EA014063B1 - Detergent composition for disinfection of cutaneous covering and method of preparing thereof - Google Patents

Abstract

The invention relates to production of detergent agents, particular to detergent compositions for disinfection of skin and can be used as hygienic care for human's skin in both everyday life and in professional use in medical institutions and in household facilities. According to the invention there proposed a rheologically modified detergent composition for disinfection of skin comprising surfactants as sodium lauretsulfat and/or betaines, a rheological modifier selected from the group comprising methylcellulose, methylethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, one or several antimicrobial agents selected from the group comprising 2-bromine-2-nitroproponediol-1,3-methyl, methylparaben, propylparaben, oil of tea tree, N,N-bis(3-aminopropyl)dodezylamin, and also citric acid, target additives and water if necessary with the following component ratio, wt %:As target additives the detergent composition comprises together or in any combination a chelating compoundin the form of sodium salts of ethylenediaminttetraacetic acid- 0.01-1.0 wt %, polyethelene glycol or glycerol or propyleneglycol- 0.1-10.0 wt %, diethanolamides of fatty acids – 0.5-10.0 wt %, sodium chloride – 0.01-5.5 – wt %, aminoksids – 0.1-8.0 wt %, allantoin – 0.05-1.0 wt %, sodium acetate -0.01-10.0 wt %, perfume odorant – 0.01-0.5 wt %. A method of preparing the detergent composition comprises pre-hydration and dissolving a rheological modifier in water at temperature not exceeding 50°C and subsequent mixing thereof at temperature not exceeding 40°C with other components added separately or in a combination and agitating the mixure to obtain a homogenous substance.

Description

The invention relates to the production of detergents, namely, detergent compositions for disinfection of the skin, and can be used both for hygienic care of human skin in everyday life, and for professional use in medical and public services institutions. The invention provides a rheologically modified detergent composition for disinfection of the skin, containing surfactants in the form of sodium and / or betaine laureth sulfate, a rheological modifier selected from the group consisting of methyl cellulose, methyl ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, one or more selected antimicrobial agents a group including 2-bromo-2-nitropropanediol-1,3, methylparaben, propylparaben, tea tree oil, X, Y-bis- (3-aminopropyl) dodecylamine, and also citric acid, target additives, if necessary, and water, in the following ratio of components, wt.%:

014063 Β1

Surfactants 0.1-26.0 Rheological modifier 0.01-5.0 Antimicrobial agents 0.001-2.0 Citric Acid 0.01 -1.0 Targeted Supplements 0.01-27.0 Purified or Drinking Water up to 100

014063 Β1

As target additives, the washing composition contains, together or in any combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerin, or propylene glycol - 0.1-10.0 wt. %, fatty acid diethanolamides - 0.5-10.0 wt.%, sodium chloride - 0.01-5.5 wt.%, amine oxides 0.1-8.0 wt.%, allantoin - 0.05-1 , 0 wt.%, Sodium acetate - 0.01-10.0 wt.%, Perfume fragrance - 0.01-0.5 wt.%. The method of preparing the washing composition consists in preliminary hydration and dissolution of the rheological modifier in water at a temperature not exceeding 50 ° C, followed by its mixing at a temperature not exceeding 40 ° C with other components, administered separately or in combination, and mixing the mixture until a homogeneous mass is obtained .

The invention relates to the production of detergents, namely, detergent compositions for disinfection of the skin, and can be used both for hygienic care of human skin in everyday life, and for professional use in medical and public services institutions. The invention also relates to a method for preparing a detergent composition.

Hand washing is the most effective way to prevent the spread of bacteria and viruses, which can lead to infectious diseases and food poisoning. Hand treatment (washing and disinfection) is one of the most effective measures for the prevention of nosocomial infections, which provides protection for both medical personnel and patients.

Hand hygiene is a general term used to refer to procedures such as regular hand washing and hygienic hand disinfection. Normal hand washing - wash your hands with water and ordinary soap or detergent (without antiseptics). Hygienic hand washing - wash your hands with soap and water or another detergent that contains an antiseptic. Hygienic hand washing refers to one of the methods of hygienic hand disinfection that allows you to kill or stop the growth of microorganisms, this process is called chemical removal of microorganisms (Ιπίοοΐίοη Οοηίτοί aib Ηοφίΐαί Ер1бетю1оду, 1997, 722-727). In modern literature, hand hygiene of nurses is considered as one of the most important measures of infection control, allowing you to break the chain of development of nosocomial infections (Ιηίοοίίοη Οοηίτοί 23 (2): 87-94).

Modern detergents for hygienic purposes are, as a rule, aqueous solutions, gels, emulsions based on surface-active substances (surfactants). They may additionally include antimicrobial agents, perfumes, dyes, special additives that improve the consumer properties of detergents.

The choice of a hygienic detergent depends on many factors, including, first of all, a clear idea of the purpose of the product and its scope. For disinfection of the skin, the choice of detergent is determined by the species composition of microflora on the surface of the skin, the antimicrobial activity of ίη νίίτο and the antiviral activity of the agent, the intensity, duration and frequency of professional actions, the condition of the skin after frequent use of the detergent, as well as its color, smell, texture, foaming ability and, no less important, cost.

It is well known that the main action of all detergents is the cleaning of contaminants. Despite the very wide variety of skin-care detergents on the cosmetic market, it’s not easy to choose a simple detergent for regular washing of hands by medical staff, since, first of all, the specific condition of the skin of the hands of medical workers, especially those working in hospitals. Cheap detergents are aggressive in relation to the skin of the hands, since they are created using cheap hard surfactants and simple cooking technology. The use of better basic detergents with mild detergent properties or the introduction of additional special structuring components that improve the consumer properties of the final product lead to a significant increase in the cost of hygienic detergents.

Surfactants are the key components of detergent compositions that determine the detergent properties of the product, the level and structure of the foam, and the dermatological properties of the formulations. Surfactants with excellent detergency and foaming, at the same time, are the most dermatologically harsh and cause skin irritation. Soft surfactants have reduced foaming, which affects the quality of the final product, and, as a consequence, on consumer properties. The introduction of biocidal additives into the detergent composition creates additional problems for manufacturers, since antibacterial components affect both foaming and the dermatological characteristics of the product as a whole, as well as its antimicrobial properties. There are strict requirements for this category of products, which are limited by the properties of the corresponding surfactants and biocides. To achieve a strong antibacterial effect with a mild effect on the skin of the detergent composition is very difficult. Therefore, the creation of a mild detergent composition for ordinary washing and hygienic washing of the skin is a very urgent and promising task.

Known antimicrobial detergent for disinfection of the skin (\ UO 97/48377), containing as an antibacterial additive triclocarban (TCC) - 0.00705-0.15 wt.%, And as washing components - soft surfactants in an amount of 5.0 -15.0 wt.% And additionally containing lipophilic thickeners in the form of derivatives of vinyl and acrylic acid, preservatives, colorants and perfumes.

Liquid antibacterial compositions are known (UO 2007/092090), containing triclocarban as an antibacterial additive in an amount of 0.01-10.0 wt.%, As detergent components one or more surfactants - 0.1-40.0 wt.% , one or more cosolvents - 10-60 wt.%, the rest, up to 100 wt.%, are dyes, preservatives, perfumes, pH regulators and chelating agents. The most serious drawback of the above compositions is the use of triclocarbane as the main biocide. Triclocarban is widely used in household products.

