DK2890498T3 - Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede celler eller cellestrukturer - Google Patents
Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede celler eller cellestrukturer Download PDFInfo
- Publication number
- DK2890498T3 DK2890498T3 DK13832402.5T DK13832402T DK2890498T3 DK 2890498 T3 DK2890498 T3 DK 2890498T3 DK 13832402 T DK13832402 T DK 13832402T DK 2890498 T3 DK2890498 T3 DK 2890498T3
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- Denmark
- Prior art keywords
- sperm
- cells
- magnetic
- particles
- damaged
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/015—Pretreatment specially adapted for magnetic separation by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/023—Separation using Lorentz force, i.e. deflection of electrically charged particles in a magnetic field
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/288—Magnetic plugs and dipsticks disposed at the outer circumference of a recipient
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Soft Magnetic Materials (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Claims (3)
1. Fremgangsmåde til magnetisk cellemanipulering, hvilken fremgangsmåde omfatter: etablering af kontakt mellem en sammensætning og en biologisk prøve for at danne en blanding, hvilken sammensætning omfatter en flerhed af partikler, hvor hver partikel i flerheden af partikler omfatter: et magnetisk substrat, hvilket magnetiske substrat er kendetegnet ved en magnetisk susceptibilitet, som er større end nul; og en opladelig siliciumholdig forbindelse, som omfatter 2-(carbomethoxy)ethyltrimethoxysilan, hvilken opladelige siliciumholdige forbindelse dækker mindst en del af det magnetiske substrat; og hvilken biologisk prøve omfatter levedygtige sædceller og beskadigede eller kompromitterede sædceller, hvilke partikler danner komplekser med de beskadigede eller kompromitterede sædceller, og hvor den opladelige siliciumholdige forbindelse omfatter en første zetapotential-ladning, hvilken første zetapotential-ladning har modsat fortegn i sammenligning med en anden zetapotential-ladning, som er omfattet af de beskadigede eller kompromitterede sædceller i den biologiske prøve; og påtrykning af et magnetfelt på blandingen for at manipulere sammensætningen; og separation af de levedygtige sædceller fra komplekserne.
2. Fremgangsmåde ifølge krav 1, som endvidere omfatter underkastelse af den biologiske prøve for sædcelledetektion.
3. Fremgangsmåde ifølge krav 2, hvor detektionen omfatter fluorescensdetektion.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261694756P | 2012-08-29 | 2012-08-29 | |
PCT/US2013/056526 WO2014035840A1 (en) | 2012-08-29 | 2013-08-23 | Magnetic removal or identification of damaged or compromised cells or cellular structures |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2890498T3 true DK2890498T3 (da) | 2018-05-22 |
Family
ID=50184192
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK13832402.5T DK2890498T3 (da) | 2012-08-29 | 2013-08-23 | Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede celler eller cellestrukturer |
DK19180225.5T DK3563933T3 (da) | 2012-08-29 | 2013-08-23 | Magnetisk fjernelse eller identificering af beskadigede eller svækkede celler eller cellestrukturer |
DK17208185T DK3326721T3 (da) | 2012-08-29 | 2013-08-23 | Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede spermatozoer |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK19180225.5T DK3563933T3 (da) | 2012-08-29 | 2013-08-23 | Magnetisk fjernelse eller identificering af beskadigede eller svækkede celler eller cellestrukturer |
DK17208185T DK3326721T3 (da) | 2012-08-29 | 2013-08-23 | Magnetisk fjernelse eller identificering af beskadigede eller kompromitterede spermatozoer |
Country Status (12)
Country | Link |
---|---|
US (5) | US9804153B2 (da) |
EP (4) | EP3326721B1 (da) |
JP (1) | JP2015528293A (da) |
AU (6) | AU2013309137B2 (da) |
BR (1) | BR112015004374B1 (da) |
CA (1) | CA2883328C (da) |
DK (3) | DK2890498T3 (da) |
ES (3) | ES2757884T3 (da) |
IN (1) | IN2015KN00538A (da) |
NZ (2) | NZ705665A (da) |
RU (1) | RU2015111211A (da) |
WO (1) | WO2014035840A1 (da) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
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US10379026B2 (en) | 2012-08-29 | 2019-08-13 | Inguran, Llc | Cell processing using magnetic particles |
EP3326721B1 (en) * | 2012-08-29 | 2019-08-21 | Inguran, LLC | Magnetic removal or identification of damaged or compromised sperm cells |
KR101760467B1 (ko) | 2014-12-01 | 2017-07-24 | 중앙대학교 산학협력단 | 수태능력 관련 단백질 마커를 이용한 동물의 산자수 예측 방법과 클로르테트라사이클린 염색법을 이용한 동물의 정액 품질 및 산자수의 예측 방법 |
EP3227661A4 (en) * | 2014-12-05 | 2019-01-09 | Inguran, LLC | TREATMENT OF CELLS USING MAGNETIC PARTICLES |
JP5891320B1 (ja) * | 2015-02-12 | 2016-03-22 | 秋田県 | ゼータ電位制御法を用いた処理方法 |
US10030241B2 (en) | 2015-03-30 | 2018-07-24 | General Electric Company | Methods and kits for capturing sperm nucleic acids |
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US20210230541A1 (en) * | 2017-11-10 | 2021-07-29 | Cytolutions, Llc | Sexed Sperm Bulk Separation Systems |
WO2020032906A1 (en) * | 2018-08-10 | 2020-02-13 | T.C. Istanbul Medipol Universitesi | A method for use in determination of apoptotic sperms |
US11629330B2 (en) | 2019-03-19 | 2023-04-18 | Inguran, Llc | Method for improved sperm cell populations |
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US10379026B2 (en) * | 2012-08-29 | 2019-08-13 | Inguran, Llc | Cell processing using magnetic particles |
EP3326721B1 (en) * | 2012-08-29 | 2019-08-21 | Inguran, LLC | Magnetic removal or identification of damaged or compromised sperm cells |
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