DK3036326T3 - INHIBITION OF A LNCRNA FOR TREATMENT OF MELANOMES - Google Patents
INHIBITION OF A LNCRNA FOR TREATMENT OF MELANOMES Download PDFInfo
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- DK3036326T3 DK3036326T3 DK14757881.9T DK14757881T DK3036326T3 DK 3036326 T3 DK3036326 T3 DK 3036326T3 DK 14757881 T DK14757881 T DK 14757881T DK 3036326 T3 DK3036326 T3 DK 3036326T3
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- linc01212
- melanoma
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| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11591601B2 (en) | 2017-05-05 | 2023-02-28 | The Broad Institute, Inc. | Methods for identification and modification of lncRNA associated with target genotypes and phenotypes |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| EP3658573A1 (en) | 2017-07-28 | 2020-06-03 | President and Fellows of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace) |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| AU2018352592C1 (en) | 2017-10-16 | 2025-09-25 | Beam Therapeutics, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019165212A1 (en) * | 2018-02-22 | 2019-08-29 | University Of Pittsburgh - Of The Commonwealth System Of Higher Education | TARGETING CANCER-ASSOCIATED LONG NON-CODING RNAs |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| GB2601617B (en) | 2019-03-19 | 2024-02-21 | Broad Inst Inc | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| EP3976055A1 (en) * | 2019-05-29 | 2022-04-06 | Universiteit Gent | Combinations of therapeutic agents for treating uveal melanoma |
| KR102238594B1 (ko) * | 2019-06-07 | 2021-04-08 | 한양대학교 산학협력단 | Heres 발현 억제제를 포함하는 편평상피세포암 예방 또는 치료용 약학적 조성물 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN110898213A (zh) * | 2019-11-27 | 2020-03-24 | 西安交通大学 | LncRNA SAMMSON与BRAF激酶联合抑制在延缓黑色素瘤适应性耐药的应用 |
| CA3161513A1 (en) * | 2020-01-28 | 2021-08-05 | Irwin DAVIDSON | Antisense oligonucleotide targeting linc00518 for treating melanoma |
| DE112021002672T5 (de) | 2020-05-08 | 2023-04-13 | President And Fellows Of Harvard College | Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz |
| CN112553208B (zh) * | 2020-12-31 | 2023-09-26 | 重庆市畜牧科学院 | 一个长链非编码rna新基因及其在制备检测或诊断早期黑变病试剂中的应用 |
| CN112760377B (zh) * | 2021-01-18 | 2023-11-03 | 南通大学附属医院 | lncRNA 068在诊断或治疗恶性黑色素瘤方面的应用 |
| WO2023086552A2 (en) * | 2021-11-12 | 2023-05-19 | The Regents Of The University Of California | Lncrna transcripts in melanomagenesis |
| EP4299742A1 (en) | 2022-06-30 | 2024-01-03 | Université de Strasbourg | Combination therapy for melanoma |
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| EP1929043A4 (en) * | 2005-08-24 | 2009-10-28 | Genizon Biosciences Inc | GENETIC CARD OF HUMAN GENES ASSOCIATED WITH CROHN'S DISEASE |
| CN101633923B (zh) * | 2009-07-10 | 2011-04-13 | 四川大学 | 人黑色素瘤细胞相关的长非编码rna及其用途 |
| WO2012018881A2 (en) * | 2010-08-03 | 2012-02-09 | Alnylam Pharmaceuticals, Inc. | Methods and compositions for the regulation of rna |
| US9410206B2 (en) * | 2011-11-30 | 2016-08-09 | John Wayne Cancer Institute | Long noncoding RNA (lncRNA) as a biomarker and therapeutic marker in cancer |
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