- 1 014063 howl chemistry and personal hygiene, including in liquid and solid soaps, cleansing lotions, wet wipes. For more than 45 years, products containing triclocarban have been widely sold in the United States and Europe. Recent studies by American scholars B111 BaNsu Thu1osagiai siyaysk Vysgoys asoyoi: But the truth is that there is only one lesson to say that all of this is true. 149, No. 3, 2007, revealed two negative properties of this compound. First, in laboratory conditions in human cells, triclocarban increases the expression of genes that are normally regulated by testerone. And secondly, the addition of triclocarban to the food of male rats led to an abnormal increase in testosterone-sensitive organs, such as the prostate gland. The mechanism of action of triclocarbane differs from the mechanism of action of all known hormone-disrupting compounds, which simply usually block or reduce the effects of hormones. Despite not quite adequate experiments to study the effect of substances for external use on the body, these studies can (at least partially) explain the already recorded increase in the frequency of reproductive problems.

It is known (\ νϋ 98/02139) a liquid antiseptic soap containing active components in an amount of 10.1-32.0 wt.%, 10.0-30.0 wt.% Surfactants, 0.05-2.0 wt.% keratolytic agent in the form of tartaric acid, 0.05-0.5 wt.% biocidal additives in the form of triclosan, 0.001-0.1 wt.% additional antiseptic in the form of thymol, as well as additional components, which include preservatives, thickeners in the form polyvinyl acetate, or polyvinyl alcohol, or acrylic acid, moisturizers and substances that give the soap a cooling effect. There are no data on the antibacterial activity of the indicated antiseptic soap. The disadvantage of this composition is the use of the well-known triclosan as an active component. In the work Oo1ba1st Ε.ν. Z.aigsik 81gash agds1u Vs8181ai1 Tps1o8ai 1 °, 44 ^'s 1SAAS' №a8Yid1oi 2004, data are of very high level of resistance to triclosan 81arNu1osossi5 aigsik among strains with intermediate resistance to glycopeptides.

The closest in essence to the proposed invention (prototype) is the liquid disinfecting soap described in VI 2221548 containing the active components in the form of benzyl alcohol - 0.001-0.0025 wt.%, Allantoin - 0.3-0.8 wt.% Or chlorhexidine bigluconate - 0.3-0.8 wt.%, methylchloroisothiazolinone - 0.00005-0.0005 wt.%, methylisothiazolinone - 0.0001-0.0018 wt.%, and sodium laureth sulfate - 4.0-7, 08 wt.%, Disodium laureth-3 sulfosuccinate - 0.5-2.08 wt.%, Cocoamphoacetate sodium - 0.5-3.08 wt.%, Coconut fatty acid diethanolamide - 2.0-4.0 wt. % ethoxylated monoethanol rapeseed oil fatty acid medium - 1.0-3.0 wt.%, ethoxylated glycerin - 0.5-2.0.8 wt.%, dye - 0.005-0.05 wt.%, perfumes - 0.04- 0.2 wt.%, Sodium chloride 0.1-0.8 wt.%, Citric acid - 0.05-0.3, wt.% And water. Data on dermatological parameters and the disinfecting effectiveness of the described composition are not available.

The presence in the specified preparation of a set of preservative additives in the permitted concentration range without any necessary studies on the effectiveness of the entire system as a whole is ίη ν 11th, so 1 and νί \ Ό according to the current regulatory documents does not allow to evaluate the disinfecting ability of liquid soap. The main and very serious drawback of the composition according to the invention of VI 2221548 is resistance to mycobacteria (in particular, Prgo1c8), yeast and dermatophytes, Pseudomonas aeruginosa and viruses of the biocidal component chlorhexidine bicluconate used. Another significant disadvantage of the prototype is the unproven alternative replacement of the chlorochexidine bicluconate biocide with the well-known therapeutic additive - allantoin, attributing the latter an adequate antiseptic activity. The fact of the presence of anionic surfactant, sodium laureth sulfate, widely used in detergents, as a part of liquid soap, according to the application of VI 2221548, casts doubt on the presence of a high disinfecting activity of soap. In the work 1 and £, the failures of Soi! Go1 Tobau: Naib Nuduis, S'oh'sg 81ogu, Esiagiu 2006; SYOLSKh1bsh DysoshIs: Torya1: Moyodgary: SNSPiDgu aib 81a11iu, νΕΒΜΌ, experimental data on the inactivation of the antimicrobial activity of chlorhexidine bicluconate in the presence of anionic surfactants are presented, the latter are used to neutralize it. Another disadvantage of the prototype is that the use of a conventional viscosity regulator - sodium chloride - does not allow to obtain a high viscosity detergent composition from soft surfactants that are added to the formulation to mitigate skin irritation, and the rheological characteristics of the prototype wish to remain the best.

The present invention is to provide a detergent composition for disinfection of the skin, free from the above disadvantages, and a method for its preparation.

According to the invention, the problem is solved by the claimed rheologically modified detergent composition for disinfection of skin containing surfactants in the form of sodium laureth sulfate and / or betaines, a rheological modifier selected from the group consisting of methyl cellulose, methyl ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, one hydroxypropyl cellulose, one hydroxypropyl cellulose selected from the group consisting of 2-bromo-2nitropropanediol-1,3, methylparaben, propylparaben, tea tree oil, Ν, Ν-6πο- (3am inopropyl) dodecylamine, as well as citric acid, target additives, if necessary, and water, in the following ratio of components, wt.%:

- 2 014063

Surfactants 0.1-26.0 Rheological modifier 0.01-5.0 Antimicrobial agents 0.001-2.0 Citric Acid 0.01 - 1.0 Targeted Supplements 0.01-27.0 Purified or Drinking Water up to 100

As target additives, the inventive washing composition contains, together or in any combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerin, or propylene glycol - 0.1-10.0 wt. .%, diethanolamides of fatty acids - 0.5-10.0 wt.%, sodium chloride - 0.01-5.5 wt.%, amine oxides - 0.1-8.0 wt.%, allantoin - 0.05 -1.0 wt.%, Sodium acetate - 0.01-10.0 wt.%, Perfume fragrance - 0.01-0.5 wt.%.

In one embodiment of the invention, the detergent composition is a liquid soap that contains sodium and betaine laureth sulfate as surfactants and, in combination or separately, contains 2-bromo-2-nitropropanediol-1,3, methylparaben, as antimicrobial agents propyl paraben in the following ratio, wt.%:

Sodium Laureth Sulfate 1-16,0 Betaines 0.1 -10.0 Rheological modifier 0.01-5.0 Antimicrobial agent 0.001-1.0 Citric Acid ο, οι - ι, ο Targeted Supplements 0.01-27.0 Purified or Drinking Water up to 100

Moreover, as target additives, this composition contains, together or in combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerin, or propylene glycol - 0.1-10.0 wt. %, diethanolamides of fatty acids - 0.5-10.0 wt.%, sodium chloride - 0.01-5.5 wt.%, perfume fragrance - 0.01-0.5 wt.%.

In another embodiment of the invention, the washing composition is a liquid soap and contains sodium laureth sulfate as a surfactant, and as an antimicrobial agent it contains, in combination or separately, 2-bromo-2-nitropropanediol-1,3, methylparaben, propylparaben, and also additionally contains glycol monostearates or glycol distearates or mixtures thereof, providing a pearlescent color of the product, in the following ratio, wt.%:

Sodium Laureth Sulfate 1-16,0 Glycol monostearates or glycol distearates or mixtures thereof 0.2-5.0 Rheological modifier 0.01-5.0 Antimicrobial agent 0.001-1.0 Citric Acid 0.01 - 1.0 Targeted Supplements 0.01-27.0 Purified or Drinking Water up to 100

Moreover, as target additives, this composition contains, together or in combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerin, or propylene glycol - 0.1-10.0 wt. %, diethanolamides of fatty acids - 0.5-10.0 wt.%, sodium chloride - 0.01-5.5 wt.%, perfume fragrance - 0.01-0.5 wt.%.

