzymes, along with methods of controlling insect pests using the aforementioned plants.

Description

Enzymes

Field of the invention

The invention relates to isolated enzymes useful in the degradation of plant material and in biotechnological processes such as the preparation of biofuels and refining of food products.

Background

Biotechnology is one of the fastest growing industries globally, directly affecting many different areas including food and drink production and the pharmaceutical industry.

Although biotechnology' (or the use of organisms to produce high-value products) is 1 0 effectively an ancient science, established 8000 years ago when the first beer was brewed, in the last 50 years it has become critical to many industries around the globe. The more recent development of the biotechnology sector has been promoted by rapid inter-disciplinary advances in the fields of molecular biology, genetics and analytical chemistry.

Enzymes, as robust and novel catalysts for applications in biotechnology, can be regarded as sustainable alternatives (both economically as well as ecologically) to harsh and toxic chemical catalysts, as they work at moderate temperatures and thereby also reduce energy consumption. At the same time, enzymes can perform reactions difficult to perform chemically, such as the cleavage of water in photosynthesis or N2-fixation. As industrial biotechnology enables industries to deliver novel products, which cannot be produced by conventional industrial methods, or to deliver these products with a reduced economic (and, more importantly, ecological) impact, this need for novel biocatalyst tools will only increase.

Lignocellulose is the major constituent of the cell wall of vascular plants and thereby contains most of the carbon in the planet, lignin being the barrier that protects cellulose and hemicellulose from microbial attack. Although, in nature, an enormous quantity of lignocellulose material exists, only a small fraction has been given any economic value (e.g., in agriculture or in the paper and textile industries) and most is still managed as waste. Promoting the principle of sustainable and environmentally friendly development, lignocellulose can be transformed through tailored biotechnological procedures into value-added natural products for animal feed or biofuel industries. However, the hydrolysis of plant-polysaccharides is hampered by the presence of lignin and the compact architecture of the plant cell wall requires an adequate mixture of enzymes to degrade these into fermentable sugars.

Different microorganisms, including bacteria and fungi, are involved in lignin degradation but only white-rot basidiomycetes have developed strategies to efficiently depolymerise and mineralize this recalcitrant' heteropolymer (Martinez et a!. (1996) Eur. J. Biochem. vol. 237 pp 424-32). These organisms have a nonspecific system, including oxidoreductases, low molecular mass metabolites, dehydrogenases and activated oxygen species, whose concomitant action contributes towards effectively removing the lignin barrier, thus increasing the accessibility of plant carbohydrates. The extracellular oxidoreductases, frequently referred as lignin-modifying enzymes, play an essential role in the process. These enzymes include laccases and peroxidases that catalyze the one-electron oxidation of lignin units making subsequent progress through non-enzymatic reactions possible, leading to the production of bond cleavage products. In addition, oxidases are able to produce extracellular H202, as peroxidase co-substrate, which also participates in lignocellulose degradation, since it is the precursor of hydroxyl radical, the strongest oxidizing agent produced by fungi through the iron-catalyzed Haber-Weiss reaction (Akamatsu et a!. (1990) FEBS Left vol. 269 pp 261-3; Guillen et a!. (2000) Appl. Environ. Microbiol. vol. 66 pp 170-5). On the other hand, the complete hydrolysis of cell wall polysaccharides (cellulose and hemicellulose) to monomeric sugars requires the synergic action of multiple enzymes with different substrate specificity, including a broad number of enzymes in the category of cellulases and xylanases. In both cases, endo-enzymes are required which cleave internally the main chains, whereas exo-enzymes liberating sugars from the ends of the polysaccharide chains and glucosidases or 3-xy1osidases hydrolyze the sugar dimers to glucose or xylose, respectively. Most of these enzymes have been studied in fungi, such as Trichoderma, Aspergillus or Penicillium species and full genomic information about some of them is now available (Aro eta!. (2005) FEMS Microbiol. Rev. vol. 29 pp 7 19-39).

Currently, different commercial enzymatic complexes exist which are a heterogeneous mixture of the different enzymes involved in plant-cell wall degradation, mostly containing cellulases and hemicellulases. These enzyme cocktails' are usually produced by fungi growing on plant cell walls. Their efficiency needs to be improved to obtain good yield in the use of plant biomass as source of fermentable sugars.

Summary of the invention

According to a first aspect of the invention, there is provided a Coleoptera polysaccharide degrading enzyme comprising an amino sequence which is: a) at least about 55% identical to one or more of SEQ ID NOs:67-7 1; b) at least about 45% identical to one or more of SEQ ID NOs: 143-147; c) at least about 40% identical to one or more of SEQ ID NOs:55-61; d) at least about 65% identical to one or more of SEQ IDNOs:1-15; e) at least about 86% identical to one or more of SEQ ID NOs: 16-24; at least about 81% identical to one or more of SEQ ID NOs:26-50 or 52-54; g) at least about 98% identical to one or more of SEQ ID NOs:5 1 or 62-66; or comprising an enzymatically functional fragment or variant of any of these.

It has been found that such polysaccharide degrading enzymes are expressed by the insect order Coleoptera and that the genes encoding the enzymes can be expressed by micro-organisms andlor plants used in biotechnological processes. There is a strong prejudice in the art that, when enzymes are isolated from an insect, they are in reality derived from organisms existing within the insect in a symbiotic relationship. The inventors have surprisingly found that the enzymes can, in fact, be obtained from Coleoptera insects and have been shown to be insect-derived, rather that symbiont-derived. Therefore, the term "Coleoptera polysaccharide degrading enzyme" may indicate that the enzyme is obtainable from one or more Coleoptera insects. The enzyme may be isolated, for example not contained within an insect cell.

With more than one million species (more than 25% of all known species), insects are the most species-rich group on earth and, as such, they exploit every available ecological niche. Insects are both crop pests and important vectors for infectious diseases and, therefore, previous applied entomological research has focused on crop protection and vector control. Tnsects can exploit every foodstuff from plant material to other animals, decaying organic material, blood, stored products and also non-food resources like wood and even buildings. it is estimated that up to half of a given crop can be lost via insect

damage either in the field or upon storage.

Advantageously, a method including the use of such enzymes (as outlined below) widens the range of plant biomass starting materials that may be used in, for example, biofuel production. This enables the use of starting materials which are not also required by humans and animals for food and/or of starting materials which can grow on land and in environments which cannot be used to grow vital food crops. Possible starting materials include non-food crops such as willow (grown in temperate zones), wood from other sources, as well sugar cane (grown in the tropics) and paper. Enzymes derived from insects have not, thus far, been utilised in biotechnological processes. Such utilisation is beneficial because insects are very specialised for their habitat, so that the polysaccharide degrading enzymes expressed by them are particularly efficient for promoting the reactions which they catalyse. This enables biotechnological processes to be operated at, for example, ambient temperature, thus reducing costs. Enzymes derived from organisms such as yeasts and bacteria require temperature to be maintained higher than typical ambient temperature, typically at least 3 7°C.

Uses of the enzymes of the invention include biofuel production, where cellulases are engineered into oily' yeasts to facilitate the breakdown of plant material/biomass for ethanol production. n temperate countries, such as the UK, this can be to process alternative sources of biomass such as willow whilst, in the tropics, this can used for crops such as sugar cane. The enzymes can also be used in a range of applications for the preparation of food, for example the use of pectinases in the clarification of fruit juice or in the treatment of waste water generated by food industries.

The term "polysaccharide degrading enzyme" indicates an enzyme which disrupts any part of a polysaccharide molecule structure and need not be an enzyme which disrupts the glycosidic bonds between saccharide monomers. Therefore, the enzyme may be a cellulase such as endo-3-1,4-glucanase or cellulose 1,4-13-cellobiosidase, a hemicellulase such as mannan endo-1,4--mannosidase, a pectinase such as polygalacturonase, pectin methylesterase or a polysaccharide lyase such as rhamnogalacturonan lyase. A description of the activity of each enzyme and their uses is set out in Table 2, at the end

of the description.

In one embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 55% identical to mannan endo-l,4-13-mannosidase SEQ ID NO:67 and/or one or more of SEQ ID NOs:68-71, as determined by BLAST sequence comparison. For example, the enzyme may have a sequence which is at least about 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% identical to at least one of those sequences. The most closely related sequences identified by BLAST sequence comparison are sequences from molluscs, which are, at most, 40% identical to any of the sequences listed as SEQ ID NOs:67-71. The inventors have found that such enzymes are obtainable from a Callosobruchus species and have an amino acid sequence at least 55% identical to one or more of SEQ ID NO:67-70, or from a Gastrophysa species and have an amino acid sequence at least 55% identical to SEQ ID NO:71. All of the amino acid sequences SEQ ID NOs:67-71 have been found to be at least 55% identical to one another.

Therefore, enzymes exemplified by SEQ ID NOs:67-71 represent a new class of enzymes in Coleoptera, with sequence identity of 55% or more between enzymes derived from different genera. Tt is particularly surprising in view of the fact that the known proteins having closest sequence identity (no more than 40% identical) are found in molluscs, a completely different phylum to Coleoptera.

There are some conserved sequence domains found within the sequences SEQ ID NO:67- 71. For example, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:67: [2431 (G/S)(E)(F/L/W)(T/L)(T/A)(T)(T/N)(V/)(C/S)(E/P)(KIV)(C/T)(R)(D)(HiM)(Y) (SEQ ID NO: 158) In this representation of the sequence, each amino acid position is represented within parentheses, with the amino acids available for selection in that position being separated by a forward slash mark "I". For example, position 1 is G or S, position 2 is always E, position 3 is F, L or W, position 4 is T or L, position 5 is T or A, position 6 is always T and so on. This may be further understood with reference to the accompanying Sequence Listing. The selection of a particular amino acid at any given position is independent of the selection of a particular amino acid at any other position within the conserved sequence. Therefore, the representation of the sequence given above is a disclosure of each and every available combination of amino acids at each position.

Other conserved consensus sequences are disclosed elsewhere in this specification and the preceding explanation of the disclosure applies to the representation of these sequences also.

Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:67: [3111 (E)(F)(S/A)(T)(T/AIC)(N/C/I)(S/N/T)(E)(S/C/L)(H/Q)(S/D/N)(AIP/S) (S/V/A)(W/A/K)(N)(Y)(R/H/Q)(H/Y)(I/L)(Y)(E/D/N)(G1S)(G/D)(F/Y)(G/S/V)(G) (I/A) (L/M)(S/A)(W/F)(Q)(Y)N) (SEQ ID NO: 159) Equivalent sequences may be identified as present in other enzyme sequences using sequence analysis and alignment methods described below, within the routine ability of the skilled person. In an embodiment, an enzyme having at least 55% sequence identity to one or more of SEQ ID NOs:67-71 and comprising one or more of consensus sequences SEQ ID NOs: 158 & 159 falls within the scope of the invention.

th another embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 45% identical to rhamnogalacturonan lyase SEQ ID NO:143 and/or one or more of SEQ ID NOs:144-147, orto one or more of the insect-derived sequences shown in Figure 6, as determined by BLAST sequence comparison.

S For example, the enzyme may have a sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77/, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% identical to at least one of those sequences. Each of sequences SEQ ID NOs:143-147 is no more than 40% identical to published sequences and these sequences share at least 45% sequence identity with one another. Transcripts encoding this rhamnogalacturonan lyase (PL4) were not found in the Rice weevil or in D. abbreviatus and, to date, seem to be restricted to bark beetles only (subfamily Scolytinae). Therefore, enzymes exemplified by SEQ ID NOs: 143-147 represent a new class of enzymes in Coleoptera, with sequence identity of 45% or more between the identified enzymes.

There are some conserved sequence domains found within the sequences SEQ ID NO:143-147. For example, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:143: [2721 (T)(G)(S/T/N/D)(D/N/E)(S/G/E)(AIS/E)(T/V)(L/V/I(S/A)(D/A)(V/A)(A/E/Q) (N/T)(Q/R)(AIV)(A/Q/L/E) (SEQ ID NO: 153) Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:143: [3131 (T/K/N/R)(V/I)(T/S)(G)(Q)(P/K/T)(S/K/L)(A)(T/M/A)(V/I)(M/V)(L/V) (Y/W)(D)(S/T/A) (SEQ ID NO:154) Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:143: [362] (N/R!D)(V/I)(V/A)(A)(Y)(P)(T/L/V)(A)(G)(Q/L/H)(G)(S)(E/D)(N/S)(L/E) (A!D)(E/KIT/R)(S/T/K)(T/S) (SEQ ID NO:155) Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:143: [4551 (A!T/P)(G)(I/S)(W)(T/Y/K)(I/V)(K/Q/E/T)(Y)(Q/E/D)(D)(AIT/E)(X)(D/N)(G) (N/S/A/G)(G/S/AIT)(R)(T/)(L)(R)(V) (SEQ ID NO:156) In this sequence, "X" at position 12 is any amino acid but may be, for example, any one ofK, Q, P,A, RorN.

Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:143: [5181 (S)(N/E)(V/I)(F/Y/1)(T/A)(V/A!I)(T/N/S/P)(N/S/A)(A/N)(Q/Y)(V/L/I)(V/A) (S/Dft) (SEQ ID NO:157) Equivalent sequences may be identified as present in other enzyme sequences using sequence analysis and alignment methods described below, within the routine ability of the skilled person. In an embodiment, an enzyme having at least 45% sequence identity to one or more of SEQ ID NOs:143-147 and comprising one or more of the consensus sequences SEQ ID NOs: 153-157 falls within the scope of the invention.

In another embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 40% identical to polygalacturonase SEQ ID NO:55 and/or one or more of SEQ ID NOs:56-61, as determined by BLAST sequence comparison. For example, the enzyme may have a sequence which is at least about 45%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59/, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74/o, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% identical to at least one of those sequences.

The most closely related sequences identified by BLAST sequence comparison are bacterial sequences which are, at most, 39% identical to any of the sequences listed as SEQ ID NOs:55-61. The inventors have found that such sequences are obtainable from a Callosobruchus species and have an amino acid sequence at least 40% identical to one or more of SEQ ID NO:55-61. All of these amino acid sequences have been found to be at least 40% identical to one another.

Therefore, enzymes exemplified by SEQ ID NOs:55-61 represent a new class of enzymes in Coleoptera, with sequence identity of 40% or more between enzymes. it is particularly surprising in view of the fact that the known proteins having closest sequence identity (no more than 39% identical) are found in bacteria, in a completely different phylogenetic domain to Coleoptera.

There are some conserved domains found within the sequences by SEQ ID NOs:55-61 which are not found in any comparative sequences, appearing as inserts in the representation shown in Figure 3 as compared to the polygalacturonase sequences obtained from a different Coleoptera, C. treinulae. For example, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ifi NO:55: [931 (L/E)(Y)(P)(P)(L/V/F/T)(T/D/H/N)(V/K/A/G)(K!T/L/V)(L)(P)(D/S/N)(G)(X1)(X2) (R!I/F/V)(X3)(L/F/V)(X4)(F/Y)(T) (SEQ ID NO: 160) In this sequence, Xi, X2, X3 and X4 are each any amino acid, with X1 being, for example K, Q, R, L or E, X2 being, for example, R, T, V, K, I or L, X3 being, for example, 5, H, A, N or I and X4 being, for example, P, 5, D, Q or A. Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:55: [2051 (G)(I)(N)(C/V/L)(X1)(S/P)(C/V)(R)(Y/R!Q)(L/V)(H)(IIV)(T/S/K)(G/N)(V/A) (T/D/N)(I/V)(X2)(T/S)(G) (SEQ ID NO:161) In this sequence, X1 and X2 are each any amino acid, with X1 being, for example N, V, D, E or S and X2 being, for example, 5, A, D, H or R. Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:55: [2281 (IIV)(A/V)(L/I/F/M)(D/N/Q/K)(A)(D/WIN/G)(G/L/M/K)(X)(G/L/D/R)(KIRIN) (SEQ ID NO:162) In this sequence, X is any amino acid, for example K, G, R, Q, H or Y. Equivalent sequences may be identified as present in other enzyme sequences using sequence analysis and alignment methods described below, within the routine ability of the skilled person. In an embodiment, an enzyme having at least 40% sequence identity to one or more of SEQ ID NOs:55-61 and comprising one or more of consensus sequences SEQ ID NOs:160-162 falls within the scope of the invention.

In another embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 65% identical to endo-13-1,4-glucanase SEQ ID NO:1 and/or one or more of SEQ ID NOs:2-15, as determined by BLAST sequence comparison. For example, the enzyme may have a sequence which is at least about 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% identical to at least one of those sequences. Related sequences have been identified in fungi and bacteria, but those enzymes possess an additional cellulose binding domain compared to the insect enzymes, positioned at the N-or the C-terminus of the enzymatic domain. Therefore, in this embodiment of the invention, the enzyme may lack a cellulose binding domain. The presence or absence of such a domain can be readily determined by the skilled person, without use of inventive skill. For example, endo-3-1,4-glucanase enzymes in fungi all comprise a cellulose binding domain, for example as seen in CAZy accession no. ADU33286.1.

Advantages are provided by use of these insect enzymes in the industrial context, since fewer resources are required to obtain shorter amino acid sequences, whether synthetically or by use of micro-organisms. In addition, the enzyme may be able to process a wider range of substrates in the absence of a carbohydrate binding domain, since there is a reduced limitation on the types of substrate to which the enzyme can bind.

For example, the enzyme may be obtainable from a Chrysomela species and have an amino acid sequence at least 65% identical to SEQ ID NO: 1 and/or 2. The enzyme may be obtainable from a Gastrophysa species and have an amino acid sequence at least 65% identical to SEQ TD NO:3, or be obtainable from a Leptinotarsa species and have an amino acid sequence at least 65% identical to one or more of SEQ ID NO:4-1O. The enzyme may be obtainable from a Sit oph i/us species and have an amino acid sequence at least 65% identical to one or more of SEQ ID NO: 11-15.

In another embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 86% identical to cellulose 1,4-13-cellobiosidase SEQ ID NO:16 and/or one or more of SEQ lID NOs:17-24 andlor the insect-derived sequences shown in Figure 2, as determined by BLAST sequence comparison. For example, the enzyme may have a sequence which is at least about 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% identical to at least one of those sequences. Related sequences have been identified in bacteria, but those enzymes possess an additional cellulose binding domain compared to the insect enzymes, positioned at the N-terminus of the enzymatic domain. The insect enzymes also have four extra cysteine residues conserved between sequences, as compared with bacterial sequences. For example, in SEQ ID NO:16, these residues are found at positions 181, 213, 299 and 305. Equivalent positions in SEQ ID NOs:17-24 can be determined by sequence alignment using methods described below, within the routine ability of the skilled person. For example, the location of the cysteine residues may be observed in Figure 2. The presence of these residues enables the formation of two additional disulphide bonds, which serve to stabilise the enzyme and improve its activity over a range of temperatures and pH, by reducing the likelihood of denaturation at extremes of temperature and pH. Advantageously, therefore, such an enzyme may be utilised in industrial processes operated at both ambient temperature and at temperatures higher than this, which might be required for other components of the process to work effectively.

For example, the enzyme may be obtainable from a chrysomela species and have an amino acid sequence at least 86% identical to one or more of SEQ ID NOs:16 or 17, or be obtainable from a Gastrophysa species and have an amino acid sequence at least 86% identical to one or more of SEQ ID NO:1 8-20. The enzyme may be obtainable from a Leptinotarsa species and have an amino acid sequence at least 86% identical to one or more of SEQ ID NOs:21-23, or be obtainable from a Sitophilus species and have an amino acid sequence at least 86% identical to SEQ ID NO:24.

For example, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO: 16: [1811 (C)(E/L/Q/S)(L/A!G/D)(G)(X 1)(X2)(A/T/V)X3)(G/K)(P)(S)(F/Y/L)(IiM)N) (T/N/S)(F/Y)(Q/E/G)(R)(G)(P/S)(Q/E/S)(E)( S/N)(V/T)(W)(RIK)(T/A)(TIV)(P)(Q/S) (T/P/G)(T/C/T)(C) (SEQ ID NO:163) In this sequence, X1 is P or no amino acid. X2 and X3 are each any amino acid, with X2 being, for example, 5, Q, Y, G or E and X3 being, for example, 5, D, P. E, K or N. Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:16: [299] (C)(V/I)(G/S/D)(P/T/A)(Y/W/H)(N/S/A/K)(C) (SEQ ID NO: 164) Equivalent sequences may be identified as present in other enzyme sequences using sequence analysis and alignment methods described below, within the routine ability of the skilled person. In an embodiment, an enzyme having at least 86% sequence identity to one or more of SEQ ID NOs:16-24 and comprising one or more of consensus sequences SEQ ID NOs: 163 & 164 falls within the scope of the invention.

In another embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 81% identical to polygalacturonan SEQ ID NO:26 and/or one or more of SEQ ID NOs:27-50 or 52-54, or at least about 98% identical to SEQ ID NO:5 1, as determined by BLAST sequence comparison. For example, the enzyme may have a sequence which is at least about 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or about 100% identical to at least one of SEQ ID NOs:26-50 or 52-54, or at least about 99% or 100% identical to SEQ ID NO:51. For example, the enzyme may be obtainable from a chiysomela species and have an amino acid sequence at least 81% identical to one or more of SEQ ID NOs:26-33, or be obtainable from a Gastrophysa species and have an amino acid sequence at least 81% identical to one or more of SEQ ID NOs:34-40, or be obtainable from a Leptinotarsa species and have an amino acid sequence at least 81% identical to one or more of SEQ ID NOs:41 -50, or be obtainable from a Sitophilus species and have an amino acid sequence at least 98% identical to SEQ ID NO:51 or at least 81% identical to one or more of SEQ ID NOs:52-54. These enzymes all include 10 cysteine residues, compared to 8 cysteine residues in related fungal sequences. For example, the additional residues are found at positions 139 and 143 in SEQ TD NO:26. Equivalent positions in SEQ ID NOs:27-54 can be determined by sequence alignment using methods described below, within the routine ability of the skilled person. For example, the location of the cysteine residues may be observed in Figure 3. Again, the additional disuiphide bonds which can form help to stabilise the enzyme as compared to the fungal equivalents and improve enzyme activity at extremes of temperature and pH. As discussed above, this provides advantages when the enzyme is to be utilised in an industrial process.

For example, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ TD NO:26: [135] (N/F/K)(I/L)(K/L)(N)(C)(P)(X1)(F/H/A/Q)(C)(V)(X2)(I) (SEQ ID NO: 165) In this sequence, X1 and X2 are each any amino acid, with X1 being, for example V, T, H, M or L and X2 being, for example, A, 5, K, I or Y. Equivalent sequences may be identified as present in other enzyme sequences using sequence analysis and alignment methods described below, within the routine ability of the skilled person. In an embodiment, an enzyme having at least 86% sequence identity to one or more of SEQ ID NOs:26-5 1 or 52-54 or 98% sequence identity to SEQ ID NO:5 1 and comprising consensus sequence SEQ ID NOs:165 falls within the scope of the invention.

In another embodiment, the Coleoptera polysaccharide degrading enzyme comprises an amino sequence which is at least about 98% identical to pectin methylesterase SEQ ID NO:62 and/or one or more of SEQ ID NOs:63-66 and/or the insect-derived sequences shown in Figure 4, as determined by BLAST sequence comparison. Such enzymes may be obtainable from an insect in the family Curculionidae such as a Sitophilus or a Dendroctonus species.

There are some conserved domains found within the sequences by SEQ ID NOs:62-66 which are not found in any comparative sequences, appearing as inserts in the representation shown in Figure 4 as compared to the reference sequence. For example, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:62: [33] (E)(A/S)(S/N/A/Q)(X1)(Y/F)(T/I/Q)(E/T/Q)(E/A/T/L)(N/T/E)(Y/V)(L/F)(G/Q) (G/D)(W)(S/L/E/G)(P)(E/P/Q)(S/N/E/L)(I)(X2)(T/L/I/V)(P/S/V)(D/K/Q/E)(X3) (P/A)(D) (Y)(T/S/I)(V)(G/K)(X4)(G) (SEQ ID NO: 166) th this sequence, X is E, V or no amino acid. X1, X2 and X4 are each any amino acid, with Xi being, for example, Y, Q, N, K or R, X2 being, for example, 5, N, H, I or V and X4 being, for example, N, 5, Y, A or V. Alternatively or additionally, the enzyme according to this embodiment of the invention may comprise the following sequence, with the figure in square brackets indicating the position of the first amino acid of the sequence in SEQ ID NO:62: [127] (T/I)(T/H/Q)(G/P/IIA)(X1)(D/A!E/G)(Y)(A!K!M/V)(N/S)(L/T)(V)(X2)(X3)(X4)(G) (E/S/T/A)(R!L/K)(Y/F)(KIQ/N)(S/E/T)(G/A)(D) (SEQ ID NO: 167) In this sequence, Xi is any amino acid, for example, 5, T, I, L or E. X2 is N or no amino acid. X3 is P or no amino acid. X4 is N, E or no amino acid.

Equivalent sequences may be identified as present in other enzyme sequences using sequence analysis and alignment methods described below, within the routine ability of the skilled person. In an embodiment, an enzyme having at least 98% sequence identity to one or more of SEQ ID NOs:62-66 and comprising one or more of consensus sequences SEQ ID NOs: 166 & 167 falls within the scope of the invention.

According to a second aspect of the invention, there is provided a method of degrading plant material comprising exposing the material to at least one polysaccharide degrading enzyme according to the first aspect of the invention, e.g., having an amino acid sequence of an enzyme obtainable from an insect of the order Coleoptera, or an enzymatically functional fragment or variant of such an enzyme. The enzyme used in the method is isolated, in that it is not contained within an insect. Enzymes having an amino acid sequence of an enzyme obtainable from the families Chrysomelidae and Curculionidae are particular examples. A related method may be a method of degrading or processing pectin-containing mixtures such as fruit juices or food industry waste water.

Exposing the plant or other material to the enzyme may involve, for example, directly contacting the plant material with the enzyme by incubating the material in the presence of the enzyme or passing a liquid containing the enzyme over or through the material.

Alternatively or additionally, the method of the invention may comprise a step of expressing the enzyme in a micro-Organism, such as a bacterium or a yeast such as an oleaginous yeast strain used in the production of biofuels. Suitable micro-organisms are further described below.

The plant or other material may, for example, be contacted with the micro-organism which may secrete the enzyme so that it contacts the plant material and degrades it.

Alternatively or additionally, an extracellular growth medium of the micro-organism may be used to contact the plant material, if the micro-organism secretes the enzyme. The micro-organism may be engineered to include proteins necessary to allow secretion of the enzyme. In a further alternative (which may also be used in addition to the previous options), the plant material may be contacted with an extract of the micro-organism, for example if the enzyme is not secreted by the micro-organism so that disruption of the micro-organism (for example, by cell lysis) is required to obtain the enzyme. In a still further alternative, a plant may itself express the enzyme (for example, by expression of a recombinant gene) which may, therefore, be available for the autodegradation of plant material containing such a plant. This may be by release of the enzyme when the plant is harvested and/or after crushing, shredding or milling of the plant material. Alternatively or additionally, the enzyme may be expressed in the cytosol and be secreted from the cell into the cell surroundings, from where it can act to degrade the plant material. Such expression may be controllable using other elements, for example temperature and/or pH-dependent factors which may "switch on" expression of the enzyme at an appropriate time during processing of the plant material.

In the method according to the second aspect of the invention, the amino acid sequence of the polysaccharide degrading enzyme may correspond to any of those having GenBank Accession Numbers CAA76931.l, AAN78326.l, AAU44973.1, AAR22385.l, BAE94320.l, BAE94321.l, CAH25542.l, CAH25543.l, CAA76930.l, ACP18831.l (SEQ IDNO:25),AAG35693.l orAAW28928.l.

In the method according to the second aspect of the invention, the amino acid sequence of the polysaccharide degrading enzyme may be encoded by and expressed from any of polynucleotide sequences SEQ ID NOs:72-142 or 148-152 or a combination of any of these. These polynucleotide sequences encode for the amino acid sequences SEQ TD NOs:1-71 and 143-147, respectively, as shown in Table 1 below.

According to a third aspect of the invention, there is provided a micro-organism comprising at least one polysaccharide degrading enzyme having (i.e., comprising or consisting of) an amino acid sequence of an enzyme according to the first aspect of the invention, for example, obtainable from an insect of the order Coleoptera, and/or of an enzymatically functional fragment or variant of such an enzyme and/or a polynucleotide coding for such an enzyme and/or for an enzymatically functional fragment or variant of such an enzyme. The enzyme may have an amino acid sequence comprising at least one of SEQ ID NOs:1-71 or 143-147, or comprising at least one amino acid sequence at least about 65% identical to one or more of SEQ ID NOs:1-15, or at least about 86% identical to one or more of SEQ D NOs:16-24 (or any of the insect-derived sequences shown in Figure 2), or at least about 81% identical to one or more of SEQ TD NOs:26-50 or 52-54, or at least about 40% identical to one or more of SEQ ID NOs:55-61, or at least about 98% identical to one or more of SEQ ID NOs:51 or 62-66 (or any of the insect-derived sequences shown in Figure 4), or at least about 55% identical to one or more of SEQ ID NOs:67-71, or at least about 45% identical to one or more of SEQ ID NOs: 143-147 (or any of the insect-derived sequences shown in Figure 6). The micro-organism may alternatively or additionally comprise at least one of the polynucleotide sequences SEQ ID NOs:72-142 or 148-52 or a micro-organism equivalent thereof. The term "micro-organism equivalent" of a polynucleotide sequence SEQ ID NOs:72-142 or 148-152 means a polynucleotide sequence which has been codon optimised so as to enable expression of an insect polynucleotide sequence in a micro-organism host, for example a bacterium or a yeast cell. Such codon optimisation is well understood by the skilled person and "codon" tables are available, for example, online. An example of a yeast codon optimisation table can be seen at www.yeastgenome.org/community-codonusage.shtml (accessed on 7 April 2011). Codon usage in the beetle species described herein is shown in Tables 3 and 4 below which, therefore, provides sufficient information to enable the skilled person to target specific codons which might be altered in a polynucleotide sequence to enable optimal expression in a yeast or other micro-organism.

The micro-organism may be, for example, a bacterium, a fringus, a protist, an alga or an archaeon, for example, a bacterium or a yeast such as an oleaginous yeast strain used in the production of biofuels. The micro-organism may be a strain of fungus, yeast or other micro-organism comprising at least one enzyme according to the first aspect of the invention and at least one enzyme natural to the micro-organism, i.e., present prior to the introduction of the enzyme according to the first aspect of the invention and/or a polynucleotide encoding such an enzyme. An example of a suitable micro-organism is Chrysosporium lucknowense available from Dyadic International, Inc. (Florida, USA).

According to a fourth aspect of the invention, there is provided a plant comprising at least one polysaccharide degrading enzyme having (i.e., comprising or consisting of) an amino acid sequence of an enzyme according to the first aspect of the invention, for example, obtainable from an insect of the order Coleoptera and/or of an enzymatically functional fragment or variant of such an enzyme and/or a polynucleotide coding for such an enzyme and/or for an enzymatically functional fragment or variant of such an enzyme.

The enzyme may have an amino acid sequence comprising at least one of SEQ ID NOs: 1- 71 or 143-147, or comprising at least one amino acid sequence at least about 65% identical to one or more of SEQ ID NOs:1-15, or at least about 86% identical to one or more of SEQ ID NOs:16-24 (or any of the insect-derived sequences shown in Figure 2), or at least about 81% identical to one or more of SEQ ID NOs:26-50 or 52-54, or at least about 40% identical to one or more of SEQ ID NOs:55-61, or at least about 98% identical to one or more of SEQ D NOs:5 1 or 62-66 (or any of the insect-derived sequences shown in Figure 4), or at least about 55% identical to one or more of SEQ D NOs:67-71, or at least 45% identical to one or more of SEQ ID NOs:143-147 (or any of the insect-derived sequences shown in Figure 6).

The plant may comprise at least one of the polynucleotide sequences SEQ ID NOs:72- 142 or 148-152 or a plant equivalent thereof. The term "plant equivalent" of a polynucleotide sequence SEQ ID NOs:72-l42 or 148-152 means a polynucleotide sequence which has been codon optimised so as to enable expression of an insect polynucleotide sequence in a plant host.

a plant, the enzyme may be expressed by all cells of the plant, or by cells in a limited part of the plant, for example, in a root, flower, boll or fruit, or within a subcellular compartment such as a chloroplast, mitochondrion and/or peroxisome. The plant may be, for example, sugar cane or willow.

Advantageously, plants containing such polypeptides and/or polynucleotides may facilitate autodegradation of plant material containing the plant. For example, a polysaccharide degrading enzyme may be released by crushing, shredding and/or milling of the plant, or by secretion of the enzyme from the plant.

In addition, such a plant facilitates control of insect pests using RNAi technology. The plant may comprise a polynucleotide sequence SEQ ID NOs:72-142 or 148-152, a complementary sequence thereof or a portion of any of these, the polynucleotide being a DNA molecule (capable of transcription into an RNA molecule) or an RNA molecule which is useful as an RNAi molecule. Any portion of any of the polynucleotide sequences may be used, with preferred suitable portions being 80-250bp, for example 100-200bp, 120-l8Obp, 130-l7Obp, or about 9Obp, lOObp, l2Obp, l4Obp, l6Obp, l8Obp, 1 9Obp or about 200bp in length. The term "RNAi molecule" indicates a double stranded RINA molecule which, when introduced into a cell (for example, via ingestion by an insect of a plant containing the RINA) activates the RNA interference mechanisms of the cell. Expression of the gene encoding the enzyme corresponding to the polynucleotide sequence may then be reduced or "knocked-down". Baum et a!. (Nature Biotechnology (2007) vol. 25 pp 1322-1326) have shown that RINAi can be used to control insect pests.

It is not necessary to kill an insect to control it, as suggested by the current widespread use of fast acting, small molecule insecticides, which largely act on the insect's nervous system. In fact, it may be desirable to simply slow its growth to avoid feeding damage or the future ability of the insect to reproduce. More specifically, if the fitness' of the insect is reduced, the plant may be able to compensate for the damage and thereby grow out of it. Baum et at. showed that the corn rootworms of their study were not killed, but their feeding capacity was so reduced that the corn plants simply grew more roots. Therefore, damage does not reach economically important levels (where the plants fall over or lodge' as their roots are impaired).

According to a fifth aspect of the invention, there is provided a polynucleotide encoding a polypeptide according to a first aspect of the invention. For example, the polynucleotide may encode one or more of the following polypeptides: a) a polypeptide at least about 55% identical to one or more of SEQ TD NOs:67-71; b) a polypeptide at least about 45% identical to one or more of SEQ TD NOs:143-147; c) a polypeptide at least about 40% identical to one or more of SEQ ID NOs:55-61; d) a polypeptide at least about 65% identical to one or more of SEQ ID NOs:1-15; e) a polypeptide at least about 86% identical to one or more of SEQ ID NOs:16-24; f) a polypeptide at least about 81% identical to one or more of SEQ ID NOs:26-50 or 52-54; g) a polypeptide at least about 98% identical to one or more of SEQ ID NOs:5 1 or 62-66; or an enzymatically functional fragment or variant of any of these For example, the polynucleotide may comprise at least one of SEQ ID NOs: 72-142 or 148-152, which encode for amino acid sequences 1-71 and 143-147 respectively, as shown in Table 1 below. The polynucleotide may form part of an expression vector in which the polynucleotide is operably linked to one or more expression control sequences (e.g., a promoter sequence), so as to be useful to express a polypeptide having any of SEQ ID NOs:1-71 or 143-147 in a host cell such as a micro-organism, plant or insect cell.

The present invention also encompasses structural variants of the polypeptides as defined herein. As used herein, a "variant" means a polypeptide in which the amino acid sequence differs from the base sequence from which it is derived in that one or more amino acids within the sequence are substituted for other amino acids. Amino acid substitutions may be regarded as "conservative" where an amino acid is replaced with a different amino acid with broadly similar properties. Non-conservative substitutions are where amino acids are replaced with amino acids of a different type.

Table 1 showing how the amino acid sequences are encoded by the nucleic acid sequences Amino acid Encoded Amino acid Encoded Amino acid Encoded sequence by sequence by sequence by (SEQ ID NO) (SEQ ID (SEQ ID NO) (SEQ ID (SEQ ID (SEQ ID _________ NO) _________ NO) NO) NO) 1 72 27 98 53 124 2 73 28 99 54 125 3 74 29 100 55 126 4 75 30 101 56 127 76 31 102 57 128 6 77 32 103 58 129 7 78 33 104 59 130 8 79 34 105 60 131 9 80 35 106 61 132 81 36 107 62 133 11 82 37 108 63 134 12 83 38 109 64 135 13 84 39 110 65 136 14 85 40 111 66 137 86 41 112 67 138 16 87 42 113 68 139 17 88 43 114 69 140 18 89 44 115 70 141 19 90 45 116 71 142 91 46 117 143 148 21 92 47 118 144 149 22 93 48 119 145 150 23 94 49 120 146 151 24 95 50 121 147 152 96 51 122 ___________ _________ 26 97 52 123 ___________ _________ By "conservative substitution" is meant the substitution of an amino acid by another amino acid of the same class, in which the classes are defined as follows: Class Amino acid examples Nonpolar: A, V, L, I, P, M, F, W Uncharged polar: G, S, T, C, Y, N, Q Acidic: D, E Basic: K,R.H.

As is well known to those skilled in the art, altering the primary structure of a peptide by a conservative substitution may not significantly alter the activity of that peptide because the side-chain of the amino acid which is inserted into the sequence may be able to form similar bonds and contacts as the side chain of the amino acid which has been substituted out. This is so even when the substitution is in a region which is critical in determining the peptide's conformation.

In the present invention, non-conservative substitutions are possible provided that these do not inteffupt with the enzymatic function of the polypeptides.

Broadly speaking, fewer non-conservative substitutions will be possible without altering the biological activity of the polypeptides. Determination of the effect of any substitution (and, indeed, of any amino acid deletion or insertion) is wholly within the routine capabilities of the skilled person, who can readily determine whether a variant polypeptide retains the DNA polymerase activity according to the invention. For example, when determining whether a variant of the polypeptide falls within the scope of the invention (i.e., is an "enzymatically functional variant"), the skilled person will determine whether the variant retains enzyme activity (i.e., polysaccharide degrading activity) which is at least about 60%, preferably at least about 70%, more preferably at least about 80%, yet more preferably about 90%, about 95%, about 96%, about 97%, about 98%, about 99% or about 100% the activity of the non-variant polypeptide. In some cases, the variant may have enzyme activity which is greater than 100% the activity of the non-variant polypeptide, i.e., the variant may have improved enzyme activity compared to the non-variant. A variant may also be a fragment of the whole polypeptide, i.e., a fragment which retains enzyme activity which is at least about 60% the activity of the full length polypeptide. All such variants are within the scope of the invention.

Activity may be measured by, for example, any standard measure such as determining catalytic activity against a range of commercially available standard substrates such as carboxymethyl-cellulose or crystalline cellulose (for cellulases), pectin (for pectin methylesterases and rhamnogalacturonan lyases), pectic acid (for polygalacturonases) or mannan (for mannan endo-1,4-13-mannosidases). For example, the time required. under comparable conditions, to degrade a certain mass of plant material by a certain amount may be determined as a measure of the level of activity.

Using the standard genetic code, further nucleic acids encoding the polypeptides may readily be conceived and manufactured by the skilled person. The nucleic acid may be DNA or RNA, and where it is a DNA molecule, it may for example comprise a cDNA or genomic DNA.

The invention encompasses variant nucleic acids encoding the polypeptides of the invention. The term "variant" in relation to a nucleic acid sequence means any substitution of, variation of, modification of, replacement of, deletion of, or addition of one or more nucleic acid(s) from or to a polynucleotide sequence, providing the resultant polypeptide sequence encoded by the polynucleotide exhibits at least the same properties as the polypeptide encoded by the basic sequence. The term therefore includes allelic variants and also includes a polynucleotide (a "probe sequence") which substantially hybridises to the polynucleotide sequence of the present invention. Such hybridisation may occur at or between low and high stringency conditions. In general terms, low stringency conditions can be defined as hybridisation in which the washing step takes place in a 0.330-0.825 M NaC1 buffer solution at a temperature of about 40-48°C below the calculated or actual melting temperature (Tm) of the probe sequence (for example, about ambient laboratory temperature to about 55°C), while high stringency conditions involve a wash in a 0.0 165-0.0330 M NaCI buffer solution at a temperature of about 5- 10°C below the calculated or actual Tm of the probe sequence (for example, about 65°C).

The buffer solution may, for example, be SSC buffer (0.15M NaC1 and 0.015M tn-sodium citrate), with the low stringency wash taking place in 3 x SSC buffer and the high stringency wash taking place in 0.1 x SSC buffer. Steps involved in hybridisation of nucleic acid sequences have been described for example in Sambrook et at. (1989; Molecular Cloning, Cold Spring Harbor Laboratory Press, Cold Spring Harbor).

Typically, variants have about 55% or more of the nucleotides in common with the nucleic acid sequence of the present invention, more typically 60%, 65%, 70%, 80%, 85%, or even 90%, 95%, 98% or 99% or greater sequence identity.

Variant nucleic acids of the invention may be codon-optimised for expression in a particular host cell, as mentioned above in relation to the second aspect of the invention.

Enzymes according to aspects of the invention may be prepared synthetically using conventional synthesisers. Alternatively, they may be produced using recombinant DNA technology or be isolated from natural sources followed by any chemical modification, if required. In these cases, a nucleic acid encoding the chimeric protein is incorporated into a suitable expression vector, which is then used to transform a suitable host cell, such as a prokaryotic cell such as E. co/i. The transformed host cells are cultured and the protein isolated therefrom. Vectors, cells and methods of this type form further aspects of the present invention.

Sequence identity between nucleotide and amino acid sequences can be determined by comparing an alignment of the sequences. When an equivalent position in the compared sequences is occupied by the same amino acid or base, then the molecules are identical at that position. Scoring an alignment as a percentage of identity is a function of the number of identical amino acids or bases at positions shared by the compared sequences. When comparing sequences, optimal alignments may require gaps to be introduced into one or more of the sequences, to take into consideration possible insertions and/or deletions in the sequences. Sequence comparison methods may employ gap penalties so that, for the same number of identical molecules in sequences being compared, a sequence alignment with as few gaps as possible, reflecting higher relatedness between the two compared sequences, will achieve a higher score than one with many gaps. Calculation of maximum percent identity involves the production of an optimal alignment, taking into consideration gap penalties.

Suitable computer programs for caffying out sequence comparisons are widely available in the commercial and public sector. Examples include the FASTA program (Pearson & Lipman, 1988, Proc. Natl. Acad. Sci. USA vol. 85 pp 2444-2448; Altschul et al., 1990, J. Mol. Biol. vol. 215 pp 403-410), ggsearch (part of the FASTA package) (Needleman & Wunsch, 1970, J. Mol. Biol. 48: 443-453), and the BLAST software. The latter is publicly available at http://blast.ncbi.nlm.nih.gov/Blast.cgi (accessed on 7 April 2011) and sequence comparisons and percentage identities mentioned in this specification have been determined using this software. The FASTA program can be accessed publicly from the European Bioinformatics hstitute (http://www.ebi.ac.uk!fasta) (accessed on 7 April 2011). Typically, default parameters set by the computer programs should be used when S comparing sequences. The default parameters may change depending on the type and length of sequences being compared. A comparison using the FASTA program may use default parameters of Ktup = 2, Scoring matrix = Blosum5O, gap = -10 and ext -2. A comparison using the BLAST software may use the default parameters (scoring matrix = Blosum62, gap = 11 and ext = 1). As an alternative, the percentage sequence identity may be determined using the MatGAT v2.03 computer software, available from the website http://bitincka.comlledion!matgat/ (accessed on 7 April 2011). The parameters are set at Scoring Matrix = BlosumSO, First Gap = 16, Extending Gap = 4 for DNA, and Scoring Matrix = Blosum62, First Gap 12, Extending Gap = 2 for protein.

According to an sixth aspect of the invention, there is provided a method of controlling an insect pest comprising feeding to the insect a plant according to the fourth aspect of the invention, the plant comprising a polynucleotide coding for an enzyme according to the first aspect of the invention, for example, obtainable from an insect of the order Coleoptera or coding for a portion of such an enzyme. The insect is preferably of the order Coleoptera such as, by way of non-limiting example, Colorado Potato Beetle, Corn Rootworm, Coffee Berry Borer. "Controlling" the insect pest may comprise causing the death of the insect or causing reduced growth, mobility or reproductive capability (e.g., the ability to reproduce or reducing the number of progeny per generation) of the insect, or reversed or arrested development of the insect (since some insects cease to change between instars when starved, remaining as non-reproducing juveniles). The polynucleotide may be any of those disclosed in the fifth aspect of the invention, or a portion of any such polynucleotide. The polynucleotide is preferably an RNA molecule which is useful as an RNAi molecule. Any portion of the polynucleotide sequences may be used, with preferred suitable portions being 80-250bp, for example 100-200bp, 120-l8Obp, 130-l7Obp, or about 9Obp, lOObp, l2Obp, l4Obp, l6Obp, l8Obp, l9Obp or about 200bp in length. Preferably, a process of RNA interference occurs in the insect after the insect has ingested the plant, double-stranded RNA coding for an enzyme obtainable from an insect of the order Coleoptera or coding for a portion of such an enzyme having been expressed in the plant.

Throughout the description and claims of this specification, the words "comprise" and "contain" and variations of the words, for example "comprising" and "comprises", mean "including but not limited to" and do not exclude other moieties, additives, components, integers or steps. Throughout the description and claims of this specification, the singular encompasses the plural unless the context otherwise requires. In particular, where the indefinite article is used, the specification is to be understood as contemplating plurality as well as singularity, unless the context requires otherwise.

Preferred features of each aspect of the invention may be as described in connection with any of the other aspects.

Other features of the present invention will become apparent from the following examples. Generally speaking, the invention extends to any novel one, or any novel combination, of the features disclosed in this specification (including the accompanying claims and drawings). Thus, features, integers, characteristics, compounds or chemical moieties described in conjunction with a particular aspect, embodiment or example of the invention are to be understood to be applicable to any other aspect, embodiment or example described herein, unless incompatible therewith.

Moreover, unless stated otherwise, any feature disclosed herein may be replaced by an alternative feature serving the same or a similar purpose.

Brief description of Figures

Embodiments of the invention will now be described, by way of example only, with reference to Figures 1-6 in which: Figure 1 shows the amino acid alignment of endo-J3-1,4-glucanase (GH45) enzymes from beetles, with the amino acid sequence of the enzymatic domain of the endoglucanase from Humicola insolens used as a reference sequence (not including the cellulose binding domain); conserved amino acids are highlighted dark grey, partially conserved amino acids highlighted pale grey and catalytic residues (according to H. insolens) marked *; Figure 2 shows the amino acid alignment of cellulose 1,4-13-cellobiosidase (GH48) enzymes from beetles, with the amino acid sequence of the enzymatic domain of the cellulose Ce1148F from Clostridium cellulolyticum used as a reference sequence (not including the cellulose binding domain); conserved amino acids are highlighted dark grey, partially conserved amino acids highlighted pale grey and catalytic residues (according to C cellulolyticum) marked; Figure 3 shows the amino acid alignment of polygalacturonase (GH28) enzymes from C. tremulue and C. maculatus, with the amino acid sequence of the endopolygalacturonase II from Aspergillus niger used as a reference sequence; conserved amino acids are highlighted dark grey, partially conserved amino acids highlighted pale grey and catalytic residues (according to A. niger) marked *; Figure 4 shows the amino acid alignment of pectin methylesterase (CE8) enzymes from S. oryzae, with the amino acid sequence of the pectin methylesterase from Erwinia chrysanthemi used as a reference sequence; conserved amino acids are highlighted dark grey, partially conserved amino acids highlighted pale grey and catalytic residues (according to E. chrysanthemi) marked 1'; Figure 5 shows the amino acid alignment of mannan endo-1,4-13-mannosidase (GH5) enzymes from beetles, with the amino acid sequence of the 3-mannanase from the blue mussel Mytilus edulis used as a reference sequence; conserved amino acids are highlighted dark grey, partially conserved amino acids highlighted pale grey and catalytic residues (according to Al. edulis) marked *; and Figure 6 shows the amino acid alignment of rhamnogalacturonan lyase (PL4) enzymes from beetles, with the amino acid sequence of the RhiE protein from the Erwinia chrysanthemi bacterium used as a reference sequence; conserved amino acids are highlighted dark grey and partially conserved amino acids highlighted pale grey.

Examples

insect selection Six key species from within the two groups chiysomelidae (leaf beetles) and Curculionidae (weevils) were selected. Species were chosen based upon pest status and differences in their feeding behaviour (e.g., fresh plant tissue versus stored product feeder). The selected species are: (1) The Poplar leaf beetle, Chrysomela tremulae (Chrysornelidae); (2) The Green dock beetle, Gastrophysa viridula (Chrysomelidae); (3) The Colorado potato beetle, Leptinotarsa decemlineata (Chiysoinelidae); (4) The Cowpea bruchid, Callosobruchus inaculatus (Chrysomelidae); (5) The Rice weevil, Sitophilus oryzae (Curculionidae); and (6) The Mountain Pine Beetle, Dendroctonusponderosae (Curculionidae) 454 sequencing of beetle midgut transcriptomes Actively feeding late instar larvae were dissected from all six beetle species. In the case of the large leaf feeding beetles, this was performed on larvae collected from laboratory cultures. For the insects whose larvae feed inside of pulses or grains, the grains were physically cracked and sieved to remove actively feeding larvae which were then dissected, midguts washed and stored at -80°C before RNA isolation. To prevent over-representation of the most common transcripts and, therefore, to enhance the overall rate of gene discovery, the resulting double-stranded cDNAs were normalised' using the Kamchatka crab duplex-specific nuclease method (Zhulidov et al. Nucl. Ac. Res. (2004) vol. 32 e37).

Trimming and assembly of the raw sequence data was achieved using est2assembly' (Papanicolaou et al. BMC Bioinformatics (2010) vol. 10:447). This program trims sequencing adaptors from the raw sequence reads and then assembles them using two different assembly algorithms (NEWBLER and MIRA2 assemblers) using a variety of assembly parameters. The best' (optimal) assembly can be visually assessed from a number of graphical print outs which examine the total number of nucleotides sequenced, the average length of the contigs, the number of reads per contig and the number of unincorporated reads or singletons'. Tn this work, the assemblies that gave the best' (deepest and most even) coverage of the target enzyme encoding genes and those which leave fewest orphaned singletons were used.

Identification. RACE-PCR and full length Sanger sequencing Following bioinformatics analysis, sequences related to plant cell wall degrading enzyme cDNAs were retrieved from each 454-derived EST dataset via BlastX searches and also detailed visual examinations of potential enzyme active sites. Full-length cDNA sequences were obtained using classical molecular techniques such as Rapid amplification of cDNA ends' or RACE PCR. To exclude the possibility that candidate genes came from microorganisms associated with the beetle's midgut flora (which should have been largely washed away) or actually infecting the midgut tissue, specific primers from each cDNA sequence were designed and used in PCR experiments using, as a template, genomic DNA prepared from a tissue which is not the midgut (legs for example). The presence of a PCR product indicates that these genes truly belong to the insect genome and were not derived from a contaminating microorganism.

Figures 1-6 show amino acid sequence alignments of each class of enzyme, using known sequences for which the crystal structure has been resolved as a reference. In Figure 1, the reference sequence is the endoglucanase from Humicola inso lens (Davies et at.

(1995) Biochemistry vol. 34 ppl62lO-l6220), not including the additional cellulose binding domain lacking in beetle enzymes; in Figure 2 it is the cellulose CeII48F from Clostridium cellulolyticum (Parsiegla et al. (2008) J. Mol. Biol. vol. 375 pp 499-5 10), not including the additional cellulose binding domain lacking in beetle enzymes; in Figure 3 it is the endopolygalacturonase II from Aspergillus niger (van Santen et al. (1999) J. Biol.

Chem. vol. 274 pp 30474-30480); in Figure 4 it is the pectin methylesterase from Erwinia chiysanthemi (Fries et at. (2007) EMBO J. vol. 26 pp 3879-3887); in Figure 5 it is the 3-mannanase from the blue mussel Mytitus edutis (Larsson et at. (2006) J. Mol. Biol. vol. 357 pp 1500-1510); and in Figure 6 it is the RhiE protein, a rhamnose-induced protein from the plant bacterial pathogen Erwin Ia chrysanthemi (Laatu & Condemine (2003) J. Bacteriol. vol. 185 pp 1642-1649). In each case, to facilitate the alignment, the predicted amino-terminal signal peptide of each beetle enzyme (included in SEQ ID NOs:1-71 and 143-147) has been removed. These figures show the sequence conservation of the enzymes between different types of beetle.

Functional expression and enzyme characterisation Following the RACE-PCR or full length candidate genes, all of the genes are cloned into shuttle vectors (such as pCR4-TOPO/TA) in E. co/i. Such E. co/i vectors facilitate the transfer of beetle cDNAs to either yeast or into insect cells. Enzymatic activities associated with lysates of E. co/i are analysed. The beetle enzymes will achieve the correct post-translational processing in insect cells or yeast, therefore giving a more accurate picture of their catalytic activities and efficiencies.

For expression in the insect derived cell line Sf9, the pIB TOPO/TA expression vector (Tnvitrogen) is used. This vector adds a specific epitope tag (V5) at the carboxyl-terminus of each protein. Successful expression of each recombinant protein is assessed by Western blot using a specific antibody directed against the VS epitope, thus avoiding the generation of specific antibodies for each recombinant protein. The predicted protein primary structures corresponding to two endo-13-1,4-glucanases, two cellulose 1,4-13-cellobiosidases and nine endopolygalacturonases cloned from C. tremu/ae midguts, show the presence of an amino-terminal signal peptide. This indicates that these proteins are secreted in the extra-cellular space by midgut cells. The recombinant proteins expressed in Sf9 cells are also secreted into the culture medium.

The presence or absence of enzymatic activity is assessed for each recombinant protein by zymogram analysis, i.e., direct visualisation of the enzymatic activity after protein electrophoresis performed under non-reducing conditions, as well as by classical enzymatic activity assays. Specific substrates are used, e.g., carboxymethyl-cellulose (CMC) as well as microcrystalline cellulose (MCC) for cellulases (endo-13-1,4-glucanases and cellulosc 1,4-13-cellobiosidasc), pcctic acid for endopolygalacturonases, estcrificd pectin for pectin methylesterases and mannan for endo-1,4-13-mannosidases.

RNAi-facilitated control of insect growth Double stranded RNA products encoding plant cell wall degrading enzymes and portions of these can be introduced into insects by (a) direct injection of dsRNA into the insect hemocoel (open blood system); (b) feeding of the insects on the dsRNA by direct application to their food or via expression of dsRNA constructs in plants; and/or (c) expression of the constructs within the genome of the insect via germline mediated transformation, to provide proof of principle for knock-down of enzyme expression by RNAi.

Sections of the genes encoding for the polysaccharide degrading enzymes can be expressed in plants in constructs such as those disclosed in Li et at. (Plant Cell Rep.

(2010) vol. 29 pp 113-123). Expression can be carried out in the tissues where the pest of interest is typically situated, e.g., roots for rootworm, or in given organelles if necessary, e.g., plant chioroplasts. Insects are weighed at different times after exposure to plants with and without RINAi constructs, or with and without direct exposure to dsRINA.

Reduction in weight is recorded relative to the control. A repeatable reduction results in a reduction in the effective feeding damage which can be caused by the pest, or affests development of the pest and/or its ability to reproduce (or reduces the numbers of its offspring). This is, therefore, a controllamelioration strategy for pest damage.

Codon usage in beetle species A meta-analysis of all the publicly available coleopteran EST datasets present in the dbEST database at NCBI was performed. The codon usage of the 167 plant cell wall degrading enzyme messages identified was compared to the one from the overall ESldatasets of each of the beetle species surveyed, as well as selected organisms such as Nosema born bycis, Wolbachia and Saccharomyces cerevisiae. The results are shown in Tables 3 and 4, with Table 3 showing the usage as a fraction of the total and Table 4 showing the usage according to relative synonymous codon usage (RSCU) values. RSCU values lower than 1 indicate that a codon is avoided and values higher than 1 indicate the given codon is prefeffed. A higher RSCU value indicates a higher preference for the particular codon. In both Tables, the preferred codon(s) for each amino acid is highlighted in italics.

Table 2: Description of enzymes according to invention I CAZy Enzyme name E.C. number classcationb i Enzymatic activity Uses Cellulolytic enzymes: ______________ __________________ Fermentation of biomass Endo1L1 4-iucanase Endohydrolysis of (1 -4)-D-D-into biofuels; food SE ID NOw 11 5\ EC 3.2.1.4 GH45 glucosidic linkages in cellulose, processing; laundry * / lichenin and cereal 3-D-glucans. detergents; pulp and paper _________________________ ______________ __________________ industry.

Hydrolysis of (I -4)-3-D-cellulose 1,4-f3-glucosidic linkages in ceElulose and As for GH45; also used in cellobiosidase EC 3.2.1.91 GH48 cellotetraose, releasing cellobiose combination with GH45 (SEQ ID NOs: 16-24) from the non-reducing ends of the enzymes. U.) chains.

Hemicellulolytic enzymes: ______________ __________________ mannan endo-I,4f3-Random hydrolysis of (1 -4)-D-D-As for GH45; also used in mannosidase EC 3.2.1.78 GH5 mannosidic linkages in mannans, combination with (SEQ ID NOs:67-7 1) galactomannans and glucomannans. cellulolytic enzymes.

Table 2: Description of enzymes according to invention Pectolytic enzymes: ______________ Extraction and clarification of fruit juice including Random hydrolysis of (I -4)-c-D-grapes for wine production; Polygalacturonase EC 3.2.1.15 GH28 galactosiduronjc linicages in pectate extraction of plant material (SEQ ID NOs: 26-61) and other galacturonans. in brewing industry; also used for rettmg of plant material.

De-esterification of pectin into As for GH28; also used in Pectin methylesterase EC 3.1.1.11 CE8 pectate and methanol. Pre-requisite combination with GH28 w U) (SEQ ID NOs: 62-66) to the action of polygalacturonase enzymes.

enzymes.

Lyase enzyme: I Cleaves uronic acid-containing polysaccharaide chains via a J3-Rhamnogalacturonan lyase EC4.2.2 PL4 (SEQ ID NOs:143-147) elimination mechanism to produce 2 a terminal hexenuronjc acid ____ moiety'. _______ aEnzyme nomenclature and classification commission, http://www.chem.qmul.ac.ukJiuym/ enzyme database, http://www.cazy.org/; GH: glycoside hydrolase; CE: carbohydrate esterase; PL: polysaccharide lyase I got this information from the Cazy website. Please let me know if there are any problems with this.

2PJeasc can you provide some uses? Table 3 -codon usage in plant cell wall degrading enzyme messages #Codon (ammo acid) PCWDES C.maculatus C.tremulae G.virlduJa Ldecemlineata S.oryzae T.castaneum D.melanogaster Nosema S.cerevlsiae Wolbachia GCA (A) 0,287 Qf Q Q QIIli 0,27 0,29 GCC(A) 0,236 0,201 0,219 0,204 0,211 0,215 0,2 0,234 0,22 0,22 0,146 GCG(A) 0,161 0,132 0,116 0,128 0,127 0,145 0,197 0,187 0,15 0,11 0,11 GCT(A) 0,321 0,312 0,306 0,314 0,318 0,274 0,265 Q3 QI1 0,357 TGC(C) 0,414 0,392 0,38 0,362 0,376 0,351 0,361 0,483 0,26 0,37 QQ TGT (C) Q,5p. QQ QZ4 Qq Q.6i ___________ QZ4 Q51 0,497 GAC(D) 0,433 0,381 0,346 0,347 0,366 0,373 0,407 0,416 0,26 0,35 0,273 GAT(D) 0,567 0,619 0,654 0,653 0,634 0,627 0,593 0,584 0,74 0,65 0,727 GAA(E) 0,65 0,66 0,68 0,682 0,678 0,689 0,698 0,611 0,72 0,7 0,668 GAG (E) 0,35 0,34 0,32 0,3 18 0,322 0,3 11 0,302 0,389 0,28 0,3 0,332 TrC(F) 0,477 0,358 0,386 0,41 0,387 0,315 0,251 0,327 0,26 0,41 0,316 lTr(F) 0,523 0,642 0,614 0,59 0,613 0,685 0,749 0,673 0,74 0,59 0,684 GGA (G) Q, Q Q!Z 2Q Q £1 0,22 GGC(G) 0,198 0,203 0,185 0,185 0,184 0,197 0,213 0,273 0,12 0,19 0,201 GGG (G) 0,178 0,167 0,186 0,169 0,177 0,17 0,192 0,214 0,22 0,12 0,145 GGT(G) 0,248 0,292 0,274 0,282 0,28 0,292 0,29 0,227 0,17 PAZ 0,324 CAC(H) 0,44 0,387 0,372 0,366 0,378 0,377 0,43 0,443 0,31 0,36 0,366 CATCH) 0,56 0,613 0,628 0,634 0,622 0,623 0,57 0,557 0,69 0,64 0,634 ATA(I) 0,312 0,358 0,339 0,355 0,335 0,366 0,314 0,337 0,33 0,27 0,377 ATCO) 0,315 0,244 0,24 0,24 0,241 0,207 0,171 0,222 0,16 0,26 0,213 ATF(I) 0,373 0,398 0,421 0,405 0,424 0,427 0,515 0,441 0,51 0,46 0,41 AM (K) 0,612 0,665 0,674 0,676 0,683 0,712 0,963 0,819 0,69 0,58 0,667 MG (K) 0,388 0,335 0,326 0,3 24 0,3 17 0,288 0,037 0,181 0,31 0,42 0,333 -... --..

