DK200200302A - Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........ - Google Patents

Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........ Download PDF

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DK200200302A
DK200200302A DK200200302A DKPA200200302A DK200200302A DK 200200302 A DK200200302 A DK 200200302A DK 200200302 A DK200200302 A DK 200200302A DK PA200200302 A DKPA200200302 A DK PA200200302A DK 200200302 A DK200200302 A DK 200200302A
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glu
cys
phe
gly
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DK200200302A
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George John Broze Jr
Kretzmer Kuniko Kusano
Wun Tze-Chein
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Monsanto Co
Univ Washington
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Priority claimed from US07/123,753 external-priority patent/US4966852A/en
Priority claimed from DK200001612A external-priority patent/DK174754B1/da
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Priority to DK200200302A priority Critical patent/DK175430B1/da
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PATENTKRAV 1. Rekombinant polypeptid omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI) som vist i Fig. 3. 2. Polypeptid ifølge krav 1 hvori den modne vævsfaktorinhibitor består af aminosyrerne 29-304 som vist i Fig. 3, 3. Polypeptid ifølge krav 1, som yderligere omfatter en forlænget ami-nosyresekvens umiddelbart forud for den modne vævsfaktorinhibitor. 4. Polypeptid bestående af moden vævsfaktorinhibitor (aminosyrerne 29-304) med en umiddelbart forudgående N-terminal methioninrest, alanin-rest eller methionin-alanin-dipeptid. 5. Renset og isoleret polypeptid omfattende en aminosyresekvens valgt blandt sekvenserne: (a) Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys; (b) Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys; (c) Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys; (d) Leu Lys Leu Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys Thr Arg Asp Asn Ala Asn Arg Ile Ile Lys Thr Thr Leu; (e) Gin Gin Glu Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly Pro Asn Gly Phe Gin Val Asp Asn Tyr Gly; og (f) Glu Phe His Gly Pro Ser Trp Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys Lys Lys Gly Phe Ile Gin Arg Ile Ser Lys Gly Gly Leu. 6, Renset og isoleret polypeptid med en aminosyresekvens valgt blandt sekvenserne: (a) Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys; (b) Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys; og (c) Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys. 7. Rekombinant polypeptid med aminosyresekvensen;
Asp Ser Glu Glu Asp Glu Glu His Thr Ile Ile Thr Asp Thr Glu Leu Pro Pro Leu Lys Leu Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Ley Glu Glu Cys Lys Lys Met Cys Thr Arg Asp
Asn Ala Asn Arg Ile Ile Lys Thr Thr Leu Gin Gin Glu Lys Pro Asp Phe Cys Phe Leu Glu Giu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly Pro Asn Gly Phe Gin Val Asp Asn Tyr Gly Thr Gin Leu Asn Ala Val Asn Asn Ser Leu Thr Pro Gin Ser Thr Lys Val Pro Ser Leu Phe Glu Phe His Gly Pro Ser Trp Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys Lys Lys Gly Phe Ile Gin Arg Ile Ser Lys Gly Gly Leu Ile Lys Thr Lys Arg Lys Arg Lys Lys Gin Arg Val Lys Ile Ala Tyr Glu Glu Ile Phe Val Lys Asn Met. 8. Renset og isoleret polypeptid med aminosyresekvensen:
Asp Ser Glu Glu Asp Glu Glu His Thr Ile Ile Thr Asp Thr Glu Leu Pro Pro Leu Lys Leu Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys Thr Arg Asp Asn Ala Asn Arg Ile Ile Lys Thr Thr Leu Gin Glu Glu Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly Pro Asn Gly Phe Gin Val Asp Asn Tyr Gly Thr Gin Leu Asn Ala Val Asn Asn Ser Leu Thr Pro Gin Ser Thr Lys Val Pro Ser Leu Phe Glu Phe His Gly Pro Ser Trp Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys
Leu Arg Ala Cys Lys Lys Gly Phe Ile Gin Arg Ile Ser Lys Gly Gly Leu Ile Lys Thr Lys Arg Lys Arg Lys Lys Gin Arg Val Lys Ile Ala Tyr Glu Glu Ile Phe Val Lys Asn Met. 9. Ekspressionsvektor omfattende en renset og isoleret DNA, som koder for moden vævsfaktorinhibitor (TFI), som vist i Fig. 3. 10. Ekspressionsvektor omfattende en renset og isoleret DNA, som koder for et polypeptid valgt blandt: (a) Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys; (b) Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys; (c) Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys; (d) Leu Lys Leu Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Gly Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe Thr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys Thr Arg Asp Asn Ala Asn Arg Ile Ile Lys Thr Thr Leu; (e) Gin Gin Glu Lys Pro Asp Phe Cys Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Gin Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly Pro Asn Gly Phe Gin Val Asp Asn Tyr Gly; og (f) Glu Phe His Gly Pro Ser Trp Cys Leu Thr Pro Ala Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile Gly Lys Cys Arg Pro Phe Lys Tyr Ser Gly Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys Lys Lys Gly Phe Ile Gin Arg Ile Ser Lys Gly Gly Leu. 11. Ekspressionsvektor omfattende en renset og isoleret DNA, som koder for et polypeptid med sekvensen:
Asp Ser Glu Glu Asp Glu Glu His Thr Ile Ile Thr Asp Thr Glu Leu Pro Pro Leu Lys Leu Met His Ser Phe Cys Ala Phe Lys Ala Asp Asp Lys Pro Cys Lys Ala Ile Met Lys Arg Phe Phe Phe Asn Ile Phe THr Arg Gin Cys Glu Glu Phe Ile Tyr Gly Ala Cys Glu Gly Asn Gin Asn Arg Phe Glu Ser Leu Glu Glu Cys Lys Lys Met Cys Thr Arg Asp Asn ALa Asn Arg Ile Ile Lys Thr Thr Leu Gin Gin Glu Lys Pro Asp Phe CYs Phe Leu Glu Glu Asp Pro Gly Ile Cys Arg Gly Ile Cys Arg Gly Tyr Ile Thr Arg Tyr Phe Tyr Asn Asn Glu Thr Lys Gin Cys Glu Arg Phe Lys Tyr Gly Gly Cys Leu Gly Asn Met Asn Asn Phe Glu Thr Leu Glu Glu Cys Lys Asn Ile Cys Glu Asp Gly Pro Asn Gly Phe Gin Val Asp Asn Tyr Gly Thr Gin Leu Asn Ala Val Asn Asn Ser Leu Thr Pro Gin Ser Thr Lys Val Pro Ser Leu Phe GLu Phe His Gly Pro Ser Trp Cys Leu Thr Pro ALa Asp Arg Gly Leu Cys Arg Ala Asn Glu Asn Arg Phe Tyr Tyr Asn Ser Val Ile GLy Lys Cys Arg Pro Phe Lys Tyr Ser GLy Cys Gly Gly Asn Glu Asn Asn Phe Thr Ser Lys Gin Glu Cys Leu Arg Ala Cys Lys Lys Gly Phe Ile Gin Arg Ile Ser Lys Gly Gly Leu Ile Lys Thr Lys Arg Lys Arg Lys Lys Gin ArG Val Lys Ile ALa Tyr Glu Glu Ile Phe Val Lys Asn Met. 12. Værtscelle transficeret eller transformeret med en ekspressionsvektor ifølge krav 9, 10 eller 11. 13. Fremgangsmåde til fremstilling af et vævsfaktorinhibitor-polypeptid, hvilken fremgangsmåde omfatter, at man: (a) dyrker en transficeret eller transformeret værtscelle ifølge krav 12 under egnede næringsbetingelser på en måde, som tillader ekspression af det nævnte polypeptid; og (b) isolerer det nævnte polypeptid.
DK200200302A 1987-11-23 2002-02-27 Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........ DK175430B1 (da)

