EA202290114A1 - Способ отбора рекомбинантных белков с высоким содержанием m6p - Google Patents

Способ отбора рекомбинантных белков с высоким содержанием m6p

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Publication number
EA202290114A1
EA202290114A1 EA202290114A EA202290114A EA202290114A1 EA 202290114 A1 EA202290114 A1 EA 202290114A1 EA 202290114 A EA202290114 A EA 202290114A EA 202290114 A EA202290114 A EA 202290114A EA 202290114 A1 EA202290114 A1 EA 202290114A1
Authority
EA
Eurasian Patent Office
Prior art keywords
rhgaa
column
aex
bioreactor
eluting
Prior art date
Application number
EA202290114A
Other languages
English (en)
Inventor
Хун В. До
Расселл Готшалль
Original Assignee
Амикус Терапьютикс, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Амикус Терапьютикс, Инк. filed Critical Амикус Терапьютикс, Инк.
Priority claimed from US15/473,994 external-priority patent/US10227577B2/en
Publication of EA202290114A1 publication Critical patent/EA202290114A1/ru

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)

Abstract

Описан способ получения очищенной рекомбинантной кислой альфа-глюкозидазы человека (rhGAA), где очищенная rhGAA содержит аминокислотную последовательность, которая по меньшей мере на 98% идентична SEQ ID NO: 2, при этом способ включает культивирование в биореакторе клеток-хозяев, которые секретируют rhGAA; удаление среды из биореактора; фильтрование среды с получением фильтрата; загрузку фильтрата в колонку для анионообменной хроматографии (AEX) для захвата rhGAA; элюирование первого белкового продукта из колонки для AEX; загрузку rhGAA, элюированной из колонки для AEX, в колонку для аффинной хроматографии на иммобилизованных ионах металлов (IMAC) и элюирование rhGAA из колонки для IMAC.
EA202290114A 2017-03-30 2017-03-30 Способ отбора рекомбинантных белков с высоким содержанием m6p EA202290114A1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15/473,994 US10227577B2 (en) 2016-03-30 2017-03-30 Method for selection of high M6P recombinant proteins

Publications (1)

Publication Number Publication Date
EA202290114A1 true EA202290114A1 (ru) 2022-03-29

Family

ID=67399668

Family Applications (2)

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EA201892185A EA039750B1 (ru) 2017-03-30 2017-03-30 Способ отбора рекомбинантных белков с высоким содержанием m6p
EA202290114A EA202290114A1 (ru) 2017-03-30 2017-03-30 Способ отбора рекомбинантных белков с высоким содержанием m6p

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EA201892185A EA039750B1 (ru) 2017-03-30 2017-03-30 Способ отбора рекомбинантных белков с высоким содержанием m6p

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EA (2) EA039750B1 (ru)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020073438A1 (en) * 1995-08-02 2002-06-13 Reuser Arnold J. Methods of purifying human acid alpha-glucosidase
US6458574B1 (en) * 1996-09-12 2002-10-01 Transkaryotic Therapies, Inc. Treatment of a α-galactosidase a deficiency
WO2005077093A2 (en) * 2004-02-06 2005-08-25 Biomarin Pharmaceutical Inc. Manufacture of highly phosphorylated lysosomal enzymes and uses thereof
MX2007014470A (es) * 2005-05-17 2008-02-06 Amicus Therapeutics Inc Metodo para el tratamiento de enfermedad de pompe usando derivados de 1-desoxinojirimicina.
EP2946785B1 (en) * 2008-02-12 2018-10-24 Amicus Therapeutics, Inc. Method to predict response to pharmacological chaperone treatment of diseases
JP5990102B2 (ja) * 2009-09-29 2016-09-07 ユニフェルシテイト ヘント ホスホ−６−マンノースへのマンノース−１−ホスホ−６−マンノース結合の加水分解
SG189108A1 (en) * 2010-09-29 2013-05-31 Oxyrane Uk Ltd Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated n-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
KR20190114003A (ko) * 2012-03-15 2019-10-08 옥시레인 유케이 리미티드 폼페병의 치료를 위한 방법 및 물질
RS65066B1 (sr) * 2014-09-30 2024-02-29 Amicus Therapeutics Inc Visoko potentna kisela alfa-glukozidaza sa pojačanim ugljenim hidratima

Also Published As

Publication number Publication date
EA039750B1 (ru) 2022-03-10
EA201892185A1 (ru) 2019-07-31

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