DK167852B1 - Fremgangsmaade til inaktivering af rekombinant dna - Google Patents
Fremgangsmaade til inaktivering af rekombinant dna Download PDFInfo
- Publication number
- DK167852B1 DK167852B1 DK268589A DK268589A DK167852B1 DK 167852 B1 DK167852 B1 DK 167852B1 DK 268589 A DK268589 A DK 268589A DK 268589 A DK268589 A DK 268589A DK 167852 B1 DK167852 B1 DK 167852B1
- Authority
- DK
- Denmark
- Prior art keywords
- dna
- peracetic acid
- incubation
- per
- process according
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 20
- 108020004511 Recombinant DNA Proteins 0.000 title claims abstract description 9
- 230000000415 inactivating effect Effects 0.000 title claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 9
- 239000002028 Biomass Substances 0.000 claims abstract description 7
- 230000004071 biological effect Effects 0.000 claims abstract description 5
- 238000010438 heat treatment Methods 0.000 claims abstract description 5
- SCKXCAADGDQQCS-UHFFFAOYSA-N Performic acid Chemical compound OOC=O SCKXCAADGDQQCS-UHFFFAOYSA-N 0.000 claims abstract description 4
- 125000004432 carbon atom Chemical group C* 0.000 claims abstract description 4
- 150000003839 salts Chemical class 0.000 claims abstract 2
- KFSLWBXXFJQRDL-UHFFFAOYSA-N Peracetic acid Chemical compound CC(=O)OO KFSLWBXXFJQRDL-UHFFFAOYSA-N 0.000 claims description 66
- 108020004414 DNA Proteins 0.000 claims description 30
- 238000011534 incubation Methods 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 15
- 239000013612 plasmid Substances 0.000 claims description 14
- 239000003513 alkali Substances 0.000 claims description 7
- 239000002585 base Substances 0.000 claims description 6
- 150000002978 peroxides Chemical class 0.000 claims description 5
- 239000007984 Tris EDTA buffer Substances 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 238000004113 cell culture Methods 0.000 claims 2
- 229920001817 Agar Polymers 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 239000008272 agar Substances 0.000 claims 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 claims 1
- 229960000723 ampicillin Drugs 0.000 claims 1
- 230000001580 bacterial effect Effects 0.000 claims 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims 1
- 239000012895 dilution Substances 0.000 claims 1
- 238000010790 dilution Methods 0.000 claims 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- 150000004973 alkali metal peroxides Chemical class 0.000 abstract 1
- 150000001340 alkali metals Chemical class 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 description 10
- 239000012634 fragment Substances 0.000 description 10
- 244000005700 microbiome Species 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 7
- 238000000246 agarose gel electrophoresis Methods 0.000 description 5
- 239000011734 sodium Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 4
- 235000014347 soups Nutrition 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 210000003527 eukaryotic cell Anatomy 0.000 description 3
- 238000013467 fragmentation Methods 0.000 description 3
- 238000006062 fragmentation reaction Methods 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 230000002779 inactivation Effects 0.000 description 3
- 239000012723 sample buffer Substances 0.000 description 3
- 239000010802 sludge Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 150000003863 ammonium salts Chemical class 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- CZPZWMPYEINMCF-UHFFFAOYSA-N propaneperoxoic acid Chemical compound CCC(=O)OO CZPZWMPYEINMCF-UHFFFAOYSA-N 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- PFUVRDFDKPNGAV-UHFFFAOYSA-N sodium peroxide Chemical compound [Na+].[Na+].[O-][O-] PFUVRDFDKPNGAV-UHFFFAOYSA-N 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances
- A61L2/0088—Liquid substances
