DK2142642T3 - Fremgangsmåder og middel til stamcelleproliferation og efterfølgende generering og ekspansion af progenitorceller, såvel som produktion af effektorceller som kliniske terapeutika - Google Patents
Fremgangsmåder og middel til stamcelleproliferation og efterfølgende generering og ekspansion af progenitorceller, såvel som produktion af effektorceller som kliniske terapeutika Download PDFInfo
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- DK2142642T3 DK2142642T3 DK08723925.7T DK08723925T DK2142642T3 DK 2142642 T3 DK2142642 T3 DK 2142642T3 DK 08723925 T DK08723925 T DK 08723925T DK 2142642 T3 DK2142642 T3 DK 2142642T3
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Claims (19)
1. Fremgangsmåde til ekspansion og differentiering af hæmopoietiske progenitorceller, der omfatter dyrkning af celler fra en prøve, der omfatter stamceller, progenitorceller eller begge, fra humant postembryonalt væv i et ekspansionsmedium, der omfatter et celledyrkningsmedium, 1-100 mg/1 desulfateret heparin (UFH) og/eller lavmolekylær heparin (LMWH) med en middelmolekylvægt på mellem ca. 2000-10000 dalton, en indsamling af cytokiner, hvor indsamlingen af cytokiner omfatter TPO, FLT-3L, SCF og IL-7, og eventuelt endvidere omfatter IL-3, i konventionelle mængder og desuden GM-CSF, G-CSF, LIF, ΜΙΡ-Ια og IL-6, i fysiologiske mængder, og endvidere konventionelle tilskud, og dyrkning af cellerne yderligere i et ekspansions- og differentieringsmedium, der omfatter en indsamling af cytokiner, 1-100 mg/1 desulfateret heparin (UFH) og/eller lavmolekylær heparin (LMWH) med en middelmolekylvægt på mellem ca. 2000-10000 dalton og humant serum, hvor indsamlingen af cytokiner omfatter TPO, FLT-3L, SCF, IL-7, IL-2, IL-15, og desuden GM-CSF, G-CSF, LIF, ΜΙΡ-Ια og IL-6, hvor de dyrkede celler ekspanderes og differentieres.
2. Fremgangsmåde ifølge krav 1, hvor ekspansionsmediet endvidere omfatter lithium.
3. Fremgangsmåde ifølge krav 2, hvor skiftet til ekspansions- og differentieringsmedium opnås ved trinvis udskiftning og/eller fortynding af dyrkningsmedium med ekspansions- og differentieringsmedium.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1-3, hvor koncentrationen af FLT-3, TPO og/eller SCF reduceres under dyrkning.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvor lithium og/eller UFH reduceres under dyrkning.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1-5, der endvidere omfatter indsamling af kulturceller.
7. Indsamling ex vivo af celler, der kan opnås ved hjælp af en fremgangsmåde ifølge kravene 1-6 og som omfatter 109 naturlige dræberceller fra en enkelt donor.
8. Indsamling ex vivo af celler ifølge krav 7, der omfatter prolifererede, bevarede stamceller eller begge.
9. Indsamling ex vivo af celler ifølge krav 7, der omfatter naturlige dræberprogenitorceller.
10. Indsamling ex vivo af celler ifølge krav 7, hvor cellerne i alt væsentligt er genetisk identiske.
11. Indsamling ex vivo af celler ifølge krav 7, hvor cellerne er immunologisk funktionelle.
12. Anvendelse af en indsamling af celler ifølge et hvilket som helst af kravene 7-11 til fremstilling af et medikament.
13. Anvendelse af en indsamling af celler ifølge et hvilket som helst af kravene 7-11 til fremstilling af et medikament til behandling af en person, der lider af en tumor, en virusinfektion eller begge.
14. Anvendelse af en indsamling af celler ifølge et hvilket som helst af kravene 7-11 til fremstilling af et medikament til behandling af en person, der lider af en autoimmun sygdom, transplantatafvisning eller mistet graviditet.
15. Kit af dele til at generere naturlige dræberceller ud af progenitorceller, stamceller eller begge, hvor kittet omfatter ct ekspansions- og diffcrcnticringsmcdium ifølge krav 1, og/cllcr bestanddelene deraf i mængder, der er tilstrækkelige til at fremstille mediet.
16. Kit af dele ifølge krav 15, der endvidere omfatter et ekspansionsmedium ifølge krav 1, ogAllcr bestanddelene deraf i mængder, der er tilstrækkelige til at fremstille mediet.
