DK159406B - PROCEDURE FOR QUICK DETERMINATION OF THE BLOOD LOWER IN VENE BLOOD - Google Patents
PROCEDURE FOR QUICK DETERMINATION OF THE BLOOD LOWER IN VENE BLOOD Download PDFInfo
- Publication number
- DK159406B DK159406B DK021685A DK21685A DK159406B DK 159406 B DK159406 B DK 159406B DK 021685 A DK021685 A DK 021685A DK 21685 A DK21685 A DK 21685A DK 159406 B DK159406 B DK 159406B
- Authority
- DK
- Denmark
- Prior art keywords
- blood
- lowering
- tube
- vene
- procedure
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 21
- 210000004369 blood Anatomy 0.000 title claims description 11
- 239000008280 blood Substances 0.000 title claims description 11
- 238000012360 testing method Methods 0.000 claims description 4
- 238000007792 addition Methods 0.000 claims description 2
- 210000003743 erythrocyte Anatomy 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000005499 meniscus Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000009666 routine test Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 230000008320 venous blood flow Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/05—Investigating sedimentation of particle suspensions in blood
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
DK 159406BDK 159406B
iin
En af de mest benyttede laboratorieprøver i den almene lægepraksis er bestemmelse af sænkningen i veneblod.One of the most commonly used laboratory tests in general medical practice is the determination of the reduction in vein blood.
Den traditionelle metode ifølge Westergren er forbundet med den praktiske ulempe, at det tager 1 time før resultatet foreligger; meget 5 ofte vil konsultationen da allerede være afsluttet.The traditional method according to Westergren is associated with the practical disadvantage that it takes 1 hour before the result is available; very often the consultation will already be completed.
Der har været gjort mange forsøg på at modificere denne metode for at opnå en sænkningsbestemmelse på kortere tid. Imidlertid har metoder med opvarmning af sænkningsblodet eller kemiske tilsætninger ikke været vellykkede.Many attempts have been made to modify this method to obtain a lowering determination in a shorter time. However, methods of heating the lowering blood or chemical additives have not been successful.
10 Det har imidlertid vist sig, at en skråstilling af sænkningsbestemmelsesrøret giver en betydeligt forøget hastighed, og dette har været grundlaget for flere forsøg på såkaldte hurtigmetoder.10 However, it has been found that an inclination of the lowering determination tube gives a significantly increased speed, and this has been the basis for several attempts at so-called rapid methods.
Årsagen til den forøgede hastighed er, at rørets skråstilling bevirker en hurtigere "pengerulledannelse" og aggregering af erytrocytter-15 ne mod glasvæggen i pipetten, idet opadstigende plasma yder mindre modstand, og sedimenteringshastigheden forøges.The reason for the increased velocity is that the slope of the tube causes a faster "money roll" formation and aggregation of the erythrocytes against the glass wall of the pipette, as the rising plasma provides less resistance and the sedimentation rate is increased.
Den mest anvendte hurtigmetode medfører, at sænkningsrøret vippes 60° fra verti kålplanet, og det er angivet, at en aflæsning af sænkningen efter henholdsvis 7 og 10 minutter skulle give et pålideligt udtryk for 20 den almene vertikale sænkning aflæst efter henholdsvis 1 og 2 timer.The most commonly used fast method causes the lowering tube to tilt 60 ° from the vertical cabbage plane, and it is stated that a reading of the lowering after 7 and 10 minutes, respectively, should give a reliable expression of the general vertical lowering read after 1 and 2 hours respectively.
Der er endvidere gjort forsøg med andre hældningsvinkler.Furthermore, experiments have been made with other inclination angles.
Der er blevet foretaget nærmere undersøgelser med hensyn til disse metoders pålidelighed, og der skal her henvises til en artikel af Steinar Westin og Astrid Helland, "Senkningsmåling i almenpraksis" samt 25 en artikel af Jon Block og Martin Holte "Hurtigsenkning" begge artikler fra "Tidsskrift for den norske Legeforening" nr. 29, 1976, henholdsvis side 1551-1553 og 1553 -1555.Further studies have been conducted on the reliability of these methods, and reference should be made here to an article by Steinar Westin and Astrid Helland, "Lowering Measurement in General Practice" and 25 an article by Jon Block and Martin Holte "Quick Lowering" both articles from " Journal of the Norwegian Medical Association "No. 29, 1976, pages 1551-1553 and 1553 -1555 respectively.
