DK3469076T3 - Mutant lipase og anvendelse deraf - Google Patents

Mutant lipase og anvendelse deraf Download PDF

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Publication number
DK3469076T3
DK3469076T3 DK17730432.6T DK17730432T DK3469076T3 DK 3469076 T3 DK3469076 T3 DK 3469076T3 DK 17730432 T DK17730432 T DK 17730432T DK 3469076 T3 DK3469076 T3 DK 3469076T3
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DK
Denmark
Prior art keywords
mutant lipase
lipase
mutant
Prior art date
Application number
DK17730432.6T
Other languages
English (en)
Inventor
Jong René Marcel De
Willem Bijleveld
Rij Evert Tjeerd Van
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of DK3469076T3 publication Critical patent/DK3469076T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C13/00—Cream; Cream preparations; Making thereof
    • A23C13/12—Cream preparations
    • A23C13/16—Cream preparations containing, or treated with, microorganisms, enzymes, or antibiotics; Sour cream
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C15/00—Butter; Butter preparations; Making thereof
    • A23C15/12—Butter preparations
    • A23C15/123—Addition of microorganisms or cultured milk products; Addition of enzymes; Addition of starter cultures other than destillates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/063—Addition of, or treatment with, enzymes or cell-free extracts of microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/068—Particular types of cheese
    • A23C19/08—Process cheese preparations; Making thereof, e.g. melting, emulsifying, sterilizing
    • A23C19/082—Adding substances to the curd before or during melting; Melting salts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
DK17730432.6T 2016-06-10 2017-06-08 Mutant lipase og anvendelse deraf DK3469076T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP16173955 2016-06-10
PCT/EP2017/063919 WO2017211930A1 (en) 2016-06-10 2017-06-08 Mutant lipase and use thereof

Publications (1)

Publication Number Publication Date
DK3469076T3 true DK3469076T3 (da) 2020-11-16

Family

ID=56132790

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17730432.6T DK3469076T3 (da) 2016-06-10 2017-06-08 Mutant lipase og anvendelse deraf

Country Status (5)

Country Link
US (1) US10829748B2 (da)
EP (1) EP3469076B1 (da)
CA (1) CA3025596A1 (da)
DK (1) DK3469076T3 (da)
WO (1) WO2017211930A1 (da)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3397771A2 (en) * 2015-12-30 2018-11-07 DSM IP Assets B.V. Partial enzymatic hydrolysis of triacylglycerols
EP3469076B1 (en) * 2016-06-10 2020-09-02 DSM IP Assets B.V. Mutant lipase and use thereof
CN116134147A (zh) 2020-07-27 2023-05-16 帝斯曼知识产权资产管理有限公司 脂肪酸视黄酯形成的减少
JPWO2022168954A1 (da) 2021-02-04 2022-08-11
US20230190888A1 (en) * 2021-12-16 2023-06-22 First Wave BioPharma, Inc. Stable lipase formulations and methods thereof

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2679920A1 (fr) 1991-08-02 1993-02-05 Rhone Poulenc Rorer Sa Levures recombinantes hautement stables pour la production de proteines recombinantes, leur preparation et leur utilisation.
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
EP1012301A1 (en) 1997-09-16 2000-06-28 Unilever N.V. TOTAL SYNTHESIS AND FUNCTIONAL OVEREXPRESSION OF A $i(CANDIDA RUGOSA) LIP1 GENE CODING FOR A MAJOR INDUSTRIAL LIPASE
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
EP1130100A1 (en) 2000-02-14 2001-09-05 Unilever N.V. Modified lipolytic enzymes and their use
FR2826470B1 (fr) 2001-06-26 2003-09-19 Astrium Sas Procede et dispositif de pilotage de l'attitude et de guidage d'un satellite par grappe de gyrodynes
US7402383B2 (en) 2001-07-23 2008-07-22 Dsm Ip Assets B.V. Process for preparing variant polynucleotides
WO2005059149A2 (en) 2003-12-12 2005-06-30 Chromagenics B.V. Improved protein production
US20060188625A1 (en) 2004-07-12 2006-08-24 Jan Gerrit Kortes Method to improve taste of food or beverage with a reduced amount of total fat by addition of yeast extract and food or beverage thereof
PL2410048T3 (pl) 2005-01-24 2017-02-28 Dsm Ip Assets B.V. Sposób otrzymywania związku będącego przedmiotem zainteresowania w komórce grzyba nitkowatego
BRPI0713795B1 (pt) 2006-06-29 2018-03-20 Dsm Ip Assets B.V. Método de otimização de uma sequência de nucleotídeos codificadora que codifica uma sequência de aminoácidos predeterminada
MX2009012926A (es) 2007-07-10 2010-01-14 Dsm Ip Assets Bv Autolisados de levadura.
US20120041171A1 (en) 2009-04-22 2012-02-16 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
US8828462B2 (en) 2011-05-12 2014-09-09 Griffith Laboratories International, Inc. Method for preparing a proteinaceous vegetable flavor enhancer
CN103582428B (zh) 2011-05-31 2016-10-26 帝斯曼知识产权资产管理有限公司 生产包含还原糖的酵母衍生物的方法
WO2013007821A1 (en) 2011-07-14 2013-01-17 Dsm Ip Assets B.V. Screening method
US10415023B2 (en) * 2013-12-10 2019-09-17 Amano Enzyme Inc. Modified lipase and use thereof
EP3469076B1 (en) * 2016-06-10 2020-09-02 DSM IP Assets B.V. Mutant lipase and use thereof

Also Published As

Publication number Publication date
EP3469076B1 (en) 2020-09-02
CA3025596A1 (en) 2017-12-14
US10829748B2 (en) 2020-11-10
EP3469076A1 (en) 2019-04-17
US20190185830A1 (en) 2019-06-20
WO2017211930A1 (en) 2017-12-14

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