CTA (L) 0,134 0,13 0,12 0,127 0,115 0,127 0,107 0,104 0,11 0,14 0,136 CTC(L) 0,14 0,107 0,117 0,12 0,121 0,093 0,086 0,124 0,06 0,06 0,095 CTG (L) 0,202 0,15 0,146 0,143 0,147 0,125 0,094 0,151 0,07 0,11 0,11 CTT(L) 0,158 0,187 0,184 0,179 0,19 0,182 0,165 0,175 0,21 0,13 0,205 Table 3 -codon usage in plant cell wall degrading enzyme messages #Codon (amino acid) PCWDES C.macuIat C.tre,nuiae G.viridula Ldecemllneata S.oryzae T.castaneum D.melanogaster Nosema S.cerevlsiae WoJbc TTA(L) 0,161 0,207 0,195 0,188 0,196 0,27 Q? Q21 QI22 TTG (L) QJJ Q23 QJJ. Q,g QQ 0,222 _____________ 0,17 Q22 0,195 ATG(M) 1 1 1 1 1 1 1 1 1 1 1 AAC(N) 0,435 0,398 0,362 0,356 0,382 0,367 0,334 0,373 0,27 0,41 0,338 AAT(N) 0,565 0,602 0,638 0,644 0,618 0,633 0,666 0,627 0,73 0,59 0,662 CCA (P) 0,398 0,389 0,405 0,401 0,39 0,379 0,345 0,354 0,33 0,42 0,398 CCC(P) 0,188 0,167 0,186 0,167 0,178 0,171 0,191 0,213 0,1.3 0,15 0,152 CCG (P) 0,16 0,13 0,117 0,131 0,135 0,15 0,207 0,202 0,16 0,12 0,104 CCT(P) 0,255 0,314 0,291 0,301 0,297 0,3 0,257 0,231 0,38 0,31 0,346 CAA (Q) 0,629 0,591 0,616 0,6 19 0,615 0,62 0,702 0,598 0,69 0,69 0,644 CAG(Q) 0,371 0,409 0,384 0,381 0,385 0,38 0,298 0,402 0,31 0,31 0,356 AGA (R) 0,252 0,342 0,372 0,359 0,346 0,339 0,261 0,225 0,48 0,48 0,406 AGG (R) 0,219 0,208 0,211 0,19 0,198 0,186 0,144 0,16 0,24 0,21 0,211 CGA(R) 0,167 0,146 0,151 0,175 0,165 0,158 0,192 0,182 0,1 0,07 0,104 CGC (R) 0,087 0,087 0,071 0,073 0,073 0,084 0,117 0,15 1 0,04 0,06 0,093 CGG(R) 0,134 0,084 0,086 0,081 0,09 0,093 0,115 0,14 0,06 0,04 0,066 CGT(R) 0,141 0,134 0,109 0,122 0,128 0,14 0,17 0,142 0,08 0,14 0,12 AGC(S) 0,147 0,14 0,12 0,108 0,114 0,123 0,119 0,169 0,07 0,11 0,168 AGI(S) 0,17 0,185 0,177 0,181 0,178 0,193 0,2 0,171 0,25 0,16 0,195 TCA (5) Q25 Q Q24, 0,21 0,21 0,21. 0,221 TCC(S) 0,15 0,139 0,146 0,133 0,149 0,142 0,125 0,156 0,08 0,16 0,121 TCG (S) 0,111 0,094 0,09 0,107 0,102 0,106 0,14 0,143 0,09 0,1 0,06 TCT(S) 0,172 0,214 0,214 0,211 0,214 Q.22Q 0,191 0,174 _________________________ ACA (1) Q Q Q Q Q Q 0,32 0,3 0,322 ACC(T) 0,222 0,2 0,212 0,184 0,198 0,192 0,17 0,199 0,14 0,22 0,204 ACG (1) 0,186 0,142 0,124 0,133 0,134 0,149 0,178 0,179 0,19 0,14 0,115 ACT(T) 0,287 0,291 0,294 0,302 0,308 0,298 0,287 0,275 Q, Q Table 3 -codon usage in plant cell wall degrading enzyme messages #Codon (amino acid) PCWDEs C.maculatus C.tremulae G.viridula Ldecemlineata S.oryzae T.castaneum D.melanogaster Nosenl3 S.cerevlsiae Wo1bachi GTA (V) 0,236 0,273 0,255 0,269 0,254 0,28 0,245 0,225 0,33 0,21 0,317 GTC(V) 0,215 0,187 0,18 0,183 0,186 0,178 0,172 0,196 0,14 0,21 0,131 GTG (V 0,23 0,213 0,224 0,222 0,205 0,186 0,205 0,257 0,18 0,19 0,201 GTT(V) 0,318 0,327 0,341 0,326 0,355 0,356 0,378 0,322 0,35 0,39 0,351 TGG(W) 1 1 1 1 1 1 1 1 1 1 1 TAC(Y) 0,464 0,378 0,341 0,34 0,356 0,342 0,34.8 0,351 0,38 0,44 0,335 TAT(Y) 0,536 0,622 0,659 0,654 0,644 0,652 0,652 0,649 0,62 0,56 0,665 TM (*) 0,385 0,355 0,333 0,37 Q4Z. QJA4 QI41 Q,4Z TAG(*) 0,193 0,243 0,221 0,226 0,216 0,226 0,179 0,212 0,1 0,23 0,241 TGA(*) QL 0,373 Q44 Q41. Q4 0,295 0,277 0,33 0,2 0,3 0,293 U) Table 4 -codon usage in plant cell wall degrading enzyme messages #Codonmko add) _____ f U TGT(C) 12 12Q 12 12 12 112 7 GAC(D) 7 7 4 5 4 4 3 4 4 10 2 GAT(D) 12 12 12 2 1 GAA(E) GAG(E) 6 6 4 4 4 3 2 5 7 6 3 Trc(F) 9 9 12 12 12 7 4 6 4 9 7 (F) 4Q Ui GGA(G) 11. Z 2 2 GGC(G) 2 2 1 1 1 1 1 4 0 1 1 GGG(G) 2 2 1 1 1 1 0 2 2 0 0 GGT(G) 3 3 3 3 3 3 2 2 11 CAC(I-$) 6 6 5 5 5 4 3 6 1 3 5 CAT(H) 12 12 22 1 1 12 1 12 12 ATAQ) 6 6 12 13 12 14 5 10 9 5 15 ATC(I) 6 6 4 4 4 3 1 3 2 5 4 AU(J) 11 Li 1 1 1 1 AAA(K) 41 41 4Z 41 AAG (K) 9 9 6 6 6 6 0 3 10 15 11 CTA(L) 1 1 1 1 1 1 0 1 1 1 2 CTC(L) 1 1 1 1 1 0 0 1 0 0 1 CTG(L) 2 2 2 1 0 2 0 1 1 CTT(L) 1 1 3 3 3 3 2 3 5 1 5 IabIe 4 -codon usage in plant cell wall degrading enzyme messages #Codon(amuio add) TTG(L) 5 4 2 2 4 CCC(P) 1 1 1 1 1 1 0 2 0 1 0 CCG(P) 1 1 0 0 0 0 1 2 0 0 0 CCT(P) 3 3 3 3 3 3 1 2 6 4 5 CAA(Q) 2Z 21 LI 22 CAG(Q) 8g 7 7 7 6 2 7 2 2 6 AGA (R) 2 5 1Q 12 2 2 11 21 2 AGG (R) 3 3 2 2 2 2 0 1 2 4 2 CGA(R) 2 2 1 1 1 1 1 2 0 0 0 CGC(R) 0 0 0 0 0 0 0 1 0 0 0 CGG(R) 1 1 0 0 0 0 0 1 0 0 0 CGT(R) 1 1 0 0 1 1 1 1 0 2 0

