EA201891697A1 - Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк - Google Patents

Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк

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EA201891697A1
EA201891697A1 EA201891697A EA201891697A EA201891697A1 EA 201891697 A1 EA201891697 A1 EA 201891697A1 EA 201891697 A EA201891697 A EA 201891697A EA 201891697 A EA201891697 A EA 201891697A EA 201891697 A1 EA201891697 A1 EA 201891697A1
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nucleic acid
rna
dna
decoding
terminal region
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EA201891697A
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EA037304B1 (ru
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Ясухиро Муракава
Юдзиро Такегами
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КАБУСИКИ КАЙСЯ ДиЭнЭйФОРМ
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Publication of EA201891697A1 publication Critical patent/EA201891697A1/ru
Publication of EA037304B1 publication Critical patent/EA037304B1/ru

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Изобретение предлагает способ анализа, который позволяет идентифицировать элемент ДНК с повышенной чувствительностью и определить уровень транскрипции элемента ДНК. Настоящее изобретение относится к способу декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку РНК, включающему стадию получения-декодирования способа получения с использованием матричной РНК по меньшей мере одной нуклеиновой кислоты, выбранной из группы, состоящей из нуклеиновой кислоты РНК 5'-концевого участка матричной РНК или комплементарной нуклеиновой кислоты ДНК, соответствующей 5'-концевому участку матричной РНК; нуклеиновой кислоты РНК 3'-концевого участка матричной РНК или комплементарной нуклеиновой кислоты ДНК, соответствующей 3'-концевому участку матричной РНК; нуклеиновой кислоты РНК частичного участка антисмысловой цепи или смысловой цепи полноразмерной матричной РНК; и их сочетаний, или нуклеиновой кислоты, последовательность которой содержит две или более связанных последовательностей, где матричная РНК, используемая на стадии получения-декодирования, представляет собой растущую цепь РНК. Элемент ДНК можно идентифицировать и проанализировать с повышенной точностью и повышенной чувствительностью путем определения информации о декодированной последовательности нуклеиновой кислоты и картирования элемента ДНК в геноме.
EA201891697A 2016-01-27 2017-01-13 Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк EA037304B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016013575 2016-01-27
PCT/JP2017/001097 WO2017130750A1 (ja) 2016-01-27 2017-01-13 Ｒｎａの末端領域に対応する核酸の塩基配列を解読する方法およびｄｎａエレメントの分析方法

Publications (2)

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EA201891697A1 true EA201891697A1 (ru) 2019-01-31
EA037304B1 EA037304B1 (ru) 2021-03-09

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EA201891697A EA037304B1 (ru) 2016-01-27 2017-01-13 Способ декодирования последовательности оснований нуклеиновой кислоты, соответствующей концевому участку рнк, и способ анализа элемента днк

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US (1) US11312991B2 (ru)
EP (1) EP3409773B1 (ru)
JP (1) JP6417603B2 (ru)
DK (1) DK3409773T3 (ru)
EA (1) EA037304B1 (ru)
WO (1) WO2017130750A1 (ru)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6280935B1 (en) 1994-10-13 2001-08-28 Lynx Therapeutics, Inc. Method of detecting the presence or absence of a plurality of target sequences using oligonucleotide tags
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
FR2733762B1 (fr) 1995-05-02 1997-08-01 Genset Sa Methode de couplage specifique de la coiffe de l'extremite 5' d'un fragment d'arnm et preparation d'arnm et d'adnc complet
US5962271A (en) 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US6013488A (en) 1996-07-25 2000-01-11 The Institute Of Physical And Chemical Research Method for reverse transcription
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
EP1197552B1 (en) 2000-08-25 2006-11-08 Riken Method of preparing normalized and/or subtracted cDNA
US20050250100A1 (en) 2002-06-12 2005-11-10 Yoshihide Hayashizaki Method of utilizing the 5'end of transcribed nucleic acid regions for cloning and analysis
JP4403069B2 (ja) 2002-08-12 2010-01-20 独立行政法人理化学研究所 クローニングおよび分析のためのｍＲＮＡの５’末端の使用方法
JP2009072062A (ja) 2006-04-07 2009-04-09 Institute Of Physical & Chemical Research 核酸の５’末端を単離するための方法およびその適用
CA2855368A1 (en) * 2011-11-11 2013-05-16 Millennium Pharmaceuticals, Inc. Biomarkers of response to proteasome inhibitors
US9333578B2 (en) 2014-06-30 2016-05-10 General Electric Company Fiber reinforced brazed components and methods

Also Published As

Publication number Publication date
EP3409773A1 (en) 2018-12-05
EP3409773B1 (en) 2020-07-29
DK3409773T3 (da) 2020-09-07
EA037304B1 (ru) 2021-03-09
US20190032127A1 (en) 2019-01-31
US11312991B2 (en) 2022-04-26
WO2017130750A1 (ja) 2017-08-03
EP3409773A4 (en) 2019-07-03
JP6417603B2 (ja) 2018-11-07
JPWO2017130750A1 (ja) 2018-02-01

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