DE10344140A1 - Verfahren zur Bewertung der Qualität von Lumineszenzmessungen - Google Patents

Verfahren zur Bewertung der Qualität von Lumineszenzmessungen Download PDF

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Publication number
DE10344140A1
DE10344140A1 DE2003144140 DE10344140A DE10344140A1 DE 10344140 A1 DE10344140 A1 DE 10344140A1 DE 2003144140 DE2003144140 DE 2003144140 DE 10344140 A DE10344140 A DE 10344140A DE 10344140 A1 DE10344140 A1 DE 10344140A1
Authority
DE
Germany
Prior art keywords
area
value
measurements
luminescence
enriched
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE2003144140
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English (en)
Inventor
Peter Westphal
Rainer Dittrich
Tobias Neumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jenoptik AG
Original Assignee
Carl Zeiss Jena GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Jena GmbH filed Critical Carl Zeiss Jena GmbH
Priority to DE2003144140 priority Critical patent/DE10344140A1/de
Publication of DE10344140A1 publication Critical patent/DE10344140A1/de
Withdrawn legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Bewertung der Qualität von Messungen der Lumineszenz von Proben. DOLLAR A Bei einem solchen Verfahren werden eine Anzahl von (M >= 1) Messungen (L¶m¶) mit m = 1, ...M durchgeführt, bei jeder Messung (L¶m¶) die Intensitätswerte elektromagnetischer Strahlung von Orten in mindestens einem ersten, mit lumineszentem Material angereicherten Gebiet (A¶s¶) der Probe und von Orten in mindestens einem zweiten, nicht mit lumineszentem Material angereicherten Gebiet (A¶h¶) der Probe in Zuordnung zu den Orten gemessen und registriert, Mittelwerte (mu·m·¶s¶) und (mu·m·¶h¶) sowie Standardabweichungen (sigma·m·¶s¶) und (sigma·m·¶h¶) von den den Orten im ersten Bereich (A¶s¶) beziehungsweise im zweiten Bereich (A¶h¶) zugeordneten Intensitätswerten bestimmt, und die Mittelwerte (mu·m·¶s¶, mu·m·¶h¶) und/oder Standardabweichungen (sigma·m·¶s¶, sigma·m·¶h¶) zu Bewertungskriterien verknüpft, anhand derer die Qualität bewertet wird.
DE2003144140 2003-09-24 2003-09-24 Verfahren zur Bewertung der Qualität von Lumineszenzmessungen Withdrawn DE10344140A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE2003144140 DE10344140A1 (de) 2003-09-24 2003-09-24 Verfahren zur Bewertung der Qualität von Lumineszenzmessungen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2003144140 DE10344140A1 (de) 2003-09-24 2003-09-24 Verfahren zur Bewertung der Qualität von Lumineszenzmessungen

Publications (1)

Publication Number Publication Date
DE10344140A1 true DE10344140A1 (de) 2005-04-21

Family

ID=34353039

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2003144140 Withdrawn DE10344140A1 (de) 2003-09-24 2003-09-24 Verfahren zur Bewertung der Qualität von Lumineszenzmessungen

Country Status (1)

Country Link
DE (1) DE10344140A1 (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3000209A1 (fr) * 2012-12-26 2014-06-27 Biomerieux Sa Procede et systeme pour detecter et mesurer des signaux de fluorescence
DE102007014413B4 (de) * 2007-03-17 2016-02-04 DüRR DENTAL AG Verfahren zum Auswerten von Fluoreszenzbildsätzen und Vorrichtung zu seiner Durchführung

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2902776A1 (de) * 1978-01-25 1979-08-02 Kyoto Daiichi Kagaku Kk Verfahren fuer das analysieren mit farbidentifizierungs-testpapier und eine vorrichtung zur durchfuehrung des verfahrens
GB2335978A (en) * 1998-03-30 1999-10-06 Cambridge Imaging Ltd Detecting the Background Noise of a Biomedical Assay
WO2001055699A1 (en) * 2000-01-27 2001-08-02 Pe Corporation (Ny) Methods and apparatus for performing spectral calibration
WO2002077620A1 (de) * 2001-03-28 2002-10-03 Clondiag Chip Technologies Gmbh Vorrichtung zur referenzierung von fluoreszenzsignalen
DE10200865A1 (de) * 2001-03-28 2002-10-10 Clondiag Chip Tech Gmbh Vorrichtung zur Referenzierung von Fluoreszenzsignalen
EP1338888A1 (de) * 2000-08-31 2003-08-27 pe s Gesellschaft für medizinische Diagnosesysteme mbH Verfahren zur Verbesserung der Messgenauigkeit bei Sensoren, insbesondere Bio-Sensoren, die zur Bestimmung der Signale Fluoreszenzstrahlung verwenden

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2902776A1 (de) * 1978-01-25 1979-08-02 Kyoto Daiichi Kagaku Kk Verfahren fuer das analysieren mit farbidentifizierungs-testpapier und eine vorrichtung zur durchfuehrung des verfahrens
GB2335978A (en) * 1998-03-30 1999-10-06 Cambridge Imaging Ltd Detecting the Background Noise of a Biomedical Assay
WO2001055699A1 (en) * 2000-01-27 2001-08-02 Pe Corporation (Ny) Methods and apparatus for performing spectral calibration
EP1338888A1 (de) * 2000-08-31 2003-08-27 pe s Gesellschaft für medizinische Diagnosesysteme mbH Verfahren zur Verbesserung der Messgenauigkeit bei Sensoren, insbesondere Bio-Sensoren, die zur Bestimmung der Signale Fluoreszenzstrahlung verwenden
WO2002077620A1 (de) * 2001-03-28 2002-10-03 Clondiag Chip Technologies Gmbh Vorrichtung zur referenzierung von fluoreszenzsignalen
DE10200865A1 (de) * 2001-03-28 2002-10-10 Clondiag Chip Tech Gmbh Vorrichtung zur Referenzierung von Fluoreszenzsignalen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BROWN,Carl S., et.al.: Image metrics in the statistical analysis of DNA microarray data. In: PNAS, July 31, 2001, Vol.98, No.16, S.8944-8949 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007014413B4 (de) * 2007-03-17 2016-02-04 DüRR DENTAL AG Verfahren zum Auswerten von Fluoreszenzbildsätzen und Vorrichtung zu seiner Durchführung
FR3000209A1 (fr) * 2012-12-26 2014-06-27 Biomerieux Sa Procede et systeme pour detecter et mesurer des signaux de fluorescence
WO2014102502A1 (fr) * 2012-12-26 2014-07-03 bioMérieux Procede et systeme pour detecter et mesurer des signaux de fluorescence
US10168284B2 (en) 2012-12-26 2019-01-01 Biomerieux Method and system for detecting and measuring fluorescence signals
US10746662B2 (en) 2012-12-26 2020-08-18 Biomerieux Method and system for detecting and measuring fluorescence signals

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Legal Events

Date Code Title Description
OM8 Search report available as to paragraph 43 lit. 1 sentence 1 patent law
8181 Inventor (new situation)

Inventor name: DITTRICH, RAINER, DR., 07745 JENA, DE

Inventor name: NEUMANN, TOBIAS, DR., 07745 JENA, DE

Inventor name: WESTPHAL, PETER, DR., 07743 JENA, DE

8110 Request for examination paragraph 44
R119 Application deemed withdrawn, or ip right lapsed, due to non-payment of renewal fee

Effective date: 20140401