DK1442134T3 - Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner - Google Patents

Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner

Info

Publication number
DK1442134T3
DK1442134T3 DK02774994T DK02774994T DK1442134T3 DK 1442134 T3 DK1442134 T3 DK 1442134T3 DK 02774994 T DK02774994 T DK 02774994T DK 02774994 T DK02774994 T DK 02774994T DK 1442134 T3 DK1442134 T3 DK 1442134T3
Authority
DK
Denmark
Prior art keywords
soluble protein
protein domains
fragments
library
identification
Prior art date
Application number
DK02774994T
Other languages
Danish (da)
English (en)
Inventor
Mark Mcalister
Renos Savva
Laurence Pearl
Chrisostomos Prodromou
Paul Driscoll
Original Assignee
Domainex Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Domainex Ltd filed Critical Domainex Ltd
Application granted granted Critical
Publication of DK1442134T3 publication Critical patent/DK1442134T3/da

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK02774994T 2001-11-08 2002-11-08 Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner DK1442134T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0126887.9A GB0126887D0 (en) 2001-11-08 2001-11-08 Method for producing and identifying soluble protein domains
PCT/GB2002/005075 WO2003040391A2 (en) 2001-11-08 2002-11-08 Method for producing and identifying soluble protein domains

Publications (1)

Publication Number Publication Date
DK1442134T3 true DK1442134T3 (da) 2009-01-12

Family

ID=9925436

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02774994T DK1442134T3 (da) 2001-11-08 2002-11-08 Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner

Country Status (10)

Country Link
US (2) US8852866B2 (https=)
EP (1) EP1442134B1 (https=)
JP (1) JP4303112B2 (https=)
AT (1) ATE408029T1 (https=)
AU (1) AU2002341204B2 (https=)
CA (1) CA2465377C (https=)
DE (1) DE60228867D1 (https=)
DK (1) DK1442134T3 (https=)
GB (1) GB0126887D0 (https=)
WO (1) WO2003040391A2 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE452992T1 (de) * 2004-02-04 2010-01-15 Qiagen North American Holdings Zusammensetzungen auf dutp-basis zurverringerung der primeraggregatbildung während der nukleinsäureamplifikation
EP3257949A1 (en) 2005-06-15 2017-12-20 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
WO2007089591A2 (en) * 2006-01-27 2007-08-09 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for high throughput screening of pharmacological chaperones
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
EP2977455B1 (en) * 2009-06-15 2020-04-15 Complete Genomics, Inc. Method for long fragment read sequencing
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
JP2011147403A (ja) * 2010-01-22 2011-08-04 Hitachi High-Technologies Corp 細菌検査装置及び細菌検査方法
KR101629773B1 (ko) * 2014-07-16 2016-06-13 한국과학기술원 약물 펩타이드 후보 선별 장치 및 이를 이용한 약물 펩타이드 후보 선별 방법
DK3294934T3 (da) * 2015-07-03 2020-03-23 Hunan Zonsen Peplib Biotech Co Ltd Fremgangsmåde til opbygning af peptidbibliotek og relaterede vektorer
JP7013039B2 (ja) * 2017-04-26 2022-01-31 フーナン ゾンセン ペプリブ バイオテック カンパニー リミテッド ペプチドライブラリーの構築方法
CA3238393A1 (en) * 2021-11-17 2023-05-25 Nerd Bio Llc Systems and methods for real-time cellular drug-target engagement