In yet another embodiment, the detergent composition is a liquid soap and contains sodium and betaine laureth sulfate as surfactants, contains tea tree oil, methyl paraben and / or propyl paraben in the following ratio, wt.%: As antimicrobial agents

Sodium Laureth Sulfate 1-16,0 Betaines 0.1-10.0 Rheological modifier 0.01-5.0 Tea tree oil 0.05-1.5 Methylparaben and / or propylparaben 0.005-1.0 Citric Acid 0.01 -1.0 Targeted Supplements 0.01-22.0 Purified or Drinking Water up to 100

- 3 014063

Moreover, the composition as target additives contains, together or in combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.011.0 wt.%, Polyethylene glycol, or glycerin, or propylene glycol - 0.1-10.0 wt.%, Fatty diethanolamides acids - 0.5-10.0 wt.%, allantoin - 0.05-1.0 wt.%.

In a further embodiment, the detergent composition is a means for hygienic washing of skin and contains betaines as an antimicrobial agent Ν.Ν-bis-СЗ-aminopropyl) dodecylamine in the following ratio, wt.%:

Betaines 0.1 -10.0 Ν, Ν-bis- (3-aminopropyl) dodecylamine 0.01 - 2.0 Rheological modifier 0.01 - 5.0 Citric Acid 0.01-1.0 Targeted Supplements 0.01-20.5 Drinking water up to 100

As target additives, the product for hygienic washing of the skin contains together or in combination a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Amine oxides - 0.1-8.0 wt.%, Allantoin - 0.05-1.0 wt.%, Sodium acetate - 0.01-10.0 wt.% And perfume fragrance - 0.01-0.5 wt.%.

Another object of the invention is a method for preparing a detergent composition, which consists in preliminary hydration and dissolution of a rheological modifier in water at a temperature not exceeding 50 ° C, followed by its mixing at a temperature not exceeding 40 ° C with the remaining components, introduced in any order separately or in combination, and stirring the mixture until a homogeneous mass.

The washing composition of the present invention has an excellent cleaning effect, a safe and mild effect on the skin with frequent use, a high level of antimicrobial effectiveness and meets all regulatory requirements for modern liquid detergents for disinfection of the skin.

The indicated properties of the claimed composition are determined mainly by the introduction of a rheological modifier into the detergent composition capable of providing the necessary rheological characteristics of the detergent composition while preserving the antimicrobial activity of the product.

There are several ways to adjust the viscosity of detergents, one of which is the introduction of thickeners in the formulations. Thickeners are an integral component of all formulations of detergent compositions, and sometimes they not only regulate the viscosity, but also affect the rheological characteristics of the stability and fluidity of the product, which ultimately determines its quality. Today, more than 500 different thickeners are known, but a universal thickener does not exist. Their main classification is based on solubility in water or organic solvents.

For the detergent compositions of the present invention, a natural water-soluble thickener is selected — a nonionic polymer from the group of cellulose ethers, including methyl cellulose, methyl ethyl cellulose, hydroxyethyl cellulose or hydroxypropyl cellulose. The detergent composition of the present invention has improved viscosity and foam stability, and it was very unexpected that such a viscosity can be achieved without the addition of any electrolytes and additional stronger thickeners.

An unexpected effect was the fact that the introduction of rheological modifier into the detergent compositions facilitated the formation of intramolecular polymer / surfactant mixtures, which showed a synergistic antimicrobial effect with biocidal additives that are part of the detergent compositions.

In accordance with the invention, the washing composition includes one or more surfactants from anionic and amphoteric surfactants, a rheological modifier in the form of nonionic polymers from the class of cellulose ethers, as well as antimicrobial additives, the activity of which is controlled by a carefully selected composition.

The introduction of an anionic surfactant widely used for these purposes, sodium laureth sulfate, having the formula 0Η ₃ - (0Η2) η- (0-0Η2-0Η ₂ ) ο-0803ΝΗ, where n = 11-15, t = 2, allows to obtain a cheap washing composition with good foaming ability.

Additional introduction of mild amphoteric surfactant betaines of formula KΟ0ΝΗ ₂ (ΟΗ _{2) 3} Ν ^ (ΟΗ _{3) 2} ΟΗ ₂ 000 ^- can significantly reduce the irritant effect on the skin of anionic surfactants increase the dermatological compatibility of the overall system.

If necessary, a further increase in viscosity of the inventive detergent composition is used the fatty acid alkylolamides Β00Ν formula (0Η ₂ 0Η ₂ 0Η) _2, oxides of amines of formula Κ ^{ι Κ:} Κ ³ Ν0 and sodium chloride, which also control the dispersibility and rheological properties of the foam.

The use of pearlescent additives in the form of thin emulsions of glycol monostearates, glycol

- 4 014063 distearates and mixtures thereof allows to obtain a detergent composition with a pleasant pearlescent shade.

Using a natural antiseptic in the form of tea tree oil allows you to get a detergent composition for washing skin and hair with good bacteriostatic activity in a relatively wide range of microorganisms.

Individually, tea tree oil is a good antiseptic. It was unexpected that the desired antimicrobial activity of the claimed detergent composition with tea tree oil was achieved at a much lower concentration than in the case of using tea tree oil individually. In addition, tea tree oil alone can cause allergic reactions of the respiratory tract and skin irritation. In the inventive detergent composition, such negative aspects are eliminated, as indicated by an increase in YO _{50 of the} claimed detergent composition by more than 6 times in comparison with the HO _{50 of} pure tea tree oil.

The introduction of a biocidal component from the group of nitrogen-containing compounds in the form of Ν, бис-bisDZaminopropyl) dodecylamine allows one to obtain a detergent composition for hygienic washing of skin with high antimicrobial activity. From literature data it is known that Ν, Ν-bis (3-aminopropyl) dodecylamine in an individual state has a wide spectrum of antimicrobial activity and is used as an additional active substance in some disinfectants intended for disinfecting surfaces of premises, furniture, utensils, equipment, products medical purposes (Kardash G.G., Belyakov A.I., Pomogaeva L.S., Moroz A.F. Tasks of modern disinfection and ways to solve them: Materials of the All-Russian scientific conference dedicated to 70 years Jun NID MH RF, 2003). The use of Ν, Ν-bisDZ-aminopropyl) dodecylamine in skin antiseptics is very insignificant due to its high toxicity: oral toxicity of LE _5o (rats) - 280 mg / kg and dermal toxicity: LO ₅₀ (rats)> 600 mg / kg.

The unexpected result was that in the claimed washing composition Ν, Ν-bisDZaminopropyl) dodecylamine, without introducing any additional widely used antimicrobial substances for these purposes, not only did not lose its biocidal properties, but even vice versa - due to the synergistic effect, the whole composition acquired a virucidal effect in relation to hepatitis A. The even more unexpected and important for the claimed detergent composition was the fact that the acute toxicity of the product was reduced by more than 75 times, which, probably, can be explained by m of rheological modifier from the class of cellulose ethers, i.e. the detergent composition has become dermatologically acceptable for frequent use in disinfection of the skin.

Suitable antimicrobial additives for use in the detergent composition of the present invention are antimicrobial agents, for example 2-bromo-2nitropropanediol-1,3 (bronopol), methylparaben or propylparaben, or mixtures thereof. To make the right choice from the whole variety of antimicrobial additives for washing compositions is a very difficult task, since it is necessary to conduct studies on the effect of all the ingredients included in the composition on the effectiveness of the selected antimicrobial additive. So, for example, from literature data it is known that anionic surfactants exhibit weak antimicrobial properties, destroying the lipid layers of bacterial cell membranes, however, many anionic surfactants have an antagonistic effect on the antimicrobial properties of parabens when they are present together Ekup, 1990). An unexpected and surprising fact was the increase in the antimicrobial activity of parabens in the presence of sodium laureth sulfate. Moreover, each component separately does not have such a high antimicrobial activity as in the case of their joint presence.

