EA200901176A1 - Способ обработки наборов флуоресцентных изображений и устройство для его осуществления - Google Patents

Способ обработки наборов флуоресцентных изображений и устройство для его осуществления

Info

Publication number
EA200901176A1
EA200901176A1 EA200901176A EA200901176A EA200901176A1 EA 200901176 A1 EA200901176 A1 EA 200901176A1 EA 200901176 A EA200901176 A EA 200901176A EA 200901176 A EA200901176 A EA 200901176A EA 200901176 A1 EA200901176 A1 EA 200901176A1
Authority
EA
Eurasian Patent Office
Prior art keywords
partial images
image
implementation
threshold value
produced
Prior art date
Application number
EA200901176A
Other languages
English (en)
Other versions
EA015558B1 (ru
Inventor
Михаэль Томс
Original Assignee
Дюрр Денталь Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дюрр Денталь Аг filed Critical Дюрр Денталь Аг
Publication of EA200901176A1 publication Critical patent/EA200901176A1/ru
Publication of EA015558B1 publication Critical patent/EA015558B1/ru

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
    • A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
    • A61B5/0088—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for oral or dental tissue
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dentistry (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Medical Informatics (AREA)
  • Audiology, Speech & Language Pathology (AREA)
  • Surgery (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Processing (AREA)
  • Image Analysis (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)

Abstract

В заявке описаны способ и устройство, предназначенные для обработки флуоресцентных частичных изображений, представляющих одну и ту же область объекта, и работающие независимо от постороннего света. С помощью камеры (10) создают два частичных изображения объекта в красном и зеленом цветах. Из этих двух частичных изображений попиксельно формируют изображение-частное, и для этого изображения-частного определяют, с какой частотой встречаются точки изображения, имеющие заданное отношение красного и зеленого цветов. Для полученной таким образом кривой распределения определяют среднее значение и ширину. Эти две конечные величины кривой распределения используют для вычисления порогового значения. Затем с применением этого порогового значения модифицируют изображение-частное таким образом, чтобы повысить контраст в отношении интересующих деталей.
EA200901176A 2007-03-17 2008-02-28 Способ обработки наборов флуоресцентных изображений и устройство для его осуществления EA015558B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007014413.1A DE102007014413B4 (de) 2007-03-17 2007-03-17 Verfahren zum Auswerten von Fluoreszenzbildsätzen und Vorrichtung zu seiner Durchführung
PCT/EP2008/001567 WO2008113461A1 (de) 2007-03-17 2008-02-28 Verfahren und vorrichtung zum auswerten von fluoreszenzbildsätzen und vorrichtung zu seiner durchführung

Publications (2)

Publication Number Publication Date
EA200901176A1 true EA200901176A1 (ru) 2010-04-30
EA015558B1 EA015558B1 (ru) 2011-08-30

Family

ID=39645692

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200901176A EA015558B1 (ru) 2007-03-17 2008-02-28 Способ обработки наборов флуоресцентных изображений и устройство для его осуществления

Country Status (10)

Country Link
US (1) US8363912B2 (ru)
EP (1) EP2120682B1 (ru)
JP (1) JP5192499B2 (ru)
CN (1) CN101636106B (ru)
AT (1) ATE489891T1 (ru)
CA (1) CA2691555C (ru)
DE (2) DE102007014413B4 (ru)
EA (1) EA015558B1 (ru)
ES (1) ES2356301T3 (ru)
WO (1) WO2008113461A1 (ru)

