DK142382B - Fremgangsmåde til bestemmelse af en sekundær alfa-aminosyre og testsystem til anvendelse dertil. - Google Patents
Fremgangsmåde til bestemmelse af en sekundær alfa-aminosyre og testsystem til anvendelse dertil. Download PDFInfo
- Publication number
- DK142382B DK142382B DK562473AA DK562473A DK142382B DK 142382 B DK142382 B DK 142382B DK 562473A A DK562473A A DK 562473AA DK 562473 A DK562473 A DK 562473A DK 142382 B DK142382 B DK 142382B
- Authority
- DK
- Denmark
- Prior art keywords
- amino acids
- bromine
- gives
- agent
- amino acid
- Prior art date
Links
- 235000008206 alpha-amino acids Nutrition 0.000 title claims description 36
- 238000000034 method Methods 0.000 title claims description 23
- 125000003275 alpha amino acid group Chemical group 0.000 title claims 3
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 claims description 18
- 229910052794 bromium Inorganic materials 0.000 claims description 18
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 claims description 17
- 150000001875 compounds Chemical class 0.000 claims description 17
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 16
- 239000003795 chemical substances by application Substances 0.000 claims description 16
- 239000000460 chlorine Substances 0.000 claims description 16
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 14
- 229910052801 chlorine Inorganic materials 0.000 claims description 14
- JRNVZBWKYDBUCA-UHFFFAOYSA-N N-chlorosuccinimide Chemical compound ClN1C(=O)CCC1=O JRNVZBWKYDBUCA-UHFFFAOYSA-N 0.000 claims description 12
- 150000001370 alpha-amino acid derivatives Chemical group 0.000 claims description 12
- 125000004432 carbon atom Chemical group C* 0.000 claims description 10
- 239000000243 solution Substances 0.000 claims description 10
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 claims description 8
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052736 halogen Inorganic materials 0.000 claims description 8
- 150000002367 halogens Chemical group 0.000 claims description 8
- 229960002591 hydroxyproline Drugs 0.000 claims description 8
- PCLIMKBDDGJMGD-UHFFFAOYSA-N N-bromosuccinimide Chemical group BrN1C(=O)CCC1=O PCLIMKBDDGJMGD-UHFFFAOYSA-N 0.000 claims description 6
- 125000000217 alkyl group Chemical group 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 125000004122 cyclic group Chemical group 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 125000003545 alkoxy group Chemical group 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 239000012736 aqueous medium Substances 0.000 claims description 3
- 125000003118 aryl group Chemical group 0.000 claims description 3
- 239000000872 buffer Substances 0.000 claims description 3
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 3
- 125000002015 acyclic group Chemical group 0.000 claims description 2
- 125000001246 bromo group Chemical group Br* 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 claims description 2
- 125000001424 substituent group Chemical group 0.000 claims description 2
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 claims 2
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims 1
- 239000004793 Polystyrene Substances 0.000 claims 1
- 239000007979 citrate buffer Substances 0.000 claims 1
- 229920000642 polymer Polymers 0.000 claims 1
- 229920002223 polystyrene Polymers 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 150000001371 alpha-amino acids Chemical group 0.000 description 21
- 238000006243 chemical reaction Methods 0.000 description 15
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 150000003141 primary amines Chemical class 0.000 description 15
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 14
- 235000001014 amino acid Nutrition 0.000 description 11
- 150000001413 amino acids Chemical class 0.000 description 10
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- 238000005658 halogenation reaction Methods 0.000 description 6
- 238000006460 hydrolysis reaction Methods 0.000 description 6
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 5
- 239000007795 chemical reaction product Substances 0.000 description 4
- 230000026030 halogenation Effects 0.000 description 4
- 150000002466 imines Chemical class 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 108010077895 Sarcosine Proteins 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 238000006114 decarboxylation reaction Methods 0.000 description 3
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 3
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 229940043230 sarcosine Drugs 0.000 description 3
- 125000000467 secondary amino group Chemical group [H]N([*:1])[*:2] 0.000 description 3
- ZVJHJDDKYZXRJI-UHFFFAOYSA-N 1-Pyrroline Chemical group C1CC=NC1 ZVJHJDDKYZXRJI-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 125000003003 spiro group Chemical group 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- FRYOUKNFWFXASU-UHFFFAOYSA-N 2-(methylamino)acetic acid Chemical compound CNCC(O)=O.CNCC(O)=O FRYOUKNFWFXASU-UHFFFAOYSA-N 0.000 description 1
