EA202193200A1 - Выделение фетальных клеток с помощью facs - Google Patents

Выделение фетальных клеток с помощью facs

Info

Publication number
EA202193200A1
EA202193200A1 EA202193200A EA202193200A EA202193200A1 EA 202193200 A1 EA202193200 A1 EA 202193200A1 EA 202193200 A EA202193200 A EA 202193200A EA 202193200 A EA202193200 A EA 202193200A EA 202193200 A1 EA202193200 A1 EA 202193200A1
Authority
EA
Eurasian Patent Office
Prior art keywords
fetal cells
facs
isolation
fetal
cells
Prior art date
Application number
EA202193200A
Other languages
English (en)
Inventor
Болетта Хестбек Николаисен
Дэвид Матиас Мартин Лейдинг Кёльвро
Инга Боск Кристенсен
Катарина Раун
Лина Даль Йеппесен
Лотта Хатт
Палле Скельда Йенсен
Рипудаман Сингх
Original Assignee
Арседи Байотек Апс
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Арседи Байотек Апс filed Critical Арседи Байотек Апс
Publication of EA202193200A1 publication Critical patent/EA202193200A1/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/36—Gynecology or obstetrics
    • G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour

Abstract

Настоящее изобретение относится к автоматизированным способам выделения фетальных клеток из образца, такого как образец крови, полученный от беременной женщины. Выделенные клетки плода можно использовать для выявления генетических аномалий в ДНК плода.
EA202193200A 2019-06-07 2020-06-08 Выделение фетальных клеток с помощью facs EA202193200A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19179087 2019-06-07
PCT/EP2020/065832 WO2020245459A1 (en) 2019-06-07 2020-06-08 Isolation of fetal cells using facs

Publications (1)

Publication Number Publication Date
EA202193200A1 true EA202193200A1 (ru) 2022-03-17

Family

ID=66793879

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202193200A EA202193200A1 (ru) 2019-06-07 2020-06-08 Выделение фетальных клеток с помощью facs

Country Status (16)

Country Link
US (2) US11573229B2 (ru)
EP (2) EP3980773B1 (ru)
JP (1) JP2022536121A (ru)
KR (1) KR20220047929A (ru)
CN (1) CN114041053A (ru)
AU (1) AU2020289708A1 (ru)
BR (1) BR112021024474A2 (ru)
CA (1) CA3142663A1 (ru)
EA (1) EA202193200A1 (ru)
ES (1) ES2955791T3 (ru)
IL (2) IL288621B1 (ru)
MX (1) MX2021015044A (ru)
PL (1) PL3980773T3 (ru)
SG (1) SG11202113242QA (ru)
WO (1) WO2020245459A1 (ru)
ZA (1) ZA202110215B (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113087793B (zh) * 2021-05-12 2022-04-12 福州迈新生物技术开发有限公司 抗ck14蛋白单克隆抗体及其细胞株、制备方法和应用
CN112980779B (zh) 2021-05-20 2021-08-24 广州凯普医药科技有限公司 一种从孕妇宫颈脱落细胞中分离胎盘滋养层细胞的方法
WO2023104773A1 (en) * 2021-12-06 2023-06-15 Arcedi Biotech Aps Isolation and diagnostics of fetal cells

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5641628A (en) 1989-11-13 1997-06-24 Children's Medical Center Corporation Non-invasive method for isolation and detection of fetal DNA
US5629147A (en) 1992-07-17 1997-05-13 Aprogenex, Inc. Enriching and identifying fetal cells in maternal blood for in situ hybridization
JPH08511205A (ja) 1993-04-02 1996-11-26 ジェオ リサーチ エス．アー．エール．エル． 低温流体の噴射による切断設備と方法
IL110364A0 (en) 1993-07-19 1994-10-21 Aprogenex Inc Enriching and identifying fetal nucleated erythrocytes in maternal blood for in situ hybridization
US5714325A (en) 1993-09-24 1998-02-03 New England Medical Center Hospitals Prenatal diagnosis by isolation of fetal granulocytes from maternal blood
US5648222A (en) 1994-07-27 1997-07-15 The Trustees Of Columbia University In The City Of New York Method for preserving cells, and uses of said method
GB9603249D0 (en) 1996-02-16 1996-04-17 Univ Nottingham Foetal cell analysis
GB0009179D0 (en) 2000-04-13 2000-05-31 Imp College Innovations Ltd Non-invasive prenatal diagnosis
AU5676701A (en) 2000-05-16 2001-11-26 Kyowa Hakko Kogyo Co. Ltd. Novel method of inducing the differentiation of embryonic stem cells into ectodermal cells and use thereof
WO2002074979A2 (en) 2001-03-20 2002-09-26 Ortho-Clinical Diagnostics, Inc. Expression profiles and methods of use
US20030036100A1 (en) 2001-04-10 2003-02-20 Imperial College Innovations Ltd. Simultaneous determination of phenotype and genotype
FR2824144B1 (fr) 2001-04-30 2004-09-17 Metagenex S A R L Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel
PT1572087E (pt) 2002-03-08 2008-07-11 Pdl Biopharma Inc Anticorpos contra o antigénio de cancro tmeff2 e suas utilizações
US20050181429A1 (en) 2003-04-03 2005-08-18 Monaliza Medical Ltd. Non-invasive prenatal genetic diagnosis using transcervical cells
US20040197832A1 (en) 2003-04-03 2004-10-07 Mor Research Applications Ltd. Non-invasive prenatal genetic diagnosis using transcervical cells
CN101137761B (zh) 2005-03-18 2010-12-08 香港中文大学 产前诊断和监测的标记
JP2008543277A (ja) 2005-05-11 2008-12-04 ジェネティック テクノロジーズ リミテッド 胎児細胞を富化する方法
ATE486288T1 (de) 2005-12-08 2010-11-15 Fcmb Aps Nachweis fötaler zellen aus mütterlichem blut
GB0616045D0 (en) 2006-08-11 2006-09-20 Univ Bristol Blood cell separation
US8338113B2 (en) 2007-05-01 2012-12-25 Tel Hashomer Medical Research Infrastructure And Services Ltd. Methods for detecting fetal cells in the maternal blood
EP2250497B1 (en) 2008-02-18 2014-09-10 Genetic Technologies Limited Cell processing and/or enrichment methods
DK2385992T3 (en) 2009-01-07 2015-04-07 Arcedi Biotech Aps Enriching and identifying fetal cells in maternal blood and ligand for such use
CN102458665A (zh) 2009-04-22 2012-05-16 临床基因组学股份有限公司 用于从生物学样品中分离靶生物实体的方法和仪器
CA2817390C (en) 2010-11-09 2021-02-09 Quantibact A/S Enrichment and identification of fetal cells in maternal blood and ligands for such use

