EA044377B1 - FEED ADDITIVES TO FEED FOR BROILER CHICKENS (OPTIONS), METHOD FOR PRODUCING FEED ADDITIVES TO FEED FOR BROILER CHICKENS - Google Patents

Description

The invention relates to feed production, in particular to a feed product for broiler chickens.

The use of curcumin, rutin, and polysorbates in medicine is known.

For example, the anti-inflammatory effect of a curcumin composition in combination with a mixture of tocopherols is known (CA 2595860 A1, formula, claims 1, 2).

From patent RU 2615815 C2 it is known to use the emulsifiers polysorbate 80, polysorbate 20, a mixture of polysorbate 20 and polysorbate 80 to obtain the dosage form.

A known composition includes curcumin, rutin, thioctic acid, which has antioxidant, immunomodulatory, anti-inflammatory effects (US 10980791 B1).

The use of curcumin, rutin, and polysorbate 80 in the production of feed additives for broiler chickens is not known from the prior art.

A group of inventions is proposed, including feed additives for feed products for broiler chickens and methods for their production.

One feed additive option for broiler chicken feed includes: amorphous mesoporous silica: 64 wt.%, curcumin and rutin solution in polysorbate 80: 36 wt.%.

In a particular case, the size of amorphous silica mesopores is from 0.2 to 1.8 nm, and a solution of curcumin and rutin in polysorbate 80 has the following composition:

curcumin 10.3 wt.%, rutin 3 wt.%, polysorbate 80 the rest.

Another option for feed additive to the feed product for broiler chickens includes: amorphous mesoporous silica: 44 wt.%, curcumin solution in polysorbate 80: 35 wt.%, activated carbon with cavity formations of 100 nm or more: 21 wt.%.

In a particular case, the size of amorphous silica mesopores is from 0.2 to 1.8 nm, and a solution of curcumin in polysorbate 80 has the following composition:

curcumin 10.3 wt.%, polysorbate 80 the rest.

The third option for a feed additive to a feed product for broiler chickens includes: amorphous mesoporous silicon dioxide: 44 wt.%, a solution of curcumin and rutin in polysorbate 80: 35 wt.%, activated carbon with cavity formations from 100 nm: 21 wt.%.

In a particular case, the size of amorphous silica mesopores is from 0.2 to 1.8 nm, and a solution of curcumin and rutin in polysorbate 80 has the following composition:

curcumin 10.3 wt.%, rutin 3 wt.%, polysorbate 80 the rest.

A method for producing a feed additive to a feed product for broiler chickens involves loading amorphous silicon dioxide or amorphous silicon dioxide mixed with activated carbon into a dispersant, stirring at a speed of from about 1000 to about 2000 rpm for about 0.5 hours with gradual adding micellized curcumin extract in polysorbate 80 or micellized curcumin and rutin extract in polysorbate 80, increasing the mixing speed to 7000-9000 rpm until a homogeneous product is obtained.

The technical result is an increase in growth rates and an improvement in the quality of meat of broiler chickens.

A feed product used in conjunction with the proposed feed additive can be presented, for example, as follows.

The feed product includes wheat, corn, soybean meal, limestone, salt, vegetable oil, lysine monochlorohydrate, methionine, threonine, feather protein concentrate (PPC), semi-defatted soybean, monocalcium phosphate, wheat bran, sodium sulfate, choline chloride.

It should be noted that the above feed is given as an example; the proposed feed additives can be used with any other feed for broiler chickens.

The feed additives disclosed in this group of inventions are suitable for the digestion of broiler chickens, ensure complete digestion of feed in the stomachs of chickens, and the resulting residues are decomposed by pancreatic amylase when passing through the intestinal tracts and have a synergistic effect with intestinal microorganisms, enhancing the decomposition of feed residues, improving immunity and quality of chicken meat. The feed additive is environmentally friendly, safe, no chemical residues, no pollution, no toxic or side effects. The proposed feed additive can significantly improve the growth and slaughter performance of broiler chickens, promote the growth of broiler chickens and improve the feed conversion ratio, regulate the microecological balance of the intestines of broiler chickens, increase the antioxidant capacity and immune function of 1 044377 broiler chickens, increase the body's resistance to diseases and resilience, obtain good economic benefits and promote the healthy and rapid development of livestock farming.

