DK2577287T3 - Fremgangsmåde og instrumenter til omfattende multidimensionelle kromatografiske separationer ved anvendelse af en mikrostrømningsmodulator - Google Patents

Fremgangsmåde og instrumenter til omfattende multidimensionelle kromatografiske separationer ved anvendelse af en mikrostrømningsmodulator Download PDF

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DK2577287T3
DK2577287T3 DK11722783.5T DK11722783T DK2577287T3 DK 2577287 T3 DK2577287 T3 DK 2577287T3 DK 11722783 T DK11722783 T DK 11722783T DK 2577287 T3 DK2577287 T3 DK 2577287T3
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modulator
capillary
column
accumulation
channels
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DK11722783.5T
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Luigi Mondello
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Luigi Mondello
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
    • B01D53/02—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols by adsorption, e.g. preparative gas chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/463—Flow patterns using more than one column with serial coupling of separation columns for multidimensional chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns
    • G01N30/6039—Construction of the column joining multiple columns in series
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/16—Injection
    • G01N30/20—Injection using a sampling valve
    • G01N2030/201—Injection using a sampling valve multiport valves, i.e. having more than two ports
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/30—Control of physical parameters of the fluid carrier of temperature
    • G01N2030/3084—Control of physical parameters of the fluid carrier of temperature ovens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
    • G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7206—Mass spectrometers interfaced to gas chromatograph

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Claims (9)

1. Anordning til kromatografi, der omfatter: en modulator (10) med (i) en indgangsåbning (11, 37) til modtagelse af udstrømning fra en første kromatografisøjle (4), og (ii) to uafhængige åbninger (12, 16, henholdsvis. 36, 39) til modtagelse af en strøm fra den ene eller flere elektroniske tryk- og/eller strømningsstyringsenheder (7), der er placeret eksternt i forhold til modulatoren (10); enten én eller to akkumuleringskapillærprøvesløjfer (14), der er placeret eksternt i forhold til modulatoren (10), eller enten én eller to akkumuleringskapillærprøvesløjfer (14), der er integreret i modulatoren (10); hvor modulatoren (10) endvidere omfatter et system af integrerede kanaler, hvorved akkumuleringskapillærprøvesløjfen(-eme) er forbundet mellem den første (11, 36) og den anden (16, 39) uafhængige åbninger og med indgangsåbningen (11, 37); en injektor (9), der er direkte forbundet med modulatoren (10); kendetegnet ved, at anordningen endvidere omfatter: en nåleventil (29), der er integreret i modulatoren (10), til regulering af strømning, der forlader akkumuleringsprøvesløjfen/-sløjferne (14), og første og anden kanaler (16-17, 16-18, henholdsvis 38-40, 38-41), der er forbundet med akkumuleringskapillærprøvesløjfen(-erne) (14), hvor kanalerne er integreret i modulatoren (10), den første kanal endvidere forbundet med en anden kromatografisøjle (22), den anden kanal endvidere forbundet med en kapillærsøjle (19) for på en styret måle ved anvendelse af nåleventilen at opdele strømmen, der forlader akkumuleringskapillærsløjfen(-eme) (14), og for at frigøre overskydende tryk i den første kanal og som følge heraf i den anden kromatografisøjle (22) ved tømning af akkumuleringskapillærsløjfen(-eme) (14).
2. Anordning ifølge krav 1, der omfatter én eller to kapillærprøvesløjfer (14) placeret eksternt i forhold til modulatoren (10), kendetegnet ved et forhold mellem kapillærlængde og indre diameter på mellem 5 og 40.
3. Anordning ifølge krav 1, der omfatter én eller to indvendigt integrerede akkumuleringskanaler med et fast volumen.
4. Anordning ifølge krav 1, hvor der i systemet af integrerede kanaler er anbragt interne forbindelseskanaler i grupper og på en afbrudt måde og disse grupper er forbundet med hinanden via én eller to akkumuleringskapillærer eksternt i forhold til modulatoren (10).
5. Anordning ifølge krav 1, hvor der i systemet af integrerede kanaler er anbragt interne forbindelseskanaler på en kontinuerlig måde via en intern akkumuleringskanal.
6. Anordning ifølge krav 1, hvor de to kromatografisøjler (4, 22) er installeret i én eller flere gaskromatografiovne (1,6).
7. Anordning ifølge krav 1, hvor injektoren (9) er forbundet med en automatisk strømningsstyringsenhed eller med en automatisk trykstyringsenhed (7).
8. Anvendelse af en anordning ifølge krav 1 i en GCxGC-fremgangsmåde, der omfatter et massespektrometer ved udgangen af den anden søjle eller en hvilken som helst anden GC-detektor.
9. Anvendelse af en anordning ifølge krav 1 i endimensionel gaskromatografi som hurtigt højtryksinjektionssystem til prøver fordampet inde i injektoren (9), der er direkte forbundet med modulatoren (10), og når indgangsåbningen (11) er lukket.
DK11722783.5T 2010-05-27 2011-05-24 Fremgangsmåde og instrumenter til omfattende multidimensionelle kromatografiske separationer ved anvendelse af en mikrostrømningsmodulator DK2577287T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITME2010A000011A IT1400072B1 (it) 2010-05-27 2010-05-27 Metodo e apparecchiatura per cromatografia a separazione multidimensionale completa con micro modulatore a flusso bilanciato.
PCT/EP2011/058404 WO2011147801A1 (en) 2010-05-27 2011-05-24 Method and instrumentation for comprehensive multidimensional chromatography separations using a micro flow modulator

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US (1) US9067167B2 (da)
EP (2) EP3376221A1 (da)
DK (1) DK2577287T3 (da)
ES (1) ES2683031T3 (da)
HU (1) HUE039548T2 (da)
IT (1) IT1400072B1 (da)
PL (1) PL2577287T3 (da)
PT (1) PT2577287T (da)
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JP6672781B2 (ja) * 2015-12-22 2020-03-25 株式会社島津製作所 流路機構及びこれを備えた液体クロマトグラフ
WO2018112109A2 (en) 2016-12-15 2018-06-21 Leco Corporation Chromatography system
CN109580852B (zh) * 2019-02-03 2022-04-22 南京九岚纹仪器科技有限公司 一种全二维气相色谱仪及调制方法
GB2590418B (en) * 2019-12-17 2024-04-10 Agilent Technologies Inc Fluid separation with sampling unit selectively coupling upstream and downstream of separation unit
CN111239062B (zh) * 2020-02-04 2021-01-01 中国计量科学研究院 气体定量检测设备及方法

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PT2577287T (pt) 2018-09-28
US20130068100A1 (en) 2013-03-21
EP2577287A1 (en) 2013-04-10
HUE039548T2 (hu) 2019-01-28
ITME20100011A1 (it) 2011-11-28
ES2683031T3 (es) 2018-09-24
IT1400072B1 (it) 2013-05-17
US9067167B2 (en) 2015-06-30
TR201811105T4 (tr) 2018-08-27
EP3376221A1 (en) 2018-09-19
EP2577287B1 (en) 2018-05-09
PL2577287T3 (pl) 2018-12-31
WO2011147801A1 (en) 2011-12-01

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