SEQUENCE LISTING

<110> University of Exeter Pauchet, Yannick ffrench-Constant, Richard <120> Enzymes <130> RT/P1669GB02 <150> GB1006001.0 <151> 2010-04-09 <150> U561/322667 <151> 2010-04-09 <150> GB1021379.1 <151> 2010-12-16 <160> 167 <170> Patentln version 3.5 <210> 1 <211> 243 <212> PRT <213> Chrysomela tremulae <400> 1 Met Lys Leu Ser Val Val lie Leu Thr Thr Tyr Leu Ser Leu Gly Tyr 1 5 10 15 Ser Ala Ser Glu Pro Ser Pro Thr lie lie Pro Arg Gin Gly Gly Leu 25 30 Ser Gly Thr Gly lie Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser 40 45 Cys Ser Trp Ala Asp His Val Thr Tyr Thr Lys Asn Ala Thr Pro Val 55 60 Thr Ser Cys Ser Val Asp Gly Val Thr Glu lie Asp Ala Gly lie Gin 70 75 80 Ser Gly Cys Ala Glu Asp Gly Ser Gly Ser Ser Tyr Thr Cys Ser Asn 90 95 Gin Gin Pro Phe lie Val Asn Ser Thr Leu Ala Tyr Gly Phe Ala Ala 105 110 Ala Ser Phe lie Gly Gly Glu Asp Tyr Gly Trp Cys Cys Ser Cys Met 120 125 Leu Leu Ser Phe Gin Gly Gin Leu Ala Gly Lys Gin Met Leu Val Gin 135 140 Val Thr Asn Thr Giy Ser Pro Leu Ser Thr Asn Gin Phe Asp lie Glu 150 155 160 lie Pro Giy Giy Giy Val Giy Leu Tyr Pro Tyr Giy Cys Ala Thr Gin 170 175 Trp Gly Ala Asp Pro Glu Ser Gly Trp Gly Glu Arg Tyr Gly Gly Val 185 190 Ser Ser Ala Giu Giu Cys Ser Gin Leu Pro Giu Ser Leu Gin Ala Giy 200 205 Cys Leu Trp Arg Phe Asp Phe Met Glu Gly Val Ser Asn Pro Asn Val 210 215 220 Thr Phe Tyr Gin Val Glu Cys Pro Ser Glu Leu Val Ala lie Thr Gly 225 230 235 240 Cys Asp Tyr <210> 2 <211> 242 <212> PRT <213> Chrysomela tremuiae <400> 2 Met Asn Phe Leu Ala lie Gly Ser Leu Val lie Leu Ala Leu Ala Asn 1 5 10 15 Cys Lys Ala Asp His Gly Ser Glu Glu lie lie Leu Lys Gin Gly Gly 25 30 Leu Ser Gly Thr Gly Thr Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro 40 45 Thr Cys Ala Trp Pro Gly His Val Asp Tyr Lys Ser Pro Val Arq Ser 55 60 Cys Glu Ala Asn Gly Val Thr Thr Ile Asp Pro Glu Val Gln Ser Gly 70 75 80 Cys Glu Ser Asp Gly Arg Ser Tyr Ile Cys Thr Asp Gln Gly Gly Phe 90 95 Ala Ile Asn Ser Thr Leu Ala Tyr Gly Phe Ala Ala Ala Arg Phe Ile 105 110 Asn Thr Glu Arg Asn Met Cys Cys Ala Cys Val Leu Phe Thr Phe Gln 120 125 Ser Asp Glu Leu Lys Asp Lys Lys Met Leu Val Gln Val Thr Asn Thr 135 140 Gly Asn Ala Pro Glu Ser Gly Thr Asn Leu Phe Asp Ile Ala Met Pro 150 155 160 Gly Ser Gly Val Gly Tyr Tyr Thr Gln Gly Cys Thr Ser Gln Trp His 170 175 Ser Asp Val Ser Asn Trp Gly Asp Gln Tyr Gly Gly Val Arg Glu Glu 185 190 Ala Asp Cys Tyr Asn Leu Pro Lys Trp Leu Trp Asp Gly Cys Lys Phe 200 205 Arg Phe Glu Trp Met Lys Gly Val Ser Asn Pro Pro Val Ser Phe Val 210 215 220 Glu Val Glu Cys Pro Lys Lys Leu Leu Ser Ile Ser Gly Cys Asn Pro 225 230 235 240 Val Ser <210> 3 <211> 241 <212> PRT <213> Gastrophysa viridula <400> 3 Met Gln Phe Leu Ala Val Gly Ser Leu Val Ile Val Ala Leu Ala Thr 1 5 10 15 Cys Ser Ala Glu Asn Ser Pro Glu Ile Ile Gln Ile Glu Gly Gly Leu 25 30 Ser Gly Thr Gly Pro Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro Thr 40 45 Cys Ser Trp Pro Gly Asn Val Glu Tyr Lys Thr Pro Val Lys Ser Cys 55 60 Ala Ala Asp Gly Val Thr Ala Asn Asp Pro Glu Ile Gln Ser Gly Cys 70 75 80 Glu Lys Asp Gly His Ser Tyr Ile Cys Thr Asn Gln Ala Gly Phe Val 90 95 Val Asn Ser Thr Leu Ala Tyr Gly Phe Ala Ala Ala Arg Phe Ile Gly 105 110 Thr Lys Arg Asn Met Cys Cys Ser Cys Val Leu Phe Ser Phe Gln Gln 120 125 Glu Gln Leu Lys Gly Lys Lys Met Leu Val Gln Val Thr Asn Thr Gly 135 140 Asn Ala Pro Glu Thr Gly Thr Asn Leu Phe Asp Ile Ala Met Pro Gly 150 155 160 Ser Gly Val Gly Tyr Tyr Thr Gln Gly Cys Thr Ser Gln Trp Asn Ser 170 175 Asp Val Ser Asn Trp Gly Asp Gln Tyr Gly Gly Val Asn Ser Glu Ala 185 190 Asp Cys Tyr Lys Leu Pro Gln Ala Leu Trp Glu Gly Cys Arg Phe Arg 200 205 Phe Thr Trp Met Gln Gly Val Ser Asn Pro Asp Val Ser Phe Tyr Glu 210 215 220 Val Gln Cys Pro Lys Glu Leu Leu Asp Ile Ser Gly Cys His Pro Val 225 230 235 240 Gly <210> 4 <211> 240 <212> PRT <213> Leptinotarsa decemlineata <400> 4 Met Lys Gly Phe VaJ Val Leu Leu lie Thr Val Ma Phe Phe Asp Ma 1 5 10 15 Ser Thr Gly Glu Pro Ser Pro Glu lie lie Pro Val Glu Gly Gly Leu 25 30 Ser Gly Asp Gly lie Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser 40 45 Cys Gly Trp Ala Asp Leu Val Asn Asn Thr Arg Phe Lys Thr Pro Val 55 60 His Thr Cys Ala lie Asp Giy lie Giu lie lie Asp Pro Gin Gin Gin 70 75 80 Ser Gly Cys Ala Asp Vai Giy Ala Ala Tyr Thr Cys Ser Asn Gin Gin 90 95 Pro Phe Val Val Asn Ser Thr Leu Ala Tyr Gly Phe Ser Ala Val Ser 105 110 Phe Thr Giy Gly Glu Asp Tyr His Met Cys Cys Ser Cys Met Leu Leu 120 125 Asn Phe Gin Gly Gin Leu Ser Gly Lys Lys Met Leu Vai Gin lie Thr 135 140 Asn Thr Giy Ser Pro Leu Ala Vai Asn Gin Phe Asp lie Giu Leu Pro 150 155 160 Giy Gly Giy Val Giy lie Tyr Pro His Giy Cys Met Lys Gin Trp Asn 170 175 Ala Ser Glu Thr Gly Trp Gly Asp Thr Phe Gly Gly Val His Ser Asp 185 190 Ala Glu Cys Ser Glu Leu Pro Ala Val Leu Gln Pro Gly Cys His Phe 200 205 Arg Phe Gln Phe Met Glu Gly Val Ser Asn Pro Pro Val Thr Phe Gln 210 215 220 Gln Val Gln Cys Pro Arg Glu Leu Val Ala Ile Thr Gly Cys Glu Tyr 225 230 235 240 <210> 5 <211> 242 <212> PRT <213> Leptinotarsa decemlineata <400> 5 Met Lys Leu Leu Val lie Ser Leu Val Leu Ala Ala Gly Tyr Ala Ser 1 5 10 15 Pro His Asp Arg Ser Pro Asp Ile Ile Pro Ile Asp Gly Gly Val Lys 25 30 Gly Asp Gly Val Thr Thr Arg Tyr Trp Asp Cys Cys Ala Pro Ser Cys 40 45 Ala Trp Asp Glu Ile Val His Thr Lys Asn Gly Ile Pro Ile Gln Thr 55 60 Cys Gln Lys Asp Gly Ile Thr Pro Ser Arg Lys Glu Asp Asn Ala Gln 70 75 80 Ser Gly Cys Val Glu Gly Gly Gln Ala Tyr Thr Cys Thr Asn Gln Ser 90 95 Pro Tyr Leu Val Asn Glu Thr Leu Ala Phe Gly Phe Ser Ala Ser Ser 105 110 Phe Asn Gly Gly Ile Asp Thr Ala Gln Cys Cys Met Cys Val Leu Leu 120 125 Ser Phe Lys Asp Gln Leu Ala Gly Lys Gln Met Leu Val Gln Leu Thr 135 140 Asn Thr Gly Ser Asp Leu Gly Gln Asn His Phe Asp Ile Ala Ile Pro 150 155 160 Gly Gly Gly Val Gly lie Phe Thr Leu Gly Cys Ser Thr Gin Trp Gly 170 175 Val Pro Glu Asn Gly Trp Gly Glu Arg Tyr Gly Gly Val Thr Ser lie 185 190 Glu Glu Cys Asp Glu Leu Pro Asp Val Leu Gin Giu Giy Cys Arg Phe 200 205 Arg Phe Thr Phe Met Giu Giy Vai Ser Asn Pro Ala Vai Ser Phe Tyr 210 215 220 Gin Vai Lys Cys Pro Aia Glu Leu lie Asp lie Ser Lys Cys Gly Asp 225 230 235 240 Arg Asp <210> 6 <211> 243 <212> PRT <213> Leptinotarsa decemlineata <400> 6 Met Arg His Val lie Vai Leu Ala Leu lie lie Phe Giy Tyr Val Ser 1 5 10 15 Thr Cys Thr Ser Glu Pro Ser Pro Glu lie lie Pro lie Pro Gly Gly 25 30 Lys Gin Giy Thr Giy Vai Thr Thr Arg Phe Trp Asp Cys Cys Lys Pro 40 45 Ser Cys Ser Trp Arg Giy Asn Leu Arg Asn Thr Ser Aia Thr Pro Vai 55 60 Thr Ser Cys Gly lie Asp Giy Asp Thr Aia Vai Asp Pro Asp Leu Met 70 75 80 Ser Ala Cys Asp Leu Gin Asn Lys Giy Pro Ala Tyr Met Cys Thr Asn 90 95 Gin Gin Pro Phe Vai Vai Asn Ser Thr Leu Ala Tyr Giy Tyr Vai Ala 105 110 Ala Ser Phe Thr Gly Ser Thr Asp Tyr Gin Leu Cys Cys Gly Cys Val 120 125 Leu Leu Ser Phe Gin Gly Gin Leu Ala Gly Lys His Leu Leu Ala Gin 135 140 Val Thr Asp Ala Gly Ser Asp Leu Val Val Asn Gin Phe Asp lie Ala 150 155 160 lie Pro Gly Gly Gly Val Gly lie Gly Asn Gly Cys Thr Glu Gin Trp 170 175 Asn Ala Pro Pro Asp Gly Trp Gly Ala Arq Tyr Gly Gly lie Ser Thr 185 190 Glu Glu Glu Cys Asp Glu Leu Pro lie Gin Leu Gin Pro Gly Cys His 200 205 Phe Arg Phe Gin Phe Met Glu Gly Val Pro Asn Pro Asp Val Thr Phe 210 215 220 lie Gin Val Glu Cys Pro Arg Glu Leu Val Asp Thr Thr Gly Cys Glu 225 230 235 240 Leu Arg Val <210> 7 <211> 237 <212> PRT <213> Leptinotarsa decemlineata <400> 7 Met Val Thr Ala Leu Ala Phe lie Leu Phe Ala Cys Val Ala Ser Ser 1 5 10 15 Thr Gly Glu Asp Ser Pro Asp lie lie Pro Val Pro Gly Gly Leu Ser 25 30 Gly Thr Gly lie Thr Thr Arq Tyr Trp Asp Cys Cys Lys Pro Ser Cys 40 45 Ser Trp Arq Glu Asn Leu Arq Asn Thr Ser Ala Asp Pro Val Thr Ser 55 60 Cys Ala Ile Asp Gly Asn Thr Val Ile Asp Pro Glu Ala Met Ser Asn 70 75 80 Cys Gly Gly Gly Pro Ser Tyr Met Cys Ser Asn Gln Gln Pro Phe Val 90 95 Val Asn Ser Thr Leu Ala Tyr Gly Phe Ala Gly Ala Ser Phe Thr Gly 105 110 Lys Ala Asp Tyr Gln Leu Cys Cys Gly Cys Val Leu Leu Ser Phe Thr 120 125 Gly Ala Leu Ser His Lys Lys Met Val Val Gln Val Thr Asn Thr Gly 135 140 Ala Asp Ala Val Ala Asn Gln Phe Asp Leu Ala Leu Pro Gly Gly Gly 150 155 160 Val Gly Ile Asn Asn Gly Cys Ile Ser Gln Trp Asn Ala Pro Pro Asp 170 175 Gly Trp Gly Lys Arg Tyr Gly Gly Val Asp Thr Glu Ala Glu Cys Asp 185 190 Gln Leu Pro Arg Gln Leu Gln Asp Gly Cys Arq Phe Arg Phe Glu Phe 200 205 Met Glu Ser Val Ser Asn Pro Ser Val Asn Phe Val Gln Val Glu Cys 210 215 220 Pro Lys Glu Ile Val Asp Arg Ser His Cys Asn Leu Lys 225 230 235 <210> 8 <211> 237 <212> PRT <213> Leptinotarsa decemlineata <400> 8 Met Glu Thr Ala Leu Ala Leu Ile Leu Leu Ala Cys Val Ala Ser Ser 1 5 10 15 Thr Gly Glu Tyr Ser Pro Asp lie lie Pro Val Pro Giy Gly Leu Ser 25 30 Gly Thr Gly lie Thr Thr Gly Tyr Trp Asp Cys Cys Lys Pro Ser Cys 40 45 Ser Trp Arg Gly Asn Leu Arg Asn Thr Ser Ala Asp Pro Val Thr Ser 55 60 Cys Ala lie Asp Gly Asp Thr Val lie Asp Pro Glu Ala Met Ser Asn 70 75 80 Cys Gly Gly Giy Pro Ser Tyr Met Cys Thr Asn Gin Gin Pro Phe Vai 90 95 Vai Asn Ser Thr Leu Ala Tyr Gly Phe Thr Ala Ala Ser Phe Thr Gly 105 110 Lys Ala Asp Tyr Lys Leu Cys Cys Gly Cys Val Leu Leu Ser Phe lie 120 125 Gly Ala Leu Ser His Lys Lys Met Leu Vai Gin lie Thr Asn Thr Giy 135 140 Ala Asp Leu Vai Val Asn Gin Phe Asp Leu Ala Phe Pro Gly Ala Giy 150 155 160 Vai Gly lie Asn Asn Gly Cys lie Ser Gin Trp Asn Aia Pro Pro Asp 170 175 Gly Trp Gly Lys Arg Tyr Gly Gly Val Asp Thr Glu Ala Glu Cys Asp 185 190 Gln Leu Pro Trp Gln Leu Gln Asp Gly Cys Arg Phe Arg Phe Glu Phe 200 205 Met Glu Ser Val Pro Val Pro Asn Val Thr Phe Val Gln Val Glu Cys 210 215 220 Pro Lys Giu Leu Val Asp lie Ser His Cys Asn Leu Lys 225 230 235 <210> 9 <211> 236 <212> PRT <213> Leptinotarsa decemlineata <400> 9 Met Lys lie Ala Leu Leu Ala Leu Phe Vai Thr Tyr Ala Vai Ala Leu 1 5 10 15 Pro Ser Asp Asn Glu Asp lie lie Pro Val Pro Gly Gly Arg Ser Giy 25 30 Trp Gly Thr Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser Cys Ala 40 45 Trp Vai Glu Asn lie Lys Thr Arg Asp Met His Ala Vai Asn Thr Cys 55 60 Asp Ser Lys Gly Lys Asn Val Leu Lys Pro Ser Val Lys Ser Gly Cys 70 75 80 Asp Giy Giy Thr Ala Tyr Met Cys Asn Asn Gin Gin Pro Phe Vai Vai 90 95 Asn Lys Thr Leu Ala Tyr Giy Phe Ala Ala Ala Ser Phe Thr Giy Giy 105 110 Vai Asp Thr Asn Leu Cys Cys Ala Cys Phe Leu Leu Thr Phe Gin Giy 120 125 Gin Leu Ser Gly Lys Gin Leu Leu Val Gin Asn Thr Asn Ser Gly Gly 135 140 Asp Leu Giy Ala Asn Gin Phe Asp lie Ala Thr Pro Giy Giy Gly Val 150 155 160 Giy lie Phe Thr Ser Giy Cys His Asp Gin Trp Asn Ala Pro Trp Ser 170 175 Giy Trp Giy Asp Gin Tyr Giy Giy Vai His Ser Ala Giu Giu Cys Asn 185 190 Thr Leu Pro Gin Giu Leu Gin Ser Giy Cys Arq Phe Arg Phe Giu Phe 200 205 Met Giu Asn Vai Ser Asn Pro Gin Vai Gin Phe Gin Gin Vai Vai Cys 2i0 2i5 220 Pro Lys Lys Leu Vai Giu lie Thr Giy Cys Asn Leu 225 230 235 <2i0> iO <2ii> 243 <2i2> PRT <2i3> Leptinotarsa decemilneata <400> iO Met lie Vai Asn lie lie Ser Phe Leu Phe Vai lie Thr Ser Cys Vai i 5 10 15 Thr Aia Giy Thr lie Asp Tyr Tyr Pro Asn Vai Giu Vai lie Giu Giy 25 30 Giy lie Ser Giy Asp Aia lie Thr Thr Arg Tyr Trp Asp Cys Cys Lys 40 45 Pro Ser Cys Giy Trp Arg Giy Asn Leu Asn Asp His Thr Aia Asn Pro 55 60 Vai Thr Ser Cys Aia Aia Asp Giy Vai Thr Vai Leu Asp Pro Giu lie 70 75 80 Met Ala Ala Cys Asp Pro Gly Lys Asn Gly Thr Ser Tyr Met Cys Asn 90 95 Asn Gin Gin Asn Tyr Ala Val Asn Asp Thr Phe Ala Tyr Gly Tyr Val 105 110 Ala Ala Ser Phe Thr Gly Gly Val Asp Tyr Ser Tyr Cys Cys Ala Cys 120 125 Val Leu Leu Ser Phe Lys Ser Gly Leu Glu Gly Lys Tyr Met Leu Ala 135 140 Gin lie Thr Asn Ala Gly Ser Asp Leu His Ala Asn Gin Phe Asp lie 150 155 160 Ala lie Pro Gly Gly Gly Val Gly Leu His Asn Gly Cys Asp Leu Gin 170 175 Trp His Ser Gly Pro Asp Gly Trp Gly Asp Arq Val Met Gly lie Asp 185 190 Thr Arg Glu Glu Cys Asp Thr Leu Leu Pro Glu Pro Leu Arg Ser Gly 200 205 Cys Tyr Phe Arg Phe Asp Phe Met Glu Gly lie Pro Asn Pro Asp Val 210 215 220 Ser Phe Lys Gin Vai Lys Cys Pro Lys Giu Leu lie Aia Asn Thr Gly 225 230 235 240 Cys Asn Met <210> 11 <211> 235 <212> PRT <213> Sitophilus oryzae <400> 11 Met Lys Val Leu Cys Val lie Leu Ala Vai Ala Ala Phe Ala Arg Ala 1 5 10 15 Thr Asp lie His Leu Gin Lys Val Val Gly Gly lie Ser Gly Glu Ala 25 30 Asn Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser Cys Ser Trp Ala 40 45 Glu Asn Val Asn Ala Gin Ser Pro Val Ala Ser Cys Ala Val Asp Gly 55 60 Val Thr Val Leu Asp Pro Ser Ser Gly Ser Gly Cys Ala Glu Asp Gly 70 75 80 Ser Gly lie Ser Tyr Val Cys Thr Asn Gin Gin Pro Trp Ala Val Asn 90 95 Asp Thr Val Ala Phe Gly Phe Ala Ala Ala Ser Phe Ser Gly Gly Ala 105 110 Asp Asn Ser Gin Cys Cys Val Cys Leu Gin Leu Ser Phe Leu Asn Val 120 125 Leu Pro Asn Lys Lys Leu lie Vai Gin Vai Thr Asn Thr Giy Giy Asp 135 140 Leu Giu Ser Asn His Phe Asp lie Gin Leu Pro Giy Giy Giy Vai Giy i45 i50 i55 i60 Tyr Phe Thr His Giy Cys Vai Ser Gin Trp Asn Cys Pro Aia Asn Giy i65 170 i75 Trp Giy Aia Gin Tyr Giy Giy lie Thr Ser Giu Ser Giu Cys Asn Giu i80 i85 190 Leu Pro Giu Vai Leu Gin Pro Giy Cys His Phe Arg Phe Asn Trp Phe i95 200 205 Giu Asn Aia Asp Asn Pro Lys Vai Thr Phe Giu Gin Vai Vai Cys Pro 2i0 2i5 220 Ser Giu Leu Thr Ser lie Thr Giy Cys Vai Vai 225 230 235 <210> 12 <211> 232 <212> PRT <213> Sitophiius oryzae <400> 12 Met Lys Leu Leu Cys Leu Phe Leu Aia Aia Aia Thr Vai Vai Gin Aia 1 5 10 15 Arg Asp lie His Leu Gin Gin Vai Vai Giy Giy Vai Ser Giy Ser Giy 25 30 Thr Thr Thr Arg Tyr Trp Asp Cys Cys Lys Pro Ser Cys Aia Trp Lys 40 45 Giu Asn Vai Giu Thr Aia Ser Pro Vai Aia Ser Cys Aia Aia Asp Giy 55 60 Vai Thr Thr Vai Asp Pro Ser Thr Arg Ser Giy Cys Asp Ser Asp Giy 70 75 80 Ser Ser Tyr Thr Cys Asn Asn Gin Gin Pro Trp Vai Vai Asn Ser Thr 90 95 Phe Ala Phe Gly Phe Ala Ala Ala Ser Phe Thr Gly Gly Ala Asp Asn 105 110 Ser Gln Cys Cys Ile Cys Val Gln Leu Ser Phe Leu Ser Thr Ile Thr 120 125 Glu Lys Thr Leu Val \7a1 Gln Val Thr Asn Thr Oly Ala Asp Leu Gly 135 140 Ser Asn His Phe Asp Ile Gln Leu Pro Gly Gly Gly Val Gly Tyr Phe 150 155 160 Thr Thr Gly Cys Ser Ser Gln Trp Asn Cys Pro Ala Asn Gly Trp Gly 170 175 Ser Gln Tyr Gly Gly Ile Thr Ser Asp Ser Glu Cys Ser Glu Leu Pro 185 190 Glu Val Leu Gln Pro Gly Cys His Phe Arg Phe Asn Trp Phe Gln Asn 200 205 Ala Asp Asn Pro Asn Val Asn Phe Gln Gln Val Val Cys Pro Ser Glu 210 215 220 Leu Thr Ser Ile Thr Gly Cys Ala 225 230 <210> 13 <211> 247 <212> PRT <213> Sitophilus oryzae <400> 13 Met Lys Val Leu Ile Val Leu Thr Ile Tyr Leu Gly Ala Val Phe Cys 1 5 10 15 Gly Ser Val Leu Pro Glu Arg Lys Pro Glu Ile Lys Lys Val Val Gly 25 30 Gly Phe Ser Gly Ser Cys Thr Thr Thr Arq Tyr Trp Asp Cys Cys Lys 40 45 Pro Thr Cys Ala Trp Lys Glu Asn Ile Asp Thr Asp Val Gly Pro Val 55 60 Lys Ala Cys Asn Ile Asp Gly Val Asn Ile Ser Asp Val Glu Ala Gln 70 75 80 Ser Gly Cys Gly Gly Gly Ser Ser Tyr Met Cys Asn Asn Gln Gln Ser 90 95 Ile Ile Val Asn Ser Thr Phe Ala Tyr Gly Phe Ala Ala Ala Val Phe 105 110 Ile Ser Pro Pro Glu Asn Leu Cys Cys Ala Cys Phe Leu Val Asn Phe 120 125 Gln Lys Gly Glu Trp Gly Asp Cys Ala Gly Lys Gln Met Ile Val Gln 135 140 Leu Thr Asn Thr Gly Gly Ser Ser Ser Val Asn Gly Thr Glu Asn Asn 150 155 160 Ile Glu Ile Ala Met Pro Gly Gly Gly Val Gly Tyr Phe Thr Glu Gly 170 175 Cys Lys Thr Gln Trp Asn Ala Pro Asp Gln Gly Trp Gly Glu Gln Tyr 185 190 Gly Gly Val Thr Thr Glu Asp Gly Cys Asn Asp Leu Pro Thr Gln Leu 200 205 Gln Pro Gly Cys Lys Phe Arg Trp Glu Phe Leu Asn Gly Cys Ser Asn 210 215 220 Pro Pro Ala Thr Phe Glu Gln Val Val Cys Pro Gln Glu Ile Val Asp 225 230 235 240 Ile Ser Gly Cys Lys Thr Asp <210> 14 <211> 247 <212> PRT <213> Sitophilus oryzae <400> 14 Met Lys Val Leu lie Val Leu Thr lie Cys Leu Giy Val Val Phe Cys 1 5 10 15 Gly Ser Val lie Ser Glu Arg Lys Pro Glu lie Lys Lys Val Val Gly 25 30 Gly Phe Ser Gly Ser Cys Thr Thr Ser Arg Tyr Trp Asp Cys Cys Lys 40 45 Pro Thr Cys Ser Trp Lys Gly Asn Thr Asp Thr Asp Val Gly Pro Val 55 60 Lys Ala Cys Ser Aia Asp Giy Vai Thr Val Ser Asp Glu Asp Thr Gin 70 75 80 Ser Giy Cys Val Gly Giy Ser Ser Tyr Met Cys Asn Asn Gin Gin Ser 90 95 lie Vai lie Asn Ser Thr Leu Ala Tyr Gly Phe Ala Ala Ala Val Phe 105 110 Lys Thr Pro Pro Glu Asn Met Cys Cys Thr Cys Phe Leu Val Thr Phe 120 125 Gin Lys Gly Giu Trp Gly Asp Cys Ser Gly Lys Gin Met Val Vai Gin 135 140 Leu Thr Asn Thr Giy Gly Ser Ser Ser Val Asn Giy Thr Glu Asp Asn 150 155 160 lie Glu Ile Gly Met Pro Gly Gly Gly Val Gly Tyr Tyr Thr Glu Gly 170 175 Cys Lys Thr Gln Trp Asn Ala Pro Asp Gin Gly Trp Gly Asp Gin Tyr 185 190 Gly Gly Val Thr Thr Glu Asp Giy Cys Asn Asp Leu Pro Thr Gin Leu 200 205 Gin Ser Giy Cys Lys Phe Arq Trp Glu Phe Leu Asn Gly Cys Ser Asn 210 215 220 Pro Pro Ala Thr Phe Glu Gln Val Val Cys Pro Gln Glu lie Val Asp 225 230 235 240 lie Ser Gly Cys Lys Met Asp <210> 15 <211> 253 <212> PRT <213> Sitophilus oryzae <400> 15 Met Lys Vai Leu lie Vai Leu Thr Phe Cys lie Vai Tyr Vai Phe Cys 1 5 10 15 Gly Ser lie Leu Thr Gin Arq Gin Pro Glu lie Lys Lys Val Vai Giy 25 30 Giy Tyr Ser Giy Ser Cys Thr Thr Ser Arg Tyr Trp Asp Cys Cys Lys 40 45 Pro Thr Cys Ser Trp Lys Gly Asn Thr Asn Thr Asp Tyr Gly Pro Val 55 60 Arg Ala Cys Ser Ser Asp Gly Tyr Asn Val Asp Asp Gly Asn Thr Glu 70 75 80 Ser Gly Cys Val Gly Gly Ser Ser Tyr Met Cys Asn Asn Gin Gin Ser 90 95 Val Val lie Asn Ser Thr Leu Ala Tyr Gly Phe Ala Ala Ala Met Phe 105 110 lie Ser Pro Pro Glu Asn Met Cys Cys Thr Cys Phe Leu Leu Ser Phe 120 125 Gin Lys Gly Glu Trp Gly Asp Cys Ser Gly Lys Gin Met Val Val Gin 135 140 lie Thr Asn Thr Gly Ala Gly Ser Ser Asn Ser Ser Gly Asp Gin Asn 150 155 160 Asn lie Glu Val Ala Met Pro Gly Gly Gly Val Gly Tyr Tyr Thr Glu 170 175 Gly Cys Lys Thr Gin Trp Asn Ala Pro Asp Leu Gly Trp Gly Asp Gin 185 190 Tyr Gly Gly Val Thr Thr Glu Asp Gly Cys Tyr Glu Leu Pro Thr Gin 200 205 Leu Gin Asp Gly Cys Lys Phe Arg Trp Glu Phe Leu Asn Gly Cys Ser 210 215 220 Asn Pro Pro Ala Thr Phe Glu Gin Val Val Cys Pro Gin Glu lie Val 225 230 235 240 Asp lie Ser Gly Cys Lys Met Asp Pro Val Trp Asn Ser 245 250 <210> 16 <211> 639 <212> PRT <213> Chrysomela tremuiae <400> 16 Met Lys Ser Leu Ala lie lie Val Val Phe Gly Leu lie Ala Gin Leu 1 5 10 15 Gin Ala Asp Ser Val Tyr Val Gin Arg Phe Leu Gin Gin Tyr Asn Lys 25 30 Met His Asp Pro Ala Asn Gly Tyr Phe Ser Lys Trp Gly lie Pro Tyr 40 45 His Ala Val Glu Thr Leu lie Val Glu Ala Pro Asp His Gly His Glu 55 60 Thr Thr Ser Glu Ala Tyr Ser Tyr Trp lie Trp Leu Glu Ala Met Tyr 70 75 80 Gly Lys Val Thr Lys Asn Phe Thr Gly Phe Asn Ala Ala Trp Thr Asn 90 95 Met Glu Gin Tyr lie lie Pro Val His Ala Ser Gin Thr Gly Asn Asn 105 110 Tyr Asn Ala Trp Lys Pro Ala Thr Tyr Ser Asp Glu Leu Asp Trp Pro 120 125 Ser Giu Tyr Pro Ser Pro Met Gin Phe Asn Vai Pro Vai Giy Ser Asp 135 140 Pro lie Tyr Gin Giu Leu Vai Ser Aia Tyr Giy Ser Asp Asp Vai Tyr 150 155 160 Ser Met His Trp Leu Leu Asp Vai Asp Asn Thr Tyr Giy Phe Giy Asn i65 170 i75 Leu Pro Giy Ser Cys Giu Leu Giy Pro Gin Aia Asp Giy Pro Ser Phe i80 i85 190 lie Asn Thr Phe Gin Arg Giy Pro Gin Giu Ser Vai Trp Arg Thr lie i95 200 205 Pro Gin Thr Thr Cys Asp Ser Phe Lys Phe Giy Giy Pro Asn Giy Phe 2i0 2i5 220 Leu Asp Leu Phe Vai Giy Asp Asn Ser Tyr Thr Gin Gin Trp Lys Tyr 225 230 235 240 Ser Asn Aia Pro Asp Aia Asp Aia Arg Aia Vai Gin Giy Aia Phe Trp 245 250 255 Aia Tyr Thr Trp Aia Asn Giu Asn Giy Gin Aia Aia Asp Vai Ser Aia 260 265 270 Thr Vai Giu Lys Aia Giy Lys Leu Giy Asp Tyr Leu Arg Tyr Aia Leu 275 280 285 Phe Asp Lys Tyr Phe Lys Arg lie Giy Asn Cys Vai Giy Pro Tyr Asn 290 295 300 Cys Pro Giy Giy Gin Giy Lys Giu Ser Aia His Tyr Leu lie Ser Trp 305 310 315 320 Tyr Phe Aia Trp Giy Aia Aia Leu Trp Giy Giy Trp Ser Trp Arg lie 325 330 335 Giy Asp Giy Aia Aia His Phe Giy Tyr Gin Asn Pro Leu Aia Aia Tyr 340 345 350 Aia Leu Ser His Vai Asp Aia Met Arg Pro Lys Aia Aia Thr Giy Vai 355 360 365 Ser Asp Trp Gin Thr Ser Leu Asp Arg Gin Leu Giu Phe Tyr Gin Tyr 370 375 380 Leu Gin Ser Vai Giu Giy Aia Phe Aia Giy Giy Aia Thr Asn Thr Trp 385 390 395 400 Asn Giy Arg Tyr Giy Thr Pro Pro Asp Asn Leu Thr Thr His Thr Phe 405 4i0 4i5 His Giy Met Phe Tyr Asp Trp Giu Pro Vai Tyr His Asp Pro Pro Ser 420 425 430 Asn Arq Trp Tyr Giy Met Gin Pro Trp Ser Vai Asp Arg Leu Aia Gin 435 440 445 Tyr Tyr Tyr Vai Thr Giy Asp Aia Thr Aia Lys Thr Leu Leu Asp Lys 450 455 460 Trp Vai Ser Trp lie lie Ser Giu lie Lys Phe Giu Asn Asn Asn Tyr 465 470 475 480 Leu Vai Pro Giu Trp Leu Giu Trp Thr Giy lie Pro Pro Giu Vai His 485 490 495 Vai Thr Vai Thr Lys Arg Ser Ser Asp Vai Giy Thr Aia Ser Aia Thr 500 505 5i0 Aia Arg Thr Leu Ser Tyr Tyr Aia Aia Lys Ser Giy Asp Thr Asn Aia 5i5 520 525 Lys Aia Vai Aia Lys Gin Leu Leu Asp Leu lie Trp Asn Leu Tyr Gin 530 535 540 Thr Asp Lys Giy Vai Ser Met Asp Giu Vai Met Asp Thr Tyr Ser Arg 545 550 555 560 Phe Asn Giu Giu Vai Tyr Vai Pro Tyr Asn Trp Vai Giy His Tyr Pro 565 570 575 Ser Giy Asp Vai lie Gin His Giy Aia Thr Phe lie Giy Leu Arq Ser 580 585 590 Phe Tyr Lys Asn Asp Lys Asp Trp Pro Lys Val Glu Ala Tyr Leu Asn 595 600 605 Gly Gly Glu Ala Pro Thr Phe Lys Tyr His Arg Phe Trp Ser Gin Ser 610 615 620 Asp Val Ala Leu Ala Gin Gly Thr Tyr Gly lie Leu Phe Asn Glu 625 630 635 <210> 17 <211> 647 <212> PRT <213> Chrysomela tremuiae <400> 17 Met Asp Arg Ala Asn Met Giy lie Leu Ala Leu Thr Leu Vai Vai Ala 1 5 10 15 Leu Ala Thr Gin lie Asp Ala Gly Asn Val Tyr Arg Glu Arg Phe lie 25 30 Gin Gin Tyr Asn Lys lie His Asp Asp Ala Asn Gly Tyr Phe Ser Lys 40 45 Glu Gly Val Pro Tyr His Ala Val Glu Thr Leu Leu Val Glu Ala Pro 55 60 Asp His Gly His lie Thr Thr Ser Glu Ala Tyr Ser Tyr Tyr lie Trp 70 75 80 Leu Glu Ala Met Tyr Gly Gly lie Thr Lys Asp Phe Ala Pro Phe Asn 90 95 Lys Ala Trp Gin lie Met Glu Glu Phe lie lie Pro Val His Asp Ser 105 110 Gin Ser Thr Asn Ser Ala Tyr Asn Pro Ser His Pro Ala Thr Tyr Ala 120 125 Ala Glu Phe Asp Ser Pro Asp Gin Tyr Pro Ser Leu Val Asp Ser Ser 135 140 Val Pro Val Gly Gin Asp Pro lie Tyr Gin Glu Leu Val Asn Ala Tyr 150 155 160 Gly Thr Pro Asp lie Tyr Ala Met His Trp Leu Leu Asp Val Asp Asn 170 175 lie Tyr Gly Phe Gly Asn Thr Gin Giy Asn Cys Glu Giy Giy Pro Tyr 185 190 Thr Ser Gly Pro Ser Phe lie Asn Thr Phe Gin Arg Giy Ser Gin Glu 200 205 Ser Val Trp Lys Thr lie Pro Gin Pro Cys Cys Asp Ser Phe Lys Tyr 210 215 220 Gly Gly Pro Asn Gly Phe Leu Asp Leu Phe Thr Lys Asp Asn Ser Tyr 225 230 235 240 Ala Lys Gin Trp Lys Tyr Thr Val Ala Pro Asp Ala Asp Ala Arg Ala 245 250 255 lie Gin Ala Ala Phe Trp Ala Ala Gin Trp Ala Gin Glu Val Gly Gin 260 265 270 Phe Ser Ala lie Ser Asp Thr Leu Ser Lys Ala Ala Lys Leu Gly Asp 275 280 285 Tyr Leu Arq Tyr Ala Leu Phe Asp Lys Tyr Phe Lys Lys Val Gly Asn 290 295 300 Cys Val Gly Pro Trp Ser Cys Gin Gly Gly Tyr Ser Lys Asp Ser Ala 305 310 315 320 His Tyr Leu Leu Gly Trp Tyr Phe Ala Trp Gly Gly Ser Leu Asp Thr 325 330 335 Ser Asn Pro Trp Ala Trp Arg lie Gly Asp Gly Ala Ala His Phe Gly 340 345 350 Tyr Gin Asn Pro Leu Thr Ala Tyr Ala Leu Val Asn Glu Pro Ser Leu 355 360 365 Lys Pro Lys Gly Ala Ser Ala Val Thr Asp Trp Gin lie Ser Leu Asp 370 375 380 Arg Gin Leu Giu Phe Tyr Giu Trp Leu Gin Thr Ser Giu Giy Aia Phe 385 390 395 400 Aia Giy Giy Aia Ser Asn Ser Trp Asn Giy Arg Tyr Asp Thr Pro Pro 405 4i0 4i5 Ser Giu Leu Thr Giy Asn Thr Phe His Giy Met Phe Tyr Asp Trp Giu 420 425 430 Pro Vai Tyr His Asp Pro Pro Ser Asn Arg Trp Phe Giy Met Gin Pro 435 440 445 Trp Ser Vai Asp Arg Leu Aia Gin Leu Tyr Tyr Vai Thr Giy Asn Ser 450 455 460 Arg Aia Lys Asn Leu Leu Asp Lys Trp Vai Ser Trp Vai Leu Ser Giu 465 470 475 480 lie Thr Phe Gin Giy Asn Gin Tyr Ser lie Pro Aia Thr Leu Giu Trp 485 490 495 Ser Giy Vai Pro Pro Asn Vai His Vai Thr Vai Thr Aia His Thr Asn 500 505 5i0 Asp Vai Giy Thr Aia Ser Aia Thr Aia Arg Thr Leu Aia Phe Tyr Aia 5i5 520 525 Aia Lys Ser Giy Asp Gin Asn Aia Lys Asn Vai Aia Lys Gin Leu Leu 530 535 540 Asp Giy Met Trp Giu Leu Tyr Gin Thr Asp Lys Giy Vai Ser Asn Ser 545 550 555 560 Giu lie Aia Asp Thr Tyr Asn Gin Phe Ser His Asp Vai Tyr Vai Pro 565 570 575 Asn Giy Trp Tyr Giy Gin Tyr Pro Asn Giy Asp Vai lie Gin Aia Pro 580 585 590 Aia Thr Phe lie Giy Leu Arq Ser Trp Tyr Lys Lys Asp Pro Aia Trp 595 600 605 Pro Lys Vai Giu Aia Tyr Leu Asn Giy Giy Ser Aia Pro Giu Phe Thr 6i0 6i5 620 Phe His Arq Phe Trp Ala Gln Ala Asp Val Ala Leu Ser Gln Gly Thr 625 630 635 640 Tyr Gly Met Leu Phe Asn Glu <210> 18 <211> 642 <212> PRT <213> Gastrophysa viridula <400> 18 Met Gly Pro Leu Ala Leu Thr Leu Leu Val Ala Leu Val Ala His Thr 1 5 10 15 Asn Ala Asn Asn Val Tyr Thr Thr Arg Phe Leu Thr Gln Tyr Lys Lys 25 30 Ile His Asn Ala Ala Ser Gly Tyr Phe Ser Lys Glu Gly Val Pro Tyr 40 45 His Ala Ala Glu Thr Leu Leu Val Glu Ala Pro Asp Tyr Gly His Glu 55 60 Thr Thr Ser Glu Thr Leu Ser Tyr Tyr Ile Trp Met Glu Ala Met Tyr 70 75 80 Gly Ala Ile Met Asn Asp Phe Gly Pro Phe Asn Lys Ala Trp Glu Val 90 95 Met Glu Glu Phe Met Ile Pro Val His Asp Ser Gln Pro Thr Asn Ser 105 110 Gly Tyr Asn Pro Ser Arg Pro Ala Thr Tyr Ala Ala Glu His Asp Thr 120 125 Pro Asn Glu Tyr Pro Ser Leu Val Asp Ser Ser Val Ser Val Gly Gln 135 140 Asp Pro Ile Tyr Gln Glu Leu Val Ser Ala Tyr Gly Thr Ser Asp Ile 150 155 160 Tyr Ala Met His Trp Leu Ser Asp Val Asp Asn Ile Tyr Gly Phe Gly 170 175 Asn Thr Gin Gly Asn Cys Glu Ala Giy Pro Ser Ala Ser Giy Pro Ser 185 190 Phe lie Asn Thr Phe Gin Arg Gly Ser Gin Glu Ser Val Trp Lys Thr 200 205 lie Pro Gin Pro Cys Cys Asp Ser Phe Lys Tyr Giy Giy Gin Asn Giy 210 215 220 Phe Leu Asp Leu Phe Thr Lys Asp Asn Ser Tyr Ala Arg Gin Trp Lys 225 230 235 240 Tyr Thr Ala Ala Pro Asp Ala Asp Ala Arq Ala lie Gin Ala Ala Phe 245 250 255 Trp Ala Ser Lys Trp Ala Gin Gin Asn Gly Lys Leu Ser Thr lie Thr 260 265 270 Thr Thr Leu Ser Lys Ala Ala Lys Leu Gly Asp Tyr Leu Arg Tyr Ala 275 280 285 Met Phe Asp Lys Tyr Phe Lys Lys lie Gly Asn Cys Val Gly Pro Trp 290 295 300 Ser Cys Gin Ala Gly Trp Gly Lys Asp Ser Ala His Tyr Leu Leu Gly 305 310 315 320 Trp Tyr Phe Ala Trp Gly Gly Ser Leu Asp Thr Gin Asn Gly Trp Ala 325 330 335 Trp Arg lie Gly Asp Gly Ala Ala His Phe Gly Tyr Gin Asn Pro Leu 340 345 350 Ala Ala Tyr Ala Leu Val Asn Glu Gin Asn Met Arg Pro Lys Gly Ala 355 360 365 Thr Ala Val Thr Asp Trp Gin lie Ser Leu Asp Arg Gin Leu Glu Phe 370 375 380 Tyr Glu Trp Leu Gin Thr Ser Glu Gly Ala Phe Ala Gly Gly Ala Thr 385 390 395 400 Asn Ser Trp Asn Gly Arg Tyr Asp Thr Pro Pro Ser Asn Leu Thr Ala 405 410 415 Asn Thr Phe His Gly Met Phe Tyr Asp Trp Glu Pro Val Tyr His Asp 420 425 430 Pro Pro Ser Asn Arg Trp Phe Gly Met Gln Pro Trp Ser Val Asp Arg 435 440 445 Leu Ala Gln Leu Tyr Tyr Val Ser Asp Asn Ser Arg Ala Lys Ser Met 450 455 460 Leu Glu Lys Trp Val Lys Trp Val Leu Ser Glu Ile Thr Phe Asn Gly 465 470 475 480 Asn Lys Tyr Ser Ile Pro Ala Thr Leu Glu Trp Ser Gly Val Pro Pro 485 490 495 Asn Val His Val Lys Val Thr Ala His Thr Asn Asp Val Gly Thr Ala 500 505 510 Ser Ala Thr Ala Arg Thr Leu Ala Tyr Tyr Ala Ala Lys Ser Gly Asp 515 520 525 Thr Asn Ala Lys Asn Val Ala Lys Lys Leu Leu Asp Gly Met Trp Asp 530 535 540 Leu Tyr Gln Thr Asn Lys Gly Val Ser Asn Ser Glu Ile Ala Asp Thr 545 550 555 560 Tyr Gly Gln Phe Lys His Glu Val Tyr Val Pro Asn Gly Trp Tyr Gly 565 570 575 Gln Tyr Pro Asn Gly Asp Val Ile Gln Ala Pro Ala Thr Phe Ile Gly 580 585 590 Ile Arg Ser Trp Tyr Lys Lys Asp Pro Gln Trp Ser Lys Val Glu Gln 595 600 605 His Leu Asn Gly Gly Pro Ala Pro Glu Phe Thr Phe His Arg Phe Trp 610 615 620 Ala Gln Ala Asp Val Ala Leu Ser Gln Gly Thr Tyr Gly Met Leu Phe 625 630 635 640 Asn Glu <210> 19 <211> 638 <212> PRT <213> Gastrophysa viridula <400> 19 Met Lys Ser Leu Ala Ile Ala Ile Val Val Cys Leu Phe Gly Gln Leu 1 5 10 15 His Gly Asp Ser Glu Tyr Lys Ala Arg Phe Leu Gln Gln Tyr Ala Lys 25 30 Met His Asp Ser Ala Asn Gly Tyr Phe Ser Lys Trp Gly Ile Pro Tyr 40 45 His Ser Val Glu Thr Leu Met Val Glu Ala Pro Asp Tyr Gly His Glu 55 60 Thr Thr Ser Glu Ala Tyr Ser Tyr Trp Ile Trp Leu Glu Ala Met Tyr 70 75 80 Gly Lys Ile Ser Gly Asn Phe Thr Gly Phe Asn Ala Ala Trp Gln Asn 90 95 Met Glu Gln Tyr Ile Ile Pro Val His Ala Ser Gln Thr Gly Asn Asn 105 110 Tyr Gln Pro Trp Lys Pro Ala Thr Tyr Ser Asp Glu Leu Asp Trp Pro 120 125 Ser Glu Tyr Pro Ser Pro Met Gln Phe Gly Val Pro Val Gly Gln Asp 135 140 Pro lie Tyr Gln Glu Leu Val Asn Thr Tyr Gly Ser Asp Asp Val Tyr 150 155 160 Ser Met His Trp Leu Leu Asp Val Asp Asn Val Tyr Gly Phe Gly Asn 170 175 Leu Pro Gly Ser Cys Glu Leu Gly Pro Gin Ala Asp Gly Pro Ser Tyr 185 190 lie Asn Thr Phe Gin Arg Gly Pro Gin Glu Ser Val Trp Arg Thr lie 200 205 Pro Gin Thr Thr Cys Asp Ser Phe Lys Tyr Gly Gly Gin His Gly Phe 210 215 220 Leu Asp Leu Phe Val Gly Asp Asn Ser Tyr Thr Gin Gin Trp Lys Tyr 225 230 235 240 Ser Asn Ala Pro Asp Ala Asp Ala Arg Ala Val Gin Ala Ala Phe Trp 245 250 255 Ala His Thr Trp Ala Asn Glu Giu Giy Lys Ser Ser Giu Val Ser Asp 260 265 270 Thr Val Ala Lys Ser Ala Lys Leu Gly Asp Tyr Leu Arg Tyr Ala Met 275 280 285 Phe Asp Lys Tyr Phe Lys Lys lie Gly Asn Cys Val Gly Pro Tyr Asn 290 295 300 Cys Ala Gly Gly Gin Gly Lys Glu Ser Ala His Tyr Leu lie Ser Trp 305 310 315 320 Tyr Phe Ala Trp Gly Ala Ala Leu Trp Gly Gly Trp Ser Trp Arq lie 325 330 335 Gly Asp Gly Ala Ala His Phe Gly Tyr Gin Asn Pro Leu Ala Ala Tyr 340 345 350 Ala Leu Ser Asn Val Asp Ala Met Lys Pro Lys Ser Ala Thr Gly Ala 355 360 365 Ser Asp Trp Gin Ala Ser Leu Glu Arg Gin Leu Glu Phe Tyr Ala Trp 370 375 380 Leu Gin Ser Ala Glu Gly Ala lie Ala Gly Gly Ala Thr Asn Thr Trp 385 390 395 400 Asn Gly Arg Tyr Gly Thr Pro Pro Asp Asn Val Thr Lys Asn Thr Phe 405 410 415 His Gly Met Phe Tyr Asp Trp Glu Pro \Tal Tyr His Asp Pro Ala Ser 420 425 430 Asn Arg Trp Tyr Gly Met Gln Pro Trp Ser Leu Asp Arg Leu Ala Gln 435 440 445 Tyr Tyr Tyr Val Ser Gly Asp Gln Lys Ala Lys Ala Ile Leu Asp Lys 450 455 460 Trp Val Ser Trp Ile Leu Ser Glu Leu Gln Phe Asp Asn Gly Asp Tyr 465 470 475 480 Lys Val Pro Glu Trp Leu Glu Trp Ser Gly Val Pro Pro Glu Val His 485 490 495 Val Thr Val Thr Arg Arg Ser Ser Asp Val Gly Thr Ala Ser Ala Thr 500 505 510 Ala Arg Thr Leu Ser Tyr Tyr Ala Ala Lys Ser Gly Asp Thr Asn Ala 515 520 525 Lys Asn Ala Ala Lys Gln Leu Leu Asp Gly Val Trp Lys His Gln Thr 530 535 540 Thr Lys Gly Val Ser Val Glu Glu Val Val Asp Ser Tyr Ser Arq Phe 545 550 555 560 Asn Glu Glu Val Tyr Val Pro Tyr Asn Trp Lys Gly Tyr Tyr Pro Asn 565 570 575 Gly Asp Val Ile Glu His Gly Ala Thr Phe Ile Gly Leu Arg Ser Phe 580 585 590 Tyr Lys Asn Asp Pro Asp Trp Ser Lys Val Glu Ala His Leu Asn Gly 595 600 605 Gly Glu Ala Pro Lys Phe Thr Tyr His Arg Phe Trp Ala Gln Ser Asp 610 615 620 Val Ala Leu Ala Gln Gly Thr Tyr Gly Ile Leu Phe Asn Glu 625 630 635 <210> 20 <211> 639 <212> PRT <213> Gastrophysa viridula <400> 20 Met Lys Ser Ser Ala Ile Ala Leu Phe Val Cys Leu Phe Gly Gln Leu 1 5 10 15 Gln Gly Asp Gly Glu Tyr Lys Ala Arg Phe Leu Oln Gln Tyr Eis Lys 25 30 Ile His Asp Pro Thr Asn Gly Tyr Tyr Ser Lys Gln Gly Val Pro Tyr 40 45 His Ser Ala Glu Thr Leu Met Val Asp Ile Thr Asp His Gly His Glu 55 60 Ser Thr Ser Glu Ala Tyr Ser Tyr Trp Ile Trp Met Glu Ala Met Tyr 70 75 80 Gly Lys Leu Thr Gly Asn Phe Ser Ser Phe Asn Ala Ala Trp Glu Asn 90 95 Met Glu Gln Asn Ile Ile Pro Val His Ala Ser Gln Ile Gly Asn Asn 105 110 Asn Tyr Asn Pro Ser Lys Pro Ala Thr Tyr Ala Asp Glu Leu Asp Trp 120 125 Pro Ser Asp Tyr Pro Ile Pro Leu Gln Phe Asp Val Pro Val Gly Gln 135 140 Asp Pro Ile Phe Gln Glu Leu Val Asp Thr Tyr Gly Ser Tyr Asp Ile 150 155 160 Tyr Ala Met His Trp Leu Leu Asp Ala Asp Asn Val Tyr Gly Phe Gly 170 175 Asn Leu Pro Gly Ser Cys Glu Leu Gly Pro Glu Ala Asp Gly Pro Ser 185 190 Tyr Ile Asn Thr Phe Glu Arq Gly Pro Glu Glu Ser Thr Trp Arq Thr 200 205 lie Pro Gin Pro Thr Cys Asp Thr Phe Lys Tyr Giy Giy Gin His Giy 210 2i5 220 Phe Ser Giu lie Phe Aia Lys Gin Ser Ser Phe Thr Lys Ser Trp Lys 225 230 235 240 Tyr Ser Asn Aia Pro Asp Aia Asp Aia Arg Aia lie Gin Aia Aia Phe 245 250 255 Trp Aia His Thr Trp Aia Asn Giu Giu Giy Lys Ser Phe Giu lie Ser 260 265 270 Ser Thr lie Giu Lys Ala Aia Lys Leu Giy Asp Tyr Leu Arg Tyr Aia 275 280 285 Met Phe Asp Lys Tyr Phe Lys Arg lie Giy Asn Cys Val Giy Pro His 290 295 300 Asn Cys Pro Giy Giy Gin Giy Arg Glu Ser Ala His Phe Leu Leu Ser 305 310 315 320 Trp Tyr Phe Giy Trp Gly Gly Ala Leu Ser Gly Glu Trp Thr Trp Arg 325 330 335 lie Gly Asp Giy Thr Ala Phe Phe Gly Tyr Gin Asn Pro Met Ala Aia 340 345 350 Tyr Ala Leu Ser Asn Val Thr Ala Met Lys Pro Lys Ser Ala Thr Gly 355 360 365 Ala Ser Asp Trp Gin Ala Ser Leu Asp Arg Gin Leu Glu Phe Tyr Gly 370 375 380 Trp Leu Gin Ser Glu Glu Gly Ala lie Ala Gly Gly Val Thr Asn Ser 385 390 395 400 Trp Asn Gly Arg Tyr Asp Thr Pro Pro Glu Asn Leu Thr Glu Asn Thr 405 410 415 Phe His Gly Met Phe Tyr Asp Trp Gin Pro Val Cys His Asp Pro Pro 420 425 430 Thr Asn Arg Trp Tyr Gly Met Gin Pro Trp Ser Leu Asp Arg Leu Ala 435 440 445 Gin Tyr Tyr Tyr Val Thr Gly Asp Glu Lys Ala Lys Thr Val Leu Asp 450 455 460 Lys Trp Val Asn Trp lie lie Ser Glu lie Glu Phe Lys Asn Gly Asn 465 470 475 480 Phe Gin Leu Pro Giu Trp Leu Asp Trp Ser Giy Vai Pro Pro Asn Vai 485 490 495 His Val lie Val Ala Lys Arg Ser Ser Asp Thr Ala Thr Ala Ser Ala 500 505 510 Thr Ala Arq Thr Leu Ser Tyr Tyr Ala Ala Lys Thr Gly Asp Gin Asn 515 520 525 Val Lys Asn Val Ala Lys Gin Leu Leu Asp Cys lie Trp Asn His Gin 530 535 540 Thr Thr Lys Gly Val Ser Val Glu Glu Val Leu Asp Ser Tyr Ser Arg 545 550 555 560 Phe Asn Glu Thr Val Tyr Val Pro Glu Asn Phe Lys Gly Tyr Tyr Pro 565 570 575 Asn Gly Asp Val lie Glu His Gly Ala Thr Phe lie Ser Leu Arq Ser 580 585 590 Phe Tyr Lys Arg Asp Pro Glu Trp Tyr Lys lie Glu Ala Tyr Leu Asn 595 600 605 Gly Gly Glu lie Pro Arg Phe Thr Tyr His Arg Phe Trp Ala Gin Ser 610 615 620 Asp Val Ala Leu Ala Gin Gly Thr Tyr Gly lie Leu Phe Asn Glu 625 630 635 <210> 21 <211> 641 <212> PRT <213> Leptinotarsa decemlineata <400> 21 Met Leu Leu Pro Leu Ala Ala Leu Leu Met Gly Cys Val Ala Gln Ile 1 5 10 15 His Ala Gly Val Tyr Ser Asp Arg Phe Leu Gln Gln Tyr Asn Lys Ile 25 30 His Asp Pro Lys Asn Gly Tyr Phe Ser Lys Glu Gly Ile Pro Tyr His 40 45 Ser Val Glu Thr Leu Met Val Glu Gly Pro Asp His Gly His Glu Thr 55 60 Thr Ser Glu Ala His Ser Tyr Tyr Ile Trp Leu Glu Ala Met Tyr Gly 70 75 80 Lys Leu Thr Asn Asp Phe Ser Lys Phe Asn Thr Ala Trp Gln Ile Met 90 95 Glu Glu Phe Thr Ile Pro Val His Asp Ser Gln Pro Thr Asn Tyr Lys 105 110 Tyr Asn Pro Ser Lys Pro Ala Thr Phe Asn Pro Glu Leu Asp Ser Pro 120 125 Asp Gln Tyr Pro Thr Pro Ser Gln Pro Glu Val Pro Val Gly Gln Asp 135 140 Pro Leu Phe Gln Glu Leu Gln Asn Ala Tyr Gly Thr Pro Asp Met Tyr 150 155 160 Ser Met His Trp Leu Thr Asp Val Asp Asn Val Tyr Gly Phe Gly Asn 170 175 Ser Pro Gly Asn Cys Leu Ala Gly Pro Gly Thr Pro Gly Pro Ser Tyr 185 190 Ile Asn Thr Tyr Gln Arg Gly Ser Gln Glu Ser Thr Trp Lys Thr Ile 200 205 Pro Gln Pro Thr Cys Asp Asn Phe Arg Tyr Gly Gly Arg Asn Gly Phe 210 215 220 Leu Asp Leu Phe Val Arg Asp Asn Asn Tyr Val Gln Gln Trp Lys Tyr 225 230 235 240 Thr Ser Ala Pro Asp Ala Asp Ala Arg Ala Val Gln Ala Ala Phe Trp 245 250 255 Ala Ser Lys Trp Ala Thr Glu Asn Gly Gln Leu Asn Thr Ile Ser Ser 260 265 270 Thr Leu Ala Lys Ala Gly Lys Leu Gly Asp Tyr Leu Arg Tyr Ser Phe 275 280 285 Phe Asp Lys Tyr Phe Lys Arg Ile Gly Asn Cys Val Ser Pro Tyr Asn 290 295 300 Cys Pro Gly Gly Tyr Gly Lys Glu Ser Ala His Tyr Leu Leu Ser Trp 305 310 315 320 Tyr Val Gly Trp Gly Gly Ser Leu Ser Asp Tyr Asn Gly Trp Ala Trp 325 330 335 Arg Ile Gly Asp Gly Ala Ala His Phe Gly Tyr Gln Asn Pro Leu Ala 340 345 350 Ala Tyr Ala Leu Val Asn Asp Tyr Asn Leu Arg Pro Arg Gly Ala Thr 355 360 365 Ala Val Asn Asp Trp Gln Gln Ser Leu Ser Arq Gln Leu Glu Phe Tyr 370 375 380 Gly Trp Leu Gln Ser Gln Glu Gly Ala Phe Ala Gly Gly Ala Thr Asn 385 390 395 400 Ser Trp Asn Gly Arg Tyr Asp Asn Pro Pro Ser Glu Leu Thr Ala Asn 405 410 415 Thr Phe His Gly Met Phe Tyr Asp Trp Glu Pro Val Tyr His Asp Pro 420 425 430 Pro Ser Asn Arg Trp Tyr Gly Met Gln Ala Trp Ser Val Asp Arq Leu 435 440 445 Ala Gln Tyr Tyr Tyr Val Ser Gly Asp Tyr Gln Ala Lys Ser Leu Leu 450 455 460 Asp Lys Trp Val Ser Trp lie Leu Ser Glu lie Lys Phe Asn Gly Asp 465 470 475 480 Asn Tyr Thr Leu Pro Ala Thr Leu Glu Trp Asp Gly Val Pro Pro Asn 485 490 495 Val Arg Val Arg Val Ser Gly Tyr Thr Asn Asp Val Gly Thr Ala Ser 500 505 510 Gly Thr Ala Arg Thr Leu Ser Phe Tyr Ala Ala Lys Ala Asn His Ala 515 520 525 Glu Ala Lys Asn Val Ala Lys Lys Leu Leu Asp Giy Met Trp Lys Leu 530 535 540 Tyr G]n Thr Asp Lys G]y Va] Ser Thr Pro G]u G]u A]a Ser Thr Tyr 545 550 555 560 Asn Gin Phe Asn Leu Pro lie Ala Val Pro Tyr Gly Trp Thr Gly Arg 565 570 575 Tyr Pro Asn Gly Asp Val lie Asp Asn Ser Ala Thr Phe lie Lys Leu 580 585 590 Arg Ser Trp Tyr Lys Asn Asp Pro Asp Trp Pro Lys Val Glu Ala His 595 600 605 Leu Asn Giy Giy Pro Ala Pro Vai Phe Thr Tyr His Arg Phe Trp Ala 610 615 620 Gin Ala Asp His Ala Leu Ala Gin Gly Thr Tyr Gly Leu Leu Phe Asn 625 630 635 640 Glu <210> 22 <211> 643 <212> PRT <213> Leptinotarsa decemlineata <400> 22 Met Leu Leu Giy Vai Met Lys Thr Phe lie Vai Phe Leu Vai Giy Cys 1 5 10 15 Vai Ser Leu lie Asn Giy Giy Giu Tyr Leu Asn Arg Phe Thr Gin Gin 25 30 Tyr Asn Lys lie His Asp Aia Aia Asn Giy Tyr Phe Ser Pro Lys Giy 40 45 lie Pro Tyr His Aia Vai Giu Thr Leu lie Vai Giu Aia Pro Asp Tyr 55 60 Giy His Gin Thr Thr Ser Giu Aia Tyr Ser Tyr Trp Leu Trp Leu Giu 70 75 80 Aia Met Tyr Giy Aia Vai Asn Giy Asp Phe Ser Lys Phe Thr Aia Aia 90 95 Trp Gin Asn Met Giu Thr Tyr Met lie Pro Asn His Aia Ser Gin Pro 105 110 Thr Asn Asn Tyr Tyr Asn Pro Giy Lys Pro Aia Thr Phe Aia Pro Giu 120 125 Giy Asp Tyr Pro Asn Gin Tyr Pro Ser Gin Met Gin Phe Asn Vai Pro 135 140 Vai Gly Gin Asp Pro lie Tyr Gin Giu Leu Vai Asn Thr Tyr Giy Thr 150 155 160 Ser Asp Vai Tyr Leu Met Asn Trp Leu Leu Asp Vai Asp Asn Lys Tyr 170 175 Giy Phe Giy Asn Gin Pro Giy Gin Cys Gin Leu Giy Pro Ser Vai Giu 185 190 Giy Pro Ser Phe Met Asn Ser Phe Gin Arg Giy Pro Gin Giu Ser Vai 200 205 Trp Arg Thr lie Pro Gin Thr Thr Cys Asp Asn Phe Arg Phe Giy Giy 210 215 220 Gin Asn Giy Phe Leu Asp Leu Phe Vai Giy Asp Ser His Tyr Giu Gin 225 230 235 240 Gin Trp Lys Tyr Thr lie Aia Ser Asp Aia Giu Aia Arg Aia lie Gin 245 250 255 Ala Ala Phe Trp Ala Leu Gln Trp Ala Lys Asp Lys Asn Gln Gln Gly 260 265 270 Ala Val Ser Asp Thr Ile Ser Lys Ala Ser Lys Met Gly Asp Phe Leu 275 280 285 Arg Tyr Ala Phe Phe Asp Lys Tyr Phe Lys Lys Ile Gly Asn Cys Ile 290 295 300 Gly Thr Tyr Ala Cys Pro Gly Gly Tyr Gly Lys Asp Ser Ala His Tyr 305 310 315 320 Leu Leu Ser Trp Tyr Met Ala Trp Gly Gly Ser Leu Tyr Gly Asn Trp 325 330 335 Ala Trp Arg Ile Ser Asp Phe Tyr Ala His Phe Gly Tyr Gln Asn Pro 340 345 350 Met Thr Ala Tyr Val Met Ser Lys Val Asp Glu Phe Lys Pro Arg Ser 355 360 365 Pro Thr Gly Val Ser Asp Trp Glu Met Ser Leu Glu Arg Gln Ile Glu 370 375 380 Phe Tyr Glu Tyr Leu Gln Ser Lys Glu Gly Ala Phe Ala Gly Gly Ala 385 390 395 400 Thr Asn Ser Tyr Glu Gly Arg Tyr Glu Thr Pro Pro Ala Asn Leu Met 405 410 415 Asn Asn Thr Phe His Gly Met Trp Tyr Glu Trp Glu Pro Val Tyr His 420 425 430 Asn Pro Pro Ser Asn Arg Trp Phe Gly Met Gln Ala Trp Ser Thr Asp 435 440 445 Arg Leu Ala Gln Tyr Tyr Tyr Val Ser Gly Asp Glu Lys Val Arq Pro 450 455 460 Leu Leu Asp Lys Trp Val Asn Trp Val Leu Pro Glu Ile Ser Tyr Asp 465 470 475 480 Ala Asn Ser Tyr Lys lie Pro Glu Trp Leu Asp Trp Ser Gly Val Pro 485 490 495 Pro Ala Val Asp Val Ser lie Glu Lys Tyr Gly Thr Asp lie Gly Ser 500 505 510 Ala Ser Ala Leu Ala Arg Thr Leu Ser Tyr Tyr Ala Ala Lys Thr Gly 515 520 525 Asp Gin Lys Val Lys Ala Val Ala Lys Gly Leu Leu Asp Gly lie Trp 530 535 540 Asn Asn His Gin Thr Thr Lys Gly Val Ala Met Glu Glu Val Met Asp 545 550 555 560 Ala Tyr Ser Gin Phe Asn Glu Glu Val Tyr Val Pro Pro Gly Trp Val 565 570 575 Gly His Tyr Pro Asp Gly Thr Glu lie Lys Ala Pro Thr Thr Phe lie 580 585 590 Gly lie Arg Pro Trp Tyr Lys Asn Asp Pro Asp Trp Pro Lys Val Glu 595 600 605 Ala Tyr Leu Asn Gly Gly Pro Ala Pro Lys Phe Thr Tyr His Arq Phe 610 615 620 Trp Gin Gin Cys Asp lie Ala lie Ala Gin Gly Thr Tyr Gly Leu Leu 625 630 635 640 Phe Asn Glu <210> 23 <211> 640 <212> PRT <213> Leptinotarsa decemlineata <400> 23 Met Lys Leu Leu Ser lie Ala Leu Ala Leu Ala Gly Leu Thr Leu Val 1 5 10 15 Asn Cys Ala Gly Val Tyr Lys Gin Arg Phe Leu Glu Gin Tyr Asn Lys 25 30 lie His Asn Glu Asn Asn Gly Tyr Phe Ser Lys Glu Gly Val Pro Tyr 40 45 His Ala Val Glu Thr Leu lie lie Glu Ala Cys Asp His Gly His Gin 55 60 Thr Thr Ser Glu Ala Tyr Ser Tyr Tyr lie Trp Leu Glu Ala Met Asn 70 75 80 Gly Ala lie Ser Gly Asn Phe Ser Ser Phe Asn Val Ala Trp Lys Asn 90 95 Met Glu Asp Tyr lie lie Pro Ser His Thr Ser Gin Pro Asn Thr Asp 105 110 Leu Tyr Asn Pro Lys Asn Pro Ala Thr Tyr Ala Pro Ala Met Asp Thr 120 125 Pro Glu Gin Trp Pro Thr Ser Leu Glu Pro Gly Val Pro Val Gly Glu 135 140 Asp Pro Leu Phe Gin Glu Leu Gin Thr Thr Tyr Asp Thr Tyr Asp Val 150 155 160 Tyr Ala Leu His Trp Leu Leu Asp Val Asp Asn Thr Tyr Gly Phe Giy 170 175 Asp Ser Gin Gly Thr Cys Ser Gly Gly Pro Ser Val Lys Lys Pro Ser 185 190 Phe lie Asn Asn Tyr Gly Arg Gly Pro Ser Glu Asn Val Trp Met Ala 200 205 lie Pro Ser Gly lie Cys Glu Ala Phe Lys Tyr Gly Ser Lys Thr Gly 210 215 220 Phe Ser Gly lie Tyr Ser Lys Giy Asn Pro Val Pro Asn Trp Lys Phe 225 230 235 240 Ser Val Ala Pro Asp Ala Asp Aia Arg Ala lie Leu Giy Ala Phe Trp 245 250 255 Ala Ser Arq Trp Ala Thr Glu Lys Gly Glu Leu Ser Gin lie Thr Asp 260 265 270 Thr Leu Ala Lys Ala Ser Lys Leu Gly Asp Tyr Leu Arg Tyr Ala Met 275 280 285 Phe Glu Lys Tyr Phe Lys Arg Val Gly Asn Cys lie Asp Pro Tyr Lys 290 295 300 Cys Pro Gly Gly His Asp Arg Glu Ser Ala His Tyr Leu Leu Ser Trp 305 310 315 320 His Val Gly Trp Gly Gly Ser Val Ser Arg Asn Gly Gly Tyr Ala Trp 325 330 335 Arg Val Ala Asp Ala Thr Val His Phe Gly Tyr Gin Asn Pro Leu Ala 340 345 350 Ala Tyr Ala Leu Ser Gin Glu Pro Thr Leu Lys Pro Lys Ser Pro Thr 355 360 365 Ala Ala Gin Asp Trp Ala Ala Ser Leu Glu Arg Gin Leu Glu Phe Tyr 370 375 380 Glu Tyr Val Gin Ser Ala Glu Gly Ala Phe Gly Gly Gly Val Val Asn 385 390 395 400 Ser Trp Lys Asp Arg Tyr Asp Thr Pro Pro Asp Asp lie Ala Ser Ser 405 410 415 Thr Phe His Gly Met Phe Tyr Asp Trp Gin Pro Gly Gly His Asn Pro 420 425 430 Pro Ser Thr Arg Trp Phe Gly Met Gin Pro Trp Ser Thr Asp Arg Leu 435 440 445 Ala Gin Tyr Tyr Tyr Val Thr Gly Asp Lys Lys Ala Lys Ala lie Leu 450 455 460 Asp Lys Trp Val Ser Trp Val lie Ser Glu lie Thr Phe Glu Gly Asp 465 470 475 480 Asn Tyr Lys Leu Pro Asn Ser Leu Glu Trp Ser Gly Val Pro Pro Asn 485 490 495 Val His Val Lys Val Ala Asp Tyr Gly Ser Gly Ile Gly Pro Ala Ala 500 505 510 Ala Thr Ala Arg Thr Leu Ser Tyr Tyr Ala Ala Lys Ser Gly Asn Lys 515 520 525 Ala Ala Lys Gln Val Ala Lys Lys Leu Leu Asp Gly Met Trp Lys Leu 530 535 540 Tyr Arg Thr Asn Leu Gly Val Ser Ser Glu Glu Gln Pro Asp Tyr Asp 545 550 555 560 His Phe Gln Asp Lys Val Tyr Val Pro Pro Gly Trp Ser Gly Val Tyr 565 570 575 Pro Asn Gly Asp Val Leu Asn Ser Ser Ser Thr Phe Leu Ser Ile Arg 580 585 590 Ser Phe Tyr Lys Lys Asp Pro Gln Trp His Lys Ile Glu Ala Arg Leu 595 600 605 Asn Gly Gly Pro Ala Pro Thr Phe Thr Tyr His Arg Phe Trp Asp Gln 610 615 620 Ser Glu Val Ala Leu Ala Met Gly Ala Tyr Gly Leu Leu Phe Asn Glu 625 630 635 640 <210> 24 <211> 637 <212> PRT <213> Sitophilus oryzae <400> 24 Met Arg Val Ser Leu Ala Phe Leu Ala Ile Val Cys Val Val Gly Ile 1 5 10 15 His Gly Gly Thr Tyr Thr Asp Arg Phe Leu Glu Gln Tyr Asn Lys Ile 25 30 His Asp Ser Ala Asn Gly Tyr Phe Ser Ser Glu Gly Ile Pro Tyr His 40 45 Ser Val Glu Thr Leu Ile Val Glu Ala Pro Asp His Gly His Gln Thr 55 60 Thr Ser Glu Ala Phe Ser Tyr Tyr Val Trp Leu Glu Ala Met Tyr Gly 70 75 80 Ala Val Asn Gly Asp Phe Ser Ser Phe Asn Thr Ala Trp Thr Thr Leu 90 95 Glu Asn Tyr Ala Ile Pro Ala Leu Gln Thr Ala Asn Ala Ala Tyr Asn 105 110 Pro Ser Lys Pro Gly Ser Tyr Glu Ala Glu Leu Asp Asp Pro Ser Asp 120 125 Tyr Pro Ser Pro Ile Asp Phe Asn Val Pro Val Gly Gln Asp Pro Ile 135 140 Ala Thr Glu Leu Lys Asn Ala Tyr Gly Ser Asp Val Leu Tyr Ala Met 150 155 160 His Trp Leu Leu Asp Val Asp Asn Val Tyr Gly Phe Ser Asn Thr Gln 170 175 Gly Gln Cys Glu Asp Gly Thr Asn Gly Pro Ser Leu Ile Asn Asn Tyr 185 190 Gln Arg Gly Pro Gln Glu Ser Val Trp Arg Thr Val Pro Gln Pro Thr 200 205 Cys Asp Gln Phe Lys Tyr Gly Gly Gln Asn Gly Phe Leu Asp Leu Phe 210 215 220 Thr Lys Asp Asn Ser Tyr Ala His Gln Tyr Lys Tyr Ser Ala Ala Pro 225 230 235 240 Asp Ala Asp Ala Arg Ala Val Gln Ala Ala Tyr Trp Ala Ala Gln Trp 245 250 255 Ala Thr Asp Asn Gly Gln Ile Ser Ser Ile Thr Asp Thr Leu Ser Lys 260 265 270 Ala Ala Lys Leu Gly Asp Tyr Leu Arg Tyr Ser Leu Tyr Asp Lys Tyr 275 280 285 Phe Lys Lys Val Gly Asn Cys Val Gly Ala Tyr Ala Cys Gin Gly Gly 290 295 300 Thr Gly Lys Glu Ser Ala His Tyr Leu lie Ser Trp Tyr Phe Ala Trp 305 310 315 320 Gly Ala Ser Tyr Asn Ala Gin Tyr Asp Trp Ala Trp Arg lie Gly Asp 325 330 335 Gly Ala Ala His Phe Gly Tyr Gln Asn Pro Leu Ala Ala Tyr Ala Leu 340 345 350 Ser Thr Asp Ser Asn Leu Thr Pro Lys Gly Ser Thr Ala Val Glu Asp 355 360 365 Trp Lys Thr Ser Leu Asp Arg Gin Leu Glu Leu Tyr Glu Tyr Leu Gin 370 375 380 Thr Ser Glu Gly Ala Phe Ala Gly Gly Val Thr Asn Ser Trp Lys Gly 385 390 395 400 Arg Tyr Asp Thr Pro Asp Ser Asp Leu Leu Val Asp Thr Phe His Gly 405 410 415 Met Phe Tyr Asp Trp Glu Pro Vai Tyr His Asp Pro Pro Ser Asn Arg 420 425 430 Trp Tyr Gly Met Gin Pro Trp Ser Ala Asp Arq Leu Ala Gin Tyr Tyr 435 440 445 Tyr Val Thr Gly Asp Thr Lys Ala Gin Ala lie Leu Glu Lys Trp Val 450 455 460 Asn Trp Val lie Pro Asn lie Lys Trp Thr Gly Asp Asp Phe Glu Met 465 470 475 480 Pro Ala Asn Leu Gly Trp Thr Giy Asp Pro Pro Asn Ala His Val Asn 485 490 495 Val Thr Ser Trp Giy Lys Asp Leu Gly Thr Ala Ser Ala Thr Ala Arg 500 505 510 Ala Phe Ser Tyr Tyr Ala Ala Lys Ala Asn Asp Ala Thr Val Lys Glu 515 520 525 Asn Ala Lys Lys lie Leu Asp Ala Leu Tyr Ala Asn Tyr Gin Thr Thr 530 535 540 Lys Gly lie Ser Ala Pro Glu Thr Arg Glu Asp Tyr Ser Arg Phe Asn 545 550 555 560 Glu Ala Val Tyr Val Pro Ser Gly Trp Thr Gly Thr Tyr Pro Asn Gly 565 570 575 Asp Val lie Asp Ser Ser Ala Thr Phe lie Lys lie Arg Ser Trp Tyr 580 585 590 Lys Asn Asp Pro Asp Trp Ser Lys lie Gin Ala Tyr Leu Asp Gly Gly 595 600 605 Asp Val Pro Thr Phe Thr Tyr His Arg Phe Trp Thr Gin Ala Asp lie 610 615 620 Ala Leu Ala Phe Gly Ala Tyr Gly Leu Leu Phe Asn Glu 625 630 635 <210> 25 <211> 370 <212> PRT <213> Chrysomela tremuiae <400> 25 Met Phe Ser Leu Arg Leu Phe Ser Val lie Ala Thr Ala Phe Ala Ala 1 5 10 15 Val Ser Ala Leu Pro Thr Gin Asn Glu Thr Leu Ser Leu Gly Gly Ser 25 30 Cys Thr lie Lys Glu Phe Ser Gin Val Ser Ser Val lie Ser Ser Cys 40 45 Thr Asn lie Val lie Ser Gly Leu Ser Val Pro Gly Gly Lys Thr Leu 55 60 Asp Leu Lys Leu Lys Lys Gly Thr Thr Leu Thr Phe Glu Gly Lys Thr 70 75 80 Val Phe Gly Val Ala His Trp Asp Gly Pro Leu Leu Gin lie Ser Gly 90 95 Giu Lys lie Thr Val Lys Gly Ala Ser Gly Ser Vai Leu Asp Gly Gin 105 110 Gly Ala Lys Tyr Trp Asp Gly Lys Gly Asp Gly Gly Val Lys Lys Pro 120 125 Lys Phe Val Arg Val Lys Ala Ser Gly Gly Ser Val Leu Thr Gly Leu 135 140 His Val Leu Asn Cys Pro Arq Gln Cys Val Ser Val Asn Ser Cys Asp 150 155 160 His Va] Thr Leu Ser Asn Trp Asn Va] Asp Va] Ser A]a G]y Asp Lys 170 175 Asn Lys Leu Giy His Asn Thr Asp Gly Phe Asp lie Ser Ser Thr Ser 185 190 Asn Leu Leu lie Glu Asn Ser Val Val Lys Asn Gln Asp Asp Cys Val 200 205 Ala Val Asn Lys Ala Gln His Val Thr lie Ser Lys Leu Gln Cys Ser 210 215 220 Gly Gly His Gly Leu Ser Leu Ser Val Gly Met Ser Lys Thr Ser Ala 225 230 235 240 Glu Ala Asn Vai Vai Lys Asp Vai His Phe Vai Asp Cys Thr Vai Thr 245 250 255 Asn Ser Asp Asn Giy lie His lie Lys Thr His Ser Asp Giy Ala His 260 265 270 Giy Thr lie Asp Asn Vai Ser Tyr Lys Asn lie His Leu Ser Giy Tie 275 280 285 Ala Lys Phe Gly Vai Asn lie Gln Gin Asp Tyr Glu Asn Giy His Ser 290 295 300 Thr Giy Lys Pro Lys Asn Asn lie Pro lie Thr Giy Leu Ser lie Thr 305 310 315 320 Gly Val Thr Gly Ser Met Thr Gly Ser Asn Ser Lys Ala Val Tyr Ile 325 330 335 Leu Cys Ala Ser Gly Gly Cys Ser Gly Trp Asn Trp Gly Val Ser Ile 340 345 350 Ser Gly Ala Lys Lys Ala Asn Ser Cys Thr His Gln Pro Ser Asn Val 355 360 365 Lys Cys <210> 26 <211> 364 <212> PRT <213> Chrysomela tremulae <400> 26 Met Tyr Pro Leu Thr Ile Val Val Ala Ser Gln Leu Val Leu Ala Gly 1 5 10 15 Leu Ala Leu Ala Gln Pro Cys Thr Ile Ser Arg Tyr Ser Asp Ile Gln 25 30 Gln Val Val Asn Ser Cys Thr Asn Ile Leu Val Ser Asn Leu Thr Met 40 45 Pro Ala Gly Ile Val Leu Glu Leu Tyr Leu Gln Asn Lys Thr Thr Ile 55 60 Thr Phe Glu Gly Thr Thr Leu Phe Glu Tyr Thr Pro Trp Ser Gly Pro 70 75 80 Leu Ile Arg Val Thr Gly Val Asp Val Thr Phe Glu Gly Ala Pro Gly 90 95 Ser Val Phe Asp Gly Gln Gly Pro Leu Tyr Trp Asp Gly Arg Gly Asp 105 110 Lys Gly Pro Lys Lys Pro Gln Phe Met Lys Ile Glu Val Phe Gly Lys 120 125 Ser Val Phe Arg Asn Ile Lys Leu Leu Asn Cys Pro His His Cys Val 135 140 Tyr lie Gly Lys Ser Asp Gly Leu Thr Val Ser Asn Trp Thr lie Asp 150 155 160 Asn Ser Ala Gly Asp Leu His Asn Phe Thr Gly His Asn Thr Asp Gly 170 175 Phe Asp Val Ser Ala Ala Lys Asn Leu Leu lie Glu Asn Thr Ala Val 185 190 Leu Asn Gin Asp Asp Cys lie Ala lie Arg Tyr Gly Ser Asn lie lie 200 205 Val Arq Asn Met Tyr Cys Tyr Gly Gly His Gly Leu Ser Leu Ser Val 210 215 220 Gly Phe Asn His Thr Ser Phe Pro Glu Asn Val Val Glu Asn Val Val 225 230 235 240 lie Glu Asp Ser Leu Leu Val Gly Ser Ala Asn Gly lie His Val Lys 245 250 255 Thr His Thr Asp Gly Tyr Leu Gly Leu Val Arg Asn lie Thr Tyr Arg 260 265 270 Asn lie Glu Met Lys Asp lie Thr Asn Tyr Gly lie Asn Val Gin Gin 275 280 285 Asp Tyr Cys Asn Gly Ser Ser Thr Gly Thr Pro Thr Ser Asn lie Pro 290 295 300 lie Glu Ala Leu Asn lie Tyr Asn Val Thr Ala Ser Ala Gin Lys Gly 305 310 315 320 Ser Lys Ala Met Gly Val Tyr lie lie Cys Gly Glu Asp Ser Cys Arg 325 330 335 Asn Trp Thr Trp Ser Gly lie His Phe Glu Gly Ala Ala Lys Lys Asp 340 345 350 Ser Cys Asn Phe Ser Pro Gin Giy Tyr Lys Cys Arg 355 360 <210> 27 <211> 373 <212> PRT <213> Chrysomela tremuiae <400> 27 Met lie Leu His Leu Lys Ser Phe Val Phe Leu Leu lie Val Val Val 1 5 10 15 Ser lie Ala His Gly Tyr Glu Pro lie Asp Tyr Gin Thr Ala Lys Asn 25 30 Leu Thr Ala Asp Tyr Cys Tyr Val Asn Ser Tyr Asn Glu Val Asp Ser 40 45 lie Val Lys Ser Cys Arg Tyr lie Val lie Gin Gly Phe Glu Val Pro 55 60 Ala Gly Lys Thr Leu Asn Leu Asn Leu Gin Lys Lys Thr Val Leu Leu 70 75 80 Phe Glu Gly Thr Leu Thr Phe Gly Met Ala Tyr Trp Asp Gly Pro Leu 90 95 lie Ser lie Ser Gly Tyr Asp lie Gin Val Met Gly Gly Thr Asn His 105 110 Leu lie His Gly His Gly Glu Lys Tyr Trp Asp Gly Gin Gly Asp Gly 120 125 Gly Val Lys Lys Pro Arg Lys Val Met Thr lie Asn Ala His Gly Gly 135 140 Ser Phe Lys Asn lie Asn lie Lys Asn Cys Pro Leu Phe Cys Val Ala 150 155 160 lie Val Gly Thr Asp Leu Thr Phe Ser Gly Phe Asn lie Asp Leu Gin 170 175 Asp Giy Phe Asn Asn Asn Leu Gly Arg Asn Thr Asp Gly Met Ser Phe 185 190 Ser Gin Ser Asn Asn lie Leu lie Glu Asn Cys Lys lie Trp Asn Gin 200 205 Asp Asp Cys Val Asn Val Leu Gly Gly Ser Thr Asn Ser Leu Val Arg 210 215 220 Asn Val His Cys Trp Gly Ser His Gly Phe Ser lie Thr Ser Gly Met 225 230 235 240 Ala Asp Ser Glu Lys Leu Asn Ser lie Ser Asn lie Thr Phe Glu Asp 245 250 255 Ser Ser lie Gly Gly Ser Leu Gly Gly lie His Val Lys Thr Leu Pro 260 265 270 Thr Gly Gly Pro Gly lie Leu Asp Arg lie Thr Tyr Arg Asn lie Gin 275 280 285 lie Thr Asp Val Thr Arg Phe Ala lie Glu Ala His Gin Asp Tyr Pro 290 295 300 Asn Glu Asp Gly Lys Ser Ser Asn Asn lie Lys lie Thr Asn Leu Asn 305 310 315 320 Leu lie Gly Val Thr Gly Thr Val Lys Gly Ser Ser Thr Arg Ser Val 325 330 335 Arg lie Asn Cys Gly Ser Gly Ser Cys Ser Asn Trp Asn Trp Ser Gly 340 345 350 lie Lys lie Thr Gly Ala Gin Asn Lys Asp Glu Cys Asn Phe His Pro 355 360 365 Asn Gly Tyr Ser Cys <210> 28 <211> 376 <212> PRT <213> Chrysomela tremulae <400> 28 Met lie Ser Leu lie Glu Ala Phe Val Ser Leu Leu Val lie Val Val 1 5 10 15 Ser lie Lys Ala Asp Ser Asn Gly Tyr Glu Pro lie Asp Tyr Gin Thr 25 30 Ala Lys Asn Leu Thr Ala Asp Tyr Cys Tyr Val Lys Ser Tyr Glu Glu 40 45 Val Asp Ser Val Val Gln Asn Cys Asn Arg Ile Val Leu Glu Ser Phe 55 60 Glu Val Pro Ala Gly Lys Thr Leu Thr Leu Lys Leu Arg Lys Lys Ala 70 75 80 Val Leu Leu Phe Glu Gly Thr Leu Thr Phe Gly Met Ala Tyr Trp Asp 90 95 Gly Ser Leu Ile Glu Ile Arq Gly Glu Asn Ile Gln Val Ile Gly Gly 105 110 Thr Asn His Leu Ile His Gly His Gly Glu Lys Tyr Trp Asp Gly Leu 120 125 Gly Val Ala Gly Val Val Lys Pro Lys Arg Leu Met Ser Ile Asn Val 135 140 Lys Gly Gly Thr Phe Thr Asn Ile Asn Ile Lys Asn Cys Pro Val Phe 150 155 160 Cys Val Ala Ile Met Gly Asn Asp Leu Thr Phe Ser Gly Phe Asn Ile 170 175 Asp Leu Gln Asp Gly Phe Lys Asn Ser Leu Gly Arg Asn Thr Asp Gly 185 190 Met Gly Phe Ser His Ala Asn Asn Ile Val Ile Glu Asn Ser Lys Ile 200 205 Thr Asn Gln Asp Asp Cys Val Asn Ala Leu Gly Asp Ser Ser Asn Ala 210 215 220 Val Val Arq Asn Val His Cys Trp Gly Ser His Gly Phe Ser Val Thr 225 230 235 240 Ser Gly Met Ser Thr Thr Asn Thr Gly Asn Asp Ile Arg Asn Ile Thr 245 250 255 Phe Glu Asp Cys Ser lie Giy Giy Ser Leu Giy Giy lie His Vai Lys 260 265 270 Thr Leu Pro Thr Giy Giy His Giy Thr Leu Asp Gin lie Thr Tyr Arg 275 280 285 Asn lie Giu lie Lys Asp Thr Thr Arg Phe Aia lie Giu Vai Gin Gin 290 295 300 Asp Tyr Pro Lys Giu Asp Giy His Ser Ser Asn Asn lie lie lie Asn 305 3i0 3i5 320 Asp Leu Asn Leu lie Asn Vai Thr Giy Ser Vai Lys Giy Lys Ser Aia 325 330 335 Arg Giy Vai Arg lie Ser Cys Giy Ser Giy Ser Cys Thr Asn Trp Lys 340 345 350 Trp Ser Giy Vai Lys lie Thr Giy Aia His Lys Lys Asp Asp Cys Asn 355 360 