The preparation of additives is possible as follows.

Aerosil carrier was loaded into the IKA T 50 digital dispersant and, with stirring at about 1000 rpm, micellated curcumin extract in polysorbate 80 was gradually added over 0.5 hours; at room temperature, after adding the entire amount of extract, the stirring speed was increased to 7000 rpm to increase dispersibility and uniformity of distribution of the substance. At a ratio of 55 to 45%, a yellow powder was obtained, which was added to the main feed ration while mixing in a colloid mill.

Aerosil carrier was loaded into the IKA T 50 digital dispersant and, while stirring at about 1500 rpm, micellated extract of curcumin and rutin in polysorbate 80 was gradually added; after adding the entire amount of extract, the stirring speed was increased to 7000 rpm to increase dispersibility and uniformity of distribution of the substance. At a ratio of 64 to 36%, a yellow bulk product was obtained, which was added to the main feed ration while mixing in a colloid mill for a certain time.

In order to reduce the cost of the final product and reach an industrial scale, available carriers of active substrates, namely amorphous silicon dioxide with a nanoporous particle structure and pharmacopoeial activated carbon, were investigated and tested. It is known that the addition of silicon dioxide to the diets of broiler chickens increases the growth of broiler chickens at the end of growing (42 days) by 5.4%, reduces the cost of feed to obtain 1 kg of live weight gain by 4.8%, and the safety of livestock by 4 .9%, reduces the content of heavy metals in the muscle tissue homogenate, has a positive effect on the development of the intestinal microflora of meat chickens and the economic indicators of growing meat poultry.

An amorphous silicon dioxide carrier with a mesopore size of 0.2 to 1.8 nm was loaded into an IKA T 50 digital dispersant and, with stirring at about 1500 rpm, a micellized extract of curcumin and rutin in polysorbate 80 (curcumin 10) was gradually added over 0.5 hours , 3 wt.%, rutin 3 wt.%, the rest polysorbate 80), after adding the entire amount of the extract, the stirring speed was increased to 8000 rpm to increase dispersibility and uniformity of distribution of the substance. At a ratio of 64 to 36 wt.%, a yellow bulk product was obtained, which was included in the main diet of mixed feed when mixed in a colloid mill.

The following experiment describes the preparation of a supplement where activated carbon deposited with micellized curcumin acts as an additional carrier, since the literature indicates that the introduction of active carbon into the diet of broiler chickens helps to normalize the average hemoglobin content in the erythrocyte. Biochemical studies indicate a tendency towards normalization of indicators of the functional state of the liver of broiler chickens, as well as a beneficial effect of the active carbon feed additive on indicators of mineral metabolism, which is most likely due to the ash residue of the additive;

The IKA IKA T 50 digital dispersant is loaded with amorphous silicon dioxide and pharmacopoeial activated carbon, while stirring at about 2000 rpm, micellized curcumin extract in polysorbate 80 was gradually added over 0.5 hours, after adding the entire amount of extract, the stirring speed was increased to 9000 rpm /min to increase dispersibility and uniformity of distribution of the substance. To prepare the additive, 4.4 g of amorphous silicon dioxide (44 wt.%), 3.5 g (35 wt.%) of a solution of curcumin in polysorbate 80 (curcumin 10.3 wt.%, polysorbate 80 the rest) and 2.1 g activated carbon (21 wt.%) with cavity formations from 100 nm. At a ratio of 44 to 35% and 21%, a dark gray bulk product was obtained, which was added to the main feed ration while mixing in a colloid mill.

Another option for the additive can be obtained as follows.

