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Publication of AR117535A1publicationCriticalpatent/AR117535A1/en
Se proporcionan composiciones y métodos de unión con una secuencia diana de interés. Las composiciones incluyen proteínas de fusión entre proteínas de unión a ADN o dominios de proteína y proteínas que modifican ácidos nucleicos o dominios de proteínas. Las composiciones se usan en la escisión o la modificación de una secuencia diana de interés, visualización de una secuencia diana de interés y la modificación de la expresión de una secuencia de interés. Las composiciones comprenden polipéptidos de nucleasa guiados por ARN, ARN de CRISPR, ARN de CRISPR transactivantes, ARN de guía, desaminasas y moléculas de ácido nucleico que los codifican. También se proporcionan vectores y células huésped que comprenden las moléculas de ácido nucleico. También se proporcionan sistema de CRISPR para unir una secuencia diana de interés, en donde el sistema de CRISPR comprende un polipéptido de nucleasa guiado por ARN y uno o más ARN de guía. También se proporcionan desaminasas que se pueden fusionar con un polipéptido de unión a ADN y también pueden ser de utilidad para la edición de genes.Compositions and methods of binding with a target sequence of interest are provided. The compositions include fusion proteins between DNA-binding proteins or protein domains and proteins that modify nucleic acids or protein domains. The compositions are used in the cleavage or modification of a target sequence of interest, visualization of a target sequence of interest, and modification of the expression of a sequence of interest. The compositions comprise RNA-guided nuclease polypeptides, CRISPR RNA, transactivating CRISPR RNA, guide RNA, deaminases, and nucleic acid molecules that encode them. Vectors and host cells comprising the nucleic acid molecules are also provided. Also provided are CRISPR system for linking a target sequence of interest, wherein the CRISPR system comprises an RNA-guided nuclease polypeptide and one or more guide RNAs. Also provided are deaminases that can be fused to a DNA-binding polypeptide and can also be useful for gene editing.
ARP190103890A2018-12-272019-12-26
USEFUL POLYPEPTIDES FOR EDITING GENES AND METHODS OF USE
AR117535A1
(en)
A prevotella and francisella crispr protein 1 (cpf1) isolated from lachnospiraceae bacterium nd2006; fusion protein comprising it; nucleic acid; vector; methods for altering the genome of a cell or a double-stranded DNA molecule in vitro using such proteins (divisional de sol. 201903004)
Composition for cleaving a target DNA comprising a specific guide RNA for the target DNA and the nucleic acid encoding the Cas protein or the Cas protein itself, and its uses
METHODS OF PRODUCTION OF A PROTEIN OF INTEREST HETEROLOGY IN A CELL, TO INCREASE THE SPECIFIC CELLULAR PRODUCTIVITY OF A PROTEIN OF MEMBRANE INTEREST OR SECRETED IN A CELL, TO INCREASE THE EFFECTIVENESS OF TRANSFECTION OF A CELL THAT EXPRESSES A PROTEIN OF INTEREST SECRETED IN A CELL AND TO IDENTIFY A PROTEIN FUNCTION MODULATOR WITH START DOMAIN, EXPRESSION VECTORS, CELLS, PROTEINS, PHARMACEUTICAL COMPOSITIONS AND ITS USES
Fusion protein, nucleic acid molecule, method for editing genetic material, and, systems for editing genetic material and for delivering genome editing components