DK2673072T3 - Celleseparationssystem med membran med pneumatisk vekslende tryk - Google Patents
Celleseparationssystem med membran med pneumatisk vekslende tryk Download PDFInfo
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- DK2673072T3 DK2673072T3 DK12703101.1T DK12703101T DK2673072T3 DK 2673072 T3 DK2673072 T3 DK 2673072T3 DK 12703101 T DK12703101 T DK 12703101T DK 2673072 T3 DK2673072 T3 DK 2673072T3
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- liquid
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/02—Hollow fibre modules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/20—Accessories; Auxiliary operations
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/22—Controlling or regulating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/16—Hollow fibers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/24—Specific pressurizing or depressurizing means
- B01D2313/243—Pumps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/50—Specific extra tanks
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/58—Parts of membrane modules specifically adapted for single use
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2315/00—Details relating to the membrane module operation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2321/00—Details relating to membrane cleaning, regeneration, sterilization or to the prevention of fouling
- B01D2321/04—Backflushing
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T29/00—Metal working
- Y10T29/49—Method of mechanical manufacture
- Y10T29/49826—Assembling or joining
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Water Supply & Treatment (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (18)
1. Væskefiltreringssystem, der omfatter: a) mindst én væskeopbevaringsbeholder; b) mindst ét filterindeholdende rum; c) mindst ét væskeoverføringsrør, der forbinder opbevaringsbeholderen med en indgangsende af et filterindeholdende rum, hvilket væskeoverføringsrør er i stand til at lede en væske fra opbevaringsbeholderen ind i indgangsenden af det filterindeholdende rum; d) mindst ét ekspansionskammer med mindst to åbninger, hvor en første åbning er forbundet til en udgangsende af det filterindeholdende rum, og hvor en anden åbning er forbundet til en gasstrømningsregulator; e) mindst én gasstrømningsregulator til skiftevis at forsyne ekspansionskammeret med positivt og negativt tryk; f) mindst én væskeudtagningsport, der er forbundet til det filterindeholdende rum, til fjernelse af filtreret væske fra det filterindeholdende rum, der er kendetegnet ved, at ekspansionskammeret ikke indeholder noget separeringsmiddel mellem den første og anden åbning; og hvor mindst én niveausensor er monteret på eller i ekspansionskammeret.
2. Væskefiltreringssystem ifølge krav 1, hvor ekspansionskammeret er forbundet til gasstrømningsregulatoren med en gasledning.
3. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-2, hvor gasledningen omfatter et filter mellem ekspansionskammeret og gasstrømningsregulatoren, fortrinsvis hvor filteret er et sterilt filter, og eventuelt hvor gasledningen omfatter et sterilt filter med varmelegeme.
4. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-3, hvor væskeopbevaringsbeholderen er en bioreaktor.
5. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-4, hvor det filterindeholdende rum indeholder et hulfiberfilter.
6. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-5, hvor mindst to niveausensorer er monteret på eller i ekspansionskammeret, og fortrinsvis hvor niveausensoren eller niveausensorerne er monteret på overfladen af ekspansionskammeret.
7. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-6, hvor niveausensorerne er i stand til at måle et minimalt og et maksimalt væskeniveau i ekspansionskammeret og er funktionelt koblet til gasstrømningsregulatoren.
8. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-7, der er i stand til at veksle mellem indsugning af væsken i systemet fra opbevaringsbeholderen gennem det filterindeholdende rum ind i ekspansionskammeret og udstødning af væsken fra ekspansionskammeret gennem det filterindeholdende rum tilbage i opbevaringsbeholderen, hvor væskeindsugning udføres ved påføring af negativt tryk i ekspansionskammeret, og væskeudstødning udføres ved påføring af positivt tryk i ekspansionskammeret.
9. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-8, hvor det negative tryk opnås ved frembringelse af et vakuum i ekspansionskammeret, og det positive tryk opnås ved injektion af gas, for eksempel komprimeret luft, i ekspansionskammeret.
10. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-9, hvor filteret i det filterindeholdende rum omfatter et antal bundtede hule fibre, hvis akser strækker sig på langs fra indgangsenden til udgangsenden af det filterindeholdende rum.