The IKA IKA T 50 digital dispersant is loaded with amorphous silicon dioxide carrier (with mesopore sizes from 0.2 to 1.8 nm) and pharmacopoeial activated carbon, while stirring at about 1000 rpm, micellized curcumin extract was gradually added over 0.5 hours and rutin in polysorbate 80, after adding the entire amount of extract, the stirring speed was increased to 7000 rpm to increase dispersibility and uniformity of distribution of the substance. To prepare the additive, 4.4 g of amorphous silicon dioxide (44 wt.%), 3.5 g (35 wt.%) of a solution of curcumin and rutin in polysorbate 80 (curcumin 10.3 wt.%, rutin 3 wt.%, polysorbate 80 rest) and 2.1 g of activated carbon (21 wt.%) with cavity formations from 100 nm. At a ratio of 44% to 35% and 21%, a bulk product was obtained, which was added to the main feed ration while mixing in a colloid mill.

Analysis of the size of nanoparticles of active substances on the absorbent of the resulting additives was carried out using a Zetasizer Nano analyzer (Table 1).

The graph (Table 1) shows that the initial form of curcumin in polysorbate 80 in the form of micelles (yellow color) is superimposed on curcumin obtained in a dispersant (red color) on an amorphous carrier, which directly indicates the applicability of this technology for obtaining nanopowders of active substances

- 2 044377 on absorbents and thus increasing the bioavailability of the studied substrates.

Achievement of the technical result is confirmed by the conducted research.

Materials and methods of research.

A study was conducted on broiler chickens of the Foxy Chick selection from one day old to 35 days of age in a vivarium. Groups were formed using the analog method without division by sex, 35 animals each. Feeding was carried out with dry complete loose mixed feed with nutritional value according to the standards, ad libitum. The material for the study was additives of biologically active substances in crumbly form.

The first group received a basic diet of 107.56 kg of feed per group, without feed additives.

The second group received 107.38 kg of feed plus 0.25 kg of a powder additive with the composition: mesoporous silicon dioxide, curcumin, rutin, in polysorbate 80. The mixture was processed in a colloid mill and then fed to the chickens.

The third group received 107.28 kg of feed plus 0.45 kg of additive in powder form with the composition: mesoporous silicon dioxide and curcumin in polysorbate 80, activated carbon with cavity formations of 100 nm or more. The mixture was processed in a colloid mill and then fed to the chickens.

The fourth group received 107.31 kg of feed and 0.45 kg of additive in powder form, including: mesoporous silicon dioxide, activated carbon with cavity formations of 100 nm or more, curcumin and rutin in polysorbate 80.

The entire composition was processed in a dry-ground colloid mill and then fed to the chickens.

Until 3 days of age, chickens of all groups received granulated feed, then loose feed of the same nutritional value, the recipe for which is presented in Table. 2.

Physiological experiments to determine the digestibility and availability of dietary nutrients, as well as the use of calcium and phosphorus from mixed feed by broilers when nanocurcumin and nanocurcumin + rutin additives were included in the feed were carried out on cockerels (n=3) aged 30-34 days.

The digital data obtained in the experiments were processed by the method of variation statistics according to the Student criterion. The data in the tables is presented in the form M±m, where M is the arithmetic mean, m is the error of the arithmetic mean. Differences were considered significant at p<0.05. The study took into account the safety of the livestock (%) by recording mortality and establishing its causes. The live weight of broilers was determined at the age of 7-21 days and at the end of the experiment by individual weighing of the entire population and by groups in the city. Feed consumption during the study period per head and per kg of live weight gain was determined by daily recording of feed by groups. The chemical and amino acid composition of broiler meat (leg and pectoral muscles) was also determined.

Research results.

Zootechnical results of studies on the effect of nanocurcumin and nanocurcumin + rutin additives in feed for broiler chickens on a carrier are presented in Table. 3.

