DK1654360T3 - Amplificering fremgangsmåde - Google Patents
Amplificering fremgangsmådeInfo
- Publication number
- DK1654360T3 DK1654360T3 DK04743697T DK04743697T DK1654360T3 DK 1654360 T3 DK1654360 T3 DK 1654360T3 DK 04743697 T DK04743697 T DK 04743697T DK 04743697 T DK04743697 T DK 04743697T DK 1654360 T3 DK1654360 T3 DK 1654360T3
- Authority
- DK
- Denmark
- Prior art keywords
- amplification procedure
- amplification
- procedure
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0319332.3A GB0319332D0 (en) | 2003-08-16 | 2003-08-16 | Amplification |
PCT/GB2004/003486 WO2005019452A1 (en) | 2003-08-16 | 2004-08-12 | Amplification method |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1654360T3 true DK1654360T3 (da) | 2008-02-25 |
Family
ID=28052667
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK04743697T DK1654360T3 (da) | 2003-08-16 | 2004-08-12 | Amplificering fremgangsmåde |
Country Status (8)
Country | Link |
---|---|
US (1) | US20070105090A1 (da) |
EP (1) | EP1654360B1 (da) |
JP (1) | JP2007502610A (da) |
DE (1) | DE602004010273T2 (da) |
DK (1) | DK1654360T3 (da) |
ES (1) | ES2295886T3 (da) |
GB (1) | GB0319332D0 (da) |
WO (1) | WO2005019452A1 (da) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101606929B1 (ko) | 2008-04-30 | 2016-03-29 | 포항공과대학교 산학협력단 | 모세관 전기영동에 의한 다중 mRNA 또는 16S rRNA분석을 위한 주형 변형방법 및 이를 이용한 미생물검출방법 |
EP3998346A1 (en) * | 2009-03-30 | 2022-05-18 | Illumina, Inc. | Gene expression analysis in single cells |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
ES2663234T3 (es) | 2012-02-27 | 2018-04-11 | Cellular Research, Inc | Composiciones y kits para recuento molecular |
EP2912197B1 (en) * | 2012-10-24 | 2019-08-07 | Takara Bio USA, Inc. | Template switch-based methods for producing a product nucleic acid |
DK3611262T3 (da) * | 2013-03-15 | 2021-01-25 | Lineage Biosciences Inc | Fremgangsmåder til sekvensering af immunrepertoire |
EP3842542A1 (en) | 2013-08-28 | 2021-06-30 | Becton, Dickinson and Company | Massively parallel single cell analysis |
JP6602294B2 (ja) | 2013-10-17 | 2019-11-06 | タカラ バイオ ユーエスエー, インコーポレイテッド | アダプタを核酸に追加する方法、及びこの方法を実行するための組成物 |
US9719136B2 (en) | 2013-12-17 | 2017-08-01 | Takara Bio Usa, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
CN107250379B (zh) | 2015-02-19 | 2021-12-28 | 贝克顿迪金森公司 | 结合蛋白质组信息和基因组信息的高通量单细胞分析 |
WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
EP3286326A1 (en) | 2015-04-23 | 2018-02-28 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
US11124823B2 (en) | 2015-06-01 | 2021-09-21 | Becton, Dickinson And Company | Methods for RNA quantification |
CN108026524A (zh) | 2015-09-11 | 2018-05-11 | 赛卢拉研究公司 | 用于核酸文库标准化的方法和组合物 |
CN109072288A (zh) | 2016-05-02 | 2018-12-21 | 赛卢拉研究公司 | 精确的分子条形编码 |
US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
EP3465502B1 (en) | 2016-05-26 | 2024-04-10 | Becton, Dickinson and Company | Molecular label counting adjustment methods |
US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
SG11201901733PA (en) | 2016-09-26 | 2019-04-29 | Cellular Res Inc | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
WO2018132610A1 (en) | 2017-01-13 | 2018-07-19 | Cellular Research, Inc. | Hydrophilic coating of fluidic channels |
EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