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU638762B2 (en) * 1989-10-05 1993-07-08 Optein Inc Cell-free synthesis and isolation of novel genes and polypeptides
US6117679A (en) * 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5805923A (en) * 1995-05-26 1998-09-08 Sony Corporation Configurable power management system having a clock stabilization filter that can be enabled or bypassed depending upon whether a crystal or can oscillator is used
US6190865B1 (en) * 1995-09-27 2001-02-20 Epicentre Technologies Corporation Method for characterizing nucleic acid molecules
DE19545320A1 (de) * 1995-12-05 1997-06-12 Boehringer Mannheim Gmbh Thermolabile Uracil-DNA-Glykosylase, Verfahren zu deren Herstellung und Verwendung zur Entfernung von Uracil aus DNA
US7175982B1 (en) 1998-04-22 2007-02-13 Enterprise Ireland (T/A BioResearch Ireland) Method for the characterization of nucleic acid molecules involving generation of extendible upstream DNA fragments resulting from the cleavage of nucleic acid at an abasic site
ATE320486T1 (de) * 1999-05-05 2006-04-15 Phylogica Ltd Isolierung von biologischen modulatoren aus bibiotheken mit biologisch vielfältigen genfragmenten
IL150291A0 (en) * 2000-01-11 2002-12-01 Maxygen Inc Integrated systems and methods for diversity generation and screening
AU2001243670A1 (en) * 2000-03-20 2001-10-03 Maxygen, Inc. Method for generating recombinant dna molecules in complex mixtures
US7785828B1 (en) * 2003-02-06 2010-08-31 Pioneer Hi-Bred International, Inc. Production of antimicrobial proteins in fusion proteins

Also Published As

Publication number Publication date
DE60228867D1 (de) 2008-10-23
GB0126887D0 (en) 2002-01-02
EP1442134A2 (en) 2004-08-04
US8852866B2 (en) 2014-10-07
CA2465377A1 (en) 2003-05-15
US20050019773A1 (en) 2005-01-27
AU2002341204B2 (en) 2006-10-19
JP2005508194A (ja) 2005-03-31
CA2465377C (en) 2012-04-03
JP4303112B2 (ja) 2009-07-29
ATE408029T1 (de) 2008-09-15
WO2003040391A2 (en) 2003-05-15
WO2003040391A3 (en) 2003-11-13
US20150065382A1 (en) 2015-03-05
EP1442134B1 (en) 2008-09-10

Similar Documents

Publication Publication Date Title
DK1453850T3 (da) EGVIII-endoglukanase og nukleinsyrer kodende for det samme
WO2003060084A3 (en) Methods for making polynucleotides and purifying double-stranded polynucleotides
EP1365025A4 (en) AGAROSIS AND CORRESPONDING GENE
DK1442134T3 (da) Fremgangsmåde til fremstilling og identifikation af oplöselige proteindomæner
DK1673457T3 (da) Nye svampeproteiner og nukleinsyrer, der koder for disse
DK372086D0 (da) Fremgangsmaade til rekombinant-dna-syntese af en serinproteaseinhibitor og dna-sekvenser, som er nyttige derved
WO2004056986A3 (en) Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
WO2004005482A3 (en) Methods and compositions for the production, identification and purification of fusion proteins
NO20010300D0 (no) Nye fremgangsmåter for identifisering av ligand og målbiomolekyler
ATE474922T1 (de) Fluoreszenzproteine aus copepoda-spezies und verfahren zur verwendung davon
AU2002252999A1 (en) Method of producing recombinant antibodies against tumors
WO2003022868A3 (en) Metal binding proteins and associated methods
DK1597369T3 (da) Anvendelse af caspaseenzymer til modning af manipulerede rekombinante polypeptidfusioner
AU4917300A (en) Method for producing a recombinant protein
PT1366161E (pt) Anticorpo anatagonista anti-pro842
WO2002002771A3 (en) Human sparc-homologous (hsparc-h1) gene and methods and uses thereof
WO2001048247A3 (en) METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION
DE60235645D1 (de) Nukleinsäure die für ein gewünschtes protein kodieren
DK1078072T3 (da) Nye peptidfragmenter til oprensning af proteiner
EP0950711A3 (en) Gonadotropin receptor
EP0688224A4 (en) MUTANT PROTEIN, METHODS AND MATERIALS USED FOR ITS MANUFACTURE AND USE
WO2003076569A3 (en) Novel polypeptides having sequence similarity to gdnfr and nucleic acids encoding the same
WO2004109579A3 (en) Protein identification methods and systems
WO2002020786A3 (en) Kinase d interacting protein
WO2004065554A3 (en) Human saa3 nucleic acid molecule, protein, and methods of use for same