- RWKSIKQHBHYHCA-UHFFFAOYSA-N 2-n-bromobenzene-1,2-dicarboxamide Chemical compound NC(=O)C1=CC=CC=C1C(=O)NBr RWKSIKQHBHYHCA-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical compound FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 description 1
- -1 acyclic amino acid Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000006473 carboxylation reaction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 125000002541 furyl group Chemical group 0.000 description 1
- 125000002883 imidazolyl group Chemical group 0.000 description 1
- 125000001041 indolyl group Chemical group 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 125000000842 isoxazolyl group Chemical group 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- VBTQNRFWXBXZQR-UHFFFAOYSA-N n-bromoacetamide Chemical compound CC(=O)NBr VBTQNRFWXBXZQR-UHFFFAOYSA-N 0.000 description 1
- 125000001624 naphthyl group Chemical group 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 125000002971 oxazolyl group Chemical group 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 125000004076 pyridyl group Chemical group 0.000 description 1
- 125000000714 pyrimidinyl group Chemical group 0.000 description 1
- 125000000168 pyrrolyl group Chemical group 0.000 description 1
- 125000005493 quinolyl group Chemical group 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000001544 thienyl group Chemical group 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6806—Determination of free amino acids
- G01N33/6809—Determination of free amino acids involving fluorescent derivatizing reagents reacting non-specifically with all amino acids
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US00298680A US3830629A (en) | 1972-10-18 | 1972-10-18 | Fluorometric analysis of secondary alpha-amino acids |
| US29868072 | 1972-10-18 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK142382B true DK142382B (da) | 1980-10-20 |
| DK142382C DK142382C (enExample) | 1981-03-16 |
Family
ID=23151560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK562473AA DK142382B (da) | 1972-10-18 | 1973-10-17 | Fremgangsmåde til bestemmelse af en sekundær alfa-aminosyre og testsystem til anvendelse dertil. |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US3830629A (enExample) |
| JP (1) | JPS4988590A (enExample) |
| AU (1) | AU461986B2 (enExample) |
| BE (1) | BE806180A (enExample) |
| CA (1) | CA1004580A (enExample) |
| CH (1) | CH621875A5 (enExample) |
| DK (1) | DK142382B (enExample) |
| FR (1) | FR2204303A5 (enExample) |
| GB (1) | GB1407177A (enExample) |
| IN (1) | IN138599B (enExample) |
| IT (1) | IT1014509B (enExample) |
| NL (1) | NL158934B (enExample) |
| SE (1) | SE394144B (enExample) |
| ZA (1) | ZA737204B (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3892530A (en) * | 1974-09-23 | 1975-07-01 | Hoffmann La Roche | Colorimetric and fluorometric method |
| DE2965740D1 (en) * | 1978-11-10 | 1983-07-28 | Hoffmann La Roche | Method for the determination of an amino acid or an amino sulfonic acid |
| US4572831A (en) * | 1982-10-19 | 1986-02-25 | Rosen Gerald M | Skin-marking compositions and devices, and their use |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3689221A (en) * | 1970-10-21 | 1972-09-05 | Hoffmann La Roche | Fluorometric assay of primary amines |
-
1972
- 1972-10-18 US US00298680A patent/US3830629A/en not_active Expired - Lifetime
-
1973
- 1973-09-10 ZA ZA737204*A patent/ZA737204B/xx unknown
- 1973-09-12 IN IN2089/CAL/73A patent/IN138599B/en unknown
- 1973-09-13 CA CA180,934A patent/CA1004580A/en not_active Expired
- 1973-09-17 CH CH1332973A patent/CH621875A5/de not_active IP Right Cessation
- 1973-09-24 IT IT29275/73A patent/IT1014509B/it active
- 1973-09-26 AU AU60750/73A patent/AU461986B2/en not_active Expired
- 1973-10-12 NL NL7314087.A patent/NL158934B/xx not_active IP Right Cessation
- 1973-10-16 JP JP48115398A patent/JPS4988590A/ja active Pending
- 1973-10-16 FR FR7336879A patent/FR2204303A5/fr not_active Expired
- 1973-10-17 DK DK562473AA patent/DK142382B/da unknown
- 1973-10-17 BE BE136775A patent/BE806180A/xx unknown
- 1973-10-17 SE SE7314141A patent/SE394144B/xx unknown
- 1973-10-17 GB GB4838773A patent/GB1407177A/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DK142382C (enExample) | 1981-03-16 |
| AU461986B2 (en) | 1975-06-12 |
| NL7314087A (enExample) | 1974-04-22 |
| FR2204303A5 (enExample) | 1974-05-17 |
| JPS4988590A (enExample) | 1974-08-23 |
| ZA737204B (en) | 1974-08-28 |
| BE806180A (fr) | 1974-04-17 |
| GB1407177A (en) | 1975-09-24 |
| CH621875A5 (enExample) | 1981-02-27 |
| CA1004580A (en) | 1977-02-01 |
| NL158934B (nl) | 1978-12-15 |
| US3830629A (en) | 1974-08-20 |
| IN138599B (enExample) | 1976-02-28 |
| IT1014509B (it) | 1977-04-30 |
| AU6075073A (en) | 1975-03-27 |
| DE2350179B2 (de) | 1976-01-22 |
| DE2350179A1 (de) | 1974-05-02 |
| SE394144B (sv) | 1977-06-06 |
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