365 Phe His Pro Asn Giy Tyr Ser Cys 370 375 <2i0> 29 <2ii> 374 <2i2> PRT <2i3> Chrysomeia tremuiae <400> 29 Met lie Pro His Met His Phe Leu Leu Giy Phe Leu lie Vai lie Vai i 5 10 15 Vai Asp lie Aia Aia Thr Thr Thr Ser Tyr Giu Ser Vai Asp Tyr Gin 25 30 Lys Aia Lys Asn Leu Thr Aia Asp Tyr Cys Tyr Aia Arg Ser Tyr Aia 40 45 Giu Vai Pro Asp lie lie Lys Lys Cys Lys Tyr lie Vai lie Vai Asn 55 60 Phe Giu Vai Pro Aia Giy Asn Thr Leu Gin Leu His Leu Gin Lys Asn 70 75 80 Ser Val Leu Leu lie Glu Gly Thr lie Thr Phe Gly Val Ala Asn Trp 90 95 Asp Gly Pro Leu Met Asp lie Ala Gly Glu Asn lie Gin Val lie Gly 105 110 Gly Thr Asn His Leu lie His Gly Lys Gly Glu Lys Tyr Trp Asp Gly 120 125 His Gly Gly Giy Gly Val Lys Lys Pro Lys Arg Leu Met Ser lie Asn 135 140 Val His Arq Giy Ser Phe Thr Asn lie Asn lie Lys Asn Cys Pro Met 150 155 160 Phe Cys Val Ser lie lie Gly Asn Asp Leu Thr Phe Ser Giy Phe Asn 170 175 lie Asp Leu Gin Asp Giy Phe Lys Giy Giy Leu Ala Vai Asn Thr Asp 185 190 Ala Met Giy Phe Vai Tyr Ser Asp Asn lie Leu lie Lys Asp Ser Lys 200 205 lie Trp Asn Gin Asp Asp Cys lie Asn Vai Met Aia Ser Lys Asn Aia 210 215 220 Vai lie Arg Asn Vai His Cys Trp Giy Ser His Giy Leu Ser Leu Ser 225 230 235 240 Ser Giy Leu Ser Lys Thr Asp Giu Asn Asn Asp lie His Asn lie Thr 245 250 255 Tyr Giu Asp Cys Ser lie Giy Giy Ser Leu Asn Giy lie His lie Leu 260 265 270 Thr Met Pro Ser Giy Giy Lys Giy Lys lie Ser Gin lie Thr Tyr Arg 275 280 285 Asn lie Giu lie Lys Giu Vai Thr Met Arq Giy lie Giu Phe Arq Gin 290 295 300 Asp Tyr Leu Asp Asp Gly His Pro Ser Asn Asn lie Lys lie Asp Gly 305 310 315 320 Leu Thr Leu Glu Asn lie His Gly Thr Val Thr Ser Gly Ala Val Giy 325 330 335 Val Tyr lie Gin Cys Giy Ser Giy Ser Cys Thr Asn Trp Asn Trp Ser 340 345 350 Giy Val Lys Met Thr Giy Ser Lys Lys Lys Asp Val Cys Asn Phe His 355 360 365 Pro Glu Giy Tyr Ser Cys <210> 30 <211> 376 <212> PRT <213> Chrysomela tremulae <400> 30 Met lie His Phe Ser Lys Ser Val Val Thr Leu Leu Met Val Leu Val 1 5 10 15 Gly Thr Ser Ala Thr Leu Val Gly Tyr Glu Ser lie Asp Tyr Ala Thr 25 30 Ala Leu Asn Leu Thr Ala Asp Tyr Cys Tyr Ala Arg Ser Tyr Glu Glu 40 45 Val Pro Ser lie Val Gin Arg Cys Glu Tyr lie Val Leu Val Asp Phe 55 60 Glu Val Pro Ala Gly Glu Thr Leu Glu Leu Asn Leu Gin Lys Asn Ser 70 75 80 Val Leu Leu Phe Glu Gly Thr lie Lys Phe Ala Val Ala Tyr Trp Asp 90 95 Gly Pro Leu lie His lie Thr Gly Asn Asn lie Gin Val lie Gly Gly 105 110 Thr Lys His Leu lie His Gly Gin Gly Glu Phe Tyr Trp Asp Gly Gin 120 125 Gly Gly Ser Gly Gly Val Lys Lys Pro Glu Lys Val Phe Ser Val Asn 135 140 Val Asn Gly Gly lie Phe Lys Asn lie Asn lie Lys Asn Cys Pro Thr 150 155 160 Ala Cys Val Lys lie Tyr Gly Asn Asp Leu Thr Phe Ser Gly Phe Asn 170 175 lie Asp Leu Asn Asp Gly Phe Lys Asn Lys Leu Ala Lys His Thr Asp 185 190 Ala Met Thr Phe Ser His Ser Asn Asn lie Val Ile Glu Asn Ser Lys 200 205 lie Val Asn Gin Gly Asp Cys Val Asn Val lie Cys Ser Ser Asn Ala 210 215 220 Val lie Arg Asn Val His Cys Trp Gly Ser His Gly Leu Thr lie Ser 225 230 235 240 Ser Giy Leu Ser Thr Ser Asp Arg Asn Asn Asp Vai Gin Asn lie Thr 245 250 255 Tyr Glu Asp Cys Ser lie Gly Giy Ser Leu Ser Giy lie His Phe Lys 260 265 270 Thr His Pro Ala Giy Gly His Giy Thr Val Asp Gin Vai Thr Tyr Arg 275 280 285 Asn lie Glu lie Lys Asp Val Thr Arg His Gly lie Glu lie Gin Gin 290 295 300 Asp Tyr Pro Asn Asn Asp Gly His Pro Ser Asn Asn lie Lys lie Asn 305 310 315 320 Asn Leu Asn Leu lie Asn Val Thr Gly Thr Val Lys Gly Lys Thr Thr 325 330 335 Arg Gly Val Arg lie Ser Cys Giy Ser Gly Ser Cys Thr Asn Trp Lys 340 345 350 Trp Ser Asp Val Lys lie Thr Gly Ala His Lys Lys Asp Asp Cys Asn 355 360 365 Phe His Pro Asp Gly Tyr Ser Cys 370 375 <210> 31 <211> 366 <212> PRT <213> Chrysomela tremuiae <400> 31 Met Leu Ser Leu Arg Leu lie Ala Leu Leu Ser Ala Ala Ser lie Ala 1 5 10 15 Val Gin Ala Thr Pro Val Val Asp Ser Ser Cys Ala lie Thr Asn Phe 25 30 Asp Gin Val Ala Ser Val Val Asp Lys Cys Thr Asp lie Val lie Ser 40 45 Asn Leu Glu Val Pro Ala Gly Glu Thr Leu Asp Leu Glu Phe Lys Lys 55 60 Thr Gly Val Lys Val Thr Phe Glu Gly Arg Thr Thr Phe Gly Tyr Lys 70 75 80 Glu Trp Lys Gly Pro Leu Leu Arg lie Lys Gly Lys Ala lie Thr Val 90 95 Val Gly Ala Glu Gly Ser Val Leu Asp Gly Gin Gly Gin Leu Tyr Trp 105 110 Asp Gly Lys Gly Gly Asn Gly Gly Lys Asp Lys Pro Lys Phe Phe Lys 120 125 lie Lys Ala Thr Glu Gly Ser His Phe Lys Asn lie Asn Leu Leu Asn 135 140 Cys Pro Val Gin Cys Thr Ser lie Asp His Ser Gly Pro Leu Thr Leu 150 155 160 Ser Gly Trp Asn lie Asp Val Ser Ala Gly Asp Lys Asn Asn Leu Gly 170 175 His Asn Thr Asp Gly Phe Asp lie Asn Thr Thr Asp Gly Leu Val lie 185 190 Glu Asp Ala lie Val Lys Asn Gin Asp Asp Cys lie Ala Val Asn Gin 200 205 Giy Thr Asn Phe Val Phe Ser Asn Leu Glu Cys Tyr Giy Giy lie Giy 210 215 220 Leu Ser Leu Ser Val Giy Thr Ser His Asp lie Leu Lys Asn Thr Val 225 230 235 240 Arg Asn Val Thr Phe Thr Asn Ser Val Vai Ala Lys Ser Arg Asn Gly 245 250 255 lie His lie Lys Thr His Thr Asn Ser Giy Gin Giy lie lie Giu Asp 260 265 270 lie Thr Tyr Ser Asn lie Arg Met Thr Asp lie Trp Lys Tyr Ala Val 275 280 285 Asn lie Glu Gin Asp Tyr Lys Gin Gly Lys Pro Thr Gly His Pro Thr 290 295 300 Asp Asn lie Pro lie Lys Gly Leu Thr Leu Lys Gin Val Ser Gly Thr 305 310 315 320 Leu Thr Gly Asp Glu Ser Val Pro Val Tyr lie lie Cys Ala Asp Gly 325 330 335 Ala Cys Ser Asn Phe Asn Trp Ser Gly Val Ser Phe Thr Gly Ala Ala 340 345 350 His Lys Ser Asn Cys Thr Met lie Pro Thr Gly Phe Ser Cys 355 360 365 <210> 32 <211> 356 <212> PRT <213> Chrysomela tremulae <400> 32 Met Asn Ala lie Phe Gly Leu Leu Leu Ala Thr Ala Ser Thr Ala Phe 1 5 10 15 Ser Phe Ser Leu Asn Cys Thr lie Thr Giu Phe Ser Gin Vai Aia Phe 25 30 Vai Vai Asn Ser Cys Arg Asp lie Vai lie Ser Asp Leu Vai Vai Pro 40 45 Giy Giy Gin lie Leu Giu Leu Asp Leu Leu Aia Giy Thr Arg Vai Thr 55 60 Phe Giu Giy Thr Thr Lys Phe Giu Tyr Tyr Tyr Trp Aia Giy Pro Leu 70 75 80 lie Arg Vai Arg Giy Giu Asn Vai Tyr Phe Arq Giy Aia Giu Giy Ser 90 95 Vai Leu Asp Giy Gin Giy Aia Leu Trp Trp Asp Giy Lys Giy Giy Aia ioo i05 ho Vai Pro Giy Lys Pro Tyr Met lie Giu lie Asp Vai Thr Giy Giy Leu us i20 i25 Phe Giu Asp lie Phe Leu Leu Asn Cys Pro His His Cys Vai lie lie i30 i35 i40 Ser Ser Thr Asp Leu lie Leu Asp Arg Trp Thr Vai Asp Vai Ser Gin i45 iSO i55 i60 Giy Asn lie Lys Asn Leu Giy His Asn Thr Asp Giy Phe Asp lie lie i65 170 i75 Tyr Giy Arg Asn Vai Thr lie Lys Asn Ser Thr Vai Ser Asn Gin Asp i80 i85 190 Asp Cys Vai Aia lie Asn Arg Giy Giu Giy Met Leu lie Thr Gin Met i95 200 205 Asp Cys Thr Giy Ser His Giy Leu Ser lie Ser Vai Giy Phe Ser His 2i0 2i5 220 Arg Ser Phe Leu His Asn Arq Vai Vai Asp Vai lie lie Giu Asn Ser 225 230 235 240 Vai Leu Arg Asp Giy Asp Asn Giy lie His Vai Lys Thr His Thr Asp 245 250 255 Gly Tyr Leu Gly Glu lie Lys Asn lie Thr Tyr Arg Asn lie His Met 260 265 270 Thr Gly lie Arg Asn Phe Gly Ala Glu lie Gin Gin Asn Tyr Pro Asp 275 280 285 Gly Thr lie Lys Pro \7a1 Gly Asn lie Pro lie Thr Asn Leu Thr Phe 290 295 300 lie Asn lie Thr Gly Thr Met Thr Gly Lys Lys Ser Thr Pro Val Leu 305 310 315 320 lie Val Cys Ala Glu Asp Ala Cys Arg Asp Trp Lys Trp Ser Asn Val 325 330 335 Ser lie Thr His Ala Ala Lys Pro Ser Lys Cys Ser Tyr lie Pro Glu 340 345 350 Gly Phe lie Cys <210> 33 <211> 376 <212> PRT <213> Chrysomela tremuiae <400> 33 Met Met Ser Phe Ser Glu Ser Val Thr Leu Leu Leu Leu Val Val Val 1 5 10 15 Ser lie Ser Ala Thr Leu Asn Gly Tyr Glu Ser lie Asp Tyr Ala Thr 25 30 Ala Val Asn Leu Thr Ala Asp Tyr Cys Tyr Ala Arg Ser Tyr Glu Glu 40 45 Val Ser Ser lie Val Lys Glu Cys Lys Tyr lie Val Leu Val Asp Phe 55 60 Glu Val Pro Ala Gly Lys Thr Leu Glu Leu Asp Leu Gin Lys Asn Ala 70 75 80 Val Leu Leu Phe Glu Gly Thr lie Lys Phe Ala Val Ala His Trp Asp 90 95 Gly Pro Phe lie His lie Thr Gly Lys Asn lie Gin Val lie Giy Giy 105 110 Thr Asn His Leu lie His Giy Gin Giy Glu Leu Tyr Trp Asp Giy His 120 125 Giy Giy Ser Giy Giy Val Lys Lys Pro Glu Lys Val Phe Ser Val Asn 135 140 Val Asn Giy Gly lie Phe Lys Asn lie Asn lie Lys Asn Cys Pro Thr 150 155 160 Ala Cys Vai Lys lie Tyr Giy Asn Asp Leu Thr The Ser Giy Phe Asn 170 175 lie Asp Leu Asn Asp Giy Phe Lys Asn Lys Leu Ala Lys His Thr Asp 185 190 Ala Met Thr Phe Ser His Ser Asn Asn lie Val lie Glu Asn Ser Lys 200 205 lie Val Asn Gin Asp Asp Cys Val Asn Vai lie Cys Ser Ser Asn Ala 210 215 220 Val lie Arq Asn Val His Cys Trp Giy Ser His Gly Leu Thr lie Ser 225 230 235 240 Ser Giy Leu Ser Thr Ser Asp Arg Asn Asn Asp Val Gin Asn lie Thr 245 250 255 Tyr Glu Asp Cys Ser lie Giy Giy Ser Leu Ser Giy lie His Phe Lys 260 265 270 Thr His Pro Ala Gly Gly His Gly Thr Val Asp Gin Val Thr Tyr Arg 275 280 285 Asn lie Glu lie Lys Asp Val Thr Arg His Gly lie Glu lie Gin Gin 290 295 300 Asp Tyr Pro Asn Asn Asp Gly His Pro Ser Asn Asn lie Lys lie Asn 305 310 315 320 Asn Leu Asn Leu lie Asn Val Thr Gly Thr Val Lys Gly Lys Thr Thr 325 330 335 Arg Gly Val Arg lie Ser Cys Gly Ser Gly Ser Cys Thr Asn Trp Lys 340 345 350 Trp Ser Asp Val Lys lie Thr Gly Ala His Lys Lys Asp Asp Cys Asn 355 360 365 Phe His Pro Asp Gly Tyr Ser Cys 370 375 <210> 34 <211> 365 <212> PRT <213> Gastrophysa viriduia <400> 34 Met Trp Phe lie Arg Leu lie Giy Val Leu Ser Ala Ala Ser lie Ala 1 5 10 15 Val Thr Ala Ser Pro Ala Gly Asp Gln Ser Cys Thr Ile Thr Asn Phe 25 30 Glu Gin Val Ser Ser Val lie Ala Asp Cys Thr Asp lie Arg Vai Ser 40 45 Asp Leu Gin Vai Pro Ala Giy Giu Thr Leu Asp Leu Giu Phe Lys Lys 55 60 Thr Gly Vai Thr lie Thr Phe Giu Gly Lys Thr Thr Phe Gly Tyr Lys 70 75 80 Glu Trp Ala Gly Pro Leu Leu Arg lie Lys Gly Lys Ala Leu Thr Val 90 95 Val Giy Ala Giu Gly Ser Val Leu Asp Gly Gin Giy Gin Leu Tyr Trp 105 110 Asp Giy Lys Giy Giy Lys Giy Lys Lys Lys Pro Lys Phe Phe Lys Tie 120 125 Lys Ala Thr Giu Giy Ser His Phe Lys Asn lie Asn Leu Leu Asn Cys 135 140 Pro Val Gin Cys Thr Ser lie Asp His Ser Gly Pro Leu Thr Leu Ser 150 155 160 Giy Trp Thr lie Asp Vai Ser Aia Giy Asp Lys Asp Asn Leu Giy His i65 170 175 Asn Thr Asp Gly Phe Asp lie Asn Thr Thr Asp Gly Leu Val lie Glu i85 190 Asn Thr lie Vai Lys Asn Gin Asp Asp Cys lie Ala Vai Asn Gin Giy 200 205 Thr Asn Phe Leu Phe Ser Asn Met Giu Cys Ser Giy Giy His Giy Met 210 215 220 Ser Leu Ser Vai Giy Thr Ser His Giu lie Ser Lys Asn Thr Vai Arg 225 230 235 240 Asn lie Thr Phe Aia Asp Ser Vai Vai Aia Lys Ser Arg Asn Giy lie 245 250 255 His lie Lys Thr His Thr Asn Ser Giy Gin Giy lie lie Aia Asp Vai 260 265 270 Thr Tyr Arq Asn lie Thr Met Thr Asp lie Trp Arg Tyr Aia Vai Asn 275 280 285 Vai Giu Gin Asp Tyr Lys Giu Giy Ser Pro Thr Giy His Pro Vai Giy 290 295 300 Asn lie Pro lie Giu Giy Leu Thr Leu Asp Asn Vai Arg Giy Thr Leu 305 310 315 320 Thr Giy Giy Giu Ser Vai Pro Vai Tyr lie lie Cys Aia Giu Giy Aia 325 330 335 Cys Thr Asp Phe Asn Trp Ser Ser Vai Thr Phe Asp Giy Aia Aia Asn 340 345 350 Pro Ser Asn Cys Ser Leu lie Pro His Giy Phe Pro Cys 355 360 365 <210> 35 <211> 369 <212> PRT <213> Gastrophysa viridula <400> 35 Met Phe Ser Leu Lys Val Leu Thr Ala Val Ala Ala Ala Phe Ala Ala 1 5 10 15 Val Ser Ala Leu Pro Thr Glu Ile Glu Thr Pro Val Gly Ser Gly Cys 25 30 Val lie Thr Asn Ile Asp Gln Val Ser Ser Val Thr Ser Ser Cys Thr 40 45 Asn I]e Va] Va] Ser Ser Leu Thr Va] Pro A]a G]y Lys Thr Leu Lys 55 60 Leu Gln Leu Lys Thr Gly Thr Thr Leu Thr Phe Lys Gly Lys Thr Val 70 75 80 Phe Glu Ala Thr Gln Trp Gln Gly Asp Leu Met Glu Ile Ser Gly Asp 90 95 Lys lie Thr Val Thr Gly Glu Ser Gly His Val Leu Asp Gly Gln Gly 105 110 Ala Lys Tyr Trp Asp Gly Lys Gly Asp Lys Gly Val Lys Lys Pro Lys 120 125 Phe Ile Arg Ile Lys Ala Thr Gly Gly Ser Val Phe Lys Gly Leu His 135 140 Val Leu Asn Cys Pro Lys Gln Cys Val Ser Leu Asn Ser Cys Asp His 150 155 160 Val Thr Ile Asp Asn Trp Thr Val Asp Val Ser Ala Gly Asp Lys Asn 170 175 Asn Leu Gly His Asn Thr Asp Gly Phe Asp Ile Ser Ser Ser Thr Asn 185 190 Leu Ile Ile Ser Asn Ser Val Val Lys Asn Gln Asp Asp Cys Val Ala 200 205 lie Asn Lys Ala Gin His Val Thr lie Ser Asn Leu Lys Cys Ser Gly 210 215 220 Gly His Gly Leu Ser Leu Ser Val Gly Met Ser Lys Thr Ser Val Ser 225 230 235 240 Asp Asn Thr Val Thr Asp Val Ala Phe Lys Asp Cys Thr Val Thr Asn 245 250 255 Ser Asp Asn Gly lie His lie Lys Thr His Ser Asp Giy Giy Ala Giy 260 265 270 Val lie Lys Asn Val Ser Tyr Lys Asn lie His Leu Ser Giy lie Arg 275 280 285 Lys Tyr Giy Val Asn lie Glu Gin Asp Tyr Glu Asn Giy Lys Pro Thr 290 295 300 Giy Thr Pro Lys Asn Asn lie Pro lie Thr Giy Leu Thr lie Ser Giy 305 310 315 320 Val Thr Giy Thr Met Thr Giy Ser Asn Ser Lys Ala Val Tyr lie Leu 325 330 335 Cys Ala Ser Gly Giy Cys Ser Gly Trp Thr Trp Gly Val Ser lie Thr 340 345 350 Giy Ser His Lys Asp Asn Tyr Cys Lys Asn Gin Pro Ser Asn Val Lys 355 360 365 Cys <210> 36 <211> 359 <212> PRT <213> Gastrophysa viridula <400> 36 Met Tyr Ser Leu Leu Leu lie Leu Leu Val Ser lie Pro Ser Leu Leu 1 5 10 15 Pro Arg Pro Val Ala Ala Ala Asp Cys Thr lie Thr Glu Phe Ser Gin 25 30 Val Ser Ser Val Val Ala Ser Cys Thr Asp lie Val lie Ser Glu Leu 40 45 Val Val Pro Gly Gly Glu lie Leu Glu Leu Asp Leu Leu Pro Gly Thr 55 60 Lys Val Thr Phe Glu Gly Thr Thr Lys Phe Glu Tyr Tyr Tyr Trp Ala 70 75 80 Gly Pro Leu lie Arg Val Arg Gly Glu Asn Val His Phe Lys Ala Ala 90 95 Pro Gly Ser Val Leu Asp Gly Gin Gly Ala Leu Trp Trp Asp Gly Lys 105 110 Gly Gly Ala Val Pro Gly Lys Pro Tyr Met lie Glu lie Asp Vai Thr 120 125 Gly Gly Leu Phe Glu Asp lie Phe Leu Leu Asn Cys Pro His His Cys 135 140 Val lie lie Ser Ser Thr Asp Leu lie Leu Asp Arg Trp Thr lie Asp 150 155 160 Val Ser Gin Gly Asn Val Gly Glu Leu Gly His Asn Thr Asp Gly Phe 170 175 Asp Val lie Tyr Gly His Asn Val Thr lie Arg Asn Ser Thr Vai Ser 185 190 Asn Gin Asp Asp Cys Val Ala lie Asn Arg Gly Lys Gly Leu Thr lie 200 205 Thr Gin Met Asp Cys Thr Gly Ser His Gly Leu Ser lie Ser Val Gly 210 215 220 Phe Ser Lys His Ser Phe Leu His Asn Ser Val Val Asp Val Val Val 225 230 235 240 Glu Asp Ser Val Leu Arq Asp Gly Ala Asn Gly lie His Val Lys Thr 245 250 255 His Thr Asp Gly Tyr Ala Giy Giu lie Arq Asn Vai Vai Tyr Arq Asn 260 265 270 Ile His Met Thr Gly Ile Glu Arg Tyr Gly lie Gin lie Gin Gin Asp 275 280 285 Tyr Pro Ser Gly Asn Gly Arg Pro Val Gly Asn lie Pro lie Thr Asn 290 295 300 Leu Thr Phe Ser Asn lie Thr Gly Thr Met Thr Gly Arg Lys Ser Thr 305 310 315 320 Pro Val Ala lie Thr Cys Ala Glu His Ala Cys Ala Asn Trp Asn Trp 325 330 335 Ser Gly Val Phe lie Thr Gly Ala Ala Lys Pro Ser Asn Cys Thr Tyr 340 345 350 Val Pro Glu Gly Phe Asp Cys <210> 37 <211> 368 <212> PRT <213> Gastrophysa viridula <400> 37 Met His Cys Thr Ala lie Vai Leu lie Phe Giy Ala Leu Val Ala Leu 1 5 10 15 Val Gly Ser Ala Pro Thr His Asn Cys Thr lie Arg Lys Phe Ser Glu 25 30 Val Gin Ser Val lie Asn Gly Cys Thr Asp lie Leu Val Ser Asn Leu 40 45 Thr Met Pro Aia Gly lie Val Leu Glu Leu Asp Leu Leu Asn Gly Thr 55 60 Thr Met Thr Phe Glu Gly Thr Thr Leu Phe Glu Tyr Thr Pro Trp Ser 70 75 80 Gly Pro Leu lie Arg Val Lys Gly Val Asp Val Thr Leu Gin Gly Gin 90 95 Pro Giy Ser Vai Phe Asp Giy Gin Giy Pro Leu Tyr Trp Asp Giy Lys 105 110 Giy Asp Lys Giy Pro Lys Lys Pro Gin Phe Met Lys lie Giu Vai Phe 120 i25 Giy Arg Ser lie Phe Lys Asn lie Lys Leu Leu Asn Cys Pro His His i35 i40 Cys Vai Tyr lie Giy Lys Ser Asp Giy Leu Thr lie Thr Asn Trp Thr i45 i50 i55 i60 lie Asp Asn Ser Aia Giy Asp Leu His Asn Phe Thr Giy His Asn Thr i65 170 i75 Asp Giy Phe Asp lie Ser Aia Aia Lys Asn Leu lie lie Giu Asn Thr i80 i85 190 Aia Vai lie Asn Gin Asp Asp Cys lie Aia lie Arg Tyr Giy Ser Asn i95 200 205 lie Leu Vai Arg Asn Met Tyr Cys Ser Giy Giy His Giy Leu Ser Leu 2i0 2i5 220 Ser Vai Giy Phe Asn Lys Thr Ser Tyr Pro Giu Asn Vai Vai Giu Asn 225 230 235 240 Vai Vai lie Giu Asp Ser Leu Leu Vai Asn Ser Aia Asn Giy lie His 245 250 255 Vai Lys Thr His Thr Asp Giy Tyr Phe Giy Leu lie Lys Asn Vai Thr 260 265 270 Tyr Arg Asn lie Giu Met Arg Giy lie Thr Asn Tyr Giy lie Asn Vai 275 280 285 Gin Gin Asp Tyr Cys Asp Giy Arg Giy Thr Giy Thr Aia Lys Ser Asn 290 295 300 Vai Pro lie Asp Ser Leu Asn lie lie Asn Vai Thr Giy Thr Aia Aia 305 310 315 320 Lys Gly Ser Lys Leu Thr Gly Val Tyr lie Phe Cys Ala Glu Gly Ser 325 330 335 Cys Arg Asn Trp Thr Trp Asp Asp Val Lys lie Thr Gly Ala Lys lie 340 345 350 Gly Asp Phe Cys Thr Phe lie Pro Ser Gly Tyr Glu Cys His Lys Val 355 360 365 <210> 38 <211> 374 <212> PRT <213> Gastrophysa viridula <400> 38 Met Thr Ser His Leu lie Pro Leu Leu Ser lie Leu Val Phe Ser Val 1 5 10 15 Ala Val Thr Ala Thr Ser Thr Asp Ser Tyr Glu His lie Asp Tyr Glu 25 30 Lys Ala Lys Asn Leu Thr Ala Asp Tyr Cys Tyr Ala Arg Ser Tyr Ala 40 45 Glu Val Ala Asn Val Val Glu Lys Cys Arg Tyr lie Val Leu Val Asn 55 60 Phe Glu Val Pro Ala Gly Lys Thr Leu Gin Leu Asn Leu Gin Lys Asn 70 75 80 Ser Val Leu Leu lie Glu Gly Thr lie Thr Phe Ala Val Ala His Trp 90 95 Asp Gly Pro Leu Met Ser lie Asn Gly Lys Asn Leu Gin Val Leu Gly 105 110 Gly Thr Asn His Leu lie His Gly Gin Gly Glu Lys Tyr Trp Asp Gly 120 125 Arg Gly Gly Gly Gly Val Gin Lys Pro Gin Arq Val Met Ser lie Asn 135 140 Val Gin Gly Gly Ser Phe Lys Asn lie Asn lie Lys Asn Cys Pro Met 150 155 160 Phe Cys Val Ser lie lie Gly Ser Asp Leu Thr Phe Ser Gly Phe Asn 170 175 lie Asp Asn Asn Asp Gly Phe Lys Gly Gly Leu Ala Val Asn Thr Asp 185 190 Ala Met Gly Phe Val Tyr Ser Asn Asn lie Leu lie Glu Asn Ser Lys 200 205 lie lie Asn Gin Asp Asp Cys lie Asn Val Met Ala Ser Ser Asn Ala 210 215 220 Val lie Arq Asn Val His Cys Trp Gly Ser His Gly Leu Ser Phe Ser 225 230 235 240 Ser Gly Leu Ser Lys Thr Asp Glu Asn Asn Asp lie His Asn Val Thr 245 250 255 Phe Gin Asp Cys Ser lie Gly Gly Ser Leu Asn Gly lie His lie Leu 260 265 270 Thr Met Pro Ser Gly Gly Lys Gly Lys lie Ser Gin Val Thr Tyr Arg 275 280 285 Asn lie Gin lie lie Asp Val Thr Gin Arg Ala lie Glu Phe Arq Gin 290 295 300 Asp Tyr Leu Asp Asn Gly His Pro Lys Asn Asn lie Val lie Asp Gly 305 310 315 320 Leu Thr Leu Asp Asn lie Tyr Gly Thr Val Thr Gly Asn Ala Val Ala 325 330 335 Val Tyr lie Asn Cys Gly Ser Gly Ser Cys Lys Asn Trp Asn Trp Ser 340 345 350 Gly Val Lys lie Thr Gly Ala Lys Thr Lys Asp Tyr Cys Asn Phe Val 355 360 365 Pro His Gly Tyr Ser Cys <210> 39 <211> 372 <212> PRT <213> Gastrophysa viridula <400> 39 Met Phe Met Lys Tyr Phe Val Thr Phe Ser Val lie Val Val Gly lie 1 5 10 15 Ser Ala Thr Thr lie Glu Tyr Asp Tyr Glu Thr Ala Lys Asn Leu Thr 25 30 Ser Asp Tyr Cys Tyr lie Lys Ser Tyr Asn Glu Ala Pro Ala lie Val 40 45 Lys Lys Cys Thr His lie Val Val Thr Gly Phe Glu Val Pro Ala Gly 55 60 Lys Thr Leu Giu Leu Asn Leu Leu Lys Gin Ser Vai Leu Leu Phe Giu 70 75 80 Gly Thr Leu Thr Phe Gly Val Ala His Trp Asp Giy Pro Phe lie His 90 95 lie Thr Gly Gin Asn lie Gin Val lie Gly Gly Thr Asn His Val Tie 105 110 His Gly His Gly Glu Lys Tyr Trp Asp Gly Gin Gly Asp Ser Gly Gly 120 125 Vai Lys Lys Pro Arg Arg Val Leu Ser Val Gin Vai Gin Lys Ala lie 135 140 Phe Lys Asn lie Asn lie Lys Asn Cys Pro Leu Phe Cys Ala Thr Ala 150 155 160 Leu Gly His Asp lie Thr Phe Thr Gly Phe Asn lie Asp Asn Asn Asp 170 175 Gly Phe Lys Asn Gly Val Ala Arg Asn Thr Asp Gly lie Ser Phe Ala 185 190 Gin Cys Ser Asn lie lie Val Lys Asp Ser Lys Val Tyr Asn Gin Asp 200 205 Asp Cys Val Asn lie Leu Gly Gly Cys Lys Asn Val Tyr Val Glu Asn 210 215 220 Val His Cys Trp Gly Ser His Gly Leu Ser lie Thr Ser Gly Met Ala 225 230 235 240 Thr Thr Ala Asp Lys Asn Asp lie Tyr Asn Val Thr Tyr Gin Asp Cys 245 250 255 Ser lie Giy Giy Ser Tyr Ala Giy lie His Val Lys Thr Thr Gin Pro 260 265 270 Cys Leu Gly Pro Gly Thr lie Arg Lys lie Thr Tyr Lys Asn lie Gin 275 280 285 lie Thr Asp Vai lie Arg Asp Ala lie Giu lie Gin Gin Asp Tyr Pro 290 295 300 Lys Asn Asp Gly Asn Ser Ala Asn Lys Met Ala Met Thr Glu Val Asn 305 310 315 320 Leu lie Asp Val Thr Gly Ser Val Thr Gly Ser Ala Arg Arg Val Arg 325 330 335 lie Asn Cys Gly Ser Gly Ser Cys Ser Asn Phe Arg Trp Ser Asn Val 340 345 350 Lys lie Thr Gly Ser Lys Thr Asp Asp Ser Cys Asn Tyr lie Pro Ser 355 360 365 Gly Tyr Ser Cys <210> 40 <211> 374 <212> PRT <213> Gastrophysa viridula <400> 40 Met Tyr Met Cys lie Lys Ser Ser lie Leu Phe Ser lie Val lie Val 1 5 10 15 Gly lie Ser Ala Glu Thr lie Asp Tyr Asp Tyr Glu Thr Ala Lys Asn 25 30 Leu Thr Ser Asp Tyr Cys Tyr lie Lys Ser Tyr Asn Glu Ala Pro Ala 40 45 lie Val Lys Lys Cys Thr His lie Val Val Thr Gly Phe Glu Val Pro 55 60 Ala Gly Lys Thr Leu Glu Leu Asn Leu Leu Gin His Ser Val Leu Leu 70 75 80 Phe Glu Gly Thr Leu Thr Phe Gly Val Ala His Trp Ala Gly Pro Phe 90 95 lie Tyr lie Thr Gly Gin Asn lie Gin Val lie Gly Gly Thr Asn His 105 110 Leu lie His Gly Gly Gly Glu Lys Tyr Trp Asp Gly Leu Gly Gly Ser 120 125 Gly Val Lys Lys Pro Glu Arg Val Phe Phe Met Asn Ala Gin Val Ala 135 140 Thr Val Lys Asn lie Asn lie Lys Asn Cys Pro Leu Phe Cys Val Ser 150 155 160 Val Leu Gly His Asp Leu Thr Phe Ser Gly lie Asn lie Asp Asn Thr 170 175 Asp Gly Phe Ala Asn Gly Leu Ala Arg Asn Thr Asp Gly lie Thr Phe 185 190 Ala Gin Cys Lys Asn Val Val Leu Glu Asn Ser Lys lie lie Asn Gin 200 205 Asp Asp Gly Val Asn lie Leu Gly Gly Cys Ser Asn Val Tyr Val Arg 210 215 220 Asn Val His Cys Trp Gly Ser His Gly Leu Ser Val Thr Ser Gly Met 225 230 235 240 Ala Thr Thr Ala Ala Lys Asn Asp Val Tyr Asn Val Thr Phe Glu Asp 245 250 255 Cys Ser Asn Gly Gly Ser Phe Val Gly lie His Val Lys Thr Thr Gin 260 265 270 Pro Cys Leu Giy Pro Giy Ser Phe Lys Gin lie Thr Tyr Lys Asn lie 275 280 285 Gin lie Ser Giu Vai Thr Arg Ser Aia lie Giu lie Gin Gin Asp Tyr 290 295 300 Pro Lys Asn Asp Giy Asn Ser Aia Asn Thr Met Lys lie lie Giy Vai 305 3i0 3i5 320 Asp Leu lie Asn Vai Thr Giy Ser Vai Lys Thr Thr Ser Aia Arq Lys 325 330 335 Vai Arq lie Asn Cys Giy Ser Giy Ser Cys Ser Asn Phe Lys Trp Ser 340 345 350 Asn Vai Lys lie Thr Giy Ser Thr Thr Aia Asp Ser Cys Asn Tyr His 355 360 365 Pro Ser Giy Tyr Ser Cys <2i0> 4i <2ii> 366 <2i2> PRT <2i3> Leptinotarsa decemiineata <400> 4i Met Cys Asp Thr Leu Lys Phe Phe Giy lie Leu Giy Phe Leu Vai Leu i 5 10 15 Thr Ser Aia Gin Pro Ser Leu Asn Gin Ser Aia Ser Cys Thr lie Ser 25 30 Lys Phe Ser Asp Vai Ser Aia Vai Leu Ser Arg Cys Thr Asp lie Vai 40 45 Vai Ser Asn Leu Thr Vai Pro Giy Giy Lys lie Leu Asp Leu Asp Leu 55 60 Gin Ser Giy Ser Thr Vai Thr Phe Giu Giy Thr Thr Lys Phe Giu His 70 75 80 Ala Leu Trp Ala Gly Pro Leu Val Arg Val Lys Gly Glu Lys Val Leu 90 95 Val Gln Gly Ala Thr Gly Ser Val Leu Asp Gly Gln Gly Ala Leu Trp 105 110 Trp Asp Gly Lys Gly Gly Ser Val Ala Gly Lys Pro Tyr Phe Phe Glu 120 125 Ile Asp Val Ser Gly Gly Ser Leu Phe Lys Asn Val Phe Leu Leu Asn 135 140 Cys Pro His His Cys Val Ile Ile Ala Ser Ser Asp Thr Thr Leu Thr 150 155 160 Gly Trp Thr Ile Asp Asn Ser Asp Gly Asp Lys His Gln Leu Gly His 170 175 Asn Thr Asp Gly Phe Asp Ile Ile Asn Gly Gln Asn Ile Val Ile Thr 185 190 Asn Ser Thr Val Lys Asn Gln Asp Asp Cys Val Ala Ile Asn Arg Gly 200 205 Ser Asn Met Leu Ile Ser Asn Leu His Cys Ser Gly Ser His Gly Leu 210 215 220 Ser Leu Ser Val Gly Phe Ser Lys His Ser Phe Ser His Asn Lys Val 225 230 235 240 Thr Asn Val Thr Phe Arg Asp Ser Val Ile Val Asn Ala Gly Ser Gly 245 250 255 Ile His Val Lys Thr His Thr Asp Gly Gly Leu Gly Glu Ile Arg Asn 260 265 270 Val Thr Tyr Glu Asn Ile Tyr Met Ala Gly Ile Thr Asn Ser Gly Ile 275 280 285 Asn Ile Gln Gln Asp Tyr Ala Asn Gly Gly Ser Thr Gly Ile Leu Asn 290 295 300 Asn Asn Ile Pro Ile Thr Ser Leu Thr Leu Thr Asn Val Thr Gly His 305 310 315 320 Val Val Gly Lys Lys Ala Leu Arg Val Lys Ile Tyr Cys Ala Asn Glu 325 330 335 Gly Cys Phe Asp Trp Lys Trp Ser Gly Val Glu Val Leu Gly Gly Arg 340 345 350 Gln Lys Asn Gln Cys Thr Tyr Glu Pro Glu Gly Phe Ser Cys 355 360 365 <210> 42 <211> 361 <212> PRT <213> Leptinotarsa decemlineata <400> 42 Met Phe Ser Val Lys Val Ile Ser Leu Leu Val Thr Cys Trp Ala Leu 1 5 10 15 Phe Ser Ile Thr Asp Ala Ser Ala Val Gly Glu Asp Cys Thr Ile Thr 25 30 Asn Phe Ser Gln Val Ser Ser Val Val Gln Arg Cys Asn Asn Ile Val 40 45 Val Arg Ser Leu Asn Val Pro Ala Lys Gln Arq Leu His Leu Asn Leu 55 60 Lys Arq Gly Ser Ser Leu Thr Phe Ser Gly Ile Thr Arg Phe Gly Val 70 75 80 Ala Glu Trp Asp Gly Pro Leu Val Ile Ile Gln Gly Asp Asn Leu Lys 90 95 Val Asn Gly Glu Leu Gly Ser Lys Leu Asp Gly Gln Gly Ala Glu Tyr 105 110 Trp Asp Gly Lys Gly Gly Ser Gly Ser Lys Lys Pro Val Leu Ile Gln 120 125 Ile Ile Gly Ser Gly Asp Phe Asn Asn Ile His Leu Leu Asn Cys Pro 135 140 Glu Arg Cys Ala Ser Val Leu Gly Ser His Leu Ser Leu Val Gly Trp 150 155 160 Asn lie Asp Val Ser Ala Gly Asp Lys Asn Asn Leu Gly His Asn Thr 170 175 Asp Gly Phe Asp Val Val Gly Thr Asp lie Thr lie Arg Asn Ser Val 185 190 Val Lys Asn Gin Asp Asp Cys Leu Val Val Asn Arg Gly Her Asp Met 200 205 His Phe Gin Asn lie Tyr Cys Ser Giy Giy His Giy Leu Ser Leu Ser 210 215 220 lie Giy Met Ser Lys Thr Ser Tyr Asn Asp Asn Val Ala Arq Asn lie 225 230 235 240 Thr Phe Lys Asp Cys Thr Val Val Asn Ser Asp Asn Ala lie His lie 245 250 255 Lys Thr His Lys Asp Giy Ala Gin Giy Leu lie Gin Asp Val Thr Tyr 260 265 270 Lys Asn lie Thr Leu Tyr Asn lie Arg Asn Tyr Giy Met Gin lie Gin 275 280 285 Gin Asp Tyr Pro Asn His Asp Gly Arg Pro Val Gly Asn Val Pro lie 290 295 300 Arg Gly Leu lie lie Thr Asp Phe Lys Gly Thr Met Thr Gly Lys Asn 305 310 315 320 Ser Val Pro Val Glu Val Val Cys Ala Ala Gly Ala Cys Ser Asp Trp 325 330 335 lie Trp Ser Glu lie Lys lie Ser Gly Asn Ser Lys Pro Cys Ser Met 340 345 350 Asn Tyr Tyr Pro Ser Gly Phe Ser Cys 355 360 <210> 43 <211> 365 <212> PRT <213> Leptinotarsa decemlineata <400> 43 Met lie Phe Leu Thr Glu lie lie Phe Val Thr Thr Leu Leu Ser Gly 1 5 10 15 Ser Phe Ser Leu Ala Ser Ser Pro Giy Cys Thr lie Arg Arg Tyr Ser 25 30 Asp Vai Pro Ser Vai Leu Ser Asn Cys Thr Thr lie lie lie Asp Asn 40 45 Leu Vai Phe Pro Ala Giy lie Thr Phe Giu Leu Tyr Leu Gin Lys Gly 55 60 Thr Thr Va] Thr Phe Lys G]y Thr Thr Arg Phe G]u Tyr Thr Pro Trp 70 75 80 Ser Gly Pro Leu lie Arg Val Lys Gly Ser Asn lie Thr lie Gin Gly 90 95 Glu Pro Gly Ser Val Leu Asp Gly Gin Gly Pro Leu Tyr Trp Asp Gly 105 110 Lys Gly Asp Lys Gly Pro Lys Lys Pro Gin Phe lie Lys lie Glu Ala 120 125 Asn Glu Arq Ser Val Met Lys Asn lie Asn lie Leu Asn Cys Pro His 135 140 His Cys lie Tyr Val Gly Lys Ser Asp Gly Leu Thr lie Ser Asp Trp 150 155 160 Val lie Asp Val Ser Asp Gly Asp Lys Asn Asn Phe Thr Gly His Asn 170 175 Thr Asp Gly Phe Asp Val Ser Giy Ala Asn Asn Leu lie lie Glu Asn 185 190 Thr Arg Val lie Asn Gin Asp Asp Cys lie Ala lie Arg Tyr Gly Thr 200 205 Asn lie Thr Val Arg Asn Met Tyr Cys Ser Gly Gly His Gly Leu Ser 210 215 220 Leu Ser Val Gly Phe Asn Lys Thr Ser Tyr Pro Glu Asn Val Val Ser 225 230 235 240 Asn Val Leu lie Glu Asp Ser Thr Val Val Arg Ser Ala Asn Ala lie 245 250 255 His lie Lys Thr His Thr Asp Gly Trp Phe Gly Leu lie Asn Asn lie 260 265 270 Thr Tyr Arg Asn lie Glu Leu lie Gly lie Thr Asn Phe Gly lie Asn 275 280 285 lie Gin Gin Asp Tyr Ala Asn Gly Glu Gly Thr Gly Thr Val Arq Asp 290 295 300 Asn lie Pro lie Arg Asn lie Asn Leu Ser Asn lie His Gly Asn Met 305 310 315 320 Lys Gly Arg Lys Thr Arg Ala Phe Tyr lie Tyr Cys Ala Lys Asp Ala 325 330 335 Cys Ser Asp Trp Lys Trp Val Asp Met Lys lie Glu Asn Ala Ser Lys 340 345 350 Ser Asn Ser Cys Asn Tyr Ser Pro Pro Asp Tyr Pro Cys 355 360 365 <210> 44 <211> 361 <212> PRT <213> Leptinotarsa decemlineata <400> 44 Met Phe Phe Ala Lys Val lie Ser lie Leu Ser lie His Ser Ala lie 1 5 10 15 Ser lie Thr Ala Trp Gly Ser Ala lie Ser Gly Giy Cys Thr lie Thr 25 30 Giu Phe Ser Gin Val Ser Gin Vai Val Asn Ser Cys Thr Asn lie Vai 40 45 Val Lys Asn Leu Asn Val Pro Ala Gin Gin Thr Leu His Met Lys Leu 55 60 Lys Lys Glu Ser Thr Leu Ser Phe Glu Gly Thr Thr lie Phe Gly Val 70 75 80 Ala Asn Trp Glu Gly Pro Leu lie Lys lie Glu Gly Asp Thr Val Lys 90 95 lie Asp Gly Ala Glu Gly Ser Lys Phe Asp Gly Gin Gly Ala Glu Tyr 105 110 Trp Asp Gly Lys Gly Gly Gin Gly Ser Lys Lys Pro Leu Phe Phe Thr 120 125 Phe Gin Gly Ser Gly Glu Leu Thr Asn lie Asn Leu Leu Asn Cys Pro 135 140 Glu Arg Cys Ser Asp Val His Gly Ser His lie Thr lie Asp His Trp 150 155 160 Asn lie Asp Val Ser Ala Gly Asp Lys Asn Asn Leu Gly His Asn Thr 170 175 Asp Gly lie Gly Val Trp Gly Glu Asn lie lie Val Lys Asn Ser Val 185 190 Val Arg Asn Gin Asp Asp Cys Phe Val Met Ala Arg Gly Arg Asp Tie 200 205 His Val Asn Asn Leu Tyr Cys Ser Giy Gly His Giy lie Ser Met Ser 210 215 220 Val Gly Leu Ser Lys Thr Ser Phe Glu Ser Asn Phe Val Gin Asn Val 225 230 235 240 Thr Phe Lys Asp lie Thr Leu Val Asp Ser Asp Asn Gly lie His Leu 245 250 255 Lys Thr His Lys Asp Gly Gly Pro Gly Leu Val Glu His lie Thr Tyr 260 265 270 Lys Asn lie Thr Leu Lys Arq lie Arg Arq Tyr Gly lie Glu lie Gin 275 280 285 Gin Asp Tyr Pro Giu Asn Asp Giy Lys Pro Thr Asp Asn lie Pro lie 290 295 300 Arg Asp Vai Thr Vai lie Giy Vai Arg Giy Thr Met Thr Giy Lys His 305 310 315 320 Ser Vai Pro Vai Arg Vai Ser Cys Ser Thr Giy Aia Cys Aia Asn Trp 325 330 335 Asn Trp Ser Giu Vai Asp lie Lys Giy Asn His Lys Pro Cys Ser Met 340 345 350 Asn Phe Lys Pro Asn Giy Phe Ser Cys 355 360 <2i0> 45 <2ii> 36i <2i2> PRT <2i3> Leptinotarsa decemiineata <400> 45 Met Phe Phe Aia Arg Vai lie Asn Leu Leu Vai Thr Giy Trp Aia Leu i 5 10 i5 Phe Ser lie Thr Asn Aia Ser Vai lie Giy Giu Asp Cys Thr lie Thr 25 30 Asn Phe Ser Giu Vai Ser Ser Vai Vai Gin Arq Cys Asn Asn lie Vai 40 45 Vai Arg Ser Leu Asn Vai Pro Aia Lys Lys Ser Leu His Leu Ser Leu 55 60 Lys Lys Giy Ser Ser Leu Thr Phe Ser Giy lie Thr Thr Phe Giy Vai 70 75 80 Aia Giu Trp Giy Giy Pro Leu Vai Vai lie Gin Giy Asp Asn Leu Arg 90 95 Vai Asn Giy Giu Leu Giy Ser Lys Leu Asp Giy Gin Giy Aia Lys Tyr 105 110 Trp Asp Giy Lys Giy Giy Lys Giy Ser Thr Lys Pro Vai Leu lie Gin 120 125 lie Lys Gly Ser Gly Asp Phe Asn Asn lie His Leu Leu Asn Cys Pro 135 140 Glu Arg Cys Ala Ser Val His Giy Ser His Leu Ser Leu Val Giy Trp 150 155 160 Asn lie Asp Val Ser Ala Giy Asp Lys Asn Lys Leu Giy His Asn Thr 170 175 Asp Giy Phe Asp Val Ser Giy Thr Asp lie Thr Val Arg Asn Ser Val 185 190 Val Lys Asn Gin Asp Asp Cys Leu Val Vai Asn His Giy Asn Asn Met 200 205 His Phe Gin Asn Leu Tyr Cys Ser Giy Giy His Giy Leu Ser Leu Ser 210 215 220 lie Giy Met Ser Lys Thr Ser Tyr Asn Asp Asn Val Ala Arg Asn lie 225 230 235 240 Thr Phe Lys Asp Cys Thr Val Val Asn Ser Asp Asn Giy lie His lie 245 250 255 Lys Thr His Lys Asp Giy Thr Glu Giy Leu lie Gin Asp Val Thr Tyr 260 265 270 Lys Asn lie Thr Leu His Asp lie Arg Asn Tyr Giy Leu Glu lie Gin 275 280 285 Gin Asp Tyr Pro Asn His Asp Gly His Pro Val Gly Asn Val Pro lie 290 295 300 Arg Gly Leu Thr lie Thr Asp Val Lys Gly Thr Met Thr Gly Lys Lys 305 310 315 320 Ser Val Pro Val Glu Val Val Cys Ala Ala Gly Ala Cys Ser Asp Trp 325 330 335 lie Trp Ser Glu lie Lys lie Ser Gly Asn Lys Lys Pro Cys Ser Met 340 345 350 Asn Tyr His Pro Ser Gly Phe Phe Cys 355 360 <210> 46 <211> 355 <212> PRT <213> Leptinotarsa decemlineata <400> 46 Met Tyr Ala Leu Val Val Phe Thr Thr Leu Val Ala Ser lie Leu Ser 1 5 10 15 Phe Thr Ser Ser Lys Asn Cys Thr lie His Asn Phe Glu Glu Val Ser 25 30 Ser Val Leu Gin His Cys Ser Giu lie Thr Vai Phe Asn Vai Thr Vai 40 45 Pro Ala Giy Asn Thr Leu Giu Phe Asn Leu Lys Lys Asn Ser Vai Vai 55 60 Thr Leu Giu Giy Thr lie Thr Phe Gin Phe Gin Ala Trp Lys Giy Pro 70 75 80 Leu Leu Giu Phe Lys Giy Asp Giy lie Thr Vai Lys Giy Thr Lys Giy 90 95 Ser Vai lie Asp Giy Lys Giy Giu Leu Tyr Trp Asp His Leu Giy Asp 105 110 Lys Giy Leu Thr Lys Pro Gin Leu Vai Asn lie Vai Ala Ser Asn Asp 120 125 Ser Vai Phe Gin Asn Leu His Leu Arg Asn Cys Pro Asn His Cys Phe 135 140 Tyr Leu Arg Asn Ser Asp Asn Vai Thr lie Ser Giu lie Asp lie Asp 150 155 160 Vai Ser Asp Giy Asn Lys Asn Asn Phe Thr Giy His Asn Thr Asp Giy 170 175 Phe Asn Leu Giy Phe Vai Thr Asn Thr lie lie Lys Asn Cys Thr Vai 185 190 Lys Asn Gin Asp Asp Cys Vai Ser lie Asn Aia Aia Ser Asn lie Ser i95 200 205 lie Arg Asn Met Asp Cys Ser Giy Giy His Giy Leu Ser lie Ser Vai 2i0 2i5 220 Thr Arg Asn Pro Vai Asp Asn Vai Thr Tyr Ser Asp Ser lie Vai Arg 225 230 235 240 Asn Ser Aia Asn Giy Leu His Vai Lys Thr His Thr Asn Giy Giy Giu 245 250 255 Thr Vai lie Ser Asn lie Thr Tyr Lys Asn lie Lys Leu Giu Giy lie 260 265 270 Thr Asn Phe Giy lie Asn lie Gin Giu Asp Tyr Lys Asp Giy Giu Giy 275 280 285 Thr Giy lie Pro Aia Asn Asn lie Lys lie Vai Asn Leu lie lie Ser 290 295 300 Asn lie Thr Giy Thr Met Asn Giy Lys Asp Ser Lys Pro Vai Gin lie 305 3i0 3i5 320 lie Cys Giy Asp Thr Giy Cys Aia Asn Trp Gin Trp Ser Asp lie Tyr 325 330 335 lie Vai Giy Asn Ser Thr Pro Ser Tyr Cys Asn Tyr Aia Pro Ser Giy 340 345 350 Phe Asn Cys <2i0> 47 <2ii> 360 <2i2> PRT <2i3> Leptinotarsa decemiineata <400> 47 Met Leu Vai Aia Asp Leu lie Ser lie Leu lie Ser Phe Trp Giy Vai i 5 10 15 Ser Ser Met Vai Vai Thr Pro Vai Aia Giy Ser Ser Cys Thr lie Thr 25 30 Asp Tyr Ser Glu Val Ser Lys Val Val Ser Ser Cys Thr Lys lie Val 40 45 Leu Lys Ser Phe Glu Val Pro Ala Gly Lys Thr Leu Asn Leu Asn Leu 55 60 Gin Pro Glu Thr Phe Leu lie lie Asp Giy Thr lie Lys Phe Giy Lys 70 75 80 Ser Asn Trp Ala Giy Pro Leu Val Lys lie Gin Giy Arg Arg lie Lys 90 95 Val Ser Gly Thr Pro Gly Ser Val Phe Asp Gly Gin Gly Ala Glu Tyr 105 110 Trp Asp Gly Lys Gly Ser Gin Gly Thr Lys Lys Pro Val Leu Leu Arg 120 125 Val Asn Ala His Glu Ser Glu Phe Tyr Asn lie Asn Leu Leu Asn Cys 135 140 Pro Leu Arg Cys Ala Ser lie Gly Gly Ser Asp lie Phe Leu Asn Gly 150 155 160 Trp Thr lie Asp Val Ser Glu Ala Gly Asn Leu Gly Val Asn Thr Asp 170 175 Gly Phe Asp Val Ser Gly His Asn Met Thr lie lie Asn Ser Arg Val 185 190 Phe Asn Gin Asp Asp Cys Val Val Val Asn Arg Gly Ser Asp Met Phe 200 205 Phe Gin Asn lie Tyr Cys Lys Gly Gly His Gly Leu Ser Val Ala Val 210 215 220 Gly lie Asp Pro Thr Val Tyr Glu Asn Asn Val Val Ser Asn lie Thr 225 230 235 240 Tyr Gin Asp Cys lie Val Glu Asn Ser lie Asn Gly lie His Val Lys 245 250 255 Thr Val Lys Gly Gly Gly Pro Gly Leu lie Ser Asn Val Asn Tyr Asn 260 265 270 Arg lie Arg Met Met Asn lie Lys Glu Phe Ala lie Arg lie His Gin 275 280 285 Asp Tyr Pro Ser Asn Asp Lys Lys Pro Vai Giy Asn Val Pro lie Asp 290 295 300 Asp Leu Lys lie Ser His Val Glu Giy Asn Met Lys Giy Pro Arg Ser 305 310 315 320 Ser Val Val Glu Val Leu Cys Ala Ser Giy Ala Cys Ser Asn Trp Lys 325 330 335 Trp Ser Asp lie Ser Vai Thr Giy Asn Lys Leu Pro Cys Ser Met Asn 340 345 350 Tyr Lys Pro Ser Giy Phe Ser Cys 355 360 <210> 48 <211> 362 <212> PRT <213> Leptinotarsa decemlineata <400> 48 Met Tyr Phe Ala Ser Leu Val Ala lie Leu Ala lie Cys Cys Gly Leu 1 5 10 15 Phe Tyr Thr Val Asp Thr Asn lie Leu Lys Arg Asp Cys Asp lie Thr 25 30 Asp Phe Ser Gin Val Pro Asp Val Val Ser Arg Cys Thr Asn lie Arg 40 45 Val Trp Lys Met Asp Val Pro Ala Gly Glu Thr Leu Asn Leu Lys Leu 55 60 Gin Thr Gly Thr Thr Leu Tyr Phe Lys Asp lie Val Lys Phe Gly Val 70 75 80 Ala Asn Trp Asp Gly Pro Leu Val Asn lie Glu Gly Asn Asp Leu Arg 90 95 lie Tyr Gly Ala Ala Gly lie Giy Phe Ser Gly Gin Giy Glu Glu Tyr 105 110 Trp Asp Gly Lys Gly Ser Gin Gly Ser Lys Lys Pro Leu Phe Leu Lys 120 125 lie Asn Val Gin Gly Gly Glu Phe Tyr Val Leu Asn Leu Val Asn Cys 135 140 Pro Glu Arg Cys Thr Ser lie Thr Gly Ser Asp lie Leu lie Asn Gly 150 155 160 Trp Thr lie Asn Thr Ser Asp Gly Asp Lys Asp Asn Leu Giy Val Asp 170 175 Thr Vai Gly Phe Glu Vai Ser Gly Asp Asn lie Thr lie Arg Asn Ser 185 190 Leu Vai Phe Asn Gin Asp Asp Cys lie Arg Val Glu Arg Giy Ser Asn 200 205 Met Leu lie Ser Asn Asn Tyr Cys Asn Giy Giy Asn Gly Leu Asn Val 210 215 220 Giu Vai Giy Ala Asp Pro Giu Ala Tyr Giu Giu Asn Vai Vai Ser Asn 225 230 235 240 lie Thr Phe Asp Tyr Giy Val lie Glu Asn Ser Thr Tyr Giy lie His 245 250 255 Vai Lys Thr Val Lys Giy Ser Gly Pro Giy Leu Leu Gin Asn Val Ser 260 265 270 Phe Ser Ser lie Asn Met lie Giy lie Lys Asn Tyr Ser lie Arg lie 275 280 285 His Gin Asp Tyr Pro Ala Asp Asp Asn Lys Pro Glu Giy Asn Vai Pro 290 295 300 lie Arg Val Leu Asn Leu Lys Tyr Vai Thr Gly Asn Met Ser Gly Ser 305 310 315 320 Ser Ser Val Pro Vai Asp Val Val Cys Ala Ser Gly Ala Cys Ser Asp 325 330 335 Trp Thr Trp Ser Asn lie Ser lie Lys Gly Asn Ser Thr Pro Cys Ser 340 345 350 Met Asn Tyr Glu Pro Ser Gly Phe Phe Cys 355 360 <210> 49 <211> 372 <212> PRT <213> Leptinotarsa decemlineata <400> 49 Met Leu Leu Leu Gly Gly Leu Vai Phe Leu Vai Aia Vai Vai Aia Asn i 5 10 15 Ser Ala Ser Pro Thr Asn Leu Thr Asp Val Giy Ala Ser Cys Thr lie 25 30 Thr Lys Phe Asn Gin Val Ser Ser Val Leu Asn Ser Cys Thr Asn Val 40 45 Val Val Lys Asn Leu Val Val Asp Ala Gly Gin Thr Leu Lys Leu Phe 55 60 Leu Lys Ala Gly Ala Thr Leu Thr Phe Glu Gly Thr Thr Thr Phe Ala 70 75 80 Tyr Ala Glu Trp Lys Gly Pro Leu lie Trp lie Lys Gly Asn Gly lie 90 95 Thr Val Val Gly Ala Gin Gly His Lys Leu Asp Gly Arg Gly Glu Lys 105 110 Trp Trp Asp Gly Lys Gly Asp Gly Gly Lys Val Lys Pro Gin Phe Met 120 125 Phe lie Gin Ala Thr Gly Gly Ser Val Leu Lys Asn lie Asn Met Tyr 135 140 Asn Cys Pro His Gin Cys Val Gly lie Ser Asp Ser Asp His Val Thr 150 155 160 lie Ser Asn Trp Gin Leu Asp Ser Ser Ala Giy Asn Pro Vai Asn Giy 170 175 Lys Giu Vai Giy His Asn Thr Asp Giy Phe Asp Leu Tyr Lys Ser Asn 185 190 Tyr lie Leu Leu Asp Giy lie Vai Vai Arg Asn Gin Asp Asp Cys lie 200 205 Cys lie Asn Giy Giy Ser His Leu Thr Phe Arg Asn Leu Trp Cys Tyr 210 215 220 Giy Giy His Giy Leu Ser lie Ser Ser Giy Met Ser Met Thr Asp Tyr 225 230 235 240 Asn Leu Asn Vai Vai Ser Asp Vai His Phe Giu His Cys His Vai Ser 245 250 255 Asp Ser Arg Asn Giy lie His lie Lys Thr lie Ala Asp Giy Giy Lys 260 265 270 Giy Lys Met Ser Giy Leu Tyr Phe Ser Asp lie Gin Leu Ser Giy lie 275 280 285 Ser Thr Tyr Giy lie Asn Vai Giu Gin Asn Tyr Arg Asn Asn Giy Giy 290 295 300 Pro Ser Giy Lys Pro Asn Asn Asn Vai Pro lie Asp Thr Leu Giu lie 305 310 315 320 Asn Giy Vai Thr Giy Thr Leu Asn Giy Pro Trp Ser Vai Lys Ala Tyr 325 330 335 lie Leu Cys Ala Ser Giy Ala Cys Lys Asn Phe Lys Trp Ser Asn lie 340 345 350 Asn Leu Gin Giy Asn Ser Arg Pro Asp Ser Cys Asn Phe His Pro Ser 355 360 365 Giy Tyr Asn Cys <210> 50 <211> 366 <212> PRT <213> Leptinotarsa decemlineata <400> 50 Met Ser Leu Leu Ser Val lie lie Ala Ser Leu Leu Val Thr Leu Thr 1 5 10 15 Ala Ala Lys Pro Ser Phe Thr Gly Asp Asn Cys Thr lie Thr Glu Phe 25 30 Ser Gln Val Glu Ser Val Ser Ala Lys Cys Thr Asn lie lie Val Ser 40 45 Asn Leu Gin Val Pro Gly Gly Glu Thr Leu Asp Phe Lys Phe Lys Lys 55 60 Pro Gly Vai His lie Thr Phe Glu Gly Lys Thr Thr Phe Gly Tyr Gin 70 75 80 Leu Trp Lys Gly Pro Leu lie Arg lie Gin Gly Val Gly lie Thr Val 90 95 Thr Gly Ala Pro Gly Ser Val Leu Asp Gly Gln Gly Ala Leu Tyr Trp 105 110 Glu Gly Lys Asn Gly Lys Lys Thr Lys Pro Lys Phe Phe Lys lie Lys 120 125 Val Lys Glu Gly Ser Val Phe Lys Asp lie His Leu Leu Asn Cys Pro 135 140 Val His Cys Val Ser Val Ser Met Ser Asp His Val Thr Leu Ser Gly 150 155 160 Trp Asn lie Asp Val Ser Ala Gly Asp Lys Asp Glu Leu Gly His Asn 170 175 Thr Asp Gly Phe Asp lie Ser Glu Thr Asn Asn lie Leu lie Glu Asn 185 190 Ala lie Val Gin Asn Gin Asp Asp Cys Val Ala Val Asn Gin Gly Phe 200 205 Asn Met Thr Phe Arg Asn Leu Glu Cys lie Giy Gly His Gly Leu Ser 210 215 220 Leu Ser Val Gly Met Ser His Glu Val lie Lys Asn Thr Val Ala Asn 225 230 235 240 Val Thr Phe Arg Asp Ser lie Val Lys Asn Ser Arg Asn Gly lie His 245 250 255 Val Lys Thr His Thr Asn Ser Gly Glu Gly Leu lie Arg Asp lie Thr 260 265 270 Tyr Lys Asn lie Glu Met Thr Gly lie Trp Arq Tyr Giy Val Asn Val 275 280 285 Glu Gin Asp Tyr Lys Asn Giy Lys Ser Thr Giy Asn Ala Thr Asn Asn 290 295 300 lie Pro lie Arg Giy Leu Thr Leu Thr Asn Val Asn Giy Gin Leu Thr 305 310 315 320 Giy Pro Glu Ser Val Pro Val Tyr lie Leu Cys Giy Ser Glu Giy Cys 325 330 335 Glu Asn Phe Glu Trp Ser Glu Val Ser Phe Arg Giy Ala Ser Asn Pro 340 345 350 Ser Ser Cys Asn Tyr Glu Pro Arg Giy Phe His Cys Pro Lys 355 360 365 <210> 51 <211> 365 <212> PRT <213> Sitophilus oryzae <400> 51 Met Asn Ala Leu Leu Tyr Asn lie lie Phe Leu Val Ser Leu Ala Leu 1 5 10 15 Ala Lys Pro Ser Asn Asn Ser Leu Gly Ala Thr Cys Thr Val Ser Ser 25 30 Tyr Asp Asp Val Ala Ser Ala lie Ser Ser Cys Thr Thr lie Asn Leu 40 45 Gly Ser Phe Thr Val Pro Ala Gly Thr Thr Leu Ala Leu Lys Leu Lys 55 60 Thr Gly Thr Thr Val Asn Ile Gln Gly Thr Ile Lys Phe Gly Tyr Gln 70 75 80 Glu Trp Lys Gly Pro Leu Val Gln Val Ser Gly Lys Gln Val Thr Ile 90 95 Asn Gly Ala Ser Gly Lys Phe Asp Gly Glu Gly Ala Asn Tyr Trp Asp 105 110 Ser Gln Gly Asp Lys Gly Lys Thr Lys Pro Lys Phe Phe Lys Ile Ser 120 125 Thr Thr Ser Ser Ser Thr Phe Asn Asp Ile Thr Leu Lys Asn Cys Pro 135 140 His Gln Cys Val Ser Ile Ser Ser Ala Ser Asp Thr Thr Leu Ser Gly 150 155 160 Trp Asn Ile Asp Val Ser Ala Gly Asp Ser Ala Gly Gly His Asn Thr 170 175 Asp Gly Phe Asp Ile Ser Gly Ser Thr Gly Ile Thr Ile Lys Asn Ser 185 190 Val Val Lys Asn Gln Asp Asp Cys Val Ala Ile Asn Gln Gly Ser Asn 200 205 Leu Val Phe Glu Ser Leu Thr Cys Ser Gly Gly His Gly Leu Ser Leu 210 215 220 Ser Val Gly Gln Ser Thr Glu Asn Gly Asp Ala Asn Thr Val Lys Asn 225 230 235 240 Val Val Phe Ser Asp Cys Thr Val Lys Asn Ser Arg Asn Gly Ile His 245 250 255 Ile Lys Thr His Lys Asp Ala Gly Thr Gly Ala Ile Ser Asp Val Thr 260 265 270 Tyr Lys Asn Ile Lys Leu Ser Gly Ile Thr Asn Tyr Gly Ile Asn Val 275 280 285 Gin Glu Asn Tyr Ala Asn Gly Gly Asp Ser Gly Asp Pro Leu Gly Asn 290 295 300 lie Pro lie Thr Asn Leu Asn Leu Gin Ser Val Thr Gly Ser Met Ser 305 310 315 320 Gly Gly Ser Ser Ser Met Ser Val Tyr lie Leu Cys Giy Asp Giy Giy 325 330 335 Cys Ser Asn Trp Ala Trp Ser Giy Val Ser lie Ser Ser Ala Lys Lys 340 345 350 Ser Asn Ser Cys Asn Phe Thr Pro Ser Giy Phe Thr Cys 355 360 365 <210> 52 <211> 362 <212> PRT <213> Sitophilus oryzae <400> 52 Met lie Met Asn Phe Thr lie Thr Phe Leu Leu Leu Ala Asn Leu Ala 1 5 10 15 lie Ala Val Leu Ser Ser Pro Val Leu Asn Ala Thr Cys Thr lie Ser 25 30 Ser Phe Asp Glu Val Ala Asn Val Val Ala Ser Cys Ser Asp lie Val 40 45 Leu Glu Ser Phe Thr Val Pro Ala Gly Thr Thr Leu Asn Met Asn Leu 55 60 Asn Ser Gly Ser Thr Val Thr Val Lys Gly Val lie Lys Phe Gly Tyr 70 75 80 Gin Glu Trp Ala Gly Pro Leu Val Gin lie Tyr Gly Asn Gin lie Thr 90 95 Phe Arg Gly Ala Gly Gly Tyr Phe Asp Gly Glu Gly Ala Asn Tyr Trp 105 110 Asp Gly Asp Asn Lys Asn Gly Lys Val Lys Pro Lys Phe Phe Lys lie 120 125 Lys Thr Lys Ala Asn Ser Ile Phe Ser Asp Ile Thr Leu Lys Asn Cys 135 140 Pro Lys Asn Cys Val Ser Ile Ser Ala Ser Asp Thr Thr Leu Thr Asp 150 155 160 Trp Leu Val Asp Cys Ser Glu @iy Asp Ser Lys Lys Gly Lys Asn Thr 170 175 Asp Gly Phe Asp Leu Ser Ser Ser Ala Asp Ile Thr Ile Arg Asp Ser 185 190 Val Val Met Asn Gln Asp Asp Cys Val Ala Ile Asn Gln Gly Ser Asn 200 205 Phe Leu Phe Glu Arg Leu Thr Cys Ser Gly Gly His Gly Leu Ser Leu 210 215 220 Ser Val Gly Leu Ser Lys Ser Asn Gly Glu Val Asn Thr Val Arg Asn 225 230 235 240 Ala Thr Phe Thr Asp Cys Val Val Lys Asn Ser Lys Asn Gly Met His 245 250 255 Ile Lys Thr His Asn Asp Gly Gly Thr Gly Ala Ile Thr Asp Ile Thr 260 265 270 Tyr Arg Asn Ile Lys Leu Ser Gly Ile Glu Asn Tyr Gly Ile Glu Ile 275 280 285 Gln Glu Asn Tyr Pro Asn Gly Gly Asp Pro Val Gly Asn Val Pro Ile 290 295 300 Thr Asn Leu Asn Leu Glu Ser Val Ser Gly Asn Met Ser Gly Ala Lys 305 310 315 320 Asn Ser Met Ala Val Tyr Ile Leu Cys Ala Asp Glu Gly Cys Asn Asn 325 330 335 Trp Ser Trp Ser Asn Val Ala Ile Ser Asn Ser Lys Lys Ala Asn Ser 340 345 350 Cys Asn Phe Thr Pro Asn Gly Phe Ser Cys 355 360 <210> 53 <211> 364 <212> PRT <213> Sitophilus oryzae <400> 53 Met Lys lie Phe Gly Val Phe Leu Val Val Ser Val Ala Leu Val Tyr 1 5 10 15 Ser Glu Vai Cys Thr Vai Leu Glu Phe Ser Asn Val Gin Asn Ala Val 25 30 G]n Thr Cys Asn Asp I]e Leu Leu G]u Asn Va] Thr Va] Pro G]y G]y 40 45 Giu Thr Leu Lys Leu His Leu Gln Asp Giy Thr Arg Vai Thr Phe Arg 55 60 Giy lie Thr Lys Phe Giy Tyr Ala Leu Trp Giu Giy Pro Leu lie Giu 70 75 80 lie Asn Gly Thr Asn lie Thr Val Ala Gly Glu Asp Gly Ser lie Phe 90 95 Asp Gly Gin Giy Gin Leu Tyr Trp Asp Gly Gin Giy Giu Trp Gly Vai 105 110 Val Lys Pro Lys Phe Phe Thr lie Gin Leu His Asn Ser Thr Met Lys 120 125 Asn lie Tyr Val Leu Asn Thr Pro Val His Cys Val Leu Leu Thr Asp 135 140 Ser Ser Asn Val Glu Leu Ser Asn Trp Thr lie Asp Asp Ser Ala Gly 150 155 160 Asp Lys Asp Vai Ala Pro Glu Lys Tyr Gly His Asn Thr Asp Gly Phe 170 175 Asp Val Phe Asn Ser Thr Asn lie Val Val Lys Asp Ala Phe Val Tyr 185 190 Asn Gin Asp Asp Cys Val Ala Val Arg Ser Gly Asn Asn lie Thr lie 200 205 Glu Asn Phe Tyr Cys His Gly Gly His Gly Leu Ser lie Ser Ala Gly 210 215 220 Trp Ser Asn Asp Ser Phe Phe lie Asn Thr Leu Thr Asn Val lie lie 225 230 235 240 Arg Asn Ser Gin Leu Val Giy Giy Arg Asn Giy lie His lie Lys Thr 245 250 255 His lie Asp Ala Giy Lys Giy Leu lie Ala Asn Val Thr Tyr Glu Asn 260 265 270 lie Thr Phe Ser Glu Met Glu Tyr Tyr Gly lie Asn lie Gin Gin Asn 275 280 285 Tyr Lys Asn Leu Pro Glu Gin Asn Thr Ser Tyr Pro Val Asp Pro Asp 290 295 300 Asn Asn lie Pro lie Lys Asn Leu Glu Leu Lys Asn lie Gin Gly Ser 305 310 315 320 Val Gin Ser Asn Ala lie Pro Val Tyr lie Leu Cys Ala Asp Glu Gly 325 330 335 Cys Phe Asp Trp Asn Phe Val Asn Val Thr Val Glu Gly Thr Lys Asp 340 345 350 Asn Ser Cys Asn Tyr Val Pro Ser Asn Tyr Thr Cys 355 360 <210> 54 <211> 360 <212> PRT <213> Sitophilus oryzae <400> 54 Met Asn Phe Arg lie Val Val lie Val Phe Thr Leu lie Gly Thr Asn 1 5 10 15 Leu Pro Val Lys Ala Glu Asp lie Cys Asn Val Thr Ala Tyr Glu Asp 25 30 lie Gin Asn Ala Val Ser Asn Cys Ser Thr lie Val Leu Lys Asp Val 40 45 Glu lie Pro Ala Asn Thr Thr Leu Glu Leu His Leu Asn Asn Asn Thr 55 60 Glu Leu lie Phe Asp Gly Arg Leu Thr His Val Pro Val Asp Leu Val 70 75 80 Gly Tyr Leu Val Thr lie Tyr Gly Asn Gly Leu Thr lie Thr Gly Thr 90 95 Lys Asp His Val Leu Asp Gly Met Gly Pro Gin His Trp Asn Gly Lys 105 110 Asn Glu Thr lie lie Met Arg Pro Asn Leu Leu Met Phe Lys Val Thr 120 125 Asn Thr Thr lie Lys Asn Leu Asn lie Leu Asn Cys Ala Asn His Cys 135 140 Thr His lie Ser Asp Ser Ser Asp Val lie Val Ser Asn lie Phe lie 150 155 160 Asp Asn Arq Glu Gly Tyr Pro Gly Val Ala Pro Val Gly Lys Phe Ala 170 175 Ala Asn Thr Asp Gly lie Gly lie Ser Gly Ser Thr Glu lie Tyr lie 185 190 Ser Asp Val Val Val Phe Asn Gin Asp Asp Cys Val Ala lie Thr Glu 200 205 Gly Gin Asn lie Phe Val Glu Asn Leu Phe Cys Asn Gly Ser His Gly 210 215 220 Leu Ser Leu Ser Val Gly Gly Gly Asp Val Ser Asn Val lie Phe Lys 225 230 235 240 Asp Ser lie Leu Thr Gin Pro Arg Gin Ala lie His Leu Lys Thr His 245 250 255 Asn Asp Gly Thr Asp Gly His lie Tyr Asn lie Leu Tyr Glu Asn Tie 260 265 270 Leu Phe Leu Asp Ala Phe Lys Met Gly lie Ser lie Gin Gin Asn Tyr 275 280 285 Pro Ser Gly Glu Leu Arg Gly Asn Val Pro lie Thr Asn Leu Thr Leu 290 295 300 Leu Asn Val His Gly Thr Val Leu Ser Asp Ala lie Pro Val Ser Tie 305 310 315 320 Leu Cys Ala Giu Gly Ala Cys Ser Asp Trp Asn Trp lie Asn Vai Thr 325 330 335 Val Ser Gly Thr Gin Ser Asn Asn Glu Cys Glu Asn lie Pro Glu Asn 340 345 350 Met Arg Asp Lys lie Val Cys Ser 355 360 <210> 55 <211> 407 <212> PRT <213> Callosobruchus maculatus <400> 55 Met Thr Leu Giy Giy lie Ala Giy lie Phe Leu Aia Val Val Ser Leu 1 5 10 15 Thr Ala Ser Ala Thr Val Tyr Asn Val Thr Asp Phe Gly Ala Asp Ser 25 30 Thr Gly Lys Asn Ala Ser Thr Thr Ala lie Thr Lys Ala lie Asp Lys 40 45 Ala Ser Glu Asp Tyr Gly Gly Val Val Arg Phe Thr Lys Gly Gin Tyr 55 60 Leu Ser Gly Pro Phe Glu Leu Lys Ser Asn lie Thr Leu Gin Phe Asp 70 75 80 Asp Asp Val Val Leu Lys Phe Leu Asp Asp Pro Lys Glu Tyr Pro Pro 90 95 Leu Thr Lys Leu Leu Pro Asn Gly Leu Thr Phe Ser Leu Pro Tyr Thr 105 110 Pro Leu lie Arg Ala Phe lie Val Arg Asn Val Val lie Arg Gly Lys 120 125 Ala lie Leu Asp Gly Asn Gly Ala Pro Trp Trp Lys Arg Leu Pro Pro 1_3D 135 140 Pro Asn Ala Arg Pro Gin Phe Leu Phe Phe Tyr Gln Ser Tyr Asn Ile 150 155 160 Thr Val Glu Asp Val Ala lie Arg Asn Ser Pro Met Tyr Asn lie Gln 170 175 Leu lie Tyr Thr Asp His Val Leu Leu Lys Gly lie Ser lie Lys Asn 185 190 Pro Pro Ser Tyr Val Asp Pro Gly Pro Asn Thr Asp Gly lie Asn Cys 200 205 Asn Pro Cys Arg Tyr Leu His Val Thr Asn Val Thr Val Asp Thr Gly 210 215 220 Asp Asp Cys Vai Ala Leu Asp Ala Asp Leu Gly Asp Lys Leu Met Giy 225 230 235 240 Arg Trp Pro Thr Glu His lie Leu lie Glu Asn Ser His Met Phe Ala 245 250 255 Gly His Gly Ala Val Ser lie Gly Ser Ala Thr Thr Ala Gly Leu Arg 260 265 270 Asn lie Thr Val Arg Asn Cys lie Phe Asn Gly Thr Asp Arg Gly Leu 275 280 285 Tyr lie Lys Thr Arg Arg Gly Arg Gly Gly Leu Vai Glu Asp lie His 290 295 300 Tyr His Asn lie Thr Met Leu Asn lie Arq Lys Giu Giy Val Ala Tie 305 310 315 320 Ala Ala Val Tyr Asn Gly Thr Asp Glu Gly Met His Glu Arg Asn Tie 325 330 335 His Trp Val Pro lie Asp Asp Thr Thr Pro Phe Tie Arg Asn lie Arg 340 345 350 Tyr Arg Gly lie Arg Gly Asn Ser Lys Leu Glu Ser Leu Phe lie Val 355 360 365 Gly Leu Pro Glu Arg Gin Val Leu Asp Val lie Leu Asp Asp Phe His 370 375 380 Val Gin Ser Glu Lys Gly lie Phe Leu Asn Gin Thr Gin His lie Tie 385 390 395 400 Tie Asn Gly Lys Glu Gln Leu <210> 56 <211> 409 <212> PRT <213> Cailosobruchus maculatus <400> 56 Met Met Met Tie Leu Trp Ser Tie Tie Cys Leu Ser Phe Ser Leu Ser 1 5 10 15 Tyr Tie Gin Cys Asn Ser Glu Tie Tyr Asn Val Tie Glu Phe Arq Val 25 30 Asp Pro Thr Gly His Tie Val Ser Thr Lys Gly Tie Thr Ala Ala Tie 40 45 Arg Glu Ala Ser Lys Asn Asn Gly Gly Val Val His Phe Pro Lys Gly 55 60 Tie Tyr Arg Thr Gly Pro Tie Gin Leu Arg Ser His Val Thr Leu Gin 70 75 80 Tie Asp Asn Gly Ala Leu Leu Leu Phe Glu Asp Asn Gin Asn Leu Tyr 90 95 Pro Pro Val Asn Val Thr Leu Pro Ser Gly Glu Val Tie Ala Leu Ser 105 110 Tyr Thr Pro Leu lie Ser Ala Phe Gly Gin Arq Asn lie Ser lie Thr 120 125 Giy Arg Giy Met Leu Asp Giy Ser Giy Ala Phe Trp lie Lys Leu Leu 135 140 Pro Pro Pro Ser Thr Arg Pro Phe Phe Leu Tyr Phe Val Glu Ser Gin 150 155 160 Glu Val Leu Leu Glu Giy Val His lie Lys Asn Ser Pro Met Tyr Asn 170 175 Val His Phe Lys Asp Thr Ser His lie Thr lie Lys Giy lie Thr lie 185 190 Thr Asn Pro Glu Asn Thr Val Asp Pro Giy Pro Asn Thr Asp Giy lie 200 205 Asn Cys Asp Pro Cys Arg Tyr Leu His Vai Ser Asn Val Thr lie Ser 210 215 220 Thr Gly Asp Asp Ala lie Val Met Lys Ala Asp Met Arg Gly Arg Thr 225 230 235 240 Ser Lys Gin Leu Lys Pro Thr Glu His Val Leu lie Glu Asn Ser Phe 245 250 255 lie Phe Val Gly His Ala Gly lie Ser Met Gly Ser Ala Thr Ala Gly 260 265 270 Gly Leu Arg Asn lie Thr Val Arg Asn Cys Leu Phe Asn Gly Thr Asn 275 280 285 Arg Gly lie Tyr lie Lys Thr Ala Arg Asp Arg Gly Gly Lys Val Glu 290 295 300 Asp lie His Tyr His Asn lie Thr Met Leu Asn lie Arg Lys Glu Gly 305 310 315 320 Val Ala lie Ala Asp Val Tyr Asn Gly Thr Asp Glu Gly Leu His Glu 325 330 335 Arg Asn Val Tyr Pro Gin Pro Val Thr Glu Asn Thr Pro Phe lie Gly 340 345 350 Asn lie Giu Phe Gin Giy lie Arg Giy Asn Ser Lys Leu Giu Ser lie 355 360 365 Phe lie Vai Giy Leu Pro Giu Ser Pro Vai Vai Asn lie Thr Phe Lys 370 375 380 Asp Phe Ser Aia Lys Ser Asp Leu Pro lie Phe Leu Asn Gin Thr Lys 385 390 395 400 Arg lie Vai lie Asn Giy Lys Giu Gin <2i0> 57 <211> 402 <2i2> PRT <2i3> Caiiosobruchus macuiatus <400> 57 Met Phe Vai Giy Ser lie Thr Trp Leu Aia Phe Cys Leu lie Leu Vai i 5 10 15 Aia Cys Asp Thr Thr Vai Tyr Asp Vai Thr Gin Phe Giy Vai Asp Ser 25 30 Ser Giy Aia Arg Vai Ser Thr Ser Giy lie Gin Aia Vai lie Thr Giu 40 45 Aia Ser Lys Asn Asn Giy Giy lie Vai Tyr Phe Pro Lys Giy Thr Tyr 55 60 Arg Thr Giy Pro lie Giu Leu Lys Ser Giy lie Thr Leu Lys Vai Asp 70 75 80 Asp Giu Aia Leu lie lie Phe Vai Asp Tyr Gin Giu Leu Tyr Pro Pro 90 95 Phe Asp Aia Vai Leu Pro Ser Giy Arg Lys lie His Phe Asp Tyr Thr 105 110 Pro Leu lie Arg Aia Leu Giy Gin Asn Asn lie Ser lie Vai Giy Ser 120 125 Giy lie lie Tyr Giy Vai Giy Giu lie Trp Trp Arg Trp Leu Gin Gin 135 140 Thr Giu Lys Arg Aia lie Phe Vai Tyr lie Asp Giu Cys Asp Gly Vai 150 155 160 Leu lie Giu Gly Vai Gin Leu Asn Gin Ser Pro Gin Tyr Gin lie Asn i65 170 i75 Leu Lys Ser Thr Giu His Thr Vai lie Lys Asp Ser lie Leu Ser Ser i80 i85 190 Vai Giy Asp Asp Vai Phe Pro Vai Giy Gin Arq Thr Asp Giy lie Asn i95 200 205 Cys Giu Ser Cys Arg Tyr Leu His Vai Lys Asn Vai Thr lie Arg Ser 2i0 2i5 220 Giy Asp Asp Giy lie Aia Leu Gin Aia Asp Lys Gin Leu Lys Aia Thr 225 230 235 240 Giu His Vai Leu lie Giu Asn Ser Lys lie Leu Ser Giy Pro Ser Giy 245 250 255 Vai Ala lie Gly Ser Asn Thr Ala Giy Giy Val Arg Asn Val Thr Val 260 265 270 Arg Asn Cys Val Val Asn Gly Thr Asp Arg Gly Leu Tyr lie Lys Ser 275 280 285 Ser Arg Gly Gin Gly Gly Thr Val Glu Asp lie His Phe Tyr Asn Val 290 295 300 Thr Leu Glu Lys lie Gly Lys Glu Ala lie Val lie Ala Ser Leu Phe 305 310 315 320 Asp Gly Thr Asp Ala Gly Leu His Glu Arg Asn Leu Glu Pro Gin Pro 325 330 335 Val Thr Asp Thr Thr Pro Phe Val Arg Asn Val Asn Phe Gin Gly lie 340 345 350 Arg Gly Asn Ser Ala Leu Glu Pro Val Phe lie Val Gly Leu Pro Glu 355 360 365 Ala Arq lie Ala Asn Val Thr lie Asn Asp Leu Ser Val Lys Ser Asp 370 375 380 Arg Asn Val Phe Cys Asn Gin Thr Glu His lie Val lie Asn Giy Lys 385 390 395 400 Glu Gin <210> 58 <211> 404 <212> PRT <213> Cailosobruchus maculatus <400> 58 Met lie Ala Phe lie lie Tyr Phe Ala Ala lie Leu Ser Ala lie Ser 1 5 10 15 Ala Ser His lie Tyr Asp Val Asn Lys Phe Gly Ala Asp Ser Thr Gly 25 30 Arg Lys Pro Ser Thr His Ala lie Gin Lys Ala lie Asn Lys Ala Ala 40 45 Ser Asn Asn Gly Gly Thr Val His Phe Pro His Gly Gin Tyr Leu Ser 55 60 Gly Ala lie Glu Leu Lys Ser Asn lie lie Leu Asp Phe Ala Asp Asp 70 75 80 Val Val Leu Lys Phe Leu Asp Asp Pro Lys Glu Tyr Pro Pro Leu His 90 95 Lys Lys Leu Pro Asn Gly Lys lie Val Asn Leu Gin Phe Thr Pro Leu 105 110 lie Arg Ala Phe Asp His lie Asn lie Thr lie Arg Gly Asn Ala Val 120 125 Leu Asp Gly His Gly Glu Thr Trp Trp Lys Arq Met Pro Pro Pro Ser 135 140 Thr Arg Pro Val Phe Leu Gin lie Phe Trp Val Arg Tyr Leu Thr Leu 150 155 160 Glu Asn lie Thr Val Lys Ser Ser Pro Met Phe Asn Val Asn Leu Lys 170 175 