DK2109681T3 - Nye mikroorganismer til fremstilling af 1,2-propandiol opnået ved en kombination af evolution og rationelt design - Google Patents

Nye mikroorganismer til fremstilling af 1,2-propandiol opnået ved en kombination af evolution og rationelt design Download PDF

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DK2109681T3
DK2109681T3 DK08714246.9T DK08714246T DK2109681T3 DK 2109681 T3 DK2109681 T3 DK 2109681T3 DK 08714246 T DK08714246 T DK 08714246T DK 2109681 T3 DK2109681 T3 DK 2109681T3
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strain
gene
propanediol
coli
evolved
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DK08714246.9T
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Philippe Soucaille
Francois Voelker
Rainer Figge
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Metabolic Explorer Sa
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B10/00—Directed molecular evolution of macromolecules, e.g. RNA, DNA or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (8)

1. Fremgangsmåde til fremstilling af en evolveret stamme af mikroorganismer til fremstilling af 1,2-propandiol fra en kulstofkilde, hvilken fremgangsmåde omfatter: - dyrkning af en initial stamme under selektionstryk i et passende vækstmedium, hvor den initiale bakteriestamme omfatter en attenuation af ekspressionen af fp/A-genet og en attenuation af ekspressionen af mindst ét i omdannelsen af methylglyoxal til lactat involveret gen for at fremme evolutionen i den initiale stamme, - derefter udvælgelse og isolering af den evolverede stamme med forhøjet 1,2-propandiol-produktionshastighed, - derefter rekonstruering af et funktionelt ip/A-gen i den evolverede stamme.
2. Fremgangsmåde ifølge krav 1, hvorved det i omdannelsen af methylglyoxal til lactat involverede gen er udvalgt fra gruppen bestående af: gloA, aldA og aldB og kombinationer deraf.
3. Fremgangsmåde ifølge kravene 1 til 2, hvorved den initiale stamme endvidere omfatter attenuation af ekspressionen af mindst ét af de fra gruppen bestående af IdhA, pfIA, pilB, adhE, eddog eda udvalgte gener.
4. Fremgangsmåde ifølge kravene 1 til 3, hvorved den initiale stamme endvidere omfatter attenuation af mindst ét gen udvalgt fra gruppen bestående af arcA og ndh.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, hvorved den evolverede stamme er udvalgt og isoleret på basis af dens 1,2-propandiol-produktionshastighed, forhøjet med mindst 20 % i sammenligning med produktionshastigheden for den initiale stamme.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 5, hvorved den initiale stamme er udvalgt fra gruppen bestående af bakterier, gær og svampe.
7. Fremgangsmåde ifølge krav 6, hvorved den initiale stamme er udvalgt fra gruppen bestående af Enterobacteriaceae, Bacillaceae, Clostridiaceae, Streptomycetaceae og Corynebacteriaceae.
8. Fremgangsmåde ifølge krav 7, hvorved den initiale stamme er enten Escherichia coli eller Clostridium acetobutylicum.
DK08714246.9T 2007-03-23 2008-03-21 Nye mikroorganismer til fremstilling af 1,2-propandiol opnået ved en kombination af evolution og rationelt design DK2109681T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IB2007001680 2007-03-23
PCT/EP2008/053445 WO2008116852A1 (en) 2007-03-23 2008-03-21 New micro-organisms for the production of 1,2-propanediol obtained by a combination of evolution and rational design.

Publications (1)

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DK2109681T3 true DK2109681T3 (da) 2014-12-08

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DK08714246.9T DK2109681T3 (da) 2007-03-23 2008-03-21 Nye mikroorganismer til fremstilling af 1,2-propandiol opnået ved en kombination af evolution og rationelt design

Country Status (14)

Country Link
US (1) US8298807B2 (da)
EP (1) EP2109681B1 (da)
JP (1) JP5570821B2 (da)
KR (1) KR101528943B1 (da)
CN (1) CN101641446A (da)
AR (1) AR066196A1 (da)
BR (1) BRPI0808972B1 (da)
CA (1) CA2679989C (da)
DK (1) DK2109681T3 (da)
ES (1) ES2525796T3 (da)
IL (1) IL200705A0 (da)
MX (1) MX2009010229A (da)
TW (1) TW200904984A (da)
WO (1) WO2008116852A1 (da)

