WO2003043411A3 - Manipulation of the phenolic acid content and digestibility of plant cell walls - Google Patents

Manipulation of the phenolic acid content and digestibility of plant cell walls Download PDF

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Publication number
WO2003043411A3
WO2003043411A3 PCT/US2002/016239 US0216239W WO03043411A3 WO 2003043411 A3 WO2003043411 A3 WO 2003043411A3 US 0216239 W US0216239 W US 0216239W WO 03043411 A3 WO03043411 A3 WO 03043411A3
Authority
WO
WIPO (PCT)
Prior art keywords
digestibility
manipulation
plant cell
acid content
cell walls
Prior art date
Application number
PCT/US2002/016239
Other languages
French (fr)
Other versions
WO2003043411A2 (en
Inventor
Nigel Dunn-Coleman
Timothy Langdon
Phillip Morris
Original Assignee
Genencor Int
Nigel Dunn-Coleman
Timothy Langdon
Philip Morris Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2001/043588 external-priority patent/WO2002068666A1/en
Application filed by Genencor Int, Nigel Dunn-Coleman, Timothy Langdon, Philip Morris Inc filed Critical Genencor Int
Priority to AU2002305678A priority Critical patent/AU2002305678A1/en
Publication of WO2003043411A2 publication Critical patent/WO2003043411A2/en
Publication of WO2003043411A3 publication Critical patent/WO2003043411A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Abstract

Described herein are methods to enhance the production of more highly fermentable carbohydrates in plants, especially forage grasses. The invention provides for transgenic plants transformed with expression vectors containing a DNA sequence encoding ferulic acid esterase I from Aspergillus, preferably A. niger. The expression vectors may optionally comprise a DNA sequence encoding xylanase from Trichoderma, preferably T. reesei. Expression of the enzyme(s) is targeted to specific cellular compartments, in specific tissues and under specific environmental conditions. Uses of this invention include, but are not limited to, forage with improved digestibility for livestock, and enhanced biomass conversion.
PCT/US2002/016239 2001-11-16 2002-05-21 Manipulation of the phenolic acid content and digestibility of plant cell walls WO2003043411A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002305678A AU2002305678A1 (en) 2001-11-16 2002-05-21 Manipulation of the phenolic acid content and digestibility of plant cell walls

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
USPCT/US01/43588 2001-11-16
PCT/US2001/043588 WO2002068666A1 (en) 2000-11-17 2001-11-16 Manipulation of the phenolic acid content and digestibility of plant cell walls by targeted expression of genes encoding cell wall degrading enzymes
US09/991,209 US7132589B2 (en) 2000-11-17 2001-11-16 Manipulation of the phenolic acid content and digestibility of plant cell walls by targeted expression of genes encoding cell wall degrading enzymes
US09/991,209 2001-11-16

Publications (2)

Publication Number Publication Date
WO2003043411A2 WO2003043411A2 (en) 2003-05-30
WO2003043411A3 true WO2003043411A3 (en) 2004-02-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/016239 WO2003043411A2 (en) 2001-11-16 2002-05-21 Manipulation of the phenolic acid content and digestibility of plant cell walls

Country Status (2)

Country Link
AU (1) AU2002305678A1 (en)
WO (1) WO2003043411A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005279724B2 (en) 2004-09-01 2010-03-25 Pioneer Hi-Bred International, Inc. Ferulate esterase producing strains and methods of using same
US7919683B2 (en) 2006-11-13 2011-04-05 Pioneer Hi-Bred International, Inc. Cloning and sequencing of the ferulate esterase gene from Lactobacillus buchneri LN4017
WO2009065934A1 (en) * 2007-11-22 2009-05-28 Novozymes A/S Polypeptides having ferulic acid esterase activity and polynucleotides encoding same
US8716001B2 (en) 2009-02-06 2014-05-06 Cornell University Trichoderma strains that induce resistance to plant diseases and/or increase plant growth
US9822334B2 (en) 2014-03-07 2017-11-21 Pioneer Hi-Bred International, Inc. Rapid acting lactobacillus strains and their use to improve aerobic stability of silage

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2301103A (en) * 1995-05-23 1996-11-27 Danisco Enzyme system comprising ferulic acid esterase activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2301103A (en) * 1995-05-23 1996-11-27 Danisco Enzyme system comprising ferulic acid esterase activity

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DARNOWSKI D.W. ET AL.: "A soybean lectin-GFP fusion labels the vacuoles in developing arabidopsis thaliana embryos", PLANT CELL REPORTS, vol. 20, no. 11, May 2002 (2002-05-01), pages 1033 - 1038, XP002970103 *
DE VRIES R.P. ET AL.: "The faeA genes from aspergillus niger and aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 63, no. 12, 1997, pages 4638 - 4644, XP002203731 *

Also Published As

Publication number Publication date
AU2002305678A8 (en) 2003-06-10
AU2002305678A1 (en) 2003-06-10
WO2003043411A2 (en) 2003-05-30

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