DK1337652T3 - Manipulation af phenolsyre-indholdet og nedbrydeligheden af plantecellevægge ved målrettet ekspression af gener kodende for cellevægsnedbrydende enzymer - Google Patents

Manipulation af phenolsyre-indholdet og nedbrydeligheden af plantecellevægge ved målrettet ekspression af gener kodende for cellevægsnedbrydende enzymer

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Publication number
DK1337652T3
DK1337652T3 DK01273842T DK01273842T DK1337652T3 DK 1337652 T3 DK1337652 T3 DK 1337652T3 DK 01273842 T DK01273842 T DK 01273842T DK 01273842 T DK01273842 T DK 01273842T DK 1337652 T3 DK1337652 T3 DK 1337652T3
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Denmark
Prior art keywords
degradability
manipulation
acid content
genes encoding
degrading enzymes
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DK01273842T
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English (en)
Inventor
Nigel Dunn-Coleman
Timothy Langdon
Phillip Morris
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Genencor Int
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Publication of DK1337652T3 publication Critical patent/DK1337652T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • C12N9/2482Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01073Feruloyl esterase (3.1.1.73)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
DK01273842T 2000-11-17 2001-11-16 Manipulation af phenolsyre-indholdet og nedbrydeligheden af plantecellevægge ved målrettet ekspression af gener kodende for cellevægsnedbrydende enzymer DK1337652T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24960800P 2000-11-17 2000-11-17
PCT/US2001/043588 WO2002068666A1 (en) 2000-11-17 2001-11-16 Manipulation of the phenolic acid content and digestibility of plant cell walls by targeted expression of genes encoding cell wall degrading enzymes

Publications (1)

Publication Number Publication Date
DK1337652T3 true DK1337652T3 (da) 2009-07-27

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK01273842T DK1337652T3 (da) 2000-11-17 2001-11-16 Manipulation af phenolsyre-indholdet og nedbrydeligheden af plantecellevægge ved målrettet ekspression af gener kodende for cellevægsnedbrydende enzymer
DK09003660.9T DK2088200T3 (da) 2000-11-17 2001-11-16 Manipulation af phenolsyreindholdet og fordøjelighed af plantecellevægge ved målrettet ekspression af gener kodende for cellevægsnedbrydende enzymer.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK09003660.9T DK2088200T3 (da) 2000-11-17 2001-11-16 Manipulation af phenolsyreindholdet og fordøjelighed af plantecellevægge ved målrettet ekspression af gener kodende for cellevægsnedbrydende enzymer.

Country Status (8)

Country Link
US (2) US7132589B2 (da)
EP (2) EP1337652B1 (da)
AT (2) ATE540120T1 (da)
CA (1) CA2429347C (da)
DE (1) DE60138477D1 (da)
DK (2) DK1337652T3 (da)
ES (1) ES2325908T3 (da)
WO (1) WO2002068666A1 (da)