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US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
MX2011004842A (es) 2008-11-07 2011-05-30 Metabolic Explorer Sa Uso de sacarosa como sustrato para la produccion fermentativa de 1,2-propanodiol.
EP2233562A1 (en) 2009-03-24 2010-09-29 Metabolic Explorer Method for producing high amount of glycolic acid by fermentation
WO2010141920A2 (en) * 2009-06-04 2010-12-09 Genomatica, Inc. Microorganisms for the production of 1,4-butanediol and related methods
EP2459720B1 (en) 2009-07-30 2016-04-27 Metabolic Explorer Mutant glycerol dehydrogenase (glydh) for the production of a biochemical by fermentation
IN2012DN00302A (da) 2009-07-30 2015-05-08 Metabolic Explorer Sa
US8969053B2 (en) 2009-07-30 2015-03-03 Metabolic Explorer Mutant YqhD enzyme for the production of a biochemical by fermentation
EP2532751A1 (en) * 2011-06-10 2012-12-12 Metabolic Explorer Use of inducible promoters in the fermentative production of 1,2-propanediol
US9353386B2 (en) 2011-06-15 2016-05-31 B.R.A.I.N. Biotechnology Research And Information Network Ag Means and methods for producing propanediol
EP3050970B1 (en) 2015-01-28 2019-09-18 Metabolic Explorer Modified microorganism for optimized production of 1,4-butanediol
CN107690482B (zh) 2015-04-07 2022-11-29 代谢探索者公司 用于2,4-二羟基丁酸的优化生产的经修饰的微生物
ES2811337T3 (es) 2015-04-07 2021-03-11 Metabolic Explorer Sa Microorganismo modificado para la producción optimizada de 2,4-dihidroxibutirato con eflujo de 2,4- dihidroxibutirato aumentado
EP3331998B1 (en) 2015-08-07 2020-06-24 Evonik Operations GmbH Protein thiocarboxylate-dependent l-methionine production by fermentation
EP3347478B1 (en) 2015-09-10 2022-09-21 Metabolic Explorer New lactaldehyde reductases for the production of 1,2-propanediol
DK3365427T3 (da) 2015-10-23 2020-10-26 Metabolic Explorer Sa Mikroorganisme, der er modificeret til assimilation af levulinsyre
US11034985B2 (en) 2015-11-27 2021-06-15 Evonik Operations Gmbh Method for producing L-methionine
KR20190026851A (ko) 2016-07-08 2019-03-13 에보니크 데구사 게엠베하 당 포스포트랜스퍼라제 시스템 (pts)을 코딩하는 유전자를 포함하는 미생물에 의한 메티오닌 또는 그의 히드록시 유사체 형태의 발효적 생산을 위한 방법
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
EP3342873A1 (en) 2016-12-29 2018-07-04 Metabolic Explorer Conversion of methylglyoxal into hydroxyacetone using enzymes and applications thereof
EP3470512A1 (en) 2017-10-10 2019-04-17 Metabolic Explorer Mutant phosphoserine aminotransferase for the conversion of homoserine into 4-hydroxy-2-ketobutyrate
KR102245274B1 (ko) 2019-01-28 2021-04-27 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 최소 유전체를 갖는 신규 미생물 및 이의 제조 방법

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US6087140A (en) * 1997-02-19 2000-07-11 Wisconsin Alumni Research Foundation Microbial production of 1,2-propanediol from sugar
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
EP1597364B1 (fr) 2003-02-18 2013-07-24 Metabolic Explorer Procede de preparation de microorganismes evolues permettant la creation ou la modification de voies metaboliques
FR2864967B1 (fr) * 2004-01-12 2006-05-19 Metabolic Explorer Sa Microorganisme evolue pour la production de 1,2-propanediol

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Publication number Publication date
KR101528943B1 (ko) 2015-06-15
EP2109681B1 (en) 2014-10-15
EP2109681A1 (en) 2009-10-21
MX2009010229A (es) 2009-10-26
BRPI0808972A2 (pt) 2014-08-26
CN101641446A (zh) 2010-02-03
JP5570821B2 (ja) 2014-08-13
AR066196A1 (es) 2009-08-05
CA2679989A1 (en) 2008-10-02
KR20100015810A (ko) 2010-02-12
WO2008116852A1 (en) 2008-10-02
BRPI0808972B1 (pt) 2016-11-29
ES2525796T3 (es) 2014-12-30
US20100285547A1 (en) 2010-11-11
IL200705A0 (en) 2010-05-17
US8298807B2 (en) 2012-10-30
CA2679989C (en) 2015-07-07
TW200904984A (en) 2009-02-01
JP2010521958A (ja) 2010-07-01

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