Asn Cys Asp Asp Val Thr lie Arg Gly lie Lys lie Arg Asn Pro Ala 185 190 Asn Tyr Val Asp Pro Gly Pro Asn Thr Asp Gly lie Asn Val Asn Ser 200 205 Cys Arg Arg Val His lie Thr Gly Val Asn lie Ser Thr Giy Asp Asp 210 215 220 Cys lie Ala Leu Asp Ala Asn Gly Lys Giy Lys Thr Lys Thr Pro Thr 225 230 235 240 Glu Asp Val Leu lie Glu Asp Ser His Met Tyr Ala Giy His Giy Giy 245 250 255 Val Ser lie Gly Ser Val Thr Ser Gly Gly Leu Arg Asn Val Thr Val 260 265 270 Arg Asn Cys lie Phe Asn Gly Thr Asn Arg Gly Leu Phe lie Lys Ser 275 280 285 Arg Arg Gly Arg Gly Gly Leu Val Glu Asp lie His Tyr His Asn lie 290 295 300 Gin Met Val Asp Leu Arg Lys Glu Gly lie Ala lie Ala Ala lie Tyr 305 310 315 320 Asn Gly Ser Asp Pro Gly Leu Arg Ser Arg Asp Phe Tyr Trp Glu Pro 325 330 335 Val Asn Glu Thr Thr Pro Phe lie Arg Asn Val Glu Phe Lys Asn lie 340 345 350 His Gly Asp Ser Val Leu Asn Pro lie Phe lie Val Gly Leu Pro Glu 355 360 365 Ser Pro lie Glu Asn Val Thr Phe Thr Asn Val Lys lie Glu Ser Lys 370 375 380 Leu Asp Val Phe Thr Asn Asn Thr Lys Asn Val Val lie Asn Gly Lys 385 390 395 400 Val Leu Asp Ser <210> 59 <211> 403 <212> PRT <213> Cailosobruchus maculatus <400> 59 Met Phe Arq Leu Ser Leu Thr lie Leu lie Thr lie Ser Aia Aia Asn 1 5 10 15 Aia Ala Ile Tyr Asn Val Thr Leu Phe Gly Ala Asp Pro Ser Gly Thr 25 30 Arg Pro Ser Thr Asp Ala lie Asn Leu Ala lie Ser Thr Ala Ala Ala 40 45 Asn Asn Giy Giy lie Vai His Leu Ser Ala Giy Ser Tyr Ser Thr Giy 55 60 Pro lie Giu Leu Met Asp Giy Vai Vai Leu Asp lie Ala Pro Giy Cys 70 75 80 Vai Vai Thr Phe Leu Pro Asp Pro Gin Leu Tyr Pro Pro lie Thr Vai 90 95 Lys Leu Pro Asp Giy Lys Leu Arg His Leu Ala Phe Thr Pro Leu lie 105 110 Arg Ala Thr Giy Lys Arg Asn Vai Giy lie Lys Giy Giy Giy lie Leu 120 125 Giu Giy Asn Giy Pro lie Trp Trp Asp Arg Leu Pro Pro Pro Ala Ser 135 140 Arg Pro Phe Phe Phe Tyr Aia Phe Asp Ser His Asn lie Vai Leu Thr 150 155 160 Giy lie Thr lie Arg Asn Ser Pro Met Phe Asn Vai His lie Cys Asp 170 175 Ser Thr Gly lie Thr lie Thr Gly lie Thr lie Phe Asn Pro Pro Asp 185 190 Trp Lys Gly Lys Gly Ala Asn Thr Asp Gly lie Asn Cys Asn Ser Cys 200 205 Arg Gin Leu His lie Ser Gly Ala Thr lie His Thr Gly Asp Asp Cys 210 215 220 lie Ala Leu Asp Ala Gly Gly Lys Arg Asn Lys Arg lie Ala Thr Thr 225 230 235 240 Asn Val Leu lie Giu Asn Cys His Met Thr Ala Giy His Ala Giy Vai 245 250 255 Ser lie Gly Ser Val Thr Thr Gly Gly Leu His Asn lie Thr Val Arg 260 265 270 Asn Ser Leu Phe Glu Asn Thr Lys Arg Gly Leu Phe lie Lys Thr Asn 275 280 285 Arg Leu Arg Giy Gly Leu lie Arg Asp lie Arg Tyr Ser Asn lie Asn 290 295 300 Met Asn Asn Vai Lys Gly Glu Giy lie Ala Vai Aia Met Val Tyr Asn 305 310 315 320 Ala Lys His Gin Asp Tyr His Asp Arg Asn lie Pro Arg Glu Pro lie 325 330 335 Lys Asp Thr Thr Pro Phe lie Tyr Glu lie Glu Tyr Asp Gly lie Ser 340 345 350 Gly Thr Cys Gly Asn Glu Pro Val Leu Leu Val Gly Leu Pro Glu Ser 355 360 365 Pro Val Arg Asn lie His lie Asn Asn Phe Ser Vai Arg Ser Leu Lys 370 375 380 His Lys Giy Asn Tyr Leu Tyr Asn Thr Gin Asn lie Tyr lie Asn Asp 385 390 395 400 Gin Lys Gin <210> 60 <211> 395 <212> PRT <213> Caliosobruchus maculatus <400> 60 Met Vai Leu Leu Leu Ala Thr lie Gin Leu Leu Vai lie Ser Ala Vai 1 5 10 i5 Ser Ala lie His Asp Val Thr Lys Tyr Gly Ala Asp Arg Thr Gly Arg 25 30 Val Pro Ser Thr Asp Ala lie Ala Arg Ala lie Asn Asp Ala Glu Thr 40 45 His Gly Gly Gly Leu Val His Phe Pro Pro Gly Arg Tyr Leu Thr Gly 55 60 Pro lie Glu Leu Lys Ser Asn Met lie Met Asp Val Gly Asp Gly Thr 70 75 80 lie lie Thr Phe Leu Asp Asp Pro Ala Leu Tyr Pro Pro Leu His Gly 90 95 Lys Leu Pro Asp Gly Gin Arq Arg lie Leu Pro Phe Thr Pro Leu lie 105 110 Arg Ala Trp Gly Gin Lys Asn Val Gly lie Arg Gly Asn Val lie Phe 120 125 Asp Gly Arg Gly Glu Val Trp Trp Asp Arg Leu Pro Pro Pro Ala Thr 135 140 Arg Pro lie Phe lie Asn Phe Phe Asp Cys His Asn Val Val Leu Lys 150 155 160 Gly Phe Thr lie Lys Ser Ser Pro Met Phe Asn Val Asn lie Leu Tyr 170 175 Ser Asn Gly lie Val lie Asp Gly lie Arq lie Arg Asn Pro Glu Ser 185 190 Tyr His Gly Lys Gly Pro Asn Thr Asp Gly lie Asn Val Val Ser Val 200 205 Arg Arg Leu His lie Thr Gly Val Asp Val Ala Thr Gly Asp Asp Cys 210 215 220 lie Ala lie Asn Ala Trp Giy His Giy Lys Glu Arg lie Pro Thr Glu 225 230 235 240 Asp Val Leu lie Glu Asp Ser His Met Ser lie Giy His Giy Giy Val 245 250 255 Ser lie Gly Ser Val Thr Ala Gly Gly Leu Arq Asn lie Thr Val Arg 260 265 270 Asn Cys Val Phe Asp Gly Thr Thr Arg Gly Leu Leu lie Lys Thr Asn 275 280 285 Arg Glu Arg Gly Gly Thr lie Glu Asp lie His Tyr Glu Asn lie Val 290 295 300 Met Asn Asn Met Arg Trp Glu Gly lie Ala lie Thr Asp Leu Tyr Asn 305 310 315 320 Asp Lys Asp Ser Gly Arg Lys Pro lie Gly Asp Gin Thr Pro Phe Val 325 330 335 Arg Asn lie Lys Tyr Asn Asn Val Arg Gly Ser Ser Lys Lys Ser Pro 340 345 350 lie Met Leu lie Gly Leu Pro Glu Ala Pro Val Glu Asn lie Glu lie 355 360 365 Glu Asn Cys Asp lie Arg Ser Lys Arg Pro Asn Phe Gin Leu Gin Asn 370 375 380 Thr Arg Asn Val Val lie Asn Gly Arg Arg Tyr 385 390 395 <210> 61 <211> 394 <212> PRT <213> Callosobruchus maculatus <400> 61 Met Leu Leu Leu Ala Ile Ile Gln Leu Leu Val Ile Ser Ala Val Ser 1 5 10 15 Ala Ile Tyr Asp Val Thr Arg Tyr Gly Ala Asp Arg Thr Gly Arg Val 25 30 Pro Cys Thr Asp Ala Ile Ala Arg Ala Ile Lys Asp Ala Glu Ile His 40 45 Gly Gly Gly Leu Ile His Phe Pro Ser Gly Arg Tyr Leu Thr Gly Pro 55 60 Ile Glu Leu Lys Ser Asn Met Val Met Asp Val Gly Thr Gly Thr Ile 70 75 80 Ile Thr Phe Leu Asp Asp Pro Ala Leu Tyr Pro Pro Leu Asp Val Thr 90 95 Leu Pro Asp Gly Gln Arg Arg Ser Val Pro Phe Thr Pro Leu Ile Arg 105 110 Ala Trp Gly Gln Lys Asn Val Gly Ile Arg Gly Asn Lys Val Ile Phe 120 125 Asp Gly Arq Gly Glu Ile Trp Trp Asp Arg Leu Pro Pro Pro Ala Thr 135 140 Arg Pro Ile Phe Val Asn Phe Phe Asp Cys His Asn Val Val Leu Lys 150 155 160 Gly Phe Thr Ile Lys Ser Ser Pro Met Phe Asn Val Asn Ile Leu His 170 175 Ser Ser Gly Ile Val Ile Asp Gly Val Arg Ile Arg Asn Pro Glu Ser 185 190 Tyr His Gly Arg Gly Pro Asn Thr Asp Gly Ile Asn Leu Val Ser Val 200 205 Arg Lys Val His Ile Thr Gly Val Asp Val Ala Thr Gly Asp Asp Cys 210 215 220 Val Val Phe Asn Ala Trp Gly Tyr Leu Lys Glu Arg Val Pro Thr Glu 225 230 235 240 Asp Val Leu Ile Glu Asn Ser Tyr Met Ser Val Gly His Gly Ala Ile 245 250 255 Ser Ile Gly Ser Val Thr Ala Gly Gly Leu Arg Asn Ile Thr Val Arg 260 265 270 Asn Cys Val Ile Asp Gly Thr Thr Arg Gly Leu Asp Ile Lys Thr Asn 275 280 285 Arg Gln Arq Gly Gly Thr Ile Glu Asn Ile Asn Tyr Tyr Asn Ile Thr 290 295 300 Met Asn Arg Val Arg Trp Glu Gly Ile Ala Leu Ile Asp Leu Phe Asn 305 310 315 320 Asp Asn Asp Pro Ser Trp Lys Pro Ile Gly Asp Gln Thr Pro Phe Ile 325 330 335 Arg Asn Ile Arg Phe Asp Asn Ile Arg Gly Ser Cys Asp Arg Asn Pro 340 345 350 Ile Leu Val Gln Gly Leu Pro Glu Ala Pro Ile Glu Asn Ile Glu Ile 355 360 365 Ser Asn Thr Asp Ile Arg Ser Pro Gln Pro Ile Arg Leu Glu Asn Ser 370 375 380 Arg Asn Ile Val Ile Asn Gly Arg Arg Tyr 385 390 <210> 62 <211> 382 <212> PRT <213> Sitophilus oryzae <400> 62 Met Lys Ile Ile Val Leu Leu Leu Leu Ala Val Val Leu Ala Ser Ala 1 5 10 15 Asp Gln Thr Ala Pro Gly Thr Ala Ser Arq Pro Ile Leu Thr Ala Ser 25 30 Glu Ser Asn Tyr Phe Thr Thr Ala Thr Tyr Leu Gin Gly Trp Ser Pro 40 45 Pro Ser lie Ser Thr Ser Lys Ala Asp Tyr Thr Val Gly Asn Gly Tyr 55 60 Asn Thr lie Gin Ala Ala Val Asn Ala Ala lie Asn Thr Gly Gly Thr 70 75 80 Thr Arg Lys Tyr lie Lys lie Asn Ala Gly Thr Tyr Gin Glu Val Val 90 95 Tyr lie Pro Asn Thr Lys Val Pro Leu Thr lie Tyr Gly Gly Gly Ser 105 110 Ser Pro Ser Asp Thr Leu lie Thr Leu Asn Met Pro Ala Gin Thr Thr 120 125 Pro Ser Ala Tyr Lys Ser Leu Val Gly Ser Leu Phe Asn Ser Ala Asp 135 140 Pro Ala Tyr Ser Met Tyr Asn Ser Cys Ala Ser Lys Ser Gly Thr lie 150 155 160 Gly Thr Ser Cys Ser Thr Val Phe Trp Val Lys Ala Pro Ala Val Gin 170 175 lie Val Asn Leu Ser lie Glu Asn Ser Ala Lys Asn Thr Gly Asp Gin 185 190 Gin Ala Val Ala Leu Gin Thr Asn Ser Asp Gin lie Gin lie His Asn 200 205 Ala Arg Leu Leu Gly His Gin Asp Thr Leu Tyr Ala Gly Ser Gly Ser 210 215 220 Ser Ser Val Glu Arg Ser Tyr Tyr Thr Asn Thr Tyr lie Glu Gly Asp 225 230 235 240 lie Asp Phe Val Phe Gly Gly Gly Ser Ala lie Phe Glu Ser Cys Thr 245 250 255 Phe Tyr Val Lys Ala Asp Arg Arg Ser Asp Thr Ala Val Val Phe Ala 260 265 270 Pro Asp Thr Asp Pro His Lys Met Tyr Gly Tyr Phe Val Tyr Lys Ser 275 280 285 Thr lie Thr Gly Asp Ser Ala Trp Ser Ser Ser Lys Lys Ala Tyr Leu 290 295 300 Gly Arg Ala Trp Asp Ser Gly Val Ser Ser Ser Ser Ala Tyr Val Pro 305 310 315 320 Gly Thr Ser Pro Asn Gly Gin Leu lie lie Lys Glu Ser Thr lie Asp 325 330 335 Gly lie lie Asn Thr Ser Gly Pro Trp Thr Thr Ala Thr Ser Gly Arg 340 345 350 Thr Tyr Ser Gly Asn Asn Ala Asn Ser Arg Asp Leu Asn Asn Asp Asn 355 360 365 Tyr Asn Arg Phe Trp Glu Tyr Asn Asn Ser Gly Asn Gly Ala 370 375 380 <210> 63 <211> 379 <212> PRT <213> Sitophilus oryzae <400> 63 Met lie Val Gin Thr lie Thr lie Ser lie Leu Leu Val Val Ala lie 1 5 10 15 Gin Ala Asp His Gin Thr Tyr Pro Gly Ser Ala Ser Arg Pro lie Leu 25 30 Ser Asp Ser Glu Ala Ala Gin Tyr Thr Glu Thr Asn Tyr Leu Gly Gly 40 45 Trp Ser Pro Glu Asn lie Asn Thr Val Gin Ala Asp Tyr Thr Val Gly 55 60 Ser Gly Gin Gin Phe Ser Ser Val Gin Gin Ala Val Asn Glu Ala lie 70 75 80 Asn Ala Gly Gly Thr Ser Arq Lys Tyr lie Arq lie Giu Pro Giy Thr 90 95 Tyr Asn Glu lie Leu Tyr lie Pro Ser Thr Lys Val Pro lie Thr Leu 105 110 Tyr Giy Thr Gly Asn Ser Giy Asp Vai His lie Tyr Phe Asp Gin Ser 120 125 Aia Gln Thr Thr Giy Thr Asp Tyr Aia Asn Thr Vai Asn Pro Asn Giy 135 140 Giu Arg Tyr Lys Ser Giy Asp Pro Aia Tyr Ser Met Tyr Giu Lys Cys 150 155 160 A]a G]y Asn A]a Asn I]e G]y Thr Ser Cys Ser Ser Va] Va] Trp lie 170 175 Asn Aia Asp Asp Vai Gin Vai Thr Lys lie Thr Phe Giu Asn Pro Ser 185 190 Ser Aia Aia Gin Aia Vai Aia Vai Gin Thr Asn Giy Lys Asn lie His 200 205 Phe Glu Asp Vai Gin Phe Leu Giy Phe Gin Asp Thr Leu Tyr Leu His 210 215 220 Ser Gly Lys Aia Tyr Phe Asn Asn Vai lie Vai Lys Giy Asp Vai Asp 225 230 235 240 Phe lie Phe Giy Aia Aia Thr Aia lie Phe Lys Asn Ser Gin Leu lie 245 250 255 Giy Arg Giy Asp Arg Pro Arg Thr Ser Giy Leu lie Phe Aia Pro Ser 260 265 270 Thr Asp Pro Asn Asn Lys Tyr Giy Phe Leu Vai lie Asn Ser Leu lie 275 280 285 Ser Ala Asp Ser Asn lie Giu Gin Arg His Giy Leu Ser Leu Aia Arg 290 295 300 Aia Trp Asp Ser Giy Vai Ser Ser Giy Ser Tyr Vai Pro Giy Vai Ser 305 310 315 320 Pro Asn Gly Gin Leu Vai lie Arg Giu Ser Ser lie Asp Asp Giy lie 325 330 335 Asn Vai Asp Aia Pro Tyr Ser Thr Ser Thr Ser Giy Arg Giu Phe Asn 340 345 350 Thr Asp lie Asn Thr Asn Arg Asn lie Asp Asp Asn Vai His Asn Arg 355 360 365 Phe Trp Giu Tyr Lys Asn Thr Giy Thr Giy Aia 370 375 <2i0> 64 <2ii> 376 <2i2> PRT <2i3> Sitophiius oryzae <400> 64 Met Leu Leu Lys Leu Vai lie Leu Asp Phe Met lie lie Aia Ser Ser i 5 10 15 Cys Tyr His Asn Asn Leu Tyr Pro Giy Ser Vai Ser Arg Pro Vai Leu 25 30 Ser Asn Ser Giu Aia Gin Asn Tyr lie Giu Leu Giu Tyr Leu Gin Giy 40 45 Trp Leu Pro Giu Ser lie His Leu Ser Giu Pro Asp Tyr Ser Vai Giy 55 60 Tyr Giy Giu Pro Tyr Ser Ser lie Gin Thr Aia lie Asn Vai Aia Leu 70 75 80 Ser Vai Asn lie Ser Arg Arg Gin Tyr lie Tyr lie Lys Asn Giy lie 90 95 Tyr Gin Giu Thr Vai Tyr lie Pro Aia Ser Ser lie Pro Leu Thr lie 105 110 Tyr Giy Cys Leu Asp Asp Aia Giu Aia Vai Gin lie Arg Phe Asn Gin 120 125 Ser Aia Vai Thr Thr Giy lie Giu Tyr Aia Ser Thr Vai Asn Pro Asn 135 140 Gly Thr Lys Tyr Lys Thr Gly Asp Pro Ala Tyr Ser Met Tyr Glu Glu 150 155 160 Cys Ala Leu Lys Ser Thr Leu Gly Thr Arg Cys Ser Gly Val Phe Trp 170 175 lie Met Gly Asp Asn Val Gin lie Ser His Leu Thr Val Glu Asn Thr 185 190 Ser Lys Asn Gly Gln Ala Val Ala Val Gln Thr Asn Gly Asp Lys Val 200 205 Gln Phe Asp His Val Asn Ile Lys Gly Phe Gln Asp Ser Phe Tyr Leu 210 215 220 Asn Gly Asn Gly Arg Val Phe Ile Asn Lys Ser Leu Ile Thr Gly Asp 225 230 235 240 Val Asp Phe Val Phe Gly Ser Ala Thr Ala Ile Phe Leu Asn Thr Thr 245 250 255 Phe Ile Ala Arg Asp Asp Arg Pro Arg Asn Thr Ala Ile Ile Phe Ala 260 265 270 Pro Ser Thr Pro Pro Thr Lys Lys Tyr Gly Phe Leu Val Lys Glu Cys 275 280 285 Thr Ile Ser Thr Ser Gly Asn Ile Ser Glu Ser Thr Gly Leu His Leu 290 295 300 Ala Arg Ala Trp Asp Gly Ser Asp Tyr Ile Pro Gly Thr Ser Ala Asn 305 310 315 320 Gly Gln Leu Ile Ile Arg Glu Ser Ile Ile Ser Lys Gly Leu Asn Val 325 330 335 Ser Gly Pro Tyr Ser Pro Ala Thr Ser Gly Arq Pro Phe Met Gly Asn 340 345 350 Ile Gln Lys Asn Arg Asp Leu Asp Asp Val Asn Tyr Asn Arq Phe Trp 355 360 365 Glu Tyr Asp Asn Val Asp Leu Glu 370 375 <210> 65 <211> 390 <212> PRT <213> Sitophilus oryzae <400> 65 Met Gly Ser lie Ser Asn Tyr His Leu Thr Leu Val Ala Leu Val Val 1 5 10 15 Thr Leu His Leu Ala lie Ser Ser His Gin Asn Tyr Pro Giy Thr Glu 25 30 Thr Arg Pro I]e Leu Ser Asp Asp G]u A]a Ser Lys Tyr G]n G]u G]u 40 45 Asn Tyr Phe Gly Asp Trp Glu Pro Glu Glu lie lie lie Pro Asp Glu 55 60 Pro Asp Tyr lie Val Lys Ala Gly Glu Ser lie Gin Glu Ser Val Asn 70 75 80 Ala Ala lie Lys Ala Gly Asn Ser Ser Thr Arg Lys Tyr lie Lys Tie 90 95 Glu Ala Gly Val Tyr Thr Glu Thr Val Tyr lie Ala Gly Asp Val Pro 105 110 Leu Thr lie Tyr Gly Gly Asp Ser Ala Ser Asp Val His lie lie Gin 120 125 Asn lie Ser Ala Asn Thr His lie Leu Asp Tyr Met Asn Leu Val Asn 135 140 Pro Glu Gly Ala Arg Tyr Gin Giu Gly Asp Pro Ala Trp Asp lie Tyr 150 155 160 Arg Glu Cys Giy His Lys Asn Giy Tyr Tie Gly Thr Asn Cys Ser Ala 170 175 Val Phe Val Val Arg Ser Asp Gin Phe Gin Leu Met Arg lie Thr Val 185 190 Glu Asn Gly Ala Thr Asp Ala Gln Ala Val Ala Val Lys Ile Asp Ala 200 205 Asp Lys Val His Leu Met Ser Ser Asn Phe Leu Gly Gly Gln Asp Thr 210 215 220 Leu Phe Val Giy Ala Asn Asn Lys Thr Glu Glu Arg Val His Val His 225 230 235 240 Met Cys Tyr Ile Glu Gly Asp Thr Asp Phe Ile Phe Gly Ala Ala Ser 245 250 255 Ala Val Phe Asn Met Cys Thr Val Lys Val Val Gly Lys Arg Gly Lys 260 265 270 Asn Thr Ala lie lie Phe Ala Pro Ser Thr Pro Pro Asp Arg Ser Tyr 275 280 285 Gly Phe Leu Val Ile Asp Ser Val Ile Thr Gly Asp Glu Val Tyr Leu 290 295 300 Gly Ser Asn Lys Thr Ser Leu Ala Arg Ala Trp Asp Ala Gly Val Gln 305 310 315 320 Thr Ala Glu Asp Tyr Val Ala Gly Ser Ser Pro Asn Gly Gln Leu Val 325 330 335 lie Arg Asp Ser Gin lie Asp Aia lie lie Asp Vai Asp Gin Pro Tyr 340 345 350 Thr Ala Ala Aia Thr Ser Giy Arg Pro Phe Ser Thr Asp lie Lys Thr 355 360 365 Asp Arg Asn Leu Asp Asp Asn Vai His Asn Arg Leu Trp Giu Tyr Asn 370 375 380 Asn Tyr Giy Asp Asp Ala 385 390 <210> 66 <211> 387 <212> PRT <213> Sitophiius oryzae <400> 66 Met Arg Arg Asn Ala Asn lie Phe Val Val Phe Ala Leu lie Ala Val 1 5 10 15 Ser Leu Lys Trp Thr Arg Ala Asp His Gin Glu Tyr Pro Gly Thr Glu 25 30 Thr Arg Pro Val Leu Ser Asp Ser Glu Ala Ser Arg Tyr Thr Gin Glu 40 45 Thr Val Phe Gly Asp Trp Gly Pro Gin Leu lie Val Val Pro Asp Val 55 60 Pro Asp Tyr Thr Val Lys Val Gly Glu Ser lie Gin Lys Val Val Asn 70 75 80 Gin Ala Phe Leu Asp Gly Gly Val Asp Arg Lys Phe lie Lys lie Glu 90 95 Pro Gly Thr Tyr Glu Glu Phe Val Tyr lie Lys Gly Asp Val Pro Leu 105 110 Thr lie Tyr Gly Gly Gly Asn Ser Ala Glu Asp Val lie lie Val Gin 120 125 Asn lie Ser Ala Ser lie Gin Ala Glu Gly Tyr Val Asn Leu Val Asn 135 140 Pro Asn Gly Glu Arg Tyr Gin Glu Gly Asp Pro Ala Trp Asp lie Tyr 150 155 160 Asn Gly Cys Ala Thr Lys Ser Gly Asn Leu Gly Ser Cys Ala Thr Val 170 175 Leu Trp lie Glu Asn Asp Glu Phe Met Met Thr Leu Val Thr Val Gin 185 190 Asn Thr Ala Val Asp Ala Gin Gly lie Ala Val Gin Val Asp Ala Asp 200 205 Lys lie Asn Leu Met Phe Asn Arg Tyr Leu Ala Ala Gin Asn Thr Leu 210 215 220 Tyr Leu Gly Ala His Pro Glu Gin Arg lie His lie His Gly Ser Tyr 225 230 235 240 Val Glu Gly Gin Thr Asp lie lie Val Giy Gin Giy Ala Ala Glu lie 245 250 255 His Leu Ser Thr Val Lys Val lie Giy Asn Glu Asn lie Glu His Pro 260 265 270 Val lie Phe Ala Pro Ser Thr Pro Ala Thr Gin Thr Phe Gly Phe Leu 275 280 285 Val Tyr Asn Ser Thr Leu Thr Gly Asp Ala Ser Tyr Leu Gly Ser Asn 290 295 300 Lys Val Tyr Leu Gly Arg Ala Trp Asp Ser Gly lie Glu Lys Ser Glu 305 310 315 320 Asp Tyr Val Pro Gly Val Ser Pro Asn Gly Gin Leu lie lie Lys Ala 325 330 335 Ser Gin lie Asp Ala lie Thr Asn Val Glu Gin Pro Tyr Ala Pro Thr 340 345 350 Ser Thr Ser Gly Arg Leu Phe Ser Thr Asp Leu Lys Glu Asp Arq Asp 355 360 365 Leu Asp Asp Asn Thr His Asn Arg Phe Trp Glu Tyr Lys Asn Tyr Gly 370 375 380 Asp Asn Ala <210> 67 <211> 375 <212> PRT <213> Cailosobruchus maculatus <400> 67 Met lie Lys Met Lys Val lie Leu Ala Phe Leu Val lie Phe Gly Val 1 5 10 15 His Ser lie Asp Ala Phe Leu Thr lie Arg Asn Asn Ser Phe Tyr Tyr 25 30 Gly Glu Asp Arg Val Phe Leu Ser Gly Ala Asn Ile Ala Trp Ile Asn 40 45 Phe Ala Glu Asp Phe Gly Ser Gly Gly Tyr Ala Lys Val Arg Ser Ser 55 60 Tyr Glu Ser Ala Ile Asp Asp Ile Ser Ser His Gly Gly Asn Val Ile 70 75 80 Arg Val Trp Leu His Ala Asp Gly Arg Trp Ser Pro Lys Trp Asp Lys 90 95 Asp Gly Phe Ala Thr Gly Glu Asp Thr Gln Ser Leu Ile Asp Asp Leu 105 110 Gly Leu Met Leu Asp Tyr Ala Ala Ser Lys Asn Val Phe Val Phe Ile 120 125 Thr Leu Trp Thr Leu Glu Gly Thr Pro Lys Pro Met Met His Leu Tyr 135 140 Tyr Gln Glu Asp Arg Leu Gln Ser Tyr Leu Asp Arg Val Leu Lys Pro 150 155 160 Leu Val Val Ala Leu Arg Asp Lys Lys Ala Leu Ala Gly Trp Asp Leu 170 175 Val Asn Glu Pro Met Gly Ser Ile Ser Gln Thr Gln Val Asp Pro Asn 185 190 Pro Cys Tyr Asp Thr Thr His Leu Lys Asp Ser Gly Ala Gly Trp Ala 200 205 Gly Lys Thr Ile Asp Phe Arg Leu Val Leu Lys Leu Ile Asn Trp His 210 215 220 Ala Asp Ala Ile Lys Ser Val Val Pro Glu Ala Leu Leu Ser Asn Ala 225 230 235 240 Glu Asn Gly Glu Leu Leu Thr Thr Asn Val Cys Glu Lys Cys Arq Asp 245 250 255 His Tyr Thr Asp Glu Cys Leu lie Gly Ala Giy Giy Arg Ala Asn Giy 260 265 270 Thr lie Asp Phe Tyr Ala Met His Ser Tyr Thr Trp Glu Gly Arg Phe 275 280 285 Ala Pro Thr Ser Pro Phe Leu His Asn Phe Asp Phe Tyr Lys Ser Lys 290 295 300 Lys Pro lie Leu Met Gin Glu Phe Ser Thr Thr lie Thr Glu Ser His 305 310 315 320 Asn Ala Ser Trp Asn Tyr Arq His lie Tyr Glu Giy Asp Tyr Vai Giy 325 330 335 I]e Met Ser Trp G]n Tyr Asn G]n Trp G]y Lys Trp Va] Asp Thr Lys 340 345 350 Glu Ser Met Phe Glu Gly Met Gly Ala lie Arg Asn Leu Thr Ser His 355 360 365 Gly Lys lie Asn lie Lys Leu 370 375 <210> 68 <211> 377 <212> PRT <213> Caiiosobruchus macuiatus <400> 68 Met Ser Thr lie Lys Met Lys Val Val Leu Ala Phe Leu Val lie Phe 1 5 10 15 Gly Val His Ser lie Asp Ala Phe Leu Ser Val Arg Asn Thr Ser Phe 25 30 Tyr Tyr Gly Asn Asp Lys Val Phe Leu Ser Gly Ala Asn Leu Ala Trp 40 45 lie Tyr Phe Giy Ser Asp Phe Giy Ser Gly Gly Tyr Ala Lys Vai Arg 55 60 Ser Ala Tyr Giu Ser Ala lie Asp Asp lie Ser Ser His Gly Giy Asn 70 75 80 Ala Met Arq Val Trp Leu His Ala Asp Gly Arq Tyr Ser Pro Lys Trp 90 95 Asp Gln Asp Gly Phe Ala Thr Gly Glu Asp Thr Gln Ser Leu Ile Glu 105 110 Asp Leu Gly Leu Met Leu Asp Tyr Ala Ala Ser Lys Asn Val Phe Ile 120 125 Val Leu Thr Leu Trp Thr Leu Glu Gly Thr Pro Lys Pro Met Met His 135 140 Leu Tyr Tyr Gln Glu Asp Arq Leu Gln Ala Tyr Leu Asp Arg Val Leu 150 155 160 Lys Pro Leu Val Ala Gly Leu Lys Asp Lys Lys Ala Leu Ala Ala Trp 170 175 Asp Leu Val Asn Glu Pro Met Gly Ser Leu Ser Gln Thr His Lys Asp 185 190 Pro Asn Pro Cys Tyr Asp Thr Thr His Leu Lys Asp Thr Gly Ala Gly 200 205 Trp Ala Asn Glu Thr Ile Glu Tyr Glu Lys Ile Leu Lys Leu Ile Asn 210 215 220 Trp His Ala Asp Ala Ile Lys Ser Val Asp Pro Lys Ala Leu Val Thr 225 230 235 240 Ser Ala Asp Asn Gly Glu Phe Thr Thr Thr Thr Val Cys Glu Lys Cys 245 250 255 Arg Asp His Tyr Thr Asp Glu Cys Leu Ile Gly Ala Gly Gly Arg Ala 260 265 270 Lys Gly Thr Ile Asp Phe Tyr Ala Leu His Ser Tyr Thr Trp Glu Gly 275 280 285 Arg Tyr Gln Pro Thr Ser Pro Phe Lys His Asn Phe Asp Phe Tyr Asn 290 295 300 Ser Lys Lys Pro Tyr Leu Met Glu Glu Phe Ser Thr Thr Asn Ser Glu 305 310 315 320 Ser His Ser Pro Ser Trp Asn Tyr His His lie Tyr Glu Gly Gly Phe 325 330 335 Gly Gly lie Leu Ser Trp Gin Tyr Asn Gin Trp Giy Lys Trp Vai Asp 340 345 350 Ser Lys Giu Ser Met Phe Giu Giy Met Ala Ser lie Arg Asn Leu Thr 355 360 365 Ser Asn Giy Lys lie Asp lie Lys Leu 370 375 <210> 69 <211> 375 <212> PRT <213> Caiiosobruchus macuiatus <400> 69 Met Vai Lys Met Lys Aia Vai Leu Aia Phe Leu Vai lie Phe Giy Vai 1 5 10 15 His Ser lie Asp Aia Phe Leu Ser Vai Arg Asn Thr Ser Phe Tyr Tyr 25 30 Giy Lys Asp Lys Vai Phe Leu Ser Giy Aia Asn lie Aia Trp Phe Asn 40 45 Phe Aia Arg Asp Phe Giy Ser Giy Giy Tyr Tyr Gin Vai Arg Ser Arg 55 60 Phe Giu Thr Aia lie Asn Giu lie Ser Ser Asn Giy Giy Asn Vai lie 70 75 80 Arg Vai Trp Vai His Thr Asp Giy Gin Trp Ser Pro Lys Trp Asp Gin 90 95 Asn Giy Phe Aia Thr Giy Giu Asp Thr Gin Ser Leu lie Gin Giu Leu 105 110 Giy Leu Met Leu Asp Tyr Aia Aia Ser Lys Asn Vai Phe Vai lie Leu 120 125 Vai Leu Trp Asn Leu Asp Vai Thr Pro Gin Pro Met Leu His Leu Tyr 135 140 Thr Giu Asp Asp Lys Leu Gin Aia Tyr Leu Asp Arg Vai Leu Lys Pro 150 155 160 Leu Vai Aia Giy Leu Lys Asp Lys Lys Aia Leu Aia Aia Trp Asp Leu i65 170 175 Vai Asn Giu Pro Met Giy Ser Leu Ser Gin Trp Gin Gin Asp Pro Asn i80 i85 190 Pro Cys Tyr Asp Thr Thr His Leu Lys Asp Thr Giy Aia Giy Trp Aia i95 200 205 Giy Thr Thr lie Asn Tyr Gin Asn lie Leu Lys Leu lie Asn Trp His 2i0 2i5 220 Aia Asp Aia lie Lys Ser Vai Asp Pro Lys Aia Leu Vai Thr Asn Giy 225 230 235 240 Giu Ser Giy Giu Phe Thr Thr Thr Thr lie Cys Giu Lys Cys Arg Asp 245 250 255 His Tyr Ser Asp Giu Cys Leu lie Giy Aia Giy Giy Arg Aia Lys Giy 260 265 270 Thr lie Asp Phe Tyr Aia Met His Ser Tyr Thr Trp Giu Giy Arq Tyr 275 280 285 Gin Pro Thr Ser Pro Phe Lys His Asn Phe Asp Phe Tyr Lys Lys Asn 290 295 300 Lys Pro Phe Vai Vai Giu Giu Phe Ser Thr Thr Asn Ser Giu Ser His 305 310 315 320 Ser Pro Vai Trp Asn Tyr His His lie Tyr Giu Giy Giy Phe Giy Giy 325 330 335 lie Leu Ser Trp Gin Tyr Asn Giu Giu Giy Lys Trp Vai Asp Ser Lys 340 345 350 Gin Ser Met Phe Giu Giy Met Ser Ser lie Arg Asn Leu Thr Ser Asn 355 360 365 Gly Lys lie Asp lie Lys Leu 370 375 <210> 70 <211> 374 <212> PRT <213> Cailosobruchus maculatus <400> 70 Met Lys lie Gly Ser Aia Leu Leu Leu Val Vai Leu Cys Leu His Ser 1 5 10 15 lie Asp Aia Phe Leu Arg Vai Gin Asp Lys Lys Leu Phe Tyr Asn Asn 25 30 Asp Gin Vai Phe Leu Ser Giy Ala Asn lie Aia Trp Phe Asn Phe Ala 40 45 Arg Asp Phe Gly Ser Gly Ala Tyr Asp Tyr Vai Lys Pro Arg Phe Glu 55 60 Gin Ala lie Asp Glu lie Ser Asn Ala Gly Gly Asn Val lie Arg Val 70 75 80 Trp Val His lie Asp Gly Gin Trp Ser Pro Lys Trp Asp Ala Asn Gly 90 95 Phe Ala Thr Gly Glu Asp Thr Pro Ser Leu lie Asn Glu Leu Gly Gin 105 110 Leu Leu Asp His Ala Ala Gin Arg Asn Val Phe Val lie Phe Thr Leu 120 125 Trp Asp Leu Asn Val Thr Pro Arg Gin Met Leu His Leu Tyr Ser Gin 135 140 Pro Asp Arg Leu Gin Ser Tyr Leu Asp Lys Val Leu Lys Pro Leu Val 150 155 160 Ala Ala Leu Lys Asp Lys Pro Ala Leu Ala Ala Trp Glu Val Val Asn 170 175 Glu Pro Leu Ala Ser lie Thr Glu Thr Gin Arg Asp lie Asn Pro Cys 185 190 Phe Asp Thr Thr His Leu Lys Tyr Ser Gly Ala Gly Trp Ser Gly Ala 200 205 His Leu Leu Leu Lys Asp Ile Leu Arg Phe Ile Asn Trp His Ala Asp 210 215 220 Ala Ile Lys Phe Val Asp Pro Lys Ala Leu Cys Thr lie Gly Gly Ala 225 230 235 240 Gly Glu Trp Leu Thr Thr Asn Val Ser Pro Val Thr Arg Asp His Tyr 245 250 255 Thr Asp Ala Cys Leu Ile Ala Ala Gly Gly Arq Gln Leu Gly Thr Leu 260 265 270 Asp Met Val Met Val His Thr Tyr Thr Phe Gln Gly Arg Phe Val Ser 275 280 285 Asp Thr Cys Pro Phe Lys Lys Arg Phe Leu Asp Tyr His Thr Thr Lys 290 295 300 Pro Met Val Ile Glu Glu Phe Ser Thr Ala Cys Asn Glu Cys His Asp 305 310 315 320 Ala Val Ala Asn Tyr Arg Tyr Leu Tyr Asp Ser Gly Tyr Ser Gly Ala 325 330 335 Leu Ala Phe Gln Tyr Asn Gly Pro Gly Gln Cys Val Asp Asp His Pro 340 345 350 Val Met Phe Ala Gly Met Ser Ala Ile Arg Asn Leu Asn Tyr Asn Gly 355 360 365 Arg Ile Asp Ile Arg Leu <210> 71 <211> 372 <212> PRT <213> Gastrophysa viridula <400> 71 Met Lys Val Ala Val Val Phe Val Leu Ala Leu Gly Leu His Ser Ile 1 5 10 15 Asp Ala Phe Leu Lys Val Gln Asn Asn Ala Leu Tyr Tyr Asn Asn Asp 25 30 Lys Val Phe Leu Ser Gly Ala Asn Ile Ala Trp Tyr Asn Tyr Gly Trp 40 45 Asp Phe Gly Ser Gly Ala Tyr Ser Asn Val Lys Thr Asn Tyr Gln Gln 55 60 Ala Leu Asp Glu Ile Ser Gln Ala Gly Gly Asn Ser Ile Arg Val Trp 70 75 80 Val His Ile Asp Gly Gln Trp Ser Pro Lys Phe Asp Ser Glu Gly Tyr 90 95 Ala Thr Gly Ser Asp Thr Asp Ser Leu Ile Ser Asp Leu Gly Glu Leu 105 110 Leu Asp Tyr Ala Glu Gln Lys Asn Val Phe Val Ile Leu Cys Leu Trp 120 125 Asn Leu Ala Val Ala Pro Thr Lys Met Leu Pro Leu Tyr Thr Asp Asp 135 140 Ala Lys Leu Gln Ser Tyr Leu Glu Lys Val Leu Lys Pro Met Ala Ala 150 155 160 Gly Leu Lys Asp Lys Lys Ala Leu Ala Ala Trp Asp Ile Ile Asn Glu 170 175 Pro Ile Gly Ser Leu Thr Gln Gly Leu Thr Asp Ser Asn Pro Cys Tyr 185 190 Asp Thr Asn Asn Leu Ile Asn Ser Gly Ala Asp Trp Thr Asn Val His 200 205 Leu Lys Pro Lys Asp Val Leu Lys Phe Ile Asn Leu His Ala Asp Ala 210 215 220 Ile Lys Ser Ala Asp Pro Lys Ala Leu Val Thr Val Gly Glu Ser Ser 225 230 235 240 Glu Leu Thr Ala Thr Thr Ile Cys Glu Lys Cys Arg Asp Met Tyr Ser 245 250 255 Asp Ser Cys Leu Val Gly Ala Gly Gly Lys Ala Leu Gly Thr Ile Asp 260 265 270 Phe Tyr Gln Leu His Ser Tyr Thr Trp Asn Gly Ala Phe Ser Thr Ser 275 280 285 Ser Pro Phe Lys Asn Ala Ala Ala Ala Phe Lys Ser Asp Lys Pro Ile 290 295 300 Val Val Gly Glu Phe Ala Thr Cys Cys Ser Glu Leu Gln Asp Ser Ala 305 310 315 320 Lys Asn Tyr Gln Tyr Leu Tyr Asn Ser Gly Phe Ser Gly Ala Leu Ser 325 330 335 Trp Gln Tyr Asn Glu Gly Gly Asn Cys Ala Asp Pro Lys Ser Val Ile 340 345 350 Asp Gln Gly Met Ser Ala Ile Lys Asp Tyr Thr Tyr Asn Gly Asn Val 355 360 365 His Val Thr Leu <210> 72 <211> 732 <212> DNA <213> Chrysomela tremulae <400> 72 atgaagcttt cagtggttat tttgacaact tacctatctc tggggtattc agccagcgaa ccctctccaa caatcatccc cagacagggt ggcctgagcg gaacaggcat aaccacccgg tattgggact qctgcaaacc ctcgtqctcc tgggctgacc acgtcaccta tacqaaaaat gcgacaccaq taaccagctg ctcaqtggat ggagtcaccq aaatcgacgc cqqcatccaa tcaggctgcg cagaagacgg ctccggcagc tcatacacct gctccaacca gcaacctttc atagtgaact cqaccttagc ttacqqattc gcagcaqcct cttttatcgg cqqaqaagac tacggctggt gctgctcctg catgctgctg tctttccaag gccaacttgc cggcaaacaa atgctagtgc aagtcaccaa cactgggagc ccattgagta ccaaccagtt cgacatcgag attcccggag gaggtgtcgg cttgtatccg tatggttgcg cgacgcagtg gggagcggac ccggaatcgg gctgggggga gaggtacgga ggggtgagct ctgcagagga gtgcagccag ctgccggaat ctttgcaggc agggtqcctt tggaggttcq atttcatgga agqtqttagc aatccgaatq ttacgttcta tcaaqttgaa tgtccttcqq aacttgttgc aatcaccggt tgtgattact ga <210> 73 <211> 729 <212> DNA <213> Chrysomela tremulae <400> 73 atgaactttc tagctatcgg ttcactagtt attttggctt tggccaactg caaggccgat catggctcgg aagaaataat attgaagcag ggcggtctca gcggcactgg taccaccact cgatactggg actgctgcaa gcccacctgc gcctggccgg gccacgtcga ctacaagtct ccggtgaggt cctgcgaagc aaacggcgtc accaccatcg acccagaagt ccagtccgga tgcgagagcg acggaaggtc ctacatctgc accgatcagg gcgggttcgc catcaactcg acgctggcgt acgggttcgc ggctgcgcgc ttcatcaaca ccgagcgcaa catgtgctgc gcatgcgtgc tgttcacctt ccagagcgac gagctcaagg acaagaagat gctcgtgcag gtgacgaaca ccgggaacgc gcccgagtct ggcaccaacc tgttcgacat cgccatgccg ggcagcggcg tcgggtacta cacgcagggg tgcacctcgc aatggcactc tgacgtcagc aactgggqaq atcaatatgg gqqaqtgcgc gaggaqqccq attgctacaa cttqccgaag tggctgtggg acggatgtaa attccggttc gagtggatga aaggggtgtc gaatccaccg gtgtccttcg tcgaggtcga atgtcccaaa aaacttcttt ctatcagcgg ttgtaatcca gttagttag <210> 74 <211> 726 <212> DNA <213> Gastrophysa viridula <400> 74 atgcaattcc tagcagtcgg ttcactggtt attgtggcct tggccacctg tagcgcggaa aacagcccag aaatcatcca gattgagggc ggtctgagcg gcaccggccc aaccacgcgg tactgggact gctgcaagcc cacctgctcg tggccaggca acgtcgaata caagaccccc gtgaagtcct gcgcagccga tggcgtgacc gccaacgacc ccgagatcca gtcgggctgc gaaaaggacg qqcactcgta catctqcacc aaccaagctg gcttcgtggt gaactcgacg ctggcgtacq qcttcgccgc cgccaqgttc atcggcacca agaggaacat gtqctgctcg tgcgtgctgt tctccttcca gcaggagcag ctcaagggca agaagatgct cgtgcaggtg acgaacaccg gcaacgcgcc cgagaccggc accaacttgt tcgacatcgc gatgccgggc agcggcgtcg gctactacac tcagggatgc acttcgcagt ggaactctga tgtgagcaac tggggtgacc agtacggagg tgtcaacagc gaggccgact gttacaagct gccgcaggcg ttgtgggaag qatgtagatt ccgattcacc tggatgcagg gggtttcgaa ccccqatgtg tccttctacq aqgtccaatg tcccaaagaa cttctcgata ttagtggttg tcacccagtt ggatga <210> 75 <211> 723 <212> DNA <213> Leptinotarsa decemlineata <400> 75 atgaaagggt ttgtggtatt gttgattact gtcgcatttt ttgatgcatc aaccggagaa ccttctcctg agatcattcc cgtggaaggt ggtttatctg gtgatggtat aacgaccaga tactgggatt gctgcaaacc atcctqtgga tgggctgatc ttgtaaataa tacccgattt aagacccccq tacatacttg tgccattgac ggtatcgaqa taatcgaccc acaqcaacaa tcgggttgtg cagatgttgg tgcggcttat acttgtagca atcaacaacc gttcgtagta aattctactc tggcatatgg attttcagca gtatccttca ccggtggaga agattaccat atgtgttgct cttgtatgtt actcaacttc caaggacaac tctcgggaaa aaagatgtta gttcaaataa ccaatactgg gagtccatta gcagttaatc agtttgatat agaactccca ggaggagggg taggaattta ccctcatgga tgcatgaaac aatggaatgc ttccgaaacc ggatggggcg atactttcgg cggtgtacat tcggatgcgg aatgtagcga actgcctgct gtactgcaac ctggatgtca ttttcgtttt caattcatgg aaggtgtatc aaatcctcca gtaacgttcc aacaggttca atgccctcga gaactagttg caataacagg ttgcgagtat taa <210> 76 <211> 729 <212> DNA <213> Leptinotarsa decemlineata <400> 76 atgaaactct tggtgatatc attggtcttg gctgccggtt atgcttctcc acacgataga tccccagata taattccaat cgatqqagga gtgaaagqaq acggtgtaac qactcgttac tgggactgct gtgcaccatc ctgcgcatgg gacgaaatcg ttcacaccaa aaacgggatc cccatccaga cctgccaaaa agacggtata acacccagta gaaaagaaga caatgcgcaa tcgggctgcg tggagggtgg ccaagcttac acttgcacca atcaatcccc ctacctcgtc aacgagaccc tggcttttgg attttcagct tcttccttca acggaggcat agatactgcg caatgctgca tqtgcgtact gctctctttc aaagaccagt tggctggcaa gcaaatgctg gtgcaactqa ccaacactgg atctqacttg gggcagaatc acttcgacat cqccattccc ggaggaggag ttggaatatt cacccttggg tgctctactc aatggggcgt ccctgaaaac ggctggggag agagatacgg aggagtaact tcgatagagg aatgtgacga gctgcctgat gttctgcaag aaggctgcag gttccgcttc acgtttatgg aaggtgtttc caatcccgct gtttcgtttt atcaagttaa gtgccctgct gagctgatag atatttcaaa atgtggagat agggattaa <210> 77 <211> 732 <212> DNA <213> Leptinotarsa decemlineata <400> 77 atgaggcacg taattgtttt ggcattgatc atatttggtt atgtatctac atgcaccagt gagccttccc ctgagataat acctattcca ggaggaaaac aaggaacagg ggtcaccact cgattctggg actgctgcaa accatcatgc tcatggaggg gtaacctcag aaatacatcc gcaactcctq taacttcttg cggqatcgat ggcgatactq ccgtggaccc agatttaatg tcggcatqtq acctgcagaa caaaqqaccc gcatatatqt gcaccaatca qcaaccgttt gtagttaact cqactcttgc ttacqqatac gtagcagctt ccttcacagg caqcaccgat taccagctgt gctgcggctg cgtgcttctt tccttccaag gccaacttgc tgggaaacat ttgctggctc aagtgactga cgccggcagc gacttggttg tcaaccagtt tgacatcgct attcctggtg ggggtgtcgg tattggcaat ggatgcaccg aacaatggaa cgcacctcct gatggatggg gagcgcgata tggtgggata agtacagaag aggaatgtga cgagcttcca atccagcttc aqccaggatg ccatttccgg ttccagttca tggaaggagt ccccaatcct gatgttacqt tcatccaagt tgaatqtcct agagagttqq ttgatactac tqqctgtgag ctacgtgttt ga <210> 78 <211> 714 <212> DNA <213> Leptinotarsa decemlineata <400> 78 atggtaactg ctttggcatt cattttattt gcttgtgttg cttcaagcac cgggqaagac tccccagaca tcattcccgt acccqqaggc ttgagcggqa ctggcatcac tacaagatat tgggactqct qcaagccatc atqctcttgg agagaaaacc tcagaaacac ctccqccgac cccgttacct cctgcgcaat tgatggcaac actgtcatcg acccggaagc aatgtccaac tgcggaggtg gtccctctta tatgtgctcc aaccaacaac ccttcgtggt gaactcaacc ctcgcctacg gattcgcagg tgcctctttt acagggaagg ccgactacca gctgtgctgc ggctgcgtac ttctttcctt cacaggagcg ctatcccaca agaaaatggt agtgcaggtg accaatactq qagccgacgc tgttqccaac cagttcgatc ttgctcttcc aggaggagga gtgggaatca acaacggatg catttcccag tggaatqctc ctcctgatgq ctqqqgtaaa agatacggaq qagtcgacac tgaaqctgag tgtgaccaqc ttcctcggca qctccaggat ggctgtcgct tcagatttga gttcatggaa tcagtttcta atcccagtgt caactttgtg caggtagagt gcccgaaaga gatcgtcgac agatctcatt gtaacttgaa gtag <210> 79 <211> 714 <212> DNA <213> Leptinotarsa decemlineata <400> 79 atggaaactg ctttagcact cattttattg gcctgcgttq cttcaagcac cgqqqaatac tccccaqaca tcattcctgt accaqqaggc ttaagcqqaa ctggcatcac tacaggatat tgggactgct gcaaaccatc atgctcttgg aggggcaacc tcaggaacac ctccgccgac cctgttacct cctgcgcaat tgatggcgac actgtcatcg acccggaagc aatgtccaac tgcggaggtg gtccctctta tatgtgcacc aaccaacaac ccttcgtggt gaactcaacc cttgcctacg gatttacagc ggcatctttc acagggaagg cagactacaa actgtgctgc ggctgcgtac ttctttcctt cataqqagcg ctgtctcaca agaaaatgct ggtqcagata accaatactq qagccgactt qqttqtcaac cagtttqatc ttgcttttcc aqqaqcgggc gtgggaatca acaacggatg catttcccag tggaacgctc ctcctgatgg ctggggtaaa agatacggag gagtcgatac tgaagctgag tgtgaccagc ttccttggca gctccaggat gggtgtcgtt tcagatttga gttcatggaa tcagttcctg ttcccaatgt cacctttgtg caggtagagt gcccaaagga gctcgttgat ataagtcatt gcaacttgaa gtag <210> 80 <211> 711 <212> DNA <213> Leptinotarsa decemlineata <400> 80 atgaagatcq cacttctggc tttattcgtt acttatqctq tggcgctccc caqcqataat gaagacatta ttccagtccc tggaggacgt tctggctggg gtaccaccac gcgttattgg gactgctgta aaccttcctg cgcttgggta gagaacatca aaactagaga tatgcatgct gtaaatactt gcgatagtaa aggcaagaac gttttgaaac ccagtgtcaa gtcaggatgc gacggaggaa ctgcttatat gtgcaataac cagcagccat tcgtagtcaa caaaactctt gcttacggtt tcgctgctgc ttccttcacc ggtggtgtaq ataccaacct ttqttgtgcc tgcttccttt taacattcca aqqccagctt tctggtaaqc aattgctagt qcaaaacacc aactctggtg gagatttggg agcgaaccaa ttcgatattg caactcctgg tggtggtgtc ggtatcttca ctagtggatg tcacgaccaa tggaatgcac catggagtgg ttggggagac cagtacggag gagtacattc ggcagaggaa tgtaacactc ttccacaaga gctgcaatct ggatgcaggt tcaggtttga attcatggag aacgtttcga atccccaagt tcaattccaa caggttgtgt qtccgaaaaa acttqttgag ataaccggat gtaatttgta a <210> 81 <211> 732 <212> DNA <213> Leptinotarsa decemlineata <400> 81 atgatcgtca atattatttc tttcttattc gtcataactt cctgtgtgac tgcaggtacc atcgactatt acccgaacgt agaggttata gaaggaggta taagtggaga tgccatcacc actcgctact qqgactgctg caagccatca tgcggatgga gaggaaacct caacqaccac accgccaatc ctgtaacctc ctqtqccgct gatggcgtta cagtactaga ccccqagatt atggcagcct gtgacccggg aaagaacgga acatcttaca tgtgcaacaa ccaacaaaac tacgctgtqa acgacacatt cqcctacgga tacgtcqcaq cttctttcac qqqaqgcgtc gactacagct actgctgcgc ttgcgttctc ctctccttca agagtggttt ggagggcaag tacatgctgg ctcaaatcac caacgccggt agcgacttgc acgccaacca gttcgatatt gctattcccg ggggtggagt gggtctccac aatggctgcg acttacagtg gcactcgggt ccagatggtt gqggcgacag agttatgggt atagatacca gggaggagtg tgacacgctt cttcccgaac cqctgcgtag cgggtqctat ttcagatttq attttatgga ggqcattcct aatcctqatq tctctttcaa acaaqttaag tgtcccaaqq aactgattgc caatactggt tgtaatatgt aa <210> 82 <211> 708 <212> DNA <213> Sitophilus oryzae <400> 82 atgaaagttt tatgcgtgat tttagctgtg gctgcattcg cacgcgccac agatatccat ttgcaaaaaq ttgtcggagg aatcaqtgga gaagctaaca ccacgagata ctqqqattgt tgtaaaccgt cttgttcttg ggctgaaaat gtaaatgctc agtctccagt tgcatcatgt gctgttgatg gagttacagt tctcgaccct tcctcggggt ccggctgtgc tgaagatgga tcaggaatat cgtatgtatg cactaaccaa caaccgtggg ctgttaacga caccgttgcc tttggatttg ccgcggcatc atttagcgga ggtgctgata atagccagtg ttgcgtctgt ctgcaattgt catttttgaa cgttcttccc aataagaaat tgatcgttca agtaacaaac actggaggaq atttagagag caatcacttt gatattcaqc ttccgggagg tqqtqtcggc tacttcactc atggatgtgt tagccaatgg aactgtcccg cgaatggttg gggtgctcag tatggtgqta ttacttcaga gtctqaatgc aatgaattqc ctgaagtatt acaqccagga tgtcatttcc gtttcaactg gttcgaaaac gccgacaatc ctaaagtgac ctttgaacaa gtcgtttgcc catcagaatt gactagtatc accggatgcg ttgtataa <210> 83 <211> 699 <212> DNA <213> Sitophilus oryzae <400> 83 atgaaacttt tatgcttatt tttaqctgct gctacagttq tacaagcaag aqatattcat ttacaacaag tcgtgggagg agtgagtgga tcagggacga ccactagata ttgggattgt tgcaaaccat cttgtgcatg gaaagaaaat gtggaaactg catcgccagt ggcatcgtgt gctgccgatg gagtgacaac tgttgaccct tccacaagat cagggtgtga ttctgatgga tcatcatata catgcaataa tcaacaacca tgggttgtaa acagtacctt tgcttttggt tttgctgctg catcatttac cggagqtgct gacaacagcc aatgttgtat atgtqtgcag ttatcatttt tqagtactat taccqaaaag acattggtcq ttcaagtaac aaatacagga gcagatttag gcagcaacca ttttgatatt caacttcctg gaggtggtgt tggatatttc acaaccggtt gttccagtca gtggaactgt cccgcaaatg gttggggttc tcaatatgga ggaattacct cagattctga atgcagcgaa ttaccggaag ttttacagcc tggatgtcat ttccgtttca actggttcca aaatgccgac aatcccaatg taaatttcca acaagttgtt tgtccttcag aattgaccag tattaccgga tgtgcttag <210> 84 <211> 744 <212> DNA <213> Sitophilus oryzae <400> 84 atgaaaqtqt taattgtttt qactatttac ttagqqqctq ttttctgtgq qtctqtatta cccgagagaa aacctgaaat aaaaaaagtt gtaggcggtt tttctggatc atgtaccaca actcggtatt gggattgttg taagccaact tgtgcatgga aggaaaacat tgataccgat gttggacctg taaaagcttg taacatcgat ggaqttaata taagtgacgt agaggcccaa tctggatgtg gtggaggtag ctcttacatg tgcaacaacc agcaaagtat tattqttaat tccacatttq cttatggatt tgctqccgct gtctttatat caccacctga aaatctttgt tgtgcatgtt tccttgttaa tttccaaaaa ggcgaatggg gtgactgtgc tggaaaacag atgattgtcc aactgacaaa tacaggcggt tctagcagtg ttaatggcac cgaaaacaac attgaaattg ccatgcctgg aggtggcgtt gggtatttta cagaaggatg taaaactcag tggaatgctc cagatcaagg atggggtgaa caatatggag gagttactac tgaagatggc tgtaatgatc ttcctaccca actacaaccc ggatgcaaat tcagatggga attcttaaac ggatgttcca acccgccagc gacctttgaa caagttgttt gtcctcaaga aattqtagat atctcaggct gtaaaacgga ttaa <210> 85 <211> 744 <212> DNA <213> Sitophilus oryzae <400> 85 atgaaagtgt taattgtttt gactatttgc ctaggggttg ttttctgcgg atctgtaata tccgagagga aacctgaaat aaagaaagta gtaggaggtt tttctggatc atgtaccact tctcggtatt qqgactgttg taaqccaact tgttcqtqqa agggaaacac tqataccgat gttggacctq taaaagcttg taqcqccgat ggagttacaq tgagtgacga aqatacccaa tctggatgtg ttggaggcag ctcatacatg tgcaacaatc agcaaagcat tgttattaat tccacattag cttatggatt tgctgccgct gtctttaaaa caccgcctga aaatatgtgt tgcacatgtt tccttgttac tttccaaaaa ggcgaatggg gtgactgttc tggaaaacag atggttgtcc aactgacaaa tacaggtggt tctagcagtg ttaacggcac cgaagacaac attgaaattg qcatgcctgg aggtgqcgtt gggtattata cagaaggatg taaaactcag tggaatgctc cagatcaagg atqqqqtgac cagtatqqaq gagttactac tqaaqatgga tgtaatgatc ttcctaccca acttcaatcc ggatgcaaat tcagatggga attcttaaat ggatgttcca acccaccggc aacctttgaa caagttgttt gtcctcaaga aattgtagat atctcaggct gtaaaatgga ttaa <210> 86 <211> 762 <212> DNA <213> Sitophilus oryzae <400> 86 atgaaaqttc taattgtttt aaccttttgt atagtttacq tcttctgcgq qtctatattg acccagaggc aaccggaaat aaaaaaagta gttggaggtt attcgggatc atgtactaca tctcgctatt gggattgttg taagccaact tgttcatgga aaggaaacac aaacacggat tatggtccag taagagcttg cagcagcgat ggatacaatg tagatgatgg aaatactgaa tctggatgtg ttggaggcag ctcatatatg tgcaataacc aacagagtgt tgttattaac tccacgttaq cttatggatt tgccqccgct atgttcatca gcccccctga aaacatgtgt tgtacttqtt tccttctcag tttccaaaag ggcgaatqqq gtgactgttc cqqaaaacag atggttgtcc aaattacaaa caccqqtgct ggttctaqca actctagcgg tqatcaaaac aacatcgaag ttgccatgcc tggaggtggt gtcggatatt acacagaagg atgtaaaact caatggaatg ctccagacct aggttggggt gaccagtatg gaggtgtaac tactgaagac ggttgttatg aacttcctac tcaacttcaa gatggatgca agtttagatg ggagttcctt aatggatgtt ccaatccacc agcaaccttt gaacaagttg tttgccctca agaaattgtg gatatatccg qctgtaaaat ggaccctgtt tggaattcgt aa <210> 87 <211> 1920 <212> DNA <213> Chrysomela tremulae <400> 87 atgaagtctt tagcaatcat cgtcgtcttc gggctgatcg cccagctaca agcagacagc gtctacgtgc agcgcttcct ccaacagtac aacaaaatgc acgacccggc caatggctac ttctcgaagt gqggcatacc ctaccatgcc gtggagacgc tcatagtcga agctcctgac cacggccacg aaaccacctc cgaaqcctat tcttactgqa tctggctgga ggccatgtac ggcaagqtaa ccaaaaactt caccqqtttc aatgccqctt ggaccaacat qqaqcagtac atcattcccg tgcacgccag ccaaaccggc aacaactaca acgcctggaa gccagccacc tactccgacg agctcgactg gcctagcgaa tacccatctc cgatgcagtt caacgtgccc gtgggctcag accccatcta tcaagagctg gtgagcgctt acggctccga tgacgtctac tccatgcact gqctcttgga tgtcgacaac acgtacggct ttggaaacct ccctqggtcc tgcgaactgq qacctcaagc cgacqqaccc tctttcatca acaccttcca gaqqqgaccc caagaatccq tatggcgcac cattcctcag accacctqcq actctttcaa qttcqgtggc ccgaacggct tccttgatct cttcqtaggc gacaacaqtt acacccagca atqqaagtac tccaacgctc ctgacgccga cgccagagcc gtccaaggcg ctttctgggc atacacttgg gcaaacgaga atgggcaagc cgccgacgtt tcggctacag tcgagaaggc tggcaagctc ggagactacc tccgctacgc tctgtttgac aaatatttca agaggattgg gaactgcgtt ggtccctaca actgtccggg tgggcaaggt aaggagagcg cccactacct gatctcctgg tactttgctt qqggcgccgc tttatqgggc ggatggtcgt ggaggatcgg agatqgggct gcccacttcq qttatcagaa tccattggcc gcatacqctc tatcccatgt qqatqctatg agacccaagg ccgctaccgg cgtttccgac tggcaaacct ccctggacag gcaactcgaa ttctaccagt atcttcagtc cgttgaggga gccttcgctg gaggtgctac caacacctgg aatggcagat acggtactcc cccagacaat ttaaccacgc acactttcca cggtatgttc tacgactggg agcccgtgta ccatgatccc ccgtccaaca gatggtacgg tatgcagccg tggtctgtgg accgactcgc tcagtactac tatgtcactq gggatgccac agctaagacc ctcctggaca aqtgggtgtc ctqqatcatc tccgagatta aattcgaaaa caacaactac ctggtgcctg aatggttgga atggactggt attcctccag aagttcacgt aaccgtaacc aagcgctcta gtgatgttgg taccgcttcc gctactgcca gaactctctc ctattacgcc gccaagagtg gcgataccaa cgccaaggct gttgccaagc aactgttgga tttgatttgg aacctgtacc aqaccgacaa aggcgtttct atggatgaag tcatggatac ctacagtaga ttcaacgagg aqgtgtacgt gccatacaac tgggtgggcc actaccccag cggtqacgtt atccagcatq qcgccacttt catcqqcttg aggtcctttt acaagaatga taaqqactgg cccaaggtcq aqgcttattt gaacqqaggc gaagctccca cgttcaagta ccacaggttc tggtcccaat ccgatgttgc tttggcccaa ggtacctacg gtatcctctt caatgaataa <210> 88 <211> 1929 <212> DNA <213> Chrysomela tremulae <400> 88 atggggatac tqgcgctaac cctagttgtc gccctcgcga ctcaaataga tgccqgcaac gtctacaggg aacgattcat tcaacagtac aacaagatac acgacgacgc gaacqgctac ttctccaaaq aqggcgtacc ctaccatgcg gtcgaaaccc tcctggtaga aqcacccgat cacggccaca tcaccacctc ggaagcctac agttattaca tctggctgga ggccatgtat ggaggcatca cgaaagactt tgcacccttc aacaaagcct ggcagatcat ggaggaattc atcatccccg tgcacgacag ccagtcgacg aactccgcct acaacccctc ccacccggcc acatacgccg ccgaattcga cagccccgac cagtatccct ctctggtcga ttccagtgtg cctgtcggac aagaccccat ctaccaggag ctggtgaatq cgtatggcac tcccqacatc tacgccatqc actggctttt qqacqtcgac aacatctacq gctttggcaa cacccaaggc aactgcgaag gcgggcctta cacttcggga cccagcttca tcaacacctt ccaaaggggc tcgcaggagt ccgtgtggaa gaccatcccg cagccgtgct gcgactcgtt caagtacggc ggtccgaacg gctttctgga cctcttcacc aaggataaca gctacgccaa gcaatggaaa tacactgtag cqcccgacgc cgacgccagg gctattcaag cggctttctg ggcgqcccaa tgggcccaaq aqgtcggtca gttctccgcc atctccgaca ccctcagcaa agccqccaag ctcggaqact acctgcgtta cqccctcttc gacaaqtact tcaaaaaggt cqqcaactgc gtcggcccat qqtcctgcca agqcqqttac agcaaagaca gcgcccacta cctqttgggt tggtacttcg cgtggggcgg ttctctcgac acttcaaacc cttgggcttg gaggatcgga gacggggccg ctcacttcgg ctatcagaat ccgctgaccg cgtacgctct tgtcaacgaa ccaagcttga agcctaaagg agcctcagcc gtcactgact ggcagatctc tctggacagg cagctggaat tctacgaatg gctacagacc tcggaaggag ctttcgctgg tggcgccagc aacagctgga acggcagata cgacacgcct ccttccgaac tcaccggcaa cactttccac ggcatgttct atgattggga gcccqtttat cacgatcccc cgtccaacag atqqttcggt atgcagccgt ggtccgtgga ccgtctcgcc caactttatt acgtcacagg caacagcaga gcgaagaatt tgttggataa gtgggtcagc tgggtgttgt cagagatcac cttccaggga aaccagtact ccattcccgc caccctagaa tggagcggcg ttcctccaaa cgttcacgtc acagtgacag cacacaccaa cgatgtgggc accgcctctg ccactgctag gactctcgca ttctacgccg ccaagtccgg ggaccagaac gccaagaacg tcgccaagca gctgctagac ggcatgtggg aattgtacca gactgacaag ggagtctcca actcggagat tgccgatact tacaaccaat tctcccacga cgtttacgta ccgaatggat ggtacggtca gtaccccaat ggagatgtca tccaagctcc ggctacattt atcggcctca gatcttggta taagaaggat cctgcgtggc ctaaggtgga agcatatttg aacggcggat ctgctcccga attcactttc cacaggttct gggctcaagc tgatgttgcg ttgtcacaag gaacatatgg gatgcttttc aatgaataa <210> 89 <211> 1929 <212> DNA <213> Gastrophysa viridula <400> 89 atggggccat tggcactcac tctgctagtc gccttagtgg ctcacactaa cgccaataat gtctacacga cccgtttcct cacgcagtac aaaaaaatcc acaacgctgc gagcggctac ttctccaaag agggcgtgcc ctaccacgct gccgagaccc tcctggtcga agcccccgac tacggccacg agaccacctc cgaaaccctc agctactaca tctggatgga agccatgtac ggcgccataa tqaacgactt tggacctttc aacaaagctt gggaggttat ggaqqagttc atgatccctq tqcatgacag tcaqcctacc aacagcqqat ataatccctc caqaccagcc acatatgctg ccgaacatga tactcctaat gagtaccctt cgcttgttga ctccagcgtc tcggttggtc aagaccccat ctaccaagag ctagtgagcg cctacggtac ttccgacatc tacgccatgc actggttgag cgacgtagat aacatctacg gatttggtaa cacccaaggc aattgcgagg ctgggccttc tgcttctgga cccagtttca tcaatacctt ccaaagaggt tcccaggaat ccgtatggaa gacaatccca cagccatgtt gcgattcttt caaqtacggt ggtcaaaacq qqttcttgga cctcttcacc aaagacaaca gctacgctag acaatggaaa tacaccgcag cccctgacgc cgacgccaga gcaattcaag ctgcattctg ggcatctaaa tgggctcaac agaacggaaa actctctacg atcactacaa cactgagcaa agccgccaaa cttggggact acttgcgtta cgccatgttc gataaatatt tcaagaaaat tggcaactgt gtcgggccat gqtcttgtca agctggctgg ggtaaggata gtgcccatta tctgctagga tggtattttg cttggggtgg atctctcgat acccagaacq gttgggcctg gagqattgga gatggcgctq ctcactttgg ttaccagaat cctttqqccq cttacgctct cqtcaatgaa caaaacatqc qqcccaaagg tgccacagca gtaactgact ggcaaatttc actcqatagg cagctggaat tctacgaatg gcttcagacc tcggaaggtg ctttcgctgg tggagcgact aacagctgga atggcagata tgacacgcct ccttcaaatc tgaccgccaa tactttccac ggtatgtttt acgattggga acctgtatac catgaccccc cttccaacag atggttcggt atgcaaccgt ggtcggtaga ccggttggct cagttgtact acgtcagcga caattctagg gctaagagta tqttggagaa gtgggtgaaa tgggtgctct cggagattac gttcaacggc aataagtatt cqatccctgc taccctcgaa tggagtqqaq tacctccgaa tqtccacgtc aaagtgaccg ctcacaccaa cgatgtggga actgcttctg ccaccgctag gactttggct tactacgccg ctaaatctgg agatacgaat gctaaaaatg tcgccaagaa acttctggac ggtatgtggg acttgtatca gactaacaag ggagtttcca actctgaaat cgccgacact tatggtcaat tcaagcacga agtgtacgtg ccaaacggtt ggtacggtca gtatccaaat ggagatgtta ttcaagcgcc tgctacattc atcggcatca gatcatggta caaaaaagat cctcaatqqt ctaaggttga gcaacacttg aatggtqqac cagctcccga attcactttc cacaggtttt gggctcaagc tgatgttgct ttgtctcagg gaacttatgg aatgctcttc aacgaatga <210> 90 <211> 1917 <212> DNA <213> Gastrophysa viridula <400> 90 atgaagtcct tagcgattgc cataqtcgtt tgcttgttcq gccagcttca tgqcqacagc gaatacaaaq cqcgattcct tcaqcagtac gctaaaatqc atgactccgc qaatqgctac ttctccaaqt qqggcattcc gtaccactcg gtggagacac tgatggtgga qqcacctgac tatggtcatg agaccacctc tgaggcctac tcttactgga tttggctgga agctatgtac ggcaagatct ccggtaactt cactggtttc aacgccgcct ggcagaatat ggagcagtat atcattccgg tgcacgccag tcaaactgga aacaactacc aaccctggaa accagcgacc tactccgacg agctcgactg gccaagtgaa tacccctccc cgatgcaatt cggtgtgcca gtgggccaag accccatcta ccaggagctg gtgaacacct acggttccga cgatqtttac tccatgcatt qqctcttgga cgtcqacaac gtgtacqqtt ttggtaacct tcctqgttcc tgcgaacttg gacctcaagc cgatggaccc tcctacatca acactttcca gaggggacct caagagtccg tttggaggac cattccacag actacttgcg actccttcaa gtacggtggt cagcacggtt tcctggatct gttcgtgggc gacaacagct acacccagca atggaagtat tccaatgctc cagacgccga cgccagagcc gtacaagccg ctttctgggc tcacacttgg gccaacgaag aqggcaaatc atccqaggtc tcggacacgq ttgcgaaatc cgctaaactt ggagattacc ttcgttacgc catqttcgac aaatacttca agaagatagg aaactgcgtc ggaccttaca actgcgcagg cggacaaggc aaagagagcg ctcactactt gatctcctgg tacttcgcat ggggagccgc tctttgggga ggatggtcct ggaggatcgg agatggtgcc gctcacttcg gctaccagaa tccattggct gcgtatgctc tgtctaatgt agatgccatg aaaccaaaga gtgccacagg ggcctccgac tggcaagcat ctctcgaaag gcaactggaa ttctacgcat qqctgcagtc tgcaqaagga gccatcgctq gaggggctac gaacacctgg aacggtaqat acggaacacc cccaqacaat gtgaccaaaa acactttcca tqqaatgttc tacgactgqq aaccagttta ccatqaccca gcttccaaca gatggtatgg tatqcaacca tggtctctag atcgtctcgc ccaatactac tacgtcagtg gtgaccaaaa agcgaaggct attctggaca aatgggtatc ctggattctc tcggaactcc aattcgataa cggcgactac aaggtgcctg aatggttaga atggtccggt gtgccaccag aagtacacgt tactgtcact agacgatcca gcgatgtagg cactgcttcc gctactgcca gaactctttc gtattacgct gctaagagtg qqgacacgaa tgccaagaat gccgccaagc agcttctgga tggtqtatgg aaacaccaaa ctactaaagg tgtttctgtg gaagaaqtaq ttgactcgta caqcagattc aatgaggaag tttacgtgcc atacaactgg aagggatact accctaacgg cgacgttatc gaacatggag ctactttcat cggtttgagg tcattctaca agaatgatcc agattggtcc aaggttgagg ctcatttgaa cgggggcgag gctccaaagt tcacgtacca taggttctgg gctcaatcgg atgtcgcctt ggctcaaggt acctacggca ttctattcaa tgaataa <210> 91 <211> 1920 <212> DNA <213> Gastrophysa viridula <400> 91 atgaagtcct cggcaatcgc cctattcgtt tgtctcttcg gccaactgca aggtgatggc gagtacaaag cgcgatttct ccagcagtac cataaaatcc atgatcccac gaacggctac tactccaaac agggcgtccc gtaccactca gcagaaactc taatggtaga tatcaccgat catggccacg aqtccacgtc tgaggcctac tcctactgga tctggatgga agctatgtac ggcaagctca ccggcaactt ttccaqcttc aatgcagcat gggaaaacat ggaqcagaac atcattccqq tqcatgcgag ccaaattggc aacaacaatt acaatccgtc qaaqccagcc acctacgctq acgaactcga ctqqcccagc gattatccca tccccttaca qttcqacgta cccgtgggcc aagaccccat cttccaggag ttagtggaca cctacggatc ctacgacata tacgctatgc attggctctt ggacgccgac aatgtgtatg gctttgggaa cctgcctggc tcctgcgaac tgggcccgga agccgatgga ccctcataca tcaacacttt cgaaaggggg cctgaagaat ctacctggag gaccatcccc cagccaactt gtgatacctt caaatacggt gggcaacatg qtttttcaga aatattcgcc aaacagagca gtttcactaa gtcatggaaa tactccaatq ctccagacgc agacqccaga gccatccaaq cagctttctg qqctcacact tgggccaatg aagagggcaa atcattcgag atatcttcta caattgagaa agctgctaag ctaggagact accttcggta cgccatgttt gacaaatact tcaaaaggat aggtaactgc gtcggacctc acaactgccc aggcggacaa ggcagagaga gtgcccattt cttactctct tggtacttcg gatggggagg tgctctttcc ggcgaatgga cctggaggat cggagatgga acggcttttt tcggctacca gaatccgatg gccgcatatq ccctatccaa cgtqactgcc atgaagccta aaagtgccac cqqaqcttct gactggcaqq cttctctcga caqqcagctg gagttctatg gatggctgca gtcggaagaa ggagccatcg ctggaggtgt taccaactcg tggaacggca gatatgacac tccccccgag aatctgactg aaaacacctt ccacggaatg ttctatgact ggcaaccagt gtgtcatgac ccaccaacaa acagatggta tggcatgcaa ccatggtctc tagatcgtct cgctcagtac tactacgtca ccggcgacga aaaagcgaag actgttttgg ataaatgggt aaactqgatc atctcggaaa tcgaattcaa gaacqgtaac ttccaattqc ctgaatggct aqactqgtcc ggagtqccac caaatgtgca tqttatagta gcaaaacgat ccagcgatac tgctacggct tccgctaccq caagaactct ctcctactac gccgccaaga ccggcgatca gaatgtcaaa aacgtggcca aacagcttct cgattgcata tggaaccacc aaactacaaa aggtgtttct gtggaggaag tactcgactc atatagcaga ttcaacgaga cggtgtacgt gcctgagaac ttcaagggat actacccgaa tggcgacgtt atcgaacatg gcgctacttt catcagtttg aggtcttttt acaagagaga tccggaatgg tacaagatag aqgcttattt gaatgqgggc gagattccta ggttcacgta ccataggttc tgggctcaqt cqgatgtcgc tttqqcgcaa ggtacctacq gtattctatt caacqaataa <210> 92 <211> 1926 <212> DNA <213> Leptinotarsa decemlineata <400> 92 atgttactgc ccctggccgc ccttttgatg ggctgcgtgg cccaaatcca cgccggcgtc tacagtgatc gcttcctgca gcaatacaat aaaatacacg accccaaaaa cgggtatttc tcgaaggaag qaattcccta ccactctgta gaaaccctqa tggttgaagg tccaqaccac ggccacqaaa caacttccga aqcccacagt tactacatct ggttggaagc qatqtacggc aaattgacca acgacttctc caagttcaac actgcctggc agatcatgga agagttcacc attccggttc atgacagtca accgacaaac tataaatata acccctcgaa acctgcaact ttcaaccccg aattggacag ccccgatcaa tatcccactc caagtcagcc agaagtgccc gtgggtcagg atcctctttt ccaagaactg caaaacgctt atggtacccc tgacatgtat agcatgcatt qqttgacgga tgttqacaac gtttacggtt tcggcaactc tcctqgcaac tgcttgqcaq qacctggtac tcccqqtcct tcttacatca acacctatca qaqaqgctcc caagagtcca catggaaaac aattccacag ccgacctqtq acaacttcag qtatqgtgga cgaaacggtt tcttggactt gttcgtcagg gacaataact acgttcagca gtggaaatac acatctgctc ccgatgctga cgctcgagcc gttcaggctg ctttctgggc ttctaagtgg gctaccgaaa acggacaatt aaacaccatt tcatctacct tagcgaaagc tggtaagctg ggagactact tgaggtattc cttcttcgac aaatatttca agagaattgg aaattgcgtt agcccttaca actgccccgg tgggtatgga aaagaaagcq cgcattatct gctctcttgg tatgttggtt qqggaggctc tttqtcggat tacaatqqtt gggcttggag qattqgagat ggggcagccc atttcggtta tcagaaccca ctggccgcct acgccttggt gaatgactac aatctcaggc caaggggcgc tactgctgta aacgactggc aacaatctct ttccaggcag ctggagttct acggctggtt acagtctcaa gaaggagcct tcgctggagg agctacgaat tcctggaatg gacgctacga taatcctccc agtgaactaa ccgccaacac tttccacggt atgttctacg actgggaacc agtgtatcat gaccccccct ccaacagatg gtacqgtatg caggcatqqt ctgttgaccg tttqqcacag tactactacq ttagcggcga ttatcaagct aaatcccttc tagacaaatg ggtttcctgg attctctcgg aaattaaatt caacggcgat aattatactc tgccagccac tctggagtgg gatggcgttc ccccaaacgt tagagtgagg gtaagtggat acaccaacga tgttggcact gcatcgggaa cagccagaac tttgagcttc tacgctgcca aagccaacca cgctgaagcc aagaatgtag ccaaaaagtt attagacgga atgtggaagc tctaccaaac cgataaagga gtttctactc cagaggaagc aagcacctac aatcagttta accttccaat cqccqttcct tacggatqqa ctggccgata tcccaatgga gacgtcatcq ataactcagc taccttcatc aaactccqtt cttggtacaa aaacqatcct gattggccaa aggtggaagc tcatctcaac ggaggtccag cacccgtatt cacttaccat cgattctggg ctcaagctga ccatgctttg gctcagggaa catacggatt acttttcaat gaataa <210> 93 <211> 1932 <212> DNA <213> Leptinotarsa decemlineata <400> 93 atgctcctaq qcgttatgaa qacttttatc gttttcctcq tgggatgcgt ctccctcata aacgggggtg aataccttaa ccgtttcacc caacagtata acaaaataca cgatgcagca aacggatatt tctccccgaa aggaattccc taccacgcgg tcgaaactct aatagtagaa gcacctgact atggccacca aactacatct gaagcctata gttattggct ctggttggaa gccatgtacg gtgcggtcaa cggtgatttt tcaaaattca ccgcagcctg gcagaatatg gaaacctata tqatcccaaa ccatqcatct cagccaacta acaactacta caaccccggt aaacccqcca ccttcgctcc cqaaqqagat taccccaatc aatacccttc qcaaatgcag ttcaacgtac ctgtcggtca ggatccaatc taccaggagc ttgtaaacac atatggtacc agtgacgtct acctcatgaa ctggttgtta gatgtcgaca acaaatatgg tttcggaaac caaccagggc agtgccaact tggaccatca gtagaaggac cttctttcat gaacagtttc caaagaggtc cccaagaaag tgtgtggaga accatccccc aaactacttg tgacaatttc agattoggag qtcagaacgg tttcttggac ttattcgtaq gagacagtca ctacqaacag caatggaaat acactatcgc ctctqatgca gaggctcqtq ctattcaagc aqctttctgg gctttgcaat qqgcaaaaga taaqaaccaa cagggggctq tttcagacac catatccaag gccagtaaaa tgggagattt cttgcgttac gccttcttcg ataaatactt caaaaagatt ggaaactgca ttggaactta tgcctgccct ggaggatatg gcaaagacag tgctcattat ttgctctctt ggtacatggc atggggaggc tccctctacg gaaactgggc ctggagaatt tcagatttct atgcccactt tggttaccaa aacccaatga cggcttatgt catgtctaag gttgatgaat tcaaaccaag aagccctact ggtgtttctq actgggaaat gtccctcgaa aggcagataq aattctacga atacctgcaa tctaaaqaaq gagctttcgc cqqtqgtgcc acaaactctt atgaaggacg ttacgaaact