DK3562941T3 - Fremgangsmåde til generering af genom-editing-biblioteker af højere orden - Google Patents
Fremgangsmåde til generering af genom-editing-biblioteker af højere orden Download PDFInfo
- Publication number
- DK3562941T3 DK3562941T3 DK17832242.6T DK17832242T DK3562941T3 DK 3562941 T3 DK3562941 T3 DK 3562941T3 DK 17832242 T DK17832242 T DK 17832242T DK 3562941 T3 DK3562941 T3 DK 3562941T3
- Authority
- DK
- Denmark
- Prior art keywords
- generation
- genome
- order
- libraries
- editing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/04—Identifying library members by means of a tag, label, or other readable or detectable entity associated with the library members, e.g. decoding processes
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/02—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) hydrolysing N-glycosyl compounds (3.2.2)
- C12Y302/02027—Uracil-DNA glycosylase (3.2.2.27)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y605/00—Ligases forming phosphoric ester bonds (6.5)
- C12Y605/01—Ligases forming phosphoric ester bonds (6.5) forming phosphoric ester bonds (6.5.1)
- C12Y605/01001—DNA ligase (ATP) (6.5.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102016125894 | 2016-12-29 | ||
| PCT/EP2017/084625 WO2018122248A1 (en) | 2016-12-29 | 2017-12-27 | Method for generating higher order genome editing libraries |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK3562941T3 true DK3562941T3 (da) | 2023-03-13 |
| DK3562941T5 DK3562941T5 (da) | 2024-09-02 |
Family
ID=61005785
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17832242.6T DK3562941T5 (da) | 2016-12-29 | 2017-12-27 | Fremgangsmåde til generering af genom-editing-biblioteker af højere orden |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US12091656B2 (da) |
| EP (1) | EP3562941B1 (da) |
| JP (1) | JP7142637B2 (da) |
| CN (1) | CN110249049A (da) |
| AU (1) | AU2017385611B2 (da) |
| CA (1) | CA3048939A1 (da) |
| DK (1) | DK3562941T5 (da) |
| ES (1) | ES2940619T3 (da) |
| WO (1) | WO2018122248A1 (da) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3578658A1 (en) * | 2018-06-08 | 2019-12-11 | Johann Wolfgang Goethe-Universität Frankfurt | Method for generating a gene editing vector with fixed guide rna pairs |
| US20240287506A1 (en) * | 2021-06-21 | 2024-08-29 | Westlake University | Library construction method based on long overhang sequence ligation |
| WO2023108430A1 (zh) * | 2021-12-14 | 2023-06-22 | 中国医学科学院药用植物研究所 | 基于全基因组分析与基因组编辑的植物物种鉴定方法与应用 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6156509A (en) * | 1997-11-12 | 2000-12-05 | Genencor International, Inc. | Method of increasing efficiency of directed evolution of a gene using phagemid |
| AU2001241939A1 (en) * | 2000-02-28 | 2001-09-12 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
| WO2004072244A2 (en) * | 2003-02-06 | 2004-08-26 | Rensselaer Polytechnic Institute | Polymerase-based protocols for generating chimeric oligonucleotides |
| US9476079B2 (en) * | 2012-01-20 | 2016-10-25 | Kennesaw State University Research And Services Foundation, Inc. | Methods to mutate circular deoxyribonucleotides |
| US10760065B2 (en) * | 2013-09-05 | 2020-09-01 | Massachusetts Institute Of Technology | Tuning microbial populations with programmable nucleases |
| WO2015040075A1 (en) * | 2013-09-18 | 2015-03-26 | Genome Research Limited | Genomic screening methods using rna-guided endonucleases |
| WO2015071474A2 (en) * | 2013-11-18 | 2015-05-21 | Crispr Therapeutics Ag | Crispr-cas system materials and methods |
| US10287590B2 (en) * | 2014-02-12 | 2019-05-14 | Dna2.0, Inc. | Methods for generating libraries with co-varying regions of polynuleotides for genome modification |
| CN103911376B (zh) | 2014-04-03 | 2017-02-15 | 黄行许 | CRISPR‑Cas9靶向敲除乙肝病毒cccDNA及其特异性sgRNA |
| US10513711B2 (en) * | 2014-08-13 | 2019-12-24 | Dupont Us Holding, Llc | Genetic targeting in non-conventional yeast using an RNA-guided endonuclease |
| TWI716367B (zh) * | 2014-10-31 | 2021-01-21 | 麻省理工學院 | 用於常間回文重複序列叢集（ｃｒｉｓｐｒ）之大量平行組合性基因學 |
| WO2016130697A1 (en) * | 2015-02-11 | 2016-08-18 | Memorial Sloan Kettering Cancer Center | Methods and kits for generating vectors that co-express multiple target molecules |
-
2017
- 2017-12-27 EP EP17832242.6A patent/EP3562941B1/en active Active
- 2017-12-27 WO PCT/EP2017/084625 patent/WO2018122248A1/en unknown
- 2017-12-27 US US16/472,645 patent/US12091656B2/en active Active
- 2017-12-27 CN CN201780081590.7A patent/CN110249049A/zh active Pending
- 2017-12-27 JP JP2019535386A patent/JP7142637B2/ja active Active
- 2017-12-27 AU AU2017385611A patent/AU2017385611B2/en active Active
- 2017-12-27 CA CA3048939A patent/CA3048939A1/en active Pending
- 2017-12-27 ES ES17832242T patent/ES2940619T3/es active Active
- 2017-12-27 DK DK17832242.6T patent/DK3562941T5/da active
Also Published As
| Publication number | Publication date |
|---|---|
| US12091656B2 (en) | 2024-09-17 |
| CA3048939A1 (en) | 2018-07-05 |
| AU2017385611B2 (en) | 2023-11-02 |
| WO2018122248A1 (en) | 2018-07-05 |
| AU2017385611A1 (en) | 2019-07-18 |
| ES2940619T3 (es) | 2023-05-09 |
| JP7142637B2 (ja) | 2022-09-27 |
| AU2017385611A2 (en) | 2019-08-01 |
| EP3562941A1 (en) | 2019-11-06 |
| JP2020503044A (ja) | 2020-01-30 |
| US20190330616A1 (en) | 2019-10-31 |
| CN110249049A (zh) | 2019-09-17 |
| DK3562941T5 (da) | 2024-09-02 |
| EP3562941B1 (en) | 2022-12-21 |
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