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US7951557B2 (en) 2003-04-27 2011-05-31 Protalix Ltd. Human lysosomal proteins from plant cell culture
US7718415B1 (en) 2003-09-23 2010-05-18 Pioneer Hi-Bred International, Inc. Acetyl esterase producing strains and methods of using same
WO2005099440A2 (en) * 2004-04-13 2005-10-27 John Burr, Iii Methods for recovering products from plants
WO2006022238A1 (ja) * 2004-08-25 2006-03-02 Hitachi Medical Corporation 超音波診断装置
CA2882532C (en) 2004-09-01 2016-06-07 Victor Nsereko Ferulate esterase producing strain lactobacillus crispatus li2350 and methods of using same as a silage inoculant
WO2008064025A2 (en) * 2006-11-13 2008-05-29 Pioneer Hi-Bred International, Inc. Cloning and sequencing of the ferulate esterase gene from lactobacillus buchneri ln4017
US20080138463A1 (en) * 2006-12-11 2008-06-12 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN5689 and its use to improve aerobic stability of silage
US20090028992A1 (en) * 2006-12-11 2009-01-29 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN1286 and its use to improve aerobic stability of silage
US20090028993A1 (en) * 2006-12-11 2009-01-29 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN1326 and its use to improve aerobic stability of silage
US20080138462A1 (en) * 2006-12-11 2008-06-12 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN5665 and its use to improve aerobic stability of silage
US20090028991A1 (en) * 2006-12-11 2009-01-29 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN1284 and its use to improve aerobic stability of silage
US20080138461A1 (en) * 2006-12-11 2008-06-12 Pioneer Hi-Bred International, Inc. Lactobacillus buchneri strain LN1297 and its use to improve aerobic stability of silage
SI2150608T1 (en) 2007-05-07 2018-04-30 Protalix Ltd. An extensive disposable bioreactor
BRPI0821088A2 (pt) * 2007-12-07 2015-09-15 Novozymes As construção de ácido nucléico, célula hospedeira microbiana transgênica, e, métodos para produzir um polipeptídeo tendo atividade de feruloil esterase, para produzir um mutante de uma célula-mãe, para inibir a expressão de um polipeptídeo tendo atividade de feruloil esterase em uma célula, para produzir uma proteína, e, para degradar um material contendo xilano.
US20100017916A1 (en) * 2008-05-30 2010-01-21 Edenspace Systems Corporation Systems for reducing biomass recalcitrance
US8058513B2 (en) * 2008-11-10 2011-11-15 Novozymes, Inc. Polypeptides having feruloyl esterase activity and polynucleotides encoding same
WO2010091337A1 (en) 2009-02-06 2010-08-12 Cornell University Trichoderma strains that induce resistance to plant diseases and/or increase plant growth
US8569465B2 (en) * 2009-07-06 2013-10-29 Wisconsin Alumni Research Foundation Method for modifying lignin structure using monolignol ferulate conjugates
JP2011087710A (ja) * 2009-10-21 2011-05-06 Toshiba Corp 超音波診断装置
ES2561553T3 (es) 2010-07-22 2016-02-26 Unilever N.V. Combinaciones de ramnolípidos y enzimas para una limpieza mejorada
EP3138919B1 (en) 2010-07-23 2018-12-19 Board of Trustees of Michigan State University Feruloyl-coa:monolignol-transferase
AU2012318626B2 (en) 2011-10-06 2017-07-20 Board Of Trustees Of Michigan State University Hibiscus cannabinus feruloyl-CoA:monolignol transferase
US10428342B2 (en) 2011-12-16 2019-10-01 Board Of Trustees Of Michigan State University P-coumaroyl-CoA:monolignol transferase
US9822334B2 (en) 2014-03-07 2017-11-21 Pioneer Hi-Bred International, Inc. Rapid acting lactobacillus strains and their use to improve aerobic stability of silage
JP7388628B2 (ja) * 2019-05-15 2023-11-29 雪印種苗株式会社 セルロース分解能増強組成物及びセルロース分解能増強変異株
CN110423704A (zh) * 2019-06-14 2019-11-08 青岛蔚蓝生物集团有限公司 一种高产阿魏酸酯酶的毕赤酵母菌株

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Also Published As

Publication number Publication date
DE60138477D1 (de) 2009-06-04
WO2002068666A1 (en) 2002-09-06
ATE540120T1 (de) 2012-01-15
WO2002068666A8 (en) 2003-11-06
US7453023B2 (en) 2008-11-18
CA2429347C (en) 2014-07-08
ATE429501T1 (de) 2009-05-15
ES2325908T3 (es) 2009-09-24
US20060005270A1 (en) 2006-01-05
DK2088200T3 (da) 2012-04-23
US20030024009A1 (en) 2003-01-30
EP1337652B1 (en) 2009-04-22
EP1337652A1 (en) 2003-08-27
EP2088200A1 (en) 2009-08-12
CA2429347A1 (en) 2002-09-06
EP2088200B1 (en) 2012-01-04
US7132589B2 (en) 2006-11-07

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