EA201691550A1 - Промоторы, подходящие для экспрессии гетерологичных генов в дрожжах - Google Patents

Промоторы, подходящие для экспрессии гетерологичных генов в дрожжах

Info

Publication number
EA201691550A1
EA201691550A1 EA201691550A EA201691550A EA201691550A1 EA 201691550 A1 EA201691550 A1 EA 201691550A1 EA 201691550 A EA201691550 A EA 201691550A EA 201691550 A EA201691550 A EA 201691550A EA 201691550 A1 EA201691550 A1 EA 201691550A1
Authority
EA
Eurasian Patent Office
Prior art keywords
expression
yeast
genes
heterological
promotors
Prior art date
Application number
EA201691550A
Other languages
English (en)
Other versions
EA035512B1 (ru
Inventor
Джессика Лей Мак Грэт
Бастьен Шеврё
Питер Скотт Йорги
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201691550A1 publication Critical patent/EA201691550A1/ru
Publication of EA035512B1 publication Critical patent/EA035512B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к применению новых промоторов для экспрессии гетерологичных генов, предпочтительно для экспрессии генов в организмах рода Yarrowia, к генетически модифицированным организмам рода Yarrowia и к способу получения биосинтетических продуктов культивированием генетически модифицированных организмов.
EA201691550A 2014-01-31 2015-01-29 Промоторы, подходящие для экспрессии гетерологичных генов в дрожжах EA035512B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461933979P 2014-01-31 2014-01-31
PCT/US2015/013453 WO2015116781A2 (en) 2014-01-31 2015-01-29 Promoters suitable for heterologous gene expression in yeast

Publications (2)

Publication Number Publication Date
EA201691550A1 true EA201691550A1 (ru) 2016-11-30
EA035512B1 EA035512B1 (ru) 2020-06-29

Family

ID=53757913

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201691550A EA035512B1 (ru) 2014-01-31 2015-01-29 Промоторы, подходящие для экспрессии гетерологичных генов в дрожжах

Country Status (6)

Country Link
US (1) US10611807B2 (ru)
EP (1) EP3102683B1 (ru)
CN (1) CN106414744B (ru)
BR (1) BR112016017468A2 (ru)
EA (1) EA035512B1 (ru)
WO (1) WO2015116781A2 (ru)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2018138966A1 (ja) * 2017-01-27 2018-08-02 神戸天然物化学株式会社 形質転換体およびその利用
JP2020535794A (ja) 2017-09-25 2020-12-10 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． トランス−レチナールの生産

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4937189A (en) 1985-10-18 1990-06-26 Pfizer Inc. Expression and secretion of heterologous proteins by Yarrowia lipolytica transformants
AU2887897A (en) 1996-05-21 1997-12-09 Novo Nordisk A/S Novel yeast promoters suitable for expression cloning in yeast and heterologous expression of proteins in yeast
US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
US7064196B2 (en) 2003-05-20 2006-06-20 E. I. Du Pont De Nemours And Company Genes encoding carotenoid compounds
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
UA94038C2 (ru) * 2005-03-18 2011-04-11 Майкробиа, Инк. Продуцирование каротиноидов в маслянистых дрожжах и грибах
EP1866233B1 (en) 2005-03-23 2012-04-04 Autoliv Asp, Inc. Airbag tether release
WO2008042338A2 (en) 2006-09-28 2008-04-10 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
US8846374B2 (en) * 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
US10472661B2 (en) * 2014-10-08 2019-11-12 Dsm Ip Assets B.V. Method for increasing glycosylation of a composition comprising steviol glycosides

Also Published As

Publication number Publication date
CN106414744A (zh) 2017-02-15
US20160333058A1 (en) 2016-11-17
BR112016017468A2 (pt) 2017-10-10
EP3102683A2 (en) 2016-12-14
WO2015116781A2 (en) 2015-08-06
US10611807B2 (en) 2020-04-07
EP3102683A4 (en) 2017-07-05
EA035512B1 (ru) 2020-06-29
EP3102683B1 (en) 2019-10-23
WO2015116781A3 (en) 2015-11-19
CN106414744B (zh) 2020-11-06

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG TJ TM RU