DK2545150T3 - Fremgangsmåde - Google Patents

Fremgangsmåde Download PDF

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Publication number
DK2545150T3
DK2545150T3 DK11709190.0T DK11709190T DK2545150T3 DK 2545150 T3 DK2545150 T3 DK 2545150T3 DK 11709190 T DK11709190 T DK 11709190T DK 2545150 T3 DK2545150 T3 DK 2545150T3
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DK
Denmark
Prior art keywords
oil
leu
ser
gly
val
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DK11709190.0T
Other languages
English (en)
Inventor
Rene Mikkelsen
Tina Jørgensen
Jørn Borch Søe
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Dupont Nutrition Biosci Aps
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Publication of DK2545150T3 publication Critical patent/DK2545150T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
    • A23D9/04—Working-up
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/04—Refining fats or fatty oils by chemical reaction with acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/02—Refining fats or fatty oils by chemical reaction
    • C11B3/06—Refining fats or fatty oils by chemical reaction with bases
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/12—Refining fats or fatty oils by distillation
    • C11B3/14—Refining fats or fatty oils by distillation with the use of indifferent gases or vapours, e.g. steam
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01014—Chlorophyllase (3.1.1.14)

Claims (17)

  1. FREMGANGSMÅDE E Fremgangsmåde til raffinering af en planteolie, hvilken fremgangsmåde omfatter et trin med at bringe olien i kontakt med et enzym, der kan hydrolysere klorofyl eller et klorofylderivat, hvor enzymet bringes i kontakt med olien i tilstedeværelse af mindst 1 vægt-% phospholipid, og hvor enzymet bringes i kontakt med olien i tilstedeværelse af mindre end 0,2 vægt-% lysophosholipid, hvor enzymet bringes i kontakt med olien før eller under et trin med degummiering af olien.
  2. 2. Fremgangsmåde ifølge krav 1, hvor fremgangsmåden omfatter (a) at bringe enzymet i kontakt med olien før (b) degummiering af olien ved anvendelse af en phospholipase eller en acyltransferase.
  3. 3. Fremgangsmåde ifølge krav 1, hvor fremgangsmåden omfatter at bringe olien i kontakt med enzymet og en phospholipase C i et enkelt trin.
  4. 4. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor enzymet bringes i kontakt med olien ved en temperatur på mindre end 70 °C.
  5. 5. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor enzymet bringes i kontakt med olien i tilstedeværelse af 1 til 5 vægt-% vand.
  6. 6. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor degummieringstrinnet omfatter vanddegummiering.
  7. 7. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor degummieringstrinnet omfatter tilsætning af en syre til olien efterfulgt af neutralisering med et alkali.
  8. 8. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor fremgangsmåden ikke omfatter et trin med lerbehandling.
  9. 9. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor fremgangsmåden endvidere omfatter udførelse af et desodoriseringstrin for at frembringe en desodoriseret olie og et destillat.
  10. 10. Fremgangsmåde ifølge krav 8 eller krav 9, hvor fremgangsmåden frembringer et niveau af carotenoider og/eller tocopherol i den raffinerede olie og/eller destillatet, der er forhøjet sammenlignet med en fremgangsmåde, der omfatter et lerbehandlingstrin.
  11. 11. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor enzymet omfatter en chlorophyllase, pheophytinase, pyropheophytinase eller pheophytinpheophorbidhydrolase.
  12. 12. Fremgangsmåde ifølge et hvilket som helst foregående krav, hvor enzymet omfatter en polypeptidsekvens som defineret i en hvilken som helst af SEQ ID NO: 1, 2, 4, 6 eller 8 til 15, eller et funktionelt fragment eller variant deraf.
  13. 13. Fremgangsmåde ifølge krav 12, hvor enzymet omfatter en polypeptidsekvens med mindst 75 % sekvensidentitet med en hvilken som helst af SEQ ID NO: 1, 2, 4, 6 eller 8 til 15 over mindst 50 aminosyrerester.
  14. 14. Anvendelse af en fremgangsmåde som defineret i et hvilket som helst af kravene 8 til 13, til at øge et niveau af carotenoider og/eller tocopherol i en raffineret olie og/eller et destillat opnået ved desodorisering af olien.
  15. 15. Fremgangsmåde ifølge krav 4, hvor enzymet bringes i kontakt med olien ved en temperatur på 58 til 62 °C.
  16. 16. Fremgangsmåde ifølge krav 5, hvor enzymet bringes i kontakt med olien i tilstedeværelse af ca. 1 vægt-% vand.
  17. 17. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 13 eller 15, hvor enzymet bringes i kontakt med olien ved et pH på 6,3 til 6,5.
DK11709190.0T 2010-03-12 2011-02-23 Fremgangsmåde DK2545150T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31319410P 2010-03-12 2010-03-12
EP10156412 2010-03-12
PCT/IB2011/050742 WO2011110967A1 (en) 2010-03-12 2011-02-23 Process

Publications (1)

Publication Number Publication Date
DK2545150T3 true DK2545150T3 (da) 2018-11-19

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Application Number Title Priority Date Filing Date
DK11709190.0T DK2545150T3 (da) 2010-03-12 2011-02-23 Fremgangsmåde

Country Status (11)

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US (1) US20130059036A1 (da)
EP (1) EP2545150B1 (da)
CN (1) CN102844418B (da)
AR (1) AR080293A1 (da)
BR (1) BR112012022998A2 (da)
CA (1) CA2788148C (da)
DK (1) DK2545150T3 (da)
EA (1) EA201290903A1 (da)
MX (1) MX2012010399A (da)
PL (1) PL2545150T3 (da)
WO (1) WO2011110967A1 (da)

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Also Published As

Publication number Publication date
EP2545150B1 (en) 2018-07-25
CN102844418A (zh) 2012-12-26
BR112012022998A2 (pt) 2016-08-02
CA2788148C (en) 2018-03-13
EP2545150A1 (en) 2013-01-16
WO2011110967A1 (en) 2011-09-15
US20130059036A1 (en) 2013-03-07
CN102844418B (zh) 2015-11-25
MX2012010399A (es) 2012-10-05
PL2545150T3 (pl) 2019-01-31
AR080293A1 (es) 2012-03-28
CA2788148A1 (en) 2011-09-15
EA201290903A1 (ru) 2013-06-28

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