EA202191788A1 - POLYPEPTIDES USED FOR GENE EDITING AND METHODS OF THEIR APPLICATION - Google Patents
POLYPEPTIDES USED FOR GENE EDITING AND METHODS OF THEIR APPLICATIONInfo
- Publication number
- EA202191788A1 EA202191788A1 EA202191788A EA202191788A EA202191788A1 EA 202191788 A1 EA202191788 A1 EA 202191788A1 EA 202191788 A EA202191788 A EA 202191788A EA 202191788 A EA202191788 A EA 202191788A EA 202191788 A1 EA202191788 A1 EA 202191788A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- interest
- target sequence
- rna
- compositions
- nucleic acid
- Prior art date
Links
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- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
В изобретении описывают композиции и способы связывания с представляющей интерес целевой последовательностью. Композиции включают слитые белки между ДНК-связывающими белками или белковыми доменами и модифицирующими нуклеиновую кислоту белками или белковыми доменами. Композиции применяют при расщеплении или модификации представляющей интерес целевой последовательности, визуализации представляющей интерес целевой последовательности и модификации экспрессии исследуемой целевой последовательности. Композиции содержат полипептиды РНК-направляемых нуклеаз, РНК CRISPR, транс-активирующие РНК CRISPR, направляющие РНК, дезаминазы и кодирующие их молекулы нуклеиновых кислот. Также предусматривают векторы и клетки-хозяева, содержащие молекулы нуклеиновой кислоты. Кроме того, предусматривают системы CRISPR для связывания представляющей интерес целевой последовательности, причем система CRISPR включает полипептид РНК-направляемой нуклеазы и одну или несколько направляющих РНК. Также предусматривают дезаминазы, которые могут быть слиты с ДНК-связывающим полипептидом и могут быть полезны для редактирования генов.The invention describes compositions and methods of linking to a target sequence of interest. The compositions include fusion proteins between DNA binding proteins or protein domains and nucleic acid modifying proteins or protein domains. The compositions are useful in cleaving or modifying the target sequence of interest, rendering the target sequence of interest, and modifying the expression of the target sequence of interest. The compositions contain RNA targeting nuclease polypeptides, CRISPR RNA, trans-activating CRISPR RNA, targeting RNA, deaminases and nucleic acid molecules encoding them. Also contemplated are vectors and host cells containing nucleic acid molecules. In addition, CRISPR systems are provided for binding a target sequence of interest, the CRISPR system comprising an RNA targeting nuclease polypeptide and one or more targeting RNAs. Also contemplated are deaminases that can be fused to a DNA-binding polypeptide and can be useful for gene editing.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962932169P | 2019-11-07 | 2019-11-07 | |
| PCT/US2019/068079 WO2020139783A2 (en) | 2018-12-27 | 2019-12-20 | Polypeptides useful for gene editing and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA202191788A1 true EA202191788A1 (en) | 2021-10-21 |
Family
ID=78572588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA202191788A EA202191788A1 (en) | 2019-11-07 | 2019-12-20 | POLYPEPTIDES USED FOR GENE EDITING AND METHODS OF THEIR APPLICATION |
Country Status (1)
| Country | Link |
|---|---|
| EA (1) | EA202191788A1 (en) |
-
2019
- 2019-12-20 EA EA202191788A patent/EA202191788A1/en unknown
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