DE916206C - Process for the production of protein hydrolyzate preparations from substances containing protein - Google Patents

Description

Process for the production of protein hydrolyzate preparations from protein-containing Substances The invention relates to a method for producing protein hydrolyzate preparations from protein-containing substances through the breakdown of proteins by means of proteolytic enzymes.

Processes are already known in which protein-containing substances, mostly animal organs, hydrolyzed by means of proteodytic enzymes such as papain will. Here you get largely degraded protein products, so-called Protein hydrolyzate preparations, which are used in digestive disorders or other cases which easily digestible amino acid drugs and the like are administered to the patient have to find use.

Administration is intravenous so that the protein hydrolyzate preparations must not contain any insufficiently degraded protein products or large molecules. In addition, the hydrolysis products are carefully dialyzed, which makes the products very good get expensive.

It is also already known a continuous, enzymatic Carry out cellulose degradation in a container separated by a diaphragm, whereby the glucose formed should diffuse through the diaphragm. In contrast to do this, according to the present method, not with a diaphragm, but with relatively coarse-meshed fabric sacks.

The invention relates to a method by means of which cheap, for Oral administration suitable protein hydrolyzate products of high nutritional value in technical Scope can be produced.

According to the invention, that which is to be hydrolyzed is protein-containing Starting material together with enzyme, preferably papain, in one or more Fabric sacks introduced, which are immersed in warm water, the result of the hydrolysis Protein hydrolyzate products formed get into the water and regularly with the water and are preferably discharged continuously.

It has been found that when using fabric bags, for example linen bags lined with super polyamides, the water surrounding the bags the Absorbs hydrolysis products. It is true that the larger molecules also get in here the water, however, is harmless when the protein hydrolyzate is administered orally.

The warm water surrounding the bags becomes regular and preferential slowly and continuously renewed. The hydrolysis reaction is carried out by the regular Discharge of the hydrolyzate favorably influenced and continued.

While the inventive method in principle with one in one A sack located in a warm water bath is preferably made Embodiment of the method in that in a hot water tank a plurality to accommodate from sacks. It: it is advisable to keep the sacks in constant motion to keep.

Be a number loose, filled in the manner described above and cylindrical sacks tied at the bottom in a filled with warm water, around a A tube rotating approximately horizontal axis is introduced, so the sacks roll during the pipe circulation constantly up and against each other, so that the contents of the bags regularly occupies a different position, which has a favorable influence on the hydrolysis will.

The water surrounding the bags can be uniform and preferential are continuously supplied and discharged, so that the hydrolyzates also regularly be discharged. For the purposes of the invention, any other suitable one is also under water Understood solvents for the protein hydrolysates. The bags can of course also in other ways, e.g. B. in a stationary container, are kept in motion.

It is conceivable that a layer of the mass contained in the bags settles inside the bags, which on the one hand promotes hydrolysis and on the other hand, the passage of undesirable, very large, insufficiently degraded Protein products are avoided.

In the method according to the invention, preferably animal Organs subjected to protein hydrolysis.

The temperature of the water surrounding the bags is preferably so high that the protein-containing starting material cannot spoil. Naturally, the choice of enzyme to be used depends on the temperature. So preferably P'apain is used, which is optimally effective at a higher, for the spoilage less dangerous temperature of about 70 ° C, instead of z. B. Pepsin, which is effective at around 30 ° C.

The water discharged into a reservoir can be evaporated by evaporation in a vacuum. : possibly by subsequent atomization, the protein hydrolysates in a drier Powder form can be obtained.

In practice it has been found that lined with super polyamides Body sacks are very suitable for carrying out the procedure. It recommends at the end of the hydrolysis, the water with the hydrolysis products present in it to be heated to 100 ° C or higher. This results in: any microorganisms that may be present killed, and protein still present in the water can be converted into: a Coagulate are converted.

The method according to the invention can be, for example, as follows perform: 6 to gkg dried spleen powder or 3o to 4okg freshly ground Spleen are mixed with 400 to 600 grams of papaiii. The mixture is in; a number long cylindrical body bags that are closed at both ends. The filled bags are then placed in a rotating with warm water at 70 ° C brought a filled tube of 4o1 content and kept it in the warm water for 12 hours, slowly renewing the water.

After the hydrolysis is complete, the protein hydrolyzate: containing Water is heated to 100 ° C, whereby the protein that may be present in the water coagulated, which is then filtered off.

The solution is sterilized at the same time as it is heated.

The solution is then evaporated in vacuo at 70.degree. C. until a concentration is reached which allows atomization to give a dry powder.

In addition to papain, other proteinases such as trypsin and pepsin, to be used. However, the enzymes that are effective at low temperatures have the disadvantage that they favor bacterial growth in the mass to be hydrolyzed.

Claims (1)

PATENT APPLICATION: 1. Process for the production of protein hydrolyzate preparations from protein-containing substances through the breakdown of proteins by means of proteolytic enzymes, characterized in that the protein-containing starting material, al with an enzyme, preferably papain, added and filled into one or more fabric sacks, which in turn are introduced into warm water, whereby the hydrolysis formed protein products get into the water and with the water regularly and are preferably discharged continuously. a. Method according to claim i, characterized characterized in that the sack or sacks are in motion in the surrounding water are held, preferably by the fact that the sacks are in regular contact with one another to be brought. 3. The method according to: one of claims i or a, characterized by using more cylindrical, more closed at both ends Sacks which roll freely in a rotating cylinder filled with water in Movement are kept. Method according to one of Claims i to 3, characterized in that that after the hydrolysis the water with the hydrolyzates present in it to ioo ° C and the liquid is optionally filtered. Referred publications: German patent specification No. 705010.