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DK200200302A DK175430B1 (da) 1987-11-23 2002-02-27 Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........

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US07/123,753 US4966852A (en) 1987-07-23 1987-11-23 DNA clone of human tissue factor inhibitor
DK200001612A DK174754B1 (da) 1987-11-23 2000-10-27 Humane vævsfaktorinhibitor (TFI), antistof med bindingsspecificitet herfor, fremgangsmåde til anvendelse af en TFI-peptid-kolonne til at oprense et antistof og fremgangsmåder til detektion af TFI eller et polypeptid omfattende....
DK200200302A DK175430B1 (da) 1987-11-23 2002-02-27 Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........

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DK200200301A DK175431B1 (da) 1987-11-23 2002-02-27 Antistoffer som binder fragmenter af vævsfaktorinhibitor (TFI), sammensætninger omfattende disse antistoffer, og fremgangsmåde til anvendelse af en immunoaffinitetsmatrix omfattende et sådant antistof til at oprense et polypeptid fra et biologisk fluidum
DK200200302A DK175430B1 (da) 1987-11-23 2002-02-27 Rekombinante polypeptider omfattende aminosyresekvensen af moden vævsfaktorinhibitor (TFI), polypeptidfragmenter deraf, ekspressionsvektorer der koder for disse, værtsceller transformeret dermed, og fremgangsmåde til fremstilling af et ........

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