DK3392336T3 - Effektiv mutant af phospholipase C, der ikke er afhængig af zink-ioner - Google Patents

Effektiv mutant af phospholipase C, der ikke er afhængig af zink-ioner Download PDF

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Publication number
DK3392336T3
DK3392336T3 DK16874858.0T DK16874858T DK3392336T3 DK 3392336 T3 DK3392336 T3 DK 3392336T3 DK 16874858 T DK16874858 T DK 16874858T DK 3392336 T3 DK3392336 T3 DK 3392336T3
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Denmark
Prior art keywords
phospholipase
dependent
zinc ions
effective mutant
mutant
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Application number
DK16874858.0T
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English (en)
Inventor
Yaoji Xuan
Sitian Gu
Wei Wu
Sha Liu
Yueyi Bao
Qiwen Niu
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Wilmar Shanghai Biotechnology Res & Dev Ct Co Ltd
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Publication of DK3392336T3 publication Critical patent/DK3392336T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/04—Phosphoric diester hydrolases (3.1.4)
    • C12Y301/04003—Phospholipase C (3.1.4.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/085—Bacillus cereus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK16874858.0T 2015-12-16 2016-12-15 Effektiv mutant af phospholipase C, der ikke er afhængig af zink-ioner DK3392336T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201510946696.1A CN106884009A (zh) 2015-12-16 2015-12-16 高效的不依赖锌离子的磷脂酶c突变体
PCT/CN2016/110030 WO2017101801A1 (zh) 2015-12-16 2016-12-15 高效的不依赖锌离子的磷脂酶c突变体

Publications (1)

Publication Number Publication Date
DK3392336T3 true DK3392336T3 (da) 2021-07-12

Family

ID=59055726

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16874858.0T DK3392336T3 (da) 2015-12-16 2016-12-15 Effektiv mutant af phospholipase C, der ikke er afhængig af zink-ioner

Country Status (6)

Country Link
US (1) US10738288B2 (da)
EP (1) EP3392336B1 (da)
CN (2) CN106884009A (da)
DK (1) DK3392336T3 (da)
ES (1) ES2882357T3 (da)
WO (1) WO2017101801A1 (da)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109321546B (zh) * 2017-12-12 2021-08-31 丰益(上海)生物技术研发中心有限公司 磷脂酶c及其编码基因
CN108384768A (zh) * 2018-02-28 2018-08-10 江苏中酶生物科技有限公司 一种磷脂酶c的突变体及其应用
CN111378585B (zh) * 2018-12-28 2023-06-16 丰益(上海)生物技术研发中心有限公司 用于表达外源基因的毕赤酵母突变株
CN111378634B (zh) * 2018-12-28 2024-04-02 丰益(上海)生物技术研发中心有限公司 具有磷脂酶c活性的多肽及其用途
CN111378633B (zh) * 2018-12-28 2024-03-29 丰益(上海)生物技术研发中心有限公司 一种高酶活的磷脂酶c突变体
CN111269905B (zh) * 2020-03-26 2023-09-01 广东燕京啤酒有限公司 用于降低啤酒非生物性浑浊的热稳定性β-1,3-1,4-葡聚糖酶
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN118272341A (zh) * 2022-12-21 2024-07-02 丰益(上海)生物技术研发中心有限公司 突变的脂肪酶

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
WO2015017045A1 (en) * 2013-07-03 2015-02-05 Keclon Sa Modified bacillus cereus phospholipase c protein and method of processing vegetable oil
CN104630174B (zh) 2013-11-07 2019-09-27 丰益(上海)生物技术研发中心有限公司 磷脂酶c突变体及其用途

Also Published As

Publication number Publication date
ES2882357T3 (es) 2021-12-01
WO2017101801A1 (zh) 2017-06-22
US10738288B2 (en) 2020-08-11
EP3392336A1 (en) 2018-10-24
CN106884009A (zh) 2017-06-23
US20180362942A1 (en) 2018-12-20
EP3392336A4 (en) 2019-05-15
EP3392336B1 (en) 2021-06-23
CN108431218A (zh) 2018-08-21
CN108431218B (zh) 2020-06-02

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