The use of polyethylene glycol, or propylene glycol, or glycerol reduces the resorption and irritating effect of anionic surfactants on the skin, and moisturizes the skin surface for a long time. In this case, preferably, compounds of the class [-CH ₂ CH ₂ O-] _p N, where p = 6-20, are used as polyethylene glycol.

Under the influence of the therapeutic agent used in the claimed invention in the form of 5 ureidohydantoin (allantoin), which has a keratolytic effect, the cells regenerate due to their proliferation, soften and moisturize the skin. The main therapeutic effect of 5ureidohydantoin is a powerful stimulation of the regenerative effect and in enhancing tissue repair.

The additional introduction of organic acid in the form of citric acid and in the form of sodium salt of acetic acid allows you to adjust the pH of the washing composition.

The additional introduction of a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid reduces the hardness of the water used, which in turn allows the use of water of any hardness for the preparation of compositions.

The additional introduction of a mixture of aromatic substances with a characteristic odor allows you to give the detergent composition a pleasant aroma, thereby improving the consumer properties of the final product.

The inventive washing composition according to claims 1 to 6 of the claims is ready to

- 5 014063 application of the product in the form of a gel-like mass without impurities of light yellow color (or white with a pearlescent shade) with the smell of perfume used, with a pH of 5.0-7.0, with a foaming ability in the range of 160-190 mm and stability foam not less than 0.8.

The acute oral toxicity of the detergent compositions according to claims 1-6 is studied in white non-linear rats when introduced into the stomach. Signs of intoxication and changes in animal behavior were not observed. The death of experimental animals during the observation period after administration was not observed. It was found that L0 ₅₀ when introduced into the stomach is more than 5500 mg / kg, i.e. according to GOST 1.12.007-76 belongs to the 4th class of low-hazard compositions. To assess the effect of the detergent composition on the skin condition on clipped skin of the skin, backs of white rats (application area 4 cm ² ) were applied for 15 min in an amount of 20 mg / cm ³ 3-fold applications of native preparations for 3 days. External signs of skin irritation (erythema and edema) were absent. The skin remained smooth, supple, did not lose its natural color. During the entire observation period, the experimental animals remained active and ate well-fed food. Index of local irritating effect on the skin of animals I si! = 0.

When determining the sensitizing ability of the test, the test was performed by setting up a closed epicutaneous patchwork sample (sample 1-2) on a group of eight volunteers for 5 days. Studies included the application of primary sensitizing and secondary provocative applications on the inner surface of the forearm of volunteers. In subjects exposed to the study, subjective symptoms (sensation of itching, burning, etc.) and objective signs of skin irritation at the site of the primary and secondary applications were not detected. Detergent compositions did not have sensitizing activity, 18 = 0.

When assessing the effect of the detergent composition on the state of the mucous membranes, single instillations of 25 μg of preparations (described in claims 3-6) were performed in the form of 50% solutions in the lower conjunctival arch of guinea pigs. It was found that as a result of the experiments there is a slight lacrimation, ptosis, which takes place within 3-5 minutes after administration. There were no visible signs of irritation of the mucous membranes of the eyes of laboratory animals 24 hours after the start of the study. Organic violations of the integrity of the mucous membranes when they were detected under conditions of supravital staining with a 2% fluorescent solution using a slit lamp were not detected, Ig = 0.

During the trial application of preparations within 3 days by volunteers for the purpose of the phenomena of irritation (hypermia, dryness and peeling) of intact skin in the places of use was not observed.

The inventive washing composition according to claims 7, 8 of the claims is a ready-to-use product in the form of a gel-like mass without extraneous impurities of a light yellow color with the smell of tea tree oil, with a pH of 5.0-7.0, with foaming ability within 160 -180 mm and foam resistance of at least 0.8.

The inventive washing composition according to claims 9, 10 of the claims is a ready-to-use product in the form of a gel-like mass without extraneous impurities of a light yellow color with a characteristic odor of the perfume used, with a pH of 5.0-7.0, with a foaming ability in the range of 100 -130 mm and foam resistance of at least 0.8.

The Central Scientific Research Laboratory of the Belarusian Medical Academy of Postgraduate Education conducted toxicological tests of detergent compositions according to claims 710 of the claims. Processing of the obtained data was carried out on a Ce1egop-433 t personal computer software package М1сго8ой ОГПсе. Statistical processing was performed using the Excel spreadsheet program and the Asse8 spreadsheet program. Assessment of the statistical significance of the indicators was carried out using the Student's test. Differences were considered significant at p <0.05 and highly reliable at p <0.01.

When conducting toxicological tests of the detergent composition according to claims 7-10, the following methodological recommendations were used: Temporary methodical recommendations Requirements for preclinical study of the general toxic effect of new pharmacological substances. Moscow, 1985; Guiding regulatory document Rules for preclinical safety assessment of pharmacological agents (СР), Moscow, 1992; Methodical instructions. Research design in the scope of the initial toxicological assessment of substances, Minsk, 1994.

The acute toxicity of the detergent compositions according to claims 7-10 was studied in white outbred mice and rats. The test detergent composition was administered intragastrically once. Experimental animals were observed for 14 days. During this period of time, the general condition of the animals, their behavior, the intensity and nature of motor activity, the presence and nature of seizures, coordination of movements, skeletal muscle tone, response to tactile, pain, sound and light stimuli, the condition of the hair and skin, and color were evaluated mucous membranes, tail position, feed and water intake. As a result of the studies, ΕΌ ₅₀ values of the detergent compositions according to claims 7-10 were established, which are equal to 12003 mg / kg and 21198 mg / kg, respectively, which is almost 2.5 and 4 times respectively

- 6 014063 maximum permissible value (5000 mg / kg). Irritation of the skin under the action of surfactant solutions is complexly associated with the structure, with the surface and solubilizing activity of surfactants, and can be felt as slight discomfort (excessive dryness of the skin) and as obvious finely lamellar peeling, redness, erythema, infiltration and cracking (M.Yu. Pletnev Cosmetic and hygienic detergents, Moscow, 1990).

It was unexpectedly found that the addition of a rheological modifier in the form of a nonionic polymer is able to significantly change the quality of the formulations of the claimed detergent composition with a simple combination of traditionally used surfactants. With the introduction of a nonionic polymer, it is possible to obtain an inexpensive, well-balanced detergent composition with optimal characteristics both in the quality of the foam and in its consistency, as well as in dermatological properties.

It was also an unexpected fact that the presence of a rheological modifier in the form of a nonionic polymer in the formulations of the claimed detergent composition not only affects the micellar properties of individual surfactants, but also allows the formation of various surfactant mixtures in the system of microstructures, which has a decisive effect on the stability of the detergent composition, containing tea tree oil, without the use of additional structural components.

The introduction of a natural antibacterial additive in the form of tea tree oil into the detergent composition made it possible to obtain a detergent composition with a bacteriostatic effect, which is very important when using it to disinfect skin. Even more unexpected was that at the same time it can be used as a cosmetic composition for skin and hair care, which normalizes the natural balance and has a beneficial effect on the whole organism.

The inventive detergent composition according to the invention can be obtained by introducing in any order the calculated amount of ingredients into water (tap water or purified at a temperature not exceeding 40 ° C), followed by stirring until a homogeneous mass is obtained. The mixing time depends on the volume of the reactor, the speed and configuration of the mixer. The disadvantage of this method is that the mixing time of the entire mass is governed by the time of hydration and dissolution of the thickener to form a homogeneous mass without impurities. During such mixing, it is very difficult to take into account all the thermodynamic parameters of the system as a whole, since unpredictable competing interactions of the ingredients in the mixture are possible due to both chemical and physical factors, which ultimately will have an adverse effect on the final product.

