EA201300486A1 - Способ быстрой лабораторной диагностики заболеваний, основанный на выявлении специфических белков - Google Patents
Способ быстрой лабораторной диагностики заболеваний, основанный на выявлении специфических белковInfo
- Publication number
- EA201300486A1 EA201300486A1 EA201300486A EA201300486A EA201300486A1 EA 201300486 A1 EA201300486 A1 EA 201300486A1 EA 201300486 A EA201300486 A EA 201300486A EA 201300486 A EA201300486 A EA 201300486A EA 201300486 A1 EA201300486 A1 EA 201300486A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- diseases
- specific
- proteins
- test systems
- identification
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Изобретение может быть использовано в медицине и ветеринарии для создания тест-систем для диагностики различных заболеваний, маркерами которых являются уникальные белки или изменение их концентрации. Суть изобретения: метод быстрой лабораторной диагностики заболеваний, основанный на выявлении характерных для заболевания специфических белков-мишеней в реакции их специфического взаимодействия с другими белками-реагентами, отличающийся тем, что берут специфический для данного заболевания белок-мишень, гидролизуют протеолитическими ферментами, модифицируют химическую структуру образовавшейся композиции олигопептидов таким образом, чтобы их заряд изменился на противоположный, и используют далее уже в качестве белков-реагентов вместо специфических иммуноглобулинов в методах иммунофлюоресценции и иммуноферментном методе. Метод может быть применен для выявления вирусных антигенов, микробных антигенов в различных биологических жидкостях человека, также метод может быть использован для детекции новых и малоизученных инфекционных заболеваний в связи с простотой получения диагностикумов. Метод позволяет исключить этап получения специфических иммуноглобулинов и соответственно отказаться от использования животных, ускорить до нескольких дней разработку тест-систем для новых и малоизученных инфекционных заболеваний, значительно удешевить стоимость производства тест-систем при той же чувствительности метода при значительно большей его специфичности, метод коррелирует по специфичности и чувствительности с культуральным методом выделения вируса и полимеразной цепной реакцией, не требует
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/RU2010/000689 WO2012070963A1 (ru) | 2010-11-22 | 2010-11-22 | Способ быстрой лабораторной диагностики заболеваний, основанный на выявлении специфических белков и устройство для его осуществления |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201300486A1 true EA201300486A1 (ru) | 2013-11-29 |
| EA024771B1 EA024771B1 (ru) | 2016-10-31 |
Family
ID=46064699
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201300486A EA024771B1 (ru) | 2010-11-22 | 2010-11-22 | Способ быстрой лабораторной диагностики заболеваний, основанный на выявлении специфических белков |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US20120129195A1 (ru) |
| EA (1) | EA024771B1 (ru) |
| WO (1) | WO2012070963A1 (ru) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111480079A (zh) * | 2017-10-16 | 2020-07-31 | 伊纳诺生物股份有限公司 | 疾病蛋白质组蛋白质阵列及其应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5567301A (en) * | 1995-03-01 | 1996-10-22 | Illinois Institute Of Technology | Antibody covalently bound film immunobiosensor |
| KR20070094668A (ko) * | 2003-12-23 | 2007-09-20 | 칼리퍼 라이프 사이언시즈, 인크. | 분석물 주입 시스템 |
| US9958442B2 (en) * | 2009-02-11 | 2018-05-01 | Duke University | Sensors incorporating antibodies and methods of making and using the same |
-
2010
- 2010-11-22 EA EA201300486A patent/EA024771B1/ru not_active IP Right Cessation
- 2010-11-22 WO PCT/RU2010/000689 patent/WO2012070963A1/ru active Application Filing
-
2011
- 2011-02-01 US US12/931,459 patent/US20120129195A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012070963A1 (ru) | 2012-05-31 |
| EA024771B1 (ru) | 2016-10-31 |
| US20120129195A1 (en) | 2012-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Najjar et al. | A lab-on-a-chip for the concurrent electrochemical detection of SARS-CoV-2 RNA and anti-SARS-CoV-2 antibodies in saliva and plasma | |
| Tseng et al. | Integrated microfluidic system for rapid detection of influenza H1N1 virus using a sandwich-based aptamer assay | |
| Al Johani et al. | MERS-CoV diagnosis: an update | |
| Zaghloul et al. | Recombinase polymerase amplification as a promising tool in hepatitis C virus diagnosis | |
| Klaus et al. | Detection and genome sequencing of SARS-CoV-2 in a domestic cat with respiratory signs in Switzerland | |
| Daniels et al. | Laboratory diagnosis of Nipahand Hendra virus infections | |
| Rong et al. | COVID-19 diagnostic methods and detection techniques | |
| Deb et al. | Trends in veterinary diagnostics | |
| Rémond et al. | Diagnosis and screening of foot-and-mouth disease | |
| Wang et al. | Rapid detection of foot-and-mouth disease virus using reverse transcription recombinase polymerase amplification combined with a lateral flow dipstick | |
| Stranieri et al. | Reverse transcriptase loop-mediated isothermal amplification for the detection of feline coronavirus | |
| CN104726606B (zh) | 一种pcr酶联双杂交法检测病原微生物检测方法 | |
| Donia et al. | Integration of RT-LAMP and microfluidic technology for detection of SARS-CoV-2 in wastewater as an advanced point-of-care platform | |
| Veir et al. | Molecular diagnostic assays for infectious diseases in cats | |
| CN104372072A (zh) | 一种通用型检测血小板细菌污染的荧光定量pcr方法 | |
| EA201300486A1 (ru) | Способ быстрой лабораторной диагностики заболеваний, основанный на выявлении специфических белков | |
| Malik et al. | Biotechnological innovations in farm and pet animal disease diagnosis | |
| CN105759038A (zh) | 一种检测生物样品中结核分枝杆菌的免疫检测方法及试剂盒 | |
| Ullah et al. | Detection of Peste des Petits Ruminants viral RNA in fecal samples of goats after an outbreak in Punjab Province of Pakistan: A longitudinal study | |
| Mahmood et al. | Application of serum based PCR and fluorescence polarization assay for diagnosis of brucellosis among people occupationally at risk to disease | |
| El-Tholoth et al. | Molecular characterization and developing a point-of-need molecular test for diagnosis of bovine papillomavirus (BPV) Type 1 in cattle from Egypt | |
| Najjar et al. | Lab-on-a-chip multiplexed electrochemical sensor enables simultaneous detection of SARS-CoV-2 RNA and host antibodies | |
| Feyisa et al. | Review on Pen-Side Tests: Prospects in Animal Disease Diagnosis and Eradication Program | |
| Zandi et al. | Biosensor-based methods for Crimean-Congo hemorrhagic fever virus detection | |
| Fisher et al. | SARS-CoV-2 viral replication in a high throughput human primary epithelial airway organ model |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM BY KG MD TJ TM |