I begge artikler konkluderes det, at den såkaldte skråmetode kun bør benyttes i undtagelsestilfælde (Westin og Helland), samt at konse-30 kvent brug af kun hurtigsænkning ikke er anbefalelsesværdig (Block og Holte).In both articles it is concluded that the so-called slanting method should only be used in exceptional cases (Westin and Helland), and that consistent use of only rapid lowering is not advisable (Block and Holte).
Ud over iagttagelserne og anmærkningerne ifølge disse to artikler har det vist sig, at Westergrens 60 minutters metode heller ikke udviser den gode og sikre reproducerbarhed, som er ønskelig.In addition to the observations and remarks made by these two articles, it has been found that Westergren's 60 minute method does not exhibit the good and safe reproducibility that is desirable.
35 Årsagen til dette kan være en blandingseffekt i blodet som suges op i pipetten, hvor hurtigt eller langsomt blodet suges op og pipettens vo-lumennøjagtighed.The reason for this may be a mixing effect in the blood which is sucked up into the pipette, how quickly or slowly the blood is sucked up and the volume accuracy of the pipette.
Nogle af de problemer, som ifølge de ovennævnte artiklers forfat- DK 159406 B z tere opstår ved, og som kan være årsagen til, at de tidligere hurtigsænkningsmetoder ikke er helt anbefalelsesværdige, er den "hale" af røde blodlegemer, som dannes, når sænkningsrøret står i skråstilling.Some of the problems encountered by the authors of the above-mentioned articles, and which may be the reason why the previous quick-lowering methods are not wholly recommendable, are the "tail" of red blood cells that form when the lowering tube stands in an oblique position.
Den foreliggende opfindelse har til hensigt at afhjælpe ulemperne 5 ved den kendte teknik og således tilvejebringe en hurtig, pålidelig og reproducerbar metode til måling af blodsænkningen i veneblod.The present invention aims to alleviate the disadvantages of the prior art and thus to provide a fast, reliable and reproducible method for measuring venous blood flow.
Dette opnås ved en fremgangsmåde, hvorved blodet efter de nødvendige tilsætninger hensættes i et til sænkningsbestemmelse egnet rør, hvor bestemmelsesrøret ved bestemmelsens påbegyndelse anbringes i en 10 vinkel i forhold til verti kali i nien, og fremgangsmåden er karakteriseret ved, at røret, eventuelt efter at have stået stille i skråstilling, dernæst samtidig gives en såvel om sin egen akse roterende som længdeaksen oprettende bevægelse, hvorefter sænkningen aflæses umiddelbart efter, at røret har nået vertikal stilling, eller efter, at det i et tidsrum har 15 stået stille i vertikal stilling.This is achieved by a method whereby, after the necessary additions, the blood is deposited in a tube suitable for lowering, where at the beginning of the test tube the test tube is placed at an angle to the vertical in the nine, and the method is characterized in that the tube, possibly after have been stationary in an oblique position, then at the same time a motion is created both about its own axis rotating and the longitudinal axis, after which the lowering is read immediately after the pipe has reached a vertical position or after it has been stationary in a vertical position for a period of time.
Udførte undersøgelser antyder, at denne metode giver mere konsistente og reproducerbare resultater.Studies conducted suggest that this method yields more consistent and reproducible results.
Opfindelsen beskrives nærmere i det følgende under henvisning til tegningen, hvor 20 Fig. 1 viser en klassisk skråsti11ingssituation,The invention will now be described in more detail with reference to the accompanying drawings, in which 1 shows a classic inclined position,
Fig. 2 viser en udvikling af denne teknik, ogFIG. 2 shows a development of this technique, and
Fig. 3 og 4 illustrerer opfindelsen.FIG. 3 and 4 illustrate the invention.
I figurerne har samme elementer eller elementer, som opfylder samme funktion, fået samme henvisningstal.In the figures, the same elements or elements that fulfill the same function have the same reference numerals.
25 Fig. 1 viser en klassisk skråsti11ingsmetode, efter at bestemmelsen har forløbet i et stykke tid.FIG. 1 shows a classic slanting method after the determination has been going on for a while.