11. Væskefiltreringssystem ifølge et hvilket som helst af kravene 1-10, hvor det filterindeholdende rum og ekspansionskammeret er til engangsbrug, fortrinsvis hvor det filterindeholdende rum, ekspansionskammeret, væskeopbevaringsbeholderen og væskeoverføringsrøret er til engangsbrug.
12. Fremgangsmåde til filtrering af en væske, der omfatter: a) tilvejebringelse af et væskefiltreringssystem, der omfatter mindst én væskeopbevaringsbeholder; mindst ét filterindeholdende rum; et væskeoverføringsrør, der forbinder opbevaringsbeholderen med en indgangsende af det filterindeholdende rum, hvilket overføringsrør er i stand til at lede en væske fra opbevaringsbeholderen ind i indgangsenden af det filterindeholdende rum; mindst ét ekspansionskammer, der på den ene side er forbundet med en udgangsende af det filterindeholdende rum til muliggørelse af, at væske fra rummet kan løbe ind i kammeret, og på den anden side med en gasstrømningsregulator, der skiftevis tilvejebringer et negativt og positivt tryk i ekspansionskammeret til derved indsugning af væske fra udgangsenden af det filterindeholdende rum og udstødning af væske tilbage ind i udgangsenden af det filterindeholdende rum, hvilket frembringer en direkte gas-væske-grænseflade uden separeringsmiddel i ekspansionskammeret; og mindst én gennemtrængningsport, der er forbundet til det filterindeholdende rum, til fjernelse af filtreret væske fra det filterindeholdende rum; b) udtrækning af væske fra opbevaringsbeholderen gennem filteret ind i ekspansionskammeret ved påføring af negativt tryk i ekspansionskammeret; c) udstødning af væsken fra ekspansionskammeret gennem filteret tilbage ind i opbevaringsbeholderen ved påføring af positivt tryk i ekspansionskammeret; d) gentagelse af trin b og c til frembringelse af en alternativ tangential strømning af væske gennem filteret; og e) fjernelse af filtreret væske fra filtreringssystemet.
13. Fremgangsmåde til filtrering af en væske ifølge krav 12, hvor væskeopbevaringsbeholderen er en bioreaktor.
14. Fremgangsmåde til filtrering af en væske ifølge et hvilket som helst af kravene 12-13, hvor det filterindeholdende rum og/eller ekspansionskammeret er til engangsbrug, fortrinsvis hvor det filterindeholdende rum, ekspansionskammeret, væskeopbevaringsbeholderen og væskeoverføringsrøret er til engangsbrug.
15. Fremgangsmåde til filtrering af en væske ifølge et hvilket som helst af kravene 12-14, hvor det positive og negative tryk reguleres ved hjælp af niveausensorer, der måler væskeniveauet i ekspansionskammeret.
16. Fremgangsmåde til filtrering af en væske ifølge krav 15, hvor niveausensorerne er monteret på ekspansionskammeret.
17. Fremgangsmåde til filtrering af en væske ifølge et hvilket som helst af kravene 12-16, hvor det filterindeholdende rum indeholder et hulfiberfilter.