From the table Figure 3 shows that at 7 days of age, the live weight of chickens of the third experimental group receiving nanocurcumin was 1.59% higher than the control. By 21 days of age, broilers of this group surpassed their control counterparts in live weight by 4.6% (the difference is statistically significant at p>0.05), and by the end of growing, the average live weight of chickens in this group was 4.02% higher than the control. At the same time, the live weight of the cockerels was higher than the control by 2.0%, and that of the hens by 6.4%; the difference with the control was significant. Good digestibility of the nanocurmin + rutin additive contributed to an improvement in feed conversion in broilers of the third experimental group in comparison with the control by 7.02%, an increase in the average daily gain in live weight by 4.06%, which influenced an increase in the productivity index of broiler rearing by 41.92 points level of control. So, the live weight of chickens of the fourth group is 7; at 21 and 35 days of age it significantly exceeded the control by 2.0; 7.8 and 7.2%. At the same time, the live weight of cockerels was significantly higher than the control group by 6.2%, and that of hens by 8.4%. The use of a micellized form of nanocurcumin on a carrier had a positive effect on feed conversion and contributed to a decrease in this indicator by 11.7%. A comprehensive assessment of the productivity of broilers according to the productivity index showed that the inclusion of a micellized form of nanocurcumin on an absorbent in the feed of broilers of the fourth experimental group made it possible to ensure an increase in the productivity index of chickens in this group by 70.83 points compared to the control.

Balance sheet experience data presented in table. 5 are consistent with the obtained zootechnical results. It was established that the increase in the growth rate of chickens of the third experimental group is due to improved digestibility and availability of nutrients from mixed feed. Thus, the digestibility of protein, dry matter of feed, fat, fiber improved by - 1.55; 1.61; 1.75; 1.4% compared to the control, and the use of nitrogen, phosphorus and the availability of lysine and methionine by - 8.58; 3.3 and 1.06; 0.83%. At the same time, calcium use was at the level of the corresponding indicators in the control group.

In table Figure 4 shows the use of feed nutrients (%) by broilers aged 30-35 days, M±m, n=3.

Assessing the effect of nanocurcumin + rutin additives on a carrier at a dose of 250 g/t of feed on the digestibility and availability of feed nutrients, it should be noted that broilers of the fourth experimental group in the digestibility of protein, dry matter of feed, fat, fiber were superior to control analogues by - 1.86; 1.95; 3.55; 2.6%, and the use of nitrogen, calcium, phosphorus and the availability of lysine and methionine improved compared to the control by - 8.34; 0.95; 3.9; 1.66 and 2.73.

In table Figure 5 shows the chemical composition (%) and content of vitamins and carotenoids (µg/g) in the liver of broiler chickens at the age of 36 days.

Analysis of the chemical composition of the liver of 35-day-old broilers showed (Table 5) that the protein content in the liver of chickens of the second, third and fourth groups was higher than the control by 5.37; 8.17 and 8.05%, while there was a decrease in fat content in the liver of broilers receiving both the drug nanocurcumin + rutin and nanocurcumin by 2.76; 7.31 and 7.71%, which indirectly indicates the absence of a cytotoxic effect on liver cells from the use of applied drugs in the studied dosages.

The content of vitamin _B2 in the liver of broilers practically did not differ between groups and was in the range of 11.38-11.74 mcg/g.

In table Figure 6 shows biochemical and hematological blood parameters in 35-day-old broiler chickens (n=3).

The study of hematological and biochemical parameters in broilers showed (Table 6) that the level of total protein, leukocyte formula, and erythrocyte content in chickens of all groups practically did not differ and were within the physiological norm for the given age of chickens. Thus, the protein level was in the range of 37.7-42.9 g/l, the concentration of erythrocytes was 2.8-3.0 RBC* 10 ¹² /l. At the same time, the levels of cholesterol and uric acid in the experimental groups were statistically significantly lower than in the chickens of the control group (p <0.001) with a higher level of hemoglobin - 140.7-152.0 in the experimental groups and 124.3 in the young animals of the control group.

Given in table. 7 and 8, the results of the analysis of the chemical and amino acid composition of broiler meat (pectoral and leg muscles) showed that the inclusion of an additional source of nanocurcumin and rutin in the supplements in the diets of broilers of the second and third groups contributed to an increase in the protein content in the pectoral muscles of cockerels by 3.2 and 4 .06% due to a decrease in fat content by 3.44 and 3.35% and an increase in the amount of amino acids compared to the control by 2.53 and 2.73%. In terms of the content of essential amino acids, the pectoral muscles of chickens of the second and third groups exceeded the control by 0.9 and 0.89%.

