DK2222839T3 - Fremgangsmåder og sammensætninger til modulation af differentiering af pluripotente celler - Google Patents

Fremgangsmåder og sammensætninger til modulation af differentiering af pluripotente celler Download PDF

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Publication number
DK2222839T3
DK2222839T3 DK08856546.0T DK08856546T DK2222839T3 DK 2222839 T3 DK2222839 T3 DK 2222839T3 DK 08856546 T DK08856546 T DK 08856546T DK 2222839 T3 DK2222839 T3 DK 2222839T3
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Denmark
Prior art keywords
cells
mascs
nicd
cell
drcs
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DK08856546.0T
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English (en)
Inventor
Irina Aizman
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Sanbio Inc
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Publication of DK2222839T3 publication Critical patent/DK2222839T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (7)

1. Fremgangsmåde til begrænsning af potentialet af en marv-adhærerende stromal celle til differentiering til en celle af den mesenchymale linie, hvilken fremgangsmåde omfatter: (a) transfektion af marv-adhærerende stromale celler med en nukleinsyre indeholdende sekvenser kodende for et intracellulært Notch-domæne (NICD); (b) anbringelse af cellerne under selektion for nukleinsyren i trin (a); (c) standsning af selektionen efter et forudbestemt tidsrum; og (d) ekspandering af cellerne i trin (c).
2. Fremgangsmåde ifølge krav 1, ved hvilken udviklingen af den marv-adhærerende stromale celle til en osteogen linie er reduceret.
3. Fremgangsmåde ifølge krav 1, ved hvilken udviklingen af den marv-adhærerende stromale celle til en adipogen linie er reduceret.
4. Fremgangsmåde ifølge krav 1, ved hvilken udviklingen af den marv-adhærerende stromale celle til både osteogene og adipogene linier er reduceret.
5. Fremgangsmåde ifølge krav 1, ved hvilken NICD’et er et humant NICD.
6. Fremgangsmåde ifølge krav 5, ved hvilken NICD’et omfatter aminosyrer 1703-2504 af det humane Notch-protein.
7. Fremgangsmåde ifølge krav 1, ved hvilken det forudbestemte tidsrum er syv dage.
DK08856546.0T 2007-12-03 2008-12-03 Fremgangsmåder og sammensætninger til modulation af differentiering af pluripotente celler DK2222839T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US521107P 2007-12-03 2007-12-03
US12594108P 2008-04-30 2008-04-30
US13157708P 2008-06-10 2008-06-10
PCT/US2008/013299 WO2009073180A2 (en) 2007-12-03 2008-12-03 Methods and compositions for modulating differentiation of pluripotential cells

Publications (1)

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DK2222839T3 true DK2222839T3 (da) 2015-12-21

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DK08856546.0T DK2222839T3 (da) 2007-12-03 2008-12-03 Fremgangsmåder og sammensætninger til modulation af differentiering af pluripotente celler

Country Status (7)

Country Link
US (1) US20110306137A1 (da)
EP (3) EP3696261A1 (da)
DK (1) DK2222839T3 (da)
ES (2) ES2787513T3 (da)
PL (1) PL2222839T3 (da)
PT (1) PT2222839E (da)
WO (1) WO2009073180A2 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2270146A1 (en) 2002-02-06 2011-01-05 Sanbio, Inc. Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene
EP3851520A1 (en) * 2008-04-30 2021-07-21 SanBio, Inc. Neural regenerating cells with alterations in dna methylation
EP2806881A1 (en) 2012-01-27 2014-12-03 SanBio, Inc. Methods and compositions for modulating angiogenesis and vasculogenesis
AU2013263417B2 (en) 2012-05-16 2016-05-26 Sanbio, Inc. Methods and compositions for treatment of traumatic brain injury and for modulation of migration of neurogenic cells
AU2013330433B2 (en) 2012-10-09 2016-06-16 Sanbio, Inc. Methods and compositions for treatment of retinal degeneration
WO2016210256A1 (en) * 2015-06-25 2016-12-29 The University Of Florida Research Foundation, Inc. Conductive nonwoven mat and method of using the conductive nonwoven mat

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6528245B2 (en) 1998-05-07 2003-03-04 University Of South Florida Bone marrow cells as a source of neurons for brain and spinal cord repair
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7015037B1 (en) * 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
DE60129943T2 (de) 2000-02-11 2008-05-08 Philadelphia Health And Education Corp. Differenzierung von knochenmarkzellen in neuronale zellen und deren verwendungen
US20030003090A1 (en) 2001-05-31 2003-01-02 Prockop Darwin J. Directed in vitro differentiation of marrow stromal cells into neural cell progenitors
JP3886346B2 (ja) 2001-06-22 2007-02-28 サンバイオ，インコーポレイティド 骨髄間質細胞由来Ｓｃｈｗａｎｎ細胞を含む神経再生用医薬組成物
US7129034B2 (en) 2001-10-25 2006-10-31 Cedars-Sinai Medical Center Differentiation of whole bone marrow
EP2270146A1 (en) * 2002-02-06 2011-01-05 Sanbio, Inc. Method of inducing differentiation of bone marrow stromal cells to neural cells or skeletal muscle cells by introduction of notch gene
US20030181380A1 (en) * 2002-03-08 2003-09-25 Pear Warren S. Modulating lymphoid commitment and survival
US20040235165A1 (en) 2003-05-19 2004-11-25 Darwin Prockop In vitro differentiation of adult stem cells
CN1973031B (zh) * 2004-04-12 2011-11-02 桑比欧公司 具有神经元祖细胞特性的细胞

Also Published As

Publication number Publication date
EP2985341A2 (en) 2016-02-17
WO2009073180A3 (en) 2009-10-15
ES2551356T3 (es) 2015-11-18
PT2222839E (pt) 2015-11-30
EP3696261A1 (en) 2020-08-19
EP2985341A3 (en) 2016-06-01
PL2222839T3 (pl) 2016-02-29
ES2787513T3 (es) 2020-10-16
US20110306137A1 (en) 2011-12-15
EP2222839A4 (en) 2012-02-01
EP2985341B1 (en) 2020-04-01
WO2009073180A2 (en) 2009-06-11
EP2222839B1 (en) 2015-09-16
EP2222839A2 (en) 2010-09-01

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