DE939398C - Fertilizer for bacteria - Google Patents

Fertilizer for bacteria

Info

Publication number
DE939398C
DE939398C DEC3298A DEC0003298A DE939398C DE 939398 C DE939398 C DE 939398C DE C3298 A DEC3298 A DE C3298A DE C0003298 A DEC0003298 A DE C0003298A DE 939398 C DE939398 C DE 939398C
Authority
DE
Germany
Prior art keywords
bacteria
tubercle bacilli
water
culture media
fertilizer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEC3298A
Other languages
German (de)
Inventor
Armin Dr Kutzsche
Otto Dr Troesken
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cassella Farbwerke Mainkur AG
Original Assignee
Cassella Farbwerke Mainkur AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cassella Farbwerke Mainkur AG filed Critical Cassella Farbwerke Mainkur AG
Priority to DEC3298A priority Critical patent/DE939398C/en
Application granted granted Critical
Publication of DE939398C publication Critical patent/DE939398C/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Description

Nährböden für Bakterien Die üblichen Bakteriennährböden haben die unangenehme Eigenschaft, daß sie das Wachstum von Bakterien, insbesondere von Tuberkelbazillen, nicht genügend fördern. Man hat deshalb schon versucht, durch Zusatz esterartiger Netzmittel diese Nährböden zu verbessern, konnte aber auch dadurch nicht vermeiden, daß die darauf gezüchteten Keime nach mehrmaliger Passage degenerieren.Culture media for bacteria The usual bacteria culture media have the unpleasant property that it prevents the growth of bacteria, especially tubercle bacilli, not promote enough. Attempts have therefore already been made to add ester-like Wetting agents to improve these nutrient media, but could not avoid that the germs grown on it degenerate after repeated passage.

Ein solches esterartiges Netzmittel ist z. B. der bekannte Monoester der ölsäure mit Polyäthylenoxydsorbit (s. Zentralblatt 1948, Westausgabe, I, S. 399, Referat einer Abhandlung von Bloch, Erlenmeyer und Suter).Such an ester-like wetting agent is e.g. B. the well-known monoester oleic acid with polyethylene oxide sorbitol (see Zentralblatt 1948, Westausgabe, I, p. 399, presentation of a treatise by Bloch, Erlenmeyer and Suter).

Dieses Präparat spaltet Säure ab, welche das Bakterienwachstum schädlich beeinflussen würde, und man ist daher gezwungen, die Säurewirkung durch Zugabe von Albumin zu paralysieren. Letzteres liefert jedoch mit der Säure Präzipitate in den Nährböden, welche eine positive Bakterienkultur vortäuschen, von der sie nur durch laufende mikroskopische Untersuchungen zu unterscheiden sind.This preparation breaks down acids, which are harmful to bacterial growth would affect, and one is therefore forced to reduce the acidic effect by adding Paralyze albumin. The latter, however, produces precipitates in the with the acid Culture media, which simulate a positive bacterial culture, from which they only come through ongoing microscopic examinations are to be distinguished.

Es wurde nun gefunden, daß diese Nachteile sich dadurch beseitigen lassen, daß man den üblichen Bakteriennährböden wasserlösliche, ätherartige Kondensationsprodukte von Alkylenoxyden mit höhermolekularen Alkoholen zusetzt.It has now been found that these disadvantages are eliminated let the usual bacterial culture media water-soluble, ethereal condensation products of alkylene oxides with higher molecular weight alcohols added.

Es bedeutet in diagnostischer Hinsicht den großen Vorzug, daß man in den Nährböden gemäß der Erfindung nach der Beimpfung mit dem Unter- Buchungsmaterial auftretende Korpuskel ohne besondere Hilfsmittel und eindeutig als positive Bakterienkultur erkennen kann.From a diagnostic point of view it means the great advantage that one in the nutrient media according to the invention after inoculation with the under- Booking material corpuscles appearing without special aids and clearly as a positive bacterial culture can recognize.

Beispiel Aus to Teilen löslicher Phosphate, 4 Teilen anorganischer und organischer Stickstoffquellen, o,15 Teilen des Kondensationsproduktes aus Stearylalkohol mit 40 Mol Äthylenoxyd, geringen Mengen von Metallsalzen und der nötigen Menge destillierten Wassers werden zooo Teile eines flüssigen Nährbodens hergestellt.Example From to parts of soluble phosphates, 4 parts of inorganic and organic nitrogen sources, 0.15 parts of the condensation product of stearyl alcohol distilled with 40 moles of ethylene oxide, small amounts of metal salts and the necessary amount Water, zooo parts of a liquid nutrient medium are produced.

Der so erhaltene Nährboden läßt kleinste Mengen von Bakterien angehen, befördert diese außerordentlich in ihrem Wachstum, das diffus erfolgt, und schädigt die Bakterien auch nicht bei zahlreichen Passagen.The resulting culture medium can attack the smallest amounts of bacteria, promotes this extraordinarily in its growth, which takes place diffusely, and damages the bacteria also not in numerous passages.

Verwendet man statt des angegebenen Kondensationsproduktes das Kondensationsprodukt aus Oleylalkohol mit 27 Mol Äthylenoxyd oder, aus Cetylalkohöl mit 30 Mol Äthylenoxyd öder aus Triisopropylbenzylalkohol mit 36 Mol Äthylenoxyd (hergestellt nach dem Verfahren des Patents 925 230) in gleicher bis doppelter Menge, so erhält man Nährboden mit ähnlichen guten Eigenschaften.If, instead of the specified condensation product, the condensation product of oleyl alcohol with 27 mol of ethylene oxide or of cetyl alcohol with 30 mol of ethylene oxide or of triisopropylbenzyl alcohol with 36 mol of ethylene oxide (prepared according to the process of patent 925 230) is used in the same or double the amount, one obtains a culture medium with similar good properties.

Claims (1)

PATENTANSPRUCH: Nährböden für Bakterien, insbesondere Tuberkelbazillen, dadurch gekennzeichnet, daß sie außer Wasser und den üblichen Bakteriennährstoffen wasserlösliche ätherartige Kondensationsprodukte von Alkylenoxyden mit höhermolekularen Alkoholen enthalten. . Angezogene Druckschriften: Referat der Arbeit von B loch, E r l e n m ey e r und S u te r : »Zur Austestung von Wachstumsinhibitoren für Tuberkelbazillen in vitro« im Chem. Zentralblatt 1948 (Westausgabe), I, S. 399ePATENT CLAIM: Culture media for bacteria, especially tubercle bacilli, characterized in that they contain water and the usual bacterial nutrients water-soluble ethereal condensation products of alkylene oxides with higher molecular weight Contain alcohols. . Printed publications: Presentation of the work by B loch, E r l e n m ey e r and S u te r: »For testing growth inhibitors for tubercle bacilli in vitro "in Chem. Zentralblatt 1948 (western edition), I, p. 399e
DEC3298A 1950-10-19 1950-10-19 Fertilizer for bacteria Expired DE939398C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEC3298A DE939398C (en) 1950-10-19 1950-10-19 Fertilizer for bacteria

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEC3298A DE939398C (en) 1950-10-19 1950-10-19 Fertilizer for bacteria

Publications (1)

Publication Number Publication Date
DE939398C true DE939398C (en) 1956-02-23

Family

ID=7013018

Family Applications (1)

Application Number Title Priority Date Filing Date
DEC3298A Expired DE939398C (en) 1950-10-19 1950-10-19 Fertilizer for bacteria

Country Status (1)

Country Link
DE (1) DE939398C (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

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