DE928317C - Process for the production of antibiotic active ingredients - Google Patents

Process for the production of antibiotic active ingredients

Info

Publication number
DE928317C
DE928317C DEJ582A DEJ0000582A DE928317C DE 928317 C DE928317 C DE 928317C DE J582 A DEJ582 A DE J582A DE J0000582 A DEJ0000582 A DE J0000582A DE 928317 C DE928317 C DE 928317C
Authority
DE
Germany
Prior art keywords
extracted
production
antibiotic active
active ingredients
ether
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEJ582A
Other languages
German (de)
Inventor
Erich Dr Med Hesse
Walter Dr Rer Nat Rudolph
Gustav Dr Rer Nat Renckhoff
Karl-Heinz Dr Rer Nat Imhausen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Imhausen & Co GmbH
KARL HEINZ IMHAUSEN DR
Original Assignee
Imhausen & Co GmbH
KARL HEINZ IMHAUSEN DR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Imhausen & Co GmbH, KARL HEINZ IMHAUSEN DR filed Critical Imhausen & Co GmbH
Priority to DEJ582A priority Critical patent/DE928317C/en
Application granted granted Critical
Publication of DE928317C publication Critical patent/DE928317C/en
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Description

Verfahren zur Gewinnung von antibiotischen Wirkstoffen Gegenstand eines älteren Rechts ist ein Verfahren zur Gewinnung von antibiotischen Wirkstoffen, bei dem mit tuberkuloaktiven Stämmen der Gruppe Escherichia coli bebrütete Nährlösungen als Ausgangsmaterial für die Isolierung der Wirkstoffe benutzt wurden.Process for the production of antibiotic active substances subject an earlier right is a process for the production of antibiotic active substances, in the case of the nutrient solutions incubated with tuberculoactive strains of the Escherichia coli group were used as starting material for the isolation of the active substances.

Es wurde nun gefunden, daß sich durch Extraktion der Nährlösungen mit lipoidlösenden Flüssigkeiten eine erhebliche Konzentration der Wirkstoffe erzielen läßt. Als Extraktionsmittel ist insbesondere Äther geeignet, aber auch mit Petroläther, Methylenchlorid, Äthylacetat, Methyläthylketon, Butanol u. dgl. läßt sich das wirksame Prinzip extrahieren.It has now been found that by extracting the nutrient solutions Achieve a significant concentration of the active ingredients with lipoid-dissolving liquids leaves. Ether is particularly suitable as an extractant, but also petroleum ether, Methylene chloride, ethyl acetate, methyl ethyl ketone, butanol and the like can be the effective Extract principle.

Die Extraktion wird zweckmäßig bei Temperaturen unterhalb etwa 45° vorgenommen; falls kontinuierlich unter Verdampfung des Lösungsmittels aus der Extraktlösung gearbeitet wird, wird daher der Druck so weit erniedrigt, daß die Siedetemperatur der Extraktlösung 45° nicht übersteigt. Die bebrütete Nährlösung kann so, wie sie anfällt, also schwach sauer oder schwach alkalisch, extrahiert werden; zweckmäßig wird die Extraktion bei einem pH unterhalb 7 vorgenommen.The extraction is expedient at temperatures below about 45 ° performed; if continuously with evaporation of the solvent from the extract solution is carried out, the pressure is therefore lowered so far that the boiling point the extract solution does not exceed 45 °. The incubated nutrient solution can be like them is obtained, i.e. weakly acidic or weakly alkaline, can be extracted; expedient the extraction is carried out at a pH below 7.

