EA027863B1 - Disinfecting agent - Google Patents

Abstract

The invention is related to agriculture, in particular, to veterinary sanitary, and can be used for disinfection of items subject to veterinary supervision. The technical result provided by the invention is addition of agents that improve bactericide properties of the preparation and soften water hardness. The disinfecting agent comprising glutaric aldehyde and water includes additionally benzalconium chloride and trilon B with the following proportion of the components, wt.%: glutaric aldehyde - 0.5-2.0, benzalconium chloride - 0.5-2.0, trilon B - 0.05-0.2, water - the balance.

Description

The invention relates to agriculture, in particular to veterinary sanitation, and can be used to disinfect objects of veterinary surveillance.

A disinfectant is known, including glutaraldehyde and water [Disinfection of livestock buildings and veterinary sanitation in transport; - M: VNIIVS, 1983, p. 37].

The disadvantage of this disinfectant is its high concentration of aldehyde.

The objective of the invention is to increase the antimicrobial properties of the disinfectant composition due to the synergistic effect that increases with increasing exposure time and softening the hardness of the water.

The technical result provided by the invention is expressed by the addition of agents that enhance the bactericidal properties of the drug and soften the hardness of the water.

Compositional disinfectant, including glutaraldehyde and water, additionally contains Catcell and Trilon B in the following ratio, wt.%:

glutaraldehyde 0.5-2.0 catellus 0.5-2.0 trilon B 0.05-0.2 water rest

Glutaraldehyde is a complex organic substance with sterilizing and disinfecting properties. Glutaraldehyde belongs to the group of aldehydes (from lat. A1co1o1 4eNu4godeia1ish - alcohol lacking hydrogen), chemical formula: C ₅ H ₈ O ₂ . Glutaraldehyde is a clear, colorless liquid with a pungent fruity odor containing 50-51% of the active substance.

Catsell is a solution of alkyldimethylbenzylammonium chloride containing 49-51% of the basic substance. Catsell is a light yellow viscous liquid with a faint, specific smell of detergent. Mixes with water in any ratio, slow-burning, not explosive. Catsell is a bactericidal agent that is effective against a wide range of gram-positive bacteria, and at high concentrations also against some gram-negative bacteria.

Trilon B (versen) is a white crystalline powder, odorless, salty taste, well soluble in water. The solutions are very stable (do not decompose with prolonged boiling), are part of the production of household chemicals and synthetic detergents, as a stabilizer.

Example 1. In an apparatus for stirring, water is poured in an amount of 878 ml, Trilon 0.2 g is stirred until complete dissolution. Then, 60% Catsell (50%), 60 ml glutaraldehyde (60 ml) are added and mixed again. 1000 ml of disinfectant are obtained.

The bactericidal activity of the composition was determined in accordance with the guidelines on the procedure for testing new disinfectants for veterinary practice (1987).

To do this, washings of one-day agar cultures of E.coN and δΐ were applied to sterile test objects (wood, brick, tile). igeeik mixed with sterile manure at the rate of 0.2 g per 100 cm ^{2 of the} test object, so that 1 million ^{2 of the} area of the test object accounted for 20 million microbial bodies. After that, test objects in sterile conditions were left for 18-20 hours to dry. Next, a composition from 0.05 to 2.0% concentration was applied to test objects using a spray gun, at the rate of 5 ml per 100 cm ² . Then, after 1, 3, and 6 hours, samples were taken from the surfaces of the test objects with a sterile cotton swab and examined for the presence of test microbes.

As a control, contaminated test objects were treated with sterile water.

Example 2. A disinfectant is used in the following ratio of components: glutaraldehyde 0.5% catelli 0.5% trilon B 0.05% water the rest

As a result of the studies, it was found that preventive disinfection of the surfaces of veterinary surveillance objects can be carried out with wet disinfection at a flow rate of up to 0.5 l / m ² and exposure up to 1 hour. When processing elements from porous materials (wood, concrete, brick, etc.) d.) the consumption rate is increased to 1.0 l / m ² with an exposure of 3 hours or more.

Example 3. The disinfectant is used in the same way as in example 2 in the following ratio of components:

glutaraldehyde 1.0% catsell 1.0% trilon B 0.1% water else

As a result of the studies, it was found that the current and final disinfection

- 1 027863 surfaces of objects of veterinary supervision for brucellosis can be carried out with wet disinfection at a flow rate of up to 1.0 l / m ² and exposure up to 1 hour.

Example 4

A disinfectant is also used as in example 2 in the following ratio of components:

glutaraldehyde 2.0% catzell 2.0% trilon B 0.2% water else

Forced (current and final disinfection) surfaces of objects of veterinary surveillance for tuberculosis can be carried out with wet disinfection at a flow rate of up to 1.0 l / m ² and exposure up to 1 hour.

Example 5

The developed disinfectant composition was tested under the conditions of production in the farm Mereka Ushtobinsky s / o Bukharzhyrau district of Karaganda region.

In the prepared room, before disinfection in various places, test objects contaminated with the test culture M. pBe1 were placed, after which disinfection was carried out. The quality of disinfection was determined in accordance with the guidelines on the procedure for testing new disinfectants for veterinary practice (1987). For this, sterile test objects (wood, brick, tile) were washed with M. pYe1 culture mixed with sterile manure at the rate of 0.2 g per 100 cm ^{2 of} test object, so that about 1 cm ^{2 of} the test object area 20 million microbial bodies. After that, the test objects in sterile conditions were left for 18-20 hours to dry. Then, wet disinfection was carried out by small-drop irrigation of surfaces with a solution of the Glukat preparation prepared in tap water (18-20 ° C). In the experiment, solutions of the drug were tested in 0.1, 0.5, 1.0, 1.5 and 2.0% concentrations at a rate of 1.0 l / m ² and exposure for 3 and 6 hours. After disinfection was completed, the windows were closed and the doors of the room were left for 6 hours. Then, after 3 and 6 hours, samples were taken from the surfaces of the test objects with a sterile cotton swab and examined for the presence of test microbes. As a control, contaminated test objects were treated with sterile water.

As a result of production experience, it was found that M. pYe1 showed resistance to a drug concentration of 1%.

Thus, the forced (current and final disinfection) of surfaces of veterinary surveillance facilities in farms that are unsuccessful for tuberculosis should be carried out with a solution of the disinfectant composition with wet disinfection of 2% concentration at a flow rate of 1.0 l / m ² and an exposure of 3 hours.

Example 6

According to the results of the studies, it was found that a 2.0% solution of the disinfectant composition has a bactericidal, bacteriostatic effect on mycobacteria and allows us to recommend the proposed disinfectant for disinfection of cattle-breeding facilities unsuccessful for tuberculosis and other objects of veterinary surveillance.

The use of the proposed disinfecting composition for disinfecting veterinary surveillance facilities will allow for preventive, forced and final disinfection by irrigation, which is industrially applicable in livestock farms.

Claims (1)

A disinfectant, including glutaraldehyde and water, characterized in that it additionally contains Catcell and Trilon B in the following ratio, wt.%: Glutaraldehyde 0.5-2.0; katsell 0.5-2.0; Trilon B 0.05-0.2; water is the rest.