Another aspect of the present invention is a method for preparing the inventive detergent composition, which overcomes the above problems. In accordance with the inventive method, pre-hydration and dissolution of the thickener are carried out in a separate mixer with heating to a temperature not exceeding 50 ° C or without heating the entire system and then the resulting solution at a temperature not exceeding 40 ° C is mixed in any order with the estimated amount of antimicrobial surfactants agents, citric acid, and targeted additives, administered alone or in any combination, and mix the mixture until a homogeneous mass is obtained.

The time of hydration and complete dissolution depends on the concentration of the rheological modifier, water temperature, and mixing speed. Subsequent mixing of all ingredients is carried out in any sequence and in any combination, taking into account the dissolved modifier and taking into account the specified rheological properties of the final product, since the latter is determined not only by the qualitative and quantitative composition of the formulation, but also by the technological equipment on which it is produced. This is especially necessary to take into account when preparing a detergent composition, which includes a rheological modifier - a polymer thickener. The mixing process is carried out with constant stirring. Important parameters of the method are the mandatory constant mixing until all components are completely dissolved, the speed and configuration of the mixer. The loading system, configuration, and speed of the mixing device make it possible to obtain a stable, viscous washing composition without vigorously foaming the foam in the reactor.

The following examples allow you to get acquainted with the invention without limiting its scope.

In the table. 1 presents data confirming the claimed ratio of the components of the detergent composition according to claims 1 to 10 of the claims.

The process of preparing a detergent composition for disinfection of the skin includes preparing equipment and raw materials, weighing the components and loading them, intermediate control of the intermediate and the finished product at each stage of the technological process.

Example 1

Stage I. To 400 kg of water without heating or with heating to a temperature of no higher than 50 ° C in a separate mixer with constant stirring, add 4.0 kg of hydroxyethyl cellulose and mix until it is completely dissolved.

Stage II. In any order of entry, the rheological modifier prepared in stage I is mixed with 490 kg of an aqueous solution of sodium laureth sulfate (10.7%), 5.0 kg of trilon B, 35.0 kg of fatty acid diethanolamides, 25.0 kg of polyethylene glycol, 15, 0 kg of pearlescent additives, 0.8 kg of citric acid, 0.1 kg of bronopol, 3.0 kg of perfume fragrance, 15.0 kg of sodium chloride. All components add

- 7 014063 into the reaction mixture with the stirrer constantly on. Each subsequent component is added after complete dissolution of the previous one. The mixture was adjusted with water to a total weight of 1000 kg. After 3-4 hours, the stirrer (mixing time depends on the configuration and speed of the stirrer) is turned off and the product is left in a container for settling foam (if the characteristics of the technological equipment do not allow the formation of foam). If the processing equipment allows you to get the product with stirring without the formation of foam, then the product can be poured into consumer containers immediately after obtaining a homogeneous stable viscous mass.

Example 2

The method is carried out as described in example 1, only in stage II, instead of a pearlescent additive, 25.0 kg of betaine is introduced.

Example 3

Stage I. To 600 kg of water without heating or with heating to a temperature not exceeding 50 ° C in a separate mixer with constant stirring, add 6 kg of hydroxyethyl cellulose and mix until it is completely dissolved.

Stage II. In any order of entry, the rheological modifier prepared in stage I is mixed with 257 kg of an aqueous solution of sodium laureth sulfate (2.7 wt.%), 7.0 kg of trilon B, 1.5 kg of fatty acid diethanolamides, 5.7 kg of betaines, 7.0 kg of allantoin, 9.0 kg of tea tree oil, 0.8 kg of citric acid, 15 kg of a mixture of 7.6 kg of metiparaben, 2.4 kg of propyl paraben and 5.0 kg of polyethylene glycol. All components are added to the reaction mixture with the stirrer constantly on. Each subsequent component is added after complete dissolution of the previous one. Bring water to a total weight of 1000 kg. After 3-4 hours, the stirrer (mixing time depends on the configuration and speed of the stirrer) is turned off and the product is left in a container for settling foam (if the characteristics of the technological equipment do not allow the formation of foam). If the processing equipment allows you to get the product with stirring without the formation of foam, then the product can be poured into consumer containers immediately after obtaining a homogeneous stable viscous mass.

Example 4

Stage I. To 800 kg of tap water without heating or with heating to a temperature not exceeding 50 ° C in a reactor with a capacity of up to 2000 l with constant stirring, add 40 kg of hydroxyethyl cellulose and mix until it is completely hydrated and dissolved.

Stage II. In any convenient order of input, the rheological modifier prepared in stage I is mixed with 45.0 kg of betaines, 3.5 kg of trilon B, 5.0 kg of allantoin, 18.0 kg of amine oxide, 18.0 kg Ν, Νbis- (3 -aminopropyl) dodecylamine, 57.0 kg of sodium acetate, 5.5 kg of citric acid. All components are added to the reaction mixture with the stirrer constantly on. Each subsequent component is added after complete dissolution of the previous one. The entire contents of the reactor are mixed until a homogeneous transparent viscous mass is formed. Then, with constant stirring and cooling (if the technological process included the stage of heating the water), add 5.0 kg of perfume fragrance. The contents of the reactor with constant stirring was adjusted with water to a total mass of 1000 kg. After 3-4 hours of stirring, the product can be poured into consumer containers.

The antimicrobial activity of the experimental samples of the claimed detergent composition according to claims 7, 8 of the claims was studied by методамиη νίίτο by suspension methods and ίη νίνο for hygienic hand antiseptics according to MU No. 11-13-197 Methodological instructions. Test methods for the antimicrobial activity of prophylactic antiseptics (the results are shown in table. 2).

When assessing the bactericidal and fungicidal activity of the detergent composition by a quantitative suspension method, the daily microbial cultures of test microbes were weighed in physiological saline with 20% horse serum and standardized to 10 ⁹ CFU / ml and mixed in a ratio of 1:10. At the expiration of exposure 0.5; one; 3; 5 last neutralized. From the neutralizer solution, 0.1 ml were sown on the sectors of the cups with the corresponding test microbes, selective or differential diagnostic nutrient media. For control, the experiment was repeated using sterile distilled water instead of the washing composition. Crops were incubated for 48 hours. The number of colonies was counted and the number of surviving bacteria (CFU / ml) was determined in the experiment and control. Decimal logarithms and reduction factors (EB) of the number of bacteria in the experiment were determined in comparison with the control. The results were taken into account by the presence or absence of growth in the experiment in the presence of growth in the control.

As a result of studies, it was found that the detergent composition in the quantitative suspension method causes a decrease in the number of viable cells of a typical E. coli culture from 1.86 to 3.21 1d for 1-5 minutes. In relation to staphylococcus, the level of antimicrobial activity of the detergent composition (EB) increased from 1.86 1d to 3.25 1d within 1-5 minutes.

In relation to the typical culture of Pseudomonas aeruginosa, reduction factors increased from 1.72 1d to 3.4 1d within 1-5 minutes.

The results of studies of the bacteriostatic and fungistatic activity of the detergent composition, studied according to the above method without neutralizing the active substance, are given in table. 2.

When evaluating the antimicrobial activity of the claimed detergent composition ίη νίνο hygienic we

- 8 014063 hands were performed as follows: the hands contaminated with the culture of E. coli were moistened with running water and treated with 3 ml of the washing composition for 60 s. The results according to the degree of activity were evaluated as a reduction factor (KE), which are presented in table. 3.

Criteria for assessing the antimicrobial activity of skin antiseptics according to MU No. 11-13-197 Guidelines. The test methods for the antimicrobial activity of prophylactic antiseptics are as follows.