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873733921 DE3733921A1 (de) | 1987-10-07 | 1987-10-07 | Verfahren zur inaktivierung rekombinanter dna |
| DE3733921 | 1987-10-07 | ||
| PCT/EP1988/000592 WO1989003226A1 (fr) | 1987-10-07 | 1988-07-04 | Procede pour inactiver de l'adn recombinant |
| EP8800592 | 1988-07-04 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK268589A DK268589A (da) | 1989-06-01 |
| DK268589D0 DK268589D0 (da) | 1989-06-01 |
| DK167852B1 true DK167852B1 (da) | 1993-12-27 |
Family
ID=6337823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK268589A DK167852B1 (da) | 1987-10-07 | 1989-06-01 | Fremgangsmaade til inaktivering af rekombinant dna |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5118603A (fr) |
| EP (1) | EP0334904B1 (fr) |
| JP (1) | JPH0642831B2 (fr) |
| AT (1) | ATE76585T1 (fr) |
| AU (1) | AU598945B2 (fr) |
| DE (2) | DE3733921A1 (fr) |
| DK (1) | DK167852B1 (fr) |
| FI (1) | FI892769A0 (fr) |
| WO (1) | WO1989003226A1 (fr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4015832A1 (de) * | 1990-05-17 | 1991-11-21 | Thomae Gmbh Dr K | Verfahren zur inaktivierung der biologischen aktivitaet von dna |
| GB9222561D0 (en) * | 1992-10-27 | 1992-12-09 | Ici Plc | Macromolecular degradation |
| US9804069B2 (en) * | 2012-12-19 | 2017-10-31 | Dnae Group Holdings Limited | Methods for degrading nucleic acid |
| WO2014151748A1 (fr) | 2013-03-15 | 2014-09-25 | Dsm Ip Assets B.V. | Utilisation de nucléases thermophiles pour la dégradation d'acides nucléiques |
| JP6057175B2 (ja) * | 2013-04-23 | 2017-01-11 | 国立研究開発法人産業技術総合研究所 | 非脱気式の高温高圧水蒸気処理装置を用いて汚染を漏らさず高速にｄｎａを分解除去する方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US798013A (en) * | 1904-02-23 | 1905-08-22 | Alphonso M Clover | Antiseptic compound. |
| US2058315A (en) * | 1930-11-14 | 1936-10-20 | Buffalo Electro Chemical Co In | Organic persulphonic compounds and process of preparing same |
| US2536008A (en) * | 1949-02-21 | 1950-12-26 | Shell Dev | Peroxy-substituted persulfonates |
| US2957931A (en) * | 1949-07-28 | 1960-10-25 | Socony Mobil Oil Co Inc | Synthesis of compounds having a carbonphosphorus linkage |
| US2802025A (en) * | 1952-11-12 | 1957-08-06 | Degussa | Process for the production of lower carboxylic peracids |
| GB1568358A (en) * | 1975-11-18 | 1980-05-29 | Interox Chemicals Ltd | Aromatic peroxyacids and their use in bleaching processes |
| JPS61152275A (ja) * | 1984-12-27 | 1986-07-10 | Suntory Ltd | 組換え微生物の殺菌法 |
-
1987
- 1987-10-07 DE DE19873733921 patent/DE3733921A1/de not_active Withdrawn
-
1988
- 1988-07-04 WO PCT/EP1988/000592 patent/WO1989003226A1/fr active IP Right Grant
- 1988-07-04 AT AT88905801T patent/ATE76585T1/de not_active IP Right Cessation
- 1988-07-04 US US07/363,887 patent/US5118603A/en not_active Expired - Fee Related
- 1988-07-04 JP JP63505715A patent/JPH0642831B2/ja not_active Expired - Lifetime
- 1988-07-04 EP EP88905801A patent/EP0334904B1/fr not_active Expired - Lifetime
- 1988-07-04 DE DE8888905801T patent/DE3871519D1/de not_active Expired - Lifetime
- 1988-07-04 AU AU19888/88A patent/AU598945B2/en not_active Ceased
-
1989
- 1989-06-01 DK DK268589A patent/DK167852B1/da not_active IP Right Cessation
- 1989-06-06 FI FI892769A patent/FI892769A0/fi not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| DE3733921A1 (de) | 1989-04-20 |
| JPH01503197A (ja) | 1989-11-02 |
| AU1988888A (en) | 1989-05-02 |
| US5118603A (en) | 1992-06-02 |
| DE3871519D1 (de) | 1992-07-02 |
| AU598945B2 (en) | 1990-07-05 |
| EP0334904B1 (fr) | 1992-05-27 |
| ATE76585T1 (de) | 1992-06-15 |
| JPH0642831B2 (ja) | 1994-06-08 |
| FI892769A (fi) | 1989-06-06 |
| FI892769A0 (fi) | 1989-06-06 |
| DK268589A (da) | 1989-06-01 |
| WO1989003226A1 (fr) | 1989-04-20 |
| DK268589D0 (da) | 1989-06-01 |
| EP0334904A1 (fr) | 1989-10-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B1 | Patent granted (law 1993) | ||
| PBP | Patent lapsed |