A higher content of protein and essential amino acids was also noted in the pectoral muscles of chickens of the second and third groups - by 1.24; 1.13 and 1.78; 1.73%, with a decrease in fat content by 0.01 and 0.14%.

Additions of nanocurcumin to broilers of the fourth experimental group contributed to the improvement of meat quality. The protein content in the pectoral muscles of cockerels and hens increased compared to the control by 2.33 and 1.45%, essential amino acids - by 1.89 and 1.33%, while the fat content decreased by 1.36% in cockerels and by 0. 99% in chickens of the fourth group.

Analysis of the chemical composition of the leg muscles of broilers showed that lower fat content (by 2.16 and 3.55% in males and by 9.66 and 10.13% in hens) was noted in broilers of the second and third experimental groups that received nanocurcumin + rutin . In terms of protein content, the leg muscles of cockerels exceeded the control by 1.92 and 2.02%, and those of hens by 6.76 and 7.98%. The leg muscles of broilers of the second and third experimental groups in the content of essential amino acids exceeded the control by 0.93 and 1.25% in cockerels and by 3.18 and 3.4% in hens.

The use of a nano-sized form of curcumin had a positive effect on increasing the protein content in the leg muscles of cockerels and hens of the fourth experimental group by - 2.53 and 8.21%, due to a decrease in fat content - by 4.49% in cockerels and by 23.12% in chickens.

Analysis of chilled broiler meat (chest and leg muscles) for peroxide value showed that peroxide value % I in chickens of the second, third and fourth groups was 0.026; 0.038 and 0.049 versus 0.042% I in broiler meat of the control group.

The tasting assessment did not reveal any differences in the taste of meat and broth between the control and experimental groups.

Thus, including taking into account the data from the above studies, it has been established that the high availability of biologically active substances from the additives described above has a positive effect on the productivity of broiler chickens, providing an increase in the live weight of broilers by 4.03-7.4%, a reduction in feed costs 1 kg of live weight gain by 7.62-10.9% improves the vitamin supply of chickens.

The hepatoprotective properties of the supplements help improve the functional state of the liver by reducing fat levels by 9.3; 13.15 and 12.89%. At the same time, the levels of cholesterol and uric acid in the experimental groups were statistically significantly lower than in the chickens of the control group (p <0.001) with a higher level of hemoglobin - 140.7-152.0 in the experimental groups and 124.3 in the young animals of the control group. The positive effect of additives on the intensity of protein, carbohydrate and lipid metabolism helps to increase the protein content in the chest and leg muscles of broilers

- 4 044377 due to the reduction in fat content, which can contribute to the possibility of increasing the shelf life of meat, by reducing the formation of free radicals during meat storage.

Table 1

4035

1.0

AND

10.0

100.0

1000.0

6500.0

Size Distribution Diameter/nm

table 2

Composition and nutritional value of feed in the control group %

Component 3-21 days 22-34 days Wheat 56.10 56.23 Soybean meal 20.0 33.0 Limestone 4.0 2.6 Sodium chloride 0.12 0.18 Vegetable oil 3/2 4.1 Lysine 0.11 0.15 Methionine 0.2 0.32 Threonine 0.17 0.24 Soybeans 1.1 1.5 Bran 2.4 3.0 Choline chloride 0.18 2.2 Sodium sulfate 1.4 1.5

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Table 3

Main zootechnical results of the experiment on broilers

Index Group 1 to) 2 3 4 Livestock safety, % 100 100 100 100 Live weight, g aged, days: daily allowance 42.0±0.34 42.3±0.41 42.2±0.39 42.0±0.34 7 169.3± 166.23± 172.0±2.1 175.80± 2.24 2.44 +1.59% 2.02 +2.0% 21 839.11± 859.94± 877.71± 904.31± 12.17 13.53 14.51 ¹ 14.45 ³ +2.5% +4.6% +7.8% 35 (average) 1993.3 2022.4 +1.46% 2073.48 +4.02% 2136.74 +7.2% Group Index 1 to) 2 3 4 Livestock safety, % 100 100 100 100 Live weight, g at age, days: daily allowance 42.0±0.34 42.3±0.41 42.2±0.39 42.0±0.34 7 169.3± 166.23+ 172.0+2.1 175.80± 2.24 2.44 +1.59% 2.02 +2.0% 21 839.11± 859.94± 877.71± 904.31± 12.17 13.53 14.51 ¹ 14.45 ³ +2.5% +4.6% +7.8% 35 (average) 1993.3 2022.4 +1.46% 2073.48 +4.02% 2136.74 +7.2%