A detergent in various forms for hygienic treatment is considered effective if the reduction factor> 2 1d in experiments ίη νίνο.

In the table. 4 presents an assessment of the antimicrobial activity of the detergent composition with hygienic hand antiseptics. From the table. 4 it is seen that the average indicator of the reduction factor of the test culture of Escherichia coli on the skin of the hands of the testers under the action of the inventive antiseptic composition for 60 s was 3.26 ± 0.09.

The antimicrobial activity of the experimental samples of the claimed detergent composition according to claims 9 to 10 of the claims was also studied by ίη νίίτο by suspension methods and ίη νίνο for hygienic hand antiseptics according to MU No. 11-13-197 Methodological instructions. Test methods for the antimicrobial activity of prophylactic antiseptics (the results are shown in table. 5).

The detergent composition according to claims 9-10 in the quantitative suspension method causes a decrease in the number of viable cells of a typical strain of E. coli culture by 5.0 1 d or more under all the studied modes.

In relation to the typical culture of Z. aigeik, the level of bacterial activity of the detergent composition (KE) increased from 5.22 1d at 25.0% concentration at an exposure of 30 s to 6.62 1d at 100% concentration at an exposure of 1 min.

In relation to the typical culture of R. aegsdshock, the reduction factor increased from 3.84 1d at 25% concentration at an exposure of 30 s to 6.82 1d at 100% concentration at an exposure of 1 min.

For a typical C. a1b1cap8 culture, the reduction factor ranged from 3.36 1d at 25% concentration to 5.34 1d at 100% concentration with an exposure of 1 min.

When evaluating the antimicrobial activity of the detergent composition according to claims 9, 10 of the claims with hygienic hand antiseptics, the studies were performed ίη νίνο. The method of antiseptic processing of the hands was as follows: the hands were washed with ordinary soap or with the claimed detergent composition according to claims 3-6 under warm running water for 2 minutes, dried using disposable wipes. The fingers were contaminated with a suspension of E. coli test culture in saline with 20% horse serum with a density of 10 CFU / ml, and dried in air. The skin of the hands of the first group of testers was treated with 3 ml of the claimed detergent composition according to claims 9, 10 for 30 seconds.

The second group of testers handled the hands in this way: the skin of the hands was treated with 3 ml of the claimed detergent composition according to claims 9, 10 of the claims for 30 s, washed off with running water. The procedure was repeated twice. To determine the number of microbes on the skin of the hands of the testers before and after the antiseptic treatment of the hands with the claimed detergent composition according to claims 9, 10 of the claims, finger washings were carried out in a solution of a neutralizer followed by inoculation of washing liquid onto nutrient media.

The results of studies of the antimicrobial activity of the claimed composition according to claims 9, 10 of the claims carried out in the second group of testers are presented in table. 6.

Reduction factors for the test culture of Escherichia coli during hygienic washing of hands using the claimed detergent composition according to claims 9, 10 of the claims with a single use for 30 s were less than 4 1 d and ranged from 3.07 to 4.41 1 d for different testers. In the repeated method of treatment (twice in 3 ml for 30 s), the reduction factors of E. coli ranged from 4.72 to 6.31 1d in different testers, averaging 5.51 1d.

Based on the literature data (Topu O'Beshsk, 8b1es11bc, Avb Baiga Labeimi. SohteTs & ΤοίΕΐ ^ δ. Magsy, 2006) it is known that washing compositions for hygienic skin and hair care are a mixture of various surfactants and additional ingredients, in which complex physical and chemical interactions occur. Surfactant mixtures are used to achieve one or another qualitative indicator of a product if it is impossible to achieve it using only one surfactant. A non-additive change in any indicator is evidence of synergism or antogonism between surfactants that are in the same system. In the literature (Lyuv Ζί ^ πιτιη Müe11ag 51gis1ige anb Γ1κο1ο§χη1 еййамюга ίη пихеб асГГЭГЭНИ вуйетк), the influence of the formation of the most thermodynamically probable microstructures on the rheological properties of a system of two different surfactants is described, the formation of which determines synergism or However, the picture is greatly complicated when more than two ingredients are introduced into the system, especially if various rheological modifiers in the form of polymers are introduced into the system.

The most unexpected positive effect of the preferred embodiment of the claimed composition was that, using I, I-bis- (3-aminopropyl) dodecylamine as a biocide

- 9 014063 with a rheologically modified system of various surfactants and additional appropriate ingredients, it is possible to obtain a dermatologically mild detergent composition with antimicrobial properties, not inferior in effectiveness and superior in virucidal activity to existing and widely used indelible antiseptic compositions for disinfection of skin integuments. So, the average values of the reduction factor of the test culture of Escherichia coli with hygienic antiseptics of the hands of the testers for the claimed detergent composition and for comparison, 60 vol.% Isopropyl alcohol (control) were very close and amounted to 5.25 and 5.74 1d, respectively. Additionally, the claimed washing composition has antimicrobial activity against mycobacteria and, very unexpectedly and very topically, has virucidal activity against hepatitis A.

The positive effect of the concept of modifying the rheology of the washing composition using a non-inogenic polymer in the form of cellulose ethers is:

improving the rheological properties of the washing composition;

optimization of the cleaning, moisturizing and antimicrobial properties of the detergent composition; the variety of consumer properties of the washing composition, which has excellent compatibility with the skin and a high spectrum of antimicrobial activity;

expanding the range of products for disinfection of the skin;

the creation without additional introduction of structuring ingredients of the microstructure of the detergent composition with a bactericidal action based on a natural antimicrobial additive;

offering as an alternative to skin antiseptics used by rubbing, a highly effective detergent composition that meets all regulatory requirements for skin disinfectants used for hygienic and surgical hand antiseptics of medical personnel;

obtaining high-quality inexpensive detergent composition through the use of inexpensive surfactants and small amounts of natural rheology modifier.

Table 1

Ingredients The amount of the ingredient in the recipe, wt.% In 100 g one 2 3 4 5 6 7 8 nine Sodium Laureth Sulfate 2.0 3,5 7.0 7.0 7.7 8.5 8.6 10.0 12 Diethanolamide 7.0 2.0 2,5 3.3 2,5 7.5 2,4 1,5 0.5 Betaines 7.0 0.7 - 2.9 - 10.0 - 4.0 7.5 Nacre 4.0 - 1,5 0.75 1.25 - 1,04 - - Amine oxide Allantoin - 0.05 - 0.12 - 1,0 - 1,0 - KM-bis (bis-aminopropyl) dodecylamine PEG - 1,0 1,0 2.0 1,2 10.0 1,2 10 - Tea tree oil - 0.25 - 0.5 - 1,5 - 0.05 - Glycerol 5,0 - - - - - - - 10.0 Propylene glycol Rheological modifier 1,0 0.16 0.35 0.33 0.20 3,5 0.23 5,0 4.0 Propylparaben 0.4 0.16 - 0.31 - 0.005 - 0.8 Methylparaben 0.4 0.23 - 0.45 - 0.9 - 0.8 0.05 Trilon B 0,085 0.1 0.15 0.08 0.5 0.1 1,0 Bronopol 0.01 - 0,075 - 0.1 - 0.08 - 0.05 Sodium Acetate - Sodium chloride 1,2 - 1.8 - 2,3 - 2,3 - 0.01 Citric Acid 0.4 0,035 0.06 0,07 0.08 0.5 0,07 0.9 0.8 Perfume - - 0.09 - 0.08 0.09 0.5 Water OST. OST. OST. OST. OST. OST. OST. OST. OST

