DK2588494T3 - Polypeptid med beta-glucosidaseaktivitet og anvendelser deraf - Google Patents

Polypeptid med beta-glucosidaseaktivitet og anvendelser deraf Download PDF

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Publication number
DK2588494T3
DK2588494T3 DK11729401.7T DK11729401T DK2588494T3 DK 2588494 T3 DK2588494 T3 DK 2588494T3 DK 11729401 T DK11729401 T DK 11729401T DK 2588494 T3 DK2588494 T3 DK 2588494T3
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Denmark
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polypeptide
sequence
cell
polynucleotide
seq
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DK11729401.7T
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English (en)
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Jong René Marcel De
Margot Elisabeth Francoise Schooneveld-Bergmans
Wilbert Herman Marie Heijne
Robbertus Antonius Damveld
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Dsm Ip Assets Bv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Claims (15)

1. Polypeptid, der omfatter aminosyresekvensen ifølge SEQ ID NO: 2 eller en aminosyresekvens, der kodes af nukleotidsekvensen ifølge SEQ ID NO: 1, eller et variantpolypeptid eller variantpolynukleotid deraf, hvor variantpolypeptidet har mindst 98 % sekvensidentitet med sekvensen ifølge SEQ ID NO: 2, eller variantpolynukleotidet koder for et polypeptid, der har mindst 98 % sekvensidentitet med sekvensen ifølge SEQ ID NO: 2, hvor polypeptidet har beta-glucosidaseaktivitet.
2. Polynukleotid, der omfatter: (a) nukleotidsekvensen ifølge SEQ ID NO: 1; eller (b) en nukleotidsekvens, der hybridiserer selektivt med et polynukleotid, der er det reverse komplement til SEQ ID NO: 1, under hybridiseringsbetingelser med 6X natriumchlorid/natriumcitrat (SSC) ved ca. 45 °C, efterfulgt af én eller flere vaske i 1 x SSC, 0,1 % SDS ved ca. 65 °C; eller (c) en nukleotidsekvens med mindst 90 % sekvensidentitet med nukleotidsekvensen ifølge SEQ ID NO: 1; eller (d) en nukleotidsekvens, der er det reverse komplement til en nukleotidsekvens ifølge (a) , (b) eller (c) , nukleotidet koder for et polypeptid ifølge krav 1.
3. Nukleinsyrekonstruktion, der omfatter polynukleotidet ifølge krav 2.
4. Vektor, der inkorporerer en polynukleotidsekvens ifølge krav 2.
5. Celle, der omfatter et polypeptid ifølge krav 1, et polynukleotid ifølge krav 2, en nukleinsyrekonstruktion ifølge krav 3 eller en vektor ifølge krav 4, hvilken celle er i stand til overekspression af polypeptidet.
6. Celle ifølge krav 5, hvor cellen er en eukaryot celle.
7. Celle ifølge krav 6, hvor cellen er en svampecelle.
8. Celle ifølge krav 7, hvor svampecellen er udvalgt fra gruppen, der består af slægterne Aspergillus, Trichoderma/Hypocrea, Fusarium, Disporotrichum, Penicillium, Acremonium, Neurospora, Thermoascus, Myceliophtora, Sporotrichum, Thielavia, Chryosporium, Fusarium, Humicola, Neurospora og Talaromyces.
9. Sammensætning, der omfatter: (i) et polypeptid ifølge krav 1 og; (ii) en cellulase en hemicellulase en pectinase og et polypeptid, der tilhører familien GH61.
10. Fremgangsmåde til fremstilling af et polypeptid ifølge krav 1 med carbohydratmaterialedegraderende og/eller carbohydrathydrolyserende aktivitet, hvilken fremgangsmåde omfatter dyrkning af en celle ifølge et hvilket som helst af kravene 5 til 8 under betingelser, der åbner mulighed for ekspression af polypeptidet, og eventuelt indvinding af det udtrykte polypeptid.
11. Fremgangsmåde til behandling af et substrat, der omfatter carbohydratmateriale, eventuelt et plantemateriale, hvilken fremgangsmåde omfatter etablering af kontakt mellem substratet og et polypeptid ifølge krav 1 og/eller en sammensætning ifølge krav 9.
12. Fremgangsmåde ifølge krav 11, hvor substratet er et plantemateriale, og plantematerialet er tilvejebragt i form af en plante, en plantepulp, et planteekstrakt, en fødevare eller bestanddel, der stammer derfra, eller et stof, tekstil eller beklædningsstykke, der omfatter et plantemateriale.
13. Fremgangsmåde ifølge krav 11 og/eller krav 12, hvor behandlingen omfatter degradering og/eller modificering af cellulose og/eller hemicellulose og/eller et pektinstof.
14. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter fremstilling af et fermenterbart sukker ved etablering af kontakt mellem lignocellulosemateriale og et polypeptid ifølge krav 1 og/eller en sammensætning ifølge krav 9 og fermentering af det resulterende fermenterbare sukker til fremstilling af et fermenteringsprodukt.
15. Anvendelse af et polypeptid ifølge krav 1 og/eller en sammensætning ifølge krav 9 til fremstilling af sukker fra et lignocellulosemateriale.
DK11729401.7T 2010-06-29 2011-06-23 Polypeptid med beta-glucosidaseaktivitet og anvendelser deraf DK2588494T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35959610P 2010-06-29 2010-06-29
EP10167776 2010-06-29
PCT/EP2011/060573 WO2012000890A1 (en) 2010-06-29 2011-06-23 Polypeptide having beta-glucosidase activity and uses thereof

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DK2588494T3 true DK2588494T3 (da) 2018-06-14

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DK17200641.3T DK3318574T3 (da) 2010-06-29 2011-06-23 Polypeptid med beta-glucosidase-aktivitet og anvendelser deraf
DK11729401.7T DK2588494T3 (da) 2010-06-29 2011-06-23 Polypeptid med beta-glucosidaseaktivitet og anvendelser deraf

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US (1) US9441214B2 (da)
EP (2) EP2588494B1 (da)
CN (1) CN102958939B (da)
AU (1) AU2011273688B2 (da)
BR (1) BR112012033699A2 (da)
CA (1) CA2803982A1 (da)
DK (2) DK3318574T3 (da)
EA (1) EA201300054A1 (da)
ES (1) ES2672125T3 (da)
HR (1) HRP20180854T1 (da)
HU (1) HUE037256T2 (da)
MX (1) MX2012015142A (da)
PL (1) PL2588494T3 (da)
WO (1) WO2012000890A1 (da)

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US9441214B2 (en) 2016-09-13
HUE037256T2 (hu) 2018-08-28
AU2011273688B2 (en) 2014-10-30
DK3318574T3 (da) 2021-07-12
HRP20180854T1 (hr) 2018-06-29
CA2803982A1 (en) 2012-01-05
EP3318574B1 (en) 2021-04-21
ES2672125T3 (es) 2018-06-12
EP2588494A1 (en) 2013-05-08
CN102958939B (zh) 2016-02-03
AU2011273688A1 (en) 2013-01-17
EA201300054A1 (ru) 2013-05-30
PL2588494T3 (pl) 2018-08-31
EP2588494B1 (en) 2018-03-07
CN102958939A (zh) 2013-03-06
EP3318574A1 (en) 2018-05-09
WO2012000890A1 (en) 2012-01-05
US20130104264A1 (en) 2013-04-25
MX2012015142A (es) 2013-05-01
BR112012033699A2 (pt) 2016-10-11

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