ccgcccgcta acctaatgaa caatactttc cacggtatgt ggtatgaatg ggagccggtt taccacaacc caccctccaa cagatggttc ggtatgcaag cttggtctac tgaccgattg gcccagtact actacgtcag tggagatgaa aaggtgagac ctcttctgga caaatgggtt aactgggttc tcccagaaat ttcttacgac gctaacagct acaagattcc cgaatqgctt gactggtccq gcgttccacc agcaqttgac gtgtccattq aaaaatacgg tactqatatc ggttctqctt ctgcactggc taqqactctc tcttattacg ccgccaagac cggagaccag aaagtaaagg ctgtcgctaa gggtttattg gatggtatct ggaataatca tcaaaccacc aaaggagttg ctatggaaga ggttatggac gcttattctc aatttaacga agaggtttat gtacccccag gttgggtagg ccactaccct gatggaaccg aaatcaaggc ccccactacc ttcattggta tccgtccatg gtacaagaat gaccccgatt qqcccaaagt tgaaqcttat cttaatggcq gtcctgctcc aaaattcact taccatcqtt tctggcaaca gtqtqatatc gctattqccc agggaactta cqqactctta ttcaatgaat aa <210> 94 <211> 1923 <212> DNA <213> Leptinotarsa decemlineata <400> 94 atgaagttgt tatcaatcgc cctggctttg gcgggtctga cactcgtcaa ttgcgccggt gtatataagc aqagattcct cgagcaatac aataagattc acaatgaaaa taatqggtat ttctcaaaag aaggcgtgcc gtatcatgcc gtcgaaaccc taataatcga agcqtgcgac catggtcacc aaactacgtc cqaaqcgtac agctactaca tttggctgga aqctatgaac ggcgccatca gcggaaactt ctcgtcgttc aacgttgcct ggaagaacat ggaagattat attattccgt cccacacaag tcaaccgaac acggacttgt acaaccccaa gaatccagca acgtacgcgc ctgccatgga caccccagaa caatggccaa catctctaga acccggagtt cccgtaggcg aqgaccctct tttccaagag ctccaaacca cctacgacac ctacqatgta tacgcgctgc attggctcct cgacqtagac aacacatacq gcttcggtga taqccaaggt acttgttcqq qtggccccag tqtqaagaag ccatctttta tcaacaacta cqqaagaggg ccgtcagaaa atgtctggat ggccatcccc tcgggaatct gcgaggcatt caagtacggt tccaagacag ggttctctgg catctattcc aaaggtaatc ccgttccgaa ttggaagttt tccgtcgctc ccgacgcgga tgcccgagcc atcctgggag ccttctgggc ctctaggtgg gctaccgaaa aqggggagct gtcccagata acagacactt tggcgaaagc tagtaagttg ggagactatc tacggtacgc catqttcgaa aagtacttca agcgggtggg gaactgcatc gacccttaca aatgcccggg qqqacacgac cgagaqaqcq cgcactacct qctctcctgg cacgtcggat qqggtggctc cgtctctcga aacggcgqqt atgcttggcg aqttqcagat gctaccgtgc attttggata tcagaatcct ttagccgcat acgctttgtc ccaagaaccc actttgaagc caaagagccc gaccgcggcc caagattggg ccgcctcatt ggaaaggcaa ctggaattct acgaatacgt tcagtcggca gaaggagcct ttggaggagg agtcgtcaat tcttggaaag atcgatacga caccccgcca gacgacatag catcgagcac tttccacgga atgttctacg actggcaacc aggtgqtcac aatccgccat ccaccaggtg gttcqgcatg caaccttgqt ctacggatcg gttaqcgcaa tattactacq taacaggtga caaaaaggcc aaggcgattt tggacaagtg ggtgtcatgg gtcatttcgg aaatcacctt tgagggcgac aattacaagc tccctaactc gctggagtgg agtggagtcc ctccgaatgt ccacgtgaaa gtcgctgact acggcagcgg tattggaccg gcagctgcta cggctagaac actgtcttac tacgcggcca agtccggaaa caaagcagct aaacaagttg ctaagaagct tttggacgga atgtggaagt tqtatcgaac caacctaggt gtatcgtccq aagagcagcc tgactatgat catttccaaq ataaagttta cqtacctccg ggatggaqcq gcgtctatcc caacqgagac gtcttgaact cctcgtcgac tttcctcagc atccggtcgt tttataagaa agatccacaa tggcacaaga ttgaagcacg tttgaacggg ggccctgctc cgacgtttac ttaccatcgt ttctgggatc agtcggaggt tgctctagcg atgggggcct acggactttt atttaatgaa taa <210> 95 <211> 1914 <212> DNA <213> Sitophilus oryzae <400> 95 atgagagtat ctctggcctt tttqqccatt gtctgcgtqq tgggtatcca tqqtqgtacc tacactgacc gtttcttgga acagtacaac aaaatccacg attctgctaa tggatacttt tcttctgaag gtattcctta tcacagtgtc gaaacattga tcgtagaagc cccagatcat ggtcatcaaa ccacttctga agctttcagt tactatgtct ggcttgaagc tatgtacgga gccgtcaatg gagatttttc ttccttcaac actgcctgga ctaccctaga aaattacgct attccagcac tqcaaacagc taacgcagct tataatccca gcaaaccagg gtcqtacgaa gctgagttqq acgatcctag cgactaccca tcaccgatcq atttcaacgt tccaqttggt caagatccta tcgctactga actgaagaat gcttatggtt ctgatgtatt gtacgccatg cactggctcc tcgacgttga caatgtttac ggattctcca acactcaagg acagtgtgaa gacggtacta atggtccatc attgatcaac aactaccaga gaggacctca agagtcagta tggaggacag tccctcaacc tacttgcgac caattcaaat acggtggtca aaatggtttc ttggacctgt tcaccaagga caactcttat gctcatcaat ataaatacag tgctqctcca gatgctgacq ccagagctgt ccaaqctgct tactggqctq cccaatgggc tacaqacaac ggacaaattt catctattac agacacatta tccaaagctg ctaagttggg agattacctt agatattcct tatacgacaa atactttaag aaggttggta actgcgttgg tgcctacgct tgccaaggag gtaccggaaa agaaagtgcc cactacctca tcagctggta cttcgcctgg ggtgcctcct acaacgccca gtacgactgg gcctggcgta tcggagatgg cgcagctcat ttcggctacc agaaccctct ggccgcttac gctctgagta ctgactcgaa tctaactccc aaaggctcca cagctgtcga agactggaaa acttccctgg acagacaatt ggaactgtac gagtacttqc aaacttccga agqaqcgttc gctggtgqtq ttaccaactc ctqqaaagga aggtacgata ctcctgactc tgatttactc gtggacactt ttcatggtat gttttatgat tgggaacctg tataccatga ccctccatca aacagatggt acggaatgca accttggtca gctgaccgtc tagctcagta ctactacgta accggagata cgaaagctca agctatcttg gagaaatggg ttaattgggt tattcccaac atcaaatgga cgggagatga ctttgaaatg cccgcgaatc ttggatggac aggagaccca ccaaatgctc acgttaatgt tacttcgtgg ggtaaagatt tqggtacagc ttctqccact gccagaqctt tctcctacta cqctqctaaa gccaacgatg ccaccgtaaa agaaaacgcc aagaagattt tagacgcttt gtacgctaac taccaaacca ccaaaggtat ttcagctccg gaaacccgtg aagattacag ccgttttaac gaagcagtgt atgttcccag cggatggacc ggtacttatc ccaacggtga cgtcatcgat tcgtctgcca cctttatcaa gatcaggtcg tggtacaaga acgatcctga ctggtctaaa atacaggctt acttggatgg tggtqatgta cctacgttta cctatcacag gttctggact caggctgata tcgctctggc ttttqqcgca tacggacttc tttttaacga ataa <210> 96 <211> 1113 <212> DNA <213> Chrysomela tremulae <400> 96 atgttctccc tcagactttt cagtgtcatc gccacagcgt tcgccgcagt ttccgccctt ccgacacaaa acgaaacctt atcgcttggt ggtagctgca ccatcaagga attcagtcaa gtcagctccq tqattagcag ctgcacaaac atcgtgattt caggactgag cgtacccggc gggaaaaccc tqgacctcaa qctqaagaaa gggaccactc tgaccttcga aqqcaagaca gtcttcggqq tcgcccattg ggatqqacct ctccttcaqa tcagcggcga qaaqatcaca gtgaagggag cttcaggtag cgtcctcgac ggccagggag cgaaatactg ggacggcaaa ggcgatgggg gtgtcaaaaa acccaaattc gttcgtgtca aggcatctgg gggctcagtt ctcaccggtc tccatgtctt gaattgcccg cgacagtgtg tgtctgtcaa cagctgtgat cacgtcactc tcagcaattg gaatgtcgac gtttctgctg gagacaagaa caaactgggc cacaacaccg acggcttcga catctcctcc accagcaacc tgttgatcga aaactccgtg gtgaagaacc aagacgactg cgtqqccgtc aataagqcqc aacacgtgac catatcgaag ttgcaatgct ccggcggcca cgggctcagc ctgtccgtcg ggatgagcaa aacctccgcc gaggccaacg tcgtaaaaga cgtccatttc gtggactgca cggtcaccaa ttccgacaat ggcatccaca tcaagaccca ctctgacgga gcgcacggga ccatcgacaa tgtttcctat aagaacatcc atctgtcggg aatcgccaaa ttcggagtca acatccagca ggactacgaa aacggtcatt ccaccggaaa gccgaagaac aacatcccca tcaccggatt gagcatcacc ggagtcaccq qatcgatgac cqqatcgaat tccaagqcqq tatacatttt qtqcqcctca ggagggtgct cagggtggaa ctggggcgtt tccatctccg gagccaagaa ggccaacagt tgcacccacc aaccgtccaa tgtcaaatgt tga <210> 97 <211> 1095 <212> DNA <213> Chrysomela tremulae <400> 97 atgtatcccc ttaccatagt tgtcqcttcc cagctggtac tagcaggcct ggcactagcc cagccctqca ccatctccag atactcggac atccaqcaqq tggtcaactc ctqcaccaac attctggtat cqaatctgac gatqccagct gggatagtqc tggagcttta cctqcagaac aaaacgacca tcaccttcga aggcaccact ttgtttgagt acacgccttg gtcgggccct ctgataaggg tgacaggggt ggacgtcacg ttcgaagggg ccccaggttc tgtcttcgat ggacaagggc ctttgtactg ggatggtaga ggagataaag gtccaaaaaa gccgcagttc atgaagatag aagtgtttgg caaatctgta ttcaggaaca taaaattgct gaactgccca catcactgtg tttacattgg caagtctgat ggcctcaccq tctcgaactg gacaatcgac aattcagcaq qagacttgca caacttcacc ggtcacaaca ccgacggctt cqacqtttcg gcagcgaaga acctcctcat cgaaaacacg gccgtcctca atcaagacga ctgcatagcc atccgatatg gctccaacat catcgtcagg aacatgtact gctatggagg gcatggtctc agcctgtccg tgggcttcaa ccacacctcg ttcccagaga acgtggtgga aaacgtcgtc attgaggata gcctgctggt cgggtccgcc aatgggatac acgtcaagac ccataccgat gggtacctgg qqctcgttag aaatatcacc tacagaaaca tagaaatgaa agatatcacc aattatgqca tcaacgttca gcaaqactac tgcaacqqtt ctagtaccgg aacaccaact tctaacatac ccatagaagc tttgaacata tacaacgtca ccgcttctgc tcaaaaggga tcgaaagcca tgggggttta tatcatttgt ggagaagact cgtgtagaaa ttggacttgg agtggaatcc acttcgaagg tgctgctaaa aaggattctt gtaatttttc cccccaaggg tataagtgtc gataa <210> 98 <211> 1122 <212> DNA <213> Chrysomela tremulae <400> 98 atgattctcc atctgaaatc atttqtgttt ttattaataq ttgtagttag tataqctcac ggttacgaac cgattgatta ccaaacagct aagaacctca ccgcagacta ttgctatgtg aacagctata atgaggtgga ttcaattgtg aaaagttgta gatacatcgt catccagggc ttcgaagttc cagctggtaa aactctgaac ctcaatttgc aaaagaagac tgtactcctg ttcgagggta cactgacttt tggtatggca tactgggacg gtccacttat atcaatctct ggatatgata ttcaagtcat gggagqaaca aatcacctca tacatggaca tggtqaaaaa tactgggatq qtcaaggtga tgqaqqtgtg aaaaaaccac ggaaggtgat qacqatcaac gctcatggtg gatcattcaa aaacattaat atcaaaaatt gtccattgtt ttgtgttgcc atcgttggaa ctgatctcac cttctctgga ttcaatatcg atctccagga tggattcaat aataacctcg gaaggaacac agacggtatg tctttctccc agtcgaacaa catcttaatc gaaaactgta agatctggaa ccaggatgac tgtgtgaacg ttttgggagg tagcaccaat tcactagtca qaaatgttca ctgttqggga tcccatggat tcagcatcac ctcqqggatg gccgattctq aqaaacttaa caqcatcagc aatatcactt tcgaagattc ttctatcggc ggatcattgg gtggaattca cgtcaagact cttcccaccg gtggaccggg aatattggac cgaataactt atagaaatat tcaaataaca gatgtgacaa gatttgccat cgaagctcac caagactacc cgaacgaaga tggtaaatcg tcgaacaata tcaaaataac aaatttgaat ctgatcggtg tcactggaac agtcaaaggt tccagtacaa gaagtgtgcg catcaattgt gggtctggat cqtgttccaa ctggaattgg tcaggaatca agataactgg tgctcagaat aaagacgaat qtaacttcca tcccaatgga tattcatqtt ga <210> 99 <211> 1131 <212> DNA <213> Chrysomela tremulae <400> 99 atgatttctt taatagaagc atttgtgagt ttgctagtaa tcgtagtcag cataaaagct gattcgaatg gttacgagcc gattgattac caaacagcta aaaacctcac cgcagactat tgctatgtga aaagctacga agaggtagat tcagtcgttc agaattgcaa tcgcatcgtt ctagagtcct ttgaagttcc ggctgqtaaa actttgacgc tcaaattgag gaaqaaggct gtactcctqt tcgaaggtac tctqacattc ggtatgqcat actgggatgg ttcactcatt gaaattcgtg gagaaaatat tcaagtcatc ggcggaacaa atcacctcat tcatggacat ggcgaaaaat attgggatgg tcttggtgtt gctggtgttg ttaaaccgaa gagattgatg tcgatcaatg tcaaaggtgg cactttcacg aatatcaata tcaagaattg tccagttttt tgtgtagcca tcatgggaaa tgatctcacc ttctctggat tcaatattga tcttcaggat ggattcaaga atagcttggg taggaacaca gacggtatgq gattctctca cgccaacaac atcgtcattq aaaactccaa aatcaccaac caggatqact gtgtgaacgc cctqqgagat agcagcaatg cggtcgtcag aaatgttcac tgttggggat ctcatggctt cagcgtcacc tcaggaatgt cgacaacaaa tactggcaac gatatcagaa atataacttt tgaggattgc tctatcgggg gatcattagg tggcatccat gtgaagactc ttcctaccgg tggacatggg acattagatc aaataaccta caggaatatc gagataaaag atactacaag atttgctatc gaagttcaac aagattaccc gaaaqaagat ggtcattctt cgaacaatat cattataaat gatttgaatt tqatcaacgt tactqqatca gtcaaqqqta aatcagcaag aqqaqttcgc atcagctgtq qatctggatc ttqcacaaac tggaaatqqt ctggtgtcaa qataacgggt gcacacaaaa aagacgattg taatttccat ccgaatggat attcatgtta a <210> 100 <211> 1125 <212> DNA <213> Chrysomela tremulae <400> 100 atgattcctc acatgcattt cttactgggt ttcttgatag ttattgttgt tgatatagca gctactacaa ctagttatga atctqtcgat tatcaaaaaq ccaagaatct cactqcagac tattgctatq caagaagtta tqccqaggta ccagatatca tcaaaaagtg taaatacatc gtgatagtga acttcgaagt tcctgctggt aacactttgc agcttcacct gcagaagaat tccgtgttat tgatcgaagg aacgataacg tttggtgtgg caaattggga tggcccactt atggatattg caggagagaa tattcaagtc attggaggaa ctaatcacct gattcatgga aagggtgaaa aatattggga tggccatggt ggcggtggtg ttaaaaaacc gaagagattg atgtcgatca atgtccaccg cggatctttc acgaatatca atatcaaaaa ttqcccaatg ttttgtqttt ccatcatagg aaatqatctc actttttctq ggttcaatat cqatcttcag gatggattca agggtggatt agccgttaac actgacgcta tgggattcgt gtattctgac aacatcctca tcaaagactc gaaaatttgg aatcaagacg actgcataaa cgtcatggcc agcaaaaatg cagtcatccg gaatgttcac tgttggggat ctcatggtct cagcttgagt tctggactct caaaaactga cgaaaataat gatatacata atattactta tgaagattgt tccatcgggg gttcattgaa tggtattcat attctgacga tgccttctgg tggcaaagga aaaatcagcc agatcactta tcgaaatatc gaaattaaag aggttacaat gagagggatc gaattccgtc aagattactt agatqatgga catccgaqca acaatatcaa aatcqatggt ttgactctgg agaacattca cggaaccgta accagtggtg cagtgggagt ttacatccaa tgtggttccg gatcatgcac aaactggaat tggtcggggg tgaaaatgac tggatctaag aagaaagacg tttgcaattt ccatccggag gggtattcat gttga <210> 101 <211> 1131 <212> DNA <213> Chrysomela tremulae <400> 101 atgatacatt tttcgaaatc aqttqtgact ttattaatqq ttttagtagg cacatcagca actctcgttg gctatgaatc tattgattat gcaacagctc ttaatcttac tgcggactat tgctatgcca ggagttatga agaagtacca tccattgttc agagatgtga atatatcgtt cttgtggatt tcgaagtccc tgctggtgaa actctcgagc tcaacttgca gaagaattct gtgttattat tcgaaggaac cattaaattt gcagtggcat actgggatgg tccacttatt catataacag qaaataatat ccaaqtaatt ggtgggacqa aacatctcat ccatqgacaa ggtgaatttt attgggatgg tcaaqqaggc tctgqtqqaq tgaagaaacc tqaqaaagta ttctcggtga atgtaaatgg gggaattttc aaaaatatca atatcaagaa ctgtccaacg gcttgcgtta aaatatatgg gaatgattta actttctctg gatttaatat cgatctcaat gatggattca agaataaatt ggccaaacac acagatgcga tgacattctc ccactccaac aacatcgtca ttgagaactc caaaatcgtt aatcagggtg attgtgtgaa cgttatttgc agcagcaatq cagttataag aaacqttcac tgttggggat ctcatggtct cacqattagc tccggactat cqacaagtga tcqtaacaac gatgtqcaaa atataacgta cqaaqattgt tccataggaq qatcactgag tgqaattcat ttcaagacac atccagctgg tqqacacggg acagtcgatc aagttactta tcggaacatt gaaatcaaaq atgtaacaag acatqgcata gagattcaac aagattaccc gaataatgat ggtcatccgt cgaataacat caagataaac aatttgaatc tgatcaacgt cactggaaca gtcaaaggta aaacaacaag aggagtgcgg atcagctgtg gatctggatc atgcacaaac tggaaatggt ccgatgtcaa gataacaggt gcacacaaaa aagacgattg taatttccat ccagatggat attcatgttg a <210> 102 <211> 1101 <212> DNA <213> Chrysomela tremulae <400> 102 atgttgtcgt tgagattgat tgctttattg tcagctgcat caattgcagt ccaagctact ccagttgtag attctagctg cgccatcact aattttgacc aagtagcctc tgttgtcgac aaatgtaccg acattgtgat ttccaacctt gaagtaccag ctggtgaaac tctaqacctc gagttcaaga aaactggtgt caaqqtaact ttcgagggta ggactacctt cgqatataag gaatggaaaq qcccactatt qaqqataaaa ggaaaaqcaa taactgttgt aqqaqctgaa ggttcagtcc ttgatggaca aggacaactc tactgggatg gcaaaggagg aaatgggggc aaggataagc caaagttttt caaaataaag gcaacagagg gttctcattt caaaaatatc aatctgttga actgcccagt ccagtgtact tctattgacc attctggtcc actgactctc agtggatgga atatcgatgt ttctgcagga gataaaaata atttgggtca caatacagat ggattcgaca ttaacaccac agacqqattg gtaattgaaq atgcaattgt taaaaaccaa gatgactqta ttgcagtcaa ccaaqqtaca aatttcqtct tctccaattt qqaatgttac ggaggaattq qcctgagtct gtcaqttggt accagtcatq acattttgaa aaataccgtc agaaatgtca ctttcaccaa ttcagttgtt gccaaatcac gcaacggaat ccatatcaag acccacacca attcagggca aggtatcatt gaggatatta cctacagtaa cataagaatg acagatatct ggaaatatgc tgtcaatatc gaacaggact acaaacaagg aaaacccact ggacacccca ctgacaacat tcctatcaaa ggcttgacac tcaagcaggt ttctggtact ttgaccggag atgaatcagt tccggtctac atcatttgcg ccgatggagc ttgtagcaac ttcaactggt caggtgtatc attcacagga gcagcacata aaagcaattg caccatgata cctactgggt tttcttgttg a <210> 103 <211> 1071 <212> DNA <213> Chrysomela tremulae <400> 103 atgaacgcaa ttttcggttt attattagcg actgcttcga cagcctttag tttttcgctg aactgcacca tcaccgaatt ttctcaagtc gcattcgtag tgaattcctg ccgcgacatc gtaatttccg atttagtagt tccgggtggc caaatactgg agttggacct gttggcagga acgagagtga cctttgaagg cacaacaaag ttcgaatatt actattgggc aggacctttg ataagggtca ggggagaaaa tgtgtatttt agaggagccg aaggatccgt tttggatgga cagggtgcgc tatggtggga tggaaaaggt ggcgcagtcc ctggcaaacc gtacatgatc gaaattgatg tgactggcgg attgttcgaa gatattttcc ttttgaactg tcctcatcat tgcgtcatca tctccagcac tgatctcatt ttggacagat ggaccgttga tgtgtcccaa ggaaatataa aaaatctggg ccacaacacc gacggcttcg acatcatcta cggcaggaac gtcaccatca aaaactcgac ggtqtcgaat caggacqact gcgtggccat caatcggggc gagggcatgc tgatcacgca gatggactgc accggcagcc acggtctcag catctccgtg ggattcagcc atcgctcctt cttgcacaac agggtggtcg acgtcatcat cgagaacagc gtgttgagag acggagataa cgggatccac gtcaaaacgc acacggatgg ttatttgggc gagatcaaga atatcaccta cagaaatata catatgactg gaataagaaa tttcggagca gaaatccagc aqaactaccc tgacgqtact ataaaaccaq tcggcaacat cccaatcacg aatctgactt tcatcaacat cactqqaact atgacaqqaa aaaaatcgac tcccqttctg atagtatgcg cggaagacgc ctgcagggac tggaaatggt ccaacgtttc aataacccac gctgcaaaac ctagcaaatg ttcttacata ccagaaggat tcatttgttg a <210> 104 <211> 1131 <212> DNA <213> Chrysomela tremulae <400> 104 atgatgtctt tttctgaatc agttaccctt ttattattqq ttgtagtaag catatcagct actctcaacq qttatgagtc catcqattat gccacaqctq taaacctcac tqcaqactat tgctacgcga gaagttatga agaagtatca tccatcgtta aagaatgtaa atatatcgtt cttgtggact tcgaggtgcc tgctggaaaa actcttgagc tggacttgca gaagaatgct gtgttattat ttgaaggaac catcaaattt gcggtagcac actgggatgg tccattcata cacataacag gaaaaaatat ccaagtcatt ggaggaacca atcatctcat tcatqgacag ggtgaattat attgggacgg tcacqqaggc tctggtggaq tgaagaaacc tgaqaaagta ttctcgqtqa atgtaaatgg qqqaattttc aaaaatatca atatcaagaa ctqtccaacg gcttgcgtta aaatatatgg gaatqattta actttctctq gatttaatat cqatctcaat gatggattca agaataaatt ggccaaacac acagatgcga tgacattctc ccactccaac aacatcgtca ttgagaactc caaaatcgtt aatcaggatg attgtgtgaa cgttatttgc agcagcaatg cagttataag aaacgttcac tgttggggat ctcatggtct cacgattagc tccggactat cgacaagtga tcgtaacaac gatgtgcaaa atataacgta cgaagattgt tccataggag qatcactgag tggaattcat ttcaagacac atccagctgg tggacacggg acagtcgatc aagttactta tcqqaacatt gaaatcaaaq atgtaacaag acatqgcata gagattcaac aagattaccc gaataatgat ggtcatccgt cgaataacat caagataaac aatttgaatc tgatcaacgt cactggaaca gtcaaaggta aaacaacaag aggagtgcgg atcagctgtg gatctggatc atgcacaaac tggaaatggt ccgatgtcaa gataacaggt gcacacaaaa aagacgattg taatttccat ccagatggat attcatgttg a <210> 105 <211> 1098 <212> DNA <213> Gastrophysa viridula <400> 105 atgtggttta tcagactgat aggcgtgctc tcagctgcgt cgatcgcagt tacagcttcc cctgctgggg atcagagctg caccatcacg aacttcgaac aagtgtcttc agttatcgct gattgcaccg atattagggt ttctgacctt caggtacccg ctggggaaac tctcgacttg gagttcaaaa aqacaggtgt taccataacg ttcgagggga agaccacttt cgggtacaaa gaatgggccq qacctctgtt gagqataaaa gggaaagcqc ttaccgttgt tgqaqctgaa gggtcaqtac ttgatgggca aqqacaactc tattqqqatq gcaaaggagq aaaaqggaag aagaagccaa aqtttttcaa aataaaagca acagaagqtt cccatttcaa qaacatcaat ttattaaact gcccagtaca atgcacttca atcgaccatt ctggaccact tactctcagt ggatggacca tcgatgtttc tgcaggagat aaagacaact tgggacacaa cacagacgga ttcgacatca acactaccga cggactggtc atcgaaaata cgattgtcaa aaatcaagac gactgtatcg ccgtcaacca aggtaccaac ttcttgttct ccaacatgga gtgctcagga ggacacggca tqagcctgtc ggtagqcacc agtcacgaaa tatcgaaaaa caccqtacga aacatcactt tcgctgattc agtqqtagcc aaatcgaqaa acgggatcca catcaaaaca cacactaatt ccggacaagg catcatcgca gatgtcacgt acaggaacat cacgatgaca gatatctgga ggtatgccgt caacgtcgaa caggactata aagagggatc accgaccggc cacccggtcg gcaacatccc catcgagggc ctgacgttgg acaacgtccg tgggactctg acgggcgggg aatcggttcc ggtgtacatt atatgcgccg agggggcctg caccgacttc aattggtcta qcgtgacttt cgacqqagca gcgaatccga gcaactgtag cctqataccg catggctttc cttgttga <210> 106 <211> 1110 <212> DNA <213> Gastrophysa viridula <400> 106 atgttttcat tgaaagtact gacggccgtc gcagcagcgt tcgccgctgt ctcagcacta cccactgaga ttgaaacccc agttgggtca ggatgtgtca tcacaaatat cgatcaagtc agctcggtta ccagcagttg tactaatata gtggtttcca gtctaaccgt tcccqccgga aagactttqa aactacagct qaaqactgga accactttqa ctttcaaggq aaaqaccgtt tttgaagcta cccaatggca agqtqatcta atggagatca gtggagacaa qatcacagtg acaggagaat ctggacatgt gctcgatggt cagggagcaa aatattggga cggcaaaggc gacaaaggcg ttaaaaaacc aaaattcatc aggataaagg ctacaggagg atcagttttc aagggccttc acgtattgaa ttgtccaaaa cagtgtgtat ctctaaatag ctgtgatcac gtcactatcg ataattggac tgtagatgtt tctgctggag ataagaacaa tctgggccac aacaccgacg qcttcgatat ctcttcgagc acgaacttga tcatctcgaa ttccqtggtg aagaatcaaq atgattgtgt ggccattaac aaggctcaqc acgtgaccat atcqaatcta aagtgttcag ggggccacgg tctcagtttg tcagtcggta tgagcaaaac gtcagtcagc gataacaccg tcaccgacgt agcattcaaa gattgcacgg taacaaattc cgacaacggc atccacatca agacacattc tgacggcggc gcaggcgtca tcaaaaacgt ctcctacaaa aacatccatt tatcaggaat tcgaaaatac ggagtgaaca tcgaacagga ctacgaaaac ggaaagccaa ctgggacacc taaaaataac attcccataa cgggtttgac aatcagcggt gtcactgqaa caatgactgg atcqaactcc aaggcaqtqt acattttgtg cqcttccgga ggatgctcgg gatggacctg gggtgtttcc atcacgggat ctcataagga caactattgc aaaaaccaac cgtccaatgt caaatgctga <210> 107 <211> 1080 <212> DNA <213> Gastrophysa viridula <400> 107 atgtattccc tactcttgat attqttagtg tcgattccca gtttgttgcc gaqacctgtg gcggcaqccq actgtaccat caccqaattc tcccaaqttt cttcggtggt aqcttcctgc accgacatcq tcatctcaga gttqqtagta ccgggagqtq aaatattgga qttqqacttg ctgccaggta ccaaggtcac cttcgaaggc accacaaagt tcgagtacta ctattgggcg ggccctttga tcagagtgag aggagaaaat gtccatttca aagcagcccc agggtccgtg ttggatggac aaggtgctct atggtgggat ggcaaaggag gcgccgttcc tgggaaacca tacatgatag aaatcgacgt tacgggagga ttatttgaag acatattcct tctgaactgt cctcaccact qcgtcatcat atctaqcacc gatctcatat tggacagatg gaccattgat gtgtcccaaq qqaatgtagg tgaqctgggt cacaataccq acggcttcga tqtcatatac ggccataacg tgaccatcag aaattctacg gtgtcgaatc aggacgactg cgtggccatc aatcggggca agggcctgac aatcacccag atggactgca ccggcagcca cggactcagc atctccgtcg gcttcagcaa gcactccttc ctgcacaaca gcgtcgtcga cgtcgtcgta gaagacagtg tcttgagaga cggcgccaac ggcatccacg tcaagacgca cacggacgga tatgcggggg aqatcaggaa tgtcqtctat aggaacatac acatgacggg aataqaaaga tacggcatcc aaatccagca gqactaccca tctggcaacq gtaggcctgt cqqcaacatc cccatcacca acctgacatt ttcgaacatc accggaacca tgactgggag aaaatcaacc ccggttgcca taacttgcgc agaacacgcc tgcgccaatt ggaactggtc cggcgtattc atcaccggtg ctgccaaacc tagcaattgc acttacgtgc cagaaggatt cgactgttga <210> 108 <211> 1107 <212> DNA <213> Gastrophysa viridula <400> 108 atgcattqta cagctattgt cctqattttt ggtgctctaq tagctcttgt aqqctcggca ccaactcata actgtaccat caqaaaattc tcagaggtac agtcagttat aaatqgctgt acggatattc tggtgtcaaa tttgacaatg ccagctggca ttgttttgga gctcgatttg ctgaacggaa caaccatgac attcgaaggt accactctgt ttgaatacac accatggtct ggtccattga taagagtcaa aggagttgat gtgacgctac aaggacaacc aggttctgtt ttcgacggcc aaggaccatt gtactgggat gggaagggag ataaaggacc caagaagccc caattcatga aaatagaagt tttcgqtaga tcaattttca agaacataaa gttqctaaac tgtccacatc actgtgttta tattqqaaaa tcagatqqac ttaccataac aaactggaca atagacaatt cagcaggaga tctgcataac ttcaccggtc acaatacgga tggcttcgat atttcagcag ctaagaatct catcatcgag aacaccgcgg ttatcaacca ggatgactgc atagccattc gatacgggtc aaatatcctc gtccggaata tgtattgctc gggaggacat ggcctcagtt tgtcggtggg tttcaacaag acctcctatc ccgaaaacgt agtggaaaat gtcgttatag aqgacagcct gctcqtcaac tctgcgaacq gtatccacgt caaaacccac acagatgqct acttcggact catcaagaac gtcacctata ggaatataga aatqcgaggt atcaccaatt acggcatcaa cgtgcagcaa gattactgcg atggacgcgg tacaggaaca gcaaaatcta acgtaccgat tgatagtttg aacattatca atgtcactgg aactgctgcg aaagggtcga agctcactgg tgtttacatt ttttgtgcag aaggttcatg cagaaattgg acttgggatg atgtcaagat cacaggagca aaaataggag atttttgtac attcatccct tcgggatatg aatgtcataa agtttaa <210> 109 <211> 1125 <212> DNA <213> Gastrophysa viridula <400> 109 atgacttctc atctgatccc tctattgagt atactagtat tttcagttgc tgtgacagcg acttcgactg atagttatga acatattgat tatgaaaaag ccaaaaatct cactgcagac tattgctatg cgagaagtta cgctgaggta gcaaacgttg ttgaaaaatg tagatacatt gtcctggtga acttcgaagt tcctgctggt aaaaccttgc agctcaactt gcaaaagaat tctgtgctcc tqattgaagg gacaatcaca tttgctgtgq cacactggga tggtcccctc atgtccataa acggaaaaaa tcttcaagtc cttggaqqaa cgaatcacct cattcatgga cagggtgaaa aatactggga tggtagaggt ggcggtggtg ttcagaaacc acagagagtt atgtcgatca atgtccaggg tggatctttc aagaatatca acataaaaaa ttgtcccatg ttttgcgttt ccatcatagg aagtgacctc acattctctg gattcaatat cgataataat gatggtttca aaggtggatt agccgtgaat actgatgcga tgggattcgt ctactccaac aacatcctaa ttgagaactc aaaaataatc aaccaagatq actgtatcaa cgtaatggcg agtagcaatq ccgttatcag gaacqtccac tgctggqqat ctcatggctt qaqtttcagc tctggccttt cgaaaactga cgaaaacaac gacatccata acgttacctt ccaagactgt tccataggag gttccttgaa tggtatccac atcttgacga tgccatctgg aggaaaagga aaaatcagcc aagtgactta cagaaatatt caaatcatag atgtaaccca aagggcaatc gaatttcgtc aagattactt ggataatggt catccgaaaa ataatatcgt tatcgatggc ttgactctgg acaacatcta cggtactgtt acgggtaatq cagtggcagt ttatatcaac tgtggatctq qatcatgcaa aaattqgaac tggtcaqqaq tcaagataac tqqaqccaag acgaaagact attgcaattt cgtqcctcat ggctattcct gctaa <210> 110 <211> 1119 <212> DNA <213> Gastrophysa viridula <400> 110 atgttcatga aatatttcgt tacattttca gtaattgtgg ttggaatatc agctacaacc attgaatatg actacgagac ggccaaaaat ctaacctcag attattgtta catcaagagt tataacgaag cqccagctat tgttaaaaaa tgtacccata tcgtcgtaac ggqttttgaa gttcctqctq qaaaaactct aqaactcaac ctgctqaaqc aatctgtgct attqttcgaa ggaaccctca catttggagt ggcacactgg gatggtccat ttatccatat cacgggacaa aatattcaag tcataggtgg aaccaatcat gtcatccatg gacacggaga gaaatattgg gatggacaag gagacagtgg tggcgtgaaa aaacctcgta gagtactctc agtacaagtg cagaaggcaa ttttcaaaaa tatcaatatc aagaactgtc cattgttttg tgctacagct ttgggacatg atatcacttt tactqqcttc aatatcgata acaatgatgg attcaagaat ggcgttqcaa qqaatacaga cqqtatatcg ttcgctcaat gtagtaacat aatcqtcaag gactcgaaag tctacaacca ggatgactgt gtgaacattt tgggcggctg caagaatgtg tacgtcgaaa atgtccattg ctggggatct cacggcctct ctattacttc tggaatggca acaacagcag ataaaaatga catatataat gtcacctatc aagattgttc cattggagga tcgtatgctg gaattcacgt caagactacc caaccctgtc ttggacctgg cacgataagg aaaattacct acaagaacat ccaqataaca gatgtaataa gagatgcaat cgaqattcaa caagattatc cqaagaatga tqqtaactcg gcgaacaaaa tggcgatgac tqaaqtgaac ctgatcgatq ttactggatc agttactgga tcagcaaqaa gagttcgcat caactgtgga tctggatctt gctccaactt cagatggtct aacgtcaaga ttactggttc aaaaactgat gatagttgta attatatacc aagtggatat tcatgttga <210> 111 <211> 1125 <212> DNA <213> Gastrophysa viridula <400> 111 atgtatatgt qcataaaatc ttcaatttta ttttcaataq tgatagttgg aatatcagct gaaactatcq attatgatta cqaqacggcc aaaaatctaa cctcagatta ttqttacatc aagagttata acgaagcgcc agctattgtt aaaaaatgta cccatatcgt cgtaacgggt tttgaagttc ctgctggaaa aactctagaa ctcaacctgc tgcagcattc tgttctgttg ttcgaaggaa ccctcacatt tggagtggca cactgggctg gtccattcat ctacatcacg ggacaaaata ttcaagtcat aggtggaact aatcatctta tccatggagg gggaqaaaaa tattgggatg qacttggagg cagtqqggtg aagaaaccaq aaagagtgtt cttcatgaat gctcaaqtqq caactgtcaa aaatatcaac atcaaqaact gtccactgtt ctqtqtttcg gtcttaggac atgatctcac cttctctggt atcaatattg ataacaccga tggattcgcg aatggactgg caagaaatac cgatggtata acgttcgctc aatgtaaaaa cgtagtacta gagaactcca agatcataaa ccaggacgac ggtgtgaaca ttttgggcgg ctgcagcaat gtgtacgtca gaaatgtcca ttgctgggga tctcacggtc tttcagtcac ttctqgaatg gccacaactq cqgctaaaaa cgacqtatat aatgtcactt tcgaagattg ttccaatgga ggatcatttq ttggaatcca tqttaagact actcaqcctt gtcttggacc tqqatcattc aaacaaatca cttacaagaa catccagatt tcagaagtca caagaagtgc catcqagata caacaagatt acccaaagaa cgatggtaac tctgcaaaca caatgaaaat aatcggagtg gatcttatca acgttactgg gtcggttaaa actacctcgg caaggaaagt gcgcatcaac tgtggatctg gatcttgctc caacttcaaa tggtctaacg tcaagattac tggttcaaca actgcagata gttgtaatta tcatccaagc ggatattcat gttag <210> 112 <211> 1101 <212> DNA <213> Leptinotarsa decemlineata <400> 112 atgtgtgaca ctttaaaatt tttcggaatc ttaggattct tggtgctcac ttctgctcag cctagtctga atcaaagtgc ctcttgcacg atatcgaaat tttctgacgt gtctgcggtt ctcagtagat gtactgatat tgtcgtttcc aatctgacag tgcccggcgg aaaaatactg gacctggatc ttcagagtgg ctccactgtt acattcgaag gaactaccaa gtttqaacat gctctctggg caggaccttt agtcaqagtt aaaggagaqa aagttttagt acaaqgagca acaggctctq ttcttgatgg tcaaqqagct ctttqqtqqq acggaaaggq aqqatcggtt gcaggcaagc cttacttttt cgaaatagat gtatcaggag gatcactatt caaaaatgtt ttcttgctga attgtcctca tcattgcgtc atcatcgcaa gttctgacac aactctgact ggatggacca tagataattc tgatggagat aaacaccagc tgggacacaa cactgacggt ttcgacatca tcaacggcca aaacatcgtc atcaccaact ccaccgtgaa gaatcaagac gactgcgtgq cqatcaaccg cggttcaaac atgcttatct caaacttgca ctqctccgga tctcacqqtc tcagtttgtc cqtcqqcttc agcaaacact ctttctccca taacaaagtt accaacgtca cattcagaga ctctqtgatt gtgaatgctq gttctggaat tcacqtgaag acgcatactg atggtgggct cggggagatc agaaacgtga cttatgagaa tatctatatg gcagggataa ccaactctgg gatcaacatt cagcaggact acgcaaacgg aggtagcacg ggaattctta ataataatat tcctataacc tctttgacgc tcaccaacgt gacgggacac gttgtaggca aaaaagcttt aagagtcaag atttattgtg ccaatgaagg ctgcttcgat tggaaatggt ctggagttga agttctgggt ggaagacaga aaaaccagtg tacttatgaa ccagagggat tttcctgttg a <210> 113 <211> 1086 <212> DNA <213> Leptinotarsa decemlineata <400> 113 atgttttccg taaaagttat aagtttgctc gtcacttgct gggcactttt ttccataact gatgcaagtg ctgtaggaga agattgtacc atcacaaatt tctctcaagt ctccagtgta gtgcagagat qtaacaatat agttqttaga agtttgaacq tacctgccaa gcaacgttta cacctaaacc tqaaaagggg aaqttctttg acattctcaq gcattactaq atttqgagtg gctgaatggg atggccctct tgtcataatt cagggcgaca atctgaaagt gaacggtgaa ctaggttcga aattagatgg gcagggtgct gaatattggg atggtaaagg agggagtgga tctaaaaaac cagttctcat tcaaattatt ggaagcggag atttcaacaa catccattta ctgaattgtc ctgaacgatg tgcctctgtt cttggatcgc atctctcact ggttqgctgg aatatcgatq tctctgctgg agataaaaac aacttgggtc acaataccga tgqtttcgac gttgtgqqaa ctgatataac aataaqaaat tccgttqtqa aaaatcaaga tqactgttta gttgtgaatc qtggaagtga catqcatttt caaaatatct actgctctgg tqqacacggg ctcagtctgt ccattggtat gagtaagacg tcatataacg ataatgtggc tcgcaatatc actttcaaag actgcaccgt tgtcaattcc gataacgcaa tccacatcaa aacgcacaaa gatggcgccc aaggactcat tcaagacgtt acttataaaa atattactct atacaatata agaaattatg gtatgcaaat tcaacaagac tatcccaatc acgatggacg accagttgga aatgttccaa ttagaggcct gataataaca gatttcaaaq gcaccatgac tggaaagaat tcagtaccqq tagaagtcgt ttqcqcagca ggagcatqtt ctgattggat ttqqtcagag attaaaatct caggaaatag caagccttgt tctatgaact attatccatc tgggttttcc tgttag <210> 114 <211> 1098 <212> DNA <213> Leptinotarsa decemlineata <400> 114 atgatttttc ttactgaaat tatatttgta actacccttt tgtcgggatc attttcattg gcttcttcac cqggttgcac tatcaqaagg tattcaqatq tgccatcagt cctcagtaac tgcaccacaa ttataattga caatctcgta tttcctgctg gtattacgtt tgaactctat ttacaaaaag gaactacagt gactttcaaa ggtactacca ggttcgagta cacaccgtgg tctggtcctc tcatcagagt caaagggagc aacattacta ttcaaggaga gccaggttca gttcttgatg gacaaggtcc actatactgg gatggaaaag gagacaaggg accaaaaaaa ccccagttca ttaaaataga agctaatgaa agatctgtaa tgaaaaatat caatatactg aattgtccac atcattgcat ctacqttgga aaatctqatq ggcttactat aaqcqactgg gtgattgatq tqtcagatgg agataaaaac aatttcaccq gtcataacac tqacqgattt gacgtttctg gagcaaataa tctcatcatc gaaaacacta gggtcatcaa tcaagacgac tgtatagcta tcagatatgg taccaacatc accgtcagaa atatgtattg ttccggagga cacggtttga gtttgtcggt aggattcaac aagacgtctt atcctgaaaa tgttgtttcc aatgttctta tcgaagacag taccgttgtc agatcagcta acgccataca tatcaaaact cacactgacg qatggttcgg tctcatcaat aacatcacct acagaaatat tgaqttgata ggaataacca attttggaat taacatccag caagactatq caaatggaga aqqtacagga actgttagag acaacattcc aataagaaac attaatttat ccaacataca tggaaatatg aaaggtcgaa aaacaagagc attttacatt tattgtgcaa aagatgcttg ctctgactgg aaatgggttg atatgaaaat tgaaaatgct tcgaagtcga actcctgtaa ttactcaccg cctgattatc cttgttaa <210> 115 <211> 1086 <212> DNA <213> Leptinotarsa decemlineata <400> 115 atgttttttg cgaaggtgat tagcatactg agtattcatt cagcaatctc tattacagca tggggaagtg ctataagtgg aggctgcacc attactgaat tttctcaagt gtcccaagta gtgaacagct gtacgaatat agtggtgaaa aatttgaatg ttcctgctca gcaaacactg catatgaaac tqaaaaaaga aagtactctg tcatttgaag gtaccactat ctttqgagta gccaattggq aaggtcctct cattaaaatt gaaggcgaca ctgtcaaaat agatqgagca gaaggttcaa aatttgatgg acaaqqagct gaatactqqq atggcaaagq aqqtcaaggt tctaagaaqc cactcttctt cacatttcag ggaagtgqaq aattgaccaa catcaattta ctcaactgcc cagaacgttg ttctgatgtt catggatctc atattaccat cgatcattgg aatatcgatg tttctgctgg agataagaat aacttaggcc acaacactga tggaatcggc gtatggggag aaaacataat cgtcaagaac agcgttgtga ggaatcaaga cgactgtttt gttatggctc gtggacggga catacatgtc aacaacctat actgttcagg aggccacggg atcagtatgt cqgtgggtct gagcaagact tcctttgaaa gcaatttcgt tcaaaatgtc accttcaaaq acatcaccct tgttqattcg gacaatqqaa ttcacctcaa aacacacaaa gacggtggtc cgggacttgt ggaacatatt acctataaaa atatcacctt gaaacgtatt agacgatatg gtatagaaat ccaacaggat tatccagaaa atgatggaaa accaacagac aatattccaa taagagacgt gacggtgatt ggagtgaggg gcactatgac tggtaaacat tctgttccag ttagggtttc gtgtagtact ggagcttgtg ctaactggaa ctggtcagag gttgatatta aaggaaacca taagccctgt tctatgaatt ttaaacctaa cggqttctct tgttga <210> 116 <211> 1086 <212> DNA <213> Leptinotarsa decemlineata <400> 116 atgttttttg caagagttat aaatttgctc gtcactggct gggcactttt ctccataact aatgcaagtg ttataggaga agattgtacc atcacgaatt tttctgaagt ctccagtgta gtgcagagat qtaacaatat agttqttaga agtttgaacq ttcctgccaa aaaatcttta cacctaaqcc tqaaaaaggg aaqttctttg acattctccq gcatcactac ctttqgagta gctgaatgqq qtggccctct tgtcqtaatt cagggcgata atctgagagt qaacqgtgaa ctaggttcga aattagatgg acagggtgct aaatattggg atggtaaggg agggaaagga tctacaaaac cagttctcat tcagattaag ggaagcggag atttcaacaa catccattta ctgaattgtc ctgaacgatg tgcctctgtt catggatcac atctctcact ggttggctgg aatatcgatg tctctgctgg agataaaaac aaattgggtc acaacaccga tggtttcgat gtttcgggaa ctgatataac agtgaqaaat tccgttgtga aaaatcaaga tgactgttta gttgtgaatc atggaaataa catqcatttt caaaatctct actgctctgg tqqacacggg cttagtctgt ccattggtat gagtaagacg tcatataacg ataatgtggc tcgcaatatc actttcaaag actgcaccgt tgtcaattcc gataacggaa tccacatcaa aacgcacaaa gacggcaccg aaggactcat tcaagatgtc acttataaaa atattactct ccacgatata agaaattatg gtttggaaat tcaacaagac tatcctaatc acgatggaca cccagttgga aatgttccaa ttaggggcct gacaataaca gatgtgaaaq gcaccatgac tggaaagaag tcagtaccqq tagaagtcgt ttqcqcagca ggagcatqtt ctgattggat ttqqtcagag attaaaatat caggaaataa aaagccttgt tctatgaact atcatccatc tggatttttc tgttag <210> 117 <211> 1068 <212> DNA <213> Leptinotarsa decemlineata <400> 117 atgtacgctt tqgtggtatt cacqacattg gttgcttcca tcctcagttt tacaagctcg aaaaattqta caattcacaa ttttqaagaa gtgtcqtccq ttttacaaca ctqttcagaa ataactgttt tcaatgtcac cgttcctgct gggaacacac tggaattcaa tttqaagaag aactcagtcg tgactcttga aggtaccatc actttccagt ttcaggcttg gaaaggacct ttattagaat tcaaaggaga cggaatcacg gttaaaggaa caaaaggctc tgtgattgat ggaaaaggag aactctactg ggatcatttg ggcgataaag gattaactaa accacaatta gttaacatag tcgcttctaa tgattcagta ttccagaatc ttcatttacg gaactgccca aatcattgtt tttacctcag aaataqtgac aacgttacta tatctgaaat agatattgat gtatctgatq qcaataagaa taattttaca ggacacaata ctgatggttt caaccttggt ttcgtgacca acacgatcat aaaaaattgc actgtgaaaa accaagacga ctgtgtatct attaatgcag cgtcaaatat ctcaatcagg aatatggatt gctccggtgg ccatgggttg agtatttcgg tgaccaggaa tccagtggat aatgttacgt acagcgatag tattgtgaga aattcagcca atggactaca cgttaaaacg cacactaatg gcggagaaac tgtaattagt aatattacgt ataaaaacat caaattggaa ggtataacta actttggtat aaacatacaa gaggactaca aagatggaga aggtacaggc attccagcaa ataacatcaa aattgttaat ttgatcatat caaacatcac tggaactatg aacggtaaag actcgaaacc ggtgcaaata atttgtgggg atactggatg tgcaaattgg caatggtctg acatctatat cgtaggcaat tcgactccaa gctattgtaa ctatgcacct tccggattta actgctga <210> 118 <211> 1083 <212> DNA <213> Leptinotarsa decemlineata <400> 118 atgcttgttg ctgacttgat tagtatttta atctcatttt ggggggtatc ttccatggtg gtaacgcctq tagctgggag caqctqtacc atcaccgact actctgaagt ttcaaaggta gtgagcagct gtacaaaaat agttttgaag agttttgagg tacctgctgg caaaacacta aatctaaact tgcaaccaga aacttttcta attatcgacg gtactatcaa atttgggaaa tctaactggg ctggacccct ggtgaagatt caaggacgtc gaataaaagt atcaggaaca ccaggttctg tatttgacgg acaaggagca gaatattggg atggaaaagg cagtcaagga acgaaaaaac cqgtgttact cagagtaaat gcacatgaaa gtgaatttta caacatcaat ctattgaatt qtcctctacg ctqtqcttct attggaqqat ctgatatttt cttqaatggc tggactattg atgtctctga agcaggtaat cttggagtta atacagatgg atttgatgta tctgggcaca acatgacaat cataaactcc cgagtcttca atcaagatga ctgtgtagtg gtgaaccgtg gttctgacat gttttttcaa aatatctatt gcaaaggagg ccatggactc agcgtcgcag tgggaattga tcctacggta tacgagaata atgtagtatc caatattacc taccaagatt qcattgttga aaattcgatc aacgggattc acgtgaaaac tgttaaaggc ggtggacctq qtttgatcag taatqtcaac tataatcqta ttagaatgat qaatatcaaa gagtttgcga taagaataca ccaggattat ccatctaacg ataaaaaacc cgtaggaaat gtaccaattg acgatttgaa aatatctcac gttgaaggca atatgaaagg acccagatct tccgtagtcg aagttctttg tgcttctgga gcatgctcta attggaagtg gtccgatatt tctgtaacag gaaataagct gccttgttcc atgaactata agccttctgg attctcttgt t ga <210> 119 <211> 1089 <212> DNA <213> Leptinotarsa decemlineata <400> 119 atgtattttg cgagtttagt ggctatttta gcgatatgtt gcggactttt ctacactgtg gatactaata tactgaagag agattgtgat atcaccgact tttctcaagt accagacgtg gtgagtagat gtaccaatat aagagtatgg aaaatggatg ttcctgctgg cgagactttg aatctgaaac tacaaacagg aactacttta tatttcaagg atatcgttaa atttggggta gctaactggg acggtcctct agttaatatt gaaggaaatq atttgagaat atatqgagct gcaggtatcq qattctccgg acaaqqagaa gaatattqqq acggaaaagg taqtcaaggg tctaaaaaac cactcttttt gaaaatcaac gtacagggag gtgaattcta tgtcttaaat cttgtgaatt gtccagaacg ttgtacctcc attactggat cggacatcct catcaatggc tggactatta atacttctga tggagacaaa gataaccttg gcgttgacac tgtcggtttt gaggtgtctg gtgataacat caccatcaga aactcgctag tattcaatca ggatgactgt attagggtgg aqcgtggttc gaatatgtta atcagtaaca attattgcaa tggaqgtaac ggtctaaacq tagaagtggg gqctqatcct gaagcatatq aagaaaatgt qqtatccaat atcacgttcg attatggtgt tattgaaaac tcaacttacg gtatccatgt taaaacagtt aaaggaagtg gacctggttt attacaaaac gtctccttca gcagcatcaa tatgattgga atcaaaaatt actcaataag aatacaccaa gattatcctg ctgatgacaa caaacctgaa ggaaatgtac ctatcagagt cttgaatttg aaatatgtga ccggtaatat gagtggaagt tcgtctgtac cagttgatgt tgtttqtgct tccggagcgt gctctgattg gacqtggtct aacatatcta taaaaggaaa taqcacgcct tgttccatqa actatgaacc ttctqggttc ttttgttga <210> 120 <211> 1119 <212> DNA <213> Leptinotarsa decemlineata <400> 120 atgctacttt tgggcggatt agtattcttg gtagctgttg tagctaattc agcttcaccg actaatctca ctgatgttgg tgctagttgt accattacga aatttaatca agtttcttcg gtactaaaca qttgtacgaa tgttqttgtg aaaaacctcq ttgtcgatgc cgqacaaact cttaaacttt ttctgaaggc aqqaqcgact ctaacttttq aaggaacaac tacatttgca tatgctgaat ggaaaggacc cttgatttgg ataaaaggaa atggaattac agtggttgga gctcaaggtc acaaactcga tggccgagga gagaaatggt gggatggcaa aggagatggc ggaaaggtga agccacaatt catgtttatc caagccacag gaggatctgt tttgaaaaat atcaatatgt acaattgtcc ccatcaatgt gtgggaatct ctgatagtga tcacqttact atcagcaatt qqcaacttga tagttcggct ggcaacccaq tgaatgggaa agaaqttggg cataacaccq atggatttga tctttacaag tccaattaca tccttcttga tqqcattgtt gttagaaatc aagatgattg catttgtatc aatggaggtt ctcatctgac attcagaaat ttatggtgtt acggaggtca cggcttgagt atatcatcag gaatgagcat gacggattac aatctgaatg tcgtttccga tgtgcatttc gaacattgcc atgtgagcga ttcacgaaat ggaattcaca tcaaaacaat agcagacgga ggaaaaggaa agatgtctgg cctttacttc agcgatattc aattgtcagg aatatctacc tacggaatca acgtcgaaca aaactataga aacaatqqtq qaccttctgg aaaqccaaac aataatqtqc ctatcgatac tctqqagatt aatggggtqa ctggaacttt aaatqqgccg tggtcagtqa aagcctatat tctatgtgcc tctggagcat gtaaaaattt caaatggtca aatatcaacc ttcagggtaa ctcacggccc gactcctgca acttccatcc ctccggatat aattgttag <210> 121 <211> 1101 <212> DNA <213> Leptinotarsa decemlineata <400> 121 atgtctctcc taagcgtgat aattqcgtct ctattagtqa ctctcacagc agccaagccg tcttttactq qqgacaattg cacaataact gagttttctc aagtcgaatc tqtttcagcg aaatgtacca acatcattgt gtctaatctg caagttccag gtggtgaaac gctagatttt aaattcaaga agccaggagt tcatattact tttgaaggaa aaaccacgtt tgggtaccaa ttatggaaag gaccccttat caggattcag ggcgtaggaa ttaccgttac aggagcacct ggttctgtac ttgatggaca aggtgctctt tattgggagg gcaaaaacgg aaaaaagact aaacctaaat ttttcaaaat aaaqqttaaa gaaggatcaq tgttcaagga tatacactta ctgaattqcc ctgttcattg tqtatccgta agtatqtccq accacgtaac cctatcagga tggaacatcg atgtttctgc aggagacaag gatgagctag gacacaatac cgatggattc gacatctctg aaactaacaa tatactaatc gaaaacgcaa ttgtccagaa tcaggacgat tgtgtcgccg taaatcaagg tttcaacatg acattcagga atttggagtg tattggaggt cacggactca gtctttctgt cggtatgagc cacgaggtga tcaaaaatac ggtgqctaac gtcactttta qagactcgat agtcaaaaat tcgagaaacq gtattcacgt taaaactcat actaactctq qagaaggact catcaqggac ataacataca aaaacattga qatqacagga atctggagat acggagtaaa tgtaqaacaa gactacaaqa acggaaaatc cactqgaaat gcaacaaata acatccctat caggggacta acattgacta acgttaacgg acagttaaca ggaccagagt cagtaccggt ttacatactt tgtggatctg aaggatgtga aaacttcgaa tggtccgaag tttcgttccg tggtgcttca aatccaagtt cttgtaatta tgaacctcgc ggttttcatt gcccaaaata a <210> 122 <211> 1098 <212> DNA <213> Sitophilus oryzae <400> 122 atgaacgcac tgctttacaa catcatattt cttgtttctt tggcattagc caaaccttca aacaactcct taggggccac ttgtacagta agttcatacg acgatgtggc atctgcgatt tcaagttgca ccaccatcaa cctgggctct tttacagtgc cggccggtac aactttagct ttaaaattaa aaaccggcac cactgtcaat atccaaggta ctatcaaatt cggataccaa gaatggaaag qacctttggt tcaaqtgtcg ggaaaacaaq ttacaattaa tgqtqctagt ggaaaatttq atggagaagg tqctaactac tgggacaqtc aaggtgacaa aqqaaaaaca aagccaaagt ttttcaaaat atctactaca tcaagttcta ctttcaatga tattactctg aaaaattgtc ctcatcaatg cgtctctatt agcagcgctt cagatactac tttatcaggt tggaacattg acgtatcagc aggagatagc gcaggtggac acaacactga cggcttcgat atctccggct cqaccggcat caccattaaa aactccgttg tcaagaacca agacqattgc gtggctatta accaaggttc aaacttggtt ttcgaaagct taacatgcag cgqtqgacac gggctcaqtc tctcagtagg acaaaqtact gagaatqqqq atgcaaacac aqttaaaaac gtcgttttta qcgattgtac cgtaaagaat tctcgaaatq gcatccacat aaaqacgcac aaagatgccg gtaccggtgc aatcagtgat gtgacttaca aaaacattaa actttctgga attaccaatt atggcattaa cgttcaagag aattatgcaa atggtggcga tagtggtgat ccgttaggta atatacctat tacaaatttg aatctccaat cggtgacagg ctccatgtct ggaggatcta gcagcatgtc ggtatacatt ttatgcggcg atggaggatg cagtaattgg gcatggtccg qtgtttctat ttccaqcgcg aaaaaatcaa acagctgtaa ctttacgccc tctggattca cttgttaa <210> 123 <211> 1089 <212> DNA <213> Sitophilus oryzae <400> 123 atgattatga attttacaat cacattttta cttttagcaa atttagcaat tgcggtacta tcaagtccag tqttaaatgc gacctgtact atatcttcat tcgatgaagt tgctaacgtt gttgccagtt qttctgacat tgttctggaa tcttttacaq ttccagctgg aaccacactt aacatgaatt tqaattccgg ttctactgtg actgttaaaq gtgtaattaa qtttqgatat caagaatggg ctggaccgct ggttcaaatt tatggaaacc aaatcacttt tagaggagca ggtggatatt ttgacggtga aggtgcaaac tactgggacg gagacaacaa aaatggaaaa gtgaagccca aatttttcaa aatcaaaact aaagctaatt cgatttttag cgacattact cttaaaaatt gtcctaagaa ttgtgtttca atctcggctt ctgacactac cttgactgat tggttagttq actgttccga aggaqattcg aaaaaaggaa aaaacacgga tgqatttgat ttgtccaqct ctgccgatat cacaatcaga gattccqttq tgatgaatca aqacqattgt gtagcaatta atcaaggatc gaatttctta tttgagaqqt taacatgtag cqqtqgtcat ggactcagtc tttccgtcgg tctaagtaaa tctaatggtg aggtaaacac agtccggaat gctactttta ccgattgcgt cgtcaaaaat tctaaaaatg gaatgcatat taaaactcac aacgatggag gtactggggc cattactgac atcacttata gaaacattaa actttctgga atagaaaatt atggtattga gattcaagag aactacccca atggaggcga tccagtaggc aacgtaccta taacaaatct aaatttagaa tcagtgagcq gaaatatgtc tggaqcaaag aatagcatqq ctgtgtatat tttatqtgcg gatgaaqqct gcaataattg qtcctggtca aatgtcgcta ttagtaactc aaagaaagca aatagttgta actttactcc aaatggattt tcctgttga <210> 124 <211> 1095 <212> DNA <213> Sitophilus oryzae <400> 124 atgaagatat ttggtgtgtt tttaqttgtt tctgttgccc tagtctattc agaaqtttgt acagttctcq aattcagtaa cqtccaaaat gcagtccaaa cctgcaacga tatcctctta gaaaacgtta cggtccctgg cggagaaact ttgaagcttc acctccaaga tggcacaagg gttactttta ggggaatcac aaagtttggt tatgcacttt gggaaggccc tctaatcgaa atcaacggta ctaacattac agtagcagga gaagatggat ctatctttga tggacaaggc caactatact gqgacggcca aggagaatgg ggagtagtta agcctaaatt tttcaccatt caacttcata attctactat gaaaaatatt tacgtattqa acacaccggt tcattgtgtt ttgctaaccq actcgtcaaa tqtaqagcta agtaattqqa ccatagatga ttctqctgga gataaggatq tagctcctga aaaqtatggt cataacacaq acggatttga cqtatttaat tctacaaata ttgttgttaa agatgctttt gtttataatc aagatgactg cgttgctgtg cgaagtggga acaacattac tatcgagaat ttctattgcc acggtgggca cggactcagc atctcagcag gctggagtaa cgactcattt ttcatcaata ccttgacaaa tgtaatcatc agaaattcac aattggtagg aggccgaaat gggatacaca taaaaacaca catcgatgca ggaaaggggt tqattgccaa cgtcacttat gaaaatatta ctttcagtga gatqqagtac tatggtataa atattcaaca gaattacaag aatctcccqq aacaaaatac aaqttatccg gtcgatcctg acaataatat acccataaaa aatctggaat tgaagaatat tcaaggatcg gtacagtcca acgctatacc agtgtacatt ttgtgtgccg atgaaggatg ctttgactgg aattttgtta atgttaccgt agaaggaact aaggataata gttgtaatta tgtacctagt aattatactt gttaa <210> 125 <211> 1083 <212> DNA <213> Sitophilus oryzae <400> 125 atgaattttc gaattgttgt gatagtgttt actttaatag gaacaaatct gcctgttaaa gctgaagata tttgtaatgt tactgcctac gaagacatac aaaatgcagt aagtaactgt agtacaattg ttttgaaaga tgtggaaatt ccagcaaata caacactaga attgcatctg aacaataata cagagttgat atttgatggt agattaactc atgtacctgt ggatttggtt gggtatttgq taacaattta tggqaatggt cttacaatca caggaacaaa agatcatgta cttgatqqaa tqggccctca acactqgaat ggtaaaaacq aaaccataat tatqaggccg aatttgttqa tqtttaaagt aaccaacact acaattaaqa atctgaatat actqaactgt gccaatcatt gtactcatat ttctgattca tctgatgtca ttgtttctaa catctttatc gacaatcggg aaggttatcc cggtgtggca ccggtgggaa aatttgcagc taacacggat ggaataggca tatcaggatc cactgagata tatatttcag atgtcgtggt gttcaatcag gacgattgcg tagctattac tgaaggacag aatatatttg tcgagaattt gttctgtaac ggatcccatg qactcagcct ctctgttggt ggaggtgatq tctcaaacgt gatcttcaaa gattccatat taactcaacc caqacaagca attcacttaa agactcacaa cqatqggact gatggtcaca tttacaatat cctctatgaa aacatcctct ttttagatgc ttttaaaatg ggaatatcca tacaacagaa ctaccctagt ggtgaactaa gaggcaacgt acctataacg aatctaactc ttctgaatgt ccatggtact gttttgagtg atgcaatacc cgtttcaata ctctgtgctg aaggggcatg ctcagattgg aactggataa atgttactgt atcagggact cagagtaata atgaatgcga gaatattccc gaaaatatgc gcgataaaat tgtttgttcg taa <210> 126 <211> 1224 <212> DNA <213> Callosobruchus maculatus <400> 126 atgactctag gtggtattgc gggtattttc ctagctgtag tttcactgac tgctagtgca acagtataca atgtgacgga ttttggggca gattctacgg gaaaaaatgc tagtaccact gcaattacca aagctattga taaqqcatct gaagactatq ggggtgttgt ccqttttacc aaaggacaat atcttagtgg accctttgag ttgaaqtcqa acatcacact acaqtttgat gatgatgtaq tqctgaagtt tctqqatgat cccaaagaat accccccttt aaccaagcta ctgccaaatg gactaacatt ctctttacca tacactcctt tgattcgagc tttcattgtg agaaacgtag tcataagagg caaagccatc ctggacggta atggagcacc atggtggaag agactgcctc ctcccaatgc taggccacag ttcctgttct tttatcaaag ctacaacata acggtggagg atgttgctat caggaacagc ccaatgtaca acatccaact catttatact gaccacgtac ttctcaaagg tatctcaata aagaatcctc ccagttacgt ggacccaggc ccgaacacaq acggcatcaa ctqcaatccc tgcaggtatc ttcacgtcac caatqtgaca gttgacactg gggacgattg cgtagctttg gacgcggatc tcggtgacaa actgatgggc agatggccca cggaacatat cctcattgag aacagtcaca tgtttgctgg acacggagct gtcagcattg gaagcgcgac caccgctgga ctcaggaaca ttactgtcag aaattgtatc ttcaacggca ctgatagggg cttgtacatc aagactcgca gaggtcgcgg aggtctggtt gaagacatcc actaccacaa catcaccatg ttgaacataa ggaaggaggg ggtcqccatt gcagccgttt acaatggtac tqacqagggt atgcacqaaa ggaacatcca ttqqqtacca atcgacgata ccacgccatt cataaggaac attcggtaca gagggatcag gggaaactct aagcttgagt ccttgttcat cgtgggattg cctgagagac aagtcctcga cgttatcttg gacgatttcc atgtccagtc tgagaaaggg atatttttga atcaaacgca gcatattatt atcaacggga aqgaacaact gtag <210> 127 <211> 1230 <212> DNA <213> Callosobruchus maculatus <400> 127 atgatgatqa ttttatggag tatcatctgc ctttctttca gtctcagcta catacaatgc aactccgaaa tatataacgt gatagaattt cgtgttgacc cgactgggca tattgtcagc actaagggta tcactgcagc aataagagaa gcatctaaaa acaatggagg tgttgtccat tttccgaaag gaatataccg tactgggcct atccagctga ggtctcatgt cacactgcaa attgacaatg gggctttgct gctgtttgag gacaaccaaa atctatatcc tcctgtcaac gttacactgc ccagtggaga ggttattgcc ctttcatata ctccactcat aagtqcattt ggtcaaagqa atatatctat tacaqqtaga ggaatgctcq acggaagtgg tqctttttgg ataaaactct tacccccacc atcaactagg ccatttttct tatactttgt tgagtctcag gaggttttgc ttgaaggtgt tcatatcaag aatagcccaa tgtacaatgt tcatttcaag gatacaagtc atattaccat caaaggaatc acaataacaa acccagaaaa caccgttgac ccaggaccaa acacagacgg tatcaactgt gacccgtgta gatacctaca tgtcagcaat gtgaccatca qcacaggaga cgatqcaatt gtgatgaaaq ctgacatgag gggcaggaca tcgaagcaqc ttaagcctac tqaacacgtc ctcattqaaa acagcttcat ttttqtaggt catgcaggaa ttagtatggg cagtgctact gctggaggat tgcggaacat cacagttaga aattgtcttt tcaatggtac aaaccgaggc atctacatca aaactgcccg tgacagaggt ggcaaagtgg aagatattca ctaccataac ataaccatgc tgaacataag aaaagaaggc gtagctatag ctgacgtcta caatggaact gatgaagggc tacacgaaag aaacgtgtac cctcagcctg tqacggagaa tacgcctttt atcggaaaca tcgaatttca aggqatacgt ggcaactcqa aqctggaatc gatttttatt gttggqctqc ctgaatctcc tqttqttaac atcaccttta aagattttag tgctaagtct gacttgccqa tatttttgaa tcaaacgaaa cgtattgtca ttaacggtaa agaacaatag <210> 128 <211> 1209 <212> DNA <213> Callosobruchus maculatus <400> 128 atgttcgttg gaagcatcac ttggttggcc ttttgcctca tcctagtggc atgtqatact acagtatatg acgtgacgca gtttqqtgtc gattctagtq gtgcgcgtgt caqtacaagc ggtatccaqq cagtcattac aqaaqcgtcg aagaacaatq gcggtattgt ctactttcca aagggaactt atcgtaccgg accgattgag ctgaaatctg gtatcacgtt gaaggttgac gatgaggcac tcattatctt cgtagactat caagagctgt atcctccatt tgatgctgtg cttcctagcg gaaggaagat ccattttgac tacactccct tgatacgagc cttaggtcag aacaacatct ccatagtagg aagtgggatc atatatggtg taggtgaaat ttggtggaga tggttacagc aaacggagaa aagqqcaata tttgtctaca tagatgagtg tgatqgcgtc ctgataqaaq qtgtccagct taaccagagc ccacaqtatc agattaactt qaaqagtacc gagcatacag ttattaaaga ctctatattg agctccgtcg gagatgacgt cttcccggta ggccaaagga ccgacggcat caactgtgag tcatgcaggt atctgcacgt caaaaacgtc accattcgct caggcgacga tggcatcgca ctccaggcag ataagcaatt gaaggccacc gagcatgttc ttatagaaaa cagcaaaatc cttagcggcc cttcaggagt tgctattgga agcaacactq ctggcggtgt gagaaatgtc actgttcgaa attgcgttgt taatqgcaca gacagaqqtc tqtacatcaa qtcatctcga ggacaaqqcq gaacagttga aqacatccac ttctataacq tqactcttga gaaqatcggc aaagaagcaa tcgtaatcgc ttcactcttc gatggtactg atgccggttt acacgaaaga aacttagaac cacagcctgt cacagataca acgccatttg ttcggaacgt caatttccag ggaatcagag ggaatagtgc tctggaacct gtgttcatag tgggtcttcc agaggcccgc attgctaacg tcactatcaa tgacttgtcg gtgaagtctg atcgtaatgt tttttgtaat caaactgaac atattgtaat taacggtaaa gagcaatag <210> 129 <211> 1215 <212> DNA <213> Callosobruchus maculatus <400> 129 atgattgcgt tcatcattta ttttgctgcc atattgtcag caatatcggc tagccatatt tacgatgtga acaaattcgg tgcagattca acgggaagaa aacccagcac acacgccatc cagaaagcca tcaacaaggc tgcaagtaac aacggaggta ccgtccactt tccacatgga cagtacttga gtggagccat cgagttgaag tcaaatatca tcctcgattt cgcagatgac gtcgtactca aattcctgga tgatcccaaa gaatatcctc cactgcataa gaaactacct aacggtaaga ttgttaacct acaattcact ccactgatca gagcttttga ccacatcaac atcacaatca gaggcaatgc tgtactagac ggtcatgggg aaacttggtg gaaaagaatg cctcctccaa gcacaagacc agtgtttctg caaatatttt gggttcgata cctgacgctt gaaaacatca ctgtgaaaag tagtcctatg ttcaacgtca atctgaagaa ctgcgacgac gtcacgataa ggggaatcaa gataaggaac ccagctaact acgtagatcc aggacccaac actgatggta taaacgtcaa ctcttgcagg cgagtccata taacgggcgt caacataagc actggggatq attgtattgc actqqatgct aacggtaaaq gcaagaccaa aacccccaca gaagatgtgc tgatagaaga cagccacatg tatgctggac atggaggagt cagcataggt agcgtcacat cagggggatt gaggaacgta accgtcagga attgcatatt caatggtacc aacagaggtt tgttcatcaa gtcgagaaga ggcagaggtg gtttggttga agacattcac tatcacaata tccaaatggt ggacctgagg aaggaaggaa tagccattgc ggccatctat aatggaagtg acccgggact tcgtaqtaga gatttctact gggagcctgt taacqaaact accccattca tcaggaacgt tgaqttcaaa aacatccacq gcgactctgt cctcaatccc attttcatcg ttggtttgcc tgaatcacct atcgagaatg tcaccttcac taatgttaag atcgaatcta agcttgacgt atttacgaat aatacaaaaa atgttgtgat taatggcaaa gtgcttgatt cttga <210> 130 <211> 1212 <212> DNA <213> Callosobruchus maculatus <400> 130 atgtttcqat tqagcttgac qatactcata accatttcaq ctgctaacgc aqctatttac aatgtcactc tgtttggggc tgacccttca ggaactcgtc caagcaccga tgcaataaat ctagccataa gcacagctgc agctaataat ggtggcattg tccatttatc tgctggaagc tattccactg gacctattga gttgatggat ggagttgttc ttgatatcgc gcctggttgc gttgtcacat ttctcccaga tccccaatta tatcctccaa taactgtaaa gttacctgat ggaaagttga qqcaccttgc atttacacca ctaattcgtq ccacgggaaa gaqqaacgtc ggtataaaaq qaggaggcat cctcqagggg aatgqtccaa tttggtggga caqacttcca ccaccagcat cqagaccgtt tttcttctac gcttttgata gccacaacat cqtcttgacc ggtataacaa tcaggaatag tccgatgttc aatgtccata tttgtgacag taccggcatt accattacgg gaatcactat cttcaatcca ccagattgga agggtaaagg tgcaaacacg gacggcatca actgcaattc ttgtagacaa ttgcacattt ctggtgctac gattcataca ggagatgatt gtatagcgtt ggatgcagga ggaaaacgca ataagcgaat tgccactaca aatgttctta tcgaaaattg tcatatgact gctggacatq ctggggtcag tatcqgtagc gtcacgacaq qtgggctcca caatattact gtaagaaata gcctcttcga qaacacgaag aggggattgt tcattaagac aaatcgtcta aggggtggct tgatcaggga catccgttac agtaacataa acatgaataa cgttaaaggg gagggaatag ctgttgccat ggtctacaat gctaagcacc aagattatca cgatagaaac atacctaggg agcctattaa agataccact ccatttatat atgagataga atacgatggc atcagtggta cttgcggcaa tgaaccggta ctcctggtcg qccttccaga atctccagtc cgcaacattc atataaataa tttcagtgtg agatctttqa aacataaagg aaactatttg tataatacac aaaatattta catcaatgat caaaaacaat aa <210> 131 <211> 1188 <212> DNA <213> Callosobruchus maculatus <400> 131 atggtgctgc tactagctac catccagctg ttagtaatat cggcagtgag tgccattcat gacgtcacca aqtatggggc cgatcqtact ggaagagtac ctagcacgga cgccatagca agggctatca acgatgcgga qacccatgga ggagqtctaq tccattttcc accaqgcaga tacctaacaq qcccaatcga gttqaaatcc aacatgataa tggacgtcgg aqatqgtact atcatcacct tcctggacga tccagcactg tatcctccat tacacggcaa actccctgac ggccaacgaa ggatcttgcc ttttactcct ctgatcagag cttggggaca gaagaacgta gggatcagag gaaatgttat attcgacgga agaggagagg tttggtggga taggcttcca ccaccagcga ccaggcccat attcatcaac ttcttcgact gccacaacgt agttttgaag ggtttcacca tcaagagcag tccaatgttc aacgtgaaca tcctttattc caacqgcatt gtcatcgatq qtattaggat ccqcaatcca gaatcctacc atggaaaagg ccccaacact gatggcatca acgtggtatc ggtgaggagg ttgcacatca ctggggttga cgtggctaca ggggatgatt gcatagcgat caacgcttgg gggcatggca aagagcggat ccccacagaa gacgttctga ttgaagacag tcacatgtcc attgggcacg ggggtgtcag cattggcagc gtcacagcag gggggttgag gaacatcact gtaaggaact gcgtttttga cggtaccacc agaggcctgc tcatcaagac caacaqggag agaggaggca ccattgaaga catccattat gaaaatatcq ttatgaacaa tatqcqgtgg gaaggtattq cgataacgga cttqtacaac gacaaagact cgggaaggaa gccgattgga gatcagacgc cttttgtacg taacatcaag tacaataatg tacgtggtag ctctaagaaa agtcccatta tgcttatcgg tctgccagaa gctcccgttg agaatataga aatcgaaaat tgtgatatca ggtcaaagcg tccgaatttt cagttgcaga atacgagaaa cgttgttatc aatggaagga ggtattga <210> 132 <211> 1185 <212> DNA <213> Callosobruchus maculatus <400> 132 atgctgctac tagctatcat ccaqctgtta gtaatatcqq cagtaagtgc catttatgac gtcaccaggt atggcgccga tcgtactgga agagtacctt gcaccgacgc catagcaagg gctatcaagg acgcggagat ccatggggga ggtttgatcc attttccatc aggcagatac ctgacaggtc ccatcgaact gaagtccaac atggtaatgg acgtcggaac tggtactatc atcaccttcc tggacgatcc tgccctgtat cctccattag acgtcacact acctgacggc caacgaagga qcgtgccttt cactcctctg atcagagctt ggggacagaa gaacqtgggg atcagaggaa ataaagttat attcqacggg agaggaqaqa tctggtggga