18. Fremgangsmåde til filtrering af en væske ifølge et hvilket som helst af kravene 12-17, hvor det negative tryk opnås ved frembringelse af et vakuum i ekspansionskammeret, og det positive tryk opnås ved injektion af gas, for eksempel komprimeret luft, i ekspansionskammeret.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161463095P | 2011-02-10 | 2011-02-10 | |
| EP11153995 | 2011-02-10 | ||
| EP11183676 | 2011-10-03 | ||
| US201161627812P | 2011-10-18 | 2011-10-18 | |
| PCT/EP2012/052031 WO2012107436A1 (en) | 2011-02-10 | 2012-02-07 | Pneumatic alternating pressure membrane cell separation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2673072T3 true DK2673072T3 (da) | 2015-02-16 |
Family
ID=45569654
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12703101.1T DK2673072T3 (da) | 2011-02-10 | 2012-02-07 | Celleseparationssystem med membran med pneumatisk vekslende tryk |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US8845902B2 (da) |
| EP (1) | EP2673072B1 (da) |
| JP (1) | JP5893051B2 (da) |
| KR (1) | KR101890000B1 (da) |
| CN (1) | CN103347595B (da) |
| AU (1) | AU2012215514B2 (da) |
| BR (1) | BR112013019325B1 (da) |
| CA (1) | CA2824907C (da) |
| DK (1) | DK2673072T3 (da) |
| EA (1) | EA023614B1 (da) |
| ES (1) | ES2529429T3 (da) |
| IL (1) | IL227833A (da) |
| MX (1) | MX344465B (da) |
| PL (1) | PL2673072T3 (da) |
| SG (1) | SG192611A1 (da) |
| WO (1) | WO2012107436A1 (da) |
| ZA (1) | ZA201306752B (da) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5893051B2 (ja) | 2011-02-10 | 2016-03-23 | クルセル ホランド ベー ヴェー | 交互空気圧膜式細胞分離システム |
| SG11201610143VA (en) * | 2014-06-04 | 2017-01-27 | Amgen Inc | Methods for harvesting mammalian cell cultures |
| JP6862001B2 (ja) * | 2015-08-20 | 2021-04-21 | サイティバ・スウェーデン・アクチボラグ | タンジェンシャルフローろ過システムにおいてろ過収量を向上させるための改良された方法 |
| CN106282001A (zh) * | 2016-10-09 | 2017-01-04 | 陈静 | 一种高通量快速捕获提纯循环肿瘤细胞的设备及方法 |
| CN107058086B (zh) * | 2017-05-26 | 2020-07-03 | 奥凯（苏州）生物技术有限公司 | 一种防止细胞堵塞培养器的方法及系统 |
| US10774297B2 (en) | 2017-08-03 | 2020-09-15 | Repligen Corporation | Method of actuation of an alternating tangential flow diaphragm pump |
| CN111565823B (zh) * | 2018-01-12 | 2023-03-31 | 富默乐有限公司 | 切向流过滤tff系统和包括集成泵装置的一次性tff单元 |
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- 2012-02-07 MX MX2013009030A patent/MX344465B/es active IP Right Grant
- 2012-02-07 BR BR112013019325-5A patent/BR112013019325B1/pt active IP Right Grant
- 2012-02-07 EA EA201391149A patent/EA023614B1/ru not_active IP Right Cessation
- 2012-02-07 EP EP12703101.1A patent/EP2673072B1/en active Active
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- 2012-02-07 CN CN201280007813.2A patent/CN103347595B/zh active Active
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| BR112013019325A2 (pt) | 2018-07-17 |
| NZ614364A (en) | 2015-07-31 |
| KR20140004150A (ko) | 2014-01-10 |
| ZA201306752B (en) | 2014-05-28 |
| JP5893051B2 (ja) | 2016-03-23 |
| IL227833A (en) | 2017-02-28 |
| EP2673072B1 (en) | 2014-11-19 |
| PL2673072T3 (pl) | 2015-04-30 |
| ES2529429T3 (es) | 2015-02-20 |
| EA201391149A1 (ru) | 2013-12-30 |
| US20140342446A1 (en) | 2014-11-20 |
| AU2012215514A1 (en) | 2013-08-29 |
| CN103347595B (zh) | 2016-01-20 |
| BR112013019325B1 (pt) | 2020-12-15 |
| WO2012107436A1 (en) | 2012-08-16 |
| MX2013009030A (es) | 2013-09-06 |
| EA023614B1 (ru) | 2016-06-30 |
| CA2824907C (en) | 2020-07-07 |
| US20130319944A1 (en) | 2013-12-05 |
| SG192611A1 (en) | 2013-09-30 |
| AU2012215514B2 (en) | 2017-04-20 |
| EP2673072A1 (en) | 2013-12-18 |
| MX344465B (es) | 2016-12-14 |
| CN103347595A (zh) | 2013-10-09 |
| CA2824907A1 (en) | 2012-08-16 |
| US8845902B2 (en) | 2014-09-30 |
| JP2014514129A (ja) | 2014-06-19 |
| KR101890000B1 (ko) | 2018-08-20 |
| IL227833A0 (en) | 2013-09-30 |
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