- 10 014063

Ingredients The amount included in the recipe ingredient, wt.% 100 g 10 eleven 12 thirteen 14 fifteen sixteen 17 eighteen Sodium Laureth Sulfate 1,0 3,5 M 6.4 6.4 7.0 7.0 7.0 7.0 Diethanolamide 1,0 1,0 1,5 1.8 3.4 4.4 0.5 2.2 0.5 Betaines 10 10.0 5.2 0.54 1,1 1.4 3,5 0.67 2,5 Nacre - 0.5 - - - - 1,0 - - Amine oxide - 0.5 8.0 - - - 5.7 - 3.0 Allantoin 1,0 0.1 0.3 - 0.12 0.10 0.7 - 0.2 Ν, Ν-5ηο (6ηο-3ΜΗΗΟπροπηλ) dodecylamine - 2.0 0.75 - - - 0.5 - 1,5 PEG - - - 1,6 2.0 2.0 - 2,4 - Tea tree oil 0.05 - - - 0.5 0.5 - - - Glycerol - - - - - - - - - Propylene glycol 10.0 - - - - - - - 3.0 Rheological modifier 0.01 1,5 2.0 0.10 0.25 0.32 4.7 0.23 5,0 Propylparaben 0.05 - - - 0.32 0.32 - - - Methylparaben 0.5 - - - 0.45 0.46 - - 0.005 Trilon B 0.2 0.3 0.08 0.15 0.17 1,0 0.08 0.2 Bronopol - - - 0.08 - - - 0.08 - Sodium Acetate - 1,0 10.0 - - - 1,0 - 10.0 Sodium chloride - - - 2.23 - - - 1.95 - Citric Acid oh 1 0.5 0.1 0.05 0,07 0,07 0.1 0.06 0.05 Perfume 0.25 0.12 0.10 - - 0.3 0.1 0.05 Water rest OST. OST. rest rest OST. rest OST. rest Ingredients The amount included in the recipe ingredient, wt.% 100 g nineteen twenty 21 22 23 24 25 26 27 Sodium Laureth Sulfate 1,0 7.03 7.4 7.4 7.4 7.4 10.5 10.7 eleven Diethanolamide 4,5 1.35 0.5 2.0 1,5 2.12 10 1,5 6.0 Betaines 7.2 - 10 4.0 3,5 - 0.1 1.8 1.8 Nacre - 0.77 1.5 1,0 - 0.9 0.2 - - Amine oxide - - - - - - - 4.7 - Allantoin 0.5 - - 1,0 - - 1,0 1,0 MDC bis (bis-aminopropyl) dodecylamine - - - - - - - 0.01 - PEG - 1,03 2.0 - - 1,2 - - 2.2 Tea tree oil - - - 0.3 - - 1,5 - 0.5 Glycerol 8.5 - - - - 10.0 oh 1 - Propylene glycol - - - 2.0 5,0 - - 0.1 - Rheological modifier 5,0 0.10 1,0 0.23 2.0 0.15 5,0 0.95 0.28 Propylparaben 0,07 - - 0.01 - - 0.8 0.45 0.22 Methylparaben 0.10 - - 0.79 oh 1 - 0.005 0.45 0.45 Trilon B 0.85 0.08 0.1 0.25 0.7 OD 1,0 0.05 0.22 Bronopol 0.001 0.01 0.1 - 0.01 0,07 - - - Sodium Acetate - - - - - - - 0.01 - Sodium chloride 0.95 2,5 - - 0.5 2,5 - - - Citric Acid 0.02 0.06 0.1 0.1 1,0 0,07 1,0 0.73 0.1 Perfume 0.08 0.01 - OD 0.09 - 0.30 - Water OST. OST. rest rest OST. OST. rest OST. rest

- 11 014063

Ingredients The amount included in the recipe ingredient, wt.% 100 g 28 29th thirty 31 32 33 34 35 36 Sodium Laureth Sulfate - - - - - and 12 12 sixteen Diethanolamide - - 5.9 - - 1,5 0.5 2.7 0.5 Betaines 1,1 7.9 0.1 1.3 0.65 2,5 7.5 4.0 - Nacre - 5,0 - - - 1,5 - 2,5 5,0 Amine oxide 1,1 0.1 7.3 0.90 0.45 - - - - Allantoin 0.2 1,0 0.7 0.20 0.1 - - - - M, Y-bis (bis-aminopropyl) dodecylamine 0.6 1,5 1.8 0.5 0.25 - - - - PEG - - - - - 1,5 - 2,5 - Tea tree oil - - - - - - - - - Glycerol - 4.7 - - - - 10.0 - - Propylene glycol - - 0.9 - - - - 10 Rheological modifier 1,0 5,0 2.9 1,0 0.5 0.30 4.0 1,0 1,5 Propylparaben - - 0.05 - - - - - 1,0 Methylparaben - 0.25 0.27 - 0.05 - 0.1 Trilon B 0.2 1,0 0.8 0.1 0.05 0.3 1,0 0.1 Bronopol - - - 0.08 0.05 0.1 0.01 Sodium Acetate 2,3 7.1 5.7 0.8 0.45 - - - Sodium chloride - - - - - 1.9 0.01 1,0 0.85 Citric Acid 0.42 0.67 0.01 0.32 0.16 0,07 0.8 0.15 1,0 Perfume 0.19 0.5 0.01 0.12 0.06 0.1 - 0.5 Water OST. OST. rest OST. rest rest rest rest OST.

table 2

Bacteria NOT at different concentrations of tea tree oil in the claimed detergent composition for hygienic washing of the skin 0.5% 0.375% 0.25% e.soI 1 minute 2,911 ₂ 2,991§ 3.21 18 2 minutes 2.83 1 ₂ 2.88 18 3.09 18 3 minutes 1,861 ₈ 2.34 18 2.8818 B. aigeez 1 minute 2.45 1 ₈ 2.95 1 _y 3,251 ₈ 2 minutes 1,961 ₈ 2.74 1§ 2.95 18 3 minutes 1,861 ₈ 2.13 18 2.85 18 R.aegts§1po8a 1 minute 2.72 1 _e 2.93 1 _y 3,4018 2 minutes 1.9418 2.71 18 2.85 18 3 minutes 1,721® 1,781 _y 2.73 18 S.a1Ysap5 1 minute 1.79 1 ₈ 2.31 18 2,3618 2 minutes 1.3718 1,741 _β 2.2618 3 minutes 0.69 1§ 1.31 18 1,601e

- 12 014063

Table 3

KG at various concentrations of tea tree oil in the inventive detergent composition for hygienic washing

0.5% 0.375% 0.25% E.soP 1 minute 3,541§ 3.76 18 4.65 18 2 minutes 2,891 _e 3.43 1 _y 3.95 1 _y 3 minutes 2.63 1 ₈ 2.71 1 ₈ 3.67 1 ₈ Z. aigeez 1 minute 3,291 _y 4,021§ 4.44 18 2 minutes 2.95 1 ₈ 3,641§ 4.02 1§ 3 minutes 2.1718 3,001 _g 3.92 1§ R. aepshtose 1 minute 3.2718 3.93 18 4.28 18 2 minutes 2.9718 3,1018 4.08 18 3 minutes 2,5718 2.93 1 _y 3,9018 S.a1Ysapz 1 minute 2.93 18 3,2018 3.8418 2 minutes 2.25 1 _e 3.01 18 3.22 1 ₈ 3 minutes 1.99 18 2.4418 2.97 1 _§

Table 4

Testers 3 ml washing composites for 60 sec. before processing 18 after treatment KG one L 7.26 3.98 3.28 P 7.52 3.85 3.67 2 L 7.30 4.00 3.30 P 7.37 4.35 3.02 3 L 7.37 4.01 3.36 P 7.22 4.22 3.00 4 L 7.04 3.56 3.48 P 6.77 3.70 3.07 5 l 7.32 4.00 3.32 P 7.53 4.43 3.10 6 l 7.18 3.72 3.46 P 7.05 3.83 3.22 7 l 6.95 3.49 3.46 P 6.79 5.9 3.77 8 l 7.34 4.0 3.34 P 7.32 4.32 3.00

X = 3.26 ± 0.09

Note: L - left hand; P is the right hand.