taqqcttccc ccaccagcca ctagacctat attcgtcaac ttcttcgact gccacaacgt ggttttgaag ggcttcacca tcaagagcag tccgatgttc aatgtgaaca tccttcattc tagtggcatc gtcatcgatg gtgtgaggat acgcaatcca gaatcctacc acggaagagg ccccaacact gatggaatca acttggtatc ggtaaggaaa gtgcacataa ctggagttga tgtcgctaca ggggatgatt qcgtggtttt caacqcgtgg ggttatctca aagagagagt ccccactgaa gatgttttqa tagagaacag ctatatgtcg gttggacatq gggctataag cataqgcagc gtcacagctg gggggttgag gaacatcacg gtgaggaact gcgtgatcga cggtaccacc agaggtctgg acatcaagac caatcggcaa cgaggaggca ccattgagaa tatcaattat tacaatatta cgatgaacag agtgaggtgg gagggtattg cgcttatcga tttgttcaac gacaacgacc caagttggaa accaatcggg gaccagaccc cgttcatacg taacatccga ttcgataata ttcgtggttc ttgtqatagg aaccccatct tggtccaagg tctacctgag gcgcctatcq aaaatatcga aatctcaaac actgacatcc ggtcgccaca accqattcga ttggagaatt ctaggaatat tgttatcaat ggaaggaqqt attga <210> 133 <211> 1149 <212> DNA <213> Sitophilus oryzae <400> 133 atgaaaataa tcgtattatt actactagcc gtagtattag cttcggcgga tcaaacagca cctggaacag catctcgccc aatcttgaca gcatcagaat caaattattt cactaccgca acttatcttc aaggatggtc gccaccttca atttcaacqa gcaaagctga ttatactgtt ggtaatqqct acaatacaat acaaqccgct gtgaatqctq ctattaatac aqqaqgcacc acaagaaaat atattaaaat taatgctgga acatatcaag aagttgttta tattcccaac acaaaggtgc ctcttacgat ttatggtgga ggcagtagcc catcagacac actgataaca ttgaacatgc ccgctcaaac tactccatca gcttataaaa gtcttgttgg gtcacttttt aattcagcgg accctgctta tagtatgtat aatagctgcg catctaaaag tggcactatt ggaactagtt qctctacagt gttttqggtt aaagcaccaq ctgttcaaat cgtaaatctc tctattqaaa attcggctaa aaataccgga gatcaqcaaq ccgtcgctct acaaactaac agtgaccaaa ttcaaatcca caatgccaga ctgttgggtc atcaagacac actgtatgct gggtcaggaa gttccagcgt cgaaaggagt tactatacca atacctatat tgaaggagat atcgattttg ttttcggtgg tggatctgct atatttgaaa gttgcacttt ctatgtaaaa gctgatcgta gaagcgacac tgccgtagtt tttgcacctg ataccgatcc tcataagatg tacggatatt tcgtttataa atctacaatt acaggagata gtgcttggtc atcctcaaag aaagcatacc taggacgtgc ttqqqattct ggcgtatcct cgtcaagtgc atacqttcca ggaacttcac ctaatggtca gctaattata aaggagaqca ccattgatgg aataattaat acttctgggc cttggacaac agcaactagc ggcagaacat attctggaaa taatgccaac agtagagacc ttaataatga caactacaat agattttggg aatataacaa ttctggaaac ggtgcctaa <210> 134 <211> 1140 <212> DNA <213> Sitophilus oryzae <400> 134 atgattqtcc aqactatcac catttcaata cttttqqtqq ttgctataca qqctqaccat caaacatatc caggcaqtgc aaqtcqccca attttatctq actcagaagc aqctcagtat acagaaacga attatttagg tggatqgaqt ccagaaaata tcaatacggt gcaaqctgat tacactgttg gtagtgggca gcaattttca tctgtacaac aagcagtaaa cgaagccatt aatgctggtg gtacatcgag gaaatacatt cgtattgaac ctggtacgta caacqaaata ttgtacatac catctactaa agtacctata acgctgtatq gtactgggaa ctcaqgagat gtgcatattt actttgacca aaqtqctcaa actactqqta ctgattatgc taatacagtc aatccaaacq qagaaagata caaqaqtgga gatccagcat attccatgta cqaqaaatgt gcaggaaatg ctaatatagg aactaqttqc tcttcagttg tatggatcaa tgcaqatgat gtgcaagtta ccaaaataac atttgagaat ccatcatcag ctgctcaagc tgtggctgtt caaaccaacg gaaaaaatat ccattttgaa gatgttcaat ttctcggctt ccagqatact ctatatttac attcaggtaa agcctacttc aataatgtca tcgtaaaagg aqacqttgat tttatttttq qcgctgcgac tqcaatcttc aagaactcqc aattgattgq ccqtqgtgac aggccacgta ctagcggtct gatttttgca ccaagcacaq atcctaataa caaatatgga ttcctagtta tcaattctct aatttctgca gacagcaata tcgaacagcg ccacggattg tctttggcta gagcgtggga ttcaggagtg agctcaggta gctatgtccc tggagtctca cctaatggcc aattagttat tcgtgaatcc tctatcgatg acggaattaa tgtcgatgct ccctattcaa cctcgaccag cggaagagaa tttaatacgg acataaatac aaaccgcaat atagatgaca atgtacataa tcgtttttgg gaatacaaaa acactggaac aggaqcttaa <210> 135 <211> 1131 <212> DNA <213> Sitophilus oryzae <400> 135 atgctattga agttggtaat actagatttt atgataattg cttctagctg ttatcacaat aacttatatc caggatcagt aagtagacca gtattatcca attcagaggc ccaaaattat atcgaattgg aatatttaca aggttggtta ccagaaagta ttcatttaag cgagcctgat tattctgttg qatatggtga accttactct tcaatacaaa ctgccattaa tgttqctctt tcagttaata tttctagaag acaatatatt tatattaaaa atggtatata ccaaqaaaca gtttatattc ctgcatccag tattccttta acgatatatg gatgtttgga cgatgctgaa gccgttcaaa tcagatttaa ccagagtgct gtgacaactg gaatcgaata cgcttctaca gtaaatccaa atggaacaaa gtataaaact ggcgaccctg cttattcaat gtatgaagag tgcgctttaa aatctacctt aggtaccaga tgttctggtg ttttttggat tatgqgtgat aatgttcaaa taagtcactt aacqqtggaa aatacctcca aaaatggcca ggcaqttgcc gtccaaacca atggtgataa aqttcagttt gaccatqtta acattaaagq atttcaggat tcgttttatt taaatggaaa tgqaaqagtt tttattaaca aaagccttat tactqgagac gtggatttcg tatttggtag tgctacggca atttttctaa atactacttt tattgcaaga gatgatcgac caaggaatac cgctatcata tttgcaccca gcacaccacc taccaaaaaa tacggctttc tagtaaaaga gtgcaccatt tcgacatctg gcaatatttc tgaatcaaca ggtttacatt tagcaagagc ttgggatggt tcagattata taccaggaac gtcagctaac ggtcaattaa ttatacgaga atcaataatc tcgaaaggcc ttaatgtttc tggtccttac agtccagcta ctagtggaag gccattcatg ggaaacattc aaaaaaatcg aqatttagat gatgttaatt acaatcgttt ttgggaatat gataatgtag atctggaata a <210> 136 <211> 1173 <212> DNA <213> Sitophilus oryzae <400> 136 atgggaagta tttcaaacta ccatttaaca ttagtagctt tagtggtgac tttacatctg gccatatctt ctcaccaaaa ctacccaggc actgagacac gaccgatttt gtctqatgat gaggcatcqa aatatcagga aqaaaactat tttgqaqatt gggaaccaga aqaaataatt attccagatg aaccagacta tatcgtaaaa gctggagaat ctatacagga aagtgttaat gctgctatca aagctggaaa ttcgtccacg cgaaaatata ttaaaattga agctggagtt tatacagaaa ctgtttatat agctggcgat gtccccttaa caatctacgg tggagattct gccagtgacg tacatatcat tcaaaatatc agtgctaata cacatatatt agattacatg aacttggtta atccagaagg tgcacqttac caggagggcq atccagcttg ggatatatac cgtgaatqcq qtcacaaaaa tqqatacatt ggtacaaatt gctctgcggt atttqtagta cgtagcgatc aatttcagct gatqaqgata actgttgaqa acggtgctac cqatqctcaa gcagttgctg ttaaaattga tgcagataaa gtccatctaa tgagtagcaa ctttttaggc ggccaagaca ctcttttcgt cggagccaac aataaaacag aagaaagagt acacgtccac atgtgctaca ttgaaggcga cacagatttc atattcgggg cagcatctgc agtgttcaac atgtgcactg taaaagttgt tggcaaacga ggaaaaaata ctgctattat atttgcacct agtactccgc ctgatagatc atatgqtttt ctggttattq acagcgtgat aactqgagat gaagtgtact taggatcaaa caaqactagc cttgcaaqaq catgggatgc aqqaqtgcaa actgctgaag actatgtagc aggatcatct ccaaatggcc aattagttat cagagattcc caaatagatg ctattatcga tgttgaccaa ccttatactg cagctgctac aagtggaaga ccgttttcta cagatataaa gactgataga aatcttgacg acaacgttca taatagactg tgggaatata acaattatgg agacgatgca taa <210> 137 <211> 1164 <212> DNA <213> Sitophilus oryzae <400> 137 atgagaagga acgccaatat ttttgtagtg tttgcactaa ttgctgtgag tctgaaatgg acaagggcag atcaccaaga gtaccctggt actgaaacaa gaccagttct gtccgattct gaagcatcca ggtataccca ggaaactgtt ttcggtgact ggggtccaca gctcatagtt gttcccgatg ttccagatta tacagtaaaa gttggagagt ctatacagaa agtgqttaat caagctttcc tqgatggtgg tgtqqacaga aagtttataa aaatcgaacc tgqaacttac gaagaatttq tqtacattaa aqqcqacgtc cctctqacaa tctacggagq aqqaaactcc gctgaagacq tqatcattgt ccaaaacata agtgccaqca tccaagcaga aqqatacgtg aatttggtca atccaaatgg cgaacgttat caagaaggcg atccagcctg ggacatttac aatggatgcg caaccaaatc tggaaacttg ggaagctgcg ctacagtgct ctggatcgag aatgatgaat ttatgatgac attggttacg gtccaaaata ccgctgtcga tgctcaaggt atcgctgttc aagttgatgc tgacaagatt aatttaatgt ttaaccggta cttggctgca caaaacactc tqtatttggg cgcccatcca gagcaaagga ttcatattca tggctcctac gtcgaaggtc aaacagatat cattqttgga cagggtqctq ccgaaataca tctttcaacc gtgaaagtaa ttggcaatga aaatatcgaa caccctgtta tatttgctcc tagtactcca gctacgcaaa cttttggatt tttggtatac aatagtactc ttacaggtga tgcgagttat ctgggatcaa acaaggttta tcttggaagg gcatgggatt ctggcatcga aaaatccgaa gactacgttc caggtgtttc cccaaatggt caactaataa taaaggcttc tcaaattgat gctatcacca acgtagaaca accctatgct ccaacttcta ccagcggcag actattctca actgatctta aqgaagatag aqatctggat gacaacacqc acaacagatt ctqqqaatat aaaaattatg gagataatgc ttga <210> 138 <211> 1128 <212> DNA <213> Callosobruchus maculatus <400> 138 atgatcaaga tqaaggtgat cttagcgttt ctggtgattt ttggagtgca ttcaatagat gcattcctta ccattagaaa caactcgttt tactacggcq aagatagggt gtttctatct ggagccaaca ttgcttggat caattttgca gaggacttcq gaagcggagq ctacqccaaa gtgaggtcca qctacgaaag cgccatcgac gacatcaqca gccatggcgg caacqtgatc agagtatggc tccacgcgga tggtcgatgg tctccaaaat gggacaagga cggcttcgct acaggagaag acacccaatc gctgatagac gatctgggac taatgcttga ctacgctgct agcaagaatg tttttgtctt tatcactctg tggactttgg aggggactcc aaagcctatg atgcatcttt actaccagga agataggctg cagtcttatt tggacagagt gctcaagcct ctcgttgtag ctctgaggga taagaaggcg ctagcaggtt gggacttagt gaatgaaccc atgggcagta taagtcaaac gcaagtagat ccaaatccat gctacgacac cactcatctt aaggacagtg gagcaggatg ggcgggcaaa accattgatt ttcggctagt ccttaagttg atcaactggc acgcagacgc tataaaatca gtggtccctg aagctctact ttcaaatgct gaaaacggag aattgttaac cacaaatgta tgtgagaagt gtagggacca ctatactgac gagtgtttga taggagcagg tggccgagcc aatggaacga ttgatttcta cgccatgcac agttatacct qqgaagggcg ctttqcaccc accagccctt tcctgcacaa ctttqatttc tacaaaaqca aqaaacctat ccttatgcaa gagttctcta ccacaatcac tqaqagtcac aacgcttcct ggaactaccg ccacatctac gaaggagatt atgtaggtat catgtcctgg cagtacaacc aatggggcaa gtgggtggac accaaggagt ctatgttcga agggatgggt gccatcagga atctcacttc ccatggaaag attaatataa agttgtag <210> 139 <211> 1134 <212> DNA <213> Callosobruchus maculatus <400> 139 atgtccacqa tcaagatgaa qqtqqtctta gcgtttttqq tgatttttgq aqtqcattca atagatgcat tccttagtgt taqaaacacg tcgttttact atggaaatga taaqqtgttt ctatctggag ccaaccttgc ttggatctat tttggcagtg acttcggaag cgggggctac gccaaagtga ggtccgccta cgaaagcgct atcgacgaca tcagcagcca tggcggcaac gcgatgagag tatggcttca cgcagacggt cgatattctc caaaatggga ccaagacggc ttcgctacag gagaagacac ccagtcgctg atagaagatc tcggactaat gctcgactac gctgctagca aqaacgtttt tatcgttctc actctgtgga ctttggaggg aactccaaag cccatgatqc atctttacta ccaqqaagat aggctgcaqq cttatttgga caqqqtcctc aagcctctcg ttgcaggcct gaaagacaag aaggcgctgg cagcttggga cttggtgaac gaacccatgg gcagtttgag tcaaacgcac aaagatccga atccatgcta cgacaccact catctcaagg acactggtgc aggatgggcg aacgaaacca ttgaatatga gaaaatcctc aagttgatca actggcacgc agacgctata aaatcagtgg accctaaggc cttagtgaca agtgctgaca acggagaatt caccaccaca actgtgtgtq agaaatgtag agatcactat acggatgagt gtttgatagg agcaggtggc agagccaaag gaacgattga cttctacgcc ttgcacagtt atacctggga agggcgctat caacccacca gccctttcaa gcacaacttt gatttctaca acagcaagaa accttacctt atggaagagt tctccaccac caacagtgag agtcacagcc cctcctggaa ctaccatcac atctacgaag gaggttttgg agggatcttg tcctggcagt acaaccaatg gggcaagtgg gtggacagca aggagtccat gttcgaaggg atggcttcca tcaggaatct cacttccaac ggaaagattg atataaagtt gtag <210> 140 <211> 1128 <212> DNA <213> Callosobruchus maculatus <400> 140 atggtcaaga tgaaagcggt cttagcgttt ctggtgattt ttggggtgca ttcaatagat gcattcctta gtgttagaaa tacgtcgttt tactacggca aagataaggt gtttctatct ggagccaaca ttgcttggtt caactttgct agggacttcg gaagcggagg ctactaccaa gtgaggtccc gcttcgaaac cgccatcaac gagatcagca gcaatggcgg caacgtgatc agagtgtggg tccacacgga cggtcaatgg tctcccaaat gggaccagaa cggcttcgct acaggagaaq acacccagtc gctqatccaa gagctcqqac taatgcttga ctacqctgct agcaagaacg tttttgtcat tctcgtcctg tggaatttgg acgtaactcc acagcccatg ttgcatcttt acaccgagga tgataaactg caggcctact tggatagagt gctcaagccc cttgttgcag gtctgaagga caagaaggcg ctggcagctt gggacttagt gaacgaaccc atgggcagtt tgagtcaatg gcaacaagat ccaaatccat gctacgacac cactcatctc aaggacactg qtgcaggatg ggcaqqcaca accattaact atcagaatat cctqaagttg atcaactqqc acgcagacgc cataaaatca gtggacccta aagccttagt qacaaacggt gaatcaggag agttcaccac cacaactata tgtgagaaat gtagggatca ctatagtgat gagtgtttga taggagcagg tggcagagct aaaggaacca ttgacttcta cgccatgcac agttatacct gggaagggcg ctatcaaccc accagccctt tcaagcacaa ctttgacttc tacaagaaaa acaaaccttt cgtcgtggaa gagttctcga ccacaaacag tgagagtcac agtcctgtct qqaactacca tcacatctac gaagggggtt ttggagggat cttqtcttgg cagtataacq aqgaaggcaa gtqqqtggac agcaaqcaqt ctatgttcga aqqaatgtct tctatcagqa atctcacttc caatqqaaag attgacataa agttgtag <210> 141 <211> 1125 <212> DNA <213> Callosobruchus maculatus <400> 141 atgaaaattg gatccgctct gctattagtg gtattgtgtc tacattcgat agatgctttc ctcagagtac aagataagaa actattttac aacaatgatc aggttttttt gtcgqgtgca aacatagctt qqtttaactt tgcaaqagat ttcggtagcq gtgcttacga ctacqtgaaa ccccgtttcq aacaagccat cqatqagatt tcgaacqctq ggggcaatgt qatcagggtt tgggtacaca tcgacggtca gtggtctcct aaatgggatg ccaacggttt cgctactggc gaagacacac catccctcat caacgaactt ggacagttgc tggaccatgc tgctcagagg aatgtcttcg tcatcttcac tctgtgggat ctcaacgtta cgccaagaca aatgctgcac ctatacagtc aqcccgaccg actacagagc tacttggaca aagtactaaa acccttggta gccgcgttga aqgacaagcc tgcattggcg gcctgggaaq tcgtcaacga accattggct agcatcacqq aaacacaaag qqatataaac ccttqctttq acaccaccca tctcaaatat agcggagcag gatggtctgg agcgcatctt ttactcaaag atatcctgcg tttcatcaac tggcatgctg acgccatcaa gttcgtggat cctaaagcgc tgtgtaccat cggaggcgct ggggaatggc tgactaccaa cgtttccccc gttaccaggg accattacac cgacgcgtgc ttgatcgcgg cqggcggaag acaacttgga actctggaca tggtaatggt ccatacctac acgttccaaq qqcgtttcgt ctcaqacacg tgtccgttca agaaacgttt cctqqactac cacaccacaa aaccgatggt catcqaagaa ttttctacaq catgtaacga qtqccacgat gcagtggcaa actatcgata cctttacgac tcagggtatt cgggggccct cqcqttccag tacaacggcc ctgggcagtg tgtagatgac catcctgtca tgttcgccgg catgtctgcc atcagaaatc tcaattacaa tgggaggatt gatattcggc tgtga <210> 142 <211> 1119 <212> DNA <213> Gastrophysa viridula <400> 142 atgaaagtcg ctgtggtatt cgtgttagcc cttggattqc attccatcga cgccttcctc aaggtccaqa acaatgcttt qtactacaac aacgacaaaq tgttcctttc cqqtqccaac atagcttggt acaactatgg atgggacttc ggcagcggcg cctacagcaa cgtcaagacc aactaccaac aagccctcga cgagatctcc caagccggcg gcaactccat cagagtttgg gtgcacatcg acggccagtg gtcgccgaag ttcgactctg agggctacgc caccggatca gacacogact ccctgatcag cgacctcggc gagctcctcg actacgcgga acagaagaac gtgttcgtca tcctctgctt gtggaacctc gccgtcgcgc ccaccaagat gctccctctc tacaccgacg acgccaagct gcagagttac ttggagaagg tgctcaagcc catggccgcc ggcctcaagg acaagaaagc gctcgccgct tgggacatca tcaacgagcc catcgggtcg ttgacccagg gcctcaccga ttcgaacccc tgctacgaca ccaacaactt gatcaacagc ggggccgact ggaccaacgt ccatctcaag cccaaggacg tgctcaagtt catcaatctc cacgccgacg ccatcaaatc ggctgacccc aaggcgctgg tcactgtggg cgagtccagt gagctgacgg ccaccaccat ttgcgagaaa tgccgcgaca tgtacagcga cagctgtctg gtcggtgctg gcggtaaagc cctgggaacc atcgatttct accagttgca cagctacact tggaacggtq ctttctcgac ctccaqtccc ttcaagaacq cagcagcagc cttcaagtcc gacaaaccca tcgtcgtggg aqaattcgcg acgtgttgca gcgagctgca aqactccgcc aagaactacc agtacctcta caactccggc ttctcggggg ccctcagctg gcagtacaac gaaggtggaa actgtgctga cccgaagtcg gttatcgacc agggaatgtc tgccatcaaa gactatactt acaacggcaa tgtccatgtg accctttga <210> 143 <211> 554 <212> PRT <213> Dendroctonus ponderosae <400> 143 Met Ala Trp Trp Gly Val Val Leu Leu Leu Ile Ala Thr Ser Ile Ser 1 5 10 15 Val Ala Val Ala Thr Val Thr Leu Thr Thr Ser Gly Leu Thr Ala Thr 25 30 Val Ser Asn Gly Asp Val Thr Val Val Phe Asn Ala His Ala Arg Val 40 45 Ser Ser Val Lys Ile Asp Asp Val Asn Ile Val Ser Thr Thr Glu Asn 55 60 Ser Phe Tyr Leu Asp Trp Asn Glu Asn Gly Glu Val Ser Phe Ser Pro 70 75 80 Ser Ser Leu Thr Val Val Asn Asp Thr Asp Ser Leu Ala His Phe Tyr 90 95 Trp Leu Gln Asp Gly Ala Ser Asp Gln Phe Glu Ile Glu Leu His Tyr 105 110 Leu Met Val Glu Gly Ile Ser Gly Val Tyr Ser Trp Ala Lys Phe Ile 120 125 Asn Ser Gln Ser Ala Asn Val Ser Leu Gly Glu Ala Arg Thr Val Tyr 135 140 Arg Phe Asn Ala Asp Leu Leu Thr Gin Gly Thr Asn Gin Aia Arq Ser 150 155 160 Gly Thr Leu Tyr Leu Tyr Ser Tyr Leu Asn Thr Gin Thr Lys Val Gin 170 175 Asp Glu Thr Trp Glu Leu Ala Asp Gly Thr Tyr Tyr Thr Lys Tyr Asp 185 190 Tyr Ala Gly Tyr Leu Arg Asn Thr Thr Tyr Gin Gly Val Tyr Gly Ser 200 205 Gly Tyr Gly Ala Phe Leu lie Ser Pro Ser Arq Glu Tyr His Ala Gly 210 215 220 Gly Pro Leu Lys Gin Asp Leu Leu lie His Gin Asp Ser Leu lie Thr 225 230 235 240 Asn Tyr Phe Val Ser Ser His Phe Gly Thr Ser Gly Leu Thr Ala Pro 245 250 255 Ser Gly Trp Thr His lie Tyr Gly Pro Trp Leu Leu Tyr Phe Asn Thr 260 265 270 Gly Thr Asn Ser Ala lie Leu Ser Asp Val Ala Thr Gin Ala Glu Thr 275 280 285 Glu Lys Asp Ser Trp Pro Tyr Ser Phe Val Asn Asp Asp Asp Tyr Pro 290 295 300 Val Asp Arg Gly Thr Val Lys Gly Thr lie Thr Gly Gin Pro Leu Ala 305 310 315 320 Thr lie Met Leu Tyr Asp Thr Glu Glu Thr Ser Tyr Asp Asp Gin Gin 325 330 335 Leu Gly Tyr Val Phe Thr Thr Glu Ser Asp Ser Ser Gly Ser Tyr Thr 340 345 350 Leu Lys Asn Val Arg Pro Gly Thr Tyr Asn Val Val Ala Tyr Pro Val 355 360 365 Ala Gly Gin Gly Ser Glu Asn Glu Ala Lys Thr Thr Val Thr Val Ala 370 375 380 Ala Gly Glu Thr Val Thr Val Ser Ser Leu Asp Leu Pro Glu Pro Asp 385 390 395 400 Asp Ile Ile Trp Asn Ile Gly Glu Thr Asn Arg Arg Ser Ser Glu Phe 405 410 415 Lys Tyr Ser Ala Glu Leu Arg Asn Tyr Leu Tyr Glu Thr Leu Pro Pro 420 425 430 Glu Thr Leu Thr Phe Thr Ile Gly Thr Ser Thr Asp Ala Asp Asp Trp 435 440 445 Tyr Tyr Ala Gln Ser Gln Ala Gly Ile Trp Thr Ile Glu Tyr Asp Asp 450 455 460 Ala Lys Asp Gly Asn Thr Arg Thr Leu Arg Val Ala Leu Ala Ala Ala 465 470 475 480 Ser Gln Ser Pro His Leu Ile Val Ser Val Asn Ser His Lys Val Gly 485 490 495 Asp Val Tyr Tyr Gly Asn Asp Gln Ala Val Tyr Arg Ser Ala Met Gln 500 505 510 Ser Gly Thr Phe His Ser Asn Val Phe Thr Val Thr Asn Ala Gln Val 515 520 525 Val Asn Gly Thr Asn Thr Ile Thr Leu Gln Val Ser Lys Gly Lys Val 530 535 540 Met Tyr Asp Ala Ile Ser Leu Gln Arg Gly 545 550 <210> 144 <211> 553 <212> PRT <213> Dendroctonus ponderosae <400> 144 Met Ala Leu Arg Gly Ser Ala Phe Leu Val Phe Ala Thr Leu Ile Ser 1 5 10 15 Val Ser Leu Ala Lys Val Thr Leu Thr Thr Asn Gly Leu Ala Ala Thr 25 30 Val Ser Asn Gly Asn Val Glu Val Val Phe Lys Ala Asp Ala Thr Val 40 45 Ser Ala Val Lys Val Asn Gly Val Asn Ile Val Ser Thr Ser Gln Lys 55 60 Thr Phe Tyr Leu Asp Trp Asn Glu Asn Gly Gln Val Thr Phe Asn Pro 70 75 80 Ser Glu Leu Lys Val Gly Glu Asn Ser Asp Ser Leu Ala His Phe Tyr 90 95 Trp Ile Gln Asn Gly Ala Ser Asn Gln Phe His Ile Glu Phe His Tyr 105 110 Val Met Val Asp Gly Val Ser Gly Ile Tyr Ser Trp Ala Lys Tyr Ile 120 125 Asn Ser Gln Ser Gly Thr Val Ser Leu Gly Glu Thr Arg Thr Ile Tyr 135 140 Arg Phe Asp Ser Ser Ile Leu Thr Gln Gly Thr Asn Gln Ala Arg Ser 150 155 160 Gly Thr Leu Tyr Leu Tyr Ser Tyr Leu Asn Thr Gln Thr Lys Val Gln 170 175 Asp Glu Thr Trp Gln Leu Ala Asp Gly Thr Tyr Tyr Thr Lys Tyr Asp 185 190 Tyr Ala Gly Tyr Leu Arg Glu Thr Ser Tyr Gln Gly Val Tyr Gly Asn 200 205 Gly Tyr Gly Ala Phe Val Ile Ser Pro Ser Arg Glu Tyr His Ala Gly 210 215 220 Gly Pro Leu Lys Gln Asp Leu Leu Ile His Gln Asp Ser Leu Ile Ala 225 230 235 240 Asn Tyr Phe Val Ser Ser His Phe Gly Thr Ser Gly Leu Ser Ala Pro 245 250 255 Ser Gly Trp Thr His lie Tyr Gly Pro Trp Leu lie Tyr Phe Asn Thr 260 265 270 Gly Ser Asp Ser Ala lie Leu Ser Asp Val Ala Asn Arg Ala Gin Thr 275 280 285 Glu Lys Ser Ser Trp Pro Tyr Ser Phe Val Asn Asp Asp Ala Tyr Pro 290 295 300 Arg Thr Arg Gly Thr Val Thr Gly Lys lie Thr Gly Gin Pro Lys Ala 305 310 315 320 Ala Val Met Leu Tyr Asp Ser Thr Giu Thr Phe Asp Asp Gin Gin Leu 325 330 335 Gly Tyr Ala Phe Thr Thr Glu Ser Asp Ser Ser Gly Ser Tyr Thr Leu 340 345 350 Thr Asn Val Arg Pro Gly Thr Tyr Asn Val Val Ala Tyr Pro Leu Ala 355 360 365 Gly Gin Gly Ser Glu Asn Leu Ala Thr Ser Thr Val Thr Val Ala Ala 370 375 380 Gly Giy Thr Ala Thr Val Ser Thr Leu Asn Leu Pro Glu Pro Gly Asn 385 390 395 400 lie lie Trp Asn lie Gly Glu Thr Asp Arg Arg Ser Asp Glu Phe Lys 405 410 415 Tyr Ser Asn Glu Pro Arg Asn Tyr Leu Tyr Glu Thr Leu Pro Pro Glu 420 425 430 Thr Leu Thr Phe Val lie Gly Ser Ser Asn Ala Ala Asn Asp Trp Tyr 435 440 445 Tyr Ser Gin Ser Lys Ala Gly lie Trp Thr lie Gin Tyr Glu Asp Thr 450 455 460 Gin Asp Giy Asn Giy Arg Thr Leu Arg Val Ala Leu Ala Ala Ala Ser 465 470 475 480 Gin Ser Pro His Leu lie Vai Asn lie Asn Giy His Lys lie Giy Asp 485 490 495 lie Tyr Phe Asp Asn Asp Gin Ser Vai Tyr Arg Ser Aia Met Gin Ser 500 505 510 Giy Lys Phe His Ser Asn lie Phe Thr Aia Asn Asn Aia Gin lie Vai 5i5 520 525 Ser Giy Thr Asn Vai lie Thr Leu Gin Vai Ser Lys Giy Gin Vai Met 530 535 540 Tyr Asp Aia lie Ser Leu Gin Arg Giy 545 550 <2i0> i45 <2ii> 555 <2i2> PRT <2i3> Dendroctonus ponderosae <400> i45 Met Leu Aia Giy Gin Vai Leu Phe Leu Leu Giy Aia Vai Aia Leu Ser i 5 10 15 Giy Leu Ser Pro Vai Asn Giy Aia Vai Ser Leu Thr Vai Asp Giy Leu 25 30 Lys Aia Thr lie Lys Asn Asp Tyr Leu Giu Leu Asn Phe Asn Ser Gin 40 45 Aia Thr lie Ser Thr Vai Asn Vai Gin Giy Thr Asn Leu Aia Aia Ser 55 60 Giy Vai Lys Thr Phe Tyr Leu Asp Trp Asn Ser Asn Giy Gin Giy Vai 70 75 80 Phe Ser Pro Ser Ser lie His lie Vai Giu Gin Thr Ser Ser Arg Vai 90 95 His Phe Tyr Trp Leu Gin Giu Asn lie Asp Asn Vai Phe Ser lie Gin 105 110 Leu His Tyr Vai Met Giu Asp Asp lie Ser Giy lie Tyr Ser Tyr Aia 120 125 Lys Tyr lie Asn Asp Lys Ser Tyr Thr Val Ser Leu Gly Glu Thr Arg 135 140 Met Val Tyr Arg Phe Asn Ala Ala lie Leu Thr Gin Gly Thr Asn Gin 150 155 160 Val Arg Ser Gly Thr Thr Pro Thr Thr Val Asp Leu Asn Gin Cys Thr 170 175 Thr Val Gin Asp Ser Thr Trp Glu Tyr Pro Asn Gly Thr Tyr Tyr Ser 185 190 Lys Tyr Asp Tyr Ala Ala Tyr lie Arg Gin lie Asn Tyr Gin Gly Val 200 205 Tyr Gly Asn Gly Phe Gly Ala Phe Val Val Ser Pro Ser Arg Glu Tyr 210 215 220 His Gly Gly Gly Pro Leu Lys Gin Asp Leu Thr Val His Gin Glu Cys 225 230 235 240 Leu Val Ala Asn Tyr Phe Val Ser Gly His Phe Gly Thr Pro Glu Val 245 250 255 Thr Ala Glu Pro Gly Trp Thr His lie Tyr Gly Pro Phe Leu Leu Tyr 260 265 270 Phe Pro Thr Gly Asn Asp Gly Ser lie Val Ser Ala Val Glu Asn Gin 275 280 285 Val Ala Ala Glu Gin Ala Lys Trp Pro Tyr Ser Phe Val Asn Asp Asp 290 295 300 Glu Tyr Pro Tyr Tyr Arg Gly Gin Val Ser Gly Thr Val Ser Gly Gin 305 310 315 320 Lys Ser Ala Thr Val Val Leu Trp Asp Ser Thr Gly Glu Glu Phe Asp 325 330 335 Gin Gin Gin Leu Gly Tyr Leu Tyr Ser Ala Glu Thr Asp Ser Lys Gly 340 345 350 Tyr Tyr Ala lie Ser Asn Val Arg Pro Gly Ser Tyr Arg lie Ala Ala 355 360 365 Tyr Pro Thr Ala Gly Leu Gly Ser Asp Ser Leu Asp Glu Ser Thr Val 370 375 380 Thr Val Thr Ala Gly Gly Arg Glu His Val Ala Leu Thr Leu Thr Glu 385 390 395 400 Pro Ser Asn Ile Ile Trp Ser Leu Gly Glu Ala Asn Arg Leu Ser Ser 405 410 415 Glu Phe Lys Tyr Ser Asp Gln Pro Arg Asn Tyr Gln Trp Glu Trp Val 420 425 430 Pro Pro Thr Glu Asn Thr Phe Val Val Gly Ser Ser Asn Pro Lys Glu 435 440 445 Asp Trp Tyr Tyr Ala Gln Ser Gln Thr Gly Ser Trp Tyr Ile Lys Tyr 450 455 460 Gln Asp Ala Pro Asp Gly Asn Ser Arg Thr Leu Arg Val Ala Ile Ala 465 470 475 480 Ala Ser Ser Lys Ser His Leu Gln Val Leu Val Asn Gly His Arg Val 485 490 495 Gly Asp Asn Tyr Tyr Asp Asn Asp His Ala Ile Tyr Arg Ser Ala Met 500 505 510 Gln Ser Gly Gln Tyr Thr Ser Asn Val Phe Thr Val Thr Asn Ala Gln 515 520 525 Val Val Asp Gly Glu Asn Thr Ile Glu Phe His Ile Ser Ile Gly Gln 530 535 540 Ile Met Tyr Asp Thr Ile Ser Leu Gln Arg Gly 545 550 555 <210> 146 <211> 555 <212> PRT <213> Dendroctonus ponderosae <400> 146 Met Arg Leu lie Leu Cys Leu Leu Leu Phe Val Leu Leu Thr Phe Val 1 5 10 15 Gly Leu Ser Pro Val Asp Ala Ala Val Thr Leu Thr Val Asp Gly Leu 25 30 Ala Ala Thr lie Arg Asn Asp Tyr Val Glu Leu Asn Phe Lys Ala Asp 40 45 Ala Thr lie Ser Thr Val Asn Val Gin Gly Thr Asn Leu Ala Ala Ser 55 60 Gly Val Lys Ser Phe Tyr Leu Asp Trp Asn Asp Asn Gly Glu Gly Val 70 75 80 Phe Asn Pro Ser Ser lie His lie Vai Giu Gin Thr Ser Ser Arg Vai 90 95 His Phe Tyr Trp Leu Gin Glu Asn lie Glu Asn Val Phe Thr lie Glu 105 110 Leu His Tyr Val Met Glu Glu Asp lie Ser Gly lie Tyr Ser Tyr Ala 120 125 Lys Tyr Thr Asn Asp Lys Ser Tyr Ala Val Thr Leu Gly Glu Thr Arg 135 140 Met Val Tyr Arg Phe Gin Ala Ser lie Leu Thr Gin Gly Thr Asn Gin 150 155 160 Val Arg Ser Gly Thr Thr Pro Thr Thr Val Asp Leu Asn Gin Cys Thr 170 175 Thr Val Gin Asp Ser Thr Trp Gin Phe Pro Asn Gly Thr Tyr Tyr Ser 185 190 Lys Tyr Asp Tyr Ala Ala Tyr lie Arg Gin lie Asn Tyr Gin Gly Val 200 205 Tyr Gly Asn Gly Phe Gly Ala Phe Val Val Ser Pro Ser Arg Glu Tyr 210 215 220 His Gly Gly Gly Pro Leu Lys Gin Asp Leu Thr Val His Gin Glu Cys 225 230 235 240 Leu Val Ala Asn Tyr Phe Leu Ser Gly His Phe Gly Thr Pro Gly Leu 245 250 255 Ser Ala Ser Pro Gly Trp Thr His Ile Tyr Gly Pro Phe Leu Leu Tyr 260 265 270 Phe Pro Thr Gly Asp Asp Gly Ser Ile Ile Ser Ala Val Gln Asn Gln 275 280 285 Val Ala Ser Glu Gln Ser Lys Trp Pro Tyr Ser Phe Val Asn Asp Ala 290 295 300 Glu Tyr Pro Thr Ser Arg Gly Lys Val Ser Gly Asn Val Ser Gly Gln 305 310 315 320 Lys Ser Ala Thr Val Val Leu Trp Asp Ser Thr Gly Glu Glu Phe Ala 325 330 335 Leu Gln Glu Leu Gly Tyr Leu Tyr Ser Thr Gln Thr Asp Ser Thr Gly 340 345 350 Tyr Tyr Ala Phe Asp Lys Val Arg Pro Gly Asn Tyr Arg Ile Ala Ala 355 360 365 Tyr Pro Thr Ala Gly Leu Gly Ser Asp Ser Leu Asp Glu Ser Thr Val 370 375 380 Thr Val Glu Ala Gly Ala Thr Gln His Val Gly Phe Thr Leu Ala Glu 385 390 395 400 Pro Asp Asn Ile Leu Trp Ser Leu Gly Glu Ala Asn Arg Leu Ser Ser 405 410 415 Glu Phe Lys Tyr Ser Asp Gln Pro Arg Asn Tyr Gln Trp Glu Trp Val 420 425 430 Pro Pro Ala Glu Asn Thr Phe Thr Ile Gly Ser Ser Asp Pro Arq Glu 435 440 445 Asp Trp Tyr Tyr Ala Gln Ser Gln Thr Gly Ser Trp Tyr Ile Lys Tyr 450 455 460 Gin Asp Thr Ala Asp Gly Ser Ala Arg Thr Leu Arg Val Ala lie Ala 465 470 475 480 Ala Ser Ser Lys Ser His Leu Gin Val lie Val Asn Gly His Arg Val 485 490 495 Gly Asp Thr Tyr Phe Asp Asn Asp Gin Ser lie Tyr Arg Ser Ala Met 500 505 510 Gin Ser Gly Arg Tyr Thr Ser Asn Val Tyr Thr Val Ser Ser Ala Gin 515 520 525 Val Val Ser Gly Glu Asn Thr lie Glu Phe His lie Ser lie Gly Gin 530 535 540 Vai Met Tyr Asp Thr lie Ser Met Gin Arg Ser 545 550 555 <210> 147 <211> 564 <212> PRT <213> Dendroctonus ponderosae <400> 147 Met Gly Pro Phe Ser lie Leu Phe Leu Ala Leu Leu Ser Thr Thr Thr 1 5 10 15 Asn Gly Phe Val Val Glu Ala Glu Asn Asp Gly Ala Phe Val Glu lie 25 30 Asn Glu Phe Asp Glu Val Thr Leu Gly Asn Asp Leu Phe Thr Val Thr 40 45 Phe Asn Ser Asn Ala Thr Ala Lys Ser Leu His Lys Asn Gly Val Asn 55 60 lie Leu Glu Asn lie Ala Glu Gly Met Gin Thr Trp Tyr Leu Asp Trp 70 75 80 Asn Gly Glu Arg Ala Tyr Phe Thr Pro Ser Leu Val Glu lie Val Arg 90 95 Asn Thr Pro Glu Gin Ala His Val Arg Phe Val Gin Glu Ala Asn Tyr 105 110 Glu Arg Glu Leu Met Tyr lie Glu Phe His Met Val Val Phe Pro Ala 120 125 Leu Ser Gly lie Tyr Gin Tyr lie Lys Leu Ser Asn Pro Thr Asp Leu 135 140 Thr Val Asp Phe Ala Glu Phe Arg Thr Val His Arg Phe Asp His Ala 150 155 160 Leu Met Pro Lys Val Ser Asn lie lie Arg Ser Glu Ala Thr Pro Asp 170 175 Val Ser Gly Arg Pro Leu lie Gin Asp Thr Thr Trp Gin Leu Ala Asp 185 190 Gly Thr Tyr Trp Ser Lys Tyr Asp Leu Cys Gly Tyr lie Arg Glu Thr 200 205 Pro Trp Phe Gly Val Tyr Gly Gly Gly Phe Gly Gly Trp lie Val Ser 210 215 220 Ala Ser Arg Glu Tyr His Ser Ala Gly Pro Leu Lys Gin Glu Leu Leu 225 230 235 240 Val His Gin Asp Ser Leu Met Leu Asn Tyr Phe His Ser Thr His Phe 245 250 255 Gly Thr Pro Asn Leu Leu Val Pro Pro Gly Trp Ser Lys Phe Phe Gly 260 265 270 Pro Tyr Leu Val Tyr lie Asn Thr Gly Ser Glu Glu Glu Val Leu Ala 275 280 285 Asp Ala Ala Asn Gin Ala Leu lie Glu Gin Ser Gin Trp Pro Tyr Ser 290 295 300 Trp Val Glu Asp Glu Glu Tyr Pro Leu Ser Arg Gly Ser Val Ser Gly 305 310 315 320 Arg Val Thr Gly Gin Thr Lys Ala Met Val Val Val Tyr Asp Ala Val 325 330 335 Glu Gin Gin Phe Asp Leu Gin Asn Leu Gly Tyr Leu Phe His Ala Glu 340 345 350 Thr Asn Glu Asp Gly Thr Phe Ala Ile Glu Asn Ile Arg Pro Gly Ser 355 360 365 Tyr Asp Val Val Ala Tyr Pro Leu Ala Gly His Gly Ser Glu Asn Leu 370 375 380 Ala Arg Lys Ser Ile Thr Val Glu Ala Gly Gly Thr His Asn Ile Gly 385 390 395 400 Asp Leu Asp Leu Pro Glu Pro Ala Asn Ile Ile Trp Ala Ile Gly Glu 405 410 415 Thr Ser Arq Arg Ser Asp Ser Tyr His Trp Ser Asp Glu Leu Arq Asn 420 425 430 Tyr Tyr Trp His Leu Leu Pro Pro Ala Asn Leu Thr Phe Glu Ile Gly 435 440 445 Lys Ser Asp Ile Gly Asn Asp Trp Tyr Tyr Ala Gln Thr Lys Thr Pro 450 455 460 Gly Ile Trp Lys Val Thr Tyr Glu Asp Glu Arg Asn Gly Tyr Gly Arg 465 470 475 480 Ile Leu Arq Val Ala Phe Ala Ala Ala Ser Arq Ser Ala Ser Val Thr 485 490 495 Val Ala Ile Asn Gly Asn Asp Ile Gly His Phe Ala Tyr Asp Asn Asp 500 505 510 Ala Thr Ile Tyr Arg Asp Ala Leu Gln Asn Gly Asn Phe His Ser Glu 515 520 525 Val Ile Ala Ile Pro Ala Asn Tyr Leu Ala Asp Gly Ser Asn Thr Ile 530 535 540 Ser Phe Glu Leu Leu Arg Gly Leu Ile Met Tyr Asp Ala Ile Ser Leu 545 550 555 560 Ala Val Asp Glu <210> 148 <211> 1778 <212> DNA <213> Dendroctonus ponderosae <400> 148 cttaggtacc atttaacgcg atggcatggt ggggcgtggt tctcttgcta attgctacct ccatatcagt tgctgtggcc acagtgacgc tcacaaccag tggactcaca gccactgtgt ccaatggcga tqtaacggtt gtcttcaacg cacatgctag agtgtcttct gtgaagatcg acgatgttaa cattgtctcc acgacqgaga atagcttcta tttggactgg aatqaaaatg gagaagttaq tttcagtcca aqttcqttaa cggttqtaaa tgacaccgac tcqctggcgc acttctactg gctccaggat ggcgcttctg atcagtttga aattgagttg cattacctga tggtggaggg aatcagtgga gtttactcgt gggccaaatt catcaacagc caaagcgcca atgtctctct tggggaggcc cggacggtct atcgcttcaa tgcggatctg ttaacccaag ggaccaacca gqcccgttcc ggaacgctct acttgtactc ctacctgaac actcagacca aagtgcaaga tqagacttgg gagctqgcgg atggaactta ctacaccaag tatqactatg ctgggtatct ccggaacact acttaccaag gcgtttatqq aagcggttac qqaqcttttc tgatctcgcc tagtcgcgaa tatcatgccg gaggcccgct caagcaggac ttgctgatcc accaggactc tttgatcacc aactactttg tgagcagtca ttttggcacc tccggattga cggccccatc tggatggact cacatctatg ggccttggct gctttatttc aacactggca ccaacagcgc catcctcagc gatgtagcaa ctcaagcaga gacggagaaa gacaqttggc cttacagttt tqtgaatgat gacqactatc cagtggacaq aggcacagtt aaaqqaacca tcactgqcca qcctctggct acqattatgc tgtacqatac ggaagaaaca tcttacgatg atcagcaact qqgttacgta ttcactacgg agtcggactc tagcgggtca tacacgctga aaaacgtccg tcctggcacc tacaacgtgg tcgcctatcc agtggctgga caaggcagtg aaaatgaagc caaaacgacc gttactgtag cagcaggtga aaccgtgaca gtcagtagcc tggacctacc ggaacccgac gacatcatct ggaacattgg cgaaaccaac cgaaggtcca gcgaattcaa gtacagtgcg gagctgcgga attatcttta cgaaactttg cccccagaga cgcttacctt caccattgga accagcactg atgctgacga ttggtattac gctcaatccc aagcaggaat ctggacaatc gaqtacgatg atgctaaaqa tggaaacacc aqqactcttc gggtcgcttt ggctgccgcc agccaaagtc ctcacttgat tgttagtgtt aacagccaca aggttgggga tgtttactac ggtaacgatc aagcggtcta cagaagcgcc atgcagagcg gcacgttcca ttcgaatgtc ttcactgtca ccaacgctca ggttgttaac ggcaccaata cgatcacctt gcaagtgagc aaaggaaaag tgatgtacga cgccattagc ttgcaaagag gctaattttt qqcaatacca agaaaaacga aatctgttac tgtccaatta tattttattt acacacaaac ttttgcaaaa ttataaatta tattacac <210> 149 <211> 1745 <212> DNA <213> Dendroctonus ponderosae <400> 149 aagagcggag cgtccaaagc actctggttt ggtaccatct gaagagatgg cattacgcgg ttcagctttt ttggtgtttg ccaccttaat ttccgtttct ttggccaaag tgaccctgac tactaatggt ctggctgcca ctgtqtccaa tggaaatgtc gaagtcgtct ttaaagctga tgccaccqtq tctgccgtta aaqtaaacgg cgttaacatc gtctctacct cqcaqaagac gttctacttq qattggaacg aaaatqgaca agtaaccttc aatccgagtg aactqaaagt aggcgaaaac tccgactcac tggctcactt ttactggatc caaaacggcg cttccaatca attccacatc gagttccatt atgttatggt ggatggcgtg agcggaatct actcatgggc caaatacatc aacagccagt ccggaacggt ctcccttgga gaaacccgaa cgatctaccg gttcgactca agcattttga cccaaggaac caaccaggct cggtctggca cgctctacct gtactcgtac ctgaacactc agactaaggt gcaagacgaq acttggcaac tggctgatgg aacttactat accaagtacg actacqctgg ttacctcaqa gagacctcct accaaggtgt ttacggaaac ggttacggag cattcgtaat ctcaccaagt cgagagtacc atgcaggagg cccgctgaag caggacttgc tgatccatca ggactctctg atcgccaact actttgtcag cagtcatttc ggcacatctg gactgagtgc tccatctgga tggactcaca tctacgggcc gtggctgatc tacttcaaca ctggaagcga cagcgctatt cttagcgacg tcgctaacag ggctcagacg qaaaagtcga gctgqcctta cagcttcgtt aacgatgatg cctacccaag aaccagaqqa accgtaaccg gtaaaatcac tgggcaqcca aaagctgccg ttatqctgta tgattctact gaaactttcg acgatcaaca attgggttac gccttcacta ctgagtccga ctcaagcgga tcatacactt taaccaacgt tcgtcctggt acttacaatg tggtcgccta cccattggct ggacagggaa gtgaaaactt ggccacatct actgtaactg tagcagcagg tggaactgca acagtcagca cccttaatct gcccgagccc ggaaatatta tctggaacat cggggagact qaccgcaggt cagacqaatt taaatacagt aacgaacccc gtaattatct gtacgaaact ctgcctccag aqactctgac ttttgtcatt ggatcaagca atqcagctaa cgactggtac tactctcaat ccaaaqctgg aatctggacc atccaatacg aaqacacgca agatggaaac ggcagaactc ttcgggtggc tttggctgcc gccagtcaaa gtcctcactt gatcgtcaac atcaacggcc acaaaatcgg tgatatttac ttcgacaacg accaatcggt ctacagaagc gccatgcaga gcggcaagtt ccattccaac atcttcaccg caaataatgc ccaaatcgtc agtggaacca atgttatcac cttgcaagtg agcaaaggac aagttatgta cgatgccatc aqcttgcaaa gaggttgatt cagtgataaa ggaataaagc attttttgtt gtttt <210> 150 <211> 1713 <212> DNA <213> Dendroctonus ponderosae <400> 150 actagtcccc ctcaacatgt tggcgggtca agttttgttc cttttaggag cggttgctct gagcggtctg aqcccagtaa atggagcggt cagcctaact gtcgacggcc ttaaagccac catcaagaac qactatttgg agctqaattt caacagtcaq gccaccatct cgactgtgaa tgttcaqqqq actaacttgg caqcaagtgg agtcaaaacc ttctacttgq actqqaacag taatggccaa ggcgtgttca gcccatccag catccacata gtggagcaga ccagcagcag ggtgcacttc tattggctgc aggaaaacat cgacaatgtg ttctcaatcc agctccacta tgtcatggag gacgatatta gtggaattta cagttacgcc aaatacatca acgacaaaag ctacacggtc tccttgggag agactcgaat ggtctacaga ttcaatgccg ccatcctcac ccaaggcacc aaccaggtgc gctctqgtac tacccccact actgtagact tgaaccaatg cactaccqtc caggactcca cctqqqaata tcccaacqqc acttattaca qcaaqtacga ctacgcagcq tacatcaggc agattaacta ccaggggqtt tatggtaatg qqtttggagc cttcgtggtc tcacccagca gggaatacca tggtggtgga ccattgaagc aagacttgac tgtccatcag gaatgtttgg tggccaacta cttcgtgagt ggtcactttg gaacgcccga agtcactgct gagccgggct ggactcacat ctatgggcct ttcttgctct acttccctac tggaaacgat ggaagcatcg tttccgctgt ggaaaatcaa gtggcggctg aacaagccaa atggccttac aqctttgtga atgacqacga atatccatat tacagaggtc aagtqagcgg aactgtttcc qqgcagaaga gcqccacagt ggttctqtqq gactcaactg qtqaqgaatt cgaccagcag caactggggt atctgtactc ggccgaaacg gactccaaag gctactatgc gatcagcaat gtgcgtcctg gcagctacag aatcgccgcc tatccgacag ccggcttggg aagcgatagt ctggatgaat caacagttac agtaactgca ggcggccgag agcacgtagc tcttaccttg acggagccca gcaacatcat ttggtctttg ggcgaagcca accgactttc cagcgagttt aagtactcgg accaaccgcg caactaccaa tgggaatggg tgccqcccac tgagaacact tttgttgttg gctcaagcaa ccccaaaqaq gactggtact acqctcaatc tcagactgga tcgtggtaca tcaagtatca agacgcgcct gatggaaact ccaggacgtt gcgagttgcc atagcagcat ccagcaagtc ccatctgcag gtcttagtga atggacaccg agtgggagac aactactacg ataacgacca tgcaatctac agaagtgcca tgcaaagtgg ccagtacact tcaaatgttt ttaccgtcac caatgctcag gtagtcgacg gggaaaacac cattgagttc catatcagta ttggacagat tatgtacgat acgatcagct tgcaaagggg ttgattgatq qaaataaatg ttttttgatt gtt <210> 151 <211> 1719 <212> DNA <213> Dendroctonus ponderosae <400> 151 acaatagtgg ccaagatgcg tctgatattg tgtctacttc tgtttgtgtt gctgactttt gtcgggttaa gcccagtaga tgcggccgtc accttaacag tggatggtct agcggctacc atcagaaacg actatgttga gctgaacttc aaagcagatg cgaccatttc tacagtgaat gttcagggca ccaacttggc agccaqtgga gttaagagct tttatctgga ttgqaacgat aatggagaaq qagtattcaa cccatccagc atccacatcq tcgaacagac caqcagtcgg gtgcatttct attggctgca agaaaacatt gagaacgttt tcaccatcga gcttcactac gtgatggagg aggacatcag cggcatctac tcctacgcca agtacaccaa cgacaaaagc tacgcagtca ccttggggga gaccagaatg gtgtatcgct tccaagcttc catcctgacc caaggcacca accaggtgcg ctctggcact acccccacta ctgtagactt gaaccaatgc actaccgtcc aqgactccac ctggcaattc cccaacggca cttattacag caaatacgac tacgcagcqt acatcaggca gatcaactac caggggqttt acggcaatgg qtttqgagcc ttcgtggtct cacccagcag ggaataccat ggtggtggac cattgaagca agacttgact gtccaccaag agtgtctggt ggccaactat ttcctcagcg gccacttcgg gactcccggc ctgagcgcca gtccgggctg gactcacatc tatggcccct tcctgcttta cttcccgact ggagacgacg gaagcatcat ttcagctgtg caaaaccagg tggcatctga gcaaagcaaa tggccctatt cctttgtgaa cgacqccgaa tatcccacct ccaggggcaa agtcagtgga aacgtttccq qqcagaagag cqcaacagtg gtgctqtqqq actcaacagg cqaaqagttt gctttgcaqq aactggggta cttqtattcc acccaaactq actccactgg ctactatgcg tttgacaaag tgcgtcctgg caactacaga attgccgctt accctacagc cggattgggg agcgatagtc tggatgaatc aacagttact gtagaggcgg gcgccacgca acatgtgggc ttcactttgg ctgaacctga taacattttg tggtctttgg gcgaagccaa ccggctttcc agcgagttta agtactcaga tcaaccgcgc aactaccaat gggagtgggt gccgcccgct gagaacactt tcacaatcgg atcaaqcgac ccgagagaaq actggtacta cgcqcaatct cagactggat cqtggtacat caaqtaccaa gacaccqctq acggttccgc aaqaacctta cgagtggcca tagcagcatc cagtaaatcc catcttcaag tcatcgtgaa tggacaccgt gtaggagaca cttacttcga taatgatcag tccatctaca ggagtgccat gcagagtggc cgctacactt ccaatgttta taccgtcagt tctgcccaag tggttagcgg cgagaatacc attgagttcc acatcagtat tggacaggtc atgtacgaca ccatcagcat gcaacgcagt tagattgttt ttgctttgtt aaatacaaat ttgtcctgg <210> 152 <211> 1755 <212> DNA <213> Dendroctonus ponderosae <400> 152 cagtccctca ctttcttttg cacctgcaga ccagaatatc gaaaatgggt ccattttcga tcctatttct cgctctgtta tccacaacga ccaatgggtt cgttgttgaa gcggaaaacg atggggcttt tqtcgaaatc aacgagtttg atgaagtcac tttgggaaac gatcttttta ccgtaacttt caactctaat gccaccgcca agtcgttgca taaaaacgga gtqaatattc tggagaatat tqccgagggc atqcaaactt ggtatctqqa ctggaatgga qaaaqggcct atttcacacc atcattagtg gaaattgtta gaaatacacc cgaacaggcg cacqtccggt tcgttcaaga agcaaattat gagagggaac tgatgtacat cgaatttcat atggtggtgt tccctgctct aagtggcatc taccagtaca tcaaactgtc caaccccact gatCtaacgg tggattttgc cgaattccga acagttcacc gcttcgacca tgccctcatg ccaaaagtta gcaatataat tcgaagcgaa gccactcctg atgtgagcgg caggccccta atccaagaca ccacttggca actagctgat ggcacqtatt ggtccaaata cgatttatgt ggctacatcc gagagacacc ttggtttgga gtttatggag gtggctttqq agggtggatc qtqtcggcga gtcgagagta ccactcagct gggcctttga agcaggaact gctggttcat caagactctt taatgctcaa ctatttccat agtacgcatt ttggtactcc caacctcctg gtgccgccag gctggtccaa attctttggg ccttacctag tttacatcaa tactgggtct gaagaggaag ttctagcgga tgcggccaat caggctctaa tcgagcaatc gcaatggcct tacagctggg tggaggatga qqagtacccc ttgtcccgcg gatctgtgtc aggaagagta accqqccaaa caaaagccat qqtagtcgtt tacqacgcgg ttgagcaqca attcgacctg caqaatctag gctatttgtt ccacgcggaa actaacgaag atggcacttt cgccattgaa aatatccgtc cgggctccta tgatgtcgta gcctatccat tggctgggca tggaagcgaa aacttagcga gaaagagcat cacggttgaa gctggaggca ctcataatat tggagatcta gatctaccag aaccagcaaa cataatctgg gctataggcg aaaccagccg gcgctctgat agttaccatt ggagcgacga qttgcgtaac tattactggc atctgcttcc tccagccaac ttaacattcg aaatcggaaa qagtgatatt gqcaatgatt ggtactatqc gcaaaccaag actcccggaa tctggaaaqt qacttatgag gatqaqagaa acggctacqq tcgcatcctg aqqqtggcgt ttgcagctqc cagcagatca gcttcagtta ctgtggccat taatggaaac qacatcggcc actttgcgta tgacaacgac gcaactattt acagagatgc gctccagaac ggcaacttcc attccgaagt tatcgctatc ccggccaatt atctcgccga cgggtcaaat acgatttctt ttgaactttt acgcggattg attatgtacg acgctatttc cttggctgta gatgaataaa gctagatata aqtgg <210> 153 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC FEATURE <222> (3) .. (3) <223> Ser, Thr, Asn or Asp <220> <221> MISC FEATURE <222> (4) (4) <223> Asp, Asn or Glu <220> <221> MISC FEATURE <222> (5) (5) <223> Ser Gly or Glu <220> <221> MISC FEATURE <222> (6) .. (6) <223> Ala, Ser or Glu <220> <221> MISC_FEATURE <222> (7) .. (7) <223> Ile or Val <220> <221> MISC_FEATURE <222> (8) (8) <223> Leu, Ile or Val <220> <221> MISC FEATURE <222> (9) (9) <223> Ser or Ala <220> <221> MISC FEATURE <222> (10) (10) <223> Asp or Ala <220> <221> MISC_FEATURE <222> (11) .. (11) <223> Val or Ala <220> <221> MISC_FEATURE <222> (12) (12) <223> Ala, Glu or Gln <220> <221> MISC_FEATURE <222> (13) (13) <223> Asn or Thr <220> <221> MISC_FEATURE <222> (14) .. (14) <223> Gln or Arg <220> <221> MISC_FEATURE <222> (15) (15) <223> Ala or Val <220> <221> MISC_FEATURE <222> (16) (16) <223> Ala, Gln, Leu or Glu <400> 153 Thr Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 15 <210> 154 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (1) (1) <223> The, Lys, Asn or Arq <220> <221> MISC FEATURE <222> (2) (2) <223> Val or lie <220> <221> MISC FEATURE <222> (3) .. (3) <223> Thr or Ser <220> <221> MISC_FEATURE <222> (6) .. (6) <223> Pro, Lys or Thr <220> <221> MISC FEATURE <222> (7) (7) <223> Ser, Lys or Leu <220> <221> MISC_FEATURE <222> (9) (9) <223> Thr, Met or Arg <220> <221> MISC_FEATURE <222> (10) .. (10) <223> Vai or lie <220> <221> MISC_FEATURE <222> (11) (11) <223> Met or Val <220> <221> MISC_FEATURE <222> (12) (12) <223> Leu or Vai <220> <221> MISC_FEATURE <222> (13) .. (13) <223> Tyr or Trp <220> <221> MISC_FEATURE <222> (15) .. (15) <223> Ser, Thr or Aia <400> 154 Xaa Xaa Xaa Gly Gin Xaa Xaa Ala Xaa Xaa Xaa Xaa Xaa Asp Xaa 1 5 10 15 <210> 155 <211> 19 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC FEATURE <222> (1) . . (1) <223> Asn, Arg or Asp <220> <221> MISC FEATURE <222> (2) (2) <223> Val or Ile <220> <221> MISC_FEATURE <222> (3) (3) <223> Val or Ala <220> <221> MISC_FEATURE <222> (7) .. (7) <223> Thr, Leu or Val <220> <221> MISC_FEATURE <222> (10) .. (10) <223> Gln, Leu or His <220> <221> MISC_FEATURE <222> (13) (13) <223> Glu or Asp <220> <221> MISC_FEATURE <222> (14) (14) <223> Asn or Ser <220> <221> MISC_FEATURE <222> (15) .. (15) <223> Leu or Glu <220> <221> MISC_FEATURE <222> (16) (16) <223> Ala or Asp <220> <221> MISC_FEATURE <222> (17) (17) <223> Glu, Lys, Thr or Arg <220> <221> MISC FEATURE <222> (18) (18) <223> Ser, Thr or Lys <220> <221> MISC FEATURE <222> (19) (19) <223> Thr or Ser <400> 155 Xaa Xaa Xaa Ala Tyr Pro Xaa Ala Gly Xaa Gly Ser Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa <210> 156 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (1) .. (1) <223> Ala, Thr or Pro <220> <221> MISC_FEATURE <222> (3) (3) <223> Ile or Ser <220> <221> MISC_FEATURE <222> (5) (5) <223> Thr, Tyr or Lys <220> <221> MISC_FEATURE <222> (6) .. (6) <223> Ile or Val <220> <221> MISC_FEATURE <222> (7) (7) <223> Lys, Gln, Glu or Thr <220> <221> MISC_FEATURE <222> (9) (9) <223> Gin, Glu or Asp <220> <22i> MISC FEATURE <222> (ii) (ii) <223> Aia, Thr or Giu <220> <22i> misc_feature <222> (i2) .. (i2) <223> Xaa can be any naturaiiy occurring amino acid <220> <221> MISC_FEATURE <222> (13) (13) <223> Asp or Asn <220> <221> MISC_FEATURE <222> (15) (15) <223> Asn, Ser, A]a or G]y <220> <221> MISC_FEATURE <222> (16) (16) <223> Giy, Ser, Aia or Thr <220> <221> MISC_FEATURE <222> (18) .. (18) <223> Thr or lie <400> 156 Xaa Gly Xaa Trp Xaa Xaa Xaa Tyr Xaa Asp Xaa Xaa Xaa Giy Xaa Xaa 1 5 10 15 Arg Xaa Leu Arg Vai <210> 157 <211> 13 <212> PRT <213> Artificiai Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (2) (2) <223> Asn or Glu <220> <221> MISC_FEATURE <222> (3) (3) <223> Val or lie <220> <221> MISC FEATURE <222> (4) (4) <223> Phe, Tyr or lie <220> <221> MISC FEATURE <222> (5) .. (5) <223> Thr or Ala <220> <221> MISC_FEATURE <222> (6) .. (6) <223> Val, Ala or lie <220> <221> MISC_FEATURE <222> (7) (7) <223> Thr, Asn, Ser or Pro <220> <221> MISC_FEATURE <222> (8) .. (8) <223> Asn, Ser or Ala <220> <221> MISC_FEATURE <222> (9) .. (9) <223> Ala or Asn <220> <221> MISC_FEATURE <222> (10) (10) <223> Gin or Tyr <220> <221> MISC_FEATURE <222> (11) (11) <223> Val, Leu or lie <220> <221> MISC_FEATURE <222> (12) .. (12) <223> Val or Ala <220> <221> MISC_FEATURE <222> (13) (13) <223> Ser, Asp or Asn <400> 157 Ser Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 1 5 10 <210> 158 <211> 16 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC FEATURE <222> (1) .. (1) <223> Gly or Ser <220> <221> MISC FEATURE <222> (3) (3) <223> Phe, Leu, Trp <220> <221> MISC FEATURE <222> (4) (4) <223> Thr or Leu <220> <221> MISC_FEATURE <222> (5) .. (5) <223> Thr or Ala <220> <221> MISC_FEATURE <222> (7) .. (7) <223> Thr or Asn <220> <221> MISC_FEATURE <222> (8) (8) <223> Val or Ile <220> <221> MISC_FEATURE <222> (9) (9) <223> Cys or Ser <220> <221> MISC_FEATURE <222> (10) .. (10) <223> Glu or Pro <220> <221> MISC_FEATURE <222> (11) (11) <223> Lys or Val <220> <221> MISC_FEATURE <222> (12) (12) <223> Cys or Thr <220> <221> MISC FEATURE <222> (15) (15) <223> His or Met <400> 158 Xaa Glu Xaa Xaa Xaa Thr Xaa Xaa Xaa Xaa Xaa Xaa Arg Asp Xaa Tyr 1 5 10 15 <210> 159 <211> 33 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (3) (3) <223> Ser or Ala <220> <221> MISC_FEATURE <222> (5) .. (5) <223> Thr, Ala or Cys <220> <221> MISC_FEATURE <222> (6) (6) <223> Asn, Cys or Ile <220> <221> MISC_FEATURE <222> (7) (7) <223> Ser, Asn or Thr <220> <221> MISC_FEATURE <222> (9) .. (9) <223> Ser, Cys or Leu <220> <221> MISC_FEATURE <222> (10) .. (10) <223> His or Gln <220> <221> MISC_FEATURE <222> (11) (11) <223> Ser, Asp or Asn <220> <221> MISC FEATURE <222> (12) (12) <223> Ala, Pro or Ser <220> <221> MISC FEATURE <222> (13) (13) <223> Ser, Val or Ala <220> <221> MISC_FEATURE <222> (14) . . (14) <223> Trp, Ala or Lys <220> <221> MISC_FEATURE <222> (17) (17) <223> Arq, His or Gin <220> <221> MISC_FEATURE <222> (18) (18) <223> His or Tyr <220> <221> MISC_FEATURE <222> (19) .. (19) <223> lie or Leu <220> <221> MISC_FEATURE <222> (21) .. (21) <223> Glu, Asp or Asn <220> <221> MISC_FEATURE <222> (22) (22) <223> Gly or Ser <220> <221> MISC FEATURE <222> (23) (23) <223> Gly or Asp <220> <221> MISC FEATURE <222> (24) .. (24) <223> Phe or Tyr <220> <221> MISC_FEATURE <222> (25) (25) <223> Gly, Ser or Val <220> <221> MISC_FEATURE <222> (27) (27) <223> lie or Ala <220> <221> MISC_FEATURE <222> (28) (28) <223> Leu or Met <220> <221> MISC_FEATURE <222> (29) (29) <223> Ser or Ala <220> <221> MISC_FEATURE <222> (30) .. (30) <223> Trp or Phe <400> 159 Glu Phe Xaa Thr Xaa Xaa Xaa Glu Xaa Xaa Xaa Xaa Xaa Xaa Asn Tyr 1 5 10 15 Xaa Xaa Xaa Tyr Xaa Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Gln Tyr 25 30 Asn <210> 160 <211> 20 <212> FRT <213> Artifioial Sequenoe <220> <223> Consensus sequenoe <220> <221> MISC_FEATURE <222> (1) (1) <223> Leu or Glu <220> <221> MISC_FEATURE <222> (5) .. (5) <223> Leu, Val, Fhe or Thr <220> <221> MISC_FEATURE <222> (6) (6) <223> Thr, Asp, His or Asn <220> <221> MISC_FEATURE <222> (7) (7) <223> Val, Lys, Ala or Gly <220> <221> MISC FEATURE <222> (8) (8) <223> Lys, Thr, Leu or Val <220> <221> MISC FEATURE <222> (11) (11) <223> Asp, Ser or Asn <220> <221> misc_feature <222> (13) .. (14) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (15) (15) <223> Arq, Ile, Phe or Val <220> <221> misc_feature <222> (16) (16) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (17) .. (17) <223> Leu, Phe or Val <220> <221> misc_feature <222> (18) (18) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (19) (19) <223> Phe or Tyr <400> 160 Xaa Tyr Pro Pro Xaa Xaa Xaa Xaa Leu Pro Xaa Gly Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Thr <210> 161 <211> 20 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC FEATURE <222> (4) (4) <223> Cys, Val or Leu <220> <221> misc_feature <222> (5) .. (5) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (6) .. (6) <223> Ser or Pro <220> <221> MISC_FEATURE <222> (7) (7) <223> Cys or Va] <22 0> <221> MISC_FEATURE <222> (9) (9) <223> Tyr, Arg or Gln <220> <221> MISC_FEATURE <222> (10) .. (10) <223> Leu or Val <220> <221> MISC_FEATURE <222> (12) (12) <223> Ile or Val <220> <221> MISC_FEATURE <222> (13) (13) <223> Thr, Ser or Lys <220> <221> MISC_FEATURE <222> (14) .. (14) <223> Gly or Asn <220> <221> MISC_FEATURE <222> (15) .. (15) <223> Val or Ala <220> <221> MISC_FEATURE <222> (16) (16) <223> Thr, Asp or Asn <220> <221> MISC FEATURE <222> (17) (17) <223> lie or Val <220> <221> misc_feature <222> (18) (18) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (19) . . (19) <223> Thr or Ser <400> 161 Gly lie Asn Xaa Xaa Xaa Xaa Arg Xaa Xaa His Xaa Xaa Xaa Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Giy <210> 162 <211> 10 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (1) (1) <223> lie or Val <220> <221> MISC FEATURE <222> (2) (2) <223> Ala or Val <220> <221> MISC FEATURE <222> (3) .. (3) <223> Leu, Iie,Phe or Met <220> <221> MISC_FEATURE <222> (4) .. (4) <223> Asp, Asn, Gin or Lys <220> <221> MISC_FEATURE <222> (6) (6) <223> Asp, Trp, Asn, Gly <220> <221> MISC FEATURE <222> (7) (7) <223> Gly, Leu, Met or Lys <220> <221> misc_feature <222> (8) .. (8) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (9) .. (9) <223> Gly, Leu, Asp or Arg <220> <221> MISC_FEATURE <222> (10) (10) <223> Lys, Arg or Asn <400> 162 Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa Xaa Xaa 1 5 10 <210> 163 <211> 33 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (2) (2) <223> Glu, Lys, Gln or Ser <220> <221> MISC_FEATURE <222> (3) (3) <223> Lys, Ala, Gly or Asp <220> <221> MISC_FEATURE <222> (5) .. (5) <223> Pro or no amino acid <220> <221> misc_feature <222> (6) (6) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (7) (7) <223> Ala, Thr or Val <220> <221> misc_feature <222> (8) (8) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC FEATURE <222> (9) .. (9) <223> Gly or Lys <220> <221> MISC FEATURE <222> (12) (12) <223> Phe, Tyr or Leu <220> <221> MISC_FEATURE <222> (13) (13) <223> Ile or Met <220> <221> MISC_FEATURE <222> (15) (15) <223> Thr, Asn or Ser <220> <221> MISC_FEATURE <222> (16) .. (16) <223> Phe or Tyr <220> <221> MISC_FEATURE <222> (17) (17) <223> Gln, Glu or Gly <220> <221> MISC_FEATURE <222> (20) (20) <223> Pro or Ser <220> <221> MISC_FEATURE <222> (21) .. (21) <223> Gln, Glu or Ser <220> <221> MISC_FEATURE <222> (23) .. (23) <223> Ser or Asn <220> <221> MISC_FEATURE <222> (24) (24) <223> Thr or Ala <220> <221> MISC FEATURE <222> (26) (26) <223> Arq or Lys <220> <221> MISC FEATURE <222> (27) (27) <223> Thr or Ala <220> <221> MISC_FEATURE <222> (28) . . (28) <223> Ile or Val <220> <221> MISC_FEATURE <222> (30) (30) <223> Gln or Ser <220> <221> MISC_FEATURE <222> (31) (31) <223> Thr, Pro or Gly <220> <221> MISC FEATURE <222> (32) .. (32) <223> Thr, Cys or Ile <400> 163 Cys Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Pro Ser Xaa Xaa Asn Xaa Xaa 1 5 10 15 Xaa Arg Gly Xaa Xaa Glu Xaa Xaa Trp Xaa Xaa Xaa Pro Xaa Xaa Xaa 25 30 Cys <210> 164 <211> 7 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (2) (2) <223> Val or lie <220> <221> MISC FEATURE <222> (3) (3) <223> Gly, Ser or Asp <220> <221> MISC FEATURE <222> (4) (4) <223> Pro, Thr or Ala <220> <221> MISC_FEATURE <222> (5) . . (5) <223> Tyr, Trp or His <220> <221> MISC_FEATURE <222> (5) (5) <223> Tyr, Trp or His <220> <221> MISC_FEATURE <222> (6) (6) <223> Asn, Ser, Ala or Lys <400> 164 Cys Xaa Xaa Xaa Xaa Xaa Cys 1 5 <210> 165 <211> 12 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC FEATURE <222> (1) (1) <223> Asn, Phe or Lys <220> <221> MISC FEATURE <222> (2) .. (2) <223> Ile or Leu <220> <221> MISC_FEATURE <222> (3) (3) <223> Lys or Leu <220> <221> misc_feature <222> (7) (7) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC FEATURE <222> (8) (8) <223> Phe, His, Ala or Gln <220> <221> misc_feature <222> (11) (11) <223> Xaa can be any naturally occurring amino acid <400> 165 Xaa Xaa Xaa Asn Cys Pro Xaa Xaa Cys Val Xaa Ile 1 5 10 <210> 166 <211> 32 <212> PRT <213> Artificia] Sequence <220> <223> Consensus sequence <220> <221> MISC FEATURE <222> (2) .. (2) <223> Ala or Ser <220> <221> MISC_FEATURE <222> (3) (3) <223> Ser, Asn, Ala or Gln <220> <221> misc_feature <222> (4) (4) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (5) .. (5) <223> Tyr or Phe <220> <221> MISC_FEATURE <222> (6) .. (6) <223> Thr, Ile or Gln <220> <221> MISC_FEATURE <222> (7) (7) <223> Glu, Thr or Gln <220> <221> MISC_FEATURE <222> (8) (8) <223> Glu, Ala, Thr or Leu <220> <221> MISC FEATURE <222> (9) (9) <223> Asn, Thr or Glu <220> <221> MISC FEATURE <222> (10) .. (10) <223> Tyr or Val <220> <221> MISC_FEATURE <222> (11) (11) <223> Leu or Phe <220> <221> MISC_FEATURE <222> (12) (12) <223> Gly or Gln <220> <221> MISC_FEATURE <222> (13) (13) <223> Gly or Asp <220> <221> MISC_FEATURE <222> (15) .. (15) <223> Ser, Leu, Glu or Gly <220> <221> MISC_FEATURE <222> (17) (17) <223> Glu, Pro or Gln <220> <221> MISC_FEATURE <222> (18) (18) <223> Ser, Asn, Glu or Leu <220> <221> misc_feature <222> (20) .. (20) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (21) (21) <223> Thr, Leu, Ile or Val <220> <221> MISC_FEATURE <222> (22) (22) <223> Pro, Ser or Val <220> <221> MISC FEATURE <222> (23) (23) <223> Asp, Lys, Gin or Giu <220> <221> MISC FEATURE <222> (24) (24) <223> No amino acid or Giu or Lys <220> <221> MISC_FEATURE <222> (25) .. (25) <223> Pro or Ala <220> <221> MISC_FEATURE <222> (28) (28) <223> Thr, Ser or lie <220> <221> MISC_FEATURE <222> (30) (30) <223> Gly or Lys <220> <221> misc_feature <222> (31) .. (31) <223> Xaa can be any naturally occurring amino acid <400> 166 Glu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp Xaa Pro 1 5 10 15 Xaa Xaa lie Xaa Xaa Xaa Xaa Xaa Xaa Asp Tyr Xaa Val Xaa Xaa Gly 25 30 <210> 167 <211> 21 <212> PRT <213> Artificial Sequence <220> <223> Consensus sequence <220> <221> MISC_FEATURE <222> (1) (1) <223> Thr or Ile <220> <221> MISC_FEATURE <222> (2) (2) <223> Thr, His or Gin <220> <221> MISC FEATURE <222> (3) (3) <223> Gly, Pro, lie or Ala <220> <221> misc_feature <222> (4) .. (4) <223> Xaa can be any naturally occurring amino acid <220> <221> MISC_FEATURE <222> (5) .. (5) <223> Asp, Ala, Glu or Gly <220> <221> MISC_FEATURE <222> (7) (7) <223> Ala, Lys, Met or Val <220> <221> MISC_FEATURE <222> (8) (8) <223> Asn or Ser <220> <221> MISC_FEATURE <222> (9) .. (9) <223> Leu or Thr <220> <221> MISC_FEATURE <222> (11) (11) <223> Asn or no amino acid <220> <221> MISC_FEATURE <222> (12) (12) <223> Pro or no amino acid <220> <221> MISC_FEATURE <222> (13) (13) <223> Asn or Glu or no amino acid <220> <221> MISC_FEATURE <222> (15) .. (15) <223> Glu, Ser, Thr or Ala <220> <221> MISC_FEATURE <222> (16) (16) <223> Arg, Leu or Lys <220> <221> MISC_FEATURE <222> (17) (17) <223> Tyr or Phe <220> <221> MISC_FEATURE <222> (18) (18) <223> Lys, Gin or Asn <220> <221> MISC_FEATURE <222> (19) .. (19) <223> Ser, Glu or Thr <220> <221> MISC_FEATURE <222> (20) (20) <223> Gly or Ala <400> 167 Xaa Xaa Xaa Xaa Xaa Tyr Xaa Xaa Xaa Val Xaa Xaa Xaa Gly Xaa Xaa 1 5 10 15 Xaa Xaa Xaa Xaa Asp

