DK2890498T3 - Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede celler eller cellestrukturer - Google Patents
Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede celler eller cellestrukturer Download PDFInfo
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- DK2890498T3 DK2890498T3 DK13832402.5T DK13832402T DK2890498T3 DK 2890498 T3 DK2890498 T3 DK 2890498T3 DK 13832402 T DK13832402 T DK 13832402T DK 2890498 T3 DK2890498 T3 DK 2890498T3
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- sperm
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- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
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- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
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- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
Landscapes
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Soft Magnetic Materials (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (3)
1. Fremgangsmåde til magnetisk cellemanipulering, hvilken fremgangsmåde omfatter: etablering af kontakt mellem en sammensætning og en biologisk prøve for at danne en blanding, hvilken sammensætning omfatter en flerhed af partikler, hvor hver partikel i flerheden af partikler omfatter: et magnetisk substrat, hvilket magnetiske substrat er kendetegnet ved en magnetisk susceptibilitet, som er større end nul; og en opladelig siliciumholdig forbindelse, som omfatter 2-(carbomethoxy)ethyltrimethoxysilan, hvilken opladelige siliciumholdige forbindelse dækker mindst en del af det magnetiske substrat; og hvilken biologisk prøve omfatter levedygtige sædceller og beskadigede eller kompromitterede sædceller, hvilke partikler danner komplekser med de beskadigede eller kompromitterede sædceller, og hvor den opladelige siliciumholdige forbindelse omfatter en første zetapotential-ladning, hvilken første zetapotential-ladning har modsat fortegn i sammenligning med en anden zetapotential-ladning, som er omfattet af de beskadigede eller kompromitterede sædceller i den biologiske prøve; og påtrykning af et magnetfelt på blandingen for at manipulere sammensætningen; og separation af de levedygtige sædceller fra komplekserne.
2. Fremgangsmåde ifølge krav 1, som endvidere omfatter underkastelse af den biologiske prøve for sædcelledetektion.
3. Fremgangsmåde ifølge krav 2, hvor detektionen omfatter fluorescensdetektion.
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| US201261694756P | 2012-08-29 | 2012-08-29 | |
| PCT/US2013/056526 WO2014035840A1 (en) | 2012-08-29 | 2013-08-23 | Magnetic removal or identification of damaged or compromised cells or cellular structures |
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| DK19180225.5T DK3563933T3 (da) | 2012-08-29 | 2013-08-23 | Magnetisk fjernelse eller identificering af beskadigede eller svækkede celler eller cellestrukturer |
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