Citratblodet, som er fyldt i bestemmelsesrøret 6, er begyndt at skille sig ad i en fraktion 4 med øget erytrocytkoncentration og derover liggende plasma 3. Med 5 angives den menisk, som dannes i bestemmelses-30 røret.The citrate blood, which is filled in the assay tube 6, has begun to separate into a fraction 4 with increased erythrocyte concentration and overlying plasma 3. By 5, the meniscus formed in the assay tube is indicated.
Det ses, at der ved erytrocytafsætningen dannes en hale 2', som til dels vil forstyrre bestemmelsen.It is seen that at the erythrocyte deposition, a tail 2 'is formed, which will partly interfere with the determination.
Bestemmelsesrøret 6 er i bunden forsynet med en gummiprop 7 og hviler på et egnet stativ 8.The test tube 6 is provided at the bottom with a rubber plug 7 and rests on a suitable stand 8.
35 I fig. 2 vises en videreudvikling af den såkaldte "passive" skråsti 11 ingsmetode, hvor bestemmelsesrøret hviler på stativet 8, men ydermere er forsynet med et roterende element 9, som er forbundet med røret 6 ved hjælp af en forbindelse 9', idet rotationselementet drejer omkring 335 In FIG. 2, a further development of the so-called "passive" inclination path 11 method is shown, in which the determining tube rests on the frame 8, but is further provided with a rotating element 9 which is connected to the tube 6 by means of a connection 9 ', the rotating element rotating about 3
DK 159406 BDK 159406 B
et dertil egnet stativ.a suitable stand for this purpose.
Ved denne i og for sig kendte drejning af bestemmelsesrøret opnår man, at den hale, som er vist i fig. 1 med 2', undgås.By this known rotation of the assay tube one obtains that the tail shown in FIG. 1 by 2 'is avoided.
Der har imidlertid vist sig, at denne fremgangsmåde heller ikke gi-5 ver den ønskede grad af reproducerbarhed og nøjagtighed, og ansøgeren er kommet frem til en metode som ovenfor angivet.However, it has been found that this method also does not provide the desired degree of reproducibility and accuracy, and the applicant has devised a method as set forth above.
Denne metode beskrives under henvisning til fig. 3 og 4.This method is described with reference to FIG. 3 and 4.
I fig. 4 hviler røret i et leje, som dannes af to huller i ører 8', som igen er fastgjort til en aksel, der står i forbindelse med for ek-10 sempel et tidsur. Røret er forsynet med en ring 8", for at det ikke skal glide.In FIG. 4, the tube rests in a bearing formed by two holes in the ears 8 ', which in turn are attached to a shaft which is connected to, for example, a timer. The tube is provided with a ring 8 "so that it does not slip.
Bestemmelsen gennemføres på følgende måde: røret hensættes først i en skrå stilling og gives, eventuelt efter at have stået stille i skråstilling, en samtidig akseoprettende bevægelse omkring drejeaksen 8 og 15 samtidig en om sin egen akse roterende bevægelse ved hjælp af et dertil egnet hjul 10, som bringes til rotation ved kontakt med et egnet anlæg 11.The determination is carried out as follows: the pipe is first placed in an oblique position and, optionally after standing in an oblique position, a simultaneous axis-creating movement about the pivot axis 8 and 15 is simultaneously given a rotation about its own axis by means of a suitable wheel 10. which is brought into rotation by contact with a suitable plant 11.
Teorien, som ligger til grund for den foreliggende opfindelse, er, at halen, der opstår som antydet i fig. 1, skyldes en for hurtig sedi-20 mentering. Denne lidt for hurtige sedimentering kan afhjælpes ved at dreje røret, som antydet i forbindelse med fig. 2, men de bedste resultater opnås, når røret ud over drejebevægelsen gives en samtidig bevægelse fra skråstilling til vertikal stilling.The theory underlying the present invention is that the tail arising as indicated in FIG. 1, is due to excessive sedimentation. This slightly too rapid sedimentation can be remedied by turning the tube, as indicated in connection with FIG. 2, but the best results are obtained when, in addition to the pivotal movement, the tube is given a simultaneous movement from the slant to the vertical position.
Det er klart, at andre midler end de i fig. 3 og 4 viste vil kunne 25 anvendes, og at opretningstiden og omdrejningshastigheden kan varieres inden for vide grænser: disse værdier kan bestemmes ved rutineforsøg af den enkelte læge, alt efter hvilket apparatur han benytter.It is clear that other means than those of FIG. 3 and 4 can be used and that the set-up time and the speed of rotation can be varied within wide limits: these values can be determined by routine tests by the individual doctor, according to the apparatus he uses.