Claims (22)

1. Claims 1. A Coleoptera polysaccharide degrading enzyme comprising an amino sequence which is: a) at least about 55% identical to SEQ ID NO:67; b) at least about 45% identical to SEQ ID NO:143; c) at least about 40% identical to SEQ ID NO:55; d) at least about 65% identical to SEQ ID NO:1; e) at least about 86% identical to SEQ ID NO: 16; at least about 81% identical to SEQ ID NO:26; g) at least about 98% identical to SEQ ID NO:5 1 or 62; or or an enzymatically functional fragment or variant of any of these.
2. 2. An enzyme according to claim 1 which is at least about 55% identical to SEQ ID NO:67 and one or more of SEQ ID NOs:68-71 and comprises at least one of SEQ ID NOs:158 and 159.
3. 3. An enzyme according to claim 1 which is at least about 45% identical to SEQ ID NO:143 and one or more of SEQ ID NOs:144-147 and comprises at least one of SEQ IDNOs:153-157.
4. 4. An enzyme according to claim 1 which is at least about 40% identical to SEQ ID NO:55 and one or more of SEQ ID NOs:56-61 and comprises at least one of SEQ ID NOs:160-162.
5. 5. An enzyme according to claim 1 which is at least about 65% identical to SEQ ID NO:1 and one or more of SEQ ID NOs:2-15 and does not comprise a cellulose binding domain.
6. 6. An enzyme according to claim 1 which is at least about 86% identical to SEQ ID NO:16 and one or more of SEQ ID NOs: 17-24 and comprises at least one of SEQ ID NOs:163 and 164.
7. 7. An enzyme according to claim 1 which is at least about 81% identical to SEQ TD NO:26 and one or more of SEQ TD NOs:27-50 or 52-54 and comprises SEQ ID NO:165, or which is at least about 98% identical to SEQ ID NO:51 and comprises SEQ ID NO:165.
8. 8. An enzyme according to claim 1 which is at least about 98% identical to SEQ ID NO:62 and one or more of SEQ ID NOs:63-66 and comprises at least one of SEQ ID NOs:166 and 167.
9. 9. A method of degrading plant material comprising exposing the material to at least one polysaccharide degrading enzyme according to claim 1-8.
10. 10. A method according to any preceding claim comprising expressing the enzyme in a micro-organism.
11. 11. A method according to claim 10 comprising contacting the plant material with the micro-organism and/or contacting the plant material with an extract of the micro-organism and/or an extracellular growth rnediun1 of the micro-organism.
12. 12. A method according to any of claims 9-11 wherein the amino acid sequence of the enzyme is encoded by any of polynucleotide sequences SEQ ID NOs:72-142 or 148-152.
13. 13. A micro-organism comprising at least one polysaccharide degrading enzyme according to any of claims 1-8.
14. 14. A micro-organism according to claim 13 comprising at least one of the polynucleotide sequences SEQ ID NOs:72-142 or 148-152 or a micro-organism equivalent thereof.
15. 15. A micro-organism according to claim 13 or 14 which is a yeast or a bacterium.
16. 16. A plant comprising at least one polysaccharide degrading enzyme according to any of claims 1-8.
17. 17. A plant according to claim 16 comprising at least one of the polynucleotide sequences SEQ ID NOs:72-142 and 148-152 andlor a portion thereof, or a plant equivalent of such a sequence or portion.
18. 18. A polynucleotide encoding a polypeptide according to claim 1.
19. 19. A polynucleotide according to claim 18 comprising at least one of SEQ ID NOs:72- 142 and 148-152.
20. 20. An expression vector comprising at least one polynucleotide according to claim 18 or 19.
21. 21. A method of controlling an insect pest comprising feeding to the insect a plant according to claim 16 or 17.
22. 22. A method according to claim 21 wherein RINA interference occurs in the insect.