AU2018281745B2 (en) | 2017-06-05 | 2022-05-19 | Becton, Dickinson And Company | Sample indexing for single cells |
CN111492068A (zh) | 2017-12-19 | 2020-08-04 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
ES2945191T3 (es) | 2018-05-03 | 2023-06-29 | Becton Dickinson Co | Análisis de muestras multiómicas de alto rendimiento |
US11365409B2 (en) | 2018-05-03 | 2022-06-21 | Becton, Dickinson And Company | Molecular barcoding on opposite transcript ends |
CN112805389A (zh) | 2018-10-01 | 2021-05-14 | 贝克顿迪金森公司 | 确定5’转录物序列 |
WO2020097315A1 (en) | 2018-11-08 | 2020-05-14 | Cellular Research, Inc. | Whole transcriptome analysis of single cells using random priming |
WO2020123384A1 (en) | 2018-12-13 | 2020-06-18 | Cellular Research, Inc. | Selective extension in single cell whole transcriptome analysis |
WO2020150356A1 (en) | 2019-01-16 | 2020-07-23 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
EP3914728B1 (en) | 2019-01-23 | 2023-04-05 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
WO2020214642A1 (en) | 2019-04-19 | 2020-10-22 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
EP3972611A4 (en) * | 2019-05-21 | 2023-06-14 | Academia Sinica | METHODS FOR AMPLIFICATION AND DETECTION OF RIBONUCLEAN ACID (RNA) FRAGMENTS |
CN110241459A (zh) * | 2019-05-31 | 2019-09-17 | 南方医科大学南方医院 | 一种甄别样品间与独立样品自身交叉反应的免疫组库方法 |
EP4004231A1 (en) | 2019-07-22 | 2022-06-01 | Becton, Dickinson and Company | Single cell chromatin immunoprecipitation sequencing assay |
CN112501249B (zh) * | 2019-09-16 | 2024-01-26 | 深圳市真迈生物科技有限公司 | Rna文库的制备方法、测序方法和试剂盒 |
CN114729350A (zh) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | 使用随机引发获得用于免疫组库测序的全长v(d)j信息 |
WO2021146207A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and rna |
CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司(Us) | 用于免疫组库谱分析的引物 |
US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
AU2001251069A1 (en) * | 2000-03-28 | 2001-10-08 | The Government of the United States of America, as represented by the Secretary, Department of Health and Human Services, The NationalInstitutes Of Health | Gene profiling arrays |
US6465219B1 (en) * | 2000-08-07 | 2002-10-15 | Genemed Biotechnologies, Inc. | Polynucleotide pools enriched in either high-abundance or low-abundance sequences |
US20040023271A1 (en) * | 2002-03-29 | 2004-02-05 | Nurith Kurn | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
-
2003
- 2003-08-16 GB GBGB0319332.3A patent/GB0319332D0/en not_active Ceased
-
2004
- 2004-08-12 DK DK04743697T patent/DK1654360T3/da active
- 2004-08-12 EP EP04743697A patent/EP1654360B1/en not_active Not-in-force
- 2004-08-12 WO PCT/GB2004/003486 patent/WO2005019452A1/en active IP Right Grant
- 2004-08-12 ES ES04743697T patent/ES2295886T3/es active Active
- 2004-08-12 JP JP2006523675A patent/JP2007502610A/ja active Pending
- 2004-08-12 DE DE602004010273T patent/DE602004010273T2/de not_active Expired - Fee Related
- 2004-11-16 US US10/568,432 patent/US20070105090A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DE602004010273D1 (de) | 2008-01-03 |
WO2005019452A1 (en) | 2005-03-03 |
ES2295886T3 (es) | 2008-04-16 |
EP1654360A1 (en) | 2006-05-10 |
GB0319332D0 (en) | 2003-09-17 |
DE602004010273T2 (de) | 2008-09-25 |
EP1654360B1 (en) | 2007-11-21 |
US20070105090A1 (en) | 2007-05-10 |
JP2007502610A (ja) | 2007-02-15 |
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