- 13 014063

Table 5

Bacteria KG OSR of various concentrations of biocidal additives in the inventive detergent composition for hygienic washing of the skin 0.5% 0.375% 0.25% E.coN 0.5 minutes 5.84 1 _yo 5,8018 5.8218 1 minute 6.84 1§ 6.83 18 6.83 18 2 minutes 6.83 18 6.83 18 6.83 18 5 minutes 6.83 18 6.83 18 6.83 18 B. aiteiz 0.5 minutes 5.80 18 5.22 1 ₈ 5.22 1 ₈ 1 minute 6.62 18 5.60 18 5.22 18 3 minutes 6.62 18 5.62 18 5.22 18 5 minutes 6.62 18 5.65 1§ 5.22 18 R.aegtsgtosa 0.5 minutes 5.9118 4.74 18 3.84 18 1 minute 6.82 18 4.89 18 4,55 1§ 3 minutes 6.82 18 5.53 18 4.7718 5 minutes 6.82 18 5.53 1 ₈ 5.52 1 _y S.aImsapb 0.5 minutes 4.711 ₈ 3.97 1c 3.3618 1 minute 5.3418 4,3018 3.82 18 3 minutes 5.34 18 4,58 1 ₈ 4.30 18 5 minutes 5.3418 5,341c 4.68 1§

Table 6

Testers Before processing After processing KG eighteen B8 one L 7.36 2.60 4.76 P 7.32 2.60 4.72 2 l 7.46 1.77 5.69 P 7.46 1.77 5.69 3 l 7.61 1.30 6.31 η 7.50 1.30 6.20 4 l 7.52 1,60 5.92 P 7.45 1.30 6.15 5 l 7.07 2.04 5.03 P 7.32 2.00 5.32 6 l 7.51 2.00 5.51 P 7.68 2,36 5.32 7 l 7.83 2.77 5.06 P 7.53 2,52 5.01 8 l 7.49 1.77 5.72 P 7.63 1.95 5.68 X = 5.51 ± 0.08

Note: L - left hand; P is the right hand.

Claims (11)

1. A water-based detergent composition for skin disinfection, containing one or more surfactants, antimicrobial agents, citric acid and, if necessary, target additives, characterized in that it further comprises a rheological modifier selected from the group comprising methyl cellulose, methyl ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, and as a surfactant contains together or separately sodium laureth sulfate and betaines, as antimicrobial agents with holds in combination or separately 2-bromo-2-nitropropanediol-1,3, methylparaben, propylparaben, tea tree oil, Ν, Ν-bis (3-aminopropyl) dodecylamine in the following ratio, wt.%: Surface-active substance 0.1-26.0 Rheological modifier 0.01-5.0 Antimicrobial agent 0.001-2.0 Citric Acid 0.01-1.0 Targeted Supplements 0.01-27.0 Purified or Drinking Water up to 100 2. The washing composition according to claim 1, characterized in that, as target additives, it contains, together or in combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerol, or propylene glycol - 0.1-10.0 wt.%, Fatty acid diethanolamides - 0.5-10.0 wt.%, Sodium chloride - 0.01-5.5 wt.%, Amine oxides 0.1-8.0 wt. %, allantoin - 0.05-1.0 wt.%, sodium acetate - 0.01-10.0 wt.%, perfume fragrance - 0.010.5 wt.%. 3. The detergent composition according to claim 1, characterized in that it is a liquid soap, contains sodium laureth sulfate and betaine as a surface-active substance, and contains 2-bromo-2-nitropropanediol-1 in combination or separately as antimicrobial agents, 3, methylparaben, propylparaben in the following ratio of components, wt.%: Sodium Laureth Sulfate 1-16,0 Betaines 0.1 -10.0 Rheological modifier 0.01-5.0 Antimicrobial agent 0.001-1.0 Citric Acid 0.01-1.0 Targeted Supplements 0.01-27.0 Purified or Drinking Water up to 100 4. The detergent composition according to claim 1, characterized in that, together with or in combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerol, or propylene glycol, contains, as target additives - 0.1-10.0 wt.%, Diethanolamides of fatty acids - 0.5-10.0 wt.%, Sodium chloride - 0.01-5.5 wt.%, Perfume fragrance - 0.01-0, 5 wt.%. 5. The washing composition according to claim 1, characterized in that it is a liquid soap and further comprises glycol monostearates or glycol distearates or mixtures thereof, contains sodium laureth sulfate as a surfactant, and as a antimicrobial agent contains in combination or separately 2-bromo-2-nitropropanediol-1,3, methylparaben, propylparaben in the following ratio of components, wt.%: Sodium laureth sulfate 1 -16.0 Glycol monostearates or glycol distearates or mixtures thereof 0.2-5.0 Rheological modifier 0.01-5.0 Antimicrobial agent Citric Acid 0.001-1.0 0.01-1.0 Targeted Supplements 0.01-27.0 Purified or drinking water up to 100 6. A detergent composition according to claim 3, characterized in that, together with or in combination, a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerol, or propylene glycol, is contained as target additives - 0.1-10.0 wt.%, Diethanolamides of fatty acids - 0.5-10.0 wt.%, Sodium chloride - 0.01-5.5 wt.%, Perfume fragrance - 0.01-0, 5 wt.%. - 15 014063 7. The detergent composition according to claim 1, characterized in that it is a liquid soap and contains sodium and betaine laureth sulfate as surface-active substances, and contains tea tree oil, methyl paraben and / or propyl paraben in the following ratio, wt. .%: Sodium Laureth Sulfate 1-16,0 Betaines 0.1-10.0 Rheological modifier 0.01-5.0 Tea tree oil 0.05-1.5 Methylparaben and / or propylparaben 0.005-1.0 Citric Acid 0.01-1.0 Targeted Supplements 0.01-22.0 Purified or Drinking Water up to 100 8. The detergent composition according to claim 5, characterized in that, as target additives, it contains together or in combination a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Polyethylene glycol, or glycerol, or propylene glycol - 0.1-10.0 wt.%, Diethanolamides of fatty acids - 0.5-10.0 wt.%, Allantoin - 0.05-1.0 wt.%. 9. The detergent composition according to claim 1, characterized in that it is a means for hygienic washing of the skin and as a surfactant contains betaines, as an antimicrobial agent S, M-bis- (3-aminopropyl) dodecylamine in the following ratio components, wt.%: Betaines 0.1-10.0 Ν, Ν-bis- (3-amino lropyl) dodecyl l amine 0.01-2.0 Rheological modifier 0.01 - 5.0 Citric Acid 0.01-1.0 Targeted Supplements 0.01-20.5 Drinking water up to 100 10. The washing composition according to claim 7, characterized in that, as target additives, it contains together or in combination a chelating compound in the form of sodium salts of ethylenediaminetetraacetic acid in an amount of 0.01-1.0 wt.%, Amine oxides - 0.1-8 , 0 wt.%, Allantoin - 0.05-1.0 wt.%, Sodium acetate - 0.01-10.0 wt.% And perfume fragrance - 0.01-0.5 wt.%. 11. A method of preparing a detergent composition according to any one of claims 1 to 10, comprising pre-hydrating and dissolving the rheological modifier in water at a temperature not exceeding 50 ° C, followed by mixing it at a temperature not exceeding 40 ° C with other components introduced into in any order separately or in combination, and stirring the mixture until a homogeneous mass is obtained. Eurasian Patent Organization, EAPO Russia, 109012, Moscow, Maly Cherkassky per., 2