Claims (1)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NO840179 | 1984-01-18 | ||
| NO840179A NO153508C (en) | 1984-01-18 | 1984-01-18 | QUICK METHOD FOR DETERMINING THE REDUCTION IN VENE BLOOD. |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| DK21685D0 DK21685D0 (en) | 1985-01-17 |
| DK21685A DK21685A (en) | 1985-07-19 |
| DK159406B true DK159406B (en) | 1990-10-08 |
| DK159406C DK159406C (en) | 1991-03-04 |
Family
ID=19887442
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK021685A DK159406C (en) | 1984-01-18 | 1985-01-17 | PROCEDURE FOR QUICK DETERMINATION OF THE BLOOD LOWER IN VENE BLOOD |
Country Status (7)
| Country | Link |
|---|---|
| DE (1) | DE3500565A1 (en) |
| DK (1) | DK159406C (en) |
| FI (1) | FI78179C (en) |
| GB (1) | GB2153072B (en) |
| IT (1) | IT1201210B (en) |
| NO (1) | NO153508C (en) |
| SE (1) | SE8500199L (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE467508B (en) * | 1990-11-29 | 1992-07-27 | Hemocue Ab | DEVICE FOR QUICK PERFORMANCE OF BLOOD RECONCILIATION REACTION |
| ES2151939T3 (en) * | 1995-07-21 | 2001-01-16 | Becton Dickinson Co | METHOD AND APPLIANCE TO DETERMINE THE RATE OF SEDIMENTATION OF Erythrocytes. |
| DE69515858T2 (en) * | 1995-07-21 | 2000-07-20 | Becton Dickinson And Co., Franklin Lakes | Sample tube for blood sedimentation determination and a detergent for use therein |
| US5914272A (en) * | 1996-06-19 | 1999-06-22 | Becton Dickinson And Company | Test method for determining the erythrocyte sedimentation rate and a surfactant for use therein |
| US6974701B2 (en) | 2003-03-21 | 2005-12-13 | Hemovations, Llc | Erythrocyte sedimentation rate (ESR) test measurement instrument of unitary design and method of using the same |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE7724794U1 (en) * | 1979-02-08 | Ernst Leitz Wetzlar Gmbh, 6330 Wetzlar | Device for determining the rate of sedimentation | |
| DE894925C (en) * | 1949-11-01 | 1955-03-07 | Walter Stein Dr Med | Apparatus for measuring the rate of sedimentation |
-
1984
- 1984-01-18 NO NO840179A patent/NO153508C/en unknown
-
1985
- 1985-01-10 DE DE19853500565 patent/DE3500565A1/en active Granted
- 1985-01-11 GB GB08500749A patent/GB2153072B/en not_active Expired
- 1985-01-16 SE SE8500199A patent/SE8500199L/en unknown
- 1985-01-16 FI FI850185A patent/FI78179C/en not_active IP Right Cessation
- 1985-01-17 DK DK021685A patent/DK159406C/en not_active IP Right Cessation
- 1985-01-17 IT IT09310/85A patent/IT1201210B/en active
Also Published As
| Publication number | Publication date |
|---|---|
| SE8500199L (en) | 1985-07-19 |
| DK21685D0 (en) | 1985-01-17 |
| GB8500749D0 (en) | 1985-02-13 |
| GB2153072A (en) | 1985-08-14 |
| DE3500565C2 (en) | 1988-03-24 |
| FI78179C (en) | 1989-06-12 |
| FI850185L (en) | 1985-07-19 |
| FI78179B (en) | 1989-02-28 |
| DK21685A (en) | 1985-07-19 |
| IT8509310A0 (en) | 1985-01-17 |
| DK159406C (en) | 1991-03-04 |
| SE8500199D0 (en) | 1985-01-16 |
| IT1201210B (en) | 1989-01-27 |
| NO840179L (en) | 1985-07-19 |
| FI850185A0 (en) | 1985-01-16 |
| NO153508C (en) | 1986-04-02 |
| GB2153072B (en) | 1987-08-05 |
| DE3500565A1 (en) | 1985-07-25 |
| NO153508B (en) | 1985-12-23 |
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| Date | Code | Title | Description |
|---|---|---|---|
| PBP | Patent lapsed |