17. Fremgangsmåde ifølge et hvilket som helst af kravene 1-6, hvor cellerne fra prøven er somatiske stamceller fra humant postembryonalt væv.
18. Fremgangsmåde ifølge krav 17, hvor efter 5 til 15 dages dyrkning af cellerne i ekspansionsmediet ekspansionsmediet udskiftes med ekspansions- og differentieringsmediet.
19. Fremgangsmåde ifølge krav 18, hvor cellerne differentieres til NK-celler.
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| EP07105060 | 2007-03-27 | ||
| PCT/NL2008/050174 WO2008118020A1 (en) | 2007-03-27 | 2008-03-27 | Methods and means for stem cell proliferation and subsequent generation and expansion of progenitor cells, as well as production of effector cells as clinical therapeutics |
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| CN101878034B (zh) | 2007-09-28 | 2013-11-20 | 细胞基因细胞疗法公司 | 使用人胎盘灌洗液和人来自胎盘的中间体自然杀伤细胞的肿瘤抑制 |
| WO2012009422A1 (en) | 2010-07-13 | 2012-01-19 | Anthrogenesis Corporation | Methods of generating natural killer cells |
| US11118165B2 (en) * | 2011-03-18 | 2021-09-14 | Glycostem Therapeutics B.V. | Generation of NK cells and NK-cell progenitors |
| WO2013119118A1 (en) * | 2012-02-08 | 2013-08-15 | Ipd-Therapeutics B.V. | Ex vivo nk cell differentiation from cd34+ hematopoietic cells |
| US9664671B2 (en) | 2012-07-24 | 2017-05-30 | Nissan Chemical Industries, Ltd. | Culture medium composition and method of culturing cell or tissue using thereof |
| CA2881792C (en) * | 2012-08-13 | 2024-02-27 | Anthrogenesis Corporation | Natural killer cells and uses thereof |
| US10017805B2 (en) | 2012-08-23 | 2018-07-10 | Nissan Chemical Industries, Ltd. | Enhancing ingredients for protein production from various cells |
| EP3622960A1 (en) | 2013-02-05 | 2020-03-18 | Celularity, Inc. | Natural killer cells from placenta |
| WO2014171486A1 (ja) * | 2013-04-17 | 2014-10-23 | 日産化学工業株式会社 | 培地組成物及び当該培地組成物を用いた赤血球の製造方法 |
| CN108239621B (zh) * | 2018-01-24 | 2020-08-18 | 北京臻溪谷医学研究中心（有限合伙） | 一种胎盘亚全能干细胞分离、扩增、冻存、复苏干细胞的方法 |
| JP7268039B2 (ja) | 2018-02-01 | 2023-05-02 | エヌケーマックス カンパニー リミテッド | がん治療のためのナチュラルキラー細胞および組成物の製造方法 |
| CN110247106B (zh) * | 2019-05-25 | 2021-11-19 | 浙江锋锂新能源科技有限公司 | 一种带有铌酸钛的混合固液电解质电池 |
| WO2021178890A1 (en) | 2020-03-06 | 2021-09-10 | Sorrento Therapeutics, Inc. | Innate immunity killer cells targeting psma positive tumor cells |
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| US5087570A (en) * | 1988-05-10 | 1992-02-11 | Weissman Irving L | Homogeneous mammalian hematopoietic stem cell composition |
| EP1066050B2 (de) * | 1998-03-27 | 2010-06-02 | Gabriele Prof. Dr. Multhoff | Verwendung von hsp70 protein |
| WO2005105982A1 (de) | 2004-03-31 | 2005-11-10 | Reinhardt Schwartz-Albiez | Verfahren zur sequentiellen ex vivo expansion humaner postembryonaler stammzellen mit nachfolgender selektiver differenzierung |
| EP1809738B1 (en) * | 2004-11-02 | 2014-03-05 | THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services | Compositions and methods for treating hyperproliferative disorders |
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| CA2682243C (en) | 2019-07-23 |
| PL2142642T3 (pl) | 2017-10-31 |
| EP2142642A1 (en) | 2010-01-13 |
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| WO2008118020A1 (en) | 2008-10-02 |
| CA2682243A1 (en) | 2008-10-02 |
| EP2142642B1 (en) | 2017-08-09 |
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| US9193953B2 (en) | 2015-11-24 |
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