DK3149153T3 - Forøgelse af lipidproduktion i olieholdig gær - Google Patents

Forøgelse af lipidproduktion i olieholdig gær Download PDF

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Publication number
DK3149153T3
DK3149153T3 DK15800396.2T DK15800396T DK3149153T3 DK 3149153 T3 DK3149153 T3 DK 3149153T3 DK 15800396 T DK15800396 T DK 15800396T DK 3149153 T3 DK3149153 T3 DK 3149153T3
Authority
DK
Denmark
Prior art keywords
enhancement
lipid production
oily yeast
oily
yeast
Prior art date
Application number
DK15800396.2T
Other languages
English (en)
Inventor
Elena E Brevnova
Vasiliki Tsakraklides
Original Assignee
Ginkgo Bioworks Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ginkgo Bioworks Inc filed Critical Ginkgo Bioworks Inc
Application granted granted Critical
Publication of DK3149153T3 publication Critical patent/DK3149153T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK15800396.2T 2014-05-29 2015-05-29 Forøgelse af lipidproduktion i olieholdig gær DK3149153T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462004502P 2014-05-29 2014-05-29
US201462033853P 2014-08-06 2014-08-06
US201462090169P 2014-12-10 2014-12-10
PCT/US2015/033251 WO2015184303A1 (en) 2014-05-29 2015-05-29 Increasing lipid production in oleaginous yeast

Publications (1)

Publication Number Publication Date
DK3149153T3 true DK3149153T3 (da) 2022-10-03

Family

ID=54699886

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15800396.2T DK3149153T3 (da) 2014-05-29 2015-05-29 Forøgelse af lipidproduktion i olieholdig gær

Country Status (6)

Country Link
US (2) US10260077B2 (da)
EP (2) EP3149153B1 (da)
CN (1) CN106715678A (da)
AU (2) AU2015266724B2 (da)
DK (1) DK3149153T3 (da)
WO (1) WO2015184303A1 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015252916B2 (en) * 2014-05-01 2018-08-09 Ginkgo Bioworks, Inc. Increasing cellular lipid production by increasing the activity of diacylglycerol acyltransferase and decreasing the activity of triacylglycerol lipase
EP3149153B1 (en) 2014-05-29 2022-07-13 Ginkgo Bioworks, Inc. Increasing lipid production in oleaginous yeast
WO2017123913A1 (en) * 2016-01-14 2017-07-20 Novogy, Inc. Engineered lubricant
CN106701606B (zh) * 2016-12-19 2021-03-26 广东利世康低碳科技有限公司 一种能降解利用餐厨废弃物的基因工程产朊假丝酵母及其构建方法
CN115838644A (zh) * 2017-10-13 2023-03-24 武汉合生科技有限公司 微生物及提高微生物疏水化合物发酵产量的方法
CN110713942B (zh) * 2019-11-28 2022-07-08 安徽师范大学 一种耐铜锌的盐酸土霉素降解菌及其发酵液和应用
CN112522126B (zh) * 2020-12-29 2022-07-22 南京理工大学 高产微生物油脂红酵母基因工程菌及其构建和培养方法
WO2023122805A1 (en) * 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
CN116555362A (zh) * 2022-01-29 2023-08-08 罗盖特公司 使用解脂耶氏酵母生产脂质的方法