Also Published As

Publication number Publication date
US11573229B2 (en) 2023-02-07
WO2020245459A1 (en) 2020-12-10
CA3142663A1 (en) 2020-12-10
EP3980773B1 (en) 2023-07-12
CN114041053A (zh) 2022-02-11
IL288621A (en) 2022-02-01
US20230130312A1 (en) 2023-04-27
ES2955791T3 (es) 2023-12-07
AU2020289708A1 (en) 2022-01-20
EP4242652A2 (en) 2023-09-13
IL308999A (en) 2024-01-01
EP3980773A1 (en) 2022-04-13
PL3980773T3 (pl) 2023-11-27
MX2021015044A (es) 2022-04-06
US20210199664A1 (en) 2021-07-01
JP2022536121A (ja) 2022-08-12
EP4242652A3 (en) 2023-12-06
SG11202113242QA (en) 2021-12-30
KR20220047929A (ko) 2022-04-19
EP3980773C0 (en) 2023-07-12
BR112021024474A2 (pt) 2022-01-18
IL288621B1 (en) 2024-01-01
ZA202110215B (en) 2023-06-28

Similar Documents

Publication Publication Date Title
EA202193200A1 (ru) Выделение фетальных клеток с помощью facs
MX2017005159A (es) Composiciones y metodos para detectar un virus de rna.
EP2569453A4 (en) Nucleic Acid ISOLATION METHOD
BR112015032031A8 (pt) método para estimar uma fração de ácido nucleico em uma amostra de teste de uma mulher grávida
EA201792389A1 (ru) Пцр в реальном времени для мультиплексной оптимизированной амплификации с несоответствиями (moma) и для оценки неклеточной днк
CY1124376T1 (el) Συνδυαστικη αναλυση μεγεθους και καταμετρησης στο μητρικο πλασμα για την ανιχνευση εμβρυϊκων υποχρωμοσωμικων ανωμαλιων
MX2022009242A (es) Producto en desarrollo de analisis de secuenciacion de alineacion y variante.
GB2526736A (en) Methods, compositions, and kits for nucleic acid analysis
WO2012149339A3 (en) Quantification of a minority nucleic acid species
EA201892490A1 (ru) Пцр с мультиплексной оптимизированной амплификацией неправильно спаренных оснований (moma) в реальном времени для анализа состояния плода
GB2525104A (en) Massively parallel single cell analysis
EP3722438A3 (en) Method for production of moth pheromones in yeast
WO2012142611A3 (en) Sequencing small amounts of complex nucleic acids
IN2012DN03139A (ru)
GB201205769D0 (en) Methods for preparing samples for nucleic acid amplification
EA202091219A1 (ru) Культивирование плаценты с целью выделения экзосом
MX351242B (es) Un metodo para analizar una muestra de sangre de un sujeto por la presencia de un marcador de enfermedad.
MX2019013309A (es) Animales no humanos deficientes en lncrna.
MX2017008553A (es) Pcr cuantitativa multiple.
WO2018096375A3 (en) Determination of the receptive status of the endometrium
WO2016014595A3 (en) Antibodies that recognize red blood cell antigens
EP3931347A4 (en) IMPROVED METHOD FOR CLINICAL SAMPLE PREPARATION FOR NUCLEIC ACID AMPLIFICATION
NZ708061A (en) Methods for generating genetically superior animals
MA20150026A1 (fr) Cellules souches placentaires, procédés d'isolation de ces cellules et utilisation de ces procédés
MX2021011511A (es) Multiplexación de elementos reguladores para identificar elementos reguladores específicos al tipo de célula.