DK3307882T3 - Mutated photobacterium transsialidases - Google Patents

Mutated photobacterium transsialidases Download PDF

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Publication number
DK3307882T3
DK3307882T3 DK16806993.8T DK16806993T DK3307882T3 DK 3307882 T3 DK3307882 T3 DK 3307882T3 DK 16806993 T DK16806993 T DK 16806993T DK 3307882 T3 DK3307882 T3 DK 3307882T3
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DK
Denmark
Prior art keywords
transsialidases
photobacterium
mutated
photobacterium transsialidases
mutated photobacterium
Prior art date
Application number
DK16806993.8T
Other languages
English (en)
Inventor
Andreas Vogel
Ramona Schmiedel
Elise Champion
Gyula Dekany
Original Assignee
Glycom As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycom As filed Critical Glycom As
Application granted granted Critical
Publication of DK3307882T3 publication Critical patent/DK3307882T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/005Glycopeptides, glycoproteins

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK16806993.8T 2015-06-09 2016-06-09 Mutated photobacterium transsialidases DK3307882T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15171175 2015-06-09
PCT/IB2016/053410 WO2016199069A1 (en) 2015-06-09 2016-06-09 Mutated sialidases

Publications (1)

Publication Number Publication Date
DK3307882T3 true DK3307882T3 (da) 2020-05-18

Family

ID=53365908

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16806993.8T DK3307882T3 (da) 2015-06-09 2016-06-09 Mutated photobacterium transsialidases

Country Status (5)

Country Link
US (1) US10428318B2 (da)
EP (1) EP3307882B1 (da)
DK (1) DK3307882T3 (da)
ES (1) ES2798373T3 (da)
WO (1) WO2016199069A1 (da)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3645545A4 (en) 2017-06-30 2021-05-05 Glycom A/S PURIFICATION OF OLIGOSACCHARIDES
CN111133112A (zh) * 2017-07-26 2020-05-08 詹尼温生物技术有限责任公司 唾液酸转移酶及其在生产唾液酸化低聚糖中的用途
MX2020007024A (es) 2018-01-03 2020-10-28 Palleon Pharmaceuticals Inc Sialidasas humanas recombinantes, proteinas de fusion de sialidasa y metodos para usar las mismas.

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827714A (en) 1994-12-07 1998-10-27 Japan Tobacco Inc. β-galactoside-α-2, 6-sialyltransferase, and a process for producing from Photobacterium
JP3140976B2 (ja) 1997-02-28 2001-03-05 日本たばこ産業株式会社 β−ガラクトシド−α2,6−シアル酸転移酵素をコードする遺伝子
WO2007105305A1 (ja) 2006-03-14 2007-09-20 Japan Tobacco Inc. 新規なβ-ガラクトシド-α2,6-シアル酸転移酵素、それをコードする遺伝子およびその製造方法
EP2116598A4 (en) 2007-03-02 2010-06-09 Japan Tobacco Inc Novel Beta GALACTOSIDE ALPHA-2,6-SIALYLTRANSFERASE, GENE FOR THEIR CODING AND METHOD FOR INCREASING ENZYMATIC ACTIVITY
JPWO2010143713A1 (ja) 2009-06-12 2012-11-29 日本たばこ産業株式会社 新規タンパク質およびそれをコードする遺伝子
KR20140001198A (ko) * 2010-07-16 2014-01-06 글리콤 에이/에스 신규 시알로올리고당 유도체들의 합성
JP6106160B2 (ja) * 2011-05-13 2017-03-29 グリコム アー/エスGlycom A/S 人乳オリゴ糖(hmo)又はその前駆体の多様化
KR101574952B1 (ko) 2013-04-11 2015-12-07 서울대학교 산학협력단 α-2,3 및 α-2,6 시알산 전이효소의 변이체 탐색 방법 및 시알릴올리고당 생산에의 응용
CN105246911B (zh) 2013-04-12 2019-10-01 丹麦科技大学 用于产生唾液酸化聚糖之具有转唾液酸酶活性的突变体唾液酸酶
KR101755430B1 (ko) 2014-09-25 2017-07-27 한국생명공학연구원 개선된 효율을 갖는 시알산전달효소를 이용한 당단백질의 당사슬에 시알산을 부가하는 방법

Also Published As

Publication number Publication date
EP3307882A4 (en) 2019-01-09
EP3307882A1 (en) 2018-04-18
EP3307882B1 (en) 2020-04-15
US10428318B2 (en) 2019-10-01
US20180163185A1 (en) 2018-06-14
ES2798373T3 (es) 2020-12-11
WO2016199069A1 (en) 2016-12-15

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