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Table 4

Use of feed nutrients (%) by broilers aged 30-35 days, M±w, n=3

Index Group 1 to) 2 3 4 Digestibility: protein 93.72+ 0.4 92.9±0.4 95.27±0.47 95.58± 0.47 feed dry matter 74.63± 0.3 76.26±0.3 7b.24± 0.40 76.58± 0.40 fat 84.90± 0.3 84.80+0.4 86.65± 0.44 88.40± 0.44 fiber 18.3+ 0.8 18.2+0.8 19.7±0.7 9 20.90±0 0.79 Usage: nitrogen 60.66± 0.3 67.50±0.3 69.25± 0.27 69.0± 0.27 calcium phosphorus 48.9±0.2 32.6±0.1 48.75+0.2 32.7±0.1 48.34+ 0.20 35.9±0.12 49.85± 0.20 36.50± 0.12 Availability: Methionine Lysine 88.0±0.5 84.21± 0.4 89.71±0.4 86.40±0.5 89.06± 0.44 85.04± 0.39 89.66± 0.45 8b.94± 0.40

Table 5

Chemical composition (%) and content of vitamins and carotenoids (µg/g) in the liver of broiler chickens aged 36 days

Index Group 1 to) 2 3 4 Moisture 65.61 68.84 70.89 67.79 Crude protein 58.81 64.18 66.98 66.86 Crude fat 23.38 20.62 16.07 15.67 Raw ash 4.05 4.45 4.64 4.62 Vitamin A 135.23 158.40 170.39 179.15 Vitamin E 14.71 15.19 17.53 17.59 Vitamin Bg 11.74 11.54 11.38 11.70 Carotenoids 0.49 0.43 0.69 0.77

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Table 6

Biochemical and hematological blood parameters in 35-day-old broiler chickens, (n=3)

Index Group 1 to) 2 3 4 Total protein, g/l 38.90±0.78 37.7±0.57 42.9±3.06 40.0±1.50 Trypsin, units/l 497.9±59.3 378.4± 26.28 58b.1±35.69 519.9±35.69 Glucose, mmol/l 14.8±0.26 13.9±0.05 12.6±1.42 12.8±0.47 Cholesterol, mmol/l 2.15±0.07 1.76± 0.029*** 1.89±0.19*** 1.83±0.10*** Uric acid, µmol/l 359.1±18.82 283.3+ 7.92** 256.6± 18.70*** 222.3± 17.21*** ALT, units/l 41.4±6.43 49.3±5.77 36.1±1.35 30.1±0.63 AST, units/l 351.7±1.34 348.9±17.13 392.0±11.19 401.0±52.57 Alkaline phosphatase, units/l 10219.8+ 2246 14722± 2199 10932±1773 10932±1773 Leukocytes WBC*10 ⁹ ^ 35.8±2.36 31.3+1.60 38.2±2.99 32.8±0.70 Pseudoeosino phyla, % 36.5±1.02 43.5+6.06 55.5±1.76 45.7±4.19 Lymphocytes, % 56.7±1.90 51.7±4.74 31.6±5.10 45.6±5.43 Monocytes, % 0.3±0.09 0.4±0.07 3.6±2.93 0.40±0.10 Eosinophils, % 6.0±1.03 4.2±1.42 9.0±2.78 8.0±1.10 Basophils, % 0.4±0.15 0.3+0.09 0.2±0.09 0.3+0.09 Erythrocytes, VSV*10 ¹² /l 2.9±0.12 2.8±0.10 3.0±0.09 3.0±0.01 Hemoglobin, g/l 124.3±6.77 140.7±4.81 152.0±3.51 146.3±0.88 Hematocrit, % 37.0±1.60 35.5±1.29 38.3±0.66 37.3±0.30