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* Cited by examiner, † Cited by third party
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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US20030170691A1 (en) 2001-12-19 2003-09-11 Millennium Pharmaceuticals, Inc. Human diacylglycerol acyltransferase 2 (DGAT2) family members and uses therefor
US7314974B2 (en) 2002-02-21 2008-01-01 Monsanto Technology, Llc Expression of microbial proteins in plants for production of plants with improved properties
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7198937B2 (en) 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7588931B2 (en) 2004-11-04 2009-09-15 E. I. Du Pont De Nemours And Company High arachidonic acid producing strains of Yarrowia lipolytica
US7192762B2 (en) 2004-11-04 2007-03-20 E. I. Du Pont De Nemours And Company Mortierella alpina glycerol-3-phosphate o-acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
EP2078092A2 (en) * 2006-09-28 2009-07-15 Microbia, Inc. Production of carotenoids in oleaginous yeast and fungi
WO2008130372A2 (en) 2006-09-28 2008-10-30 Microbia, Inc. Production of sterols in oleaginous yeast and fungi
MX2009012596A (es) 2007-05-24 2009-12-08 Du Pont Genes de diacilglicerol aciltransferasa de yarrowia lipolytica para la produccion mejorada de lipidos de almacenamiento de semillas y para los perfiles alterados de acidos grasos en el frijol de soya.
CA2722275A1 (en) * 2008-04-25 2009-10-29 Commonwealth Scientific And Industrial Research Organisation Polypeptides and methods for producing triacylglycerols comprising modified fatty acids
US20090291479A1 (en) * 2008-05-23 2009-11-26 E. I. Du Pont De Nemours And Company Manipulation of acyl-coa binding protein expression for altered lipid production in microbial hosts
EP2620501A3 (en) 2008-05-23 2013-10-02 E. I. du Pont de Nemours and Company DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
WO2010025374A2 (en) 2008-08-29 2010-03-04 E. I. Du Pont De Nemours And Company Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms
US8502026B2 (en) 2009-08-24 2013-08-06 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
EP2292741A1 (en) 2009-08-26 2011-03-09 OrganoBalance GmbH Genetically modified organisms for the production of lipids
WO2011127118A1 (en) * 2010-04-06 2011-10-13 Algenetix, Inc. Methods of producing oil in non-plant organisms
FR2962133B1 (fr) 2010-07-01 2014-09-12 Agronomique Inst Nat Rech Optimisation de la synthese et de l'accumulation de lipides
RU2652875C2 (ru) 2011-10-19 2018-05-03 Массачусетс Инститьют Оф Текнолоджи Сконструированные микробы и способы получения микробного масла
WO2014062163A1 (en) 2012-10-16 2014-04-24 Exxonmobil Research And Engineering Company Dgat genes and methods of use for triglyceride production in recombinant microorganisms
FR3002774A1 (fr) * 2013-03-04 2014-09-05 Agronomique Inst Nat Rech Levures mutantes ayant une production accrue de lipides et d'acide citrique
WO2014182657A1 (en) 2013-05-06 2014-11-13 Novogy, Inc. Increasing homologous recombination during cell transformation
EP3110947B1 (en) 2014-02-24 2019-09-04 Novogy Inc. Diacylglycerol acyl transferase (dga1) polynucleotides, and methods of increasing yeast cell lipid production by overexpression of heterologous dga1
AU2015252916B2 (en) * 2014-05-01 2018-08-09 Ginkgo Bioworks, Inc. Increasing cellular lipid production by increasing the activity of diacylglycerol acyltransferase and decreasing the activity of triacylglycerol lipase
CN108064268A (zh) * 2014-05-29 2018-05-22 诺沃吉公司 增加脂质产生和优化脂质组成
EP3149153B1 (en) 2014-05-29 2022-07-13 Ginkgo Bioworks, Inc. Increasing lipid production in oleaginous yeast
CN107208088B (zh) 2014-12-10 2022-02-11 银杏生物制品公司 酵母中的油酸生产
WO2016109494A1 (en) 2014-12-30 2016-07-07 Novogy, Inc. Enhanced production of core lipids in oleaginous yeasts

Also Published As

Publication number Publication date
WO2015184303A1 (en) 2015-12-03
US10260077B2 (en) 2019-04-16
EP3149153B1 (en) 2022-07-13
US11492647B2 (en) 2022-11-08
EP3149153A4 (en) 2017-11-29
US20170191093A1 (en) 2017-07-06
AU2015266724A1 (en) 2016-12-15
AU2021225189A1 (en) 2021-09-30
US20190225996A1 (en) 2019-07-25
EP4174186A1 (en) 2023-05-03
AU2015266724B2 (en) 2021-09-23
CN106715678A (zh) 2017-05-24
EP3149153A1 (en) 2017-04-05

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