Wird z. B. eine 3 Wochen bebrütete Kultur tuberkuloaktiver Colistämme in Traubenzucker-Hottinger-Bouillon vom PH 4,8 erschöpfend mit Äther extrahiert, so erhält man etwa 0,5 g Extrakt pro Liter Nährlösung. Bei der Extraktion von 6 Tage bebrüteten Hottinger-Bouillonkulturen, die von den Coli befreit und auf pH 6 eingestellt waren, mit Äther fallen etwa 70 mg Extrakt pro Liter Kulturmedium an. Die Extrakte sind gelbe Öle, aus denen sich nach einiger Zeit Kristalle ausscheiden.Is z. For example, if a culture of tuberculoactive coli strains incubated for 3 weeks is extracted exhaustively with ether in glucose Hottinger broth of pH 4.8, about 0.5 g of extract per liter of nutrient solution is obtained. In the extraction of Hottinger broth cultures which have been incubated for 6 days, which have been freed from the Coli and adjusted to pH 6, with ether, about 70 mg of extract per liter of culture medium are obtained. The extracts are yellow oils, from which crystals separate out after a while.

Die gewonnenen, vom Extraktionsmittel befreiten Extrakte sind beim Lochtest und Diffusionstest gegen humane und Kaltblüterbazillen sowie beim Kontaktversuch auf Sautonnährboden kräftig tuberkulocid und sind auch wirksam gegen die Erreger des Paratyphus, Typhus, gegen Streptokokken u. dgl.The extracts obtained and freed from the extractant are at Hole test and diffusion test against human and cold-blooded bacilli as well as in contact tests vigorously tuberculocid on sauton culture medium and are also effective against the Pathogen of paratyphus, typhus, against streptococci and the like.

Claims (4)

PATENTANSPRÜCHE: z. Verfahren zur Gewinnung von antibiotischen Wirkstoffen durch Aufarbeitung von mit tuberkuloaktiven Stämmen der Gruppe Escherichia coli bebrüteten Nährlösungen, dadurch gekennzeichnet, daß die gegebenenfalls von Bakterien befreiten Nährlösungen mit lipoidlösenden Lösungsmitteln extrahiert werden. PATENT CLAIMS: e.g. Process for the production of antibiotic active substances by processing with tuberculoactive strains of the group Escherichia coli incubated nutrient solutions, characterized in that, where appropriate, of bacteria freed nutrient solutions are extracted with lipoid-dissolving solvents. 2. Verfahren nach Anspruch z, . dadurch gekennzeichnet, daß mit Äther oder auch Petroläther, Methylenchlorid, Äthylacetat, Methyläthylketon, Butanol od. dgl. extrahiert wird. 2. Procedure according to claim z,. characterized in that with ether or petroleum ether, Methylene chloride, ethyl acetate, methyl ethyl ketone, butanol or the like. Is extracted. 3. Verfahren nach Anspruch r oder 2, dadurch gekennzeichnet, daß bei Temperaturen unterhalb etwa 45° extrahiert wird. 3. The method according to claim r or 2, characterized in that at temperatures is extracted below about 45 °. 4. Verfahren nach Anspruch r, 2 oder 3, dadurch gekennzeichnet, daB das pH der zu extrahierenden Nährlösung auf unter 7 eingestellt wird. Angezogene Druckschriften: Annales de 1'Institut Pasteur, 1947, S. 904 bis 9o6.4. The method according to claim r, 2 or 3, characterized characterized that the pH of the nutrient solution to be extracted is adjusted to below 7 will. Cited pamphlets: Annales de 1'Institut Pasteur, 1947, pp. 904 bis 9o6.
DEJ582A 1950-03-03 1950-03-03 Process for the production of antibiotic active ingredients Expired DE928317C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEJ582A DE928317C (en) 1950-03-03 1950-03-03 Process for the production of antibiotic active ingredients

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEJ582A DE928317C (en) 1950-03-03 1950-03-03 Process for the production of antibiotic active ingredients

Publications (1)

Publication Number Publication Date
DE928317C true DE928317C (en) 1955-05-31

Family

ID=7197475

Family Applications (1)

Application Number Title Priority Date Filing Date
DEJ582A Expired DE928317C (en) 1950-03-03 1950-03-03 Process for the production of antibiotic active ingredients

Country Status (1)

Country Link
DE (1) DE928317C (en)

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

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