DK3234146T3 - Selektiv optimering af et ribosombindingssted til proteinproduktion - Google Patents
Selektiv optimering af et ribosombindingssted til proteinproduktion Download PDFInfo
- Publication number
- DK3234146T3 DK3234146T3 DK15823835.2T DK15823835T DK3234146T3 DK 3234146 T3 DK3234146 T3 DK 3234146T3 DK 15823835 T DK15823835 T DK 15823835T DK 3234146 T3 DK3234146 T3 DK 3234146T3
- Authority
- DK
- Denmark
- Prior art keywords
- protein
- ribos
- bonding
- selective optimization
- production
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE1451553 | 2014-12-16 | ||
| PCT/SE2015/051343 WO2016099388A1 (en) | 2014-12-16 | 2015-12-15 | Selective optimisation of a ribosome binding site for protein production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3234146T3 true DK3234146T3 (da) | 2019-08-05 |
Family
ID=55135500
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK15823835.2T DK3234146T3 (da) | 2014-12-16 | 2015-12-15 | Selektiv optimering af et ribosombindingssted til proteinproduktion |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US10696963B2 (da) |
| EP (1) | EP3234146B1 (da) |
| DK (1) | DK3234146T3 (da) |
| WO (1) | WO2016099388A1 (da) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11458172B2 (en) * | 2017-09-08 | 2022-10-04 | New Portal Limited | Nucleic acid systems that enable bacteria to specifically target solid tumors via glucose-dependent viability |
| EP3480318A1 (en) * | 2017-11-06 | 2019-05-08 | Roquette Freres | A genetically modified bacillus subtilis strain, optimized vectors, and uses thereof |
| WO2020194311A1 (en) * | 2019-03-28 | 2020-10-01 | Ramot At Tel-Aviv University Ltd. | Methods for modifying translation |
| SE544059C2 (en) * | 2020-02-07 | 2021-11-30 | Cloneopt Ab | Synthetically evolved dna constructs for regulating signal peptide performance as well as vectors and host cells thereof |
| EP4100534A4 (en) * | 2020-02-07 | 2024-06-05 | Xbrane Biopharma Ab | SYNTHETIC DNA CONSTRUCTS FOR REGULATING SIGNAL PEPTIDE PERFORMANCE AND VECTORS, HOST CELLS AND RECOMBINANT PROTEINS THEREOF |
| SE543946C2 (en) * | 2020-02-07 | 2021-10-05 | Cloneopt Ab | Synthetically evolved dna constructs for regulating signal peptide performance as well as vectors and host cells thereof |
| SE543945C2 (en) * | 2020-02-07 | 2021-10-05 | Cloneopt Ab | Synthetically evolved DNA constructs for regulating signal peptide performance as well as vectors and host cells thereof |
| JP7434125B2 (ja) * | 2020-09-16 | 2024-02-20 | 株式会社東芝 | 文書検索装置、文書検索方法、及びプログラム |
| AU2022349814A1 (en) | 2021-09-24 | 2024-05-02 | Xbrane Biopharma Ab | Dna constructs and host cells for expressing recombinant protein |
| CN115960934A (zh) * | 2022-08-24 | 2023-04-14 | 深圳柏垠生物科技有限公司 | 大肠杆菌表达外源基因优化方法及其序列 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7258995B2 (en) * | 2001-01-12 | 2007-08-21 | Trustees Of Dartmouth College | Compositions and methods for affecting virulence determinants in bacteria |
| AU2009224600A1 (en) | 2008-03-14 | 2009-09-17 | Merck Serono S.A. | Variation of recombinant expression titres by optimising bacterial ribosome binding sites |
-
2015
- 2015-12-15 WO PCT/SE2015/051343 patent/WO2016099388A1/en active Application Filing
- 2015-12-15 US US15/535,686 patent/US10696963B2/en active Active
- 2015-12-15 EP EP15823835.2A patent/EP3234146B1/en active Active
- 2015-12-15 DK DK15823835.2T patent/DK3234146T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3234146A1 (en) | 2017-10-25 |
| US20180273934A1 (en) | 2018-09-27 |
| US10696963B2 (en) | 2020-06-30 |
| WO2016099388A1 (en) | 2016-06-23 |
| EP3234146B1 (en) | 2019-07-10 |
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