**p<0.01;***p<0.001

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Table 7

Chemical composition and content of amino acids in the homogenate of broiler breast muscles, (% of air dry matter)_______

Index cockerels chickens Group 1 to) 2 3 4 1 to) 2 3 4 Moisture 69.50 69.20 71.40 70.35 73.61 71.17 66.86 69.25 Crude protein 85.60 88.80 89.66 87.93 88.23 89.47 89.36 89.68 Crude fat 6.61 3.17 3.26 5.25 3.60 3.59 3.45 2.61 Raw ash 4.42 4.66 4.47 4.43 4.40 4.53 4.51 4.65 Lysine 7.51 7.74 7.76 7.62 7.45 7.65 7.67 7.60 Valin 4.39 4.49 4.39 4.49 4.26 4.45 4.48 4.30 Methionine 2.24 2.35 2.39 2.34 2.33 2.33 2.34 2.34 Isoleucine 4.09 4.20 4.21 4.20 4.07 4.18 4.12 4.08 Leucine 6.61 6.76 6.78 6.65 6.57 6.74 6.69 6.70 Threonine 3.65 3.81 3.82 3.79 3.73 3.77 3.78 3.74 Phenylalanine 3.40 3.44 3.43 3.42 3.34 4.41 4.40 4.32 Σ essential amino acids 31.89 32.79 32.78 32.51 31.75 33.53 33.48 33.08 Alanin 4.88 5.02 4.92 4.91 4.85 4.98 4.99 4.92 Cystine 0.97 0.99 0.98 0.98 0.98 0.98 1.0 1D Histidine 3.09 3.07 3.08 3.10 3.12 2.98 3.14 3.15 Arginine 5.51 5.53 5.55 5.54 5.41 5.44 5.46 5.42 Aspartic acid 7.70 7.96 7.93 7.95 7.70 7.73 7.73 7.72 Tyrosine 2.91 3.01 2.98 2.95 2.94 2.91 2.95 2.95 Serin 3.05 3.29 3.28 3.20 3.20 3.21 3.20 3.21 Glutamic acid 11.83 12.47 12.83 12.95 12.05 12.26 12.27 12.28 Proline 2.95 3.01 3.04 3.03 2.93 3.01 3.05 3.01 Glycine 3.55 3.72 3.69 3.72 3.55 3.57 3.58 3.65 Σ substitutions, amine. 46.44 48.07 48.28 48.33 46.73 47.07 47.37 47.41 Sum of amino acids 78.33 80.86 81.06 80.84 78.48 80.60 80.85 80.49

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Table 8 Chemical composition and amino acid content in the leg muscle homogenate of __________broilers, (% of air dry matter)_________