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* Cited by examiner, † Cited by third party
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DE102004024494B4 (de) * 2004-05-16 2019-10-17 Dürr Dental SE Medizinische Kamera
US20120263646A1 (en) 2009-10-15 2012-10-18 Guerbet Imaging agents and their use for the diagnostic in vivo of neurodegenerative diseases, notably alzheimer's disease and derivative diseases
JP5540952B2 (ja) * 2010-07-09 2014-07-02 ソニー株式会社 蛍光強度補正方法及び蛍光強度算出装置
KR101709058B1 (ko) * 2015-02-09 2017-02-22 연세대학교 산학협력단 구취 진단을 위한 정보제공방법
JP6721939B2 (ja) * 2016-03-25 2020-07-15 株式会社松風 蛍光画像解析装置
ES2886082T3 (es) 2016-04-13 2021-12-16 Inspektor Res Systems B V Examen dental bifrecuencia
TWI667999B (zh) 2018-05-17 2019-08-11 廣達電腦股份有限公司 動態調整螢光成像的方法及裝置

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US3657537A (en) * 1970-04-03 1972-04-18 Bausch & Lomb Computerized slit-scan cyto-fluorometer for automated cell recognition
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4704891A (en) * 1986-08-29 1987-11-10 Becton, Dickinson And Company Method and materials for calibrating flow cytometers and other analysis instruments
CA2042075C (en) * 1991-05-08 2001-01-23 Branko Palcic Endoscopic imaging system
US5834203A (en) * 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
EP1169722A1 (en) * 1999-03-18 2002-01-09 Cambridge Research & Instrumentation, Inc. High-efficiency multiple probe imaging system
DE19915137C2 (de) * 1999-03-26 2001-10-18 Michael Schaefer Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens
JP2001017379A (ja) * 1999-07-09 2001-01-23 Fuji Photo Film Co Ltd 蛍光診断装置
JP4245787B2 (ja) * 1999-09-29 2009-04-02 富士フイルム株式会社 蛍光画像取得方法および装置
JP2001314366A (ja) * 2000-05-08 2001-11-13 Fuji Photo Film Co Ltd 蛍光画像表示方法および装置
US6687000B1 (en) * 2000-06-26 2004-02-03 Wisconsin Alumni Research Foundation Photon-sorting spectroscopic microscope system
DE10207918A1 (de) * 2002-02-23 2003-09-11 Nanotype Gmbh Verfahren zum Nachweis von Mutationen
DE10319946A1 (de) * 2003-05-02 2004-11-18 Leica Microsystems Heidelberg Gmbh Verfahren zur Klassifikation von Objektbildbereichen eines mit einem vorzugsweise konfokalen Rastermikroskop zu detekierenden Objekts
SE0301945D0 (sv) * 2003-06-30 2003-06-30 Gyros Ab Confidence determination
DE10339312A1 (de) * 2003-08-27 2005-03-31 Leica Microsystems Heidelberg Gmbh Verfahren zur Trennung von Fluoreszenzspektren von in einer Probe vorhandenen Farbstoffen
DE10344140A1 (de) * 2003-09-24 2005-04-21 Zeiss Carl Jena Gmbh Verfahren zur Bewertung der Qualität von Lumineszenzmessungen
JP4517633B2 (ja) * 2003-11-25 2010-08-04 ソニー株式会社 対象物検出装置及び方法
JP2005308504A (ja) * 2004-04-21 2005-11-04 Yokogawa Electric Corp バイオチップ測定方法およびバイオチップ読取装置
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Also Published As

Publication number Publication date
CN101636106B (zh) 2013-03-20
DE102007014413B4 (de) 2016-02-04
US8363912B2 (en) 2013-01-29
CN101636106A (zh) 2010-01-27
WO2008113461A1 (de) 2008-09-25
CA2691555A1 (en) 2008-09-25
EP2120682A1 (de) 2009-11-25
DE502008001939D1 (de) 2011-01-13
CA2691555C (en) 2016-04-26
DE102007014413A1 (de) 2008-09-18
US20100142777A1 (en) 2010-06-10
ATE489891T1 (de) 2010-12-15
ES2356301T3 (es) 2011-04-06
JP5192499B2 (ja) 2013-05-08
JP2010521687A (ja) 2010-06-24
EP2120682B1 (de) 2010-12-01
EA015558B1 (ru) 2011-08-30

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Designated state(s): AM AZ BY KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): KZ

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU