DK2172548T3 - Phosphatidsyre-phosphatase-homologer og anvendelser deraf - Google Patents
Phosphatidsyre-phosphatase-homologer og anvendelser deraf Download PDFInfo
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- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 description 1
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- ZTUXEFFFLOVXQE-UHFFFAOYSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCCC(O)=O.CCCCCCCCCCCCCC(O)=O ZTUXEFFFLOVXQE-UHFFFAOYSA-N 0.000 description 1
- MLGCXEBRWGEOQX-UHFFFAOYSA-N tetradifon Chemical compound C1=CC(Cl)=CC=C1S(=O)(=O)C1=CC(Cl)=C(Cl)C=C1Cl MLGCXEBRWGEOQX-UHFFFAOYSA-N 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- RZWIIPASKMUIAC-VQTJNVASSA-N thromboxane Chemical compound CCCCCCCC[C@H]1OCCC[C@@H]1CCCCCCC RZWIIPASKMUIAC-VQTJNVASSA-N 0.000 description 1
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- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Claims (6)
1. Protein gengivet i et hvilket som helst af punkterne (a) til (e) nedenfor: (a) et protein bestående af aminosyresekvensen vist i SEQ ID NO: 2; (b) et protein, som består af en aminosyresekvens med deletering, substitution eller tilføjelse af 1 til 50 aminosyrer i SEQ ID NO:2, og som har phosphatidsyre-phosphata se-aktivitet; (c) et protein, som består af en aminosyresekvens med en similaritet på 85 % eller mere med aminosyresekvensen bestående af SEQ ID NO:2, og som har phospha-tidsyre-phosphatase-aktivitet; (d) et protein, som består af en aminosyresekvens med deletering, substitution eller tilføjelse af 1 til 50 aminosyrer i SEQ ID NO:2, og som har evnen til at yde en fedtsyrerate, der sikrer en højere andel af mindst én blandt (i) til (vi) vist nedenfor i fedtsyreraten hos en vært, som udtrykkeret protein bestående af aminosyresekvensen, end i fedtsyreraten hos en vært, som ikke udtrykker proteinet: (i) oliesyreindholdet; (ii) forholdet mellem palmitoleinsyreindholdet og palmitinsyreindholdet; (iii) forholdet mellem oliesyreindholdet og palmitinsyreindholdet; (iv) forholdet mellem det samlede indhold af stearinsyre og oliesyre på den ene side og palmitinsyre på den anden side; (v) forholdet mellem Cis-fedtsyreindholdet og Ci6-fedtsyreindholdet; og (vi) forholdet mellem indholdet af arachidonsyre, dihomo-y-linolensyre og/eller y-linolensyre; eller (e) et protein, som består af en aminosyresekvens med en similaritet på 85 % eller mere med aminosyresekvensen bestående af SEQ ID NO:2, og som har evnen til at yde en fedtsyrerate, der sikrer en højere andel af mindst én blandt (i) til (vi) vist nedenfor i fedtsyreraten hos en vært, som udtrykker et protein bestående af aminosyresekvensen, end i fedtsyreraten hos en vært, som ikke udtrykker proteinet: (i) oliesyreindholdet; (ii) forholdet mellem palmitoleinsyreindholdet og palmitinsyreindholdet; (iii) forholdet mellem oliesyreindholdet og palmitinsyreindholdet; (iv) forholdet mellem det samlede indhold af stearinsyre og oliesyre på den ene side og palmitinsyre på den anden side; (v) forholdet mellem Cis-fedtsyreindholdet og Ci6-fedtsyreindholdet; og (vi) forholdet mellem indholdet af arachidonsyre, dihomo-y-linolensyre og/eller y-linolensyre.
2. Nukleinsyre omfattende en nukleotidsekvens gengivet i et hvilket som helst af punkterne (a) til (i) nedenfor: (a) nukleotidsekvensen vist i SEQ ID NO:4; (b) nukleotidsekvensen vist i SEQ ID NO:l; (c) en nukleotidsekvens, som koder for et protein ifølge krav 1(a); (d) en nukleotidsekvens, som koder for et protein ifølge krav 1(b); (e) en nukleotidsekvens, som koder for et protein ifølge krav 1(c); (f) en nukleotidsekvens, som koder for et protein ifølge krav 1(d); (g) en nukleotidsekvens, som koder for et protein ifølge krav 1(e); (h) en nukleotidsekvens, som består af en nukleotidsekvens med 85 % eller mere similaritet med nukleotidsekvensen bestående af SEQ ID NO:4, og som koder for et protein med phosphatidsyre-phosphatase-aktivitet; eller (i) en nukleotidsekvens, som består af en nukleotidsekvens med 85 % eller mere similaritet med nukleotidsekvensen bestående af SEQ ID NO:4, og som koder for følgende protein: et protein, som har evnen til at yde en fedtsyrerate, der sikrer en højere andel af mindst én blandt (i) til (vi) vist nedenfor i fedtsyreraten hos en vært, som udtrykker proteinet, end i fedtsyreraten hos en vært, som ikke udtrykker proteinet: (i) oliesyreindholdet; (ii) forholdet mellem palmitoleinsyreindholdet og palmitinsyreindholdet; (iii) forholdet mellem oliesyreindholdet og palmitinsyreindholdet; (iv) forholdet mellem det samlede indhold af stearinsyre og oliesyre på den ene side og palmitinsyre på den anden side; (v) forholdet mellem Cis-fedtsyreindholdet og Ci6-fedtsyreindholdet; og (vi) forholdet mellem indholdet af arachidonsyre, dihomo-y-linolensyre og/eller y-linolensyre.
3. Nukleinsyre ifølge krav 2 omfattende en nukleotidsekvens vist i et hvilket som helst af punkterne (a) til (d) nedenfor: (a) nukleotidsekvens ifølge krav 2(d), hvor antallet af aminosyrer, der skal deleteres, substitueres eller tilføjes i proteinet ifølge krav 1(b) er 1 til 10; (b) nukleotidsekvens ifølge krav 2(e), hvor aminosyresekvensen af proteinet ifølge krav 1(c) har en similaritet på 90 % eller mere med aminosyresekvensen bestående af SEQ ID NO:2: (c) nukleotidsekvens ifølge krav 2(f), hvor antallet af aminosyrer, der skal deleteres, substitueres eller tilføjes i proteinet ifølge krav 1(d) er 1 til 10; eller (d) nukleotidsekvens ifølge krav 2(g), hvor aminosyresekvensen af proteinet ifølge krav 1(e) har en similaritet på 90 % eller mere med aminosyresekvensen bestående af SEQ ID NO:2.
4. Rekombinant vektor omfattende nukleinsyren ifølge krav 2 eller 3.
5. Transformant, som er transformeret med den rekombinante vektor ifølge krav 4.
6. Fremgangsmåde til fremstilling af en fedtsyresammensætning, hvilken fremgangsmåde omfatter at dyrke transformanten ifølge krav 5 og at indvinde fedtsyresammensætningen indeholdt i det dyrkede produkt opnået ved dyrkning af transformanten ifølge krav 5.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2007181899 | 2007-07-11 | ||
| PCT/JP2008/062450 WO2009008466A1 (ja) | 2007-07-11 | 2008-07-10 | ホスファチジン酸ホスファターゼホモログとその利用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2172548T3 true DK2172548T3 (da) | 2017-01-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK08778022.7T DK2172548T3 (da) | 2007-07-11 | 2008-07-10 | Phosphatidsyre-phosphatase-homologer og anvendelser deraf |
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| Country | Link |
|---|---|
| US (1) | US20100196579A1 (da) |
| EP (1) | EP2172548B1 (da) |
| JP (1) | JP5180960B2 (da) |
| KR (1) | KR101519678B1 (da) |
| CN (1) | CN101595216B (da) |
| AU (1) | AU2008273264B2 (da) |
| BR (1) | BRPI0814699A2 (da) |
| CA (1) | CA2692813C (da) |
| DK (1) | DK2172548T3 (da) |
| RU (1) | RU2507264C2 (da) |
| WO (1) | WO2009008466A1 (da) |
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| US9447393B2 (en) | 2011-07-29 | 2016-09-20 | Suntory Holdings Limited | Phosphatidic acid phosphatase gene |
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| JP4149007B2 (ja) | 1995-02-01 | 2008-09-10 | 麒麟麦酒株式会社 | 酵母の凝集性判定用ｄｎａ分子および凝集性判定方法 |
| US6476294B1 (en) * | 1998-07-24 | 2002-11-05 | Calgene Llc | Plant phosphatidic acid phosphatases |
| JP4231170B2 (ja) | 1999-10-26 | 2009-02-25 | サントリー株式会社 | 酵母の育種方法 |
| EP1236803A4 (en) | 1999-11-30 | 2003-05-02 | Asahi Breweries Ltd | METHOD FOR EVALUATING THE FLAKE OUT PROPERTIES OF LOW BREWER'S YEAST |
| WO2003020936A1 (en) * | 2001-08-31 | 2003-03-13 | The Dow Chemical Company | Nucleic acid compositions conferring altered metabolic characteristics |
| US7314974B2 (en) * | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
| US20070072275A1 (en) | 2003-08-22 | 2007-03-29 | Misa Ochiai | Method for breeding lipid-producing fungus |
| JP4481702B2 (ja) * | 2004-03-31 | 2010-06-16 | サントリーホールディングス株式会社 | 脂質生産菌の育種方法およびその利用 |
| WO2007129770A2 (en) * | 2006-05-08 | 2007-11-15 | Suntory Limited | Fatty acid synthetase, polynucleotide encoding the same, and uses thereof |
| EP2169055B1 (en) * | 2007-05-25 | 2015-04-01 | Suntory Holdings Limited | Novel lysophosphatidate acyltransferase gene |
| CA2685713C (en) * | 2007-06-18 | 2015-03-31 | Suntory Holdings Limited | Glycerol-3-phosphate acyltransferase (gpat) homologs and use thereof |
| US9447393B2 (en) * | 2011-07-29 | 2016-09-20 | Suntory Holdings Limited | Phosphatidic acid phosphatase gene |
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- 2008-07-10 RU RU2010104678/10A patent/RU2507264C2/ru not_active IP Right Cessation
- 2008-07-10 BR BRPI0814699-3A2A patent/BRPI0814699A2/pt not_active Application Discontinuation
- 2008-07-10 AU AU2008273264A patent/AU2008273264B2/en not_active Ceased
- 2008-07-10 CA CA2692813A patent/CA2692813C/en not_active Expired - Fee Related
- 2008-07-10 JP JP2009522666A patent/JP5180960B2/ja not_active Expired - Fee Related
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- 2008-07-10 CN CN2008800032611A patent/CN101595216B/zh not_active Expired - Fee Related
- 2008-07-10 US US12/520,634 patent/US20100196579A1/en not_active Abandoned
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| Publication number | Publication date |
|---|---|
| AU2008273264A1 (en) | 2009-01-15 |
| CA2692813A1 (en) | 2009-01-15 |
| CN101595216A (zh) | 2009-12-02 |
| CA2692813C (en) | 2017-01-24 |
| JPWO2009008466A1 (ja) | 2010-09-09 |
| WO2009008466A1 (ja) | 2009-01-15 |
| RU2507264C2 (ru) | 2014-02-20 |
| RU2010104678A (ru) | 2011-08-20 |
| EP2172548B1 (en) | 2016-12-21 |
| JP5180960B2 (ja) | 2013-04-10 |
| KR101519678B1 (ko) | 2015-05-12 |
| US20100196579A1 (en) | 2010-08-05 |
| CN101595216B (zh) | 2013-01-09 |
| EP2172548A4 (en) | 2010-09-08 |
| AU2008273264B2 (en) | 2013-07-04 |
| KR20100041777A (ko) | 2010-04-22 |
| BRPI0814699A2 (pt) | 2014-10-21 |
| EP2172548A1 (en) | 2010-04-07 |
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