Index cockerels chickens Group 1 to) 2 3 4 1 TO) 2 3 4 Moisture 67.47 67.42 65.16 62.59 64.01 70.08 67.12 67.24 Crude protein 66.99 68.91 69.01 69.52 60.83 67.59 68.81 69.04 Crude fat 27.66 25.50 24.11 23.17 34.21 24.55 24.08 23.12 Raw ash 3.71 3.84 3.44 3.23 3.28 3.90 3.57 3.56 Lysine 5.19 5.49 5.65 5.91 4.87 5.57 5.18 5.66 Valin 3.19 3.31 3.34 3.39 2.98 3.33 3.36 3.53 Methionine 1.84 1.87 1.89 1.85 1.76 1.96 1.99 1.98 Isoleucine 3.05 3.14 3.32 3.78 2.81 3.22 3.22 3.33 Leucine 4.90 5.07 5.05 5.08 4.45 5.16 5.94 5.94 Threonine 2.76 2.87 2.84 2.69 2.48 2.90 2.75 2.83 Phenylalanine 2.53 2.64 2.62 2.42 2.29 2.68 2.60 2.65 Σ 23.46 24.39 24.71 25.12 21.64 24.82 25.04 25.92 Alanin 3.78 4.04 4.08 4.53 3.57 4.04 4.58 4.66 Cystine 0.73 0.74 0.75 0.67 0.66 0.78 0.79 0.78 Histidine 2.23 2.24 2.26 2.14 2.05 2.25 2.25 2.13 Arginine 3.75 4.04 3.78 3.67 3.53 4.03 4.15 3.59 Aspartic acid 5.55 5.82 5.64 5.21 5.14 5.89 5.94 5.84 Tyrosine 2.12 2.22 2.25 2.04 1.94 2.27 2.29 2.29 Serin 2.38 2.49 2.42 2.41 2.12 2.46 2.16 2.30 Glutamic acid 9.16 9.69 9.41 9.73 8.11 9.61 9.78 9.67 Proline 2.62 2.76 2.74 2.67 2.42 2.67 2.74 2.81 Glycine 3.12 3.41 3.86 3.81 2.87 3.19 3.31 3.95 Σ 32.32 37.45 37.22 36.88 32.41 37.19 37.99 38.02 Sum of amino acids 55.78 61.84 61.90 62.0 54.05 62.01 63.03 63.94

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Claims (11)

CLAIM 1. Feed additive to feed for broiler chickens, including amorphous mesoporous silicon dioxide: 64 wt.%, a solution of curcumin and rutin in polysorbate 80: 36 wt.%. 2. Feed additive according to claim 1, characterized in that the size of the mesopores of amorphous silicon dioxide is from 0.2 to 1.8 nm, and the solution of curcumin and rutin in polysorbate 80 has the following composition: curcumin 10.3 wt.%, rutin 3 wt.%, polysorbate 80 the rest. 3. Feed additive to feed for broiler chickens, including amorphous mesoporous silicon dioxide: 44 wt.%, curcumin solution in polysorbate 80: 35 wt.%, activated carbon with cavity formations of 100 nm or more: 21 wt.%. 4. Feed additive according to claim 1, characterized in that the size of the mesopores of amorphous silicon dioxide is from 0.2 to 1.8 nm, and the solution of curcumin in polysorbate 80 has the following composition: curcumin 10.3 wt.%, polysorbate 80 the rest. 5. Feed additive to feed for broiler chickens, including amorphous mesoporous silicon dioxide: 44 wt.%, a solution of curcumin and rutin in polysorbate 80: 35 wt.%, activated carbon with cavity formations from 100 nm: 21 wt.%. 6. Feed additive according to claim 5, characterized in that the size of the mesopores of amorphous silicon dioxide is from 0.2 to 1.8 nm, and the solution of curcumin and rutin in polysorbate 80 has the following composition: curcumin 10.3 wt.%, rutin 3 wt.%, polysorbate 80 the rest. 7. A method for producing a feed additive to feed for broiler chickens, including loading amorphous silicon dioxide or amorphous mesoporous silicon dioxide mixed with activated carbon into a dispersant, stirring at a speed of about 1000 to about 2000 rpm for about 0.5 h with the gradual addition of micellized curcumin extract in polysorbate 80 or micellized curcumin and rutin extract in polysorbate 80, increasing the mixing speed to 7000-9000 rpm until a homogeneous product is obtained. 8. The method for producing a feed additive according to claim 7, characterized in that the size of the mesopores of amorphous silicon dioxide is from 0.2 to 1.8 nm. 9. The method for producing a feed additive according to claim 7, characterized in that the active carbon includes cavity formations with a diameter of 100 nm or more. 10. The method for producing a feed additive according to claim 7, characterized in that the solution of curcumin and rutin in polysorbate 80 has the following composition: curcumin 10.3 wt.%, rutin 3 wt.%, polysorbate 80 the rest. 11. The method for producing a feed additive according to claim 7, characterized in that the solution of curcumin in polysorbate 80 has the following composition: curcumin 10.3 wt.%, polysorbate 80 the rest. Eurasian Patent Organization, EAPO Russia, 109012, Moscow, Maly Cherkassky lane, 2 -